iNOS Antibody

Images

 
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours ...read more
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) ...read more
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Western blot image submitted by a verified customer review.
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - LCA-induced oxidative stress in 4T1 breast cancer cells. The 4T1 cells were treated with LCA for 48 h, then the indicated measurements were ...read more
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Validated by:
   

Biological Strategies

     

iNOS Antibody Summary

Immunogen
iNOS Antibody was made to a synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.
Specificity
This antibody detects iNOS. It does not detect other NOS isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:20
  • In vitro assay
  • Western Blot 1:200 - 1:800
Application Notes
WB: Detects an approx. 135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. Also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples. Use In vitro assay reported in scientific literature (PMID: 27998907). Use in FLOW reported in scientific literature (PMID: 31536479).
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
iNOS Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB300-605 in the following applications:

Publications
Read Publications using
NB300-605 in the following applications:

  • 1 publication
  • IB
    1 publication
  • 9 publications
  • IHC
    14 publications
  • 6 publications
  • WB
    26 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 31292486).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for iNOS Antibody

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2

Background

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for iNOS Antibody (NB300-605)(69)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: Flow, IB, ICC/IF, IHC, IHC-P, WB.


Filter By Application
Flow
(1)
IB
(1)
ICC/IF
(9)
IHC
(14)
IHC-P
(6)
WB
(26)
All Applications
Filter By Species
Human
(4)
Mouse
(32)
Rat
(10)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 69. Show All 69 Publications.
Publications using NB300-605 Applications Species
Yu H, Hou G, Cao J et al. Mangiferin Alleviates Mitochondrial ROS in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration via Suppression of NF-kappa B Signaling Pathway Oxidative Medicine and Cellular Longevity Jun 11 2021 [PMID: 34234886]
Jandova J, Snell J, Hua A et al. Topical hypochlorous acid (HOCl) blocks inflammatory gene expression and tumorigenic progression in UV-exposed SKH-1 high risk mouse skin Redox biology Jun 11 2021 [PMID: 34144392] (IHC, Mouse) IHC Mouse
Li Y, Li Z, Quan Y et al. Macrophage recruitment in immune-privileged lens during capsule repair, necrotic fiber removal, and fibrosis iScience Jun 1 2021 [PMID: 34142044] (Mouse) Mouse
Sp N, Kang DY, Kim HD et al. Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-kappa B Signaling in CCD-986Sk Skin Fibroblasts Life (Basel, Switzerland) May 10 2021 [PMID: 34068523]
Mouton AJ, Flynn ER, Moak SP et al. Dimethyl fumarate preserves left ventricular infarct integrity following myocardial infarction via modulation of cardiac macrophage and fibroblast oxidative metabolism Journal of molecular and cellular cardiology May 21 2021 [PMID: 34023353]
Seo MG, Jo MJ, Hong NI et al. Anti-Inflammatory and Anti-Vascular Leakage Effects by Combination of Centella asiatica and Vitis vinifera L. Leaf Extracts Evidence-based complementary and alternative medicine : eCAM Apr 14 2021 [PMID: 33936244]
Misra S, Kilari S, Yang B et al. Anti Human CX3CR1 VHH Molecule Attenuates Venous Neointimal Hyperplasia of Arteriovenous Fistula in Mouse Model Journal of the American Society of Nephrology : JASN Apr 23 2021 [PMID: 33893223]
Zhang JQJ, Saravanabavan S, Cheng KM et al. Long-term dietary nitrate supplementation does not reduce renal cyst growth in experimental autosomal dominant polycystic kidney disease PloS one Apr 22 2021 [PMID: 33886581] (WB, Mouse) WB Mouse
Raisi A, Dezfoulian O, Davoodi F et al. Salvia miltiorrhiza hydroalcoholic extract inhibits postoperative peritoneal adhesions in rats BMC complementary medicine and therapies Apr 20 2021 [PMID: 33879143] (IHC, Rat) IHC Rat
Ma X, Zhang Z, Shen M et al. Changes of type II collagenase biomarkers on IL-1beta-induced rat articular chondrocytes Experimental and therapeutic medicine Jun 1 2021 [PMID: 33850554] (WB, Rat) WB Rat
Show All 69 Publications.

Reviews for iNOS Antibody (NB300-605) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-605:
Filter by Applications
WB
(2)
IF
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot iNOS NB300-605
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 09/15/2020
View

Summary

ApplicationWestern Blot
Sample TestedLiver tissue
SpeciesMouse
Lotqd213201
Immunofluorescence iNOS NB300-605
Enlarge
5
reviewed by:
Verified Customer
IF Mouse 11/13/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse aortic root
SpeciesMouse

Blocking

Blocking Details30 minutes in normal donkey serum/TBST room temperature

Details

Detection NotesDetected Rhodamine Red from secondary antibody using fluorescence microscope
Fixation DetailsParaffin
Western Blot iNOS NB300-605
Enlarge
5
reviewed by:
Leonid Tarassishin
WB Human 04/28/2014
View

Summary

ApplicationWestern Blot
Sample Testedhuman astrocyte lysate
SpeciesHuman
LotNJ17385 1
Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)

Blocking

Blocking Details5% non-fat milk in PBS

Primary Anitbody

Dilution Ratio1:250, 18 hr, 4C, PBST (PBS+0.05%Tween 20)

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#Pierce/ThermoScientific, Cat. # 32460
Secondary Concentration1:500 (stock 10 ug/ml)

Details

Detection NotesECL-Pico (Piere/ThermoScientific), 1 min; Untreated and treated with IL-1beta cells; Loading Ctr: beta-Actin.

Comments

Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for iNOS Antibody (NB300-605). (Showing 1 - 2 of 2 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. Would there be any interference from other iNOS isoforms on a Western blot or any other method for NB300-605?
    • NB300-605 will only recognize the iNOS isoform, so there should be no interference from eNOS, nNOS or bNOS.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB300-605AF405
Alexa Fluor 488 NB300-605AF488
Alexa Fluor 647 NB300-605AF647
Alexa Fluor 700 NB300-605AF700
Biotin NB300-605B
DyLight 350 NB300-605UV
DyLight 405 NB300-605V
DyLight 488 NB300-605G
DyLight 550 NB300-605R
DyLight 594 NB300-605DL594
DyLight 650 NB300-605C
DyLight 680 NB300-605FR
DyLight 755 NB300-605IR
FITC NB300-605F
HRP NB300-605H
Janelia Fluor 549 NB300-605JF549
Janelia Fluor 646 NB300-605JF646

Additional iNOS Products

Bioinformatics Tool for iNOS Antibody (NB300-605)

Discover related pathways, diseases and genes to iNOS Antibody (NB300-605). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for iNOS Antibody (NB300-605)

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Pathways for iNOS Antibody (NB300-605)

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Research Areas for iNOS Antibody (NB300-605)

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
09/15/2020
Application: WB
Species: Mouse

Verified Customer
11/13/2015
Application: IF
Species: Mouse

Leonid Tarassishin
04/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot