iNOS Antibody - BSA Free

Images

 
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours ...read more
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) ...read more
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Western blot image submitted by a verified customer review.
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - LCA-induced oxidative stress in 4T1 breast cancer cells. The 4T1 cells were treated with LCA for 48 h, then the indicated measurements were ...read more
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without ...read more
Indolepropionic acid (IPA) induced oxidative stress, cellular energy stress, and decreased the proportions of cancer stem cells. 500,000 cells/well 4T1 cells were treated with IPA in the concentrations indicated for 24 ...read more
Inhibitory effect of MMPP on amyloidogenesis and STAT3 translocation in astrocytes and microglia cells. The expression of APP, BACE1 and C99 was detected by Western blotting using specific antibodies in astrocytes (a) ...read more
miR-27a/b prevent pUbS65 accumulation upon mitochondrial damage. a, b Overexpression of PINK1 increased pUbS65 accumulation in HeLa cells only when incubated with CCCP. 48 h post-transfection, cells were incubated with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml
Validated by:
   

Biological Strategies

     

iNOS Antibody - BSA Free Summary

Immunogen
Sythetic peptide made to an internal portion of mouse iNOS (between amino acids 12-48) [UniProt P29477].
Specificity
This antibody detects iNOS. It does not detect other NOS isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (PMID 31536479)
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 35005642)
  • Immunohistochemistry-Paraffin 1:20
  • In vitro assay reported in scientific literature (PMID 27998907)
  • Western Blot 1:200 - 1:800
Application Notes
WB: Detects an approx. 135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. Also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples.
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
iNOS Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB300-605 in the following applications:

Publications
Read Publications using
NB300-605 in the following applications:

  • B/N
    1 publication
  • EM
    2 publications
  • 1 publication
  • IB
    1 publication
  • 14 publications
  • 15 publications
  • IHC
    2 publications
  • 2 publications
  • 16 publications
  • IP
    1 publication
  • WB
    43 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 31292486).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for iNOS Antibody - BSA Free

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2

Background

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for iNOS Antibody (NB300-605)(121)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 11 applications: B/N, EM, FLOW, IB, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
B/N
(1)
EM
(2)
FLOW
(1)
IB
(1)
ICC/IF
(14)
IF/IHC
(15)
IHC
(2)
IHC-Fr
(2)
IHC-P
(16)
IP
(1)
WB
(43)
All Applications
Filter By Species
Human
(15)
Mouse
(49)
Rat
(15)
Porcine
(2)
All Species
Showing Publications 1 - 10 of 121. Show All 121 Publications.
Publications using NB300-605 Applications Species
Janani G, Zhang L, Badylak S et al. Silk fibroin bioscaffold from Bombyx mori and Antheraea assamensis elicits a distinct host response and macrophage activation paradigm in vivo and in vitro Biomaterials Advances 2022-11-01 [PMID: 36502549] (ICC/IF, Mouse) ICC/IF Mouse
Parker A The Role of DOCK2 in Atherosclerosis Formation by Modulating Macrophage Activation and Function Thesis 2022-01-01
Haba D, Ohmiya T, Sekino M et al. Efficacy of wearable vibration dressings on full-thickness wound healing in a hyperglycemic rat model Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2023-11-11 [PMID: 37950849] (IHC-P, Rat) IHC-P Rat
Wu CY, Cilic A, Pak O et al. CEACAM6 as a Novel Therapeutic Target to Boost HO-1-mediated Antioxidant Defense in COPD American journal of respiratory and critical care medicine 2023-05-23 [PMID: 37219322]
Schwarcz S, Kovács P, Kovács T et al. The pro- and antineoplastic effects of deoxycholic acid in pancreatic adenocarcinoma cell models Molecular biology reports 2023-06-01 [PMID: 37145211] (WB, Human)

Details:
WB 1:1000
WB Human
Ren Y, Zhang S, Weeks J et al. Reduced Angiogenesis and Delayed Endochondral Ossification in CD163-/- Mice Highlights a Role of M2 Macrophages During Bone Fracture Repair Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2023-03-27 [PMID: 36970754]
Kwon O, Lee S Ishige okamurae Attenuates Neuroinflammation and Cognitive Deficits in Mice Intracerebroventricularly Injected with LPS via Regulating TLR-4/MyD88-Dependent Pathways Antioxidants 2022-12-29 [PMID: 36670940] (WB, Mouse) WB Mouse
Smit TTA Metabolic switch from glycolysis towards fatty acid oxidation in Schwann cells in response to high glucose Thesis (IB, Mouse) IB Mouse
Dravid A, Dhanabalan K, Agarwal S, Agarwal R Resolvin D1 loaded nanoliposomes promote M2 macrophage polarization and are effective in the treatment of Osteoarthritis Bioengineering & Translational Medicine Dec 16 2021 (Mouse) Mouse
Show All 121 Publications.

Reviews for iNOS Antibody (NB300-605) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-605:
Filter by Applications
WB
(2)
IF
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot iNOS NB300-605
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 09/15/2020
View

Summary

ApplicationWestern Blot
Sample TestedLiver tissue
SpeciesMouse
Lotqd213201
Immunofluorescence iNOS NB300-605
Enlarge
5
reviewed by:
Verified Customer
IF Mouse 11/13/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse aortic root
SpeciesMouse
Western Blot iNOS NB300-605
Enlarge
5
reviewed by:
Leonid Tarassishin
WB Human 04/28/2014
View

Summary

ApplicationWestern Blot
Sample Testedhuman astrocyte lysate
SpeciesHuman
LotNJ17385 1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for iNOS Antibody (NB300-605). (Showing 1 - 2 of 2 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. Would there be any interference from other iNOS isoforms on a Western blot or any other method for NB300-605?
    • NB300-605 will only recognize the iNOS isoform, so there should be no interference from eNOS, nNOS or bNOS.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional iNOS Products

Research Areas for iNOS Antibody (NB300-605)

Find related products by research area.

Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
09/15/2020
Application: WB
Species: Mouse

Verified Customer
11/13/2015
Application: IF
Species: Mouse

Leonid Tarassishin
04/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot