iNOS Antibody Summary
Synthetic peptide: DLKEEKDINNNVKKT, corresponding to amino acids 17-31 of Mouse iNOS.
This detects iNOS.It does not detect other NOS isoforms.
Ammonium sulfate precipitation
Test in a species/application not listed above to receive a full credit towards a future purchase.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS, 1 mg/ml BSA
0.05% Sodium Azide
Ammonium sulfate precipitation
- Western Blot 1:200 - 1:800
- Immunocytochemistry/Immunofluorescence 1:50
- Immunohistochemistry 1:20
- Immunohistochemistry-Paraffin 1:20
Immunofluorescence: use at a dilution of 1/50. Western Blot: use at a dilution of 1/200.Not tested in other applications.Optimal dilutions concentrations should be determined by the end user. By Western blot, this antibody detects an ~135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. By Western blot, this antibody also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples.
|Read Publications using
NB300-605 in the following applications:
Rabbit reactivity reported in scientific literature (PMID: 9892689). Xenopus reactivity reported in scientific literature (PMID: 15210449). Feline reactivity reported in scientific literature (PMID: 22154426). Avian reactivity reported in scientific literature (PMID: 25170849), Porcine reactivity reported in scientific literature (PMID:12023622), Fish reactivity reported in scientific literature (PMID: 22119937).
Alternate Names for iNOS Antibody
- EC 126.96.36.199
- Hepatocyte NOS
- HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
- Inducible NO synthase
- Inducible NOS
- nitric oxide synthase 2, inducible
- nitric oxide synthase 2A (inducible, hepatocytes)
- nitric oxide synthase, inducible
- nitric oxide synthase, macrophage
- NOS, type II
- NOS2ANOS type II
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
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FAQs for iNOS Antibody (NB300-605). (Showing 1 - 1 of 1 FAQs).
We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
- We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
Positive Control Lysate(s)
Additional iNOS Antibody Products
Related Products by Gene
, ENDOTHELIAL NITRIC OXIDE SYNTHASE
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Bioinformatics Tool for iNOS Antibody (NB300-605)
Discover related pathways, diseases and genes to iNOS Antibody (NB300-605). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on iNOS.
iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti... Read full blog post.