ARNT/HIF-1 beta Antibody (H1beta234)

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Western Blot analysis with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124], theoretical molecular weight 86.6 kDa. Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1A or HIF-2A expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1A-shRNA or HIF-2A-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. HIF-1B expression was used as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY licence.

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and ARNT/HIF-1 beta antibody (H1beta234)[NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight for ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY licence.

Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Staining of human glioblastoma multi-forme utilizing ARNT/HIF-1 beta antibody (H1beta234) [NB100-124].

Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Co-localization of HIF-2alpha with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124]. ICC/IF detecting indicated proteins using antibodies labelled with Alexa Fluor 555 (red, pseudocolour) and Alexa Fluor 488 (green, pseudocolour). 'Merge' is the red image superimposed onto the green image of the co-stained nuclear proteins. 'Coloc' is the co-localization channel calculated using ImageJ plugin. White indicates pixels where both red and green signal is found (i.e. co-localization). 'Overlay' is the co-localization image superimposed onto the merged image. Inlay is the magnified region (white square). White arrows highlight regions of co-localization. Scale bar, 5 um. Abbreviations: RNAPII, RNA Polymerase II phospho-serine 5. Image collected and cropped by CiteAb from the following publication (//rsob.royalsocietypublishing.org/lookup/doi/10.1098/rsob.160195), licensed under a CC-BY licence.

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Analysis of HIF-1 beta in HeLa nuclear extract using ARNT/HIF-1 beta antibody (H1beta234) [NB100-124]. Theoretical molecular weight 86.6 kDa. Observed molecular weight ~85 kDa.

Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124] at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu, Mu, Rt, Bv, Ma, Pm, Sh
• Applications: WB, ChIP, GS, IB, ICC/IF, IHC, IHC-P, IP, CHIP-SEQ
• Clone: H1beta234
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ARNT/HIF-1 beta Antibody (H1beta234) Summary

• Immunogen: ARNT/HIF-1 beta Antibody (H1beta234) was developed against a fusion protein containing amino acids 496-789 of human HIF-1 beta. [Uniprot: P27540]
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARNT
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation Sequencing
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Gel Super Shift Assays
  • Immunoblotting 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: In Western blot, a band at approximately 92 kDa is seen. Use in Gel Shift Super Assay reported in scientific literature (PMID: 11325839). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25343232)
• Theoretical MW: 86.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ARNT/HIF-1 beta Overexpression Lysate
  HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
  HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
  NIH 3T3 CoCl2 treated/Untreated Cell Lysate
  L929 CoCl2 treated/Untreated Cell Lysate
  Raw 264.7 CoCl2 treated/Untreated Cell Lysate
  PC12 CoCl2 treated/Untreated Cell Lysate
  PC12 Hypoxic / Normoxic Cell Lysate
  Raw 264.7 Hypoxic / Normoxic Cell Lysate
  L929 Hypoxic / Normoxic Cell Lysate
  NIH 3T3 Hypoxic / Normoxic Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ARNT/HIF-1 beta Antibody (H1beta234)

• ARNT protein
• ARNT
• aryl hydrocarbon receptor nuclear translocator
• BHLHE2
• Class E Basic Helix-Loop-Helix Protein 2
• Dioxin Receptor, Nuclear Translocator
• HIF1 beta
• HIF-1 beta
• HIF1B
• HIF1BETA
• HIF-1beta
• HIF-1-beta
• HIF1-beta
• hypoxia-inducible factor 1, beta subunit
• Hypoxia-inducible factor 1-beta
• TANGO

Background

Aryl hydrocarbon nuclear translocator (ARNT), also commonly known as Hypoxia-inducible factor 1-beta (HIF-1 beta), is a ubiquitously expressed transcription factor that is part of the basic-helix-loop-helix (bHLH)-Per-ARNT-Sim (PAS) family (1, 2). Human arnt is located on chromosome 1q21 and encodes a protein 789 amino acids (aa) in length with a theoretical molecular weight of 87 kDa (1). Structurally, ARNT has a DNA binding bHLH domain, two PAS domains required for dimerization, and a transactivation domain/PAC region (1). ARNT belongs to the Class II bHLH-PAS proteins and is able to homodimerize or heterodimerize with the Class I proteins including AHA, AHRR, HIF-1 alpha, HIF-2 alpha, NPAS1, and SIM1 (2). Dimerization allows for efficient DNA binding and regulation of their target genes (2).

ARNT has an important role in two specific signaling pathways - the aryl hydrocarbon receptor (AhR) and the hypoxia inducible factor (HIF) pathway (1). In the AhR pathway, AhR in the cytosol is typically inactive and bound to heat shock protein 90 (hsp90) (3). Upon activation and ligand binding by environmental pollutants such as dioxins, AhR is translocated to the nucleus, dissociates from hsp90, and dimerizes with ARNT, leading to binding to response elements and expression of target genes including monooxygenases (1, 3). In the HIF pathway, under hypoxia (low oxygen) conditions prolylhydroxylase domain (PHD) enzymes and factor inhibiting HIF (FIH) are inhibited. HIF-1 alpha (or HIF-2 alpha) accumulates and is transported to the nucleus where it heterodimerizes with ARNT, allowing for binding to target gene's hypoxia response element (HRE), recruitment of coactivators, and transcription (1, 3). HIF-induced gene transcription plays a large role in tumor progression by promoting invasion, metastasis, de-differentiation and altered metabolism, and angiogenesis (1). While HIF-1 alpha's stability is dependent upon oxygen conditions, HIF-1 beta is stable in both normoxia and hypoxia (1-3).

The bHLH-PAS family and ARNT have been linked with a variety of pathologies and diseases including cancer, metabolic diseases, autoimmune diseases, and psychiatric disorders (2). ARNT/AHR is expressed in the skin and its pathway activation enhances skin barrier function and epidermal terminal differentiation, thus AHR agonists are currently being used as therapeutics for atopic dermatitis and psoriasis (4). Accordingly, studies of Arnt-deficient mice show profound abnormalities in skin barrier function and keratinization (4). Additionally, studies suggest that ARNT plays an important role in diabetes and beta-cell function (5). Islets from patients with type 2 diabetes have a significantly decreased ARNT expression compared to glucose-tolerant control donors (5). Modulation and stimulation of the HIF pathway may be a potential therapeutic strategy for treating type 2 diabetes and metabolic syndrome (5).

Alternate names for ARNT/HIF-1 beta include aryl hydrocarbon receptor nuclear translocator, BHLHE2, class E basic helix-loop-helix protein 2, Dixon receptor nuclear translocator, Hypoxia-inducible factor 1-beta, nuclear translocator, and TANGO.

References

1. Mandl, M., & Depping, R. (2014). Hypoxia-inducible aryl hydrocarbon receptor nuclear translocator (ARNT) (HIF-1beta): is it a rare exception?. Molecular medicine (Cambridge, Mass.). https://doi.org/10.2119/molmed.2014.00032

2. Wu, D., & Rastinejad, F. (2017). Structural characterization of mammalian bHLH-PAS transcription factors. Current opinion in structural biology. https://doi.org/10.1016/j.sbi.2016.09.011

3. Esser, C., & Rannug, A. (2015). The aryl hydrocarbon receptor in barrier organ physiology, immunology, and toxicology. Pharmacological reviews.https://doi.org/10.1124/pr.114.009001

4. Furue, M., Hashimoto-Hachiya, A., & Tsuji, G. (2019). Aryl Hydrocarbon Receptor in Atopic Dermatitis and Psoriasis. International journal of molecular sciences. https://doi.org/10.3390/ijms20215424

5. Girgis, C. M., Cheng, K., Scott, C. H., & Gunton, J. E. (2012). Novel links between HIFs, type 2 diabetes, and metabolic syndrome. Trends in endocrinology and metabolism: TEM, https://doi.org/10.1016/j.tem.2012.05.003

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARNT/HIF-1 beta Antibody (NB100-124)(99)

Publications using NB100-124

• Zhong, H et al. Hypoxia-inducible Factor 1 as a novel diagnostic therapeutic target in human Cancers. Recent Res. Devel. Cancer. 3: 49-68. 2001
• Sasagawa T, Nagamatsu T, Yanagisawa M Et al. Hypoxia-inducible factor-1 beta is essential for upregulation of the hypoxia-induced FLT1 gene in placental trophoblasts Molecular human reproduction Oct 19 2021 [PMID: 34665260] (ICC/IF, Human)
• Mehibel M, Xu Y, Li CG et al. Eliminating hypoxic tumor cells improves response to PARP inhibitors in homologous recombination-deficient cancer models The Journal of clinical investigation Jun 1 2021 [PMID: 34060485] (WB, Human)
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (ChIP, Mouse)
• Tiwari A, Tashiro K, Dixit A Et Al. Loss of HIF1A From Pancreatic Cancer Cells Increases Expression of PPP1R1B and Degradation of p53 to Promote Invasion and Metastasis Gastroenterology Aug 3 2020 [PMID: 32768595] (Mouse)
• D'Anna F, Van Dyck L, Xiong J et al. DNA methylation repels binding of hypoxia-inducible transcription factors to maintain tumor immunotolerance Genome Biol. Jul 27 2020 [PMID: 32718321] (CHIP-SEQ, Human, Mouse)
• Olcina MM, Balanis NG, Kim RK et al. Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer Cell Rep Dec 26 2018 [PMID: 30590044] (WB, Human)
• Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (ChIP, Human)
• Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca 2+ Release Stimulates Breast Cancer Stem Cell Enrichment Cell Rep. 2017 Feb 21 [PMID: 28228260] (ChIP, Human)
  
  Details:
  The positive impact of HIF inhibitors on breast cancer chemotherapy is explored through GSTO1 knockdown.
• Ju Julia A, Godet Ines, Ye I Chae et al. Hypoxia Selectively Enhances Integrin alpha 5 beta 1 Receptor Expression in Breast Cancer to Promote Metastasis. Molecular Cancer Research : Mcr 2017 Feb 17 [PMID: 28213554] (ChIP, Human)
• Murakami A, Wang L, Kalhorn S et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma Oncogenesis Jan 16 2017 [PMID: 28092369] (WB, Human)
• Taylor SE, Bagnall J, Mason D et al. Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility Open Biol. 2016 Sep [PMID: 27655733] (ICC/IF, Human)
  
  Details:
  The differences in oxygen-dependent stability and gene activation between HIF-1 alpha and HIF-2 alpha are explored through fluorescent microscopy.
• Thienpont B, Steinbacher J, Zhao H et al. Tumour hypoxia causes DNA hypermethylation by reducing TET activity. Nature. Aug 17 2016 [PMID: 27533040] (ChIP, Mouse)
• Li S, Heller JJ, Bostick JW et al. Ikaros Inhibits Group 3 Innate Lymphoid Cell Development and Function by Suppressing the Aryl Hydrocarbon Receptor Pathway Immunity 2016 Jul 19 [PMID: 27438771] (WB, Human)
• Luo W, Chen I, Chen Y et al. PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia Oncotarget. 2016 Feb 9 [PMID: 26837221] (ChIP, Human)
  
