TOR/mTOR [p Ser2448] Antibody

Images

 
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a ...read more
Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - affinity purified TOR/TOR/mTOR [p Ser2448] antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and ...read more
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at ...read more
Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a ...read more
N/A: TOR/mTOR [p Ser2448] Antibody [NB600-607]
ELISA: TOR/mTOR [p Ser2448] Antibody [NB600-607] - ELISA Results of TOR/TOR/mTOR [p Ser2448] antibody tested against BSA-conjugated non-phospho [purple line] and phospho [blue line] forms of immunizing peptide. Each ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, NULL
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TOR/mTOR [p Ser2448] Antibody Summary

Immunogen
TOR/TOR/mTOR [p Ser2448] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding a c-terminal region near Serine 2448 of human TOR/mTOR. (Uniprot: P42345)
Modification
p Ser2448
Localization
Cell Membrane and Cytoplasmic
Specificity
Reactivity occurs with phosphorylated TOR/mTOR from human derived tissues and cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTOR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000-1:100000
  • Immunohistochemistry 5.0 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • N/A
  • Western Blot 1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in immunohistochemistry, ELISA and western blotting. Western blotting shows reactivity specific for phospho mTOR detecting a band at approximately 250 kDa. Reactivity in other immunoassays is unknown.
Theoretical MW
288.89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NB600-607 in the following applications:

Publications
Read Publications using
NB600-607 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    7 publications

Reactivity Notes

Reactivity against TOR/mTOR from other species has not been determined, however, reactivity with mouse is suggested based on protein sequence homologies

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for TOR/mTOR [p Ser2448] Antibody

  • EC 2.7.11.1
  • FK506 binding protein 12-rapamycin associated protein 1
  • FK506 binding protein 12-rapamycin associated protein 2
  • FK506-binding protein 12-rapamycin complex-associated protein 1
  • FKBP12-rapamycin complex-associated protein
  • FLJ44809
  • FRAP
  • FRAP1
  • FRAP1FKBP12-rapamycin complex-associated protein 1
  • FRAP2
  • Mammalian target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • Mechanistic target of rapamycin
  • MTOR
  • RAFT1
  • rapamycin and FKBP12 target 1
  • rapamycin associated protein FRAP2
  • Rapamycin target protein 1
  • RAPT1FKBP-rapamycin associated protein
  • serine/threonine-protein kinase mTOR
  • TOR

Background

Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the S. cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB100-1595
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP1-33116
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-46404
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
NB200-157
Species: Hu
Applications: IHC, IHC-P, KO, WB
NBP1-87799
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB600-1049
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
AF847
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, KO, Simple Western, WB
DVE00
Species: Hu
Applications: ELISA
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
MAB40401
Species: Hu, Mu
Applications: ICC, IHC, WB
NBP2-67627
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-74653
Species: Hu, Mu, Rt
Applications: IHC, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB100-81898
Species: Hu, Po
Applications: ICC/IF, IHC, IHC-P, WB
NB600-607
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P, NULL

Publications for TOR/mTOR Antibody (NB600-607)(9)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(1)
WB
(7)
All Applications
Filter By Species
Human
(5)
Rat
(3)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB600-607 Applications Species
Lisi L, Ciotti GMP, Chiavari M et al. Phospho-mTOR expression in human glioblastoma microglia-macrophage cells Neurochem. Int. 2019 Jun 10 [PMID: 31195027] (WB, IHC, Human) WB, IHC Human
Mahmoud S, Gharagozloo M, Simard C et al. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line Brain Sci May 15 2019 [PMID: 31096716] (WB, Human) WB Human
Zhou X, Yue GG, Chan AM et al. Eriocalyxin B, a novel autophagy inducer, exerts anti-tumor activity through the suppression of Akt/mTOR/p70S6K signaling pathway in breast cancer Biochem. Pharmacol. 2017 Jun 30 [PMID: 28669564] (IHC-P, Human) IHC-P Human
Lisi L, Laudati E, Navarra P, Dello Russo C. The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype. J Neuroinflammation 2014 Jul 23 [PMID: 25051975] (WB, Rat)

