iNOS Antibody - BSA Free

Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.

Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.

Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.

Immunocytochemistry/Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a iNOS polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight 488 conjugated secondary antibody. iNOS staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Western blot image submitted by a verified customer review.

Western Blot: iNOS Antibody [NB300-605] - LCA-induced oxidative stress in 4T1 breast cancer cells. The 4T1 cells were treated with LCA for 48 h, then the indicated measurements were performed. The level of iNOS protein was detected by western blotting (n = 3). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/9/1255), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a iNOS polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight 488 conjugated secondary antibody. iNOS staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

iNOS Antibody - BSA Free Summary

• Immunogen: Sythetic peptide made to an internal portion of mouse iNOS (between amino acids 12-48) [UniProt P29477].
• Specificity: This antibody detects iNOS. It does not detect other NOS isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOS2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:20
  • In vitro assay
  • Western Blot 1:200 - 1:800
• Application Notes: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:35005642).WB: Detects an approx. 135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. Also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples. Use In vitro assay reported in scientific literature (PMID: 27998907). Use in FLOW reported in scientific literature (PMID: 31536479)..
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  iNOS Overexpression Lysate

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 31292486).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for iNOS Antibody - BSA Free

• EC 1.14.13.39
• Hepatocyte NOS
• HEP-NOS
• Inducible NO synthase
• Inducible NOS
• iNOS
• nitric oxide synthase 2, inducible
• nitric oxide synthase 2A (inducible, hepatocytes)
• nitric oxide synthase, inducible
• nitric oxide synthase, macrophage
• NOS type II
• NOS
• NOS2
• NOS2A
• Peptidyl-cysteine S-nitrosylase NOS2

Background

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for iNOS Antibody (NB300-605)(102)

Publications using NB300-605

• Lamorie-Foote K, Liu Q, Shkirkova K et al. Particulate matter exposure and chronic cerebral hypoperfusion promote oxidative stress and induce neuronal and oligodendrocyte apoptosis in male mice Journal of neuroscience research 2022-12-04 [PMID: 36464774] (IHC-P, Mouse)
• Fang Z, Hu Y, Dai J et al. CS12192, a Novel JAK3/JAK1/TBK1 Inhibitor, Synergistically Enhances the Anti-Inflammation Effect of Methotrexate in a Rat Model of Rheumatoid Arthritis International journal of molecular sciences 2022-11-02 [PMID: 36362183] (IHC-P, Rat)
• Eo H, Lee S, Kim SH et al. Petasites japonicus leaf extract inhibits Alzheimer's-like pathology through suppression of neuroinflammation Food & function 2022-10-17 [PMID: 36193798]
• Kang R, Gamdzyk M, Luo Y et al. Three Days Delayed Recanalization Improved Neurological Function in pMCAO Rats by Increasing M2 Microglia-Possible Involvement of the IL-4R/STAT6/PPAR gamma Pathway Translational stroke research 2022-07-22 [PMID: 35867328] (WB, Rat)
  