  Details:
  The mechanism for feedback inhibition of hypoxia-inducible factors during prolonged hypoxia is clarified through studying the interaction of PRDX2 and PRDX4 with HIF-1 alpha and HIF-2 alpha.
• Altuntas S, Rossin F, Marsella C et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget 2015 Dec 29 [PMID: 26702927] (WB)
• Frey JL, Stonko DP, Faugere MC, Riddle RC Hypoxia-inducible factor-1alpha restricts the anabolic actions of parathyroid hormone. Bone Res 2014 May 13 [PMID: 26273518] (IP)
• Xiang L, Gilkes DM, Hu H et al. HIF-1alpha and TAZ serve as reciprocal co-activators in human breast cancer cells. Oncotarget. 2015 May 20 [PMID: 26059435] (IP, ChIP, WB, Human)
• Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014 [PMID: 25587023] (ChIP, Human)
• Kim KH, Kim D, Park JY et al. NNC 55-0396, a T-type Ca(2+) channel inhibitor, inhibits angiogenesis via suppression of hypoxia-inducible factor-1alpha signal transduction. J. Mol. Med. 2014 Dec 04 [PMID: 25471482] (WB, Human)
• Su Ej, Xin H, Yin P et al. Impaired fetoplacental angiogenesis in growth-restricted fetuses with abnormal umbilical artery Doppler velocimetry is mediated by aryl hydrocarbon receptor nuclear translocator (ARnT). J. Clin. Endocrinol. Metab. 2014 Oct 24 [PMID: 25343232] (WB, ICC/IF, IHC-P, Human)
• Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014 Oct 15 [PMID: 25326682] (ChIP, Human)
• Peng YJ, Yuan G, Khan S et al. Regulation of Hypoxia Inducible Factor-alpha Isoforms and Redox State By Carotid Body Neural Activity in Rats. J. Physiol. (Lond.). 2014 Jun 27 [PMID: 24973414] (WB, Rat)
• Wang T, Gilkes DM, Takano N et al. Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis. Proc Natl Acad Sci U S A. 2014 Jun 17 [PMID: 24938788] (ChIP, Human)
• Chaturvedi P, Gilkes DM, Takano N, Semenza GL. Hypoxia-inducible factor-dependent signaling between triple-negative breast cancer cells and mesenchymal stem cells promotes macrophage recruitment. Proc Natl Acad Sci U S A. 5/5/2014 [PMID: 24799675] (ChIP, Human)
• Zhang J, Ma WY. Nerve growth factor regulates the expression of vascular endothelial growth factor in human HaCaT keratinocytes via PI3K/mTOR pathway. Genet. Mol. Res. 1/24/2014 [PMID: 24615084] (WB, Human)
• D'Anna F, Van Dyck L, Xiong J et al. DNA Methylation Repels Binding of HIF Transcription Factors to Maintain Tumour Immunotolerance bioRxiv Jan 1 2020 (ChIP, Human)
• Rossin F, D'Eletto M, Falasca L et al. Transglutaminase 2 ablation leads to mitophagy impairment associated with a metabolic shift towards aerobic glycolysis. Cell Death Differ 2015 Mar [PMID: 25060553] (WB)
• Sena JA, Wang L, Pawlus MR. HIFs Enhance the Transcriptional Activation and Splicing of Adrenomedullin. Mol. Cancer Res. 2014 Feb 13 [PMID: 24523299] (WB, Human)
• Suzuki K, Nishi K, Takabuchi S et al. Differential roles of prostaglandin E-type receptors in activation of hypoxia-inducible factor 1 by prostaglandin E1 in vascular-derived cells under non-hypoxic conditions. PeerJ. 2013 Nov 28 [PMID: 24349900] (WB, Human)
• Takano N, Peng YJ, Kumar GK et al. Hypoxia-inducible factors regulate human and rat cystathionine B-synthase gene expression. Biochem J 2013 Dec 12 [PMID: 24328859] (ChIP, Human, Rat)
• Gilkes DM, Xiang L, Lee SJ et al. Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells. Proc Natl Acad Sci U S A 2014 Jan 21 [PMID: 24324133] (ChIP, Human)
• Xiang L, Gilkes DM, Chaturvedi P et al. Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer. J Mol Med (Berl). 2013 Nov 20 [PMID: 24248265] (WB, Human)
• Cai Z, Luo W, Zhan H, Semenza GL. Hypoxia-inducible factor 1 is required for remote ischemic preconditioning of the heart. Proc Natl Acad Sci U S A. 2013 Oct 22 [PMID: 24101519] (ChIP, WB, Mouse)
