ARNT/HIF-1 beta Antibody (H1beta234)

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Analysis of HIF-1 beta in HeLa nuclear extract.

Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HIF-1 beta (H1beta234) at 5ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Staining of human glioblastoma multi-forme utilizing anti-HIF-1 Beta.

• Reactivity: Hu, Mu, Rt, Bv, Ft, Pm, Sh
• Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP
• Clone: H1beta234
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.4 mg/ml

ARNT/HIF-1 beta Antibody (H1beta234) Summary

• Immunogen: Fusion protein containing amino acids 496-789 of human HIF-1 beta. [UniProt# P27540]
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARNT
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
• Application Notes: This HIF-1 beta (H1beta234) antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections and Chromatin Immunoprecipitation. By Western blot a band at approximately 92 kDa is seen. Use in Gel Shift Super Assay reported in scientific literature (PMID: 11325839).Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25343232) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  ARNT/HIF-1 beta Lysate (NBL1-07720)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.4 mg/ml
• Purity: Protein G purified

Alternate Names for ARNT/HIF-1 beta Antibody (H1beta234)

• ARNT protein
• ARNT
• aryl hydrocarbon receptor nuclear translocator
• BHLHE2
• HIF1 beta
• HIF-1 beta
• HIF1B
• HIF1BETA
• HIF-1-beta
• HIF1-beta
• hypoxia-inducible factor 1, beta subunit
• Hypoxia-inducible factor 1-beta
• nuclear translocator
• TANGO

Background

HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits which are bHLH proteins of PAS (PER, ARNT, SIM) family. HIF-1 beta forms heterodimer with AHR, AHRR, HIF1 alpha and EPAS1/HIF2 alpha as well as with other bHLH proteins and its dimerization is required for efficient DNA binding. It has been shown to interact with TACC3 and NOCA7 also. HIF-1 beta is also known as ARNT (AhR nuclear translocator) due to its ability to partner with AhR to form a heterodimeric transcription factor complex which is required for AhR activity. It is essential for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding and the AhR-HIF-1 beta complex then initiates transcription of genes involved in xenobiotic transformation, especially of PAHs. HIF-1 beta heterodimer with HIF1 alpha or EPAS1/HIF2 alpha functions as a transcriptional regulator of the adaptive response to hypoxia. Moreover, a chromosomal translocation resulting in TEL-ARNT fusion protein is associated with AML (acute myeloblastic leukemia) and decreased HIF-1 beta expression levels in pancreatic islets from NIDDM subjects reflects its important role in beta-cell function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARNT/HIF-1 beta Antibody (NB100-124)(90)

Publications using NB100-124

• Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (ChIP, Human)
• Luo W, Chen I, Chen Y et al. PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia. Oncotarget 2016 Feb 9 [PMID: 26837221] (WB)
• Zhong, H et al. Hypoxia-inducible Factor 1 as a novel diagnostic therapeutic target in human Cancers. Recent Res. Devel. Cancer. 3: 49-68. 2001
• Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca2+ Release Stimulates Breast Cancer Stem Cell Enrichment. Cell Rep. [PMID: 28228260] (WB, ChIP, Human)
• Murakami A, Wang L, Kalhorn S et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma Oncogenesis Jan 16 2017 [PMID: 28092369] (WB, Human)
• Taylor SE, Bagnall J, Mason D et al. Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility Open Biol Sep 1 2016 [PMID: 27655733] (ICC/IF, Human)
• Thienpont B, Steinbacher J, Zhao H et al. Tumour hypoxia causes DNA hypermethylation by reducing TET activity. Nature. Aug 17 2016 [PMID: 27533040] (ChIP, Mouse)
• Li S, Heller JJ, Bostick JW et al. Ikaros Inhibits Group 3 Innate Lymphoid Cell Development and Function by Suppressing the Aryl Hydrocarbon Receptor Pathway Immunity 2016 Jul 19 [PMID: 27438771] (WB, Human)
• Altuntas S, Rossin F, Marsella C et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget 2015 Dec 29 [PMID: 26702927] (WB)
• Frey JL, Stonko DP, Faugere MC, Riddle RC Hypoxia-inducible factor-1alpha restricts the anabolic actions of parathyroid hormone. Bone Res 2014 May 13 [PMID: 26273518] (IP)
• Xiang L, Gilkes DM, Hu H et al. HIF-1alpha and TAZ serve as reciprocal co-activators in human breast cancer cells. Oncotarget. 2015 May 20 [PMID: 26059435] (IP, ChIP, WB, Human)
• Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014 [PMID: 25587023] (ChIP, Human)
• Kim KH, Kim D, Park JY et al. NNC 55-0396, a T-type Ca(2+) channel inhibitor, inhibits angiogenesis via suppression of hypoxia-inducible factor-1alpha signal transduction. J. Mol. Med. 2014 Dec 04 [PMID: 25471482] (WB, Human)
• Su Ej, Xin H, Yin P et al. Impaired fetoplacental angiogenesis in growth-restricted fetuses with abnormal umbilical artery Doppler velocimetry is mediated by aryl hydrocarbon receptor nuclear translocator (ARnT). J. Clin. Endocrinol. Metab. 2014 Oct 24 [PMID: 25343232] (WB, ICC/IF, IHC-P, Human)
• Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014 Oct 15 [PMID: 25326682] (ChIP, Human)
• Rossin F, D'Eletto M, Falasca L et al. Transglutaminase 2 ablation leads to mitophagy impairment associated with a metabolic shift towards aerobic glycolysis. Cell Death Differ 2015 Mar [PMID: 25060553] (WB)
• Peng YJ, Yuan G, Khan S et al. Regulation of Hypoxia Inducible Factor-alpha Isoforms and Redox State By Carotid Body Neural Activity in Rats. J. Physiol. (Lond.). 2014 Jun 27 [PMID: 24973414] (WB, Rat)
• Wang T, Gilkes DM, Takano N et al. Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis. Proc Natl Acad Sci U S A. 2014 Jun 17 [PMID: 24938788] (ChIP, Human)
• Chaturvedi P, Gilkes DM, Takano N, Semenza GL. Hypoxia-inducible factor-dependent signaling between triple-negative breast cancer cells and mesenchymal stem cells promotes macrophage recruitment. Proc Natl Acad Sci U S A. 5/5/2014 [PMID: 24799675] (ChIP, Human)
• Zhang J, Ma WY. Nerve growth factor regulates the expression of vascular endothelial growth factor in human HaCaT keratinocytes via PI3K/mTOR pathway. Genet. Mol. Res. 1/24/2014 [PMID: 24615084] (WB, Human)
• Sena JA, Wang L, Pawlus MR. HIFs Enhance the Transcriptional Activation and Splicing of Adrenomedullin. Mol. Cancer Res. 2014 Feb 13 [PMID: 24523299] (WB, Human)
• Suzuki K, Nishi K, Takabuchi S et al. Differential roles of prostaglandin E-type receptors in activation of hypoxia-inducible factor 1 by prostaglandin E1 in vascular-derived cells under non-hypoxic conditions. PeerJ. 2013 Nov 28 [PMID: 24349900] (WB, Human)
• Takano N, Peng YJ, Kumar GK et al. Hypoxia-inducible factors regulate human and rat cystathionine B-synthase gene expression. Biochem J 2013 Dec 12 [PMID: 24328859] (ChIP, Human, Rat)
• Gilkes DM, Xiang L, Lee SJ et al. Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells. Proc Natl Acad Sci U S A 2014 Jan 21 [PMID: 24324133] (ChIP, Human)
• Xiang L, Gilkes DM, Chaturvedi P et al. Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer. J Mol Med (Berl). 2013 Nov 20 [PMID: 24248265] (WB, Human)
• Cai Z, Luo W, Zhan H, Semenza GL. Hypoxia-inducible factor 1 is required for remote ischemic preconditioning of the heart. Proc Natl Acad Sci U S A. 2013 Oct 22 [PMID: 24101519] (ChIP, WB, Mouse)
• Sena JA, Wang L, Hu CJ et al. BRG1 and BRM chromatin remodeling complexes regulate the hypoxia response by acting as a co-activator for a subset of HIF target genes. Mol Cell Biol 2013 Jul 29 [PMID: 23897427] (WB, ChIP, Human)
• Yuan G, Peng YJ, Reddy VD et al. Mutual antagonism between hypoxia-inducible factors 1alpha and 2alpha regulates oxygen sensing and cardio-respiratory homeostasis. Proc Natl Acad Sci U S A 2013 May 7 [PMID: 23610397] (WB, Human)
• Pawlus MR, Wang L, Hu CJ. STAT3 and HIF1alpha cooperatively activate HIF1 target genes in MDA-MB-231 and RCC4 cells. Oncogene 2013 Apr 22 [PMID: 23604114] (WB, Human)
• Balamurugan K, Sharan S, Klarmann KD et al. FBXW7alpha attenuates inflammatory signalling by downregulating C/EBPdelta and its target gene Tlr4. Nat Commun 2013 Apr 9 [PMID: 23575666] (WB, Mouse)
• Storti P, Bolzoni M, Donofrio G et al. Hypoxia-inducible factor (HIF)-1alpha suppression in myeloma cells blocks tumoral growth in vivo inhibiting angiogenesis and bone destruction. Leukemia 2013 Jan 24 [PMID: 23344526] (WB, Human)
• Chaturvedi P, Gilkes DM, Wong CC et al. Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis. J Clin Invest. 2013 Jan 2 [PMID: 23318994] (ChIP, Human)
• Luo W, Chang R, Zhong J et al. Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression. Proc Natl Acad Sci 2012 Nov 5 [PMID: 23129632] (ChIP, Human)
• Autheman D, Sheldon RA, Chaudhuri N et al. Glutathione peroxidase overexpression causes aberrant ERK activation in neonatal mouse cortex after hypoxic preconditioning. Pediatr Res 2012 Dec [PMID: 23007029] (Mouse)
• Pawlus MR, Wang L, Ware K, Hu CJ. USF2 and HIF2alpha cooperatively activate HIF2 target genes during hypoxia Mol Cell Biol 2012 Sep 10 [PMID: 22966206] (WB, Human)
• Krishnamachary B, Penet MF, Nimmagadda S et al. Hypoxia Regulates CD44 and Its Variant Isoforms through HIF-1alpha in Triple Negative Breast Cancer PLoS One 2012 [PMID: 22937154] (WB, Human)
• Chiabrando D, Fiorito V, Marro S et al. Cell-specific regulation of Ferroportin transcription following experimentally-induced acute anemia in mice Blood Cells Mol Dis 2012 Aug 23 [PMID: 22921471] (WB, IP, Human)
• Zhang D, Li J, Zhang M et al. The Requirement of c-Jun N-terminal kinase2 in Regulation of hypoxia inducing factor-1alpha mRNA Stability J Biol Chem 2012 Aug 21 [PMID: 22910906] (WB, Mouse)
• Singh AK, Mukhopadhyay C, Biswas S et al. Intracellular Pathogen Leishmania donovani Activates Hypoxia Inducible Factor-1 by Dual Mechanism for Survival Advantage within Macrophage PLoS One 2012 [PMID: 22701652] (WB, Mouse)
• Uniacke J, Holterman C, Lachance G, Franovic A, Jacob M, Fabian M, Payette J, Holcik M, Pause A, Lee S. An oxygen-regulated switch in the protein synthesis machinery. Nature doi:10.1038/nature11055. 2012 May 6. [PMID: 22678294] (WB, Human)
• Kaira K, Nakagawa K, Ohde Y, Okumura T, Takahashi T, Murakami H, Endo M, Kondo H, Nakajima T, Yamamoto N. Depolarized MUC1 Expression Is Closely Associated With Hypoxic Markers and Poor Outcome in Resected Non-Small Cell Lung Cancer. Int J Surg Pathol. 2011 Nov 21. [PMID: 22108499] (IHC, Human)
• Zhou L, Yang H. The von Hippel-Lindau Tumor Suppressor Protein Promotes c-Cbl-Independent Poly-Ubiquitylation and Degradation of the Activated EGFR. PLoS ONE;6(9). 2011 [PMID: 21949687] (WB, Human)
• Wong CC, Gilkes DM, Zhang H et al. Hypoxia-inducible factor 1 is a master regulator of breast cancer metastatic niche formation. Proc Natl Acad Sci U S A. Sep. 2011 [PMID: 21911388] (WB, Human)
• Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL. Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1. Cell;145(5):732-44. 2011 May 27. [PMID: 21620138] (ChIP, Human)
• Lin Q, Cong X, Yun Z. Differential hypoxic regulation of hypoxia-inducible factors 1alpha and 2alpha Mol Cancer Res 2011 Jun [PMID: 21571835] (WB, Human)
• Tamama K, Kawasaki H, Kerpedjieva SS et al. Differential roles of hypoxia inducible factor subunits in multipotential stromal cells under hypoxic condition. J Cell Biochem 2011 Mar [PMID: 21328454] (WB, IHC, Human)
• Peng YJ, Nanduri J, Khan SA et al. Hypoxia-inducible factor 2{alpha} (HIF-2{alpha}) heterozygous-null mice exhibit exaggerated carotid body sensitivity to hypoxia, breathing instability, and hypertension. Proc Natl Acad Sci U S A;108(7):3065-70. 2011 Feb 15. [PMID: 21288809] (WB, Mouse)
• Lunde IG, Anton SL, Bruusgaard JC et al. Hypoxia inducible factor 1 links fast-patterned muscle activity and fast muscle phenotype in rats. J Physiol;589(Pt 6):1443-54. 2011 Mar 15. [PMID: 21262877] (WB, Human, Rat)
• Amir S, Wang R, Simons JW et al. SEPT9_v1 up-regulates hypoxia-inducible factor 1 by preventing its RACK1-mediated degradation. J Biol Chem. 284(17):11142-51. Epub 2009 Feb 26. 2009 Apr 24. [PMID: 19251694] (IB, Human)
• Kransler KM et al. Lung development in the Holtzman rat is adversely affected by gestational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Sci;107(2):498-511. 2009 Feb. [PMID: 18990726] (IHC-P, Rat)
• Lysiak JJ, Kirby JL, Tremblay JJ et al. Hypoxia-Inducible Factor-1{alpha} Is Constitutively Expressed in Murine Leydig Cells Regulates 3{beta}-Hydroxysteroid Dehydrogenase Type 1 Promoter Activity. J rol;30(2):146-156. 2009 [PMID: 18930903]
• Aminova, L, Siddiq, A, Ratan, R. Antioxidants, HIF prolyl hydroxylase inhibitors or short interfering RNAs to BNIP3 or PUMA, can prevent prodeath effects of the transcriptional activator, HIF-1alpha, in a mouse hippocampal neuronal line. Antioxid Redox Signal;10(12):1989-98. 2008 Dec. [PMID: 18774900] (IB, Mouse)
• Puppo M, Battaglia F, Ottaviano C et al. Topotecan inhibits vascular endothelial growth factor production and angiogenic activity induced by hypoxia in human neuroblastoma by targeting hypoxia-inducible factor-1alpha and -2alpha. Mol Cancer Ther;7(7):1974-84. 2008 Jul. [PMID: 18645007] (WB, Human)
• Lauzier M-C, Robitaille GA, Chan DA et al. (2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors. Mol Pharmacol;74(1):282-8. 2008 Jul. [PMID: 18424552] (WB, Human)
• Engebretsen, B et al. Acute hypobaric hypoxia (5486 m) induces greater pulmonary HIF-1 activation in hilltop compared to madison rats. High Alt Med Biol;8(4):312-21. 2007 Winter. [PMID: 18081507]
• Baek, JH et al. Spermidine/spermine N(1)-acetyltransferase-1 binds to hypoxia-inducible factor-1alpha (HIF-1alpha) and RACK1 and promotes ubiquitination and degradation of HIF-1alpha. J Biol Chem;282(46):33358-66. 2007 Nov 16. [PMID: 17875644] (IP, IB, Human)