  Details:
  Dilution used 1:1001
• Zeng X, Chen B, Wang L et al. Chitosan@Puerarin hydrogel for accelerated wound healing in diabetic subjects by miR-29ab1 mediated inflammatory axis suppression Bioactive materials 2023-01-01 [PMID: 35600974] (IHC-P, Mouse)
• Zhu K, Wang Y, Sarlus H et al. Myeloid cell-specific topoisomerase 1 inhibition using DNA origami mitigates neuroinflammation EMBO reports 2022-05-20 [PMID: 35593064] (ICC/IF, Mouse)
• Park MC, Goughnour PC, Jun S et al. Two distinct receptor-binding domains of human glycyl-tRNA synthetase 1 displayed on extracellular vesicles activate M1 polarization and phagocytic bridging of macrophages to cancer cells Cancer letters 2022-07-28 [PMID: 35523311]
• Kuek LE, McMahon DB, Ma RZ et al. Cilia Stimulatory and Antibacterial Activities of T2R Bitter Taste Receptor Agonist Diphenhydramine: Insights into Repurposing Bitter Drugs for Nasal Infections Pharmaceuticals (Basel, Switzerland) 2022-04-06 [PMID: 35455449] (ICC/IF, Human)
• Lee DS, Kim JE P2X7 Receptor Augments LPS-Induced Nitrosative Stress by Regulating Nrf2 and GSH Levels in the Mouse Hippocampus Antioxidants (Basel, Switzerland) 2022-04-13 [PMID: 35453462] (WB, Mouse)
• LaRue MM, Parker S, Puccini J et al. Metabolic reprogramming of tumor-associated macrophages by collagen turnover promotes fibrosis in pancreatic cancer Proceedings of the National Academy of Sciences of the United States of America 2022-04-19 [PMID: 35412885]
• Saclier M, Angelini G, Bonfanti C et al. Selective ablation of Nfix in macrophages attenuates muscular dystrophy by inhibiting fibro-adipogenic progenitor-dependent fibrosis The Journal of pathology 2022-03-17 [PMID: 35297529] (ICC/IF, Mouse)
• Theruvath J, Menard M, Smith BAH et al. Anti-GD2 synergizes with CD47 blockade to mediate tumor eradication Nature medicine 2022-01-13 [PMID: 35027753] (IHC-P)
• Li Y, Acosta F, Quan Y et al. Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model STAR Protocols 2022-03-01 [PMID: 35005642] (IHC-Fr, Mouse)
• Zhou J, Zhang C, Yang X et al. Study on the effect of sevoflurane on the cognitive function of aged rats based on the activation of cortical microglia Ibrain 2021-12-01 (WB, Rat)
• Cao L, Wang S, Zhang L, Li J mPEG-b-P(Glu-co-Phe) nanoparticles increase gastric retention time and gastric ulcer treatment efficacy of 20(S)-ginsenoside Rg3 Biomedicine & Pharmacotherapy 2022-02-01 [PMID: 35062071] (IHC-P, Rat)
• Mou KJ, Shen KF, Li YL et al. Adenosine A2A Receptor in Bone Marrow-Derived Cells Mediated Macrophages M2 Polarization via PPAR gamma-P65 Pathway in Chronic Hypoperfusion Situation Frontiers in aging neuroscience 2022-01-03 [PMID: 35046793] (ICC/IF, IHC-P, Mouse)
• Sp N, Kang DY, Jo ES Et al. Pivotal Role of Iron Homeostasis in the Induction of Mitochondrial Apoptosis by 6-Gingerol Through PTEN Regulated PD-L1 Expression in Embryonic Cancer Cells Frontiers in oncology 2021-11-03 [PMID: 34804985] (WB, Human)
• Wang T, Zhu G, Qin L et al. Kininogen Nitric Oxide Signaling at Nearby Non-excited Acupoints After Long-term Stimulation JID Innov 2021-12-15 [PMID: 34909734]
• Manigat LC, Granade ME, Taori S Et al. Loss of Diacylglycerol Kinase alpha Enhances Macrophage Responsiveness Frontiers in immunology 2021-11-05 [PMID: 34804012] (WB, Mouse)
• Gredic M, Wu CY, Hadzic S et al. Myeloid-cell-specific deletion of inducible nitric oxide synthase protects against smoke-induced pulmonary hypertension in mice The European respiratory journal 2022-04-01 [PMID: 34475225] (IF/IHC, Human)
