Raw 264.7 CoCl2 treated/Untreated Cell Lysate

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Product Details

Summary
Applications PAGE
Concentration
1.0 mg/ml

Order Details

Raw 264.7 CoCl2 treated/Untreated Cell Lysate Summary

Preparation
Method
RAW264.7 cells are grown under standard tissue culture conditions until semi-confluent (70-80%). The cells are then treated with or without 100 uM CoCl2 for 4 hours, washed in PBS and directly lysed into 1X Laemmli sample buffer with BME. The lysate is sonicated and tested by Western blot.

Applications/Dilutions

Dilutions
  • SDS-Page 10 ul / lane
Application Notes
RAW264.7 whole cell lysate is provided as a Western blot positive control. The lysates are provided as a set of treated and untreated samples. Use 10 ul per lane for a standard mini-gel blot (1.0 mg/ml).

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
The protein lysate is prepared in 1x Laemmli sample buffer with BME. Boiling before loading is not necessary.
Concentration
1.0 mg/ml

Lysate Details for Array

Type
Cell
Subcellular Fraction
CoCl2 treated/Untreated

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Additional Raw 264.7 Products

Array NBP3-11857

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