Raw 264.7 CoCl2 treated/Untreated Cell Lysate Summary
RAW264.7 cells are grown under standard tissue culture conditions until semi-confluent (70-80%). The cells are then treated with or without 100 uM CoCl2 for 4 hours, washed in PBS and directly lysed into 1X Laemmli sample buffer with BME. The lysate is sonicated and tested by Western blot.
RAW264.7 whole cell lysate is provided as a Western blot positive control. The lysates are provided as a set of treated and untreated samples. Use 10 ul per lane for a standard mini-gel blot (1.0 mg/ml).
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
The protein lysate is prepared in 1x Laemmli sample buffer with BME. Boiling before loading is not necessary.
Lysate Details for Array
Raw264.7 cells are a macrophage-like, Abelson leukemia virus transformed cell line derived from BALB/c mice. They were established from the ascites of a tumor induced male mouse via intraperitoneal injection of Anselon Leukemia Virus (A-MuLV). Raw264.7 cells are capable of antibody dependent lysis of sheep erythrocytes and tumor targets. Growth of Raw264.7 cells is inhibited by lipopolysaccharides (LPS).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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