HIF-1 alpha Antibody (H1alpha67)

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.

Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review.

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF-1 alpha in human kidney using NB100-105. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.

Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1 alpha antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine.

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF1alpha/PFKFB3 is upregulated in beta-cells of HIP rats and humans with type 2 diabetes. Representative Western blot of PFKFB3 and HIF1alpha levels in nuclear-enriched- and whole cell extracts from non-diabetic (ND) and T2D donor islets. Data are presented as mean +/- SEM, n = 3 independent biological samples for each group. Statistical significance was analyzed by Student t-test (*p < 0.05, ***p < 0.001) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10444-1) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFOA using 1 ug/mL of mouse anti-HIF-1 alpha monoclonal antibody NB100-105. Cells were stained using a donkey anti-rabbit secondary antibody and counterstained with DAPI (blue).

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the biotin conjugate of NB100-105. Staining of human glioblastoma multiforme.

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Upregulation of HIF-1alpha in human PCa. Protein expression of HIF-1alpha, VEGF, and GLUT4 were examined with western blot, in PC-3, DU145, and LNCaP cells after various treatments as indicated. Data are expressed as mean +/- SD of seven independent experiments. $p < 0.05 versus RWPE-1 or BPH1 cells or normal tissue. *p < 0.05 versus control group. #p < 0.05 versus si-HIF-1alpha or DDP group. Original blots are shown in Supplementary Figure 5. C: Ctrl; D: DDP; S: si-HIF-1alpha; D/S: DDP/si-HIF-1alpha. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-07973-4), licensed under a CC-BY licence.

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1alpha expression in PDAC and adjacent normal pancreatic tissue. PDAC with weak (intensity 1) nuclear and cytoplasmic HIF-1alpha staining (left). PDAC with strong (intensity 3) nuclear and moderate (intensity 2) cytoplasmic HIF-1alpha staining (right). Image collected and cropped by CiteAb from the following publication (//wjso.biomedcentral.com/articles/10.1186/s12957-018-1432-4), licensed under a CC-BY licence.

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis of HIF-1 alpha in human hepatocytes from cancer patient using anti-HIF-1 alpha antibody NB100-105. Image from verified customer review.

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - L. donovani infection induces HIF-1 alpha expression in CD11chi splenic DCs in an IRF-5 dependent manner.Mice were infected with 2x107 amastigotes intravenously. Immunoblot analysis of HIF-1 alpha expression in CD11c+ cells from C57BL/6 mice (upper panel) and densitometric analysis normalized to beta-actin expression and expressed as fold increase to results obtained with naive mice (lower panel). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1004938), licensed under a CC-BY licence.

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Upregulation of HIF-1alpha in human Pca. HIF-1alpha protein was detected by western blot in nonmalignant (RWPE-1 and BPH1) and PCa cell lines (PC-3, DU145, LNCaP, and 22RV1) as indicated. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-07973-4), licensed under a CC-BY licence.

Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in H929 cells using HIF-1 alpha antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Fe, Ma, Pm, Pm, Rb, Sh, Xp
• Applications: WB, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, LA, PLA, TCS, KO
• Clone: H1alpha67
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

HIF-1 alpha Antibody (H1alpha67) Summary

• Immunogen: This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
• Localization: HIF-1 is a nuclear protein
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HIF1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 1 - 5 ug/IP
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Gel Super Shift Assays 1:1-1:100
  • Immunoassay
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:20-1:50
  • Immunohistochemistry-Frozen 1:20-1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • Proximity Ligation Assay
  • Tissue Culture Substratum
  • Knockout Validated
  • Ligand Activation
• Application Notes: ChIP usage was reported in scientific literature. ELISA usage was reported in scientific literature (PMID: 20042684). Gel Super Shift Assays usage was reported in scientific literature (PMID: 22411794). Ligand Activation usage was reported in scientific literature (PMID: 26147748). Use in Immunoassay reported in scientific literature (PMID: 26147748). Use in vitro assay reported in multiple pieces of scientific literature. In WB, a band can be seen at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. Knock Out Validation was reported in scientific literature (PMID: 26861754). Use in proximity ligation assay reported in scientific literature (PMID 27595394). Use in Immunoblotting reported in multiple pieces of scientific literature. We recommend the use of a highly sensitive ECL reagent, such as West Pico PLUS, for Western blot detection.
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  COS-7 Nuclear Hypoxic Induced Cell Lysate
  HeLa CoCl2 treated/Untreated Cell Lysate
  HepG2 CoCl2 treated/Untreated Cell Lysate
  HeLa Hypoxic / Normoxic Cell Lysate
  HepG2 Hypoxic / Normoxic Cell Lysate
  HIF-1 alpha Knockout HeLa Cell Lysate

Reactivity Notes

Xenopus reactivity was reported in scientific literature (PMID: 18303027). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Canine reactivity reported in scientific literature (PMID: 24153191, 29974500). Feline reactivity reported in scientific literature (PMID: 30419801).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS with 1% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HIF-1 alpha Antibody (H1alpha67)

• AINT
• anti-HIF-1 alpha
• anti-HIF1A
• ARNT interacting protein
• ARNT-interacting protein
• Basic-helix-loop-helix-PAS protein MOP1
• BHLHE78
• Class E basic helix-loop-helix protein 78
• H1alpha67
• HIF 1A
• HIF1 alpha
• HIF-1 alpha
• HIF1
• HIF1A
• HIF-1a
• HIF-1alpha
• HIF-1-alpha
• HIF1-alpha
• hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha
• hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• hypoxia-inducible factor 1-alpha
• Member of PAS protein 1
• member of PAS superfamily 1
• MOP1
• PAS domain-containing protein 8
• PASD8

Background

Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease (1). In cancer and particularly solid tumors, hypoxia plays a critical role in the regulation of genes involved in stem cell renewal, epithelial to mesenchymal transition (EMT), metastasis and angiogenesis. In the tumor microenvironment (TME), hypoxia influences the properties and function of stromal cells (e.g., fibroblasts, endothelial and immune cells) and is a strong determinant of tumor progression (2,3).

HIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. In its active form under hypoxic conditions, HIF-1 is stabilized by the formation of a heterodimer of HIF-1 alpha and ARNT/HIF-1 beta subunits. Nuclear HIF-1 engages p300/CBP for binding to hypoxic response elements (HREs). This process induces transcription and regulation of genes including EPO, VEGF, iNOS2, ANGPT1 and OCT4 (4,5).

Under normoxic conditions, the HIF-1 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system. This process is mediated by prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-402 and Pro-564) within the oxygen-dependent degradation domain of HIF-1 alpha. Once hydroxylated, HIF-1 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (4). pVHL dependent regulation of HIF-1 alpha plays a role in normal physiology and disease states. Regulation of HIF-1 alpha by pVHL is critical for the suppressive function of Foxp3+ regulatory Tcells (6). Repression of pVHL expression in chronic lymphocytic leukemia (CLL) B cells leads to HIF-1 alpha stabilization and increased VEGF secretion (7).

References

1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.

2. Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413

3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y

4. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483

5. Dengler, V. L., Galbraith, M. D., & Espinosa, J. M. (2014). Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology. https://doi.org/10.3109/10409238.2013.838205

6. Lee, J. H., Elly, C., Park, Y., & Liu, Y. C. (2015). E3Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1 alpha to Maintain Regulatory T Cell Stability and Suppressive Capacity. Immunity. https://doi.org/10.1016/j.immuni.2015.05.016

7. Ghosh, A. K., Shanafelt, T. D., Cimmino, A., Taccioli, C., Volinia, S., Liu, C. G.,... Kay, N. E. (2009). Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood. https://doi.org/10.1182/blood-2008-10-185686

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIF-1 alpha Antibody (NB100-105)(835)

Publications using NB100-105

• Kondrashina, A. A Phosphorescent Nanoparticle-Based Probe for Sensing and Imaging of (Intra)Cellular Oxygen in Multiple Detection Modalities Adv Funct Mater 2012 (ICC/IF, Rat)
• Chen L, Wang Z, Xu Q et Al. The failure of DAC to induce OCT2 expression and its remission by hemoglobin-based nanocarriers under hypoxia in renal cell carcinoma Theranostics Feb 18 2020 [PMID: 32206108] (WB, ChIP, Mouse, Human)
• Veerman KJ. Investigating the Pro-Atherogenic Potential of Chronic Hyperglycemia: Is Diabetic Atherosclerosis a Microvascular Complication? [Internet]. 2011
• Zhang W, Yu L, Han X et al. The secretome of human dental pulp stem cells protects myoblasts from hypoxia induced injury via the Wnt/beta catenin pathway Int. J. Mol. Med. Mar 4 2020 [PMID: 32323739] (WB, Mouse)
• Weng ML, Chen WK, Chen XY et al. Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1alpha pathway suppression Nat Commun Apr 20 2020 [PMID: 32313017] (WB, Human, Mouse)
• Imanishi M, Yamakawa Y, Fukushima K et al. Fibroblast-specific ERK5 deficiency changes tumor vasculature and exacerbates tumor progression in a mouse model Naunyn Schmiedebergs Arch. Pharmacol. Apr 19 2020 [PMID: 32307577]
• Qiao S, Koh SB, Vivekanandan V et al. REDD1 loss reprograms lipid metabolism to drive progression of RAS mutant tumors Genes Dev. Apr 9 2020 [PMID: 32273287] (WB, Mouse)
• Wang C, Luo J, Chen Z et Al. MiR-375 Impairs the Invasive Capabilities of Hepatoma Cells by Targeting HIF1 alpha Under Hypoxia Dig. Dis. Sci. Mar 25 2020 [PMID: 32215815]
• Okada K, Mori D, Makii Y et Al. Hypoxia-inducible factor-1 alpha maintains mouse articular cartilage through suppression of NF- kappa B signaling Sci Rep Mar 25 2020 [PMID: 32214220] (WB, IHC, Mouse)
• Tang Sy, Liu Dx, Li Y et Al. Caspr1 Facilitates sAPP alpha Production by Regulating alpha -Secretase ADAM9 in Brain Endothelial Cells Front Mol Neurosci Mar 6 2020 [PMID: 32210761]
• Sharma A, Kilari S, Cai C et Al. Increased fibrotic signaling in a murine model for intra-arterial contrast induced acute kidney Injury (CI-AKI) Am. J. Physiol. Renal Physiol. Mar 23 2020 [PMID: 32200666] (IHC, Mouse)
• Pei X, Kim H, Lee M et al. Local delivery of cardiac stem cells overexpressing HIF-1 alpha promotes angiogenesis and muscular tissue repair in a hind limb ischemia model J Control Release Mar 16 2020 [PMID: 32194175] (WB, Rat)
• Barbosa DM, Fahlbusch P, Herzfeld de Wiza D et al. Rhein, a novel Histone Deacetylase (HDAC) inhibitor with antifibrotic potency in human myocardial fibrosis Sci Rep Mar 17 2020 [PMID: 32184434] (WB, Human)
• Dong Z, Li R, Xu L et al. Histone Hyperacetylation Mediates Enhanced IL-1 beta Production in LPS/IFN-gamma stimulated Macrophages Immunology Feb 15 2020 [PMID: 32061096]
• Li J, Zhou W, Chen W et al. Mechanism of the hypoxia inducible factor 1/hypoxic response element pathway in rat myocardial ischemia/diazoxide post conditioning Mol Med Report Jan 28 2020 [PMID: 32016463] (WB, Rat)
• Chen R Hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors induce autophagy and have a protective effect in an in-vitro ischaemia model Scientific Reports Jan 31 2020 [PMID: 32005890] (WB, Rat)
• Rajendran G, Schonfeld MP, Tiwari R et al. Inhibition of Endothelial PHD2 Suppresses Post-Ischemic Kidney Inflammation through Hypoxia-Inducible Factor-1 J. Am. Soc. Nephrol. Jan 29 2020 [PMID: 31996410] (IHC-Fr)
• Karshovska E, Wei Y, Subramanian P et al. HIF-1alpha (Hypoxia-Inducible Factor-1alpha) Promotes Macrophage Necroptosis by Regulating miR-210 and miR-383 Arterioscler. Thromb. Vasc. Biol. Jan 30 2020 [PMID: 31996026] (IHC, Mouse)
• Su CC, Hsu TC, Hsiao CH et al. Effects of antibodies against human parvovirus B19 on angiogenic signaling Mol Med Rep Jan 9 2020 [PMID: 31922220]
• Roudkenar MH, Fukumoto M, Roushandeh AM et al. Disturbance in the regulation of miR 17-92 cluster on HIF-1 alpha expression contributes to clinically relevant radioresistant cells: an in vitro study Cytotechnology Jan 8 2020 [PMID: 31916114]
• Torigoe K, Obata Y, Torigoe M et al. Hexapeptide derived from prothymosin alpha attenuates cisplatin-induced acute kidney injury Clin. Exp. Nephrol. Jan 7 2020 [PMID: 31912273]
• Yoo HC, Park SJ, Nam M et al. A Variant of SLC1A5 Is a Mitochondrial Glutamine Transporter for Metabolic Reprogramming in Cancer Cells Cell Metab. Dec 13 2019 [PMID: 31866442] (Human)
• Robinson T. Targeting cFLIP to Inhibit Residual Cancer Stem Cells after Chemotherapy. Thesis 2017 (WB)
• Li H. Role of the p53/miR-34a pathway in the response to tumor hypoxia Thesis 2017 (WB, ChIP)
• Sasabe E, Tomomura A, Hamada M. Nitric Oxide Attenuates Hypoxia-Induced 5-FU Resistance of Oral Squamous Cell Carcinoma Cells International Journal ofCancer and Clinical Research 2015 (IHC-P, Human)
• Vorrink SU. Regulatory crosstalk and interference between the PCB 126 stimulated AHR and hypoxia stimulated HIF-1a signaling pathways. 2014 (IHC-P, Rat)
  
