COS-7 Nuclear Hypoxic Induced Cell Lysate

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Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before ...read more
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were left untreated (C) or were treated with Cobalt Chloride / CoCl2 (H) before preparation ...read more
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
2.5 mg/ml

Order Details

COS-7 Nuclear Hypoxic Induced Cell Lysate Summary

Description
NB800-PC26 packaging includes 0.2 mg untreated and 0.2 mg treated of COS-7 nuclear extract. NB800-PC26 COS-7 nuclear extract was collected in Lysis Buffer after a 16-hour incubation with CoCl2 (0.15 mM).
Specificity
NB800-PC26 contains hypoxia induced and uninduced COS-7 nuclear extract lysate

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Extracts have been quality control tested by Western blot. Before use mix 1:1 with 2X Sample Buffer and heat to 90C for 5 minutes before running on gel.
Publications
Read Publications using
NB800-PC26 in the following applications:

  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20 mM Hepes (pH 7.5), 400 mM NaCl, 0.1 mM EDTA, 10 mM NaF, 10 uM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM beta-glycerophosphate, 1 mM DTT and protease inhibitors.
Preservative
20% glycerol
Concentration
2.5 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Digestive/Waste
Subcellular Fraction
Nuclear Hypoxic Induced

Notes

NB800-PC26 packaging includes 0.2 mg untreated and 0.2 mg treated of COS-7 nuclear extract. NB800-PC26 COS-7 nuclear extract was collected in Lysis Buffer after a 16-hour incubation with CoCl2 (0.15 mM).

Background

Cobalt Chloride (CoCl2) treated cells are often used as a positive control for HIF-1 and HIF-2 alpha analysis in hypoxia signaling experiments. HIFs induction can be conveniently achieved in vitro through CoCl2 treatment of cultured cells. CoCl2 inhibits prolyl-hydroxylases (PHD enzymes), the oxygen sensors, through replacement of Fe with Co making these enzymes unable to mark HIF alpha for degradation. In other words, CoCl2 allosterically blocks PHDs through its metal ion binding domain, hence, abolishing the VHL-HIF interaction which ultimately leads to the accumulation of HIFs in CoCl2 treated cells. CoCl2 has been regarded as one of the most reliable HIF-1 alpha inducer or hypoxia response mimicker, and this chemically induced hypoxia model is widely used in hypoxia-signaling research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for COS-7 Lysate (NB800-PC26)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(6)
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Human
(1)
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Showing Publications 1 - 8 of 8.
Publications using NB800-PC26 Applications Species
Manuprasert W, Leelahavanichkul A, Kanjanabuch S et al. Intermittent hypoxia in rat enhancing peritoneal membrane thickening through HIF-1?-induced cytokines in peritoneum Asian Pacific Journal of Allergy and Immunology 2022-01-01 [PMID: 31837216]
Kletkiewicz H, Hyjek M, Jaworski K et al. Activation of hypoxia-inducible factor-1a in rat brain after perinatal anoxia: role of body temperature.. Int J Hyperthermia. 2017-10-23 [PMID: 28974122] (WB, Human) WB Human
Smeyne M, Sladen P, Jiao Y et al. HIF1a is necessary for exercise-induced neuroprotection while HIF2a is needed for dopaminergic neuron survival in the substantia nigra pars compacta. Neuroscience. 2015-03-19 [PMID: 25796140] (WB) WB
Huang C, Hales BF. Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs PLoS One 2012-01-01 [PMID: 23251655] (WB) WB
Srinivasan S, Dunn JF. Stabilization of hypoxia-inducible factor-1a in buffer containing cobalt chloride for Western blot analysis Anal Biochem 2011-09-01 [PMID: 21601556] (WB) WB
Huang C, Hales BF. Teratogen responsive signaling pathways in organogenesis stage mouse limbs. Reprod Toxicol 2009-04-01 [PMID: 19429390] (WB) WB
Tam, KKY. The role of hypoxia inducible factors in regulating ovarian function. Thesis (PhD) University of Adelaide, School of Paediatrics and Reproductive Health, 2010. 2010-01-01 (WB) WB
Zhao M, Fajardo GA, Urashima T et al. Cardiac Pressure Overload Hypertrophy is Differentially Regulated by {beta}-Adrenergic Receptors. Am J Physiol Heart Circ Physiol;301(4):H1461-70. 2011-10-01 [PMID: 21705675]

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FAQs for COS-7 Lysate (NB800-PC26). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in the above product with catalog number NB800-PC26 for western blot. Can I get more information about how the lysates are being produced before proceed with the decision of purchasing? It will be good if you have any protocols and reagents used particularly in western blot application with this product.
    • NB800-PC26 packaging includes 0.2 mg untreated and 0.2 mg treated of COS-7 nuclear extract. NB800-PC26 COS-7 nuclear extract was collected in Lysis Buffer after a 16-hour incubation with CoCl2 (0.15 mM). These cells come in 20 mM Hepes (pH 7.5), 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 uM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM beta-glycerophosphate, 1 mM DTT and protease inhibitors as a buffer.

Additional COS-7 Products

Array NB800-PC26

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