Recombinant Human TNF-alpha Protein

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Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TNF-alpha Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
The specific activity of Recombinant Human TNF-alpha is approximately 7.6 x 104 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786).
Source
E. coli-derived human TNF-alpha protein
Val77-Leu233, with and without an N-terminal Met
Accession #
N-terminal Sequence
Met & Val77
Protein/Peptide Type
Recombinant Proteins
Gene
TNF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 5
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210-TA in the following application:

Publications
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210-TA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 0.1-1 mg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF-alpha Protein

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Pennica, D. et al. (1984) Nature 312:724.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

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Publications for TNF-alpha (210-TA)(526)

We have publications tested in 15 confirmed species: Human, Mouse, Rat, Bovine, Canine, Feline, Guinea Pig, Hamster, N/A, Ovine, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 10 applications: Bioassay, ELISA Standard, Hemofiltration Procedure, In Vivo, Media Supplement - Cell Prep, Not Specified, Standard, Stimulation, WB Standard, cell culture.


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Bioassay
(492)
ELISA Standard
(14)
Hemofiltration Procedure
(1)
In Vivo
(2)
Media Supplement - Cell Prep
(1)
Not Specified
(2)
Standard
(1)
Stimulation
(1)
WB Standard
(1)
cell culture
(2)
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Human
(465)
Mouse
(13)
Rat
(3)
Bovine
(5)
Canine
(2)
Feline
(1)
Guinea Pig
(1)
Hamster
(1)
N/A
(14)
Ovine
(1)
Porcine
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(4)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 526. Show All 526 Publications.
Publications using 210-TA Applications Species
V Tisato, S Gallo, E Melloni, C Celeghini, A Passaro, G Zauli, P Secchiero, C Bergamini, A Trentini, G Bonaccorsi, G Valacchi, G Zuliani, C Cervellati TRAIL and Ceruloplasmin Inverse Correlation as a Representative Crosstalk between Inflammation and Oxidative Stress Mediators Inflamm., 2018;2018(0):9629537. 2018 [PMID: 30147446] (Bioassay, Human) Bioassay Human
É Boros, Z Kellermaye, P Balogh, G Strifler, A Vörös, P Sarlós, Á Vincze, C Varga, I Nagy Elevated Expression of AXL May Contribute to the Epithelial-to-Mesenchymal Transition in Inflammatory Bowel Disease Patients Mediators Inflamm., 2018;2018(0):3241406. 2018 [PMID: 30140167] (Bioassay, Human) Bioassay Human
M Schwede, EM Wilfong, RL Zemans, PJ Lee, C Dos Santos, X Fang, MA Matthay Effects of bone marrow-derived mesenchymal stromal cells on gene expression in human alveolar type II cells exposed to TNF-?, IL-1?, and IFN-? Physiol Rep, 2018;6(16):e13831. 2018 [PMID: 30136410] (Bioassay, Human) Bioassay Human
AL Means, TJ Freeman, J Zhu, LG Woodbury, P Marincola-, C Wu, AR Meyer, CJ Weaver, C Padmanabha, H An, J Zi, BC Wessinger, R Chaturvedi, TD Brown, NG Deane, RJ Coffey, KT Wilson, JJ Smith, CL Sawyers, JR Goldenring, SV Novitskiy, MK Washington, C Shi, RD Beauchamp Epithelial Smad4 Deletion Up-Regulates Inflammation and Promotes Inflammation-Associated Cancer Cell Mol Gastroenterol Hepatol, 2018;6(3):257-276. 2018 [PMID: 30109253] (Bioassay, Mouse) Bioassay Mouse
SL Figenschau, E Knutsen, I Urbarova, C Fenton, B Elston, M Perander, ES Mortensen, KA Fenton ICAM1 expression is induced by proinflammatory cytokines and associated with TLS formation in aggressive breast cancer subtypes Sci Rep, 2018;8(1):11720. 2018 [PMID: 30082828] (Bioassay, Human) Bioassay Human
S Ishii, T Isozaki, H Furuya, H Takeuchi, Y Tsubokura, K Inagaki, T Kasama ADAM-17 is expressed on rheumatoid arthritis fibroblast-like synoviocytes and regulates proinflammatory mediator expression and monocyte adhesion Arthritis Res. Ther., 2018;20(1):159. 2018 [PMID: 30071898] (Bioassay, Human) Bioassay Human
SI Jeong, JW Kim, KP Ko, BK Ryu, MG Lee, HJ Kim, SG Chi XAF1 forms a positive feedback loop with IRF-1 to drive apoptotic stress response and suppress tumorigenesis Cell Death Dis, 2018;9(8):806. 2018 [PMID: 30042418] (Bioassay, Human) Bioassay Human
JJ Seeley, RG Baker, G Mohamed, T Bruns, MS Hayden, SD Deshmukh, DE Freedberg, S Ghosh Induction of innate immune memory via microRNA targeting of chromatin remodelling factors Nature, 2018;559(7712):114-119. 2018 [PMID: 29950719] (Bioassay, Mouse) Bioassay Mouse
G Genard, AC Wera, C Huart, B Le Calve, S Penninckx, A Fattacciol, T Tabarrant, C Demazy, N Ninane, AC Heuskin, S Lucas, C Michiels Proton irradiation orchestrates macrophage reprogramming through NF?B signaling Cell Death Dis, 2018;9(7):728. 2018 [PMID: 29950610] (Bioassay, Human) Bioassay Human
W Feng, Y Wu, G Chen, S Fu, B Li, B Huang, D Wang, W Wang, J Liu Sodium Butyrate Attenuates Diarrhea in Weaned Piglets and Promotes Tight Junction Protein Expression in Colon in a GPR109A-Dependent Manner Cell. Physiol. Biochem., 2018;47(4):1617-1629. 2018 [PMID: 29949795] (Bioassay, Human) Bioassay Human
Show All 526 Publications.

Review for TNF-alpha (210-TA) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 210-TA:
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reviewed by:
Anonymous
05/09/2018
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Summary

Lot87325

FAQs for TNF-alpha (210-TA). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Anonymous
05/09/2018
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Bioinformatics

Gene Symbol TNF
Uniprot