  Details:
  Mouse monoclonal anti-HIF-1 alpha antibody used for IHC on paraffin-embedded livers from untreated and PCB 126 treated rats at a dilution of 1:50, incubated for 2 hours at room temperature (Figure 3-1)
• Pezzuto A, Perrone G, Orlando N, Citarella F A close relationship between HIF-1 alpha expression and bone metastases in advanced NSCLC, a retrospective analysis Oncotarget Dec 17 2019 [PMID: 31903166] (IHC-P, Human)
• Aguanno S, Petrelli C, Di Siena S et al. A Three-Dimensional Culture Model of Reversibly Quiescent Myogenic Cells Stem Cells International Nov 11 2019 [PMID: 31827532] (WB, Mouse)
• Li S, Lin Q, Shao X, et al. Drp1-regulated PARK2-dependent mitophagy protects against renal fibrosis in unilateral ureteral obstruction Free Radic. Biol. Med. Dec 9 2019 [PMID: 31825802] (WB, Mouse)
• Schultz E, Jones T, Clamp H, Barr K Glycaemic control in diabetic rats treated with islet transplantation using plasma combined with hydroxypropylmethyl cellulose hydrogel Acta Biomater Dec 4 2019 [PMID: 31811957] (WB, Rat)
• Madsen PM, Desu HL, Pablo de Rivero Vaccari J et al. Oligodendrocytes modulate the immune-inflammatory response in EAE via TNFR2 signaling Brain Behav. Immun. Nov 27 2019 [PMID: 31785393] (IHC, Mouse)
• Ghezzi C, Wong A, Chen BY et al. A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells Nat Commun Nov 29 2019 [PMID: 31784510] (WB, Human)
• Bommi PV, Chand V, Mukhopadhyay NK et al. NER-factor DDB2 regulates HIF1a and hypoxia-response genes in HNSCC Oncogene Nov 18 2019 [PMID: 31740787] (WB, Human)
• Kang YBA, Eo J, Bulutoglu B et al. Progressive hypoxia-on-a-chip: An in vitro oxygen gradient model for capturing the effects of hypoxia on primary hepatocytes in health and disease Biotechnol. Bioeng. Nov 18 2019 [PMID: 31736056] (ICC/IF, Rat)
• Wu JY, Huang TW, Hsieh YT et al. Cancer-Derived Succinate Promotes Macrophage Polarization and Cancer Metastasis via Succinate Receptor Mol. Cell Nov 5 2019 [PMID: 31735641]
• Courtney KD, Ma Y, Diaz de Leon A et al. HIF-2 Complex Dissociation, Target Inhibition, and Acquired Resistance with PT2385, a First-in-Class HIF-2 Inhibitor in Clear Cell Renal Cell Carcinoma Patients Clin. Cancer Res. Nov 14 2019 [PMID: 31727677] (PLA, Human)
• Romeo-Guitart D, Leiva-Rodriguez T, Fores J, Casas C Improved Motor Nerve Regeneration by SIRT1/Hif1 alpha-Mediated Autophagy Cells Oct 30 2019 [PMID: 31671642] (IHC, Rat)
• Zhang D, Tang Z, Huang H et al. Metabolic regulation of gene expression by histone lactylation Nature 2019 Oct 01 [PMID: 31645732]
• Terabe K, Ohashi Y, Tsuchiya S et al. Chondroprotective effects of 4-methylumbelliferone and hyaluronan synthase-2 overexpression involve changes in chondrocyte energy metabolism J. Biol. Chem. Oct 16 2019 [PMID: 31619518] (Bovine)
• Ji Cho M, Yoon SJ, Kim W et al. Oxidative stress-mediated TXNIP loss causes RPE dysfunction Exp. Mol. Med. 2019 Oct 15 [PMID: 31615975] (WB, Human)
• Han S, Huang T, Hou F et al. The prognostic value of hypoxia-inducible factor-1 alpha in advanced cancer survivors: a meta-analysis with trial sequential analysis Ther Adv Med Oncol 2019 Sep 24 [PMID: 31579115] (IHC-P, Human)
• Han S, Huang T, Hou F et al. The prognostic value of hypoxia-inducible factor-1 alpha in advanced cancer survivors: a meta-analysis with trial sequential analysis. Ther Adv Med Oncol. 2019 Sep 24 [PMID: 31579115] (IHC-P, Human)
• Allon I, Vered M, Kaplan I et al. Rare variants of head and neck squamous cell carcinoma -differential immunohistochemical profiles Acta Histochem. Sep 20 2019 [PMID: 31548087] (IHC-P, Human)
• Kim S, Jo CH, Kim GH Effects of empagliflozin on nondiabetic salt-sensitive hypertension in uninephrectomized rats Hypertens. Res. Sep 19 2019 [PMID: 31537914] (WB, Rat)
• Ben-Aicha S, Escate R, Casani L et al. HDL remodelled in hypercholesterolemic blood induce epigenetically driven downregulation of endothelial HIF-1 alpha expression in a preclinical animal model Cardiovasc. Res. Aug 30 2019 [PMID: 31504272] (IHC/IF, Porcine)
• David BT, Curtin JJ, Goldberg DC et al. Hypoxia Inducible Factor 1 alpha (HIF-1 alpha) counteracts the acute death of cells transplanted into the injured spinal cord eNeuro [PMID: 31488552] (WB)
• Soyal SM, Bonova P, Kwik M et al. The Expression of CNS-Specific PPARGC1A Transcripts Is Regulated by Hypoxia and a Variable GT Repeat Polymorphism Mol. Neurobiol. Aug 30 2019 [PMID: 31471878] (WB, Human)
• Robinson CM, Poon BPK, Kano Y et al. A hypoxia-inducible HIF1-GAL3ST1-sulfatide axis enhances ccRCC immune evasion via increased tumor cell-platelet binding Mol. Cancer Res. Aug 19 2019 [PMID: 31427440] (ChIP, Human)
• Bacci L, Aiello A, Ripoli C et al. H19-Dependent Transcriptional Regulation of beta 3 and beta 4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer Int J Mol Sci Aug 17 2019 [PMID: 31426484] (ChIP, WB)
• Wang Y, Wang B, Qi X et al. Resveratrol Protects Against Post-Contrast Acute Kidney Injury in Rabbits With Diabetic Nephropathy Front Pharmacol Jul 26 2019 [PMID: 31402864] (WB, Rabbit)
• Qi D, Wei M, Jiao S et al. Hypoxia inducible factor 1 alpha in vascular smooth muscle cells promotes angiotensin II-induced vascular remodeling via activation of CCL7-mediated macrophage recruitment Cell Death Dis Jul 18 2019 [PMID: 31320613] (WB, ChIP, Mouse)
• Wang Y, Zhang X, Hua Z et al. Blood Oxygen Level-Dependent Imaging and Intravoxel Incoherent Motion MRI of Early Contrast-Induced Acute Kidney Injury in a Rabbit Model Kidney Blood Press. Res. Jun 28 2019 [PMID: 31256149] (IHC, Rabbit)
• Montemurro C, Nomoto H, Pei L et al. IAPP toxicity activates HIF1 alpha/PFKFB3 signaling delaying beta-cell loss at the expense of beta-cell function Nat Commun Jun 18 2019 [PMID: 31213603] (WB, Rat)
• Ock S, Park S, Lee J et al. RANKL blockade suppresses pathological angiogenesis and vascular leakage in ischemic retinopathy Biochem. Biophys. Res. Commun. Jun 14 2019 [PMID: 31208720] (WB, Human)
• Hu M, Chen X, Ma L et al. AMPK Inhibition Suppresses the Malignant Phenotype of Pancreatic Cancer Cells in Part by Attenuating Aerobic Glycolysis J Cancer. 2019 Apr 20 [PMID: 31205544] (WB, Mouse)
• Maugeri G, D'Amico AG, Federico C et al. Involvement of A3 Adenosine Receptor in Neuroblastoma Progression via Modulation of the Hypoxic/Angiogenic Pathway J. Mol. Neurosci. Jun 5 2019 [PMID: 31166001] (WB, Human)
• Xian Y, Chen Z, Deng H et al. Exenatide mitigates inflammation and hypoxia along with improved angiogenesis in obese fat tissue J. Endocrinol. May 1 2019 [PMID: 31137012] (Mouse)
• Singh Santosh Kumar, Mishra Manoj Kumar, Singh Rajesh et al. Hypoxia-inducible factor-1 alpha induces CX3CR1 expression and promotes the epithelial to mesenchymal transition (EMT) in ovarian cancer cells. Journal of Ovarian Research 2019 May 10 [PMID: 31077234] (IHC, Human)
• Wang Z and Ren K Evaluation of iodine contrast-induced acute kidney injury via different injection routes using BOLD-MRI Ren Fail Nov 1 2019 [PMID: 31057054] (IHC-P, Rabbit)
• Fujimoto TN, Colbert LE, Huang Y et al. Selective EGLN Inhibition Enables Ablative Radiotherapy and Improves Survival in Unresectable Pancreatic Cancer Cancer Res. May 1 2019 [PMID: 31043430] (WB, Mouse)
• Cheng CC, Chi PL, Shen MC et al. Caffeic Acid Phenethyl Ester Rescues Pulmonary Arterial Hypertension through the Inhibition of AKT/ERK-Dependent PDGF/HIF-1alpha In Vitro and In Vivo Int J Mol Sci Mar 22 2019 [PMID: 30909527] (WB, Rat)
• Cai C, Yang B, Kilari S et al. Evaluation of Venous Stenosis Angioplasty in a Murine Arteriovenous Fistula Model J Vasc Interv Radiol Mar 19 2019 [PMID: 30902494] (IHC-P, Mouse)
• Kengelbach-Weigand A, Lotz L, Schmid R et al. Intra- and Postoperative Blood Flow Monitoring in a Sheep Model of Uterus Transplantation In Vivo Jan 3 2019 [PMID: 30804109] (IHC, Sheep)
• Zhang F, Li R, Yang Y et al. Specific Decrease in B-Cell-Derived Extracellular Vesicles Enhances Post-Chemotherapeutic CD8+ T Cell Responses Immunity Feb 6 2019 [PMID: 30770248] (WB, Mouse)
• Hong B, Zhou J, Ma K et al. TRIB3 Promotes the Proliferation and Invasion of Renal Cell Carcinoma Cells via Activating MAPK Signaling Pathway. Int. J. Biol. Sci. Jan 8 2019 [PMID: 30745845] (WB, Human)
• Li HS, Zhou YN, Li L et al. HIF-1a protects against oxidative stress by directly targeting mitochondria. Redox Biol Jan 14 2019 [PMID: 30686776] (WB, Human)
• Williams PA, Braine CE, Kizhatil K et al. Inhibition of monocyte-like cell extravasation protects from neurodegeneration in DBA/2J glaucoma. Mol Neurodegener Jan 22 2019 [PMID: 30670050] (ICC/IF, Mouse)
• Guo L, Fernandez R, Sakamoto A et al. Vascular Permeability Assay in Human Coronary and Mouse Brachiocephalic Arteries. Stem Cells International. Nov 11 2018 [PMID: 30613761]
• Laurent V, Toulet A, Attane C et al. Periprostatic adipose tissue favors prostate cancer cell invasion in an obesity- dependent manner: role of oxidative stress. Mol. Cancer Res. Jan 3 2019 [PMID: 30606769] (IHC, Human)
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• Stei MM, Loeffler KU, Kurts C et al. Impact of macrophages on tumor growth characteristics in a murine ocular tumor model Exp. Eye Res. 2016 Jul 15 [PMID: 27426931] (IHC, Human)
• Hnatiuk AP, Ong SG, Olea FD et al. Allogeneic Mesenchymal Stromal Cells Overexpressing Mutant Human Hypoxia-Inducible Factor 1-alpha (HIF1-alpha) in an Ovine Model of Acute Myocardial Infarction. J Am Heart Assoc 2016 Jul 06 [PMID: 27385426] (WB)
• Yoon C, Chang KK, Lee JH et al. Multimodal targeting of tumor vasculature and cancer stem-like cells in sarcomas with VEGF-A inhibition, HIF-1a inhibition, and hypoxia-activated chemotherapy Oncotarget Jul 12 2016 [PMID: 27374091] (WB, Mouse)
• Schokrpur S, Hu J, Moughon DL et al. CRISPR-Mediated VHL Knockout Generates an Improved Model for Metastatic Renal Cell Carcinoma Sci Rep 2016 Jun 30 [PMID: 27358011] (WB, ICC/IF)
• Sanagawa A, Iwaki S, Asai M et al. Sphingosine 1phosphate induced by hypoxia increases the expression of PAI1 in HepG2 cells via HIF1a Mol Med Rep 2016 Aug 01 [PMID: 27357063]
• Ichimaru S, Nakagawa S, Arai Y et al. Hypoxia Potentiates Anabolic Effects of Exogenous Hyaluronic Acid in Rat Articular Cartilage. Int J Mol Sci 2016 Jun 25 [PMID: 27347945] (WB)
• Maugeri G, Grazia D'Amico A, Reitano R et al. PACAP and VIP Inhibit the Invasiveness of Glioblastoma Cells Exposed to Hypoxia through the Regulation of HIFs and EGFR Expression. Front Pharmacol 2016 May 31 [PMID: 27303300] (WB)
• Hoefflin R, Lahrmann B, Warsow G et al. Spatial niche formation but not malignant progression is a driving force for intratumoural heterogeneity. Nat Commun Jun 13 2016 [PMID: 27291893] (IHC)
• Tan J, Prosser H, Dunn L et al. High-Density Lipoproteins Rescue Diabetes-Impaired Angiogenesis via Scavenger Receptor Class B Type I. Diabetes Jul 8 1905 [PMID: 27284113]
• Hicks KC, Patel TB. Sprouty 2 Protein Regulates Hypoxia-inducible Factor-alpha (HIF1-alpha) Protein Levels and Transcription of HIF1- alpha responsive Genes J. Biol. Chem. 2016 Aug 05 [PMID: 27281823] (IP, Human)
• Samanta D, Park Y, Andrabi SA et al. PHGDH Expression is Required for Mitochondrial Redox Homeostasis, Breast Cancer Stem Cell Maintenance and Lung Metastasis. Cancer Res. 2016 Jun 8. [PMID: 27280394] (WB, Human)
• Lo Dico A, Costa V, Martelli C et al. MiR675-5p Acts on HIF-1 alpha to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma. Theranostics Jun 9 2016 [PMID: 27279905] (ICC/IF, IHC-P)
• Fecher RA, Horwath MC, Friedrich D et al. Inverse Correlation between IL-10 and HIF-1a in Macrophages Infected with Histoplasma capsulatum. J. Immunol. Jun 6 2016 [PMID: 27271565] (WB, Mouse)
• Wert Katherine J, Mahajan Vinit B, Zhang Lijuan et al. Neuroretinal hypoxic signaling in a new preclinical murine model for prolifeRative diabetic retinopathy. Signal Transduction and Targeted Therapy 2016 [PMID: 27195131] (WB, Mouse)
• Badar T, Handisides DR, Benito JM et al. A Phase I Study of Evofosfamide, an Investigational Hypoxia-Activated Prodrug, in Patients with Advanced Leukemia. Am. J. Hematol. May 11 2016 [PMID: 27169385] (IHC-P, Human)
• Bellanti F, Mirabella L, Mitarotonda D et al. Propofol but not sevoflurane prevents mitochondrial dysfunction and oxidative stress by limiting HIF-1alpha activation in hepatic ischemia/reperfusion injury. Free Radic. Biol. Med. May 3 2016 [PMID: 27154980]
• Ma J, Mi C, Wang KS et al. Zinc finger protein 91 (ZFP91) activates HIF-1alpha via NF-kB/p65 to promote proliferation and tumorigenesis of colon cancer. Oncotarget. Jun 14 2016 [PMID: 27144516] (Human)
• Truong AS, Lockett MR. Oxygen as a chemoattractant: confirming cellular hypoxia in paper-based invasion assays. Analyst. 2016 May 3 [PMID: 27138213] (ICC/IF, Human)
  
  Details:
  This citation used the DyLight 550 version of this antibody.
• Guinot A, Lehmann H, Wild PJ, Frew IJ. Combined deletion of Vhl, Trp53 and kif3a causes cystic and neoplastic renal lesions. J. Pathol. Apr 29 2016 [PMID: 27126173] (IHC, ICC/IF, Mouse)
• Mongiardi MP, Savino M, Falchetti ML et al. c-MYC inhibition impairs hypoxia response in glioblastoma multiforme. Oncotarget. May 31 2016 [PMID: 27119353] (Human)
• Luo B, Wang M, Hou N et al. ATP-Dependent Lon Protease Contributes to Helicobacter pylori-Induced Gastric Carcinogenesis. Neoplasia. 2016 Apr 01 [PMID: 27108387] (WB, Human)
• Kowalik MA, Guzzo G, Morandi A et al. Metabolic reprogramming identifies the most aggressive lesions at early phases of hepatic carcinogenesis. Oncotarget. May 31 2016 [PMID: 27070090] (Rat)
• Park SJ, Kim JG, Kim ND et al. Estradiol, TGF-beta1 and hypoxia promote breast cancer stemness and EMT-mediated breast cancer migration. Oncol Lett 2016 Mar [PMID: 26998096] (WB)
• Ananthula S, Sinha A, El Gassim M et al. Geminin overexpression-dependent recruitment and crosstalk with mesenchymal stem cells enhance aggressiveness in triple negative breast cancers. Oncotarget 2016 Apr 12 [PMID: 26989079] (WB)
• He Z, Jiang X, Huang X et al. Folic Acid Represses Hypoxia-Induced Inflammation in THP-1 Cells through Inhibition of the PI3K/Akt/HIF-1a Pathway. Int J Clin Exp Med Jul 30 2016 [PMID: 26974319]
• Zhang X, Liang D, Fan J et al. Zinc Attenuates Tubulointerstitial Fibrosis in Diabetic Nephropathy Via Inhibition of HIF Through PI-3K Signaling. Biol Trace Elem Res 2016 Oct [PMID: 26956696] (Mouse)
• Karuppagounder SS, Alim I, Khim SJ et al. Therapeutic targeting of oxygen-sensing prolyl hydroxylases abrogates ATF4-dependent neuronal death and improves outcomes after brain hemorrhage in several rodent models Sci Transl Med. 2016 Mar 01 [PMID: 26936506] (WB, Rat)
• Kubo H, Kitajima Y, Kai K et al. Regulation and clinical significance of the hypoxia-induced expression of ANGPTL4 in gastric cancer. Oncol Lett 2016 Feb [PMID: 26893686] (WB)
• Chen H, Jia P, Kang H et al. Upregulating Hif-1alpha by Hydrogel Nanofibrous Scaffolds for Rapidly Recruiting Angiogenesis Relative Cells in Diabetic Wound. Adv Healthc Mater 2016 Apr 20 [PMID: 26891197]
• Fujimoto Y, Urashima T, Shimura D et al. Low Cardiac Output Leads Hepatic Fibrosis in Right Heart Failure Model Rats. PLoS One 2016 Feb 10 [PMID: 26863419] (WB)
• Mori H, Yao Y, Learman BS et al. Induction of WNT11 by hypoxia and hypoxia-inducible factor-1alpha regulates cell proliferation, migration and invasion. Sci Rep. 2016 Feb 10 [PMID: 26861754] (WB, Human)
• Mazar J, Qi F, Lee B et al. miR-211 functions as a metabolic switch in human melanoma cells. Mol. Cell. Biol. 2016 Jan 19 [PMID: 26787841] (WB, Human)
• Morita K, Shirabe K, Taketomi A et al. Relevance of microRNA-18a and microRNA-199a-5p to hepatocellular carcinoma recurrence after living donor liver transplantation. Liver Transpl 2016 May [PMID: 26783726]
• Ai F, Chen M, Li W et al. Danshen improves damaged cardiac angiogenesis and cardiac function induced by myocardial infarction by modulating HIF1alpha/VEGFA signaling pathway. Int J Clin Exp Med 2015 [PMID: 26770433]
• Chen K, Zeng J, Xiao H et al. Regulation of glucose metabolism by p62/SQSTM1 through HIF1alpha. J. Cell. Sci. 2016 Jan 07 [PMID: 26743088] (WB, Human)
  