• Sena JA, Wang L, Hu CJ et al. BRG1 and BRM chromatin remodeling complexes regulate the hypoxia response by acting as a co-activator for a subset of HIF target genes. Mol Cell Biol 2013 Jul 29 [PMID: 23897427] (WB, ChIP, Human)
• Yuan G, Peng YJ, Reddy VD et al. Mutual antagonism between hypoxia-inducible factors 1alpha and 2alpha regulates oxygen sensing and cardio-respiratory homeostasis. Proc Natl Acad Sci U S A 2013 May 7 [PMID: 23610397] (WB, Human)
• Pawlus MR, Wang L, Hu CJ. STAT3 and HIF1alpha cooperatively activate HIF1 target genes in MDA-MB-231 and RCC4 cells. Oncogene 2013 Apr 22 [PMID: 23604114] (WB, Human)
• Balamurugan K, Sharan S, Klarmann KD et al. FBXW7alpha attenuates inflammatory signalling by downregulating C/EBPdelta and its target gene Tlr4. Nat Commun 2013 Apr 9 [PMID: 23575666] (WB, Mouse)
• Storti P, Bolzoni M, Donofrio G et al. Hypoxia-inducible factor (HIF)-1alpha suppression in myeloma cells blocks tumoral growth in vivo inhibiting angiogenesis and bone destruction. Leukemia 2013 Jan 24 [PMID: 23344526] (WB, Human)
• Chaturvedi P, Gilkes DM, Wong CC et al. Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis. J Clin Invest. 2013 Jan 2 [PMID: 23318994] (ChIP, Human)
• Luo W, Chang R, Zhong J et al. Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression. Proc Natl Acad Sci 2012 Nov 5 [PMID: 23129632] (ChIP, Human)
• Autheman D, Sheldon RA, Chaudhuri N et al. Glutathione peroxidase overexpression causes aberrant ERK activation in neonatal mouse cortex after hypoxic preconditioning. Pediatr Res 2012 Dec [PMID: 23007029] (Mouse)
• Pawlus MR, Wang L, Ware K, Hu CJ. USF2 and HIF2alpha cooperatively activate HIF2 target genes during hypoxia Mol Cell Biol 2012 Sep 10 [PMID: 22966206] (WB, Human)
• Krishnamachary B, Penet MF, Nimmagadda S et al. Hypoxia Regulates CD44 and Its Variant Isoforms through HIF-1alpha in Triple Negative Breast Cancer PLoS One 2012 [PMID: 22937154] (WB, Human)
• Chiabrando D, Fiorito V, Marro S et al. Cell-specific regulation of Ferroportin transcription following experimentally-induced acute anemia in mice Blood Cells Mol Dis 2012 Aug 23 [PMID: 22921471] (WB, IP, Human)
• Zhang D, Li J, Zhang M et al. The Requirement of c-Jun N-terminal kinase2 in Regulation of hypoxia inducing factor-1alpha mRNA Stability J Biol Chem 2012 Aug 21 [PMID: 22910906] (WB, Mouse)
• Singh AK, Mukhopadhyay C, Biswas S et al. Intracellular Pathogen Leishmania donovani Activates Hypoxia Inducible Factor-1 by Dual Mechanism for Survival Advantage within Macrophage PLoS One 2012 [PMID: 22701652] (WB, Mouse)
• Uniacke J, Holterman C, Lachance G, Franovic A, Jacob M, Fabian M, Payette J, Holcik M, Pause A, Lee S. An oxygen-regulated switch in the protein synthesis machinery. Nature doi:10.1038/nature11055. 2012 May 6. [PMID: 22678294] (WB, Human)
• Kaira K, Nakagawa K, Ohde Y, Okumura T, Takahashi T, Murakami H, Endo M, Kondo H, Nakajima T, Yamamoto N. Depolarized MUC1 Expression Is Closely Associated With Hypoxic Markers and Poor Outcome in Resected Non-Small Cell Lung Cancer. Int J Surg Pathol. 2011 Nov 21. [PMID: 22108499] (IHC, Human)
• Zhou L, Yang H. The von Hippel-Lindau Tumor Suppressor Protein Promotes c-Cbl-Independent Poly-Ubiquitylation and Degradation of the Activated EGFR. PLoS ONE;6(9). 2011 [PMID: 21949687] (WB, Human)
• Wong CC, Gilkes DM, Zhang H et al. Hypoxia-inducible factor 1 is a master regulator of breast cancer metastatic niche formation. Proc Natl Acad Sci U S A. Sep. 2011 [PMID: 21911388] (WB, Human)
• Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL. Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1. Cell;145(5):732-44. 2011 May 27. [PMID: 21620138] (ChIP, Human)
• Lin Q, Cong X, Yun Z. Differential hypoxic regulation of hypoxia-inducible factors 1alpha and 2alpha Mol Cancer Res 2011 Jun [PMID: 21571835] (WB, Human)
• Tamama K, Kawasaki H, Kerpedjieva SS et al. Differential roles of hypoxia inducible factor subunits in multipotential stromal cells under hypoxic condition. J Cell Biochem 2011 Mar [PMID: 21328454] (WB, IHC, Human)
• Peng YJ, Nanduri J, Khan SA et al. Hypoxia-inducible factor 2{alpha} (HIF-2{alpha}) heterozygous-null mice exhibit exaggerated carotid body sensitivity to hypoxia, breathing instability, and hypertension. Proc Natl Acad Sci U S A;108(7):3065-70. 2011 Feb 15. [PMID: 21288809] (WB, Mouse)
• Lunde IG, Anton SL, Bruusgaard JC et al. Hypoxia inducible factor 1 links fast-patterned muscle activity and fast muscle phenotype in rats. J Physiol;589(Pt 6):1443-54. 2011 Mar 15. [PMID: 21262877] (WB, Human, Rat)
• Terzuoli Erika, Puppo Maura, Rapisarda Annamaria et al. Aminoflavone, a ligand of the aryl hydrocarbon receptor, inhibits HIF-1alpha expression in an AhR-independent fashion. Cancer Res 2010 [PMID: 20736373] (WB, Mouse)
• Amir S, Wang R, Simons JW et al. SEPT9_v1 up-regulates hypoxia-inducible factor 1 by preventing its RACK1-mediated degradation. J Biol Chem. 284(17):11142-51. Epub 2009 Feb 26. 2009 Apr 24. [PMID: 19251694] (IB, Human)
• Kransler KM et al. Lung development in the Holtzman rat is adversely affected by gestational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Sci;107(2):498-511. 2009 Feb. [PMID: 18990726] (IHC-P, Rat)
• Lysiak JJ, Kirby JL, Tremblay JJ et al. Hypoxia-Inducible Factor-1{alpha} Is Constitutively Expressed in Murine Leydig Cells Regulates 3{beta}-Hydroxysteroid Dehydrogenase Type 1 Promoter Activity. J rol;30(2):146-156. 2009 [PMID: 18930903]
• Aminova, L, Siddiq, A, Ratan, R. Antioxidants, HIF prolyl hydroxylase inhibitors or short interfering RNAs to BNIP3 or PUMA, can prevent prodeath effects of the transcriptional activator, HIF-1alpha, in a mouse hippocampal neuronal line. Antioxid Redox Signal;10(12):1989-98. 2008 Dec. [PMID: 18774900] (IB, Mouse)
• Puppo M, Battaglia F, Ottaviano C et al. Topotecan inhibits vascular endothelial growth factor production and angiogenic activity induced by hypoxia in human neuroblastoma by targeting hypoxia-inducible factor-1alpha and -2alpha. Mol Cancer Ther;7(7):1974-84. 2008 Jul. [PMID: 18645007] (WB, Human)
• Lauzier M-C, Robitaille GA, Chan DA et al. (2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors. Mol Pharmacol;74(1):282-8. 2008 Jul. [PMID: 18424552] (WB, Human)
• Engebretsen, B et al. Acute hypobaric hypoxia (5486 m) induces greater pulmonary HIF-1 activation in hilltop compared to madison rats. High Alt Med Biol;8(4):312-21. 2007 Winter. [PMID: 18081507]
• Baek, JH et al. Spermidine/spermine N(1)-acetyltransferase-1 binds to hypoxia-inducible factor-1alpha (HIF-1alpha) and RACK1 and promotes ubiquitination and degradation of HIF-1alpha. J Biol Chem;282(46):33358-66. 2007 Nov 16. [PMID: 17875644] (IP, IB, Human)
• Cheng G et al. Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol;293(5):L1339-47. 2007 Nov. [PMID: 17873007]
• CJ Hu, A Sataur, L Wang et al. The N-terminal transactivation domain confers target gene specificity of hypoxia-inducible factors HIF-1a and HIF-2a. Mol Biol Cell. 2007 Nov [PMID: 17804822]
• Baek, JH et al. Spermidine/spermine-N1-acetyltransferase 2 is an essential component of the ubiquitin ligase complex that regulates hypoxia-inducible factor 1alpha. J Biol Chem;282(32):23572-80. 2007 Aug 10. [PMID: 17558023] (IP, IB, Human)
• Zhang, H et al. HIF-1 inhibits mitochondrial biogenesis and cellular respiration in VHL-deficient renal cell carcinoma by repression of C-MYC activity. Cancer Cell;11(5):407-20. 2007 May. [PMID: 17482131] (IB, Human)
