ProteinSimple - Simple Western Certified

Antibodies for Simple Western

Simple Westerns separate proteins by size, and precisely manages immunoprobing, incubations, washes and even detection steps by removing the manual, error-prone steps in standard western blots. Over 1,300 Novus Biologicals and R&D Systems antibodies have been certified on ProteinSimple's Simple Western platforms. View our complete portfolio of Simple Western validated antibodies. If your antibody hasn’t been validated for Simple Western, you can submit your results to our Innovator's Reward Program for a voucher for the full purchase price for Risk-Free Testing.

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Simple Western FAQs

If your antibody of choice has not yet been screened in Simple Western, Novus Biologicals will reward you for being the first to test the product in this application. Learn more about Novus Rewards and their benefits.


Example data from traditional Western blot vs. Simple Western

Traditional Western Blots

Simple Western

HEK293 human embryonickidney cell line mock transfected or transfectedwith full length human cIAP-1 (Cat No MAB818)

Detection of Human cIAP 1/HIAP 2 by Western Blot. HEK293 human embryonic kidney cell line mock transfected or transfected with full length human cIAP-1. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB818) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for cIAP‑1/HIAP‑2 at approximately 72 kDa.

Simple Western lane view using lysates of HeLa human cervicalepithelial carcinoma parental cell line and cIAP‑1/HIAP‑2 knockout HeLa cell line (KO) to determine postive cIAP1 expression in HeLa Wildtype cells at 66kDa using MAB818.

Detection of Human cIAP 1/HIAP 2 by Simple Western. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP‑1/HIAP‑2 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) in the parental HeLa cell line but is not detectable in knockout HeLa cell line. Mouse Anti-Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB818) was used at 5 µg/mL. This experiment was conducted under reducing conditions with the 12-230 kDa separation system.

Positive expression of HSP60 in Western Blot using Catalog Number AF1800.

Detection of Human/Mouse/Rat HSP60 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF 7 human breast cancer cell line, NIH 3T3 mouse embryonic fibroblast cell line, and Nb2 11 rat lymphoma cell line. A specific band was detected for HSP60 (Catalog # AF1800, R&D Systems) at approximately 62 kDa (as indicated). See full datasheet.  

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and NIH 3T3 cells detect HSP69 using AF1800.

Detection of Human and Mouse HSP60 by Simple Western. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and NIH 3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP60 (Catalog # AF1800, R&D Systems) at approximately 60 kDa (as indicated).This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. See full datasheet.  

View ProteinSimple Instruments to compare experimental time, sample number, detection method as well as size or charge separation capabilities to best fit your research needs.