  Details:
  HIF-1 alpha (clone H1alpha67) was used at 1:500 dilution for WB assays in experiments involving human kidney cell lines namely ACHN, Caki-1 and HEK293T.
• Maugeri G, D'Amico AG, Reitano R et al. Parkin modulates expression of HIF-1alpha and HIF-3alpha during hypoxia in gliobastoma-derived cell lines in vitro. Cell Tissue Res. 2016 Jan 08 [PMID: 26742768] (WB, ICC/IF, Human)
  
  Details:
  HIF-1 alpha (clone H1alpha67) was used at 1:500 dilution for WB assays in lysates from human glioblastoma cell lines U87MG and T98G which were left untransfected or transfected with empty vector (Control shRNA) or with Parkin shRNA (Parkin shRNA) followed by culturing under normoxia or hypoxia conditions. The antibody was used for immunofluorescence analysis (ICC/IF) also on the same cells under similar experimental conditions.
• Lee SB, Frattini V, Bansal M et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016 Jan 14 [PMID: 26735018] (WB, Human)
• Nishi H, Sasaki T, Nagamitsu Y et al. Hypoxia inducible factor-1 mediates upregulation of urokinase-type plasminogen activator receptor gene transcription during hypoxia in cervical cancer cells. Oncol Rep 2016 Feb [PMID: 26718775]
• Danhier P, Krishnamachary B, Bharti S et al. Combining Optical Reporter Proteins with Different Half-lives to Detect Temporal Evolution of Hypoxia and Reoxygenation in Tumors. Neoplasia. 2015 Dec 01 [PMID: 26696369] (WB, Human)
• Sharpe MA, Baskin DS. Monoamine oxidase B levels are highly expressed in human gliomas and are correlated with the expression of HiF-1alpha and with transcription factors Sp1 and Sp3. Oncotarget 2016 Jan 19 [PMID: 26689994] (IHC)
• Mousavizadeh R, Scott A, Lu A et al. Angiopoietin-like 4 promotes angiogenesis in the tendon and is increased in cyclically loaded tendon fibroblasts J. Physiol. (Lond.) Jun 1 2016 [PMID: 26670924] (WB)
• Yamano S, Gi M, Tago Y et al. Role of deltaNp63(pos)CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas. Cancer Sci 2016 Feb [PMID: 26663681] (IHC)
• Tu Tran Cam, Nagano Masumi, Yamashita Toshiharu et al. A Chemokine Receptor, CXCR4, Which Is Regulated by Hypoxia-Inducible Factor 2a, Is Crucial for Functional Endothelial Progenitor Cells MigRation to Ischemic Tissue and Wound Repair. Stem Cells and Development 2016 [PMID: 26620723] (WB, ChIP, Mouse)
• Aiken J, Roudier E, Ciccone J et al. Phosphorylation of murine double minute-2 on Ser166 is downstream of VEGF-A in exercised skeletal muscle and regulates primary endothelial cell migration and FoxO gene expression. FASEB J. 2015 Nov 17 [PMID: 26578686] (WB, Human, Mouse, Rat)
• Clancy KW, Weerapana E, Thompson PR Detection and identification of protein citrullination in complex biological systems. Curr Opin Chem Biol 2016 Feb 30 [PMID: 26517730]
• Cho MH, Park CK, Park M et al. Clinicopathologic Features and Molecular Characteristics of Glucose Metabolism Contributing to 18F-fluorodeoxyglucose Uptake in Gastrointestinal Stromal Tumors. PLoS ONE. 2015 Oct 29 [PMID: 26509967] (IHC-P, Human)
• Zhang L, Takara K, Yamakawa D et al. Apelin as a marker for monitoring the tumor vessel normalization window during antiangiogenic therapy. Cancer Sci. 2015 Oct 17 [PMID: 26475217] (WB, Mouse)
• Kwang Kim K, Abelman S, Yano N et al. Tetrathiomolybdate inhibits mitochondrial complex IV and mediates degradation of hypoxia-inducible factor-1a in cancer cells. Sci Rep. 2015 Oct 16 [PMID: 26469226] (WB, Human)
• Cui H, Qin Q, Yang M et al. Bortezomib enhances the radiosensitivity of hypoxic cervical cancer cells by inhibiting HIF-1a expression. Int J Clin Exp Pathol 2015 Aug 15 [PMID: 26464645] (WB, Human)
• Abe T, Kitaoka Y, Kikuchi DM et al. High-intensity interval training-induced metabolic adaptation coupled with an increase in Hif-1alpha and glycolytic protein expression. J Appl Physiol (1985) 2015 Dec 1 [PMID: 26429867]
• de la Puente P, Muz B, Gilson RC et al 3D tissue-engineered bone marrow as a novel model to study pathophysiology and drug resistance in multiple myeloma. Biomaterials. 2015 Dec [PMID: 26402156] (IHC-P, Human)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Yu MO, Park KJ, Park DH et al. Reactive oxygen species production has a critical role in hypoxia-induced Stat3 activation and angiogenesis in human glioblastoma. J. Neurooncol. 2015 Aug 22 [PMID: 26297045] (WB, Human)
  
  Details:
  HIF1 alpha antibody was used for WB on lysates of human glioblastoma cell line U87 subjected to growth in vitro under hypoxic conditions (Fig. 1A).
• Wong CS, Chen A, Liu MC et al. Loss of Siah2 does not impact angiogenic potential of murine endothelial cells. Microvasc. Res. 2015 Aug 11 [PMID: 26275748] (WB, Mouse)
  
  Details:
  HIF 1-alpha antibody was used for WB on lysates of endothelial cells (obtained from WT and Siah2?/? mice lungs) that were cultured under normoxia and hypoxia conditions (Fig. 1A).
• Frey JL, Stonko DP, Faugere MC, Riddle RC Hypoxia-inducible factor-1alpha restricts the anabolic actions of parathyroid hormone. Bone Res 2014 May 13 [PMID: 26273518] (IP)
• Donato MD, Fanelli M, Mariani M et al. Nek6 and Hif-1alpha cooperate with the cytoskeletal gateway of drug resistance to drive outcome in serous ovarian cancer. Am J Cancer Res 2015 [PMID: 26269749] (IP)
• Belo AI, van Vliet SJ, Maus A et al. Hypoxia inducible factor 1alpha down regulates cell surface expression of alpha1,2-fucosylated glycans in human pancreatic adenocarcinoma cells. FEBS Lett. 2015 Aug 19 [PMID: 26232512] (ICC/IF, Human)
• Wang IK, Sun KT, Tsai TH et al. MiR-20a-5p mediates hypoxia-induced autophagy by targeting ATG16L1 in ischemic kidney injury Life Sci. 2015 Jul 10 [PMID: 26165754] (WB, Human)
• Chan MC, Atasoylu O, Hodson E et al. Potent and Selective Triazole-Based Inhibitors of the Hypoxia-Inducible Factor Prolyl-Hydroxylases with Activity in the Murine Brain PLoS ONE. 2015 Jul 07 [PMID: 26147748] (IA, Human)
  
  Details:
  HIF1 alpha antibody was used for auantitative HIF1 alpha meso scale discovery immunoassay on experiments involving RCC4, HeLa, Hep3B, MCF-7 and U2OS cell lines.
• Nakatani K, Watabe T, Masuda D et al. Myocardial energy provision is preserved by increased utilization of glucose and ketone bodies in CD36 knockout mice Metab. Clin. Exp. 2015 Jun 06 [PMID: 26130608] (WB, Mouse)
  
  Details:
  HIF1 alpha antibody was used for WB on protein extraction from cardiac tissues of WT and CD-36 knock-out mice (Fig. 2G).
• Lee JH, Elly C, Park Y, Liu YC. E3 Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1a to Maintain Regulatory T Cell Stability and Suppressive Capacity Immunity. 2015 Jun 16 [PMID: 26084024] (ChIP, Mouse)
• Bohuslavova R, Skvorova L, Cerychova R, Pavlinkova G. Gene expression profiling of changes induced by maternal diabetes in the embryonic heart. Reprod Toxicol 2015 Nov [PMID: 26073000]
• Hammami A, Charpentier T, Smans M, Stager S. IRF-5-Mediated Inflammation Limits CD8+ T Cell Expansion by Inducing HIF-1aand Impairing Dendritic Cell Functions during Leishmania Infection PLoS Pathog. 2015 Jun 01 [PMID: 26046638] (WB, Mouse)
• Espana-Agusti J, Tuveson DA, Adams DJ, Matakidou A. A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules. Sci Rep 2015 [PMID: 26046460] (IHC, Mouse)
• Fan Y, Wang L, Liu C et al. Local renin-angiotensin system regulates hypoxia-induced vascular endothelial growth factor synthesis in mesenchymal stem cells. Int J Clin Exp Pathol. 2015 Jun 05 [PMID: 26045756] (WB, Rat)
• Sun L, Xu C, Chen G et al. A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway. Int. J. Biol. Sci. 2015 May 22 [PMID: 25999789] (WB, Human)
• Joo HY, Yun M, Jeong J et al. SIRT1 deacetylates and stabilizes hypoxia-inducible factor-1alpha (HIF-1alpha) via direct interactions during hypoxia. Biochem. Biophys. Res. Commun. 2015 May 13 [PMID: 25979359] (IP, WB, Human)
• Zhang Z, Pang X, Tang Z et al. Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep 2015 May 14 [PMID: 25975603]
• Babu E, Bhutia YD, Ramachandran S et al. Deletion of the amino acid transporter Slc6a14 suppresses tumor growth in spontaneous mouse models of breast cancer. Biochem. J. 2015 May 13 [PMID: 25967569] (IHC-P, Mouse)
• Park H, Lee DS, Yim MJ. 3,3'-Diindolylmethane inhibits VEGF expression through the HIF-1alpha and NF-kB pathways in human retinal pigment epithelial cells under chemical hypoxic conditions. Int. J. Mol. Med. 2015 May 05 [PMID: 25955241] (WB, Human)
• Takata S, Masuda T, Nakamura S et al. The effect of triamcinolone acetonide on laser-induced choroidal neovascularization in mice using a hypoxia visualization bio-imaging probe. Sci Rep. 2015 May 01 [PMID: 25927172] (WB, Mouse, Human)
• Liu X, Chen Z, Xu C et al. Repression of hypoxia-inducible factor alpha signaling by Set7-mediated methylation Nucleic Acids Res. 2015 Apr 20 [PMID: 25897119]
• Brandes F, Schmidt K, Wagner C, et al. Targeting cMET with INC280 impairs tumour growth and improves efficacy of gemcitabine in a pancreatic cancer model. BMC Cancer 2015 Feb 19 [PMID: 25884642] (WB, Human)
• Shao J, Zhang Y, Yang T et al. HIF-1alpha disturbs osteoblasts and osteoclasts coupling in bone remodeling by up-regulating OPG expression In Vitro Cell. Dev. Biol. Anim. 2015 Apr 10 [PMID: 25859979] (WB, ChIP, Mouse)
• Chan N, He S, Spee CK et al. Attenuation of choroidal neovascularization by histone deacetylase inhibitor. PLoS One 2015 [PMID: 25807249] (WB, Human)
• Lehmann H, Vicari D, Wild PJ, Frew IJ Combined Deletion of Vhl and Kif3a Accelerates Renal Cyst Formation. J Am Soc Nephrol 2015 Nov [PMID: 25788526]
• Wang F, Zhang G, Xing T et al. Renalase contributes to the renal protection of delayed ischaemic preconditioning via the regulation of hypoxia-inducible factor-1a. J. Cell. Mol. Med. 2015 Mar 17 [PMID: 25781495] (WB, Human)
• Fillmore N, Huqi A, Jaswal JS et al. Effect of Fatty acids on human bone marrow mesenchymal stem cell energy metabolism and survival. PLoS ONE. 2015 Mar 15 [PMID: 25768019] (WB, Human)
• Srivastava S, Thakkar B, Yeoh KG et al. Expression of proteins associated with hypoxia and Wnt pathway activation is of prognostic significance in hepatocellular carcinoma Virchows Arch. 2015 Mar 06 [PMID: 25742908] (IHC-P, Human)
• Paeng SH, Jung WK, Park WS et al. Caffeic acid phenethyl ester reduces the secretion of vascular endothelial growth factor through the inhibition of the ROS, PI3K and HIF-1 alpha signaling pathways in human retinal pigment epithelial cells under hypoxic conditions Int. J. Mol. Med. 2015 May 01 [PMID: 25738890] (WB, Human)
• Rees ML, Subramaniam J, Li Y et al. A PKM2 signature in the failing heart. Biochem Biophys Res Commun 2015 Apr 10 [PMID: 25735978]
• Hasan AU, Ohmori K, Konishi K et al. Eicosapentaenoic acid upregulates VEGF-A through both GPR120 and PPARgamma mediated pathways in 3T3-L1 adipocytes Mol. Cell. Endocrinol. 2015 Feb 16 [PMID: 25697344] (WB, Mouse)
• Li R, Wang Y, Yang Z et al. Hypoxia-inducible factor-1alpha regulates the expression of L-type voltage-dependent Ca(2+) channels in PC12 cells under hypoxia Cell Stress Chaperones. 2015 Feb 04 [PMID: 25648081] (WB, Rat)
• Colbert LE, Fisher SB, Balci S et al. High nuclear hypoxia-inducible factor 1 alpha expression is a predictor of distant recurrence in patients with resected pancreatic adenocarcinoma. Int J Radiat Oncol Biol Phys 2015 Mar 1 [PMID: 25596110] (Human)
• Wang WK, Chen MC, Leong HF et al. Connexin 43 suppresses tumor angiogenesis by down-regulation of vascular endothelial growth factor via hypoxic-induced factor-1a Int J Mol Sci. 2014 Dec 26 [PMID: 25548899] (WB, ELISA, Mouse)
• Hung YH, Chang SH, Huang CT et al. Inhibitor of Differentiation-1 and Hypoxia-Inducible Factor-1 Mediate Sonic Hedgehog Induction by Amyloid Beta-Peptide in Rat Cortical Neurons. Mol. Neurobiol. 2014 Dec 15 [PMID: 25502463] (ChIP, Rat)
• Jeong S, Park H, Hong S et al. Lipophilic modification enhances anti-colitic properties of rosmarinic acid by potentiating its HIF-prolyl hydroxylases inhibitory activity. Eur. J. Pharmacol. 2014 Dec 04 [PMID: 25483211] (WB, Human)
  
  Details:
  HIF1 alpha antibody used for WB in experiments involving human colon carcinoma HCT116 and HT29 cells, human renal cancer UMRC2 cells and UMRC2/VHL cells (stably transfected with VHL)
• Rutz S, Kayagaki N, Phung QT et al. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature. 2014 Dec 03 [PMID: 25470037] (WB, Mouse)
  
  Details:
  HIF1 alpha antibody used for WB on lysates of Duba+/+ and Duba-/- mouse CD4+ T(H)17 cells stimulated with anti-CD3 and anti-CD28 antibodies in the presence of TGF-b and IL-6 (Figure 7).
• Samanta D, Gilkes D, Chaturvedi P, Xiang L, Semenza G. Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells. Proc Natl Acad Sci U S A. 2014 Dec 1 [PMID: 25453096] (WB, Human)
• Gillespie DL, Aguirre MT, Ravichandran S et al. RNA interference targeting hypoxia-inducible factor 1alpha via a novel multifunctional surfactant attenuates glioma growth in an intracranial mouse model. J. Neurosurg. 2014 Nov 25 [PMID: 25423275] (IHC-P, Human)
  
