HIF-1 alpha Antibody (H1alpha67)

Images

 
Biological Strategies: Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. WB image submitted by a verified customer review.
Genetic Strategies: Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells ...read more
Biological Strategies: Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under ...read more
Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF-1 alpha in human kidney using NB100-105. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and ...read more
Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5 x 10^6) were stained with ...read more
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF1alpha/PFKFB3 is upregulated in beta-cells of HIP rats and humans with type 2 diabetes. Representative Western blot of PFKFB3 and HIF1alpha levels in ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line ...read more
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the biotin conjugate of NB100-105. Staining of human glioblastoma multiforme.
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Upregulation of HIF-1alpha in human PCa. Protein expression of HIF-1alpha, VEGF, and GLUT4 were examined with western blot, in PC-3, DU145, and LNCaP cells ...read more
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using HIF-1 alpha Antibody on human throat cancer tissue. PFA fixed paraffin-embedded sections. Primary antibody dilution 1:100. ...read more
Biological Strategies: Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis of HIF-1 alpha in human hepatocytes from cancer patient using anti-HIF-1 alpha antibody NB100-105. Western blot image ...read more
Biological Strategies: Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50 ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) ...read more
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - L. donovani infection induces HIF-1 alpha expression in CD11chi splenic DCs in an IRF-5 dependent manner. Mice were infected with 2 x 10^7 amastigotes ...read more
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Upregulation of HIF-1alpha in human Pca. HIF-1alpha protein was detected by western blot in nonmalignant (RWPE-1 and BPH1) and PCa cell lines (PC-3, DU145, ...read more
Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1alpha expression in PDAC and adjacent normal pancreatic tissue. PDAC with weak (intensity 1) nuclear and cytoplasmic HIF-1alpha staining (left). ...read more
Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor(R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in H929 cells using HIF-1 alpha antibody. Flow cytometry image ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Fe, Ma, Pm, Pm, Rb, Sh, XpSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, LA, PLA, TCS, IHC-FrFl, KD, KO
Clone
H1alpha67
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HIF-1 alpha Antibody (H1alpha67) Summary

Immunogen
This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
Localization
HIF-1 is a nuclear protein
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HIF1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1 - 5 ug/IP
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Gel Super Shift Assays 1:1-1:100
  • Immunoassay
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:20-1:50
  • Immunohistochemistry-Frozen 1:20-1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • Knockdown Validated
  • Knockout Validated
  • Ligand Activation
  • Proximity Ligation Assay
  • Simple Western
  • Tissue Culture Substratum
  • Western Blot 1:500
Application Notes
Use in IHC-FrFl reported in scientific literature (PMID: 33242463). Use in Knockdown Validated reported in scientific literature (PMID: 32772041). ChIP usage was reported in scientific literature. ELISA usage was reported in scientific literature (PMID: 20042684). Gel Super Shift Assays usage was reported in scientific literature (PMID: 22411794). Ligand Activation usage was reported in scientific literature (PMID: 26147748). Use in Immunoassay reported in scientific literature (PMID: 26147748). Use in vitro assay reported in multiple pieces of scientific literature. In WB, a band can be seen at 120 Knockdown Validateda representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. Knock Out Validation was reported in scientific literature (PMID: 26861754). Use in proximity ligation assay reported in scientific literature (PMID: 27595394). Use in Immunoblotting reported in multiple pieces of scientific literature. We recommend the use of a highly sensitive ECL reagent, such as West Pico PLUS, for Western blot detection. Simple Western reported by an internal validation. Separated by Size
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
COS-7 Nuclear Hypoxic Induced Cell Lysate
HeLa CoCl2 treated/Untreated Cell Lysate
HepG2 CoCl2 treated/Untreated Cell Lysate
HeLa Hypoxic / Normoxic Cell Lysate
HepG2 Hypoxic / Normoxic Cell Lysate
HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
NIH 3T3 CoCl2 treated/Untreated Cell Lysate
L929 CoCl2 treated/Untreated Cell Lysate
Raw 264.7 CoCl2 treated/Untreated Cell Lysate
PC12 CoCl2 treated/Untreated Cell Lysate
PC12 Hypoxic / Normoxic Cell Lysate
Raw 264.7 Hypoxic / Normoxic Cell Lysate
L929 Hypoxic / Normoxic Cell Lysate
NIH 3T3 Hypoxic / Normoxic Cell Lysate
Reviewed Applications
Read 45 Reviews rated 4.2
using
NB100-105 in the following applications:

