Mouse Erythropoietin/EPO Quantikine ELISA Kit

Images

 

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Mouse Erythropoietin/EPO Quantikine ELISA Kit Summary

Background
The Quantikine Mouse Epo Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Epo in cell culture supernates, tissue homogenates, serum, and plasma. It contains antibodies raised against recombinant Epo and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Epo showed linear curves that were parallel to the standard curves ob...tained using the Quantikine kit standards. These results indicate that the Quantikine Mouse Epo kit can be used to determine relative mass values for naturally occurring mouse Epo.
Show More
Specificity
Natural and recombinant mouse Epo. Recombinant rat Epo has 100% cross-reactivity in this kit. This kit is not validated for use with rat samples, because rat sample dilution is not linear.
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Epo

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using MEP00B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Erythropoietin/EPO Quantikine ELISA Kit

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (Epo) is a 34-39 kDa secreted glycoprotein that is a member of the type I cytokine superfamily. The mouse Epo gene encodes a 192 amino acid (aa) residue precursor that contains a 26 aa signal peptide and a 166 aa mature protein containing three potential N-linked glycosylation sites (1-4). Mouse Epo lacks the O-linked glycosylation site found in human Epo. Although carbohydrate chains are not required for in vitro receptor binding, they are required for in vivo Epo bioactivity. Depending on the cell source, different Epo isoforms are produced that differ in their glycan compositions and sialic acid contents (5-8). Mature mouse and rat Epo share 94% aa sequence identity. They also share from 80%-82% aa identity with mature human, porcine, rhesus monkey and feline Epo (2, 3). Epo is primarily produced by cells in the kidney (interstitial peritubular renal fibroblasts) and liver (hepatocytes and Ito cells), where its production is up-regulated by hypoxia. Other tissues and cells, including neural tissues (astrocytes and neurons), testis (Sertoli cells), uterus, placenta, and erythroid progenitors, have also been shown to produce Epo (9-14). 
Epo is best known for its role in red blood cell formation. While Epo is not a lineage commitment factor, it inhibits apoptosis and induces burst forming unit-erythroid (BFU-E) differentiation into colony forming unit-erythroid (CFU-E), and the subsequent proliferation and maturation of CFU-E into early normoblasts (10, 15, 16). Apart from its role in erythropoiesis, Epo also acts on various non-hematopoietic cells to function as a viability and proliferation factor. Epo can stimulate myoblast proliferation while suppressing its differentiation, resulting in the expansion of the progenitor cell population (17). Epo is a tissueprotective factor that protects against ischemic and toxic injuries to neuronal, cardiovascular and renal tissues (18, 19). Epo has also been shown to promote angiogenesis in various physiologic and pathologic conditions (20, 21). 
Epo binds and signals via the high-affinity preformed homodimeric Epo receptor (Epo R) that is composed of two Epo R subunits. Each Epo R subunit is a type I transmembrane glycoprotein that belongs to the type I cytokine receptor superfamily (18, 22-24). Its extracellular domain contains the characteristic two fibronectin type III domains and a WSxWS motif near the plasma membrane (24, 25). Binding of Epo to the Epo R homodimer results in conformational change and phosphorylation and activation of the non-receptor protein kinase JAK2, which activates the downstream signaling cascade (26). An alternative Epo heteromeric receptor complex that transduces cell-protective signals and containing the beta common receptor ( beta CR) subunit in addition to the Epo R subunit has been described. beta CR also belongs to the type I cytokine receptor superfamily and is a subunit that is shared by the heteromeric IL-3, IL-5 and GM-CSF receptor complexes. Epo binds with lower affinity to the heteromeric receptor consisting of a Epo R subunit and a beta CR homodimer (18).

Customers Who Viewed This Item Also Viewed...

203-IL/CF
Species: Hu
Applications: BA
255-SC/CF
Species: Hu
Applications: BA
NBP1-19388
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
D6050
Species: Hu
Applications: ELISA
7954-GM/CF
Species: Hu
Applications: BA
210-TA
Species: Hu
Applications: BA
DVE00
Species: Hu
Applications: ELISA
MCS00
Species: Mu
Applications: ELISA
NBP2-59451
Species: Hu
Applications: ELISA, Flow, ICC/IF, MiAr, WB
2914-HT
Species: Hu
Applications: BA
7954-GM/CF
Species: Hu
Applications: BA
288-TPN/CF
Species: Hu
Applications: BA
MCS00
Species: Mu
Applications: ELISA
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
AF2168
Species: Hu, Mu
Applications: ChIP, Simple Western, WB
NB100-105
Species: Bv, Ca, Fe, Ma, Hu, Pm, Mu, Po, Pm, Rb, Rt, Sh, Xp
Applications: ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, LA, PLA, Simple Western, TCS, WB
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF1584
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IP, ICFlow, KO, Simple Western, WB
NLS3890
Species: Bv, Hu, Pm, Pm
Applications: IHC, IHC-P
MEP00B
Species: Mu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Erythropoietin/EPO (MEP00B)(98)

We have publications tested in 2 confirmed species: Mouse, Rat.