• Cheng G et al. Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol;293(5):L1339-47. 2007 Nov. [PMID: 17873007]
• CJ Hu, A Sataur, L Wang et al. The N-terminal transactivation domain confers target gene specificity of hypoxia-inducible factors HIF-1a and HIF-2a. Mol Biol Cell. 2007 Nov [PMID: 17804822]
• Baek, JH et al. Spermidine/spermine-N1-acetyltransferase 2 is an essential component of the ubiquitin ligase complex that regulates hypoxia-inducible factor 1alpha. J Biol Chem;282(32):23572-80. 2007 Aug 10. [PMID: 17558023] (IP, IB, Human)
• Zhang, H et al. HIF-1 inhibits mitochondrial biogenesis and cellular respiration in VHL-deficient renal cell carcinoma by repression of C-MYC activity. Cancer Cell;11(5):407-20. 2007 May. [PMID: 17482131] (IB, Human)
• Shimojo N, Jesmin S, Zaedi S et al. Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy. Am J Physiol Heart Circ Physiol. 2007 July [PMID: 17369464] (WB, Rat)
• Asikainen TM, Chang LY, Coalson JJ et al. Improved lung growth function through hypoxia-inducible factor in primate chronic lung disease of prematurity. FASEB J;20(10):1698-700. 2006 Aug. [PMID: 16807366] (WB, Human)
• Slomiany, MG, Rosenzweig, SA. Hypoxia-inducible factor-1-dependent and -independent regulation of insulin-like growth factor-1-stimulated vascular endothelial growth factor secretion. J Pharmacol Exp Ther;318(2):666-75. 2006 Aug. [PMID: 16682453] (IB, Human)
• Nakamura, K et al. Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells. Cancer Res;66(8):4249-55. 2006 Apr 15. [PMID: 16618748] (IP, WB, Human)
• Hu, C-J et al. Differential regulation of the transcriptional activities of hypoxia-inducible factor 1 alpha (HIF-1alpha) and HIF-2alpha in stem cells. Mol Cell Biol;26(9):3514-26. 2006 May. [PMID: 16611993] (WB, Human)
• Okuyama, H et al. Expression of vascular endothelial growth factor receptor 1 in bone marrow-derived mesenchymal cells is dependent on hypoxia-inducible factor 1. J Biol Chem;281(22):15554-63. 2006 Jun 2. [PMID: 16574650] (IB, Human)
• Hui, AS et al. Calcium signaling stimulates translation of HIF-alpha during hypoxia. FASEB J;20(3):466-75. 2006 Mar. [PMID: 16507764] (WB, Rat)
• Oda, T et al. Activation of hypoxia-inducible factor 1 during macrophage differentiation. Am J Physiol Cell Physiol;291(1):C104-13. 2006 Jul. [PMID: 16481368]
• Amir, S et al. MSF-A interacts with hypoxia-inducible factor-1alpha and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis. Cancer Res;66(2):856-66. 2006 Jan 15. [PMID: 16424018] (IB, Human)
• Schultz, K et al. Hypoxia and hypoxia-inducible factor-1alpha promote growth factor-induced proliferation of human vascular smooth muscle cells. Am J Physiol Heart Circ Physiol;290(6):H2528-34. 2006 Jun. [PMID: 16399861] (WB, Human)
• Hirota, K et al. Induction of hypoxia-inducible factor 1 activity by muscarinic acetylcholine receptor signaling. J Biol Chem;279(40):41521-8. 2004 Oct 1. [PMID: 15280370] (IB, Human)
• Slomiany, M et al. IGF-1-induced VEGF and IGFBP-3 secretion correlates with increased HIF-1 alpha expression and activity in retinal pigment epithelial cell line D407. Invest Ophthalmol Vis Sci;45(8):2838-47. 2004 Aug. [PMID: 15277511] (IB, Human)
• Arsham, A et al. Akt and hypoxia-inducible factor-1 independently enhance tumor growth and angiogenesis. Cancer Res;64(10):3500-7. 2004 May 15. [PMID: 15150104] (WB, Mouse)
• Slomiany, M et al. Autocrine effects of IGF-I-induced VEGF and IGFBP-3 secretion in retinal pigment epithelial cell line ARPE-19. Am J Physiol Cell Physiol;287(3):C746-53. 2004 Sep. [PMID: 15140752] (IB, Human)
