p53 Antibody (PAb 240) - BSA Free

Images

 
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # ...read more
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with ...read more
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on MCF7 cells with p53 [PAb 240] Antibody NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on Neuro2a cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ye, Xp(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
PAb 240
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

p53 Antibody (PAb 240) - BSA Free Summary

Immunogen
Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).
Epitope
The epitope has been mapped between amino acids 213 and 217 on human p53. The epitiope may be conformational as it reacts with mutant p53 in nondenatured form.
Localization
Nuclear/cytoplasmic
Specificity
This monoclonal recognizes both mutant forms and wild-type human p53 under denaturing conditions. It does not recognize nondenatured wild type p53. In nondenaturing conditions (IP) it only recognizes mutant p53.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TP53
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:100-1:2000
  • Flow (Intracellular)
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml. Use reported in scientific literature (PMID 1394225)
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Frozen reported in multiple pieces of scientific literature
  • Immunohistochemistry-Paraffin 1:250-1:500
  • Immunoprecipitation 10ug/mg
  • Western Blot 1:1000-1:2000
Application Notes
Antigen retrieval with IHC-P is not essential but may optimize staining. In IP this antibody reacts with only mutant p53 protein under non-denaturing conditions. This antibody is CyTOF ready.
Control
p53 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB200-103 in the following applications:

Publications
Read Publications using
NB200-103 in the following applications:

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 8710879)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for p53 Antibody (PAb 240) - BSA Free

  • Antigen NY-CO-13
  • BCC7
  • FLJ92943
  • LFS1
  • LFS1TRP53
  • p53 tumor suppressor
  • p53
  • P53cellular tumor antigen p53
  • Phosphoprotein p53
  • TP53
  • transformation-related protein 53
  • TRP53
  • tumor protein p53
  • Tumor suppressor p53

Background

The tumor suppressor protein p53 plays an important role in cancer development. The p53 protein is a transcription factor that acts as a checkpoint in the cell cycle, either preventing or initiating programmed cell death. Since cancer occurs as the malfunction in the proliferation of cells, disrupting p53 activity allows abnormal cells to multiply or allows a tumor's progression from benign to malignant. This antibody detects p53 in all mammals tested. Detection of p53 using this antibody: 50% of colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissues are negative.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p53 Antibody (NB200-103)(39)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Yeast.

We have publications tested in 10 applications: ELISA, FLOW, Hydrolysis Assay, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ELISA
(2)
FLOW
(1)
Hydrolysis Assay
(1)
ICC/IF
(4)
IF/IHC
(6)
IHC
(1)
IHC-Fr
(2)
IHC-P
(5)
IP
(5)
WB
(15)
All Applications
Filter By Species
Human
(18)
Mouse
(12)
Rat
(2)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using NB200-103 Applications Species
Reis-Barbosa PH, Marinho TS, Matsuura C et al. The obesity and nonalcoholic fatty liver disease mouse model revisited: Liver oxidative stress, hepatocyte apoptosis, and proliferation Acta histochemica 2022-08-08 [PMID: 35952484] (WB, Mouse) WB Mouse
Yue W, Ma J, Xiao Y et al. The Apoptotic Resistance of BRCA1-Deficient Ovarian Cancer Cells is Mediated by cAMP Frontiers in Cell and Developmental Biology 2022-04-20 [PMID: 35517499]
Kong N, Chen X, Feng J et al. Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1 Acta Pharm Sin B 2022-01-13 [PMID: 35024325]
Setiawati A. Cytotoxic Activity of Anredera cordifolia Leaf Extract on HeLa Cervical Cancer Cells through p53-Independent Pathway Asian Journal of Pharmaceutical and Clinical Research 2015-02-07 (ICC/IF, Human)

Details:
p53 antibody used for immunocytostaining application with DAB detection on HeLa cells treated or not with Anredera cordifolia leaf extract or 40um cisplatin - 5 minutes fixation, 15 minutes incubation with H2O2, 1 hour primary incubation, detection with biotinylated goat anti-polyvalent secondary antibody and DAB staining followed by counter staining with hematoxylin (Fig. 6).
ICC/IF Human
Mansky RH, Greguske EA, Yu D et al. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease Cell reports 2023-03-02 [PMID: 36867535] (IHC-P, WB, Mouse) IHC-P, WB Mouse
Anton PE, Rutt LN, Capper C et al. Profiling the oxylipidome in aged mice after chronic ethanol feeding: Identifying lipid metabolites as drivers of hepatocyte stress Alcohol 2023-03-01 [PMID: 36150611] (IHC) IHC
Radtke AJ, Chu CJ, Yaniv Z Et al. IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues Nat Protoc 2022-01-13 [PMID: 35022622] (Hydrolysis Assay, Human)

Details:
Citation using the Alexa Fluor 532 version of this antibody.
Hydrolysis Assay Human
Fontana MC, Nanni J, Ghelli Luserna di RorA A et al. Pharmacological Inhibition of WIP1 Sensitizes Acute Myeloid Leukemia Cells to the MDM2 Inhibitor Nutlin-3a Biomedicines 2021-04-06 [PMID: 33917342] (WB, Human) WB Human
Marks D, Heinen N, Bachmann L et al. Amyloid precursor protein elevates fusion of promyelocytic leukemia nuclear bodies in human hippocampal areas with high plaque load Acta neuropathologica communications 2021-04-13 [PMID: 33849647] (IF/IHC, Human) IF/IHC Human
Li W, Gu X, Liu C et al. A synergetic effect of BARD1 mutations on tumorigenesis Nature communications 2021-02-23 [PMID: 33623049]
Show All 39 Publications.

Reviews for p53 Antibody (NB200-103) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB200-103:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot p53 NB200-103
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 09/14/2018
View

Summary

ApplicationWestern Blot
Sample TestedC166 mouse cell line
SpeciesMouse
LotG4
  4
reviewed by:
Matt Stratton
WB Rat 03/26/2014
View

Summary

ApplicationWestern Blot
SpeciesRat
LotA
Western Blot p53 NB200-103
Enlarge
5
reviewed by:
Astrid Spannhoff
WB Human 10/13/2010
View

Summary

ApplicationWestern Blot
Sample TestedWhole Cell Lysate of MCF-7 and HeLa cells, Sample Amount: 10 ul
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p53 Antibody (NB200-103) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for p53 Antibody (NB200-103)

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Blogs on p53. Showing 1-10 of 19 blog posts - Show all blog posts.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

Autophagy independent roles of the core ATG proteins
By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation.  It is critical for removal of dam...  Read full blog post.

Killing two birds with one stone: Treating inflammation and cancer by inhibiting prolyl-4-hydroxylase-1
By Jamshed Arslan Pharm.D. The cell’s oxygen-sensing machinery comprises prolyl-4-hydroxylases (P4Hs 1-3, PHDs 1-3, or EGLN 1-3) and their canonical target hypoxia-inducible factors (HIFs). When oxygen levels ...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels ...  Read full blog post.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

Showing 1-10 of 19 blog posts - Show all blog posts.
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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Verified Customer
09/14/2018
Application: WB
Species: Mouse

 
Matt Stratton
03/26/2014
Application: WB
Species: Rat

Astrid Spannhoff
10/13/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol TP53
Entrez