HeLa CoCl2 treated/Untreated Cell Lysate


Western Blot: HeLa Hypoxic Simulated / Normoxic Tissue Lysate [NBP2-36450] - Western blot analysis of HIF-1 alpha antibody (NB100-134) in HeLa cobalt chloride treated and normoxic lysates (NBP2-36450). Tubulin loading ...read more

Product Details

Reactivity HuSpecies Glossary
Applications PAGE
1.0 mg/ml

Order Details

HeLa CoCl2 treated/Untreated Cell Lysate Summary

HeLa cells are cultured using standard media condition until semi-confluent (70-80%). The cells are then treated with or without 100 uM CoCl2 for 4 hours. The cells are then washed in PBS and lysed into 1X Laemmli sample buffer with BME. The lysate is sonicated and tested by Western blot for reactivity to Hif-1a using a polyclonal anti-Hif-1a antibody (NB100-134). Tubulin reactivity of each lysate is shown as a loading control.


  • SDS-Page
Application Notes
HeLa Whole cell lysate is provided as a Western blot positive control for Hif-1 research. The lysates are provided as a set of treated and untreated samples. Use 10 ul per lane for a standard mini-gel blot (approx. 1 mg/ml).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
The protein lysate is prepared in 1x Laemmli sample buffer with BME. Boiling before loading is not necessary.
1.0 mg/ml

Lysate Details for Array

Subcellular Fraction
CoCl2 treated/Untreated

Kit Components

  1. 0.1 ml of HeLa Cobalt Chloride treated whole lysate
  2. 0.1 ml of HeLa normoxic whole lysate


Cobalt Chloride (CoCl2) treated cells are often used as a positive control for HIF-1 and HIF-2 alpha analysis in hypoxia signaling experiments. HIFs induction can be conveniently achieved in vitro through CoCl2 treatment of cultured cells. CoCl2 inhibits prolyl-hydroxylases (PHD enzymes), the oxygen sensors, through replacement of Fe with Co making these enzymes unable to mark HIF alpha for degradation. In other words, CoCl2 allosterically blocks PHDs through its metal ion binding domain, hence, abolishing the VHL-HIF interaction which ultimately leads to the accumulation of HIFs in CoCl2 treated cells. CoCl2 has been regarded as one of the most reliable HIF-1 alpha inducer or hypoxia response mimicker, and this chemically induced hypoxia model is widely used in hypoxia-signaling research. Many researcher use Novus antibodies to Hif-1 and Hif-2 in Western blot analysis as markers for Hypoxia. For this reason it is important to have reliable positive controls for your Western blot to ensure that your antibodies and test conditions are optimized. Novus Biologicals is pleased to offer the same positive control cell lysates used to validate antibodies to Hif-1 and Hif-2 to our customers for their research. For Hif-1, we have matched pairs of lysate from HeLa cells treated with and without CoCl2.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for HeLa Lysate (NBP2-36450) (0)

There are no publications for HeLa Lysate (NBP2-36450).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HeLa Lysate (NBP2-36450) (0)

There are no reviews for HeLa Lysate (NBP2-36450). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for HeLa Lysate (NBP2-36450) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HeLa Products

Array NBP2-36450

Research Areas for HeLa Lysate (NBP2-36450)

Find related products by research area.

Blogs on HeLa.

The Role of LC3 within the Autophagic Pathway
We at Novus Biologicals have a broad antibody database covering the area of autophagy - over 1400 reagents in total. Autophagy is the bulk degradation of cytoplasmic components - literally, self-digestion of the cell. Double-membrane vesicles, called ...  Read full blog post.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our HeLa CoCl2 treated/Untreated Cell Lysate and receive a gift card or discount.