ARNT/HIF-1 beta Antibody (H1beta234)

Images

 
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Western Blot analysis with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124], theoretical molecular weight 86.6 kDa. Expression of CD44 mRNA in genetically ...read more
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably ...read more
Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Staining of human glioblastoma multi-forme utilizing ARNT/HIF-1 beta antibody (H1beta234) [NB100-124].
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Co-localization of HIF-2alpha with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124]. ICC/IF detecting indicated proteins using ...read more
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Analysis of HIF-1 beta in HeLa nuclear extract using ARNT/HIF-1 beta antibody (H1beta234) [NB100-124]. Theoretical molecular weight 86.6 kDa. Observed ...read more
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ma, Pm, ShSpecies Glossary
Applications WB, ChIP, GS, IB, ICC/IF, IHC, IHC-P, IP, CHIP-SEQ
Clone
H1beta234
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

ARNT/HIF-1 beta Antibody (H1beta234) Summary

Immunogen
ARNT/HIF-1 beta Antibody (H1beta234) was developed against a fusion protein containing amino acids 496-789 of human HIF-1 beta. [Uniprot: P27540]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ARNT
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation Sequencing
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Gel Super Shift Assays
  • Immunoblotting 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
In Western blot, a band at approximately 92 kDa is seen. Use in Gel Shift Super Assay reported in scientific literature (PMID: 11325839). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25343232)
Theoretical MW
86.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ARNT/HIF-1 beta Overexpression Lysate
HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
NIH 3T3 CoCl2 treated/Untreated Cell Lysate
L929 CoCl2 treated/Untreated Cell Lysate
Raw 264.7 CoCl2 treated/Untreated Cell Lysate
PC12 CoCl2 treated/Untreated Cell Lysate
PC12 Hypoxic / Normoxic Cell Lysate
Raw 264.7 Hypoxic / Normoxic Cell Lysate
L929 Hypoxic / Normoxic Cell Lysate
NIH 3T3 Hypoxic / Normoxic Cell Lysate
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB100-124 in the following applications:

Publications
Read Publications using
NB100-124 in the following applications:

  • 1 publication
  • 19 publications
  • GS
    1 publication
  • IB
    16 publications
  • 3 publications
  • IHC
    4 publications
  • 2 publications
  • IP
    6 publications
  • WB
    49 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for ARNT/HIF-1 beta Antibody (H1beta234)

  • ARNT protein
  • ARNT
  • aryl hydrocarbon receptor nuclear translocator
  • BHLHE2
  • Class E Basic Helix-Loop-Helix Protein 2
  • Dioxin Receptor, Nuclear Translocator
  • HIF1 beta
  • HIF-1 beta
  • HIF1B
  • HIF1BETA
  • HIF-1beta
  • HIF-1-beta
  • HIF1-beta
  • hypoxia-inducible factor 1, beta subunit
  • Hypoxia-inducible factor 1-beta
  • TANGO

Background

Aryl hydrocarbon nuclear translocator (ARNT), also commonly known as Hypoxia-inducible factor 1-beta (HIF-1 beta), is a ubiquitously expressed transcription factor that is part of the basic-helix-loop-helix (bHLH)-Per-ARNT-Sim (PAS) family (1, 2). Human arnt is located on chromosome 1q21 and encodes a protein 789 amino acids (aa) in length with a theoretical molecular weight of 87 kDa (1). Structurally, ARNT has a DNA binding bHLH domain, two PAS domains required for dimerization, and a transactivation domain/PAC region (1). ARNT belongs to the Class II bHLH-PAS proteins and is able to homodimerize or heterodimerize with the Class I proteins including AHA, AHRR, HIF-1 alpha, HIF-2 alpha, NPAS1, and SIM1 (2). Dimerization allows for efficient DNA binding and regulation of their target genes (2).

ARNT has an important role in two specific signaling pathways - the aryl hydrocarbon receptor (AhR) and the hypoxia inducible factor (HIF) pathway (1). In the AhR pathway, AhR in the cytosol is typically inactive and bound to heat shock protein 90 (hsp90) (3). Upon activation and ligand binding by environmental pollutants such as dioxins, AhR is translocated to the nucleus, dissociates from hsp90, and dimerizes with ARNT, leading to binding to response elements and expression of target genes including monooxygenases (1, 3). In the HIF pathway, under hypoxia (low oxygen) conditions prolylhydroxylase domain (PHD) enzymes and factor inhibiting HIF (FIH) are inhibited. HIF-1 alpha (or HIF-2 alpha) accumulates and is transported to the nucleus where it heterodimerizes with ARNT, allowing for binding to target gene's hypoxia response element (HRE), recruitment of coactivators, and transcription (1, 3). HIF-induced gene transcription plays a large role in tumor progression by promoting invasion, metastasis, de-differentiation and altered metabolism, and angiogenesis (1). While HIF-1 alpha's stability is dependent upon oxygen conditions, HIF-1 beta is stable in both normoxia and hypoxia (1-3).

