iNOS Antibody


Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours more
Biological Strategies: Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) more
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, In vitro
0.5 mg/ml
Validated by:

Biological Strategies


iNOS Antibody Summary

Synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.
This antibody detects iNOS. It does not detect other NOS isoforms.
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:200 - 1:800
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:20
  • In vitro assay
Application Notes
WB: Detects an approx. 135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. Also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples. Use In vitro assay reported in scientific literature (PMID: 27998907).
iNOS Overexpression Lysate
iNOS Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
NB300-605 in the following applications:

Read Publications using
NB300-605 in the following applications:

  • 5 publications
  • IHC
    9 publications
  • 4 publications
  • WB
    22 publications

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
0.5 mg/ml
Affinity purified

Alternate Names for iNOS Antibody

  • EC
  • Hepatocyte NOS
  • HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS
  • NOS, type II
  • NOS2
  • NOS2A
  • NOS2ANOS type II


Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for iNOS Antibody (NB300-605)(41)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using NB300-605 Applications Species
Marek-Iannucci S, Thomas A, Hou J et al. Myocardial hypothermia increases autophagic flux, mitochondrial mass and myocardial function after ischemia-reperfusion injury Sci Rep Jul 10 2019 [PMID: 31292486] (IHC-P, Porcine) IHC-P Porcine
Singh, K;Gobert, AP;Coburn, LA;Barry, DP;Allaman, M;Asim, M;Luis, PB;Schneider, C;Milne, GL;Boone, HH;Shilts, MH;Washington, MK;Das, SR;Piazuelo, MB;Wilson, KT; Dietary Arginine Regulates Severity of Experimental Colitis and Affects the Colonic Microbiome Front Cell Infect Microbiol Mar 26 2019 [PMID: 30972302] (ICC/IF, Mouse) ICC/IF Mouse
Wang CN, Lin YC, Chang BC et al. Targeting the phosphorylation site of MARCKS alleviates symptoms of steroid-resistant like asthma in a murine model Br. J. Pharmacol. Feb 1 2019 [PMID: 30706455] (WB, Mouse) WB Mouse
Zhang H, Wei Q, Xiang X et al. Semaphorin 4A acts in a feed-forward loop with NF-kB pathway to exacerbate catabolic effect of IL-1b on chondrocytes. Int. Immunopharmacol. Jan 24 2019 [PMID: 30685700] (WB, Mouse) WB Mouse
Yu Y, Park SJ, Beyak MJ. Inducible nitric oxide synthase-derived nitric oxide reduces vagal satiety signalling in obese mice J. Physiol. (Lond.) Dec 18 2018 [PMID: 30565225] (WB, Mouse) WB Mouse
Zhang X, Zhao Y, Zhu X et al. Active vitamin D regulates macrophage M1/M2 phenotypes via the STAT-1-TREM-1 pathway in diabetic nephropathy J. Cell. Physiol. Nov 27 2018 [PMID: 30478987] (ICC/IF, Human) ICC/IF Human
Liu W, Li J, Cheng M et al. Zinc-Modified Sulfonated Polyetheretherketone Surface with Immunomodulatory Function for Guiding Cell Fate and Bone Regeneration Adv. Sci. Aug 7 2018 [PMID: 30356934] (IHC, Mouse) IHC Mouse
Zhu J, He J, Verano M et al. An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation. Lab Chip. Oct 10 2018 [PMID: 30302487] (ICC/IF, Mouse) ICC/IF Mouse
Lee S, Kim GL, Kim NY et al. ATF3 Stimulates IL-17A by Regulating Intracellular Ca2+/ROS-Dependent IL-1B Activation During Streptococcus pneumoniae Infection. Front Immunol Aug 30 2018 [PMID: 30214444] (WB, Mouse) WB Mouse
Choi JY, Yeo IJ, Kim KC et al. K284-6111 prevents the amyloid beta-induced neuroinflammation and impairment of recognition memory through inhibition of NF-kB-mediated CHI3L1 expression J Neuroinflammation Aug 11 2018 [PMID: 30098604] (WB, IHC, Mouse) WB, IHC Mouse
Show All 41 Publications.

Reviews for iNOS Antibody (NB300-605) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-605:
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Images Ratings Applications Species Date Details
Immunofluorescence iNOS NB300-605
reviewed by:
IF Mouse 11/13/2015


Sample TestedMouse aortic root


Blocking Details30 minutes in normal donkey serum/TBST room temperature


Detection NotesDetected Rhodamine Red from secondary antibody using fluorescence microscope
Fixation DetailsParaffin
Western Blot iNOS NB300-605
reviewed by:
Leonid Tarassishin
WB Human 04/28/2014


ApplicationWestern Blot
Sample Testedhuman astrocyte lysate
LotNJ17385 1
Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)


Blocking Details5% non-fat milk in PBS

Primary Anitbody

Dilution Ratio1:250, 18 hr, 4C, PBST (PBS+0.05%Tween 20)

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#Pierce/ThermoScientific, Cat. # 32460
Secondary Concentration1:500 (stock 10 ug/ml)


Detection NotesECL-Pico (Piere/ThermoScientific), 1 min; Untreated and treated with IL-1beta cells; Loading Ctr: beta-Actin.


Commentsbeta-Actin was detected with mouse anti-Actin antibody from Cell Signaling (1:500) and anti-mouse IgG, HRP from Pierce/ThermoScientific (1:500)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for iNOS Antibody (NB300-605). (Showing 1 - 1 of 1 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 NB300-605AF405
Alexa Fluor 488 NB300-605AF488
Alexa Fluor 647 NB300-605AF647
Alexa Fluor 700 NB300-605AF700
Biotin NB300-605B
DyLight 350 NB300-605UV
DyLight 405 NB300-605V
DyLight 488 NB300-605G
DyLight 550 NB300-605R
DyLight 594 NB300-605DL594
DyLight 650 NB300-605C
DyLight 680 NB300-605FR
DyLight 755 NB300-605IR
FITC NB300-605F
HRP NB300-605H
Janelia Fluor 549 NB300-605JF549
Janelia Fluor 646 NB300-605JF646

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Bioinformatics Tool for iNOS Antibody (NB300-605)

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews


Application: IF
Species: Mouse

Leonid Tarassishin
Application: WB
Species: Human


Gene Symbol NOS2