• Sp N, Kang DY, Kim HD et al. Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-kappa B Signaling in CCD-986Sk Skin Fibroblasts Life (Basel, Switzerland) 2021-05-10 [PMID: 34068523]
• Seo MG, Jo MJ, Hong NI et al. Anti-Inflammatory and Anti-Vascular Leakage Effects by Combination of Centella asiatica and Vitis vinifera L. Leaf Extracts Evidence-based complementary and alternative medicine : eCAM 2021-04-14 [PMID: 33936244]
• Misra S, Kilari S, Yang B et al. Anti Human CX3CR1 VHH Molecule Attenuates Venous Neointimal Hyperplasia of Arteriovenous Fistula in Mouse Model Journal of the American Society of Nephrology : JASN 2021-04-23 [PMID: 33893223]
• Zhang JQJ, Saravanabavan S, Cheng KM et al. Long-term dietary nitrate supplementation does not reduce renal cyst growth in experimental autosomal dominant polycystic kidney disease PloS one 2021-04-22 [PMID: 33886581] (WB, Mouse)
• Parker A The Role of DOCK2 in Atherosclerosis Formation by Modulating Macrophage Activation and Function Thesis 2022-01-01
• Zhou W, Lin Z, Xiong Y Et al. Dual-Targeted Nanoplatform Regulating the Bone Immune Microenvironment Enhances Fracture Healing ACS applied materials & interfaces 2021-11-19 [PMID: 34797653] (WB)
• Carey RM, Adappa ND, Palmer JN, Lee RJ Neuropeptide Y Reduces Nasal Epithelial T2R Bitter Taste Receptor-Stimulated Nitric Oxide Production Nutrients 2021-09-27 [PMID: 34684394] (Human)
• Yu H, Hou G, Cao J et al. Mangiferin Alleviates Mitochondrial ROS in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration via Suppression of NF-kappa B Signaling Pathway Oxidative Medicine and Cellular Longevity 2021-06-11 [PMID: 34234886]
• Jandova J, Snell J, Hua A et al. Topical hypochlorous acid (HOCl) blocks inflammatory gene expression and tumorigenic progression in UV-exposed SKH-1 high risk mouse skin Redox biology 2021-06-11 [PMID: 34144392] (IF/IHC, Mouse)
• Shao D, Li K, You M et al. Macrophage polarization by plasma sprayed ceria coatings on titanium-based implants: Cerium valence state matters iScience 2021-06-18 [PMID: 34142044] (Mouse)
• Mouton AJ, Flynn ER, Moak SP et al. Dimethyl fumarate preserves left ventricular infarct integrity following myocardial infarction via modulation of cardiac macrophage and fibroblast oxidative metabolism Journal of molecular and cellular cardiology 2021-05-21 [PMID: 34023353]
• Raisi A, Dezfoulian O, Davoodi F et al. Salvia miltiorrhiza hydroalcoholic extract inhibits postoperative peritoneal adhesions in rats BMC complementary medicine and therapies 2021-04-20 [PMID: 33879143] (IF/IHC, Rat)
• Yu, C The Application of Dialysis and Metabolomics to Preserve Heart Function During Ex Situ Heart Perfusion Thesis 2022-01-01 (WB, Porcine)
• Ma X, Zhang Z, Shen M et al. Changes of type II collagenase biomarkers on IL-1beta-induced rat articular chondrocytes Experimental and therapeutic medicine 2021-06-01 [PMID: 33850554] (WB, Rat)
• Ahmad A, Fauzia E, Kumar M et al. Gelatin-Coated Polycaprolactone Nanoparticle-Mediated Naringenin Delivery Rescue Human Mesenchymal Stem Cells from Oxygen Glucose Deprivation-Induced Inflammatory Stress ACS Biomater Sci Eng 2021-01-06 [PMID: 33405831]
• Sari Z, Miko E, Kovacs T et al. Indoxylsulfate, a Metabolite of the Microbiome, Has Cytostatic Effects in Breast Cancer via Activation of AHR and PXR Receptors and Induction of Oxidative Stress Cancers (Basel) 2020-10-10 [PMID: 33050543] (WB, Mouse)