  Details:
  HIF1 alpha antibody used for IHC-P on sections of orthotopic mouse model with U87-LucNeo cells subjected to RNAi mediated knock down HIF-1 alpha in vivo
• Wu X, He L, Chen F et al. Impaired autophagy contributes to adverse cardiac remodeling in acute myocardial infarction. PLoS OnE. 2014 Nov 21 [PMID: 25409294] (WB, Mouse)
• Sato Y, Miyauchi Y, Yoshida S et al. The Vitamin D Analogue ED71 but not 1,25(OH)2D3 Targets HIF1Alpha Protein in Osteoclasts PLoS OnE et al. 2014 Nov 07 [PMID: 25375896] (WB, Mouse)
• Rahman Su, Lee Ms, Baek Jh et al. The Prolyl Hydroxylase Inhibitor Dimethyloxalylglycine Enhances Dentin Sialophoshoprotein Expression through VEGF-Induced Runx2 Stabilization PLoS OnE et al. 2014 Nov 05 [PMID: 25369078] (WB, Rat)
  
  Details:
  HIF1 alpha antibody used for WB on the whole cell lysates of MDPC-23 cells (a rat odontoblast-like cell line) incubated for 24 h in the presence or absence of DMOG 0-1 mM concentration (Figure 1B).
• Assar Me, Sanchez-Puelles Jm, Royo I et al. FM19G11 reverses endothelial dysfunction in rat and human arteries through stimulation of the PI3K/Akt/enOS pathway, independently of mTOR/HIF-1Alpha activation Br. J. Pharmacol. 2014 Nov 03 [PMID: 25363469] (WB, Rat)
  
  Details:
  HIF-1 alpha antibody used for WB on lysates of ex vivo FM19G11 treated aortic and mesenteric arteries from control and insulin-resistant rats (Figure 6).
• Funk K, Scheerer N, Verhaegh R et al. Severe blunt muscle trauma in rats: only marginal hypoxia in the injured area PLoS OnE et al. 2014 Nov 01 [PMID: 25360779] (IHC-P, Rat)
  
  Details:
  HIF1 alpha antibody used for IHC-P on right Musculus gastrocnemius tissue section of male Wistar rats subjected to severe blunt muscle trauma with standardized weight-drop device - paraformaldehyde-fixed-paraffin-embedded sections, primary antibody dilution of 1:10000, catalyzed signal amplification system from DAKO, staining development with DAB-Hematoxylin (Figure 5).
• Marhold M, Tomasich E, El-Gazzar A et al. HIF-1alpha Regulates mTOR Signaling and Viability of Prostate Cancer Stem Cells. Mol. Cancer Res. 2014 Oct 27 [PMID: 25349289] (WB, IP, Human, Mouse)
• He YL, Li MM, Wu LY et al. Enhanced Hypoxia-Inducible Factor (HIF)-1 alpha Stability Induced by 5-Hydroxymethyl-2-Furfural (5-HMF) Contributes to Protection against Hypoxia Mol. Med. 2015 Mar 27 [PMID: 25333920] (WB, ICC/IF, Mouse, Rat)
• Yoshikawa N, Shimizu N, Ojima H et al. Down-regulation of hypoxia-inducible factor-1 alpha and vascular endothelial growth factor by HEXIM1 attenuates myocardial angiogenesis in hypoxic mice. Biochem. Biophys. Res. Commun. 2014 Oct 06 [PMID: 25301555] (WB, Human)
• Kasper LH, Qu C, Obenauer JC et al. Genome-wide and single-cell analyses reveal a context dependent relationship between CBP recruitment and gene expression. Nucleic Acids Res. 2014 Sep 23 [PMID: 25249627] (ChIP, Mouse)
• Bordji K, Grandval A, Cuhna-Alves L et al. Hypoxia-inducible factor-2a (HIF-2a), but not HIF-1a, is essential for hypoxic induction of class III b-tubulin expression in human glioblastoma cells. FEBS J. 2014 Sep 22 [PMID: 25244496] (WB, GS, Human)
  
  Details:
  HIF-1 alpha antibody used for WB and EMSA/Gel Super Shift Assay in experiments involving human glioblastoma cell lines - U87MG (p53 wild-type, PTEN mutant), U251MG (p53 mutant, PTEN mutant) and GL15 (obtained from a frontal lobe tumor biopsy of a 59-year-old man with GBM).
• Shinohara M, Kibi M, Riley IR et al. Cell-Cell Interactions and Bronchoconstrictor Eicosanoid Reduction With Inhaled Carbon Monoxide and Resolvin D1. Am. J. Physiol. Lung Cell Mol. Physiol. 2014 Sep 12 [PMID: 25217660] (ICC/IF, Human)
• Lee HS, Kim KS, Lim HS et al. Priming Wharton's jelly-derived mesenchymal stromal/stem cells with ROCK inhibitor improves recovery in an intracerebral hemorrhage model. J Cell. Biochem. 2014 Sep 04 [PMID: 25185536]
• Fujikuni N, Yamamoto H, Tanabe K et al. Hypoxia-mediated CD24 expression is correlated with gastric cancer aggressiveness by promoting cell migration and invasion. Cancer Sci 2014 Nov [PMID: 25174257] (WB)
• Queisser MA, Dada LA, Deiss-Yehiely N. HOIL-1L Functions as the PKC-zeta Ubiquitin Ligase to Promote Lung Tumor Growth. Am. J. Respir. Crit. Care Med. 2014 Aug 13 [PMID: 25118570]
• Huang T, Huang W, Zhang Z et al. Hypoxia-inducible factor-1a upregulation in microglia following hypoxia protects against ischemia-induced cerebral infarction. Neuroreport. 2014 Oct 01 [PMID: 25089804]
• Gu J, Fan Y, Liu X et al. SENP1 protects against myocardial ischaemia/reperfusion injury via a HIF1a-dependent pathway. Cardiovasc. Res. 2014 Jul 31 [PMID: 25082844] (WB, IP, Rat)
• Yang Xi, Zhu Hongcheng, Ge Yangyang et al. Melittin enhances radiosensitivity of hypoxic head and neck squamous cell carcinoma by suppressing HIF-1alpha. Tumour Biol. 2014 Jul 23 [PMID: 25053591]
• Zhou W, Marinescu M, Veltkamp R. Only Very Early Oxygen Therapy Attenuates Posthemorrhagic Edema Formation and Blood-Brain Barrier Disruption in Murine Intracerebral Hemorrhage. Neurocrit Care 2014 Jul 15 [PMID: 25022804] (WB, Mouse)
  
  Details:
  HIF-1 alpha antibody used for WB (1:500 dilution) on lysates of serial coronal cryosections of hemorrhagic and nonhemorrhagic hemispheres from C57BL/6 male mice 3d after inducing Intracerebral Hemorrhage/ ICH (Fig 6B).
• Abu-Remaileh M, Aqeilan RI. Tumor suppressor WWOX regulates glucose metabolism via HIF1alpha modulation. Cell Death Differ 2014 Nov [PMID: 25012504] (WB)
• Kalamida D, Karagounis IV, Giatromanolaki A, Koukourakis MI. Important role of autophagy in endothelial cell response to ionizing radiation. PLoS ONE 2014 Jul 11 [PMID: 25010689] (WB, Human)
  
  Details:
  HIF-1 alpha antibody used for WB on lysates of HUVEC/human umbilical vein endothelial cells after exposure to 2 Gy of IR with or without 2 day pre-incubation with SMER28/Small-Molecule Enhancer of Rapamycin-28 (an mTOR-independent inducer of autophagy). Primary antibody was used at 1:500 dilution and the blot images are not shown in the article.
• Privat M, Radosevic-Robin N, Aubel C et al. BRCA1 Induces Major Energetic Metabolism Reprogramming in Breast Cancer Cells. PLoS ONE 2014 Jul 11 [PMID: 25010005] (WB, Human)
  
  Details:
  HIF-1 alpha antibody used for WB on hypoxia mimetic CoCl2 treated /not treated SUM1315-BRCA1 and SUM1315-LXSN cell lines which were obtained by stable transfection of SUM1315 human breast cancer cells with an empty LXSN plasmid, or a BRCA1-encoding LXSN plasmid (WB image shown in Figure S1).
• Huang Miaoling, Chen Qing, Xiao Jianpeng et al. Overexpression of hypoxia-inducible factor-1alpha is a predictor of poor prognosis in cervical cancer: a clinicopathologic study and a meta-analysis. Int J Gynecol Cancer. 2014 Jul 01 [PMID: 24978711] (Human)
• Yang MY, Chiao MT, Lee HT et al. An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior J. Biomed. Mater. Res. Part B Appl. Biomater. 2014 Jun 26 [PMID: 24966152] (IHC, Human)
  
  Details:
  HIF-1 alpha antibody used for IHC -DAB of xenograft tissue of human glioblastoma stem cells/GSCs grown in gelatin foam. Figure 5 f - HIF1 alpha used as an intrinsic marker of tumor hypoxia.
• Wu JB, Shao C, Li X et al. Near-infrared fluorescence imaging of cancer mediated by tumor hypoxia and HIF1alpha/OATPs signaling axis. Biomaterials. 2014 Jun 20 [PMID: 24957295] (IHC-P, Human)
  
