Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

Western Blot: Human Pancreas Whole Tissue Lysate (Adult Whole Normal) [NB820-59244] - man Pancreas Whole Tissue Lysate (Adult Normal) [NB820-59244] - Image from verified customer review.

• Reactivity: Hu
• Applications: WB, IP
• Concentration: 5.0 mg/ml

Human Pancreas Whole Tissue Lysate (Adult Whole Normal) Summary

• Specificity: Total Protein - Human Adult Normal Tissue: Pancreas

Applications/Dilutions

• Dilutions:
  • Western Blot 10 ug - 20 ug
• Application Notes: Western Blot (10 ug to 20 ug per lane), Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent and a cocktail of protease inhibitors.
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Digestive/Waste
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Pancreas Lysate (NB820-59244)(1)

Publication using NB820-59244

• Doran TM, Morimoto J, Simanski S et al. Discovery of Phosphorylated Peripherin as a Major Humoral Autoantigen in Type 1 Diabetes Mellitus. Cell Chem Biol. May 19 2016 [PMID: 27185639] (IP)

Review for Pancreas Lysate (NB820-59244) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ming Lu WB Human 09/15/2014

Summary

• Application: Western Blot
• Sample Tested: Human normal pancreas lysate
• Species: Human
• Lot: B607124
• Comments: The lysate came pink, indicating there is blood mixed. This is the reason I give it a 4-star instead of 5-star. Otherwise it is good.

Blocking

• Blocking Details: 2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

• Dilution Ratio: b-actin (Novus NBP1-47423), 1:1000, 4C overnight in blocking buffer

Secondary Antibody

• Secondary Description: Goat anti-mouse IgG, HRP
• Secondary Manufacturer Cat#: NB7539
• Secondary Concentration: 1:5000

Details

• Detection Notes: very strong signal, clear background, exposure of 10 seconds is enough

Comments

• Comments: The lysate came pink, indicating there is blood mixed. This is the reason I give it a 4-star instead of 5-star. Otherwise it is good.

FAQs for Pancreas Lysate (NB820-59244). (Showing 1 - 2 of 2 FAQ).

1. Would you provide these information listed below? 1. The procedure of this lysate preparation. 2. The recipes of the buffer. ( component and concentration) In the data sheet, it only list the component: HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
   • The lysate was prepared using Biochain's total protein extraction kit (cat # K3011010). The buffer composition is listed on the datasheet however I am unable to disclose the amount of each component as that information is proprietary.
2. I am interested in Whole Normal Pancreas Tissue Lysate (Adult Normal) (NB820-59244). Would you please let me know how much protein you recommend to load on the gel?
   • This lysate should be prepared and ready to load in Western Blot. We recommend loading 20-40 ug of protein into your well to use as a positive control.