Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

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Western Blot: Human Pancreas Whole Tissue Lysate (Adult Whole Normal) [NB820-59244] - man Pancreas Whole Tissue Lysate (Adult Normal) [NB820-59244] - Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Pancreas Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Pancreas

Applications/Dilutions

Dilutions
  • Western Blot 10 ug - 20 ug
Application Notes
Western Blot (10 ug to 20 ug per lane), Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Reviewed Applications
Read 1 Review rated 4
using
NB820-59244 in the following application:

Publications
Read Publication using NB820-59244.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent and a cocktail of protease inhibitors.
Concentration
5.0 mg/ml

Lysate Details for Pancreas

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Pancreas Lysate (NB820-59244)(1)

We have publications tested in 1 application: IP.


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Review for Pancreas Lysate (NB820-59244) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB820-59244:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Pancreas NB820-59244
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reviewed by:
Ming Lu
WB Human 09/15/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman normal pancreas lysate
SpeciesHuman
LotB607124
CommentsThe lysate came pink, indicating there is blood mixed. This is the reason I give it a 4-star instead of 5-star. Otherwise it is good.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratiob-actin (Novus NBP1-47423), 1:1000, 4C overnight in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#NB7539
Secondary Concentration1:5000

Details

Detection Notesvery strong signal, clear background, exposure of 10 seconds is enough

Comments

CommentsThe lysate came pink, indicating there is blood mixed. This is the reason I give it a 4-star instead of 5-star. Otherwise it is good.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Pancreas Lysate (NB820-59244). (Showing 1 - 2 of 2 FAQ).

  1. Would you provide these information listed below? 1. The procedure of this lysate preparation. 2. The recipes of the buffer. ( component and concentration) In the data sheet, it only list the component: HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
    • The lysate was prepared using Biochain's total protein extraction kit (cat # K3011010). The buffer composition is listed on the datasheet however I am unable to disclose the amount of each component as that information is proprietary.
  2. I am interested in Whole Normal Pancreas Tissue Lysate (Adult Normal) (NB820-59244). Would you please let me know how much protein you recommend to load on the gel?
    • This lysate should be prepared and ready to load in Western Blot. We recommend loading 20-40 ug of protein into your well to use as a positive control.

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Recent Reviews

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Ming Lu
09/15/2014
Application: WB
Species: Human