Publications
Read Publications using
NB100-105 in the following applications:

  • 54 publications
  • 6 publications
  • 3 publications
  • 1 publication
  • GS
    7 publications
  • IA
    1 publication
  • IB
    1 publication
  • 65 publications
  • IF
    1 publication
  • IHC
    141 publications
  • 6 publications
  • 1 publication
  • 100 publications
  • 1 publication
  • IP
    32 publications
  • KD
    1 publication
  • PLA
    2 publications
  • WB
    485 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33816617).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HIF-1 alpha Antibody (H1alpha67)

  • AINT
  • anti-HIF-1 alpha
  • anti-HIF1A
  • ARNT interacting protein
  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • BHLHE78
  • Class E basic helix-loop-helix protein 78
  • H1alpha67
  • HIF 1A
  • HIF1 alpha
  • HIF-1 alpha
  • HIF1
  • HIF1A
  • HIF-1a
  • HIF-1alpha
  • HIF-1-alpha
  • HIF1-alpha
  • hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • hypoxia-inducible factor 1-alpha
  • Member of PAS protein 1
  • member of PAS superfamily 1
  • MOP1
  • PAS domain-containing protein 8
  • PASD8

Background

Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease (1). In cancer and particularly solid tumors, hypoxia plays a critical role in the regulation of genes involved in stem cell renewal, epithelial to mesenchymal transition (EMT), metastasis and angiogenesis. In the tumor microenvironment (TME), hypoxia influences the properties and function of stromal cells (e.g., fibroblasts, endothelial and immune cells) and is a strong determinant of tumor progression (2,3).

HIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. In its active form under hypoxic conditions, HIF-1 is stabilized by the formation of a heterodimer of HIF-1 alpha and ARNT/HIF-1 beta subunits. Nuclear HIF-1 engages p300/CBP for binding to hypoxic response elements (HREs). This process induces transcription and regulation of genes including EPO, VEGF, iNOS2, ANGPT1 and OCT4 (4,5).

Under normoxic conditions, the HIF-1 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system. This process is mediated by prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-402 and Pro-564) within the oxygen-dependent degradation domain of HIF-1 alpha. Once hydroxylated, HIF-1 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (4). pVHL dependent regulation of HIF-1 alpha plays a role in normal physiology and disease states. Regulation of HIF-1 alpha by pVHL is critical for the suppressive function of FoxP3+ regulatory Tcells (6). Repression of pVHL expression in chronic lymphocytic leukemia (CLL) B cells leads to HIF-1 alpha stabilization and increased VEGF secretion (7).

References

1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.

2. Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413

3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y

4. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483

5. Dengler, V. L., Galbraith, M. D., & Espinosa, J. M. (2014). Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology. https://doi.org/10.3109/10409238.2013.838205

6. Lee, J. H., Elly, C., Park, Y., & Liu, Y. C. (2015). E3Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1 alpha to Maintain Regulatory T Cell Stability and Suppressive Capacity. Immunity. https://doi.org/10.1016/j.immuni.2015.05.016

7. Ghosh, A. K., Shanafelt, T. D., Cimmino, A., Taccioli, C., Volinia, S., Liu, C. G., ... Kay, N. E. (2009). Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood. https://doi.org/10.1182/blood-2008-10-185686

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIF-1 alpha Antibody (NB100-105)(939)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Bovine, Canine, Porcine, Primate, Rabbit, Sheep, Xenopus.

We have publications tested in 18 applications: ChIP, ELISA, EMSA, FLOW, GS, IA, IB, ICC/IF, IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, IHC/IF, IP, KD, PLA, WB.