• Tissot van Patot, MC et al. Greater vascularity, lowered HIF-1/DNA binding, and elevated GSH as markers of adaptation to in vivo chronic hypoxia. Am J Physiol Lung Cell Mol Physiol;287(3):L525-32. 2004 Sep. [PMID: 15132953] (WB, Human)
• Rapisarda, A et al. Topoisomerase I-mediated inhibition of hypoxia-inducible factor 1: mechanism and therapeutic implications. Cancer Res;64(4):1475-82. 2004 Feb 15. [PMID: 14983893] (IB, Human)
• Alam, H et al. Follicle-stimulating hormone activation of hypoxia-inducible factor-1 by the phosphatidylinositol 3-kinase/AKT/Ras homolog enriched in brain (Rheb)/mammalian target of rapamycin (mTOR) pathway is necessary for induction of select protein markers of follicular differentiation. J Biol Chem;279(19):19431-40. 2004 May 7. [PMID: 14982927] (WB, Human)
• Hu CJ, Wang LY, Chodosh LA et al. Differential roles of hypoxia-inducible factor 1alpha (HIF-1alpha) and HIF-2alpha in hypoxic gene regulation. Mol Cell Biol 2003 Dec [PMID: 14645546]
• Fukuda, R et al. Vascular endothelial growth factor gene expression in colon cancer cells exposed to prostaglandin E2 is mediated by hypoxia-inducible factor 1. Cancer Res;63(9):2330-4. 2003 May 1. [PMID: 12727858] (IB, Human)
• Mabjeesh, N et al. 2ME2 inhibits tumor growth and angiogenesis by disrupting microtubules and dysregulating HIF. Cancer Cell;3(4):363-75. 2003 Apr. [PMID: 12726862] (IB, Human)
• Mabjeesh, N. Dibenzoylmethane, a natural dietary compound, induces HIF-1 alpha and increases expression of VEGF. Biochem Biophys Res Commun;303(1):279-86. 2003 Mar 28. [PMID: 12646199] (WB, Human)
• Elvert, G et al. Cooperative interaction of hypoxia-inducible factor-2alpha (HIF-2alpha ) and Ets-1 in the transcriptional activation of vascular endothelial growth factor receptor-2 (Flk-1). J Biol Chem;278(9):7520-30. 2003 Feb 28. [PMID: 12464608]
• Volm, M et al. Protein expression profiles indicative for drug resistance of non-small cell lung cancer. Br J Cancer;87(3):251-7. 2002 Jul 29. [PMID: 12177790] (IHC, Human)
• Rapisarda, A et al. Identification of small molecule inhibitors of hypoxia-inducible factor 1 transcriptional activation pathway. Cancer Res;62(15):4316-24. 2002 Aug 1. [PMID: 12154035] (WB, Human)
• Fukuda, R et al. Insulin-like growth factor 1 induces hypoxia-inducible factor 1-mediated vascular endothelial growth factor expression, which is dependent on MAP kinase and phosphatidylinositol 3-kinase signaling in colon cancer cells. J Biol Chem;277(41):38205-11. 2002 Oct 11. [PMID: 12149254] (IB, Human)
• Bernaudin, M et al. Brain genomic response following hypoxia and re-oxygenation in the neonatal rat. Identification of genes that might contribute to hypoxia-induced ischemic tolerance. J Biol Chem. 277(42):39728-38. 2002 Oct 18. [PMID: 12145288] (WB, Rat)
• Mabjeesh, N et al. Geldanamycin induces degradation of hypoxia-inducible factor 1alpha protein via the proteosome pathway in prostate cancer cells. Cancer Res;62(9):2478-82. 2002 May 1. [PMID: 11980636] (WB, Human)
• Zhong, H et al. Hypoxia-inducible factor 1alpha and 1beta proteins share common signaling pathways in human prostate cancer cells. Biochem Biophys Res Commun;284(2):352-6. 2001 Jun 8. [PMID: 11394885] (IB, Human)
• Tendler, D et al. Intersection of interferon and hypoxia signal transduction pathways in nitric oxide-induced tumor apoptosis. Cancer Res;61(9):3682-8. 2001 May 1. [PMID: 11325839] (GS, Mouse)
• Zagzag, D et al. Expression of hypoxia-inducible factor 1alpha in brain tumors: association with angiogenesis, invasion, and progression. Cancer;88(11):2606-18. 2000 Jun 1. [PMID: 10861440] (WB, IHC, Human)

