Human Pancreas Whole Tissue Lysate (Adult Whole Normal) Summary
Total Protein - Human Adult Normal Tissue: Pancreas
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent and a cocktail of protease inhibitors.
Western Blot 10 ug - 20 ug Immunoprecipitation
Western Blot (10 ug to 20 ug per lane), Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Lysate Details for Pancreas
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
for 6 months from date of receipt.
Publications for Pancreas Lysate (NB820-59244)(1)
We have publications tested in 1 application: IP.
Submit a Publication
Filter By Application
Filter By Species
Showing Publication 1 -
Pancreas Lysate (NB820-59244) (1)
Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.
Submit a Review
Reviews using NB820-59244:
Filter by Applications
Filter by Species
Application Western Blot Sample Tested Human normal pancreas lysate Species Human Lot B607124 Comments The lysate came pink, indicating there is blood mixed. This is the reason I give it a 4-star instead of 5-star. Otherwise it is good. Blocking
Blocking Details 2% cold fish geletin in TBS buffer, room temperature for 1hour Primary Anitbody
Dilution Ratio b-actin (Novus NBP1-47423), 1:1000, 4C overnight in blocking buffer Secondary Antibody
Secondary Description Goat anti-mouse IgG, HRP Secondary Manufacturer Cat# NB7539 Secondary Concentration 1:5000 Details
Detection Notes very strong signal, clear background, exposure of 10 seconds is enough Comments
Comments The lysate came pink, indicating there is blood mixed. This is the reason I give it a 4-star instead of 5-star. Otherwise it is good.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Pancreas Lysate (NB820-59244). (Showing 1 -
2 of 2 FAQ).
Would you provide these information listed below? 1. The procedure of this lysate preparation. 2. The recipes of the buffer. ( component and concentration) In the data sheet, it only list the component: HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
The lysate was prepared using Biochain's total protein extraction kit (cat # K3011010). The buffer composition is listed on the datasheet however I am unable to disclose the amount of each component as that information is proprietary.
I am interested in Whole Normal Pancreas Tissue Lysate (Adult Normal) (NB820-59244). Would you please let me know how much protein you recommend to load on the gel?
This lysate should be prepared and ready to load in Western Blot. We recommend loading 20-40 ug of protein into your well to use as a positive control.
Additional Pancreas Products
Blogs on Pancreas