• SAri, Z, Miko, E Et al. Indolepropionic Acid, a Metabolite of the Microbiome, Has Cytostatic Properties in Breast Cancer by Activating AHR and PXR Receptors and Inducing Oxidative Stress. Cancers (Basel) 2020-08-25 [PMID: 32854297] (WB, Mouse)
• Sutter CH, Rainwater HM, Sutter TR Contributions of Nitric Oxide to AHR-Ligand-Mediated Keratinocyte Differentiation Int J Mol Sci 2020-08-08 [PMID: 32784365]
• Veetil AT, Zou J, Henderson KW et al. DNA-based fluorescent probes of NOS2 activity in live brains Proc. Natl. Acad. Sci. U.S.A. 2020-06-30 [PMID: 32554491] (ICC/IF, Mouse)
• Kuai Y, Liu H, Liu D et al. An ultralow dose of the NADPH oxidase inhibitor diphenyleneiodonium (DPI) is an economical and effective therapeutic agent for the treatment of colitis-associated colorectal cancer Theranostics 2020-05-20 [PMID: 32550901] (ICC/IF, Mouse)
• Hozain S, Cottrell J CDllb+ targeted depletion of macrophages negatively affects bone fracture healing Bone 2020-06-12 [PMID: 32535290] (IHC-P, Mouse)
• Chang CY, Chen JY, Wu MH, Hu ML Therapeutic treatment with vitamin C reduces focal cerebral ischemia-induced brain infarction in rats by attenuating disruptions of blood brain barrier and cerebral neuronal apoptosis Free Radic. Biol. Med. 2020-05-23 [PMID: 32450129] (IF/IHC, Rat)
• Saclier M, Lapi M, Bonfanti C et Al. The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration Cells 2020-03-13 [PMID: 32183151]
• Corti P, Rochon E, Xue J et al. Cytoglobin regulates nitric oxide-dependent cilia motility and organ laterality in development Research Square 2022-10-11 (IHC-P, Mouse)
• Shao Q, Yang M, Liang C, et al. C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis Autophagy 2019-12-26 [PMID: 31847700] (ICC/IF, Mouse)
• Chow S K, Chim Y N et al. Vibration treatment modulates macrophage polarisation and enhances early inflammatory response in oestrogen-deficient osteoporotic-fracture healing. Eur Cell Mater 2019-07-11 [PMID: 31697398] (IF/IHC, Rat)
• Kaya S Protective effects of hesperetin on lipopolysaccharide-induced acute lung injury in a rat model Turk Gogus Kalp Dama 2020-04-20 [PMID: 30807747] (IF/IHC, IHC-P, Rat)
• Malhotra R, Wunderer F et al. Hepcidin Deficiency Protects Against Atherosclerosis. Arterioscler Thromb Vasc Biol 2019-01-02 [PMID: 30587002] (WB, Mouse)
• Fagan KA et al. Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension. Respir Res;2(5):306-13. 2001-01-01 [PMID: 11686901] (WB)
• Smit TTA Metabolic switch from glycolysis towards fatty acid oxidation in Schwann cells in response to high glucose Thesis (IB, Mouse)
• Kwon O, Lee S Ishige okamurae Attenuates Neuroinflammation and Cognitive Deficits in Mice Intracerebroventricularly Injected with LPS via Regulating TLR-4/MyD88-Dependent Pathways Antioxidants 2022-12-29 [PMID: 36670940] (WB, Mouse)
• Yu, C The Application of Dialysis and Metabolomics to Preserve Heart Function During Ex Situ Heart Perfusion Thesis 2022-01-01 (WB, Porcine)
• Liu B, Kong Y, Alimi OA et al. Multifunctional Microgel-Based Cream Hydrogels for Postoperative Abdominal Adhesion Prevention ACS nano 2023-02-28 [PMID: 36779870]
• Gredic M, Sharma V, Hadzic S et al. iNOS Deletion in Alveolar Epithelium Cannot Reverse the Elastase-Induced Emphysema in Mice Cells 2022-12-28 [PMID: 36611917] (IHC-P, Human, Mouse)
• SuSnjara P, Mihaljevic Z, Stupin A et al. Consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooygenases� metabolites in healthy young persons - A randomized study Research Square 2022-11-02 (WB, Human)
  