Review for ARNT/HIF-1 beta Antibody (NB100-124) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Lin Du WB Human 01/04/2011

Summary

• Application: Western Blot
• Sample Tested: T47D cell nuclear extract
• Species: Human

FAQs for ARNT/HIF-1 beta Antibody (NB100-124). (Showing 1 - 3 of 3 FAQs).

1. Does your guarantee to apply WB of NB100-124?
   • For NB100-124, we have the following Western Blot Procedure: 1. Resolve aliquots (15 mg) of induced nuclear protein extracts on a SDS/6% polyacrylamide gel. 2. Transfer to nitrocellulose membranes in 20 mM Tris-HCl (pH 8.0)/150 mM glycine/20% (vol/vol) methanol. 3. Block membranes for 1.5 hours with 1X western wash buffer containing 5% non-fat dry milk (NFDM). 4. Incubate membranes for 1.5 hours at room temperature (RT) in NB 100-124 diluted 1:1,500 in 1X western wash/5% NFDM. 5. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes). 6. Incubate membranes with HRP conjugated anti-Mouse IgG for 1 hour (RT) in 1X western wash/5% NFDM. 7. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes). 8. Drain membrane and place on saran wrap. 9. Using Amersham ECL Kit, mix equal volumes of two reagents. Pour over membrane (protein side facing up). Let solution sit on membrane for 15-20 seconds. 10. Drain membrane and place on new saran wrap 11. Wrap up membrane and expose to film. 12. Develop accordingly. 10X Western wash 24.2g Tris 80g NaCl Tween-20 to 1% pH 7.6 and QS to 4L Stripping buffer 100 mM BME 2% SDS 62.5 mM Tris (pH 6.7) Incubate membrane for 30 minutes at 56 degrees C. Wash membrane 15 minutes with several changes of 1X western wash. Notes: If hypoxia treatment is not hypoxic enough (less than 2% oxygen to get an induction), signal will be absent. Also, if the harvest time is too slow or there are not enough protease inhibitors, etc., the induced protein will be rapidly lost as HIF-1beta has a very short half-life. Whole cell extracts or nuclear extracts of hypoxia induced cell lines (293, Hep3B, COS7, Hepa) are useful as a positive control. Nuclear Extract Preparation Reference: Wang and Semenza. Purification and Characterization of Hypoxia-Inducible Factor 1. Journal of Biological Chemistry. 270(3): 1230-1237, 1995.
2. Do you provide any images of Human, WB citing the use of NB100-124?
   • Unfortunately, we do not have any images other than those listed on the website although at least 10 of the publications listed using this product mention using it for western blot.
3. Can you please tell me the country of origin for antibody NB100-124 HIF-1 Beta [H1beta234] (ARNT) antibody?
   • It was produced in the USA.

Bioinformatics

• Gene Symbol: ARNT
• Entrez:
  • 405(Human)
  • 11863(Mouse)
  • 25242(Rat)