The bHLH-PAS family and ARNT have been linked with a variety of pathologies and diseases including cancer, metabolic diseases, autoimmune diseases, and psychiatric disorders (2). ARNT/AHR is expressed in the skin and its pathway activation enhances skin barrier function and epidermal terminal differentiation, thus AHR agonists are currently being used as therapeutics for atopic dermatitis and psoriasis (4). Accordingly, studies of Arnt-deficient mice show profound abnormalities in skin barrier function and keratinization (4). Additionally, studies suggest that ARNT plays an important role in diabetes and beta-cell function (5). Islets from patients with type 2 diabetes have a significantly decreased ARNT expression compared to glucose-tolerant control donors (5). Modulation and stimulation of the HIF pathway may be a potential therapeutic strategy for treating type 2 diabetes and metabolic syndrome (5).

Alternate names for ARNT/HIF-1 beta include aryl hydrocarbon receptor nuclear translocator, BHLHE2, class E basic helix-loop-helix protein 2, Dixon receptor nuclear translocator, Hypoxia-inducible factor 1-beta, nuclear translocator, and TANGO.

References

1. Mandl, M., & Depping, R. (2014). Hypoxia-inducible aryl hydrocarbon receptor nuclear translocator (ARNT) (HIF-1beta): is it a rare exception?. Molecular medicine (Cambridge, Mass.). https://doi.org/10.2119/molmed.2014.00032

2. Wu, D., & Rastinejad, F. (2017). Structural characterization of mammalian bHLH-PAS transcription factors. Current opinion in structural biology. https://doi.org/10.1016/j.sbi.2016.09.011

3. Esser, C., & Rannug, A. (2015). The aryl hydrocarbon receptor in barrier organ physiology, immunology, and toxicology. Pharmacological reviews.https://doi.org/10.1124/pr.114.009001

4. Furue, M., Hashimoto-Hachiya, A., & Tsuji, G. (2019). Aryl Hydrocarbon Receptor in Atopic Dermatitis and Psoriasis. International journal of molecular sciences. https://doi.org/10.3390/ijms20215424

5. Girgis, C. M., Cheng, K., Scott, C. H., & Gunton, J. E. (2012). Novel links between HIFs, type 2 diabetes, and metabolic syndrome. Trends in endocrinology and metabolism: TEM, https://doi.org/10.1016/j.tem.2012.05.003

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Ma, Pm, Sh
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Publications for ARNT/HIF-1 beta Antibody (NB100-124)(99)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 9 applications: CHIP-SEQ, ChIP, GS, IB, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
CHIP-SEQ
(1)
ChIP
(19)
GS
(1)
IB
(16)
ICC/IF
(3)
IHC
(4)
IHC-P
(2)
IP
(6)
WB
(49)
All Applications
Filter By Species
Human
(70)
Mouse
(14)
Rat
(7)
All Species
Showing Publications 1 - 10 of 99. Show All 99 Publications.
Publications using NB100-124 Applications Species
Zhong, H et al. Hypoxia-inducible Factor 1 as a novel diagnostic therapeutic target in human Cancers. Recent Res. Devel. Cancer. 3: 49-68. 2001
Sasagawa T, Nagamatsu T, Yanagisawa M Et al. Hypoxia-inducible factor-1 beta is essential for upregulation of the hypoxia-induced FLT1 gene in placental trophoblasts Molecular human reproduction Oct 19 2021 [PMID: 34665260] (ICC/IF, Human) ICC/IF Human
Mehibel M, Xu Y, Li CG et al. Eliminating hypoxic tumor cells improves response to PARP inhibitors in homologous recombination-deficient cancer models The Journal of clinical investigation Jun 1 2021 [PMID: 34060485] (WB, Human) WB Human
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (ChIP, Mouse) ChIP Mouse
Tiwari A, Tashiro K, Dixit A Et Al. Loss of HIF1A From Pancreatic Cancer Cells Increases Expression of PPP1R1B and Degradation of p53 to Promote Invasion and Metastasis Gastroenterology Aug 3 2020 [PMID: 32768595] (Mouse) Mouse
D'Anna F, Van Dyck L, Xiong J et al. DNA methylation repels binding of hypoxia-inducible transcription factors to maintain tumor immunotolerance Genome Biol. Jul 27 2020 [PMID: 32718321] (CHIP-SEQ, Human, Mouse) CHIP-SEQ Human, Mouse
Olcina MM, Balanis NG, Kim RK et al. Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer Cell Rep Dec 26 2018 [PMID: 30590044] (WB, Human) WB Human
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (ChIP, Human) ChIP Human
Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca 2+ Release Stimulates Breast Cancer Stem Cell Enrichment Cell Rep. 2017 Feb 21 [PMID: 28228260] (ChIP, Human)

Details:
The positive impact of HIF inhibitors on breast cancer chemotherapy is explored through GSTO1 knockdown.
ChIP Human
Ju Julia A, Godet Ines, Ye I Chae et al. Hypoxia Selectively Enhances Integrin alpha 5 beta 1 Receptor Expression in Breast Cancer to Promote Metastasis. Molecular Cancer Research : Mcr 2017 Feb 17 [PMID: 28213554] (ChIP, Human) ChIP Human
Show All 99 Publications.