Filter By Application
ChIP
(54)
ELISA
(6)
EMSA
(3)
FLOW
(1)
GS
(7)
IA
(1)
IB
(1)
ICC/IF
(65)
IF
(1)
IHC
(141)
IHC-Fr
(6)
IHC-FrFl
(1)
IHC-P
(100)
IHC/IF
(1)
IP
(32)
KD
(1)
PLA
(2)
WB
(485)
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Human
(420)
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(250)
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(124)
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(5)
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(2)
Rabbit
(12)
Sheep
(3)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 939. Show All 939 Publications.
Publications using NB100-105 Applications Species
Qin Y, Dinabandhu A, Cao X et al. ANGPTL4 influences the therapeutic response of neovascular age-related macular degeneration patients by promoting choroidal neovascularization JCI insight Jun 2 2022 [PMID: 35653189] (IHC, Mouse) IHC Mouse
He WZ, Yang M, Jiang Y et al. miR-188-3p targets skeletal endothelium coupling of angiogenesis and osteogenesis during ageing Cell death & disease May 25 2022 [PMID: 35610206] (WB, Mouse) WB Mouse
Jacquier V, Gitenay D, Fritsch S et al. RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53 Cellular and molecular life sciences : CMLS May 3 2022 [PMID: 35501580] (WB, Mouse)

Details:
Fig. 1
WB Mouse
Kelly YM, Ward C, Zhang R et al. Effects of Multi-Stage Procurement on the Viability and Function of Human Donor Parathyroid Glands The Journal of surgical research Apr 22 2022 [PMID: 35468367] (IHC-P, Human) IHC-P Human
Inada A, Inada O, Yasunami Y et al. Amelioration of murine diabetic nephropathy with a SGLT2 inhibitor is associated with suppressing abnormal expression of hypoxia-inducible factors The American journal of pathology Apr 20 2022 [PMID: 35460614] (IHC, Mouse) IHC Mouse
Xia QS, Wu F, Wu WB et al. Berberine reduces hepatic ceramide levels to improve insulin resistance in HFD-fed mice by inhibiting HIF-2 alpha Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie Apr 13 2022 [PMID: 35429745] (IHC-P, WB, ICC/IF, Human, Mouse) IHC-P, WB, ICC/IF Human, Mouse
Geng B, Wang X, Park KH et al. UCHL1 protects against ischemic heart injury via activating HIF-1 alpha signal pathway Redox biology Mar 18 2022 [PMID: 35339825] (ICC/IF, Human) ICC/IF Human
Min, J;Ma, F;Seyran, B;Pellegrini, M;Greeff, O;Moncada, S;Tudzarova, S; beta-cell-specific deletion of PFKFB3 restores cell fitness competition and physiological replication under diabetogenic stress Communications biology [PMID: 35318430] (ICC/IF, Mouse) ICC/IF Mouse
Li M, Su Y, Gao X et al. Transition of autophagy and apoptosis in fibroblasts depends on dominant expression of HIF-1 alpha or p53 Journal of Zhejiang University. Science. B Mar 15 2022 [PMID: 35261216] (IHC-P, WB, Human) IHC-P, WB Human
Chen TH, Liu CT, Cheng Cy et al. Oligosaccharides Ameliorate Acute Kidney Injury by Alleviating Cluster of Differentiation 44-Mediated Immune Responses in Renal Tubular Cells Nutrients Feb 11 2022 [PMID: 35215410]
Show All 939 Publications.

Reviews for HIF-1 alpha Antibody (NB100-105) (45) 4.245

Average Rating: 4.2
(Based on 45 reviews)
We have 45 reviews tested in 6 species: Human, Mouse, Rat, Other, also tested in mouse and rat, 0.

Reviews using NB100-105:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
Western Blot
(38)
Affinity Purification
(1)
Immunohistochemistry
(1)
Immunofluorescence
(2)
Immunocytochemistry
(2)
All Applications
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Human
(23)
Mouse
(12)
Rat
(5)
Other
(3)
also tested in mouse and rat
(1)
0
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin HIF-1 alpha NB100-105
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5
reviewed by:
Sarah Miller
Immunohistochemistry-Paraffin Human 01/19/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedThroat cancer tissue
SpeciesHuman
LotH1alpha67

Comments

CommentsPFA fixed paraffin-embedded sections. Primary antibody dilution 1:100.
Western Blot HIF-1 alpha NB100-105
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5
reviewed by:
Toni johanson
Western Blot Human 03/31/2021
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Summary

ApplicationWestern Blot
Sample TestedCaki-1 cell lysate,fibroblasts
SpeciesHuman
LotBQ
Western Blot HIF-1 alpha NB100-105
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5
reviewed by:
Betty Poon
Western Blot Human 08/09/2020
View