• Shimojo N, Jesmin S, Zaedi S et al. Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy. Am J Physiol Heart Circ Physiol. 2007 July [PMID: 17369464] (WB, Rat)
• Asikainen TM, Chang LY, Coalson JJ et al. Improved lung growth function through hypoxia-inducible factor in primate chronic lung disease of prematurity. FASEB J;20(10):1698-700. 2006 Aug. [PMID: 16807366] (WB, Human)
• Slomiany, MG, Rosenzweig, SA. Hypoxia-inducible factor-1-dependent and -independent regulation of insulin-like growth factor-1-stimulated vascular endothelial growth factor secretion. J Pharmacol Exp Ther;318(2):666-75. 2006 Aug. [PMID: 16682453] (IB, Human)
• Nakamura, K et al. Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells. Cancer Res;66(8):4249-55. 2006 Apr 15. [PMID: 16618748] (IP, WB, Human)
• Hu, C-J et al. Differential regulation of the transcriptional activities of hypoxia-inducible factor 1 alpha (HIF-1alpha) and HIF-2alpha in stem cells. Mol Cell Biol;26(9):3514-26. 2006 May. [PMID: 16611993] (WB, Human)
• Okuyama, H et al. Expression of vascular endothelial growth factor receptor 1 in bone marrow-derived mesenchymal cells is dependent on hypoxia-inducible factor 1. J Biol Chem;281(22):15554-63. 2006 Jun 2. [PMID: 16574650] (IB, Human)
• Hui, AS et al. Calcium signaling stimulates translation of HIF-alpha during hypoxia. FASEB J;20(3):466-75. 2006 Mar. [PMID: 16507764] (WB, Rat)
• Oda, T et al. Activation of hypoxia-inducible factor 1 during macrophage differentiation. Am J Physiol Cell Physiol;291(1):C104-13. 2006 Jul. [PMID: 16481368]
• Amir, S et al. MSF-A interacts with hypoxia-inducible factor-1alpha and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis. Cancer Res;66(2):856-66. 2006 Jan 15. [PMID: 16424018] (IB, Human)
• Schultz, K et al. Hypoxia and hypoxia-inducible factor-1alpha promote growth factor-induced proliferation of human vascular smooth muscle cells. Am J Physiol Heart Circ Physiol;290(6):H2528-34. 2006 Jun. [PMID: 16399861] (WB, Human)
• Hirota, K et al. Induction of hypoxia-inducible factor 1 activity by muscarinic acetylcholine receptor signaling. J Biol Chem;279(40):41521-8. 2004 Oct 1. [PMID: 15280370] (IB , Human)
• Slomiany, M et al. IGF-1-induced VEGF and IGFBP-3 secretion correlates with increased HIF-1 alpha expression and activity in retinal pigment epithelial cell line D407. Invest Ophthalmol Vis Sci;45(8):2838-47. 2004 Aug. [PMID: 15277511] (IB, Human)
• Arsham, A et al. Akt and hypoxia-inducible factor-1 independently enhance tumor growth and angiogenesis. Cancer Res;64(10):3500-7. 2004 May 15. [PMID: 15150104] (WB, Mouse)
• Slomiany, M et al. Autocrine effects of IGF-I-induced VEGF and IGFBP-3 secretion in retinal pigment epithelial cell line ARPE-19. Am J Physiol Cell Physiol;287(3):C746-53. 2004 Sep. [PMID: 15140752] (IB, Human)
• Tissot van Patot, MC et al. Greater vascularity, lowered HIF-1/DNA binding, and elevated GSH as markers of adaptation to in vivo chronic hypoxia. Am J Physiol Lung Cell Mol Physiol;287(3):L525-32. 2004 Sep. [PMID: 15132953] (WB, Human)
• Rapisarda, A et al. Topoisomerase I-mediated inhibition of hypoxia-inducible factor 1: mechanism and therapeutic implications. Cancer Res;64(4):1475-82. 2004 Feb 15. [PMID: 14983893] (IB, Human)
• Alam, H et al. Follicle-stimulating hormone activation of hypoxia-inducible factor-1 by the phosphatidylinositol 3-kinase/AKT/Ras homolog enriched in brain (Rheb)/mammalian target of rapamycin (mTOR) pathway is necessary for induction of select protein markers of follicular differentiation. J Biol Chem;279(19):19431-40. 2004 May 7. [PMID: 14982927] (WB, Human)
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Reviews for ARNT/HIF-1 beta Antibody (NB100-124) (4)