  Details:
  Archival formalin-fixed paraffin-embedded/FFPE human prostate cancer/PCa specimens were processed for IHC staining with HIF1 alpha antibody (clone H1alpha67, 1:30 dilution).
• Narise Kosuke, Okuda Kensuke, Enomoto Yukihiro et al. Optimization of biguanide derivatives as selective antitumor agents blocking adaptive stress responses in the tumor microenvironment. Drug Des Devel Ther. 2014 Jun 06 [PMID: 24944508] (WB)
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  Details:
  HIF 1 alpha antibody used for FFPE liver sections from male Sprague-Dawley rats treated or not with PCB 126 (AhR agonist ) - HIER using citrate buffer pH 6.0, Peroxidase quenching with 3% H2O2 for 8 min, blocking with 5% horse serum, 1:50 primary - 2 h incubation at RT, detected via Mouse Envision HRP System Reagent -DAB method. Fig 1 - PCB 126 treated livers had mainly cytoplasmic/decreased nuclear staining compared to control rats.
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• Xu W, Kwon JH, Moon YH et al. Influence of preoperative transcatheter arterial chemoembolization on gene expression in the HIF-1alpha pathway in patients with hepatocellular carcinoma. J. Cancer Res. Clin. Oncol. 2014 May 23 [PMID: 24853275] (IHC-P, Human)
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• Zheng J, Song F, Lu SL, Wang XQ. Dynamic Hypoxia in Scar Tissue During Human Hypertrophic Scar Progression. Dermatol Surg 2014 Mar 31 [PMID: 24684437] (IHC-P, Human)
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• Pan WL, Wong JH, Fang EF et al. Preferential cytotoxicity of the type I ribosome inactivating protein alpha-momorcharin on human nasopharyngeal carcinoma cells under normoxia and hypoxia. Biochem. Pharmacol. 3/14/2014 [PMID: 24637239] (WB, Human)
• Zhao L, Yang YF, Gao YB et al. Upregulation of HIF-1a Via Activation of ERK and PI3K Pathway Mediated Protective Response to Microwave-Induced Mitochondrial Injury in Neuron-Like Cells. Mol. Neurobiol. 3/14/2014 [PMID: 24627260] (WB, Rat)
• Gauter-Fleckenstein Benjamin, Reboucas Julio S, Fleckenstein Katharina et al. Robust rat pulmonary radioprotection by a lipophilic Mn N-alkylpyridylporphyrin, MnTnHex-2-PyP(5+). Redox Biol. 2014 Jan 09 [PMID: 24624330] (IHC, Rat)
• Shan F, Li J, Huang QY. HIF-1 Alpha-Induced Up-Regulation of miR-9 Contributes to Phenotypic Modulation in Pulmonary Artery Smooth Muscle Cells During Hypoxia. J. Cell. Physiol. 2/25/2014 [PMID: 24615545] (WB, Rat)
• Imamura Y, Tomita S, Imanishi M et al. HIF-2a/ARNT complex regulates hair development via induction of p21Waf1/Cip1 and p27Kip1. FASEB J. 3/5/2014 [PMID: 24599965] (WB, Mouse)
• Huang YH, Lin MH, Wang PC et al. Hypoxia inducible factor 2a/insulin-like growth factor receptor signal loop supports the proliferation and Oct-4 maintenance of mouse germline stem cells. Mol. Hum. Reprod. 3/23/2014 [PMID: 24598112] (WB, Mouse)
• Li Y, Chen J, Zeng T et al. Expression of HIF-1a and MDR1/P-glycoprotein in refractory mesial temporal lobe epilepsy patients and pharmacoresistant temporal lobe epilepsy rat model kindled by coriaria lactone. Neurol. Sci. 3/4/2014 [PMID: 24590840] (WB, Rat)
• Zhao W, Chang C, Cui Y et al. Steroid Receptor Coactivator-3 Regulates Glucose Metabolism in Bladder Cancer Cells through Coactivation of Hypoxia Inducible-Factor 1 alpha. J. Biol. Chem. 2014 Mar 3 [PMID: 24584933] (ChIP, Human)
• Lall R, Ganapathy S, Yang M et al. Low-dose radiation exposure induces a HIF-1-mediated adaptive and protective metabolic response. Cell Death Differ. 2014 Feb 28 [PMID: 24583639] (ICC/IF, Human)
• He M, Lu Y, Xu S et al. MiRNA-210 modulates a nickel-induced cellular energy metabolism shift by repressing the iron-sulfur cluster assembly proteins ISCU1/2 in Neuro-2a cells. Cell Death Dis 2/28/2014 [PMID: 24577088] (WB, Mouse)
• Zhang Xiaonan, Fryknas Marten, Hernlund Emma et al. Induction of mitochondrial dysfunction as a strategy for targeting tumour cells in metabolically compromised microenvironments. Nat Commun. 2014 [PMID: 24548894]
• Mateo J, Izquierdo-Garcia D, Badimon JJ, Fuster V. Noninvasive Assessment of Hypoxia in Rabbit Advanced Atherosclerosis Using 18F-fluoromisonidazole PET Imaging. Circ Cardiovasc Imaging 2014 Feb 10 [PMID: 24508668] (IHC-P, Human)
• Bohuslavova Romana, Kolar Frantisek, Sedmera David et al. Partial deficiency of HIF-1alpha stimulates pathological cardiac changes in streptozotocin-induced diabetic mice. BMC Endocr Disord. 2014 Feb 06 [PMID: 24502509] (WB, Mouse)
• White NM, Masui O, Newsted D et al. Galectin-1 has potential prognostic significance and is implicated in clear cell renal cell carcinoma progression through the HIF/mTOR signaling axis. Br. J. Cancer 2014 Mar 5 [PMID: 24496460] (WB, Human)
• Weisel FC, Kloepping C, Pichl A et al. Impact of S-Adenosylmethionine Decarboxylase 1 in Pulmonary Vascular Remodeling. Circulation 2014 Jan 28 [PMID: 24470481] (ChIP, Mouse, Human)
• Wang Enfeng, Zhang Chunyang, Polavaram Navatha et al. The role of factor inhibiting HIF (FIH-1) in inhibiting HIF-1 transcriptional activity in glioblastoma multiforme. PloS One 2014 [PMID: 24465898] (IP, WB, Human)
• Chen Z, Liu X, Mei Z, Xiao W. EAF2 Suppresses Hypoxia-Induced Factor 1 alpha Transcriptional Activity by Disrupting Its Interaction with Coactivator CBP/p300. Mol. Cell. Biol. 2014 Mar 1 [PMID: 24421387] (WB, Human)
• Pantel A, Teixeira A, Haddad E et al. Direct type I IFN but not MDA5/TLR3 activation of dendritic cells is required for maturation and metabolic shift to glycolysis after poly IC stimulation. PLoS Biol. 2014 Jan 1 [PMID: 24409099] (FLOW, Mouse)
• Garofalo C, Capristo M, Manara MC et al. Metformin as an Adjuvant Drug against Pediatric Sarcomas: Hypoxia Limits Therapeutic Effects of the Drug. PLoS One 2013 Dec 31 [PMID: 24391834] (IHC-P, Human)
• Ganapathy S, Xiao S, Yang M et al. A low-dose arsenic induced p53-mediated metabolic mechanism of radiotherapy protection. J Biol Chem 2014 Jan 3 [PMID: 24391088] (ICC/IF, Human)
• Kind KL, Tam KK, Banwell KM et al. Oxygen-regulated gene expression in murine cumulus cells. Reprod Fertil Dev 2014 Jan 6 [PMID: 24388334] (WB, Mouse)
• Engelhardt S, Al-Ahmad AJ, Gassmann M, Ogunshola OO. Hypoxia Selectively Disrupts Brain Microvascular Endothelial Tight Junction Complexes Through A Hypoxia-Inducible Factor-1 (HIF-1) Dependent Mechanism. J Cell Physiol 2013 Dec 25 [PMID: 24375098] (WB, Rat)
• Miyazaki S, Kikuchi H, Iino I et al. Anti-VEGF antibody therapy induces tumor hypoxia and stanniocalcin 2 expression and potentiates growth of human colon cancer xenografts. Int J Cancer 2013 Dec 20 [PMID: 24375080] (IHC-P, Human)
• Ji X, Wang H, Zhu J et al. Knockdown of Nrf2 suppresses glioblastoma angiogenesis by inhibiting hypoxia-induced activation of HIF-1alpha. Int J Cancer 2013 Dec 26 [PMID: 24374745] (WB, Human)
• Kawakami T, Yoshimi M, Kadota Y et al. Prolonged endoplasmic reticulum stress alters placental morphology and causes low birth weight. Toxicol Appl Pharmacol 2013 Dec 23 [PMID: 24370435] (IHC-P, Mouse)
• Ouyang W, Torigoe C, Fang H et al. Anthrax lethal toxin inhibits translation of hypoxia inducible factor 1alpha and causes decreased tolerance to hypoxic stress. J Biol Chem 2013 Dec 23 [PMID: 24366872] (WB, Mouse)
• Popescu CR, Sutherland MR, Cloutier A et al. Hyperoxia Exposure Impairs Nephrogenesis in the Neonatal Rat: Role of HIF-1alpha. PLoS One 2013 Dec 17 [PMID: 24358181] (IHC-P, Rat)
• An X, Xu G, Yang L et al. Expression of hypoxia-inducible factor-1alpha, vascular endothelial growth factor and prolyl hydroxylase domain protein 2 in cutaneous squamous cell carcinoma and precursor lesions and their relationship with histological stages and clinical features. J Dermatol 2013 Dec 20 [PMID: 24354513] (IHC-P, Human)
• Park HJ, Kim MK, Kim SR. Hypoxia Regulates the Expression of the Neuromedin B Receptor through a Mechanism Dependent on Hypoxia-Inducible Factor-1A. PLoS One 2013 Dec 9 [PMID: 24349381] (WB, ICC/IF, IHC-P, ChIP, Human)
• Fujimura A, Michiue H, Cheng Y et al. Cyclin g2 promotes hypoxia-driven local invasion of glioblastoma by orchestrating cytoskeletal dynamics. Neoplasia 2013 Nov [PMID: 24339739] (WB, ICC/IF, Human)
• Fernandes BF, Coates J, Odashiro AN et al. Hypoxia-Inducible Factor-1alpha and its Role in the Proliferation of Retinoblastoma Cells. Pathol Oncol Res 2013 Dec 14 [PMID: 24338218] (WB, Human)
• Chen TM, Shih YH, Tseng JT et al. Overexpression of FGF9 in colon cancer cells is mediated by hypoxia-induced translational activation. Nucleic Acids Res 2013 Dec 10 [PMID: 24334956] (IHC, Human)
• Takano N, Peng YJ, Kumar GK et al. Hypoxia-inducible factors regulate human and rat cystathionine B-synthase gene expression. Biochem J 2013 Dec 12 [PMID: 24328859] (WB, ChIP, Human, Rat)
• Zhang C, Yang X, Zhang Q et al. Berberine radiosensitizes human nasopharyngeal carcinoma by suppressing hypoxia-inducible factor-1alpha expression. Acta Otolaryngol 2014 Feb [PMID: 24325635] (WB, Human)
• Chen Z, He X, Xia W et al. Prognostic Value and Clinicopathological Differences of HIFs in Colorectal Cancer: Evidence from Meta-Analysis. PLoS One 2013 Dec 6 [PMID: 24324596] (IHC, Human)
• Jensen RL, Mumert ML, Gillespie DL et al. Preoperative dynamic contrast-enhanced MRI correlates with molecular markers of hypoxia and vascularity in specific areas of intratumoral microenvironment and is predictive of patient outcome. Neuro Oncol 2013 Dec 4 [PMID: 24305704] (IHC-P, Human)
• Watson CJ, Collier P, Tea I et al. Hypoxia-induced epigenetic modifications are associated with cardiac tissue fibrosis and the development of a myofibroblast-like phenotype. Hum Mol Genet 2013 Dec 16 [PMID: 24301681] (WB, Human)
• Ma Z, Moruzzi N, Catrina SB et al. Hyperoxia inhibits glucose-induced insulin secretion and mitochondrial metabolism in rat pancreatic islets. Biochem Biophys Res Commun. 2013 Dec 2 [PMID: 24299957] (WB, Rat)
• Przybyla Beata D, Shafirstein Gal, Vishal Sagar J et al. Molecular changes in bone marrow, tumor and serum after conductive ablation of murine 4T1 breast carcinoma. Int J Oncol. 2014 Feb 01 [PMID: 24270800] (WB)
• Bertozzi D, Marinello J, Manzo SG et al. The natural inhibitor of DNA topoisomerase I, camptothecin, modulates HIF-1 alpha activity by changing miR expression patterns in human cancer cells. Mol. Cancer Ther. 2014 Jan 1 [PMID: 24252850] (WB, Human)
• Liu X, Tu Y, Zhang L et al. Prolyl Hydroxylase Inhibitors Protect from the Bone Loss in Ovariectomy Rats by Increasing Bone Vascularity. Cell Biochem Biophys. 2013 Nov 16 [PMID: 24242187] (WB, Rat)
• Koide N, Odkhuu E, Naiki Y et al. Augmentation of LPS-induced vascular endothelial cell growth factor production in macrophages by transforming growth factor-beta1. Innate Immun. 2013 Nov 13 [PMID: 24225655] (WB, Mouse)
• Zhang Y, Marsboom G, Toth PT, Rehman J. Mitochondrial respiration regulates adipogenic differentiation of Human mesenchymal stem cells. PLoS One. 2013 Oct 18 [PMID: 24204740] (ICC/IF, Human)
• Park EY, Lee CH, Lee EK et al. HNF4alpha contributes to glucose formation in aged rat hepatocytes. Exp Gerontol 2013 Dec [PMID: 24177414] (WB, Rat)
• Huang SM, Chen TS, Chiu CM et al. GDNF increases cell motility in human colon cancer through VEGF-VEGFR1 interaction. Endocr. Relat. Cancer 2014 Feb 1 [PMID: 24165321] (WB, Human)
• Madej JA, Madej JP, Dziegiel P et al. Expression of hypoxia-inducible factor-1alpha and vascular density in mammary adenomas and adenocarcinomas in bitches. Acta Vet Scand. 2013 Oct 24 [PMID: 24153191] (IHC-P, Canine)
• Cheng YL, Park JS, Manzanero S et al. Evidence that collaboration between HIF-1 alpha and Notch-1 promotes neuronal cell death in ischemic stroke. Neurobiol. Dis. 2014 Feb 1 [PMID: 24141018] (WB, ICC/IF, Mouse)
• Eckle T, Brodsky K, Bonney M et al. HIF1A Reduces Acute Lung Injury by Optimizing Carbohydrate Metabolism in the Alveolar Epithelium. PLoS Biol. 2013 Sep [PMID: 24086109] (WB, IHC-P, Human, Mouse)
• Zhang L, Xu J, Sun N et al. The Presence of Adenosine A2a Receptor in Thyrocytes and Its Involvement in Graves' IgG-Induced VEGF Expression. Endocrinology. 2013 Sep 30 [PMID: 24080368] (WB, IHC-P, ICC/IF, Mouse, Human)
• Hu P, Chung LW, Berel D et al. Convergent RANK- and c-Met-Mediated Signaling Components Predict Survival of Patients with Prostate Cancer: An Interracial Comparative Study. PLoS One. 2013 Sep 16 [PMID: 24066029] (Human)
• Wall AM, Corcoran AE, O'Halloran KD, O'Connor JJ. Effects of prolyl-hydroxylase inhibition and chronic intermittent hypoxia on synaptic transmission and plasticity in the rat CA1 and dentate gyrus. Neurobiol Dis. 2013 Sep 17 [PMID: 24055213] (WB, Rat)
• Gallaher AM, Das S, Xiao Z et al. Proteomic Screening of Human Targets of Viral microRNAs Reveals Functions Associated with Immune Evasion and Angiogenesis. PLoS Pathog. 2013 Sep [PMID: 24039573] (WB, Human)
• Cowburn AS, Alexander LE, Southwood M et al. Epidermal Deletion of HIF-2alpha Stimulates Wound Closure. J Invest Dermatol. 2013 Sep 13 [PMID: 24037341] (IHC-P, Mouse)
• Minoia C, Quero C, Asselti M et al. Changes in angiogenesis and Hypoxia-Inducible Factor-1alpha protein expression in relapsed/refractory indolent non-Hodgkin Lymphomas. Br J Haematol. 2013 Sep 13 [PMID: 24033233] (IHC, Human)
• Yang X, Yang B, Cai J et al. Berberine enhances radiosensitivity of esophageal squamous cancer by targeting HIF-1alpha in vitro and in vivo. Cancer Biol Ther 2013 Nov [PMID: 24025355] (Mouse)
• Miyauchi Y, Sato Y, Kobayashi T et al. HIF1alpha is required for osteoclast activation by estrogen deficiency in postmenopausal osteoporosis. Proc Natl Acad Sci U S A. 2013 Oct 8 [PMID: 24023068] (WB, IHC-P, ICC/IF, Mouse)
• Tan JT, Prosser HC, Vanags LZ et al. High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1alpha. FASEB J. 2013 Sep 10 [PMID: 24022405] (WB, Human)
• Ding G, Huang G, Liu HD et al. MiR-199a suppresses the hypoxia-induced proliferation of non-small cell lung cancer cells through targeting HIF1alpha. Mol Cell Biochem. 2013 Sep 11 [PMID: 24022342] (WB, IHC, Human)
• Wang Wen-Sheng, Liang Hong-Yin, Cai Yu-Jiao, Yang Hua. DMOG ameliorates IFN-gamma-induced intestinal barrier dysfunction by suppressing PHD2-dependent HIF-1alpha degradation. J Interferon Cytokine Res. 2014 Jan 01 [PMID: 24010824] (Human)
• Lin WP, Wang XJ, Wang CR et al. Polymorphism in the Hypoxia-Inducible Factor 1alpha Gene May Confer Susceptibility to LDD in Chinese Cohort. PLoS One. 2013 Aug 26 [PMID: 23991178] (WB, Human)
• Pawlus MR, Wang L, Murakami A. STAT3 or USF2 Contributes to HIF Target Gene Specificity. PLoS One. 2013 Aug 21 [PMID: 23991099] (ChIP, Human)
• Cao Y, Eble JM, Moon E et al. Tumor Cells Upregulate Normoxic HIF-1alpha in Response to Doxorubicin. Cancer Res. 2013 Aug 19 [PMID: 23959856] (WB, Human, Mouse)
• Wang SC, Yu CF, Hong JH et al. Radiation Therapy-Induced Tumor Invasiveness Is Associated with SDF-1-Regulated Macrophage Mobilization and Vasculogenesis. PLoS One. 2013 Aug 5 [PMID: 23940516] (WB, Mouse)
• Shin JM, Jeong YJ, Cho HJ et al. Melittin Suppresses HIF-1alpha/VEGF Expression through Inhibition of ERK and mTOR/p70S6K Pathway in Human Cervical Carcinoma Cells. PLoS One 2013 Jul 23 [PMID: 23936001] (ChIP, Human)
• Ma Z, Moruzzi N, Catrina SB et al. Preconditioning with Associated Blocking of Ca2+ Inflow Alleviates Hypoxia-Induced Damage to Pancreatic beta-Cells. PLoS One 2013 Jul 25 [PMID: 23935835] (WB, Rat)
• Jung JH, Im S, Jung ES, Kang CS. Clinicopathological Implications of the Expression of Hypoxia-related Proteins in Gastric Cancer. Int J Med Sci 2013 Jul 31 [PMID: 23935399] (IHC-P, Human)
• Takibuchi G, Imanishi H, Morimoto M et al. Polymorphic mutations in mouse mitochondrial DNA regulate a tumor phenotype. Mitochondrion. 2013 Aug 6 [PMID: 23932994] (WB, Mouse)
• Zaccagnini G, Maimone B, Di Stefano V et al. Hypoxia-induced miR-210 modulates tissue response to acute peripheral ischemia. Antioxid Redox Signal. 2013 Aug 10 [PMID: 23931770]
• Al-Greene NT, Means AL, Lu P et al. Four Jointed Box 1 Promotes Angiogenesis and Is Associated with Poor Patient Survival in Colorectal Carcinoma. PLoS One 2013 Jul 29 [PMID: 23922772] (WB, Human)
• Belloli S, Brioschi A, Politi LS et al. Characterization of biological features of a rat F98 GBM model: A PET-MRI study with [(18)F]FAZA and [(18)F]FDG. Nucl Med Biol 2013 Aug [PMID: 23915802] (IHC-P, Rat)
