Recombinant Human TNF-alpha Protein

Images

 
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA

Order Details

Recombinant Human TNF-alpha Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
The specific activity of Recombinant Human TNF-alpha is approximately 7.6 x 104 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786).
Source
E. coli-derived
Val77-Leu233, with and without an N-terminal Met
Accession #
N-terminal Sequence
Met & Val77
Protein/Peptide Type
Recombinant Proteins
Gene
TNF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
210-TA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF-alpha Protein

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).

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210-TA
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Applications: BA

Publications for (421)

We have publications tested in 15 confirmed species: Human, Mouse, Rat, Bovine, Canine, Feline, Guinea Pig, Hamster, N/A, Ovine, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 9 applications: Bioassay, ELISA Standard, In Vivo, Media Supplement - Cell Prep, Not Specified, Standard, Stimulation, WB Standard, cell culture.


Filter By Application
Bioassay
(388)
ELISA Standard
(14)
In Vivo
(2)
Media Supplement - Cell Prep
(1)
Not Specified
(2)
Standard
(1)
Stimulation
(1)
WB Standard
(1)
cell culture
(2)
All Applications
Filter By Species
Human
(369)
Mouse
(8)
Rat
(2)
Bovine
(5)
Canine
(2)
Feline
(1)
Guinea Pig
(1)
Hamster
(1)
N/A
(14)
Ovine
(1)
Porcine
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(4)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 421. Show All 421 Publications.
Publications using 210-TA Applications Species
L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis Autophagy, 2017;0(0):0. 2017 [PMID: 28933598] (Bioassay, Human) Bioassay Human
N Starc, D Ingo, A Conforti, V Rossella, L Tomao, A Pitisci, F De Mattia, I Brigida, M Algeri, M Montanari, G Palumbo, P Merli, P Rossi, A Aiuti, F Locatelli, ME Bernardo Biological and functional characterization of bone marrow-derived mesenchymal stromal cells from patients affected by primary immunodeficiency Sci Rep, 2017;7(1):8153. 2017 [PMID: 28811575] (Bioassay, Human) Bioassay Human
KA Bailey, FG Haj, SI Simon, AG Passerini Atherosusceptible Shear Stress Activates Endoplasmic Reticulum Stress to Promote Endothelial Inflammation Sci Rep, 2017;7(1):8196. 2017 [PMID: 28811527] (Bioassay, Human) Bioassay Human
AE van der Wi, IMC Vogels, CJF van Noorde, I Klaassen, RO Schlingema TNF?-Induced Disruption of the Blood-Retinal Barrier In Vitro Is Regulated by Intracellular 3&#039;,5&#039;-Cyclic Adenosine Monophosphate Levels Invest. Ophthalmol. Vis. Sci., 2017;58(9):3496-3505. 2017 [PMID: 28715583] (Bioassay, Human) Bioassay Human
E Couderc, F Morel, P Levillain, A Buffière-M, M Camus, C Paquier, C Bodet, JF Jégou, M Pohin, L Favot, M Garcia, V Huguier, J Mcheik, C Lacombe, H Yssel, G Guillet, FX Bernard, JC Lecron Interleukin-17A-induced production of acute serum amyloid A by keratinocytes contributes to psoriasis pathogenesis PLoS ONE, 2017;12(7):e0181486. 2017 [PMID: 28708859] (Bioassay, Human) Bioassay Human
R Ahmad, B Rah, D Bastola, P Dhawan, AB Singh Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching Sci Rep, 2017;7(1):5125. 2017 [PMID: 28698546] (Bioassay, Human) Bioassay Human
J Yuzugulen, JA Douthwaite, EG Wood, IC Villar, NSA Patel, J Jegard, H Gaertner, I Rossitto-B, K Rose, O Hartley, PR Cutillas, A Ahluwalia, R Corder Characterisation of preproendothelin-1 derived peptides identifies Endothelin-Like Domain Peptide as a modulator of Endothelin-1 Sci Rep, 2017;7(1):4956. 2017 [PMID: 28694457] (Bioassay, Human) Bioassay Human
SS Chung, Y Wu, Q Okobi, D Adekoya, M Atefi, O Clarke, P Dutta, JV Vadgama Proinflammatory Cytokines IL-6 and TNF-? Increased Telomerase Activity through NF-?B/STAT1/STAT3 Activation, and Withaferin A Inhibited the Signaling in Colorectal Cancer Cells Mediators Inflamm., 2017;2017(0):5958429. 2017 [PMID: 28676732] (Bioassay, Human) Bioassay Human
R Connor, LD Jones, X Qiu, J Thakar, SB Maggirwar Frontline Science: c-Myc regulates P-selectin glycoprotein ligand-1 expression in monocytes during HIV-1 infection J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28663244] (Bioassay, Human) Bioassay Human
L Šenolt, K Prajzlerov, H Hulejová, B Šumová, M Filková, D Veigl, K Pavelka, J Vencovský Interleukin-20 is triggered by TLR ligands and associates with disease activity in patients with rheumatoid arthritis Cytokine, 2017;97(0):187-192. 2017 [PMID: 28662439] (Bioassay, Human) Bioassay Human
Show All 421 Publications.

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FAQs for . (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Bioinformatics

Gene Symbol TNF
Entrez
Uniprot