iNOS Antibody


Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours more
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) more
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Western blot image submitted by a verified customer review.
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - LCA-induced oxidative stress in 4T1 breast cancer cells. The 4T1 cells were treated with LCA for 48 h, then the indicated measurements were more
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without more

Product Details

Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro
0.5 mg/ml
Validated by:

Biological Strategies


iNOS Antibody Summary

iNOS Antibody was made to a synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.
This antibody detects iNOS. It does not detect other NOS isoforms.
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:20
  • In vitro assay
  • Western Blot 1:200 - 1:800
Application Notes
WB: Detects an approx. 135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. Also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples. Use In vitro assay reported in scientific literature (PMID: 27998907). Use in FLOW reported in scientific literature (PMID: 31536479).
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
iNOS Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.7
NB300-605 in the following applications:

Read Publications using
NB300-605 in the following applications:

  • 1 publication
  • IB
    1 publication
  • 9 publications
  • IHC
    14 publications
  • 6 publications
  • WB
    26 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 31292486).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
0.5 mg/ml
Affinity purified

Alternate Names for iNOS Antibody

  • EC
  • Hepatocyte NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2


Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).


1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for iNOS Antibody (NB300-605)(69)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: Flow, IB, ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 69. Show All 69 Publications.
Publications using NB300-605 Applications Species
Yu H, Hou G, Cao J et al. Mangiferin Alleviates Mitochondrial ROS in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration via Suppression of NF-kappa B Signaling Pathway Oxidative Medicine and Cellular Longevity Jun 11 2021 [PMID: 34234886]
Jandova J, Snell J, Hua A et al. Topical hypochlorous acid (HOCl) blocks inflammatory gene expression and tumorigenic progression in UV-exposed SKH-1 high risk mouse skin Redox biology Jun 11 2021 [PMID: 34144392] (IHC, Mouse) IHC Mouse
Li Y, Li Z, Quan Y et al. Macrophage recruitment in immune-privileged lens during capsule repair, necrotic fiber removal, and fibrosis iScience Jun 1 2021 [PMID: 34142044] (Mouse) Mouse
Sp N, Kang DY, Kim HD et al. Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-kappa B Signaling in CCD-986Sk Skin Fibroblasts Life (Basel, Switzerland) May 10 2021 [PMID: 34068523]
Mouton AJ, Flynn ER, Moak SP et al. Dimethyl fumarate preserves left ventricular infarct integrity following myocardial infarction via modulation of cardiac macrophage and fibroblast oxidative metabolism Journal of molecular and cellular cardiology May 21 2021 [PMID: 34023353]
Seo MG, Jo MJ, Hong NI et al. Anti-Inflammatory and Anti-Vascular Leakage Effects by Combination of Centella asiatica and Vitis vinifera L. Leaf Extracts Evidence-based complementary and alternative medicine : eCAM Apr 14 2021 [PMID: 33936244]
Misra S, Kilari S, Yang B et al. Anti Human CX3CR1 VHH Molecule Attenuates Venous Neointimal Hyperplasia of Arteriovenous Fistula in Mouse Model Journal of the American Society of Nephrology : JASN Apr 23 2021 [PMID: 33893223]
Zhang JQJ, Saravanabavan S, Cheng KM et al. Long-term dietary nitrate supplementation does not reduce renal cyst growth in experimental autosomal dominant polycystic kidney disease PloS one Apr 22 2021 [PMID: 33886581] (WB, Mouse) WB Mouse
Raisi A, Dezfoulian O, Davoodi F et al. Salvia miltiorrhiza hydroalcoholic extract inhibits postoperative peritoneal adhesions in rats BMC complementary medicine and therapies Apr 20 2021 [PMID: 33879143] (IHC, Rat) IHC Rat
Ma X, Zhang Z, Shen M et al. Changes of type II collagenase biomarkers on IL-1beta-induced rat articular chondrocytes Experimental and therapeutic medicine Jun 1 2021 [PMID: 33850554] (WB, Rat) WB Rat
Show All 69 Publications.

Reviews for iNOS Antibody (NB300-605) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-605:
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Images Ratings Applications Species Date Details
Western Blot iNOS NB300-605
reviewed by:
Verified Customer
WB Mouse 09/15/2020


ApplicationWestern Blot
Sample TestedLiver tissue
Immunofluorescence iNOS NB300-605
reviewed by:
Verified Customer
IF Mouse 11/13/2015


Sample TestedMouse aortic root


Blocking Details30 minutes in normal donkey serum/TBST room temperature


Detection NotesDetected Rhodamine Red from secondary antibody using fluorescence microscope
Fixation DetailsParaffin
Western Blot iNOS NB300-605
reviewed by:
Leonid Tarassishin
WB Human 04/28/2014


ApplicationWestern Blot
Sample Testedhuman astrocyte lysate
LotNJ17385 1
Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)


Blocking Details5% non-fat milk in PBS

Primary Anitbody

Dilution Ratio1:250, 18 hr, 4C, PBST (PBS+0.05%Tween 20)

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#Pierce/ThermoScientific, Cat. # 32460
Secondary Concentration1:500 (stock 10 ug/ml)


Detection NotesECL-Pico (Piere/ThermoScientific), 1 min; Untreated and treated with IL-1beta cells; Loading Ctr: beta-Actin.


Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for iNOS Antibody (NB300-605). (Showing 1 - 2 of 2 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. Would there be any interference from other iNOS isoforms on a Western blot or any other method for NB300-605?
    • NB300-605 will only recognize the iNOS isoform, so there should be no interference from eNOS, nNOS or bNOS.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 NB300-605AF405
Alexa Fluor 488 NB300-605AF488
Alexa Fluor 647 NB300-605AF647
Alexa Fluor 700 NB300-605AF700
Biotin NB300-605B
DyLight 350 NB300-605UV
DyLight 405 NB300-605V
DyLight 488 NB300-605G
DyLight 550 NB300-605R
DyLight 594 NB300-605DL594
DyLight 650 NB300-605C
DyLight 680 NB300-605FR
DyLight 755 NB300-605IR
FITC NB300-605F
HRP NB300-605H
Janelia Fluor 549 NB300-605JF549
Janelia Fluor 646 NB300-605JF646

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Mouse

Verified Customer
Application: IF
Species: Mouse

Leonid Tarassishin
Application: WB
Species: Human


Gene Symbol NOS2