  Details:
  Dilutions: 1:500
• Vladimirova O, Soldan S, Su C et al. Elevated iNOS and 3'-nitrotyrosine in Kaposi's Sarcoma tumors and mouse model Tumour virus research 2023-03-01 [PMID: 36863485] (WB, Human)
• Dravid A, Dhanabalan K, Naskar S et al. Sustained release Resolvin D1 liposomes are effective in the treatment of osteoarthritis in obese mice bioRxiv 2023-01-23
• Janani G, Zhang L, Badylak S et al. Silk fibroin bioscaffold from Bombyx mori and Antheraea assamensis elicits a distinct host response and macrophage activation paradigm in vivo and in vitro Biomaterials Advances 2022-11-01 [PMID: 36502549] (ICC/IF, Mouse)
• Song BY, Chen C, Xu WH et al. Corticostriatal Projections Relying on GABA Levels Mediate Exercise-Induced Functional Recovery in Cerebral Ischemic Mice Vascular health and risk management 2022-12-02 [PMID: 36483534] (WB, Human)
• Do DC, Mu J, Ke X et al. miR-511-3p protects against cockroach allergen-induced lung inflammation by antagonizing CCL2 JCI Insight 2019-09-19 [PMID: 31536479] (FLOW, Mouse)
• Karaboga I Caffeic acid phenethyl ester ameliorates pulmonary inflammation and apoptosis reducing Nf- kappa B activation in blunt pulmonary contusion model Ulus Travma Acil Cerrahi Derg 2019-08-01 [PMID: 31475327]
• Marek-Iannucci S, Thomas A, Hou J et al. Myocardial hypothermia increases autophagic flux, mitochondrial mass and myocardial function after ischemia-reperfusion injury Sci Rep 2019-07-10 [PMID: 31292486] (IHC-P, Porcine)
• Singh, K;Gobert, AP;Coburn, LA;Barry, DP;Allaman, M;Asim, M;Luis, PB;Schneider, C;Milne, GL;Boone, HH;Shilts, MH;Washington, MK;Das, SR;Piazuelo, MB;Wilson, KT; Dietary Arginine Regulates Severity of Experimental Colitis and Affects the Colonic Microbiome Front Cell Infect Microbiol 2019-03-26 [PMID: 30972302] (ICC/IF, Mouse)
• Wang CN, Lin YC, Chang BC et al. Targeting the phosphorylation site of MARCKS alleviates symptoms of steroid-resistant like asthma in a murine model Br. J. Pharmacol. 2019-02-01 [PMID: 30706455] (WB, Mouse)
• Zhang H, Wei Q, Xiang X et al. Semaphorin 4A acts in a feed-forward loop with NF-kB pathway to exacerbate catabolic effect of IL-1b on chondrocytes. Int. Immunopharmacol. 2019-01-24 [PMID: 30685700] (WB, Mouse)
• Yu Y, Park SJ, Beyak MJ. Inducible nitric oxide synthase-derived nitric oxide reduces vagal satiety signalling in obese mice J. Physiol. (Lond.) 2018-12-18 [PMID: 30565225] (WB, Mouse)
• Zhang X, Zhao Y, Zhu X et al. Active vitamin D regulates macrophage M1/M2 phenotypes via the STAT-1-TREM-1 pathway in diabetic nephropathy J. Cell. Physiol. 2018-11-27 [PMID: 30478987] (ICC/IF, Human)
• Liu W, Li J, Cheng M et al. Zinc-Modified Sulfonated Polyetheretherketone Surface with Immunomodulatory Function for Guiding Cell Fate and Bone Regeneration Adv. Sci. 2018-08-07 [PMID: 30356934] (IF/IHC, Mouse)
• Zhu J, He J, Verano M et al. An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation. Lab Chip. 2018-10-10 [PMID: 30302487] (ICC/IF, Mouse)
• Lee S, Kim GL, Kim NY et al. ATF3 Stimulates IL-17A by Regulating Intracellular Ca2+/ROS-Dependent IL-1B Activation During Streptococcus pneumoniae Infection. Front Immunol 2018-08-30 [PMID: 30214444] (WB, Mouse)
• Choi JY, Yeo IJ, Kim KC et al. K284-6111 prevents the amyloid beta-induced neuroinflammation and impairment of recognition memory through inhibition of NF-kB-mediated CHI3L1 expression J Neuroinflammation 2018-08-11 [PMID: 30098604] (WB, IF/IHC, Mouse)
• Shaalan A, Carpenter G, Proctor G. Inducible nitric oxide synthase-mediated injury in a mouse model of acute salivary gland dysfunction. Nitric Oxide 2018-06-07 [PMID: 29885902] (WB, Mouse)
• Hao Yanli, Niu Hao, An Shuhong et al. Downregulation of iNOS, IL-1B, and P2X7 Expression in Mast Cells via Activation of PAR4 Contributes to the Inhibition of Visceral Hyperalgesia in Rats. Journal of Immunology Research 2018-01-01 [PMID: 29854833] (WB, Rat)
• Gu Sun Mi, Lee Hee Pom, Ham Young Wan et al. Piperlongumine Improves Lipopolysaccharide-Induced Amyloidogenesis by Suppressing NF-KappaB Pathway. Neuromolecular Medicine 2018-07-06 [PMID: 29802525] (WB, Mouse)
• Wang CC, Wee HY, Hu CY, Chio CC. The Effects of Memantine on Glutamic Receptor-Associated Nitrosative Stress in a Traumatic Brain Injury Rat Model. World Neurosurg. 2018-01-31 [PMID: 29382619] (Rat)
• Shaalan A, Carpenter G, Proctor G. Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo. Mol Immunol 2018-02-01 [PMID: 29331803] (Mouse)
• Choi JY, Jang JS, Son DJ et al. Antarctic Krill Oil Diet Protects against Lipopolysaccharide-Induced Oxidative Stress, Neuroinflammation and Cognitive Impairment. Int J Mol Sci 2017-11-28 [PMID: 29182579] (Mouse)
• Choi JY, Hwang CJ, Lee DY et al. (E)-2-Methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) Phenol Ameliorates LPS-Mediated Memory Impairment by Inhibition of STAT3 Pathway.. Neuromolecular Med. 2017-10-19 [PMID: 29052076] (Mouse)
• Masola V, Zaza G, Bellin G et al. Heparanase regulates the M1 polarization of renal macrophages and their crosstalk with renal epithelial tubular cells after ischemia/reperfusion injury. FASEB J. 2017-09-28 [PMID: 28970256] (ICC/IF, Mouse)
• Moriwaki K, Asahi M. Augmented TME O-GlcNAcylation Promotes Tumor Proliferation through the Inhibition of p38 MAPK. Mol. Cancer Res. 2017-05-23 [PMID: 28536142] (WB, Mouse)
• Hariri BM, McMahon DB, Chen B et al. Flavones modulate respiratory epithelial innate immunity: anti-inflammatory effects and activation of T2R14. J. Biol. Chem. 2017-04-03 [PMID: 28373278] (ICC/IF, Human)
• Waniczek D, Lorenc Z, Snietura M et al. Tumor-Associated Macrophages and Regulatory T Cells Infiltration and the Clinical Outcome in Colorectal Cancer. Arch. Immunol. Ther. Exp. (Warsz.). 2017-03-25 [PMID: 28343267] (IHC-P, Human)
• Kim YE, Hwang CJ, Lee HP et al. Inhibitory effect of punicalagin on lipopolysaccharide-induced neuroinflammation, oxidative stress and memory impairment via inhibition of nuclear factor-kappaB Neuropharmacology 2017-01-26 [PMID: 28132781] (WB, IF/IHC, Mouse)
• Ridiandries A, Tan JT, Ravindran D et al. CC-chemokine class inhibition attenuates pathological angiogenesis while preserving physiological angiogenesis. FASEB J 2017-03-01 [PMID: 27998907]
• Humbert-Claude M, Duc D, Dwir D et al. Tollip, an early regulator of the acute inflammatory response in the substantia nigra. J Neuroinflammation. 2016-12-07 [PMID: 27927222] (IF/IHC, Mouse)
• Li M, Qian S, . Gastrodin Protects Neural Progenitor Cells Against Amyloid beta (1-42)-Induced Neurotoxicity and Improves Hippocampal Neurogenesis in Amyloid beta (1-42)-Injected Mice. J Mol Neurosci 2016-09-01 [PMID: 27112440]
• Wezel A, de Vries MR, Maassen JM et al. Deficiency of the TLR4 analogue RP105 aggravates vein graft disease by inducing a pro-inflammatory response. Sci Rep. 2016-04-07 [PMID: 27053419] (IHC-P, Mouse)
• Kim HJ, Yoo HY, Jang JH et al. Wall stretch and thromboxane A2 activate NO synthase (eNOS) in pulmonary arterial smooth muscle cells via H2O2 and Akt-dependent phosphorylation. Pflugers Arch. 2016-01-04 [PMID: 26729266] (IHC-P, Rat)
• Yun Jaesuk, Gu Sun Mi, Yun Hyung Mun et al. Myelin oligodendrocyte glycoprotein (MOG35-55)-induced experimental autoimmune encephalomyelitis is amelioRated in interleukin-32 alpha transgenic mice. Oncotarget 2015-01-01 [PMID: 26564962] (IF/IHC, WB, Mouse)
