p53 Antibody (PAb 240)

Images

 
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # ...read more
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ye, Xp(-)Species Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
PAb 240
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

p53 Antibody (PAb 240) Summary

Immunogen
Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).
Epitope
The epitope has been mapped between amino acids 213 and 217 on human p53.
Localization
Nuclear
Specificity
This monoclonal recognizes both mutant forms and wild-type human p53 under denaturing conditions.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TP53
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:250-1:500
  • Immunoprecipitation 10ug/mg
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
Antigen retrieval with IHC-P is not essential but may optimise staining. In IP this antibody reacts with only mutant p53 protein under non-denaturing conditions. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 1394225) Use in Immunohistochemistry-Frozen reported in various pieces of scientific literature.
Positive Control
p53 Lysate (NBL1-17203)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB200-103 in the following applications:

Publications
Read Publications using
NB200-103 in the following applications:

Reactivity Notes

Cross-reacts with Human, Mouse, Rat and most other Mammals.Yeast reactivity reported in scientific literature (PMID: 8710879) Does not cross-react with Xenopus laevis. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for p53 Antibody (PAb 240)

  • Antigen NY-CO-13
  • BCC7
  • FLJ92943
  • LFS1
  • LFS1TRP53
  • p53 tumor suppressor
  • p53
  • P53cellular tumor antigen p53
  • Phosphoprotein p53
  • TP53
  • transformation-related protein 53
  • TRP53
  • tumor protein p53
  • Tumor suppressor p53

Background

The tumor suppressor protein p53 plays an important role in cancer development. The p53 protein is a transcription factor that acts as a checkpoint in the cell cycle, either preventing or initiating programmed cell death. Since cancer occurs as the malfunction in the proliferation of cells, disrupting p53 activity allows abnormal cells to multiply or allows a tumor's progression from benign to malignant. This antibody detects p53 in all mammals tested. Detection of p53 using this antibody: 50% of colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissues are negative.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p53 Antibody (NB200-103)(22)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Yeast.

We have publications tested in 8 applications: ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ELISA
(2)
FLOW
(1)
ICC/IF
(3)
IHC
(4)
IHC-Fr
(3)
IHC-P
(2)
IP
(5)
WB
(7)
All Applications
Filter By Species
Human
(13)
Mouse
(7)
Rat
(2)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB200-103 Applications Species
Graham NA, Minasyan A, Lomova A et al. Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures. Mol. Syst. Biol. 2017 Feb 15 [PMID: 28202506] (WB, Mouse) WB Mouse
Shi M, Ma L, Zhou L, Fu P Renal Protective Effects of 17-Estradiol on Mice with Acute Aristolochic Acid Nephropathy. Molecules Jul 8 1905 [PMID: 27763560] (WB, Human) WB Human
Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015 Sep 24 [PMID: 26400209] (IHC, Mouse) IHC Mouse
Biswas AK, Mitchell DL, Johnson DG. E2F1 Responds to Ultraviolet Radiation by Directly Stimulating DNA Repair and Suppressing Carcinogenesis. Cancer Res. 2014 Apr 16 [PMID: 24741006] (IHC-P, Mouse) IHC-P Mouse
Lange SS, Bedford E, Reh S et al. Dual role for mammalian DNA polymerase in maintaining genome stability and proliferative responses. Proc Natl Acad Sci U S A 2013 Feb 5 [PMID: 23386725] (IHC-P, Mouse) IHC-P Mouse
Jensen MR, Factor VM, Fantozzi A et al. Reduced hepatic tumor incidence in cyclin G1-deficient mice. Hepatology. 2003 Apr [PMID: 12668979] (WB, Mouse) WB Mouse
Asher G, Lotem J, Cohen B et al. Regulation of p53 stability and p53-dependent apoptosis by NADH quinone oxidoreductase 1. Proc Natl Acad Sci USA. 2001 Jan 30 [PMID: 11158615] (WB, Mouse) WB Mouse
Jones NA, Turner J, McIIwrath AJ et al. Cisplatin- and paclitaxel-induced apoptosis of ovarian carcinoma cells and the relationship between bax and bak up-regulation and the functional status of p53. Mol Pharmacol. 1998 May [PMID: 9584207] (IP, Human) IP Human
Blagoskionny MV, Toretsky J, Bohen S et al. Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90. Proc Natl Acad Sci USA. 1996 Aug 6 [PMID: 8710879] (IP, Yeast) IP Yeast
Halazonetis TD, davis LJ, Kandil AN. Wild-type p53 adopts a 'mutant'-like conformation when bound to DNA. EMBO J. 1993 Mar [PMID: 8458320] (WB, IP, Human) WB, IP Human
Show All 22 Publications.

Reviews for p53 Antibody (NB200-103) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.
We have 2 review tested in 1 application: WB.

Reviews using NB200-103:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Matt Stratton
WB Rat 03/26/2014
View

Summary

ApplicationWestern Blot
SpeciesRat
LotA
Special Applicationsvalidated using shRNA targeting p53</p><p> COSAMPLES
FileView PDF
Western Blot p53 NB200-103
Enlarge
reviewed by:
Astrid Spannhoff
WB Human 10/13/2010
View

Summary

ApplicationWestern Blot
Sample TestedWhole Cell Lysate of MCF-7 and HeLa cells, Sample Amount: 10 ul
SpeciesHuman
Commentstested on MCF-7 cells lysate and Hela cell lysate as a control for a new project

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP-labeled anti-mouse goat IgG, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#NA931

Details

Detection NotesDetection Method: ECL1, Exposure Time: 30 seconds, Positive Control: both cell lines, Specific Bands: 53 kDa

Comments

Commentstested on MCF-7 cells lysate and Hela cell lysate as a control for a new project

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p53 Antibody (NB200-103) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-103AF405
Alexa Fluor 488 Labeled NB200-103AF488
Alexa Fluor 647 Labeled NB200-103AF647
Alexa Fluor 700 Labeled NB200-103AF700
Allophycocyanin Labeled NB200-103APC
DyLight 350 Labeled NB200-103UV
DyLight 405 Labeled NB200-103V
DyLight 488 Labeled NB200-103G
DyLight 680 Labeled NB200-103FR
DyLight 755 Labeled NB200-103IR
FITC Labeled NB200-103F
HRP Labeled NB200-103H
PE Labeled NB200-103PE
PerCP Labeled NB200-103PCP

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Blogs on p53. Showing 1-10 of 16 blog posts - Show all blog posts.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

PRMT6: One Function, Many Roles
Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

p14 ARF is an Important Tumor Suppressor
The p14ARF (Alternative Reading Frame) tumor suppressor is a protein product of the alternative reading frame (ARF) of the human INK4a locus which regulates a series of cell cycle regulatory proteins to promote cell cycle arrest in response to abnorma...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Matt Stratton
03/26/2014
Application: WB
Species: Rat

Astrid Spannhoff
10/13/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol TP53
Entrez