Reviews for ARNT/HIF-1 beta Antibody (NB100-124) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Other.

Reviews using NB100-124:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(3)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ARNT/HIF-1 beta NB100-124
Enlarge
5
reviewed by:
Verified Customer
WB Human 03/15/2021
View

Summary

ApplicationWestern Blot
Sample TestedHep3B human hepatocellular carcinoma cell line
SpeciesHuman
LotA-19
  5
reviewed by:
Verified Customer
WB Human 07/05/2019
View

Summary

ApplicationWestern Blot
Sample TestedHUMAN CANCER CELL LINES, WHOLE CELL LYSATES
SpeciesHuman
LotA-20
  4
reviewed by:
Verified Customer
WB Other 05/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedSH-SY5Y whole cell lysate
SpeciesOther
LotA14

Blocking

Blocking Details5% milk in PBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:1000 dilution in 1% BSA incubate at 4oC overnight

Secondary Antibody

Secondary DescriptionGoat anti mouse IgG, HRP
Secondary Manufacturer Cat#Millipore #AP308P
Secondary Concentration1:2000

Details

Detection NotesECL
  5
reviewed by:
Lin Du
WB Human 01/04/2011
View

Summary

ApplicationWestern Blot
Sample TestedT47D cell nuclear extract
SpeciesHuman

Product General Protocols

View specific protocols for ARNT/HIF-1 beta Antibody (NB100-124): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for ARNT/HIF-1 beta Antibody (NB100-124). (Showing 1 - 7 of 7 FAQs).

  1. Is this target appropriate for use in Chromatin Research?
    • Yes; here is a list of research areas that we have deemed appropriate for the target "ARNT/HIF-1 beta": Angiogenesis, Autophagy, Cancer, Cellular Markers, Chromatin Research, HIF Target Genes, Hypoxia, Transcription Factors and Regulators, Cardiovascular Biology, Lipid and Metabolism.
  2. For use in Western Blot with HIF-1 beta antibodies, what molecular weight of the band should I expect to see?
    • The theoretical molecular weight determined by our technical team for ARNT/HIF-1 beta antibodies is 86.6 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product, allowing the tested application/species to fall under our 100% guarantee. Please check out our Innovator's Reward Program if you decide to test a primary antibody with a species or application that is not currently listed. Please note that the Innovator's Reward Program only applies to our primary antibodies.
  4. Does your 100% guarantee apply to Western Blot for NB100-124?
    • Yes; use in Western Blot has been validated for NB100-124 through at least 50 publications found in the "Reviews & Publications" tab. If this product fails to work as stated, our technical service team will work with you to troubleshoot the issue. If this process is not successful, Novus will provide you with a free replacement or a 100% refund for the product. Guaranteed. For untested species and applications we offer our Innovator's program.
  5. Do you provide any images of Human, WB citing the use of NB100-124?
    • Unfortunately, we do not have any images other than those listed on the website although at least 10 of the publications listed using this product mention using it for western blot.
  6. Can you please tell me the country of origin for antibody NB100-124 HIF-1 Beta [H1beta234] (ARNT) antibody?
    • It was produced in the USA.
  7. Is NBP2-80582 the same as NB100-124?
    • NBP2-80582 is the Azide and BSA Free version of NB100-124. These products are the same except NBP2-80582 contains no preservative and NB100-124 contains 0.05% Sodium Azide as a preservative.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-124AF350
Alexa Fluor 405 NB100-124AF405
Alexa Fluor 488 NB100-124AF488
Alexa Fluor 532 NB100-124AF532
Alexa Fluor 594 NB100-124AF594
Alexa Fluor 647 NB100-124AF647
Alexa Fluor 700 NB100-124AF700
Alexa Fluor 750 NB100-124AF750
Biotin NB100-124B
DyLight 350 NB100-124UV
DyLight 405 NB100-124V
DyLight 488 NB100-124G
DyLight 550 NB100-124R
DyLight 594 NB100-124DL594
DyLight 650 NB100-124C
DyLight 680 NB100-124FR
DyLight 755 NB100-124IR
FITC NB100-124F
HRP NB100-124H
Janelia Fluor 549 NB100-124JF549
Janelia Fluor 646 NB100-124JF646

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Bioinformatics Tool for ARNT/HIF-1 beta Antibody (NB100-124)

Discover related pathways, diseases and genes to ARNT/HIF-1 beta Antibody (NB100-124). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for ARNT/HIF-1 beta Antibody (NB100-124)

Discover more about diseases related to ARNT/HIF-1 beta Antibody (NB100-124).
 

Pathways for ARNT/HIF-1 beta Antibody (NB100-124)

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PTMs for ARNT/HIF-1 beta Antibody (NB100-124)

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Blogs on ARNT/HIF-1 beta.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Verified Customer
03/15/2021
Application: WB
Species: Human

 
Verified Customer
07/05/2019
Application: WB
Species: Human

 
Verified Customer
05/28/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol ARNT