• Kober C, Rohn S, Weibel S et al. Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus traps. J Transl Med 2015-01-01 [PMID: 26149494] (WB)
• Costabile G, d'Angelo I, Rampioni G. Toward Repositioning Niclosamide for Antivirulence Therapy of Pseudomonas aeruginosa Lung Infections: Development of Inhalable Formulations through Nanosuspension Technology. Molecular Pharmaceutics. 2015-05-14 [PMID: 25974285] (WB, Rat)
• Tarassishin L, Suh HS, Lee SC et al. LPS and IL-1 differentially activate mouse and human astrocytes: role of CD14. Glia 2014-06-01 [PMID: 24659539] (WB, Mouse)
• Han JA, Seo EY, Kim HJ et al. Hypoxia-augmented constriction of deep femoral artery mediated by inhibition of eNOS in smooth muscle Am J Physiol Cell Physiol 2012-10-24 [PMID: 23099643] (WB, IF/IHC, Rat)
• Liao PH, Hsu YH, Yang HH et al. Involvement of extraneural tissues and upregulation of inducible nitric oxide synthase after experimental infection with rabies virus in BALB/c mice and LEW/SsN rats Pathol Int 2012-09-01 [PMID: 22924848] (IF/IHC, Mouse)
• Wang H, Peters T et al. Activated macrophages are essential in a murine model for T cell-mediated chronic psoriasiform skin inflammation. J Clin Invest;116(8):2105-14. 2006-08-01 [PMID: 16886059] (IF/IHC)
• Sakai K, Suzuki H et al. Phosphoinositide 3-kinase in nitric oxide synthesis in macrophage: critical dimerization of inducible nitric-oxide synthase. J Biol Chem;281(26):17736-42. 2006-06-30 [PMID: 16636057] (WB)
• Adler A, Huang H et al. Endocardial endothelium in the avascular frog heart: role for diffusion of NO in control of cardiac O2 consumption. Am J Physiol Heart Circ Physiol;287(1):H14-21. 2004-07-01 [PMID: 15210449] (WB)
• Ziolo MT, Maier et al. Myocyte nitric oxide synthase 2 contributes to blunted beta-adrenergic response in failing human hearts by decreasing Ca2+ transients. Circulation;109(15):1886-91. 2004-04-20 [PMID: 15037528] (WB)
• Pulido EJ, Shames BD et al. Differential inducible nitric oxide synthase expression in systemic and pulmonary vessels after endotoxin. Am J Physiol Regul Integr Comp Physiol;278(5):R1232-9. 2000-05-01 [PMID: 10801292] (WB, IF/IHC)
• Xu KY, Huso DL et al. Nitric oxide synthase in cardiac sarcoplasmic reticulum. Proc Natl Acad Sci U S A. 96(2):657-62. 1999-01-19 [PMID: 9892689] (WB)
• Bredt DS, Hwang PM et al. Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. Nature;351(6329):714-8. 1991-06-27 [PMID: 1712077] (WB)

Reviews for iNOS Antibody (NB300-605) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Mouse.

reviewed by: Zeyuan Zhang WB Mouse 09/15/2020

Summary

• Application: Western Blot
• Sample Tested: Liver tissue
• Species: Mouse
• Lot: qd213201

reviewed by: Dina Schneider IF Mouse 11/13/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse aortic root
• Species: Mouse

reviewed by: Verified Customer WB Human 04/28/2014

Summary

• Application: Western Blot
• Sample Tested: human astrocyte lysate
• Species: Human
• Lot: NJ17385 1

FAQs for iNOS Antibody (NB300-605). (Showing 1 - 2 of 2 FAQs).

1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
   • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
2. Would there be any interference from other iNOS isoforms on a Western blot or any other method for NB300-605?
   • NB300-605 will only recognize the iNOS isoform, so there should be no interference from eNOS, nNOS or bNOS.

Bioinformatics

• Gene Symbol: NOS2
• Entrez:
  • 4843(Human)
  • 18126(Mouse)
  • 24599(Rat)
• Uniprot:
  • P29477(Mouse)