Mouse Erythropoietin/EPO Quantikine ELISA Kit

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse Erythropoietin/EPO Quantikine ELISA Kit Summary

Background
The Quantikine Mouse Epo Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Epo in cell culture supernates, tissue homogenates, serum, and plasma. It contains antibodies raised against recombinant Epo and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Epo showed linear curves that were parallel to the standard curves ob...tained using the Quantikine kit standards. These results indicate that the Quantikine Mouse Epo kit can be used to determine relative mass values for naturally occurring mouse Epo.
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Specificity
Natural and recombinant mouse Epo. Recombinant rat Epo has 100% cross-reactivity in this kit. This kit is not validated for use with rat samples, because rat sample dilution is not linear.
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Epo

Applications/Dilutions

Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using MEP00B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Erythropoietin/EPO Quantikine ELISA Kit

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Erythropoietin/EPO (MEP00B)(77)

We have publications tested in 2 confirmed species: Mouse, Rat.


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Mouse
(73)
Rat
(4)
All Species
Showing Publications 1 - 10 of 77. Show All 77 Publications.
Publications using MEP00B Applications Species
EM Buhl, S Djudjaj, BM Klinkhamme, K Ermert, VG Puelles, MT Lindenmeye, CD Cohen, C He, E Borkham-Ka, R Weiskirche, B Denecke, P Trairatphi, J Saez-Rodri, TB Huber, LE Olson, J Floege, P Boor Dysregulated mesenchymal PDGFR-&beta drives kidney fibrosis EMBO Mol Med, 2020;0(0):e11021. 2020 [PMID: 31943786] (Mouse) Mouse
Nagati J, Xu M, Garcia T, Comerford S, Hammer R, Garcia J A substitution mutation in a conserved domain of mammalian acetate-dependent acetyl CoA synthetase 2 results in destabilized protein and impaired HIF-2 signaling. PLoS ONE, 0;14(11):e0225105. 0 [PMID: 31725783] (Mouse) Mouse
C Casu, R Chessa, A Liu, R Gupta, H Drakesmith, R Fleming, YZ Ginzburg, B MacDonald, S Rivella Minihepcidins improve ineffective erythropoiesis and splenomegaly in a new mouse model of adult beta-thalassemia major Haematologica, 2019;0(0):. 2019 [PMID: 31582543] (Mouse) Mouse
S Altamura, NM Vegi, PS Hoppe, T Schroeder, M Aichler, A Walch, K Okreglicka, L Hültner, M Schneider, C Ladinig, C Kuklik-Roo, J Mysliwietz, D Janik, F Neff, B Rathkolb, M Hrabé de A, C Buske, AR da Silva, K Muedder, M Conrad, T Ganz, M Kopf, MU Muckenthal, GW Bornkamm Glutathione peroxidase 4 and vitamin E control reticulocyte maturation, stress erythropoiesis and iron homeostasis Haematologica, 2019;0(0):. 2019 [PMID: 31248967] (Mouse) Mouse
K Itoh, S Masumori, D Mukai, H Sakakibara, M Yasuda, K Shimoi Dosage time affects alkylating agents induced micronuclei in mouse peripheral blood reticulocytes through the function of erythropoietin J Toxicol Sci, 2019;44(4):273-282. 2019 [PMID: 30944280] (Mouse) Mouse
X Wang, M Zhang, SRL Flores, RR Woloshun, C Yang, L Yin, P Xiang, X Xu, MD Garrick, S Vidyasagar, D Merlin, JF Collins Oral Gavage of Ginger Nanoparticle-Derived Lipid Vectors Carrying Dmt1 siRNA Blunts Iron Loading in Murine Hereditary Hemochromatosis Mol. Ther., 2019;0(0):. 2019 [PMID: 30713087] (Mouse) Mouse
S Carvalho, M Ferrini, L Herritt, A Holian, Z Jaffar, K Roberts Multi-Walled Carbon Nanotubes Augment Allergic Airway Eosinophilic Inflammation by Promoting Cysteinyl Leukotriene Production Front Pharmacol, 2018;9(0):585. 2018 [PMID: 29922162] (Mouse) Mouse
TL Chen, YW Chiang, GL Lin, HH Chang, TS Lien, MH Sheh, DS Sun Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis Stem Cell Res Ther, 2018;9(1):119. 2018 [PMID: 29720275] (Mouse) Mouse
R Agoro, A Montagna, R Goetz, O Aligbe, G Singh, LM Coe, M Mohammadi, S Rivella, D Sitara Inhibition of fibroblast growth factor 23 (FGF23) signaling rescues renal anemia FASEB J., 2018;0(0):fj201700667R. 2018 [PMID: 29481308] (Mouse) Mouse
I Flamme, P Ellinghaus, D Urrego, T Krüger FGF23 expression in rodents is directly induced via erythropoietin after inhibition of hypoxia inducible factor proline hydroxylase PLoS ONE, 2017;12(10):e0186979. 2017 [PMID: 29073196] (Rat) Rat
Show All 77 Publications.

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Product General Protocols

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FAQs for Erythropoietin/EPO (MEP00B). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP MEP00B

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Bioinformatics

Gene Symbol Epo