Average Rating: 4.8

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Other.

reviewed by: Verified Customer WB Human 03/15/2021

Summary

• Application: Western Blot
• Sample Tested: Hep3B human hepatocellular carcinoma cell line
• Species: Human
• Lot: A-19

reviewed by: Verified Customer WB Human 07/05/2019

Summary

• Application: Western Blot
• Sample Tested: HUMAN CANCER CELL LINES, WHOLE CELL LYSATES
• Species: Human
• Lot: A-20

reviewed by: Verified Customer WB Other 05/28/2014

Summary

• Application: Western Blot
• Sample Tested: SH-SY5Y whole cell lysate
• Species: Other
• Lot: A14

Blocking

• Blocking Details: 5% milk in PBST for 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: 1:1000 dilution in 1% BSA incubate at 4oC overnight

Secondary Antibody

• Secondary Description: Goat anti mouse IgG, HRP
• Secondary Manufacturer Cat#: Millipore #AP308P
• Secondary Concentration: 1:2000

Details

• Detection Notes: ECL

reviewed by: Lin Du WB Human 01/04/2011

Summary

• Application: Western Blot
• Sample Tested: T47D cell nuclear extract
• Species: Human

FAQs for ARNT/HIF-1 beta Antibody (NB100-124). (Showing 1 - 7 of 7 FAQs).

1. Is this target appropriate for use in Chromatin Research?
   • Yes; here is a list of research areas that we have deemed appropriate for the target "ARNT/HIF-1 beta": Angiogenesis, Autophagy, Cancer, Cellular Markers, Chromatin Research, HIF Target Genes, Hypoxia, Transcription Factors and Regulators, Cardiovascular Biology, Lipid and Metabolism.
2. For use in Western Blot with HIF-1 beta antibodies, what molecular weight of the band should I expect to see?
   • The theoretical molecular weight determined by our technical team for ARNT/HIF-1 beta antibodies is 86.6 kDa.
3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product, allowing the tested application/species to fall under our 100% guarantee. Please check out our Innovator's Reward Program if you decide to test a primary antibody with a species or application that is not currently listed. Please note that the Innovator's Reward Program only applies to our primary antibodies.
4. Does your 100% guarantee apply to Western Blot for NB100-124?
   • Yes; use in Western Blot has been validated for NB100-124 through at least 50 publications found in the "Reviews & Publications" tab. If this product fails to work as stated, our technical service team will work with you to troubleshoot the issue. If this process is not successful, Novus will provide you with a free replacement or a 100% refund for the product. Guaranteed. For untested species and applications we offer our Innovator's program.
5. Do you provide any images of Human, WB citing the use of NB100-124?
   • Unfortunately, we do not have any images other than those listed on the website although at least 10 of the publications listed using this product mention using it for western blot.
6. Can you please tell me the country of origin for antibody NB100-124 HIF-1 Beta [H1beta234] (ARNT) antibody?
   • It was produced in the USA.
7. Is NBP2-80582 the same as NB100-124?
   • NBP2-80582 is the Azide and BSA Free version of NB100-124. These products are the same except NBP2-80582 contains no preservative and NB100-124 contains 0.05% Sodium Azide as a preservative.

Bioinformatics

• Gene Symbol: ARNT