ARNT/HIF-1 beta Antibody (H1beta234)


Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1A or HIF-2A expression in whole cell extracts from more
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably more
Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Staining of human glioblastoma multi-forme utilizing anti-HIF-1 Beta.
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Co-localization of HIF-2alpha with HIF-1beta. Immunofluorescence detecting indicated proteins using antibodies labelled with more
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Analysis of HIF-1 beta in HeLa nuclear extract.
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. more

Product Details

Reactivity Hu, Mu, Rt, Bv, Ma, Pm, ShSpecies Glossary
Applications WB, ChIP, GS, IB, ICC/IF, IHC, IHC-P, IP
1 mg/ml

ARNT/HIF-1 beta Antibody (H1beta234) Summary

Fusion protein containing amino acids 496-789 of human HIF-1 beta. [Uniprot: P27540]
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Gel Super Shift Assays
  • Immunoblotting 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
Application Notes
In Western blot, a band at approximately 92 kDa is seen. Use in Gel Shift Super Assay reported in scientific literature (PMID: 11325839). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25343232)
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
ARNT/HIF-1 beta Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
NB100-124 in the following applications:

Read Publications using
NB100-124 in the following applications:

  • 17 publications
  • GS
    1 publication
  • IB
    16 publications
  • 2 publications
  • IHC
    4 publications
  • 2 publications
  • IP
    6 publications
  • WB
    50 publications

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for ARNT/HIF-1 beta Antibody (H1beta234)

  • ARNT protein
  • ARNT
  • aryl hydrocarbon receptor nuclear translocator
  • BHLHE2
  • HIF1 beta
  • HIF-1 beta
  • HIF1B
  • HIF-1-beta
  • HIF1-beta
  • hypoxia-inducible factor 1, beta subunit
  • Hypoxia-inducible factor 1-beta
  • nuclear translocator


HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits which are bHLH proteins of PAS (PER, ARNT, SIM) family. HIF-1 beta forms heterodimer with AHR, AHRR, HIF1 alpha and EPAS1/HIF2 alpha as well as with other bHLH proteins and its dimerization is required for efficient DNA binding. It has been shown to interact with TACC3 and NOCA7 also. HIF-1 beta is also known as ARNT (AhR nuclear translocator) due to its ability to partner with AhR to form a heterodimeric transcription factor complex which is required for AhR activity. It is essential for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding and the AhR-HIF-1 beta complex then initiates transcription of genes involved in xenobiotic transformation, especially of PAHs. HIF-1 beta heterodimer with HIF1 alpha or EPAS1/HIF2 alpha functions as a transcriptional regulator of the adaptive response to hypoxia. Moreover, a chromosomal translocation resulting in TEL-ARNT fusion protein is associated with AML (acute myeloblastic leukemia) and decreased HIF-1 beta expression levels in pancreatic islets from NIDDM subjects reflects its important role in beta-cell function.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Publications for ARNT/HIF-1 beta Antibody (NB100-124)(94)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ChIP, GS, IB, ICC/IF, IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 94. Show All 94 Publications.
Publications using NB100-124 Applications Species
Olcina MM, Balanis NG, Kim RK et al. Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer Cell Rep Dec 26 2018 [PMID: 30590044] (WB, Human) WB Human
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (ChIP, Human) ChIP Human
Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca2+ Release Stimulates Breast Cancer Stem Cell Enrichment. Cell Rep. [PMID: 28228260] (WB, ChIP, Human) WB, ChIP Human
Ju Julia A, Godet Ines, Ye I Chae et al. Hypoxia Selectively Enhances Integrin alpha 5 beta 1 Receptor Expression in Breast Cancer to Promote Metastasis. Molecular Cancer Research : Mcr 2017 Feb 17 [PMID: 28213554] (ChIP, Human) ChIP Human
Murakami A, Wang L, Kalhorn S et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma Oncogenesis Jan 16 2017 [PMID: 28092369] (WB, Human) WB Human
Taylor SE, Bagnall J, Mason D et al. Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility Open Biol Sep 1 2016 [PMID: 27655733] (ICC/IF, Human) ICC/IF Human
Thienpont B, Steinbacher J, Zhao H et al. Tumour hypoxia causes DNA hypermethylation by reducing TET activity. Nature. Aug 17 2016 [PMID: 27533040] (ChIP, Mouse) ChIP Mouse
Li S, Heller JJ, Bostick JW et al. Ikaros Inhibits Group 3 Innate Lymphoid Cell Development and Function by Suppressing the Aryl Hydrocarbon Receptor Pathway Immunity 2016 Jul 19 [PMID: 27438771] (WB, Human) WB Human
Luo W, Chen I, Chen Y et al. PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia. Oncotarget 2016 Feb 9 [PMID: 26837221] (WB) WB
Altuntas S, Rossin F, Marsella C et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget 2015 Dec 29 [PMID: 26702927] (WB) WB
Show All 94 Publications.