• Sun YY, Lin SH, Lin HC et al. Cell Type-Specific Dependency on the PI3K/Akt Signaling Pathway for the Endogenous Epo and VEGF Induction by Baicalein in Neurons versus Astrocytes. PLoS One 2013 Jul 19 [PMID: 23904909] (ChIP, Rat)
• Park YK, Park B, Lee SY et al. Hypoxia-Inducible Factor-2alpha-Dependent Hypoxic Induction of Wnt10b Expression in Adipogenic Cells. J Biol Chem 2013 Jul 30 [PMID: 23900840] (WB, Mouse)
• Xu H, Li B, Yu W et al. Correlation between 18F-FDG uptake and the expression of glucose transporter-1 and hypoxia-inducible factor-1alpha in transplanted VX2 tumors. Nucl Med Commun 2013 Jul 24 [PMID: 23887273] (IHC-P, Rabbit)
• Fujimoto TN, Colbert LE, Molkentine JM, Baseler L. EGLN Inhibition Reduces Gastrointestinal Radiation Toxicity and Improves Survival in a Murine Model of Locally Advanced Pancreatic Cancer bioRxiv 2018 Jul 20 (WB, Mouse)
• You Q, Holt M, Yin H et al. Role of hepatic resident and infiltrating macrophages in liver repair after acute injury. Biochem Pharmacol 2013 Jul 19 [PMID: 23876342] (WB, Mouse)
• Allon I, Kaplan I, Allon D et al. HIF-1alpha, VEGF, and EGFR: contributing factors in the pathogenesis of necrotizing sialometaplasia. Oral Dis 2013 Jun 25 [PMID: 23837804] (IHC-P, Human)
• Wang W, McKinnie SM, Patel VB et al. Loss of Apelin Exacerbates Myocardial Infarction Adverse Remodeling and Ischemia-reperfusion Injury: Therapeutic Potential of Synthetic Apelin Analogues. J Am Heart Assoc 2013 Jul 1 [PMID: 23817469] (IHC-P, ICC/IF, WB, Mouse)
• Xie L, Collins JF. Transcription Factors Sp1 and Hif2alpha Mediate Induction of the Copper-Transporting ATPase (Atp7a) Gene in Intestinal Epithelial Cells During Hypoxia. J Biol Chem 2013 Jun 28 [PMID: 23814049] (WB, Rat)
• Sundelin JP, Lidberg U, Nik AM et al. Hypoxia-induced regulation of the very low density lipoprotein receptor. Biochem Biophys Res Commun 2013 Jun 27 [PMID: 23811271] (ChIP, Mouse)
• Weaver J, Casellini C, Parson H, Vinik A. EXPRESSION OF HIF-1a IN A PANCREATIC DUCTAL ADENOCARCINOMA IN A PATIENT WITH NEWLY DIAGNOSED TYPE 2 DIABETES AACE Clinical Case Reports May 1 2018
• Takikawa T, Masamune A, Hamada S et al. miR-210 regulates the interaction between pancreatic cancer cells and stellate cells. Biochem Biophys Res Commun 2013 Jul 4 [PMID: 23831622] (ICC/IF, Human)
• Wu C, Zhou Y, Chang J, Xiao Y. Delivery of dimethyloxallyl glycine in mesoporous bioactive glass scaffolds to improve angiogenesis and osteogenesis of human bone marrow stromal cells. Acta Biomater 2013 Jun 26 [PMID: 23811216] (WB, Human)
• Kondrashina AV, Papkovsky DB, Dmitriev RI. Measurement of cell respiration and oxygenation in standard multichannel biochips using phosphorescent O2-sensitive probes. Analyst 2013 Jun 27 [PMID: 23803790]
• Sato Y, Yoshizato T, Shiraishi Y et al. Integrated molecular analysis of clear-cell renal cell carcinoma. Nat Genet 2013 Jun 24 [PMID: 23797736] (IHC, Human)
• Shimomura M, Hinoi T, Kuroda S et al. Overexpression of Hypoxia Inducible Factor-1 Alpha is an Independent Risk Factor for Recurrence After Curative Resection of Colorectal Liver Metastases. Ann Surg Oncol 2013 Jun 9 [PMID: 23748663] (IHC-P, Human)
• Zhang H, Tang L, Chen S et al. Effect of advanced glycation end products on the expression of hypoxia-inducible factor-1alpha and vascular endothelial growth factor proteins in RF/6A cells. Exp Ther Med 2013 May [PMID: 23737911] (WB, ICC/IF, Primate)
• Park GC, Lee M, Roh JL, et al. Phospho-Rb (Ser780) as a biomarker in patients with cervical lymph node metastases from an unknown primary tumour: a retrospective cohort study. Clin Otolaryngol 2013 Jun 4 [PMID: 23731755] (IHC-P, Human)
• Coudyzer P, Lemoine P, Jordan BF et al. Hypoxia is not required for human endometrial breakdown or repair in a xenograft model of menstruation. FASEB J 2013 May 31 [PMID: 23729593] (IHC-P, Human)
• Loukovaara S, Koivunen P, Ingles M et al. Elevated protein carbonyl and HIF-1alpha levels in eyes with proliferative diabetic retinopathy. Acta Ophthalmol 2013 May 29 [PMID: 23718695] (WB, Human)
• Hutter R, Speidl WS, Valdiviezo C et al. Macrophages Transmit Potent Proangiogenic Effects of oxLDL In Vitro and In Vivo Involving HIF-1alpha Activation: a Novel Aspect of Angiogenesis in Atherosclerosis. J Cardiovasc Transl Res 2013 May 10 [PMID: 23661177] (IHC-P, Rabbit)
• Janardhanan R, Yang B, Vohra P et al. Simvastatin reduces venous stenosis formation in a murine hemodialysis vascular access model. Kidney Int 2013 May 1 [PMID: 23636169] (IHC-P, Mouse)
• Musyoka JN, Liu MC, Pouniotis DS et al. Siah2-deficient mice show impaired skin wound repair. Wound Repair Regen 2013 May [PMID: 23627548] (IHC-P, Mouse)
• Bohuslavova R, Skvorova L, Sedmera D et al. Increased susceptibility of HIF-1alpha heterozygous-null mice to cardiovascular malformations associated with maternal diabetes. J Mol Cell Cardiol 2013 Apr 22 [PMID: 23619295] (WB, Mouse)
• Albers J, Rajski M, Schonenberger D et al. Combined mutation of Vhl and Trp53 causes renal cysts and tumours in mice. EMBO Mol Med 2013 Apr 22 [PMID: 23606570] (WB, IHC-P, Mouse)
• Bendinelli P, Maroni P, Matteucci E et al. Hypoxia inducible factor-1 is activated by transcriptional co-activator with PDZ-binding motif (TAZ) versus WWdomain-containing oxidoreductase (WWOX) in hypoxic microenvironment of bone metastasis from breast cancer. Eur J Cancer 2013 Apr 5 [PMID: 23566416] (IHC, Human)
• Chou YT, Zhan G, Zhu Y et al. C/EBP homologous binding protein (CHOP) underlies neural injury in sleep apnea model. Sleep 2013 Apr [PMID: 23564995] (Mouse)
• Li C, Xia M, Abais JM et al. Protective role of growth hormone against hyperhomocysteinemia-induced glomerular injury Naunyn Schmiedebergs Arch Pharmacol 2013 Mar 27 [PMID: 23529346] (WB, Mouse)
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  Details:
  Citation using the DyLight 550 version of this antibody.
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• Kato H, Inoue T, Asanoma K et al. Induction of human endometrial cancer cell senescence through modulation of HIF-1alpha activity by EGLN1. Int J Cancer 2006 Mar 1 [PMID: 16161047]
• Hayashi M, Sakata M, Takeda T et al. Up-regulation of c-met protooncogene product expression through hypoxia-inducible factor-1alpha is involved in trophoblast invasion under low-oxygen tension. Endocrinology 2005 Nov [PMID: 16099863] (ChIP, Human)
• Behan A, Doyle S, Farrell M. Adaptive responses to mitochondrial dysfunction in the rho degrees Namalwa cell. Mitochondrion 2005 Jun [PMID: 16050983] (WB, Human)
• Zagzag, D et al. Stromal cell-derived factor-1alpha and CXCR4 expression in hemangioblastoma and clear cell-renal cell carcinoma: von Hippel-Lindau loss-of-function induces expression of a ligand and its receptor. Can. Res. 65: 6178-6188. 2005 [PMID: 16024619] (WB, IHC, Human)
• Maltepe E, Krampitz GW, Okazaki KM et al. Hypoxia-inducible factor-dependent histone deacetylase activity determines stem cell fate in the placenta. Development 2005 Aug [PMID: 15987772] (WB, Mouse)
• Alberghini A, Recalcati S, Tacchini L et al. Loss of the von Hippel Lindau tumor suppressor disrupts iron homeostasis in renal carcinoma cells. J Biol Chem. 280(34):30120-8. 2005 Aug 26. [PMID: 15985433] (WB, Human)
• Kim SJ, Rabbani ZN, Dewhirst MW et al. Expression of HIF-1alpha, CA IX, VEGF, and MMP-9 in surgically resected non-small cell lung cancer. Lung Cancer 2005 Sep [PMID: 15935515] (IHC, Human)
• Wang, B, Zou, Y, Li, H et al. Genistein Inhibited Retinal Neovascularization Expression of Vascular Endothelial Growth Factor Hypoxia Inducible Factor 1 alpha in a Mouse Model of Oxygen-Induced Retinopathy. J. of Ocular Pharmacology Therapeutics.:107-113. Apr. 2005. [PMID: 15857276] (Mouse)
• Li YM, Zhou BP, Deng J et al. A hypoxia-independent hypoxia-inducible factor-1 activation pathway induced by phosphatidylinositol-3 kinase/Akt in HER2 overexpressing cells. Cancer Res 2005 Apr 15 [PMID: 15833858]
• Kazi AA, Jones JM, Koos RD. Chromatin immunoprecipitation analysis of gene expression in the rat uterus in vivo: estrogen-induced recruitment of both estrogen receptor alpha and hypoxia-inducible factor 1 to the vascular endothelial growth factor promoter. Mol Endocrinol 2005 Aug [PMID: 15774498] (WB)
• Matsuyama T, Nakanishi K, Hayashi T et al. Expression of hypoxia-inducible factor-1alpha in esophageal squamous cell carcinoma. Cancer Sci 2005 Mar [PMID: 15771621]
• Irie N, Matsuo T, Nagata I. Protocol of radiotherapy for glioblastoma according to the expression of HIF-1. Brain Tumor Pathol 2004 [PMID: 15696961] (IHC, Human)
• Aminova LR, Chavez JC, Lee J et al. Prosurvival prodeath effects of hypoxia-inducible factor-1alpha stabilization in a murine hippocampal cell line. J Biol Chem. 280(5):3996-4003. 2005 Feb 4. [PMID: 15557337] (WB, Mouse)
• Knies-Bamforth, UE et al. c-Myc interacts with hypoxia to induce angiogenesis in vivo by a vascular endothelial growth factor-dependent mechanism. Can. Res. 64:6563-6570. 2004 [PMID: 15374969] (WB, Rat)
• Tezel G, Wax MB. Hypoxia-inducible factor 1alpha in the glaucomatous retina and optic nerve head. Arch Ophthalmol 2004 Sep [PMID: 15364715] (IHC-P, Human)
• Swinson DE, Jones JL, Cox G et al. Hypoxia-inducible factor-1 alpha in non small cell lung cancer: relation to growth factor, protease and apoptosis pathways. Int J Cancer 2004 Aug 10 [PMID: 15185341] (IP, Human)
• Mansfield KD, Simon MC, Keith B. Hypoxic reduction in cellular glutathione levels requires mitochondrial reactive oxygen species. J Appl Physiol (1985) 2004 Oct [PMID: 15180977] (WB, Human)
• Nishi H, Nakada T, Hokamura M et al. Hypoxia-inducible factor-1 transactivates transforming growth factor-beta3 in trophoblast. Endocrinology 2004 Sep [PMID: 15155569] (WB, Human)
• Tissot van Patot MC, Bendrick-Peart J, Beckey VE et al. Greater vascularity, lowered HIF-1/DNA binding, and elevated GSH as markers of adaptation to in vivo chronic hypoxia. Am J Physiol Lung Cell Mol Physiol 2004 Sep [PMID: 15132953] (WB, Human)
• Reichner JS, Albina JE. Determination of the role of hypoxia-inducible factor 1 in wound healing. Methods Enzymol 2004 [PMID: 15063696] (Rat)
• Pascual O, Roux JC, Soulage C et al. Carotid chemodenervation approach to study oxygen sensing in brain stem catecholaminergic cells. Methods Enzymol 2004 [PMID: 15063691] (IHC, Rat)
• Yatabe N, Kyo S, Maida Y et al. HIF-1-mediated activation of telomerase in cervical cancer cells. Oncogene 2004 Apr 29 [PMID: 15048086] (ChIP, Human)
• Wykoff CC, Sotiriou C, Cockman ME et al. Gene array of VHL mutation and hypoxia shows novel hypoxia-induced genes and that cyclin D1 is a VHL target gene. Br J Cancer 2004 Mar 22 [PMID: 15026807] (Human)
• Erler JT, Cawthorne CJ, Williams KJ et al. Hypoxia-mediated down-regulation of Bid and Bax in tumors occurs via hypoxia-inducible factor 1-dependent and-independent mechanisms and contributes to drug Mol Cell Biol. 2004 Apr [PMID: 15024076] (WB, Mouse, Human)
• Palladino MA, Powell JD, Korah N, Hermo L. Expression and localization of hypoxia-inducible factor-1 subunits in the adult rat epididymis. Biol Reprod 2004 Apr [PMID: 14668208] (WB, Rat)
• Hu CJ, Wang LY, Chodosh LA et al. Differential roles of hypoxia-inducible factor 1alpha (HIF-1alpha) and HIF-2alpha in hypoxic gene regulation. Mol Cell Biol 2003 Dec [PMID: 14645546] (WB)
• Liu MY, Poellinger L, Walker CL. Up-regulation of hypoxia-inducible factor 2alpha in renal cell carcinoma associated with loss of Tsc-2 tumor suppressor gene. Cancer Res 2003 May 15 [PMID: 12750296] (Rat)
• Park, J et al. Gastric epithelial reactive oxygen species prevent normoxic degradation of hypoxia-inducible factor-1alpha in gastric cancer cells. Clin. Can. Res. 9:433-440. 2003 [PMID: 12538497] (WB, Human)
• Ivan, M et al. Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor. PNAS. 99: 13459-13464. 2002 [PMID: 12351678] (WB, Human)
• Des Tombe AL, Van Beek-Harmsen BJ, Lee-De Groot MB, Van Der Laarse WJ. Calibrated histochemistry applied to oxygen supply and demand in hypertrophied rat myocardium. Microsc Res Tech 2002 Sep 1 [PMID: 12226811] (IHC, Rat)
• Palladino, M et al. Stimulation of hypoxia-inducible factor-1 alpha (HIF-1alpha) protein in the adult rat testis following ischemic injury occurs without an increase in HIF-1alpha messenger RNA expression. Biol. Reprod. 67: 995-1002. 2002 [PMID: 12193413] (WB, Rat)
• Schindl M, Schoppmann SF, Samonigg H et al. Overexpression of hypoxia-inducible factor 1alpha is associated with an unfavorable prognosis in lymph node-positive breast cancer. Clin. Can. Res. 8: 1831-1837. 2002 [PMID: 12060624] (IHC-P, ICC/IF, Human)
• Ivan, M et al. Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling. Science; 292(5516): 464-8. 2001 [PMID: 12004076] (IP)
• Shoshani T, Faerman A, Mett I et al. Identification of a novel hypoxia-inducible factor 1-responsive gene, RTP801, involved in apoptosis. Mol Cell Biol 2002 Apr [PMID: 11884613] (EMSA)
• Pascual O, Denavit-Saubie M, Dumas S et al. Selective cardiorespiratory and catecholaminergic areas express the hypoxia-inducible factor-1alpha (HIF-1alpha) under in vivo hypoxia in rat brainstem. Eur J Neurosci 2001 Dec [PMID: 11860493] (ICC/IF, Rat)
• Arsham AM, Plas DR, Thompson CB, Simon MC. Phosphatidylinositol 3-kinase/Akt signaling is neither required for hypoxic stabilization of HIF-1 alpha nor sufficient for HIF-1-dependent target gene transcription. J Biol Chem 2002 Apr 26 [PMID: 11859074] (WB)
• Stokes DG, Liu G, Coimbra IB et al. Assessment of the gene expression profile of differentiated and dedifferentiated human fetal chondrocytes by microarray analysis. Arthritis Rheum. 2002 Feb [PMID: 11840443] (WB, Bovine)
• Albina JE, Mastrofrancesco B, Vessella JA et al. HIF-1 expression in healing wounds: HIF-1alpha induction in primary inflammatory cells by TNF-alpha. Am J Physiol Cell Physiol 2001 Dec [PMID: 11698256] (Rat)
• Kurebayashi J, Otsuki T, Moriya T et al. Hypoxia reduces hormone responsiveness of human breast cancer cells. Jpn J Cancer Res 2001 Oct [PMID: 11676860] (IHC-P, Human)
• Duyndam, MC et al. Induction of vascular endothelial growth factor expression and hypoxia-inducible factor 1alpha protein by the oxidative stressor arsenite. J Biol Chem;276(51):48066-76. 2001 Dec 21. [PMID: 11641398] (WB, Human)
• Schindl M, Oberhuber G, Pichlbauer EG et al. DNA repair-redox enzyme apurinic endonuclease in cervical cancer: evaluation of redox control of HIF-1alpha and prognostic significance. Int J Oncol 2001 Oct [PMID: 11562758] (Human)
• Birner P, Gatterbauer B, Oberhuber G et al. Expression of hypoxia-inducible factor-1 alpha in oligodendrogliomas: its impact on prognosis on neoangiogenesis. Cancer;92(1):165-71. 2001 Jul 1. [PMID: 11443623] (IHC-P, Human)
• Birner P, Schindl M, Obermair A et al. Expression of hypoxia-inducible factor 1alpha in epithelial ovarian tumors: its impact on prognosis and on response to chemotherapy. Clin Cancer Res 2001 Jun [PMID: 11410504] (IHC, Human)
• Ivan M, Kondo K, Yang H et al. HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing. Science 2001 Apr 20 [PMID: 11292862]
• Jaakkola P, Mole DR, Tian YM et al. Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science 2001 Apr 20 [PMID: 11292861] (Human)
• Birner P, Schindl M, Obermair A et al. Overexpression of hypoxia-inducible factor 1alpha is a marker for an unfavorable prognosis in early-stage invasive cervical cancer. Cancer Res;60(17):4693-6. 2000 Sep 1. [PMID: 10987269] (IHC-P, Human)
• Kamura, T et al. Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex. PNAS 97: 10430-10435. 2000 [PMID: 10973499] (WB, IP)
• Wang G, Hazra TK, Mitra S et al. Mitochondrial DNA damage and a hypoxic response are induced by CoCl(2) in rat neuronal PC12 cells. Nucleic Acids Res 2000 May 15 [PMID: 10773083] (WB)
• Sutter, CH et al. Hypoxia-inducible factor 1alpha protein expression is controlled by oxygen-regulated ubiquitination that is disrupted by deletions and missense mutations. PNAS. 97: 4748-4753. 2000 [PMID: 10758161] (WB, Human)
• Gothie, E et al. Identification of alternative spliced variants of human hypoxia-inducible factor-1alpha. J. Biol. Chem. 275(10):6922-6927. 2000 [PMID: 10702253] (WB, Human)
• Zhong, H et al. Overexpression of hypoxia-inducible factor 1alpha in common human cancers and their metastases. Can. Res.1999; 59: 5830-5835. 1999 [PMID: 10582706] (IHC-P, Human, Mouse)
• Palladino MA, Powell JD, Korah N et al. Expresion Localization of Hypoxia-Inducible Factor-1 Subunits in the Adult Rat Epididymis. Biol Reprod. 70(4):1121-30. 2004 Apr. [PMID: 1466820]