Details:
mTOR [p Ser2448] antibody used for WB on whole-cell lysates from rat's microglial cells activated by control-conditioned medium / C-CM or CM from C6 glioma cells activated with lipopolysaccharide/LPS or IFN-gamma (Figure 1).
WB Rat
Lisi L, Stigliano E, Lauriola L et al. Proinflammatory-activated glioma cells induce a switch in microglial polarization and activation status, from a predominant M2b phenotype to a mixture of M1 and M2a/b polarized cells. ASN Neuro 2014 Apr 01 [PMID: 24689533] (WB, Rat) WB Rat
Lisi L, Navarra P, Feinstein DL, Dello Russo C. The mTOR kinase inhibitor rapamycin decreases iNOS mRNA stability in astrocytes. J Neuroinflammation 2011 Jan 5 [PMID: 21208419] (WB, Rat) WB Rat
Cappoli N, Mezzogori D, Tabolacci E, Coletta I THE mTOR KINASE INHIBITOR RAPAMYCIN ENHANCES THE EXPRESSION AND RELEASE OF PRO-INFLAMMATORY CYTOKINE INTERLEUKIN 6 MODULATING THE ACTIVATION OF HUMAN MICROGLIAL CELLS EXCLI Journal Sep 6 2019 (WB, Human) WB Human
Serafin V, PorcU E, Cortese G et al. SYK Targeting Represents a Potential Therapeutic Option for Relapsed Resistant Pediatric ETV6-RUNX1 B-Acute Lymphoblastic Leukemia Patients Int J Mol Sci Dec 7 2019 [PMID: 31817853] (WB, Human) WB Human
Gupta M, Dillon SR, Ziesmer SC et al. A proliferation-inducing lig mediates follicular lymphoma B-cell proliferation cyclin D1 expression through phosphatidylinositol 3-kinase-regulated mammalian target of rapamycin activation. Blood;113(21):5206-5216. 2009 [PMID: 19321861]

Reviews for TOR/mTOR Antibody (NB600-607) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB600-607:
Filter by Applications
WB
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot TOR/mTOR NB600-607
Enlarge
4
reviewed by:
Lucia Lisi
WB Human 01/17/2020
View

Summary

ApplicationWestern Blot
Sample TestedHuman microglia cell lysate
SpeciesHuman
Lot29098
Immunohistochemistry-Paraffin TOR/mTOR NB600-607
Enlarge
4
reviewed by:
Lucia Lisi
IHC-P Human 01/17/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Brain Tissue
SpeciesHuman
Lot29098

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TOR/mTOR Antibody (NB600-607) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TOR/mTOR Products

Bioinformatics Tool for TOR/mTOR Antibody (NB600-607)

Discover related pathways, diseases and genes to TOR/mTOR Antibody (NB600-607). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TOR/mTOR Antibody (NB600-607)

Discover more about diseases related to TOR/mTOR Antibody (NB600-607).
 

Pathways for TOR/mTOR Antibody (NB600-607)

View related products by pathway.

PTMs for TOR/mTOR Antibody (NB600-607)

Learn more about PTMs related to TOR/mTOR Antibody (NB600-607).
 

Research Areas for TOR/mTOR Antibody (NB600-607)

Find related products by research area.

Blogs on TOR/mTOR.

Staufen1 Overabundance and the Consequent mTOR Hyperactivity in Amyotrophic Lateral Sclerosis, Spinocerebellar Ataxia Type 2, Alzheimer’s, Parkinson’s, and Huntington’s Diseases
Jamshed Arslan, Pharm D, PhD Neurodegenerative disorders involve loss of function and, ultimately, death of neurons. Selective neuronal vulnerability has been observed in a variety of neurodegenerative diseases. Fo...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

The Many Connections Between Autophagy and Kidney Disease
By Yoskaly Lazo-Fernandez, PhD The first description of what is called today an autophagosome was given in a paper published in 1957. Its author employed electron microscopy to observe the neonatal features of mous...  Read full blog post.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Lucia Lisi
01/17/2020
Application: WB
Species: Human

Lucia Lisi
01/17/2020
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol MTOR
Entrez
Uniprot