Filter By Application
All Applications
Filter By Species
Mouse
(93)
Rat
(4)
All Species
Showing Publications 1 - 10 of 98. Show All 98 Publications.
Publications using MEP00B Applications Species
E Hamza, M Vallejo-Mu, H Ouled-Hadd, C García-Cab, M Guerrero-H, L Santier, S Rayego-Mat, IA Larabi, JC Alvarez, L Garçon, ZA Massy, G Choukroun, JA Moreno, L Metzinger, VM Meuth Indoxyl sulfate impairs erythropoiesis at BFU-E stage in chronic kidney disease Cellular Signalling, 2022;0(0):110583. 2022 [PMID: 36596353] (Mouse) Mouse
VP Kumar, S Biswas, GP Holmes-Ham, M Sheleg, S Stone, B Legesse, R Ofir, SP Ghosh Pre-Administration of PLX-R18 Cells Protects Mice from Radiation-Induced Hematopoietic Failure and Lethality Genes, 2022;13(10):. 2022 [PMID: 36292639] (Mouse) Mouse
J Tapio, R Halmetoja, EY Dimova, JM Mäki, A Laitala, G Walkinshaw, J Myllyharju, R Serpi, P Koivunen Contribution of HIF-P4H isoenzyme inhibition to metabolism indicates major beneficial effects being conveyed by HIF-P4H-2 antagonism The Journal of Biological Chemistry, 2022;0(0):102222. 2022 [PMID: 35787374] (Mouse) Mouse
N Sissala, E Myllymäki, F Mohr, R Halmetoja, P Kuvaja, EY Dimova, P Koivunen Hypoxia ameliorates maternal diet-induced insulin resistance during pregnancy while having a detrimental effect on the placenta Physiological Reports, 2022;10(9):e15302. 2022 [PMID: 35535947] (Mouse) Mouse
MR Hanudel, B Czaya, S Wong, G Jung, K Chua, B Qiao, V Gabayan, T Ganz Renoprotective effects of ferric citrate in a mouse model of chronic kidney disease Scientific Reports, 2022;12(1):6695. 2022 [PMID: 35461329] (Mouse) Mouse
D Zhang, X Gao, H Li, DK Borger, Q Wei, E Yang, C Xu, S Pinho, PS Frenette The microbiota regulates hematopoietic stem cell fate decisions by controlling iron availability in bone marrow Cell Stem Cell, 2022;0(0):. 2022 [PMID: 35065706] (Mouse) Mouse
J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation Nature Communications, 2021;12(1):6963. 2021 [PMID: 34845225] (Mouse) Mouse
HM Shih, SY Pan, CJ Wu, YH Chou, CY Chen, FC Chang, YT Chen, WC Chiang, HC Tsai, YM Chen, SL Lin Transforming growth factor-beta1 decreases erythropoietin production through repressing hypoxia-inducible factor 2alpha in erythropoietin-producing cells Journal of Biomedical Science, 2021;28(1):73. 2021 [PMID: 34724959] (Mouse) Mouse
R Torosyan, S Huang, PV Bommi, R Tiwari, SY An, M Schonfeld, G Rajendran, MA Kavanaugh, B Gibbs, AD Truax, S Bohney, MW Calcutt, EW Kerr, R Leonardi, P Gao, NS Chandel, PP Kapitsinou Hypoxic preconditioning protects against ischemic kidney injury through the IDO1/kynurenine pathway Cell Reports, 2021;36(7):109547. 2021 [PMID: 34407414] (Mouse) Mouse
A Mattebo, T Sen, M Jassinskaj, K Pimková, I Prieto Gon, AG Alattar, R Ramakrishn, S Lang, M Järås, J Hansson, S Soneji, S Singbrant, E van den Ak, J Flygare Yippee like 4 (Ypel4) is essential for normal mouse red blood cell membrane integrity Scientific Reports, 2021;11(1):15898. 2021 [PMID: 34354145] (Mouse) Mouse
Show All 98 Publications.

Reviews for Erythropoietin/EPO (MEP00B) (0)

There are no reviews for Erythropoietin/EPO (MEP00B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Erythropoietin/EPO (MEP00B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Erythropoietin/EPO Products

Bioinformatics Tool for Erythropoietin/EPO (MEP00B)

Discover related pathways, diseases and genes to Erythropoietin/EPO (MEP00B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Erythropoietin/EPO (MEP00B)

Discover more about diseases related to Erythropoietin/EPO (MEP00B).
 

Pathways for Erythropoietin/EPO (MEP00B)

View related products by pathway.

PTMs for Erythropoietin/EPO (MEP00B)

Learn more about PTMs related to Erythropoietin/EPO (MEP00B).

Blogs on Erythropoietin/EPO

There are no specific blogs for Erythropoietin/EPO, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Mouse Erythropoietin/EPO Quantikine ELISA Kit and receive a gift card or discount.

Bioinformatics

Gene Symbol Epo