ARNT/HIF-1 beta Antibody (H1beta234)

Images

 
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Analysis of HIF-1 beta in HeLa nuclear extract.
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. ...read more
Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Staining of human glioblastoma multi-forme utilizing anti-HIF-1 Beta.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ft, Pm, ShSpecies Glossary
Applications WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP
Clone
H1beta234
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.4 mg/ml

ARNT/HIF-1 beta Antibody (H1beta234) Summary

Immunogen
Fusion protein containing amino acids 496-789 of human HIF-1 beta. [UniProt# P27540]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ARNT
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
Application Notes
This HIF-1 beta (H1beta234) antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections and Chromatin Immunoprecipitation. By Western blot a band at approximately 92 kDa is seen. Use in Gel Shift Super Assay reported in scientific literature (PMID: 11325839).Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25343232) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
ARNT/HIF-1 beta Lysate (NBL1-07720)
Reviewed Applications
Read 1 Review rated 5
using
NB100-124 in the following applications:

Publications
Read Publications using
NB100-124 in the following applications:

  • 14 publications
  • GS
    1 publication
  • IB
    16 publications
  • 2 publications
  • IHC
    4 publications
  • 2 publications
  • IP
    6 publications
  • WB
    48 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.4 mg/ml
Purity
Protein G purified

Alternate Names for ARNT/HIF-1 beta Antibody (H1beta234)

  • ARNT protein
  • ARNT
  • aryl hydrocarbon receptor nuclear translocator
  • BHLHE2
  • HIF1 beta
  • HIF-1 beta
  • HIF1B
  • HIF1BETA
  • HIF-1-beta
  • HIF1-beta
  • hypoxia-inducible factor 1, beta subunit
  • Hypoxia-inducible factor 1-beta
  • nuclear translocator
  • TANGO

Background

HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits which are bHLH proteins of PAS (PER, ARNT, SIM) family. HIF-1 beta forms heterodimer with AHR, AHRR, HIF1 alpha and EPAS1/HIF2 alpha as well as with other bHLH proteins and its dimerization is required for efficient DNA binding. It has been shown to interact with TACC3 and NOCA7 also. HIF-1 beta is also known as ARNT (AhR nuclear translocator) due to its ability to partner with AhR to form a heterodimeric transcription factor complex which is required for AhR activity. It is essential for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding and the AhR-HIF-1 beta complex then initiates transcription of genes involved in xenobiotic transformation, especially of PAHs. HIF-1 beta heterodimer with HIF1 alpha or EPAS1/HIF2 alpha functions as a transcriptional regulator of the adaptive response to hypoxia. Moreover, a chromosomal translocation resulting in TEL-ARNT fusion protein is associated with AML (acute myeloblastic leukemia) and decreased HIF-1 beta expression levels in pancreatic islets from NIDDM subjects reflects its important role in beta-cell function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Ft, Pm, Sh
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP

Publications for ARNT/HIF-1 beta Antibody (NB100-124)(89)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ChIP, GS, IB, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ChIP
(14)
GS
(1)
IB
(16)
ICC/IF
(2)
IHC
(4)
IHC-P
(2)
IP
(6)
WB
(48)
All Applications
Filter By Species
Human
(62)
Mouse
(10)
Rat
(7)
All Species
Showing Publications 1 - 10 of 89. Show All 89 Publications.
Publications using NB100-124 Applications Species
Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca2+ Release Stimulates Breast Cancer Stem Cell Enrichment. Cell Rep. [PMID: 28228260] (WB, ChIP, Human) WB, ChIP Human
Murakami A, Wang L, Kalhorn S et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma Oncogenesis Jan 16 2017 [PMID: 28092369] (WB, Human) WB Human
Taylor SE, Bagnall J, Mason D et al. Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility Open Biol Sep 1 2016 [PMID: 27655733] (ICC/IF, Human) ICC/IF Human
Thienpont B, Steinbacher J, Zhao H et al. Tumour hypoxia causes DNA hypermethylation by reducing TET activity. Nature. Aug 17 2016 [PMID: 27533040] (ChIP, Mouse) ChIP Mouse
Li S, Heller JJ, Bostick JW et al. Ikaros Inhibits Group 3 Innate Lymphoid Cell Development and Function by Suppressing the Aryl Hydrocarbon Receptor Pathway Immunity 2016 Jul 19 [PMID: 27438771] (WB, Human) WB Human
Luo W, Chen I, Chen Y et al. PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia. Oncotarget 2016 Feb 9 [PMID: 26837221] (WB) WB
Altuntas S, Rossin F, Marsella C et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget 2015 Dec 29 [PMID: 26702927] (WB) WB
Frey JL, Stonko DP, Faugere MC, Riddle RC Hypoxia-inducible factor-1alpha restricts the anabolic actions of parathyroid hormone. Bone Res 2014 May 13 [PMID: 26273518] (IP) IP
Xiang L, Gilkes DM, Hu H et al. HIF-1alpha and TAZ serve as reciprocal co-activators in human breast cancer cells. Oncotarget. 2015 May 20 [PMID: 26059435] (IP, ChIP, WB, Human) IP, ChIP, WB Human
Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014 [PMID: 25587023] (ChIP, Human) ChIP Human
Show All 89 Publications.