Reviews for HIF-1 alpha Antibody (NB100-105) (39)

Average Rating: 4.2

(Based on 39 reviews)

We have 39 reviews tested in 5 species: Human, Mouse, Rat, Other, also tested in mouse and rat.

reviewed by: Anonymous WB Human 06/13/2019

Summary

• Application: Western Blot
• Sample Tested: Blood mononuclear cells (PBMCs)
• Species: Human
• Lot: BH-13

Comments

• Comments: Confirmation of induction of hypoxia in CoCl2 treated PBMCs infected with vGPCR frame shift knock out and wild type revertant KSHV virion. KSHV infected cells were treated with CoCl2 for 48 hours followed by protein isolation and western blot against HIF1 alpha.

reviewed by: Anonymous WB Human 01/29/2019

Summary

• Application: Western Blot
• Sample Tested: human adrenal cancer cells (whole cell lysate)
• Species: Human
• Lot: unknown

reviewed by: Anonymous WB Rat 01/29/2019

Summary

• Application: Western Blot
• Sample Tested: Rat liver
• Species: Rat
• Lot: AW-1

Comments

• Comments: Detection Method: ECL

reviewed by: Anonymous WB Human 01/24/2019

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: AB7

Comments

• Comments: ECL solution for 5min, then exposure to film 3-5min

reviewed by: Anonymous WB Human 01/23/2019

Summary

• Application: Western Blot
• Sample Tested: 293t cell lysate
• Species: Human
• Lot: NB100-105

reviewed by: Anonymous WB Human 06/29/2017

Summary

• Application: Western Blot
• Sample Tested: 293t cell lysate
• Species: Human
• Lot: bh-1

reviewed by: Anonymous WB Mouse 02/01/2017

Summary

• Application: Western Blot
• Sample Tested: CD4 T cells cultured in vitro, stimulated with anti-CD3/anti-CD28
• Species: Mouse

Comments

• Comments: Figure Legend: Naïve CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1a–deficient (Hif1a KO) mice were differentiated under IL-22–skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1a and lamin B1 levels were analyzed by Western blot.

reviewed by: Anonymous also tested in mouse and rat 12/03/2016

Summary

• Sample Tested: 2 Sample tested
• Species: also tested in mouse and rat
• Lot: N/A

reviewed by: michael dodd WB Mouse 08/17/2016

Summary

• Application: Western Blot
• Sample Tested: HL-1 cardiomyocytes
• Species: Mouse

Blocking

• Blocking Details: 5% milk, in TBST for 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: 1 in 500, Overnight at 4C, in 5% milk with TBST

Secondary Antibody

• Secondary Description: Goat anti-mouse, HRP
• Secondary Concentration: 1 in 2000

Details

• Detection Notes: ECL Prime, Licor scanner, 12 min exposure

reviewed by: Anonymous WB Human 05/08/2016

Summary

• Application: Western Blot
• Sample Tested: k562 cells
• Species: Human
• Lot: BA-3

Blocking

• Blocking Details: 2hs,milk

Primary Anitbody

• Dilution Ratio: 1/500

Secondary Antibody

• Secondary Concentration: 1/2000

Details

• Detection Notes: 45s

reviewed by: Anonymous WB Human 04/18/2016

Summary

• Application: Western Blot
• Sample Tested: HEK293A whole cell lysate
• Species: Human
• Lot: AW-1

Blocking

• Blocking Details: 4% milk in TBST, 1hr at room temperature

Primary Anitbody

• Dilution Ratio: 1:2000 dilution in TBST buffer, incubated at 4 degrees overnight (~16hrs)

Secondary Antibody

• Secondary Description: Goat anti-mouse IgG
• Secondary Manufacturer Cat#: ThermoScientific #31430
• Secondary Concentration: 1:7500

Details

• Detection Notes: Bio-Rad Versadoc, exposure time was one image every 15s for 5 minutes, negative control was empty plasmid

reviewed by: MENG xing WB Mouse 03/24/2016

Summary

• Application: Western Blot
• Species: Mouse
• Lot: BD-10
• Special Applications: Bone marrow cells
• File: View PDF

reviewed by: Anonymous WB Mouse 10/07/2015

Summary

• Application: Western Blot
• Sample Tested: Cell lysate
• Species: Mouse

Blocking

• Blocking Details: Milk

Primary Anitbody

• Dilution Ratio: Overnight 1:500

Secondary Antibody

• Secondary Description: anti-rabbit

Details

• Detection Notes: ECL (doesn't work on Licor)

reviewed by: Zhongxiao Wang WB Mouse 10/05/2015

Summary

• Application: Western Blot
• Sample Tested: mouse retina
• Species: Mouse
• Lot: NB100-479

Blocking

• Blocking Details: 5%milk/T-BST buffer, room temparature, 2hr

Primary Anitbody

• Dilution Ratio: 1:1000 in 5%milk/T-BST buffer, 4 degree, overnight

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG, HRP
• Secondary Manufacturer Cat#: Sigma AQ132P
• Secondary Concentration: 1:3000

Details

• Detection Notes: Develop machine, 30s exposure shows clear band

Comments

• Comments: There's a non-specific band above for mouse retina samples

reviewed by: Anonymous WB Human 08/23/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

Blocking

• Blocking Details: Blocking with 5% non-fat milk for 30 min

Primary Anitbody

• Dilution Ratio: 1:500, overnight

Secondary Antibody

• Secondary Description: Sheep anti-mouse IgG, HRP
• Secondary Manufacturer Cat#: GE Healthcare, NA931V
• Secondary Concentration: 1:2000

Details

• Detection Notes: Regular Protocol

reviewed by: Anonymous WB Human 08/10/2015

Summary

• Application: Western Blot
• Sample Tested: Human prostate cancer cell line protein samples
• Species: Human

Blocking

• Blocking Details: 5% milk in TBS buffer supplemented with 0.1% tween20 at room temperature for one hour

Primary Anitbody

• Dilution Ratio: 1:1000

Secondary Antibody

• Secondary Description: Anti-Rabbit secondary diluted at 1:3000 in 5% milk
• Secondary Manufacturer Cat#: SC-2005
• Secondary Concentration: 0.4 mg/ml

Details

• Detection Notes: Western blotting using ECL reagent (purchased from GE life sciences) and x-ray films.

reviewed by: Anonymous WB Human 04/28/2015

Summary

• Application: Western Blot
• Species: Human
• Special Applications: human hepatocytes from cancer patient
• File: View PDF

reviewed by: Anonymous WB Mouse 12/17/2014

Summary

• Application: Western Blot
• Sample Tested: See PIMD: 23148210
• Species: Mouse

Blocking

• Blocking Details: See PIMD: 23148210

Details

• Detection Notes: See PIMD: 23148210

Comments

• Comments: Published in PMID: 23148210

reviewed by: Xiuquan Luo WB Human 07/18/2014

Summary

• Application: Western Blot
• Sample Tested: Caki-1 cell lysate
• Species: Human

Blocking

• Blocking Details: 1xPBST with 5% non-fat milk, RT 1 hour

Primary Anitbody

• Dilution Ratio: 1xpBST with 5% non-fat milk, overnight 4C, 1:1000 dilution

Secondary Antibody

• Secondary Description: Goat anti-mouse IgG-HRP
• Secondary Manufacturer Cat#: sc-2005
• Secondary Concentration: 1:2000 dilution

Details

• Detection Notes: ECL, exposured for 5 min

reviewed by: Anonymous WB Human 07/01/2014

Summary

• Application: Western Blot
• Sample Tested: Hela Cell Lysate
• Species: Human

reviewed by: Jessica Marinello WB Human 05/09/2014

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human

Blocking

• Blocking Details: TBS milk 5% for 1 hour room temperature

Primary Anitbody

• Dilution Ratio: Antibody diluition 1:1000 in TBS milk 5%, incubation time 2 hours room temperature

Secondary Antibody

• Secondary Description: Goat anti-mouse IgG, HRP
• Secondary Manufacturer Cat#: santa cruz

Details

• Detection Notes: ECL plus Western Blotting Detection Kit 5 minutes incubation room temperature, Phosphorimager detection system,

reviewed by: Anonymous WB Mouse 04/28/2014

Summary

• Application: Western Blot
• Sample Tested: mouse brain lysate
• Species: Mouse

Blocking

• Blocking Details: 7% Milk 30 min RT

Primary Anitbody

• Dilution Ratio: 1: 1000, 4 degree overnight in TBST

Secondary Antibody

• Secondary Description: Goat anti-mouse HRP
• Secondary Manufacturer Cat#: NBP1-47596
• Secondary Concentration: 1ug/mL

reviewed by: Lindsy Dickerson WB Mouse 04/28/2014

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

reviewed by: Ewa Kaniewska IHC Other 04/14/2014

Summary

• Application: Immunohistochemistry
• Species: Other
• File: View PDF

reviewed by: Ewa Kaniewska IF Other 04/14/2014

Summary

• Application: Immunofluorescence
• Species: Other
• File: View PDF

reviewed by: Darlah Lopez WB Mouse 03/28/2014

Summary

• Application: Western Blot
• Species: Mouse
• Special Applications: Primary 1:1000Secondary 1: 10000
• File: View PDF

reviewed by: Hua Li WB Human 08/23/2012

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Human
• Lot: AB7

Blocking

• Blocking Details: 5% milk in TBS-T buffer, room temperature for one hours

Primary Anitbody

• Dilution Ratio: 1:500 in 5% milk -TBS-T solution, 4C for overnight

Secondary Antibody

• Secondary Description: goat anti-mouse IgG, HRP
• Secondary Manufacturer Cat#: Santa Cruz, Cat# sc-2005
• Secondary Concentration: 1:4000

Details

• Detection Notes: ECL solution for 5min, then exposure to film 3-5min

reviewed by: Anonymous IF Rat 08/22/2012

Summary

• Application: Immunofluorescence
• Sample Tested: RAT KIDNEY
• Species: Rat
• Lot: F2
• Comments: PMID 21270095

Blocking

• Blocking Details: serum blocking for 30 minutes.

Primary Anitbody

• Dilution Ratio: 1:100, overnight incubation,room temperature.

Details

• Detection Notes: 1:1000 Alexa Flour 488, 3:1000 Hoechst 33342.
• Fixation Details: 70% acetone 30% methanol.
• Wash Description: 1xPBS, 5 minutes each,3 times.

Comments

• Comments: PMID 21270095

reviewed by: Camilla Sampaoli WB Human 08/22/2012

Summary

• Application: Western Blot
• Sample Tested: human adrenal cancer cells (whole cell lysate)
• Species: Human

Blocking

• Blocking Details: 5% non fat dry milk in PBS 1x + 0,1% tween; 1 h at RT

Primary Anitbody

• Dilution Ratio: 1:500; 5% non fat dry milk in PBS 1x + 0,1% tween; o.n. at +4°C

Secondary Antibody

• Secondary Description: goat anti-rabbit HRP conjugated
• Secondary Manufacturer Cat#: Sigma-Aldrich
• Secondary Concentration: 1:4000; 1 h at RT

Details

• Detection Notes: enhanced chemiluminescence (ECL) assay; exposure: 30 min

reviewed by: Haofeng Ji WB Mouse 05/15/2012

Summary

• Application: Western Blot
• Sample Tested: Cell lysate
• Species: Mouse

Blocking

• Blocking Details: 10% milk

Primary Anitbody

• Dilution Ratio: 1:500

Secondary Antibody

• Secondary Description: anti-rat IgG
• Secondary Concentration: 1:2000

Details

• Detection Notes: ECL

reviewed by: Claudia Di Serio WB Human 05/11/2012

Summary

• Application: Western Blot
• Sample Tested: Human prostate cancer cells
• Species: Human
• Lot: AB1
• Comments: A good result of Novus' HIF-1 alpha Antibody (NB100-105) was obtained when we used a cell lysate with a plate (10 cm) of LNCaP. The cell line and the exposure time of plate was 20 minutes. We realized an immunoreactive band of about 120 KDa.

Blocking

• Blocking Details: 5% dry milk inTBS 1X/tween (0.1%) buffer

Primary Anitbody

• Dilution Ratio: 1:500, 4 Celcius, O/N

Secondary Antibody

• Secondary Description: anti-mouse HRP conjugated
• Secondary Manufacturer Cat#: Pierce Biotechnology
• Secondary Concentration: 1:2000

Details

• Detection Notes: Immunoreactive bands were visualized by enhanced chemiluminescence (ECL) assay

Comments

• Comments: A good result of Novus' HIF-1 alpha Antibody (NB100-105) was obtained when we used a cell lysate with a plate (10 cm) of LNCaP. The cell line and the exposure time of plate was 20 minutes. We realized an immunoreactive band of about 120 KDa.

reviewed by: Anonymous WB Rat 12/14/2011

Summary

• Application: Western Blot
• Sample Tested: Rat liver
• Species: Rat

Blocking

• Blocking Details: Blocking Buffer: 5% non-fat milk in TBST

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:500, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse, Secondary Ab Dilution Ratio: 1:10000

Details

• Detection Notes: Detection Method: ECL

reviewed by: Anonymous WB Mouse 12/09/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse auditory cultured cell, Sample Amount: 30ug
• Species: Mouse
• Comments: HIF-1 alpha degradation was quick, and our result was still detected by your antibody.

Blocking

• Blocking Details: Blocking Buffer: 1% BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse HRP

Details

• Detection Notes: Detection Method: Millipore ECL

Comments

• Comments: HIF-1 alpha degradation was quick, and our result was still detected by your antibody.

reviewed by: Anonymous WB Human 11/21/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

Blocking

• Blocking Details: Blocking Buffer: 5% non-fat milk in TBST

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: overnight

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse

Details

• Detection Notes: Detection Method: Wesern Lighting plus ECL

reviewed by: Lu-Hai Wang ICC Human 11/15/2011

Summary

• Application: Immunocytochemistry
• Sample Tested: Human tissues
• Species: Human
• Comments: It's specific, but not very intense under current expected conditions.

Blocking

• Blocking Details: Blocking Buffer: BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: overnight

Secondary Antibody

• Secondary Description: Secondary Ab: Goat anti-mouse IgG [Alexa Flour 594]

Details

• Detection Notes: Detection Visualization Method: Fluorescence microscopy

Comments

• Comments: It's specific, but not very intense under current expected conditions.

reviewed by: Anonymous WB Human 09/30/2011

Summary

• Application: Western Blot
• Sample Tested: MCF-7, Sample Amount: 100ug
• Species: Human
• Comments: HIF-1 alpha has a half-life of approximately 1-5 min in normoxic conditions, so sometimes the failure to detect its protein is due to the postpone during the sample collection. I strongly suggest all the procedures completed entirely on ice and quickest lysis, processing and storage. Even though, this good antibody is still highly recommended. Cells from MCF-7 grown under normoxia or hypoxia were lysed for HIF-1 alpha blotting. For the best signal, we suggest higher amount of protein (80-120ug) and traditional tank (wet) electroblotting protocol. 10v overnight transferring is the longest but most efficient way to transfer big proteins as Hif-1a. 1:500 dilution of this Ab and 1:2000 secondary Ab work well.
• Pub Med ID: 21193031

Blocking

• Blocking Details: Blocking Buffer: 5% Milk in TBST, Blocking Time: 2 hours, Blocking Temp: 20 degrees Celsius

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:500, Incubation Dilution Buffer: 5% milk TBST, Incubation Time: 16 hours, Incubation Temp: 4 C

Secondary Antibody

• Secondary Description: Secondary Ab: HRP labeled anti-mouse ab, Secondary Ab Dilution Ratio: 1:2000
• Secondary Manufacturer Cat#: 7076 from cell signaling

Details

• Detection Notes: Detection Method: HRP, Exposure Time: 1 minute, Specific Bands: 90 kDa

Comments

• Comments: HIF-1 alpha has a half-life of approximately 1-5 min in normoxic conditions, so sometimes the failure to detect its protein is due to the postpone during the sample collection. I strongly suggest all the procedures completed entirely on ice and quickest lysis, processing and storage. Even though, this good antibody is still highly recommended. Cells from MCF-7 grown under normoxia or hypoxia were lysed for HIF-1 alpha blotting. For the best signal, we suggest higher amount of protein (80-120ug) and traditional tank (wet) electroblotting protocol. 10v overnight transferring is the longest but most efficient way to transfer big proteins as Hif-1a. 1:500 dilution of this Ab and 1:2000 secondary Ab work well.

reviewed by: Anonymous WB Rat 09/01/2010

Summary

• Application: Western Blot
• Sample Tested: endothelial cell nuclear extract, Sample Amount: 40ug
• Species: Rat
• Comments: Good results using this Ab for Western blots and ChIP analysis.

