iNOS Antibody (NB300-605)

Images

 
Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours (right lane) and 10ul of PageRuler ...read more
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without ...read more
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Both normal and cancer biopsies of deparaffinized human Lung tissue.
Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) ...read more
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Both normal and cancer biopsies of deparaffinized human Heart tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
4 mg/ml

Order Details

iNOS Antibody Summary

Immunogen
Synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.
Specificity
This detects iNOS. It does not detect other NOS isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS2
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200 - 1:800
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:20
Application Notes
WB: Detects an approx. 135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. Also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples.
Positive Control
iNOS Lysate (NBL1-13721)
Reviewed Applications
Read 2 Reviews rated 5
using
NB300-605 in the following applications:

Publications
Read Publications using
NB300-605 in the following applications:

  • 1 publication
  • IHC
    6 publications
  • 3 publications
  • WB
    13 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
4 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for iNOS Antibody

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS
  • NOS, type II
  • NOS2
  • NOS2A
  • NOS2ANOS type II

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for iNOS Antibody (NB300-605)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(6)
IHC-P
(3)
WB
(13)
All Applications
Filter By Species
Human
(2)
Mouse
(6)
Rat
(3)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB300-605 Applications Species
Moriwaki K, Asahi M. Augmented TME O-GlcNAcylation Promotes Tumor Proliferation through the Inhibition of p38 MAPK. Mol. Cancer Res. May 23 2017 [PMID: 28536142] (WB, Mouse) WB Mouse
Hariri BM, McMahon DB, Chen B et al. Flavones modulate respiratory epithelial innate immunity: anti-inflammatory effects and activation of T2R14. J. Biol. Chem. Apr 3 2017 [PMID: 28373278] (ICC/IF, Human) ICC/IF Human
Waniczek D, Lorenc Z, Snietura M et al. Tumor-Associated Macrophages and Regulatory T Cells Infiltration and the Clinical Outcome in Colorectal Cancer. Arch. Immunol. Ther. Exp. (Warsz.). Mar 25 2017 [PMID: 28343267] (IHC-P, Human) IHC-P Human
Kim YE, Hwang CJ, Lee HP et al. Inhibitory effect of punicalagin on lipopolysaccharide-induced neuroinflammation, oxidative stress and memory impairment via inhibition of nuclear factor-kappaB Neuropharmacology Jan 26 2017 [PMID: 28132781] (IHC, WB, Mouse) IHC, WB Mouse
Ridiandries A, Tan JT, Ravindran D et al. CC-chemokine class inhibition attenuates pathological angiogenesis while preserving physiological angiogenesis. FASEB J. Dec 20 2016 [PMID: 27998907]
Humbert-Claude M, Duc D, Dwir D et al. Tollip, an early regulator of the acute inflammatory response in the substantia nigra. J Neuroinflammation. Dec 7 2016 [PMID: 27927222] (IHC, Mouse) IHC Mouse
Li M, Qian S,. Gastrodin Protects Neural Progenitor Cells Against Amyloid beta (1-42)-Induced Neurotoxicity and Improves Hippocampal Neurogenesis in Amyloid beta (1-42)-Injected Mice. J Mol Neurosci 2016 Sep [PMID: 27112440]
Wezel A, de Vries MR, Maassen JM et al. Deficiency of the TLR4 analogue RP105 aggravates vein graft disease by inducing a pro-inflammatory response. Sci Rep. 2016 Apr 07 [PMID: 27053419] (IHC-P, Mouse) IHC-P Mouse
Kim HJ, Yoo HY, Jang JH et al. Wall stretch and thromboxane A2 activate NO synthase (eNOS) in pulmonary arterial smooth muscle cells via H2O2 and Akt-dependent phosphorylation. Pflugers Arch. 2016 Jan 04 [PMID: 26729266] (IHC-P, Rat) IHC-P Rat
Kober C, Rohn S, Weibel S et al. Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus traps. J Transl Med 2015 [PMID: 26149494] (WB) WB
Show All 22 Publications.

Reviews for iNOS Antibody (NB300-605) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 application: IF, WB.

Reviews using NB300-605:
Filter by Applications
IF
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence iNOS NB300-605
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reviewed by:
Anonymous
IF Mouse 11/13/2015
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Summary

ApplicationImmunofluorescence
Sample TestedMouse aortic root
SpeciesMouse

Blocking

Blocking Details30 minutes in normal donkey serum/TBST room temperature

Details

Detection NotesDetected Rhodamine Red from secondary antibody using fluorescence microscope
Fixation DetailsParaffin
Western Blot iNOS NB300-605
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reviewed by:
Leonid Tarassishin
WB Human 04/28/2014
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Summary

ApplicationWestern Blot
Sample Testedhuman astrocyte lysate
SpeciesHuman
LotNJ17385 1
Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)

Blocking

Blocking Details5% non-fat milk in PBS

Primary Anitbody

Dilution Ratio1:250, 18 hr, 4C, PBST (PBS+0.05%Tween 20)

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#Pierce/ThermoScientific, Cat. # 32460
Secondary Concentration1:500 (stock 10 ug/ml)

Details

Detection NotesECL-Pico (Piere/ThermoScientific), 1 min; Untreated and treated with IL-1beta cells; Loading Ctr: beta-Actin.

Comments

Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for iNOS Antibody (NB300-605). (Showing 1 - 1 of 1 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional iNOS Antibody Products

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Bioinformatics Tool for iNOS Antibody (NB300-605)

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Research Areas for iNOS Antibody (NB300-605)

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Blogs on iNOS.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
11/13/2015
Application: IF
Species: Mouse

Leonid Tarassishin
04/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot

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