Review for ARNT/HIF-1 beta Antibody (NB100-124) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-124:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Lin Du
WB Human 01/04/2011
View

Summary

ApplicationWestern Blot
Sample TestedT47D cell nuclear extract
SpeciesHuman

Product General Protocols

View specific protocols for ARNT/HIF-1 beta Antibody (NB100-124): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for ARNT/HIF-1 beta Antibody (NB100-124). (Showing 1 - 3 of 3 FAQs).

  1. Does your guarantee to apply WB of NB100-124?
    • For NB100-124, we have the following Western Blot Procedure: 1. Resolve aliquots (15 mg) of induced nuclear protein extracts on a SDS/6% polyacrylamide gel. 2. Transfer to nitrocellulose membranes in 20 mM Tris-HCl (pH 8.0)/150 mM glycine/20% (vol/vol) methanol. 3. Block membranes for 1.5 hours with 1X western wash buffer containing 5% non-fat dry milk (NFDM). 4. Incubate membranes for 1.5 hours at room temperature (RT) in NB 100-124 diluted 1:1,500 in 1X western wash/5% NFDM. 5. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes). 6. Incubate membranes with HRP conjugated anti-Mouse IgG for 1 hour (RT) in 1X western wash/5% NFDM. 7. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes). 8. Drain membrane and place on saran wrap. 9. Using Amersham ECL Kit, mix equal volumes of two reagents. Pour over membrane (protein side facing up). Let solution sit on membrane for 15-20 seconds. 10. Drain membrane and place on new saran wrap 11. Wrap up membrane and expose to film. 12. Develop accordingly. 10X Western wash 24.2g Tris 80g NaCl Tween-20 to 1% pH 7.6 and QS to 4L Stripping buffer 100 mM BME 2% SDS 62.5 mM Tris (pH 6.7) Incubate membrane for 30 minutes at 56 degrees C. Wash membrane 15 minutes with several changes of 1X western wash. Notes: If hypoxia treatment is not hypoxic enough (less than 2% oxygen to get an induction), signal will be absent. Also, if the harvest time is too slow or there are not enough protease inhibitors, etc., the induced protein will be rapidly lost as HIF-1beta has a very short half-life. Whole cell extracts or nuclear extracts of hypoxia induced cell lines (293, Hep3B, COS7, Hepa) are useful as a positive control. Nuclear Extract Preparation Reference: Wang and Semenza. Purification and Characterization of Hypoxia-Inducible Factor 1. Journal of Biological Chemistry. 270(3): 1230-1237, 1995.
  2. Do you provide any images of Human, WB citing the use of NB100-124?
    • Unfortunately, we do not have any images other than those listed on the website although at least 10 of the publications listed using this product mention using it for western blot.
  3. Can you please tell me the country of origin for antibody NB100-124 HIF-1 Beta [H1beta234] (ARNT) antibody?
    • It was produced in the USA.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-124AF405
Alexa Fluor 488 Labeled NB100-124AF488
Alexa Fluor 647 Labeled NB100-124AF647
Alexa Fluor 700 Labeled NB100-124AF700
Biotin Labeled NB100-124B
DyLight 350 Labeled NB100-124UV
DyLight 405 Labeled NB100-124V
DyLight 488 Labeled NB100-124G
DyLight 550 Labeled NB100-124R
DyLight 650 Labeled NB100-124C
DyLight 680 Labeled NB100-124FR
DyLight 755 Labeled NB100-124IR
FITC Labeled NB100-124F
HRP Labeled NB100-124H

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Blogs on ARNT/HIF-1 beta.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Lin Du
01/04/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol ARNT
Entrez