Blocking

• Blocking Details: Blocking Buffer: PBS + 5% NFDM + 0.05% Tween 20, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:250, Incubation Dilution Buffer: PBS + 5% NFDM + 0.05% Tween 20, Incubation Time/Temp: overnight at 4C

Secondary Antibody

• Secondary Description: Secondary Ab: GaM=HRP, Secondary Ab Dilution Ratio: 1:7500

Details

• Detection Notes: Method: Chemiluminescence, Exposure Time: 15 min, Positive Control: nuclear extract from same cells, Specific Bands: 60, 98 kDa

Comments

• Comments: Good results using this Ab for Western blots and ChIP analysis.

reviewed by: William Lin WB Mouse 05/17/2010

Summary

• Application: Western Blot
• Sample Tested: mouse cortical neurons, Sample Amount: 40ug
• Species: Mouse
• Lot: AA1
• Comments: I tested this Hif1a antibody on mouse primary cortical neuronal culture treated with 100uM DFO for over 4 hours. I collected the total lysate using RIPA buffer with 0.1% SDS, sonicated the lysates, and heated up to 90ºC for 5 minutes in SDS-containing sample buffer before loading on an SDS-PAGE gel. I used 1:1000 dilution for the Hif1a antibody (overnight), and 1:20000 anti-mouse secondary antibody for 1 hour. With a few background bands, this antibody works well to detect the upregulation of Hif1a under hypoxic conditions.

Blocking

• Blocking Details: Blocking Buffer: 5% milk in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:1000, Incubation Dilution Buffer: blocking buffer, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse IgG-HRP, Secondary Ab Dilution Ratio: 1:20000
• Secondary Manufacturer Cat#: Promega W402B

Details

• Detection Notes: Detection Method: ECL, Exposure Time: 2 min, Neg.Control: untreated neurons, Specific Bands: 120 kDa, Non-Specific Bands: 67 kDa

Comments

• Comments: I tested this Hif1a antibody on mouse primary cortical neuronal culture treated with 100uM DFO for over 4 hours. I collected the total lysate using RIPA buffer with 0.1% SDS, sonicated the lysates, and heated up to 90ºC for 5 minutes in SDS-containing sample buffer before loading on an SDS-PAGE gel. I used 1:1000 dilution for the Hif1a antibody (overnight), and 1:20000 anti-mouse secondary antibody for 1 hour. With a few background bands, this antibody works well to detect the upregulation of Hif1a under hypoxic conditions.

reviewed by: Anonymous ICC Other 02/10/2010

Summary

• Application: Immunocytochemistry
• Sample Tested: HUVEC & Candida albicans
• Species: Other
• Lot: Z5
• Comments: Works fine with HUVEC but not when Candida albicans is present. The Candida hyphae bind the Ab w/ much greater affinity than the EC and act as a sink leaving the EC completely unstained.

Blocking

• Blocking Details: Blocking Buffer: PBST +1% BSA, Blocking Time: 30 minutes, Blocking Temp: 25 degrees Celsius

Primary Anitbody

• Dilution Ratio: Incubation Dilution Buffer: PBST + 1% BSA, Dilution Ratio: 1/100, Incubation Time: 18 hours, Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse IgG, Secondary Ab Dilution: 1/1000
• Secondary Manufacturer Cat#: Invitrogen A11004

Comments

• Comments: Works fine with HUVEC but not when Candida albicans is present. The Candida hyphae bind the Ab w/ much greater affinity than the EC and act as a sink leaving the EC completely unstained.

FAQs for HIF-1 alpha Antibody (NB100-105). (Showing 1 - 10 of 30 FAQs).

1. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
   • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
2. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
   • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).
3. I would like to know, does a path exist for detection of HIF 1 in venous blood before and after revascularization of the leg? 
   • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present, but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
4. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
   • The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.
5. We got the Hif1a (https://www.novusbio.com/products/hif-1-alpha-antibody-h1alpha67_nb100-105 ) antibody from you guys. I used the concentration that is mentioned on your website, but I am getting a band of a completely different size (~70kDa) and not the 120 kDa mentioned.
   • HIF-1 alpha is a notoriously difficult protein to work with due to its rapid degradation. Therefore, the ~70kDa bands are most likely degradation products. It is very important to lyse the cells in hypoxic conditions. We strongly recommend lysing the cells directly into the Laemmli buffer and doing that quickly, so that the exposure to oxygen is minimized.Please go through our hypoxia related FAQs, you should find them very informative:https://www.novusbio.com/support/hypoxia-and-hif-faqsAlso, running a positive control may help confirm the band specificity in your samples. You may prepare them yourself or choose some from our catalog, for example: 1) HeLa Hypoxic / Normoxic Cell Lysate (NBP2-36452)2) HeLa Hypoxic (CoCl2) / Normoxic Cell Lysate (NBP2-36450)
6. I performed several Western Blots of HIF-1 alpha with different lysis buffers, whole lysates, and cytoplasm/nuclei extractions. I can’t seem to get a good western blot (poor signal, band much lower than expected, etc.). Can someone suggest some technical considerations/tricks I should consider using?
   • A major issue that researchers working with HIF-1 alpha is degradation due to exposure to oxygen. In western blot, this results in a weaker band and/or the appearance of multiple low molecular weight bands (40-80 kDa). We recommend preparing the lysates after collection of cells/tissues as quickly as possible (on ice), preferably in a hypoxic chamber. We also recommend including a true hypoxia mimetic control (eg: cells treated with CoCl2, DMOG… etc.). The controls help distinguish your band of interest from potential degradation/dimer bands.For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs: https://www.novusbio.com/support/hypoxia-and-hif-faqs
7. I am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. Can someone let me know if I’m getting the right HIF band or just some non-specific bands? Thanks.
   • (1)    HIF-1 alpha’s theoretical molecular weight is 93kDa. The post translationally modified/ubiquitinated form of HIF-1 alpha protein (fails to undergo proteasomal degradation) shows up as a band in the 110-130 kDa range on a Western blot.(2)    The dimeric protein may appear at a position above 200 kDa on non-reducing gels.(3)    Importantly, HIFs are among the most rapidly degradable proteins; therefore, sample preparation is highly important when analyzing HIF1 alpha or HIF2 alpha. When degraded, HIF-1 alpha may show up between 40-80 kDa position on Western blot. Degradation may be avoided by preparing the samples as soon as possible after collection of cells/tissues in hypoxic chamber. Notably, the tissues/cells should be kept on ice during lysate preparation and the lysates should be analyzed as soon as possible.(4)    For troubleshooting suggestions/feedback on more than 25 similar frequently asked questions, I would recommend visiting Novus page: FAQs - Hypoxia and HIFs https://www.novusbio.com/support/hypoxia-and-hif-faqs(5)    Last but not the least, Novus technical support team may be contacted at: technical@novusbio.com 
8. I have Hif1a nuclear protein extract at -80C. I am wondering if anyone knows how long it would be good for at that temperature since HIf1a is known to be degraded easily.Thank you!
   • You could try a few things to further inhibit the degradation.1) Use the protease inhibitors (if you are not already using them).2) Lyse cells into a buffer that contains SDS or LDS (eg: Laemmli's buffer), since SDS and LDS denature and inhibit proteases. Lysis may even be performed with reducing agents in the buffer (eg. DTT), but this will make your lysates unsuitable for BCA assay.3) Lysing samples rapidly ensures that the samples are instantly homogenized (it also shears DNA released by the SDS).5) Flash-freezing samples in liquid nitrogen rather than freezing at -80*C reduces the window of time for protease activity.6) Freeze samples in individual aliquots, instead of thawing the same vial multiple times.
9. I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
   • CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs: http://www.novusbio.com/support/hypoxia-and-hif-faqs
10. I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
    • CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs: https://www.novusbio.com/support/hypoxia-and-hif-faqs
11. Is cross-reactivity with HIF-2 alpha tested/predicted?
    • Although we don’t have cross-reactivity data with regards to HIF-2 alpha, we predict minimal cross-reactivity based on low sequence similarity observed from BLAST analysis between HIF-1 alpha and HIF-2 alpha.
12. Do you have any suggested IHC Protocol for this antibody? We had been trying to validate protocols from other research studies but failed to get good results. Background staining seems to be the problem. 
    • Yes, we have a specific IHC protocol for NB100-105. It is located on the datasheet under the "Protocols & FAQs" tab. We also have over 300 publications that utilize this product. If you need help finding a specific paper or if you would like us to help troubleshoot your experiment, please contact Technical Support at technical@novusbio.com.
13. Has anyone used this antibody for supershft in EMSA?
    • Yes, this antibody is useful for EMSA and has been documented in couple of recent research publications. You may refer to the following paper for details: Kong D, et al. Echinomycin, a small-molecule inhibitor of hypoxia-inducible factor-1 DNA-binding activity. Cancer Res. 2005 Oct 1;65(19):9047-55 [PMID: 16204079].
14. What is the difference in the following two HIF-1 alpha antibodies? Catalog # NB100-105 and NB100-134
    • NB100-105 is a mouse monoclonal antibody, whereas NB100-134 is a rabbit polyclonal. They both use the same immunogen.
15. I am working on a WB using the antibody against mouse HIF1a and HIF2a (catalog numbers NB100-105 and NB100-122). I tried several anti-mouse and anti-rabbit HRP secondary antibodies (from another company) and found they gave different patterns. Please provide the secondary antibody info which was used in the WB showed on the datasheet.
    • We use catalog numbers NB7570 and NB7183 as our secondary antibodies for testing these antibodies in WB.
16. What is the difference between NB100-105 and NB100-123?
    • NB100-105 and NB100-123 are similar as far as immunogens and clones are concerned. However, they are different with respect to their purification process, concentrations and recommended dilutions. NB100-123 is Protein A purified IgG2b with a concentration of 1.5 mg/ml, whereas NB100-105 is Protein G purified IgG2b with 4.0 mg/ml concentration. Another difference is the recommended dilutions (because of the differences in their concentration).
17. Can I get a sample of the HIF1A antibody (NB100-105)?
    • We do offer this antibody in a sample size which contains 0.025ml of antibody. The sample size catalog number is NB100-105SS.
18. I did staining of 150uM Cobalt chloride induced 3T12 for 24 hours using this HIF1alpha antibody. I used a 1:50 dilution for one hour at room temperature. Should I do an overnight incubation?
    • We do recommend incubating samples overnight at 4 degrees Celsius for the best results.
19. Is there a product available to be used as the blocking peptide of NB100-105?
    • We unfortunately do not have a blocking peptide for NB100-105. It was raised against a fusion protein. We also unfortunately do not sell the protein.
20. Could you advise if I can use NBP1-96969 Ab as a control Ab for HIF1a for ChIP assay. I have a problem that normal rabbit IgG that usually are provided in the ChIP kits show higher binding compare to NB100-105 HIF1A Ab. Could you suggest if it is better to use Mouse IgG 2b Isotype control in this case or normal rabbit IgG should work as well? NBP1-96969 Ab are not validated for ChIP, please, let me know if there is something better? 
    • Yes, it would be reasonable to use a mouse IgG2b control for NB100-105 (HIF1 alpha) in ChIP assay. The control provided in the ChIP kit is recommended as a control for the histone antibodies (which are rabbit polyclonal). NBP1-96969 would be a good choice for control for the mouse monoclonal NB100-105. It has not yet been validated for ChIP, but it has been validated for use in IP, so I think it will be a good choice for control.
21. Since HIF-1 is nuclear protein, why is there obvious staining in the cytoplasm, which is displayed in the IHC image on the product website. Can you be so kind to help me? 
    • HIF1-alpha is found at very low levels in the cytoplasm under normoxic conditions. During hypoxia, HIF1-alpha is stabilized and translocates to the nucleus to act as a transcription factor. You would therefore expect to see nuclear staining in your hypoxic samples, but may also observe faint cytoplasmic staining in relation to degraded HIF in the samples. HIF proteins are notoriously difficult to work with, as they degrade very rapidly. The cytoplasmic staining is a result of HIF1-alpha degradation. I hope that this helps. You may find our guide to working with hypoxia antibodies to be useful.
22. I would like to know why I have to dilute 1:500 the primary antibody. How can I know which is the best dilution (or what amount of antibody I need to take) to my sample in the WB?
    • Our recommended dilution for use of NB100-105 in western blot is 1:500. You may need to adjust this dilution depending on the signal you are able to detect in your samples. I would recommend using a titer with 1:500 as the starting point to find the optimal dilution to use in your experiment.
23. I am wondering if you sell a blocking peptide for the HIF1a antibody cat # NB100-105?
    • We unfortunately do not sell the blocking immunogen for our product NB100-105. I am sorry for any inconvenience.
24. Could you tell me the epitope of #NB100-105?
    • We seldom map our antibodies, including monoclonals. Therefore, we do not know where this monoclonal antibody binds to in HIF-1 alpha. However, the region containing the epitope for NB100-105 could be located in the a.a. sequence used as the immunogen for raising the antibody. According to the datasheet, the immunogen for NB100-105 was: "... amino acids 432-528 of human HIF-1 alpha. [UniProt# Q16665]."
25. Would you be able to share the concentration of NB100-105 – HIF-1 alpha mAb from Lot# AB1?
    • The concentration of NB100-105 Lot AB1 is 4.0mg/ml.
26. Could you tell us if these antibodies need to use cell lysate treated with special extraction for WB? Do you have the recommended preparation method of cell lysate?
    • Usually HIF1 alpha and HIF2 alpha are hard to detect endogenously and require some inducement. The most common is hypoxic treatment of the cells. This can be also be chemically induced by CoCl2 treatment as well. Our recommended protocols and lysate preparations can be found in our white paper here: http://www.novusbio.com/whitepapers/cellular-response-hypoxia.html.
27. Is this antibody used for intracellular staining?
    • NB100-105 detects HIF-1 alpha, which is a nuclear protein, so you would want to consult our intracellular nuclear target flow protocol:https://www.novusbio.com/support/support-by-application/general-intracellular-nuclear-target-flow-protocol
28. I am using HIF1 alpha antibody NB100-105 for Western blot. Do you have any recommendations on the preparation of lysates? Also, what positive control do you suggest for WB with HIF1 alpha antibody NB100-105?
    • HIF1a protein is known to degrade easily in presence of oxygen.  For this reason the conditions for lysate preparation are crucial, we strongly recommend lysing cells quickly under cold condition by putting cell plates on ice and saving the lysates directly into the SDS-loading buffer. If nuclear fraction is desired we recommend using hypotonic buffer. You can see specific instructions for WB with HIF1 alpha antibody NB100-105 at: https://www.novusbio.com/support/protocols/western-blot-protocol-for-hif-1-alpha-antibody-nb100-105.html We recommend lysate from cells cultured in hypoxic conditions or in presence of CoCL2, which stabilizes HIF1a. We also have several controls available on our website: HeLa Hypoxic/Normoxic Cell Lysate: NBP2-36452; HeLa Hypoxic (CoCl2)/Normoxic Lysate: NBP2-36450; etc. Plese see here: https://resources.novusbio.com/manual/Manual-NB100-105-15269044.pdf
29. We see high background in our western blots using GAPDH antibodies H00002483-M01 and NB100-105.
    • To avoid multiple bands, we suggest:1)     Using a nitrocellulose membrane. It produces less background compared to PVDF membranes.2)     Overnight blocking at 4℃.3)     Increasing the wash time. Try washing the membrane for 10 mins, repeating 4 times.
30. NB100-105 has the same clone name (H1alpha67) as NB100-123.Are these the same clone?
    • These are the same clones, however their purifications are slightly different (and thus their recommended dilutions for certain applications are a bit different, please see this section "Applications/Dilutions" on the specific product pages for differences).NB100-105: Protein G purifiedhttps://www.novusbio.com/products/hif-1-alpha-antibody-h1alpha67_nb100-105NB100-123: Protein A purifiedhttps://www.novusbio.com/products/hif-1-alpha-antibody-h1alpha67_nb100-123The basis for purification of IgG, IgG fragments and IgG subclasses is the high affinity of protein A and protein G for the Fc region of polyclonal and monoclonal IgG-type antibodies. Protein A and protein G are bacterial proteins, which, when coupled to chromatography matrix such as Agarose or Sepharose, generate exceptionally useful, easy to use media (resin) for many routine applications. Protein A/G is a recombinant of Protein A and Protein G that has the additive binding properties of both proteins. Protein A, protein G and protein A/G can be used for purification of monoclonal IgG-type antibodies, purification of polyclonal IgG subclasses, and the adsorption and purification of immune complexes involving IgG. IgG subclasses can be isolated from cell culture supernatants and serum and from ascites fluid.Lastly, we have been producing NB100-105 for a longer time period, and thus why it has considerably more publication references (77) than NB100-123 (138)https://www.novusbio.com/products/hif-1-alpha-antibody-h1alpha67_nb100-105#PublicationSectionhttps://www.novusbio.com/products/hif-1-alpha-antibody-h1alpha67_nb100-123#PublicationSection
Show All 30 FAQs.

Bioinformatics

• Gene Symbol: HIF1A
• OMIM:
  • 603348(Human)