NIH 3T3 CoCl2 treated/Untreated Cell Lysate


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1.0 mg/ml

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NIH 3T3 CoCl2 treated/Untreated Cell Lysate Summary

NIH 3T3 cells are grown under standard tissue culture conditions until semi-confluent (70-80%). The cells are then treated with or without 100 uM CoCl2 for 4 hours, washed in PBS and directly lysed into 1X Laemmli sample buffer with BME. The lysate is sonicated and tested by Western blot.


  • SDS-Page
Application Notes
NIH 3T3 whole cell lysate is provided as a Western blot positive control. The lysates are provided as a set of treated and untreated samples. Use 10 ul per lane for a standard mini-gel blot (1.0 mg/ml).

Packaging, Storage & Formulations

Store at -20 to -80C. Avoid freeze-thaw cycles.
The protein lysate is prepared in 1x Laemmli sample buffer with BME. Boiling before loading is not necessary.
1.0 mg/ml

Lysate Details for Array

Subcellular Fraction
CoCl2 treated/Untreated


NIH3T3 cells are mouse embryonic fibroblast cells that are derived from Swiss mouse embryonic tissue. 3T3 is an abbreviation of 3-day transfer, inoculum 3 x 10^5 cells. NIH3T3 cells are used primarily in the cultivation of keratinocytes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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