Reviews for ARNT/HIF-1 beta Antibody (NB100-124) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-124:
Filter by Applications
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Filter by Species
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 07/05/2019


ApplicationWestern Blot
reviewed by:
Lin Du
WB Human 01/04/2011


ApplicationWestern Blot
Sample TestedT47D cell nuclear extract

Product General Protocols

View specific protocols for ARNT/HIF-1 beta Antibody (NB100-124): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for ARNT/HIF-1 beta Antibody (NB100-124). (Showing 1 - 3 of 3 FAQs).

  1. Does your guarantee to apply WB of NB100-124?
    • For NB100-124, we have the following Western Blot Procedure: 1. Resolve aliquots (15 mg) of induced nuclear protein extracts on a SDS/6% polyacrylamide gel. 2. Transfer to nitrocellulose membranes in 20 mM Tris-HCl (pH 8.0)/150 mM glycine/20% (vol/vol) methanol. 3. Block membranes for 1.5 hours with 1X western wash buffer containing 5% non-fat dry milk (NFDM). 4. Incubate membranes for 1.5 hours at room temperature (RT) in NB 100-124 diluted 1:1,500 in 1X western wash/5% NFDM. 5. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes). 6. Incubate membranes with HRP conjugated anti-Mouse IgG for 1 hour (RT) in 1X western wash/5% NFDM. 7. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes). 8. Drain membrane and place on saran wrap. 9. Using Amersham ECL Kit, mix equal volumes of two reagents. Pour over membrane (protein side facing up). Let solution sit on membrane for 15-20 seconds. 10. Drain membrane and place on new saran wrap 11. Wrap up membrane and expose to film. 12. Develop accordingly. 10X Western wash 24.2g Tris 80g NaCl Tween-20 to 1% pH 7.6 and QS to 4L Stripping buffer 100 mM BME 2% SDS 62.5 mM Tris (pH 6.7) Incubate membrane for 30 minutes at 56 degrees C. Wash membrane 15 minutes with several changes of 1X western wash. Notes: If hypoxia treatment is not hypoxic enough (less than 2% oxygen to get an induction), signal will be absent. Also, if the harvest time is too slow or there are not enough protease inhibitors, etc., the induced protein will be rapidly lost as HIF-1beta has a very short half-life. Whole cell extracts or nuclear extracts of hypoxia induced cell lines (293, Hep3B, COS7, Hepa) are useful as a positive control. Nuclear Extract Preparation Reference: Wang and Semenza. Purification and Characterization of Hypoxia-Inducible Factor 1. Journal of Biological Chemistry. 270(3): 1230-1237, 1995.
  2. Do you provide any images of Human, WB citing the use of NB100-124?
    • Unfortunately, we do not have any images other than those listed on the website although at least 10 of the publications listed using this product mention using it for western blot.
  3. Can you please tell me the country of origin for antibody NB100-124 HIF-1 Beta [H1beta234] (ARNT) antibody?
    • It was produced in the USA.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-124AF350
Alexa Fluor 405 NB100-124AF405
Alexa Fluor 488 NB100-124AF488
Alexa Fluor 532 NB100-124AF532
Alexa Fluor 594 NB100-124AF594
Alexa Fluor 647 NB100-124AF647
Alexa Fluor 700 NB100-124AF700
Alexa Fluor 750 NB100-124AF750
Biotin NB100-124B
DyLight 350 NB100-124UV
DyLight 405 NB100-124V
DyLight 488 NB100-124G
DyLight 550 NB100-124R
DyLight 594 NB100-124DL594
DyLight 650 NB100-124C
DyLight 680 NB100-124FR
DyLight 755 NB100-124IR
FITC NB100-124F
HRP NB100-124H
Janelia Fluor 549 NB100-124JF549
Janelia Fluor 646 NB100-124JF646

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Blogs on ARNT/HIF-1 beta.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

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Recent Reviews


Application: WB
Species: Human

Lin Du
Application: WB
Species: Human


Gene Symbol ARNT