The Role of LC3 within the Autophagic Pathway

Tue, 07/27/2010 - 08:14


We at Novus Biologicals have a broad antibody databasecovering the area of autophagy - over 1400 reagents in total. Autophagy is bulk degradation of cytoplasmic components - literally, self-digestion of the cell. Double-membrane vesicles, called autophagosomes, carry unwanted cell components to the lysosomes within an inner autophagic membrane. They then fuse liberating the autophagic body and its contents into the lumen of the vacuole for degradation.

This is a complex process involving at least 16 proteins. However LC3 is the only one known to form a stable association with the membrane of autophagosomes. It is known to exist in two forms: LC3-I, which is found in the cytoplasm and LC3-II, which is membrane-bound and is converted from LC3-I, to initiate formation and lengthening of the autophagosome. It differs from LC3-1 only in the fact it is covalently modified with lipid extensions (lipidation) and has undergone removal of a short amino acid. Detection of this conversion, using LC3 antibodies, is a useful biomarker to detect autophagy – in fact, it’s the only reliable one.

Immunohistochemistry: LC3 Antibody Immunohistochemistry: LC3 Antibody

LC3 is known to use post translational modifications (PTMs) during the autophagic response. PTM, the chemical modification of proteins following translation, is a late stage in protein biosynthesis and can radically affect protein function. The mechanism by which LC3 utilizes post translational modification is crucial to understanding how the autophagic process works, and how it influences certain diseases such as cancer.

The featured autophagy products in our antibody catalog include wide selections of quality guaranteed LC3LC3B, Bcl2, ATG5 and HIF-1 alpha reagents.

Novus Biologicals offers many LC3 reagents for your research needs including:

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