TOR/mTOR [p Ser2448] Antibody

Images

 
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours ...read more
Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, EM, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TOR/mTOR [p Ser2448] Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2440-2457 of human mTOR.
Modification
p Ser2448
Localization
Cell Membrane and Cytoplasmic
Specificity
Reactivity occurs with phosphorylated mTOR from human derived tissues and cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTOR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Electron Microscopy 1:10000 - 1:100000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Western blotting shows reactivity specific for phospho mTOR detecting a band at approximately 250 kDa.
Publications
Read Publications using
NB600-607 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Reactivity against mTOR from other species has not been determined, however, reactivity with Mouse is suggested based on protein sequence homologies.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TOR/mTOR [p Ser2448] Antibody

  • EC 2.7.11.1
  • FK506 binding protein 12-rapamycin associated protein 1
  • FK506 binding protein 12-rapamycin associated protein 2
  • FK506-binding protein 12-rapamycin complex-associated protein 1
  • FKBP12-rapamycin complex-associated protein
  • FLJ44809
  • FRAP
  • FRAP1
  • FRAP1FKBP12-rapamycin complex-associated protein 1
  • FRAP2
  • Mammalian target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • Mechanistic target of rapamycin
  • MTOR
  • RAFT1
  • rapamycin and FKBP12 target 1
  • rapamycin associated protein FRAP2
  • Rapamycin target protein 1
  • RAPT1FKBP-rapamycin associated protein
  • serine/threonine-protein kinase mTOR
  • TOR

Background

Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the S. cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TOR/mTOR Antibody (NB600-607)(5)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Rat
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using NB600-607 Applications Species
Zhou X, Yue GG, Chan AM et al. Eriocalyxin B, a novel autophagy inducer, exerts anti-tumor activity through the suppression of Akt/mTOR/p70S6K signaling pathway in breast cancer Biochem. Pharmacol. 2017 Jun 30 [PMID: 28669564] (IHC-P, Human) IHC-P Human
Lisi L, Laudati E, Navarra P, Dello Russo C. The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype. J Neuroinflammation 2014 Jul 23 [PMID: 25051975] (WB, Rat)

Details:
mTOR [p Ser2448] antibody used for WB on whole-cell lysates from rat's microglial cells activated by control-conditioned medium / C-CM or CM from C6 glioma cells activated with lipopolysaccharide/LPS or IFN-gamma (Figure 1).
WB Rat
Lisi L, Stigliano E, Lauriola L et al. Proinflammatory-activated glioma cells induce a switch in microglial polarization and activation status, from a predominant M2b phenotype to a mixture of M1 and M2a/b polarized cells. ASN Neuro 2014 Apr 01 [PMID: 24689533] (WB, Rat) WB Rat
Lisi L, Navarra P, Feinstein DL, Dello Russo C. The mTOR kinase inhibitor rapamycin decreases iNOS mRNA stability in astrocytes. J Neuroinflammation 2011 Jan 5 [PMID: 21208419] (WB, Rat) WB Rat
Gupta M, Dillon SR, Ziesmer SC et al. A proliferation-inducing lig mediates follicular lymphoma B-cell proliferation cyclin D1 expression through phosphatidylinositol 3-kinase-regulated mammalian target of rapamycin activation. Blood;113(21):5206-5216. 2009 [PMID: 19321861]

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Secondary Antibodies

 

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Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

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Bioinformatics

Gene Symbol MTOR
Entrez
Uniprot