Summary

ApplicationWestern Blot
Sample TestedHEK293A whole cell lysate,RCC4 Whole cell lysate
SpeciesHuman
LotBH-23
Western Blot HIF-1 alpha NB100-105
Enlarge
5
reviewed by:
Rajnish Singh
Western Blot Human 06/13/2019
View

Summary

ApplicationWestern Blot
Sample TestedBlood mononuclear cells (PBMCs)
SpeciesHuman
LotBH-13

Comments

CommentsConfirmation of induction of hypoxia in CoCl2 treated PBMCs infected with vGPCR frame shift knock out and wild type revertant KSHV virion. KSHV infected cells were treated with CoCl2 for 48 hours followed by protein isolation and western blot against HIF1 alpha .
  3
reviewed by:
Marie Lassont
Western Blot Human 02/02/2019
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman
LotAB6
  4
reviewed by:
Steve Rey
Western Blot Rat 02/02/2019
View

Summary

ApplicationWestern Blot
Sample TestedRat liver
SpeciesRat
LotAW1
Western Blot HIF-1 alpha NB100-105
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4
reviewed by:
Anthony Morales
Western Blot Human 01/29/2019
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Summary

ApplicationWestern Blot
Sample Testedhuman adrenal cancer cells (whole cell lysate)
SpeciesHuman
Lotunknown
Western Blot HIF-1 alpha NB100-105
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4
reviewed by:
Arthur Von Houtt
Western Blot Rat 01/29/2019
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Summary

ApplicationWestern Blot
Sample TestedRat liver
SpeciesRat
LotAW-1

Comments

CommentsDetection Method: ECL
Western Blot HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Arthur Von Houtt
Western Blot Human 01/24/2019
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
LotAB7

Comments

CommentsECL solution for 5min, then exposure to film 3-5min
  3
reviewed by:
David White
Western Blot Human 01/23/2019
View

Summary

ApplicationWestern Blot
Sample Tested293t cell lysate
SpeciesHuman
LotNB100-105
Western Blot HIF-1 alpha NB100-105
Enlarge
3
reviewed by:
Jun Hamazaki
Western Blot Human 06/29/2017
View

Summary

ApplicationWestern Blot
Sample Tested293t cell lysate
SpeciesHuman
Lotbh-1
Western Blot HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Lauren Zenewicz
Western Blot Mouse 02/01/2017
View

Summary

ApplicationWestern Blot
Sample TestedCD4 T cells cultured in vitro, stimulated with anti-CD3/anti-CD28
SpeciesMouse

Comments

CommentsFigure Legend: Naïve CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1a–deficient (Hif1a KO) mice were differentiated under IL-22–skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1a and lamin B1 levels were analyzed by Western blot.
  5
reviewed by:
Lisa Ikariyama
Affinity Purification also tested in mouse and rat 12/03/2016
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Summary

ApplicationAffinity Purification
Sample Tested2 Sample tested
Speciesalso tested in mouse and rat
LotN/A
Western Blot HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Verified Customer
Western Blot Mouse 08/17/2016
View

Summary

ApplicationWestern Blot
Sample TestedHL-1 cardiomyocytes
SpeciesMouse
  4
reviewed by:
MENG xing
Western Blot Human 05/08/2016
View

Summary

ApplicationWestern Blot
Sample Testedk562 cells
SpeciesHuman
LotBA-3
  4
reviewed by:
Betty Poon
Western Blot Human 04/18/2016
View

Summary

ApplicationWestern Blot
Sample TestedHEK293A whole cell lysate
SpeciesHuman
LotAW-1
Western Blot HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Verified Customer
Western Blot Mouse 03/24/2016
View

Summary

ApplicationWestern Blot
SpeciesMouse
LotBD-10
Western Blot HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Amruta Ashtekar
Western Blot Mouse 10/07/2015
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Summary

ApplicationWestern Blot
Sample TestedCell lysate
SpeciesMouse
Western Blot HIF-1 alpha NB100-105
Enlarge
5
reviewed by:
Verified Customer
Western Blot Mouse 10/05/2015
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Summary

ApplicationWestern Blot
Sample Testedmouse retina
SpeciesMouse
LotNB100-479
  3
reviewed by:
Haiquan Lu
Western Blot Human 08/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman
Western Blot HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Lakshmi Reddy Bollu
Western Blot Human 08/10/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman prostate cancer cell line protein samples
SpeciesHuman
Western Blot HIF-1 alpha NB100-105
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5
reviewed by:
Liyong Zhang
Western Blot Human 04/28/2015
View

Summary

ApplicationWestern Blot
SpeciesHuman
  3
reviewed by:
cynthia morimoto
Western Blot Mouse 12/17/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PIMD: 23148210
SpeciesMouse
Western Blot HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Verified Customer
Western Blot Human 07/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedCaki-1 cell lysate
SpeciesHuman
  5
reviewed by:
Aparna Akolkar
Western Blot Human 07/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedHela Cell Lysate
SpeciesHuman
Western Blot HIF-1 alpha NB100-105
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human 05/09/2014
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Western Blot HIF-1 alpha NB100-105
Enlarge
5
reviewed by:
Cidi Chen
Western Blot Mouse 04/28/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse brain lysate
SpeciesMouse
Western Blot HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Verified Customer
Western Blot Mouse 04/28/2014
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Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
Immunohistochemistry HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Verified Customer
Immunohistochemistry Other 04/14/2014
View

Summary

ApplicationImmunohistochemistry
SpeciesOther
Immunofluorescence HIF-1 alpha NB100-105
Enlarge
4
reviewed by:
Verified Customer
Immunofluorescence Other 04/14/2014
View

Summary

ApplicationImmunofluorescence
SpeciesOther
  4
reviewed by:
Verified Customer
Western Blot Mouse 03/28/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
Western Blot HIF-1 alpha NB100-105
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5
reviewed by:
Verified Customer
Western Blot Human 08/23/2012
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman
LotAB7
  5
reviewed by:
KAMESWARAN RAVICHANDRAN
Immunofluorescence Rat 08/22/2012
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Summary

ApplicationImmunofluorescence
Sample TestedRAT KIDNEY
SpeciesRat
LotF2
Western Blot HIF-1 alpha NB100-105
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5
reviewed by:
Verified Customer
Western Blot Human 08/22/2012
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Summary

ApplicationWestern Blot
Sample Testedhuman adrenal cancer cells (whole cell lysate)
SpeciesHuman
Western Blot HIF-1 alpha NB100-105
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5
reviewed by:
Verified Customer
Western Blot Mouse 05/15/2012
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Summary

ApplicationWestern Blot
Sample TestedCell lysate
SpeciesMouse
Western Blot HIF-1 alpha NB100-105
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4
reviewed by:
Verified Customer
Western Blot Human 05/11/2012
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Summary

ApplicationWestern Blot
Sample TestedHuman prostate cancer cells
SpeciesHuman
LotAB1
Western Blot HIF-1 alpha NB100-105
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4
reviewed by:
Huang Li-Ju
Western Blot Rat 12/14/2011
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Summary

ApplicationWestern Blot
Sample TestedRat liver
SpeciesRat
Western Blot HIF-1 alpha NB100-105
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4
reviewed by:
Hung-Kong Chen
Western Blot Mouse 12/09/2011
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Summary

ApplicationWestern Blot
Sample TestedMouse auditory cultured cell, Sample Amount: 30ug
SpeciesMouse
  4
reviewed by:
Shu-Wen Hsuan
Western Blot Human 11/21/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
  3
reviewed by:
Verified Customer
Immunocytochemistry Human 11/15/2011
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman tissues
SpeciesHuman
  4
reviewed by:
Qing Yang
Western Blot Human 09/30/2011
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Summary

ApplicationWestern Blot
Sample TestedMCF-7, Sample Amount: 100ug
SpeciesHuman
  4
reviewed by:
Gina Capley
Western Blot Rat 09/01/2010
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Summary

ApplicationWestern Blot
Sample Testedendothelial cell nuclear extract, Sample Amount: 40ug
SpeciesRat
  4
reviewed by:
Verified Customer
Western Blot Mouse 05/17/2010
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Summary

ApplicationWestern Blot
Sample Testedmouse cortical neurons, Sample Amount: 40ug
SpeciesMouse
LotAA1
  5
reviewed by:
Verified Customer
Western Blot 25037 04/13/2010
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Summary

ApplicationWestern Blot
Sample TestedHeLa cells
Species25037
LotN/A