iNOS Antibody

Images

 
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours ...read more
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) ...read more
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Validated by:
   

Biological Strategies

     

iNOS Antibody Summary

Immunogen
iNOS Antibody was made to a synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.
Specificity
This antibody detects iNOS. It does not detect other NOS isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200 - 1:800
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:20
  • In vitro assay
Application Notes
WB: Detects an approx. 135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. Also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples. Use In vitro assay reported in scientific literature (PMID: 27998907). Use in FLOW reported in scientific literature (PMID: 31536479).
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
iNOS Overexpression Lysate
iNOS Knockout HeLa Cell Lysate
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB300-605 in the following applications:

Publications
Read Publications using
NB300-605 in the following applications:

  • 1 publication
  • IB
    1 publication
  • 9 publications
  • IHC
    11 publications
  • 6 publications
  • WB
    22 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 31292486).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for iNOS Antibody

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2

Background

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-605
Species: Hu, Mu, Rt, Po
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro

Publications for iNOS Antibody (NB300-605)(53)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: Flow, IB, ICC/IF, IHC, IHC-P, WB.


Filter By Application
Flow
(1)
IB
(1)
ICC/IF
(9)
IHC
(11)
IHC-P
(6)
WB
(22)
All Applications
Filter By Species
Human
(4)
Mouse
(27)
Rat
(7)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using NB300-605 Applications Species
Smit TTA Metabolic switch from glycolysis towards fatty acid oxidation in Schwann cells in response to high glucose Thesis (IB, Mouse) IB Mouse
Sutter CH, Rainwater HM, Sutter TR Contributions of Nitric Oxide to AHR-Ligand-Mediated Keratinocyte Differentiation Int J Mol Sci Aug 8 2020 [PMID: 32784365]
Veetil AT, Zou J, Henderson KW et al. DNA-based fluorescent probes of NOS2 activity in live brains Proc. Natl. Acad. Sci. U.S.A. Jun 30 2020 [PMID: 32554491] (ICC/IF, Mouse) ICC/IF Mouse
Kuai Y, Liu H, Liu D et al. An ultralow dose of the NADPH oxidase inhibitor diphenyleneiodonium (DPI) is an economical and effective therapeutic agent for the treatment of colitis-associated colorectal cancer Theranostics May 20 2020 [PMID: 32550901] (ICC/IF, Mouse) ICC/IF Mouse
Hozain S, Cottrell J CDllb+ targeted depletion of macrophages negatively affects bone fracture healing Bone Jun 12 2020 [PMID: 32535290] (IHC-P, Mouse) IHC-P Mouse
Chang CY, Chen JY, Wu MH, Hu ML Therapeutic treatment with vitamin C reduces focal cerebral ischemia-induced brain infarction in rats by attenuating disruptions of blood brain barrier and cerebral neuronal apoptosis Free Radic. Biol. Med. May 23 2020 [PMID: 32450129] (IHC, Rat) IHC Rat
Saclier M, Lapi M, Bonfanti C et Al. The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration Cells Mar 13 2020 [PMID: 32183151]
Shao Q, Yang M, Liang C, et al. C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis Autophagy Dec 26 2019 [PMID: 31847700] (ICC/IF, Mouse) ICC/IF Mouse
Do DC, Mu J, Ke X et al. miR-511-3p protects against cockroach allergen-induced lung inflammation by antagonizing CCL2 JCI Insight Sep 19 2019 [PMID: 31536479] (Flow, Mouse) Flow Mouse
Karaboga I Caffeic acid phenethyl ester ameliorates pulmonary inflammation and apoptosis reducing Nf- kappa B activation in blunt pulmonary contusion model Ulus Travma Acil Cerrahi Derg Aug 1 2019 [PMID: 31475327]
Show All 53 Publications.

Reviews for iNOS Antibody (NB300-605) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-605:
Filter by Applications
WB
(2)
IF
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot iNOS NB300-605
Enlarge
4
reviewed by:
Anonymous
WB Mouse 09/15/2020
View

Summary

ApplicationWestern Blot
Sample TestedLiver tissue
SpeciesMouse
Lotqd213201
Immunofluorescence iNOS NB300-605
Enlarge
5
reviewed by:
Anonymous
IF Mouse 11/13/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse aortic root
SpeciesMouse

Blocking

Blocking Details30 minutes in normal donkey serum/TBST room temperature

Details

Detection NotesDetected Rhodamine Red from secondary antibody using fluorescence microscope
Fixation DetailsParaffin
Western Blot iNOS NB300-605
Enlarge
5
reviewed by:
Leonid Tarassishin
WB Human 04/28/2014
View

Summary

ApplicationWestern Blot
Sample Testedhuman astrocyte lysate
SpeciesHuman
LotNJ17385 1
Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)

Blocking

Blocking Details5% non-fat milk in PBS

Primary Anitbody

Dilution Ratio1:250, 18 hr, 4C, PBST (PBS+0.05%Tween 20)

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#Pierce/ThermoScientific, Cat. # 32460
Secondary Concentration1:500 (stock 10 ug/ml)

Details

Detection NotesECL-Pico (Piere/ThermoScientific), 1 min; Untreated and treated with IL-1beta cells; Loading Ctr: beta-Actin.

Comments

Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for iNOS Antibody (NB300-605). (Showing 1 - 2 of 2 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. Would there be any interference from other iNOS isoforms on a Western blot or any other method for NB300-605?
    • NB300-605 will only recognize the iNOS isoform, so there should be no interference from eNOS, nNOS or bNOS.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB300-605AF405
Alexa Fluor 488 NB300-605AF488
Alexa Fluor 647 NB300-605AF647
Alexa Fluor 700 NB300-605AF700
Biotin NB300-605B
DyLight 350 NB300-605UV
DyLight 405 NB300-605V
DyLight 488 NB300-605G
DyLight 550 NB300-605R
DyLight 594 NB300-605DL594
DyLight 650 NB300-605C
DyLight 680 NB300-605FR
DyLight 755 NB300-605IR
FITC NB300-605F
HRP NB300-605H
Janelia Fluor 549 NB300-605JF549
Janelia Fluor 646 NB300-605JF646

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Bioinformatics Tool for iNOS Antibody (NB300-605)

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Diseases for iNOS Antibody (NB300-605)

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Pathways for iNOS Antibody (NB300-605)

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Research Areas for iNOS Antibody (NB300-605)

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
09/15/2020
Application: WB
Species: Mouse

Anonymous
11/13/2015
Application: IF
Species: Mouse

Leonid Tarassishin
04/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot