iNOS Antibody


Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours more
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) more
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without more

Product Details

Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro
0.5 mg/ml
Validated by:

Biological Strategies


iNOS Antibody Summary

iNOS Antibody was made to a synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.
This antibody detects iNOS. It does not detect other NOS isoforms.
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:200 - 1:800
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:20
  • In vitro assay
Application Notes
WB: Detects an approx. 135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. Also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples. Use In vitro assay reported in scientific literature (PMID: 27998907). Use in FLOW reported in scientific literature (PMID: 31536479).
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
iNOS Overexpression Lysate
iNOS Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
NB300-605 in the following applications:

Read Publications using
NB300-605 in the following applications:

  • 1 publication
  • IB
    1 publication
  • 7 publications
  • IHC
    11 publications
  • 5 publications
  • WB
    22 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 31292486).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
0.5 mg/ml
Affinity purified

Alternate Names for iNOS Antibody

  • EC
  • Hepatocyte NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2


Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).


1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for iNOS Antibody (NB300-605)(49)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: Flow, IB, ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using NB300-605 Applications Species
Chang CY, Chen JY, Wu MH, Hu ML Therapeutic treatment with vitamin C reduces focal cerebral ischemia-induced brain infarction in rats by attenuating disruptions of blood brain barrier and cerebral neuronal apoptosis Free Radic. Biol. Med. May 23 2020 [PMID: 32450129] (IHC, Rat) IHC Rat
Saclier M, Lapi M, Bonfanti C et Al. The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration Cells Mar 13 2020 [PMID: 32183151]
Shao Q, Yang M, Liang C, et al. C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis Autophagy Dec 26 2019 [PMID: 31847700] (ICC/IF, Mouse) ICC/IF Mouse
Do DC, Mu J, Ke X et al. miR-511-3p protects against cockroach allergen-induced lung inflammation by antagonizing CCL2 JCI Insight Sep 19 2019 [PMID: 31536479] (Flow, Mouse) Flow Mouse
Karaboga I Caffeic acid phenethyl ester ameliorates pulmonary inflammation and apoptosis reducing Nf- kappa B activation in blunt pulmonary contusion model Ulus Travma Acil Cerrahi Derg Aug 1 2019 [PMID: 31475327]
Marek-Iannucci S, Thomas A, Hou J et al. Myocardial hypothermia increases autophagic flux, mitochondrial mass and myocardial function after ischemia-reperfusion injury Sci Rep Jul 10 2019 [PMID: 31292486] (IHC-P, Porcine) IHC-P Porcine
Singh, K;Gobert, AP;Coburn, LA;Barry, DP;Allaman, M;Asim, M;Luis, PB;Schneider, C;Milne, GL;Boone, HH;Shilts, MH;Washington, MK;Das, SR;Piazuelo, MB;Wilson, KT; Dietary Arginine Regulates Severity of Experimental Colitis and Affects the Colonic Microbiome Front Cell Infect Microbiol Mar 26 2019 [PMID: 30972302] (ICC/IF, Mouse) ICC/IF Mouse
Kaya S Protective effects of hesperetin on lipopolysaccharide-induced acute lung injury in a rat model Turk Gogus Kalp Dama Apr 20 2020 [PMID: 30807747] (IHC-P, IHC, Rat) IHC-P, IHC Rat
Wang CN, Lin YC, Chang BC et al. Targeting the phosphorylation site of MARCKS alleviates symptoms of steroid-resistant like asthma in a murine model Br. J. Pharmacol. Feb 1 2019 [PMID: 30706455] (WB, Mouse) WB Mouse
Zhang H, Wei Q, Xiang X et al. Semaphorin 4A acts in a feed-forward loop with NF-kB pathway to exacerbate catabolic effect of IL-1b on chondrocytes. Int. Immunopharmacol. Jan 24 2019 [PMID: 30685700] (WB, Mouse) WB Mouse
Show All 49 Publications.

Reviews for iNOS Antibody (NB300-605) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-605:
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Images Ratings Applications Species Date Details
Immunofluorescence iNOS NB300-605
reviewed by:
IF Mouse 11/13/2015


Sample TestedMouse aortic root


Blocking Details30 minutes in normal donkey serum/TBST room temperature


Detection NotesDetected Rhodamine Red from secondary antibody using fluorescence microscope
Fixation DetailsParaffin
Western Blot iNOS NB300-605
reviewed by:
Leonid Tarassishin
WB Human 04/28/2014


ApplicationWestern Blot
Sample Testedhuman astrocyte lysate
LotNJ17385 1
Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)


Blocking Details5% non-fat milk in PBS

Primary Anitbody

Dilution Ratio1:250, 18 hr, 4C, PBST (PBS+0.05%Tween 20)

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#Pierce/ThermoScientific, Cat. # 32460
Secondary Concentration1:500 (stock 10 ug/ml)


Detection NotesECL-Pico (Piere/ThermoScientific), 1 min; Untreated and treated with IL-1beta cells; Loading Ctr: beta-Actin.


Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for iNOS Antibody (NB300-605). (Showing 1 - 2 of 2 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. Would there be any interference from other iNOS isoforms on a Western blot or any other method for NB300-605?
    • NB300-605 will only recognize the iNOS isoform, so there should be no interference from eNOS, nNOS or bNOS.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 NB300-605AF405
Alexa Fluor 488 NB300-605AF488
Alexa Fluor 647 NB300-605AF647
Alexa Fluor 700 NB300-605AF700
Biotin NB300-605B
DyLight 350 NB300-605UV
DyLight 405 NB300-605V
DyLight 488 NB300-605G
DyLight 550 NB300-605R
DyLight 594 NB300-605DL594
DyLight 650 NB300-605C
DyLight 680 NB300-605FR
DyLight 755 NB300-605IR
FITC NB300-605F
HRP NB300-605H
Janelia Fluor 549 NB300-605JF549
Janelia Fluor 646 NB300-605JF646

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews


Application: IF
Species: Mouse

Leonid Tarassishin
Application: WB
Species: Human


Gene Symbol NOS2