Mouse Erythropoietin/EPO Quantikine ELISA Kit



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Reactivity MuSpecies Glossary
Applications ELISA

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Mouse Erythropoietin/EPO Quantikine ELISA Kit Summary

The Quantikine Mouse Epo Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Epo in cell culture supernates, tissue homogenates, serum, and plasma. It contains antibodies raised against recombinant Epo and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Epo showed linear curves that were parallel to the standard curves ob...tained using the Quantikine kit standards. These results indicate that the Quantikine Mouse Epo kit can be used to determine relative mass values for naturally occurring mouse Epo.
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Natural and recombinant mouse Epo. Recombinant rat Epo has 100% cross-reactivity in this kit. This kit is not validated for use with rat samples, because rat sample dilution is not linear.
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Spike Recovery
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Sample Volume
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Application Notes
Interference observed with 1 or more available related molecules.
Read Publications using MEP00B.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Erythropoietin/EPO Quantikine ELISA Kit

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2


Erythropoietin (Epo) is a 34-39 kDa secreted glycoprotein that is a member of the type I cytokine superfamily. The mouse Epo gene encodes a 192 amino acid (aa) residue precursor that contains a 26 aa signal peptide and a 166 aa mature protein containing three potential N-linked glycosylation sites (1-4). Mouse Epo lacks the O-linked glycosylation site found in human Epo. Although carbohydrate chains are not required for in vitro receptor binding, they are required for in vivo Epo bioactivity. Depending on the cell source, different Epo isoforms are produced that differ in their glycan compositions and sialic acid contents (5-8). Mature mouse and rat Epo share 94% aa sequence identity. They also share from 80%-82% aa identity with mature human, porcine, rhesus monkey and feline Epo (2, 3). Epo is primarily produced by cells in the kidney (interstitial peritubular renal fibroblasts) and liver (hepatocytes and Ito cells), where its production is up-regulated by hypoxia. Other tissues and cells, including neural tissues (astrocytes and neurons), testis (Sertoli cells), uterus, placenta, and erythroid progenitors, have also been shown to produce Epo (9-14). 
Epo is best known for its role in red blood cell formation. While Epo is not a lineage commitment factor, it inhibits apoptosis and induces burst forming unit-erythroid (BFU-E) differentiation into colony forming unit-erythroid (CFU-E), and the subsequent proliferation and maturation of CFU-E into early normoblasts (10, 15, 16). Apart from its role in erythropoiesis, Epo also acts on various non-hematopoietic cells to function as a viability and proliferation factor. Epo can stimulate myoblast proliferation while suppressing its differentiation, resulting in the expansion of the progenitor cell population (17). Epo is a tissueprotective factor that protects against ischemic and toxic injuries to neuronal, cardiovascular and renal tissues (18, 19). Epo has also been shown to promote angiogenesis in various physiologic and pathologic conditions (20, 21). 
Epo binds and signals via the high-affinity preformed homodimeric Epo receptor (Epo R) that is composed of two Epo R subunits. Each Epo R subunit is a type I transmembrane glycoprotein that belongs to the type I cytokine receptor superfamily (18, 22-24). Its extracellular domain contains the characteristic two fibronectin type III domains and a WSxWS motif near the plasma membrane (24, 25). Binding of Epo to the Epo R homodimer results in conformational change and phosphorylation and activation of the non-receptor protein kinase JAK2, which activates the downstream signaling cascade (26). An alternative Epo heteromeric receptor complex that transduces cell-protective signals and containing the beta common receptor ( beta CR) subunit in addition to the Epo R subunit has been described. beta CR also belongs to the type I cytokine receptor superfamily and is a subunit that is shared by the heteromeric IL-3, IL-5 and GM-CSF receptor complexes. Epo binds with lower affinity to the heteromeric receptor consisting of a Epo R subunit and a beta CR homodimer (18).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for Erythropoietin/EPO (MEP00B)(102)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

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Showing Publications 1 - 10 of 102. Show All 102 Publications.
Publications using MEP00B Applications Species
Holmes-Hampton, GP;Kumar, VP;Biswas, S;Stone, S;Sharma, NK;Legesse, B;Vercellino, J;Guha, C;Eichenbaum, G;Ghosh, SP; PEGylated thrombopoietin mimetic, JNJ?26366821 a novel prophylactic radiation countermeasure for acute radiation injury Scientific reports 2023-09-14 [PMID: 37709916] (Mouse) Mouse
Matte, A;Wilson, AB;Gevi, F;Federti, E;Recchiuti, A;Ferri, G;Brunati, AM;Pagano, MA;Russo, R;Leboeuf, C;Janin, A;Timperio, AM;Iolascon, A;Gremese, E;Dang, L;Mohandas, N;Brugnara, C;De Franceschi, L; Mitapivat reprograms red cell metabolome and improves anemia in a mouse model of hereditary spherocytosis JCI insight 2023-09-07 [PMID: 37676741] (Transgenic Mouse, Mouse) Transgenic Mouse, Mouse
M Subramania, J McIninch, I Zlatev, MK Schlegel, C Kaittanis, T Nguyen, S Agarwal, T Racie, MA Alvarado, K Wassarman, TS Collins, T Chickering, CR Brown, K Schmidt, AB Castoreno, S Shulga-Mor, E Stamenova, K Buckowing, D Berman, JD Barry, A Bisbe, MA Maier, K Fitzgerald, V Jadhav RNAi-mediated rheostat for dynamic control of AAV-delivered transgenes Nature Communications, 2023-04-08;14(1):1970. 2023-04-08 [PMID: 37031257] (Mouse) Mouse
E Hamza, M Vallejo-Mu, H Ouled-Hadd, C García-Cab, M Guerrero-H, L Santier, S Rayego-Mat, IA Larabi, JC Alvarez, L Garçon, ZA Massy, G Choukroun, JA Moreno, L Metzinger, VM Meuth Indoxyl sulfate impairs erythropoiesis at BFU-E stage in chronic kidney disease Cellular Signalling, 2022-12-31;0(0):110583. 2022-12-31 [PMID: 36596353] (Mouse) Mouse
J Yang, Y Ruan, D Wang, J Fan, N Luo, H Chen, X Li, W Chen, X Wang VHL-recruiting PROTAC attenuates renal fibrosis and preserves renal function via simultaneous degradation of Smad3 and stabilization of HIF-2alpha Cell & bioscience, 2022-12-19;12(1):203. 2022-12-19 [PMID: 36536448] (Mouse) Mouse
VP Kumar, S Biswas, GP Holmes-Ham, M Sheleg, S Stone, B Legesse, R Ofir, SP Ghosh Pre-Administration of PLX-R18 Cells Protects Mice from Radiation-Induced Hematopoietic Failure and Lethality Genes, 2022-09-28;13(10):. 2022-09-28 [PMID: 36292639] (Mouse) Mouse
J Tapio, R Halmetoja, EY Dimova, JM Mäki, A Laitala, G Walkinshaw, J Myllyharju, R Serpi, P Koivunen Contribution of HIF-P4H isoenzyme inhibition to metabolism indicates major beneficial effects being conveyed by HIF-P4H-2 antagonism The Journal of Biological Chemistry, 2022-07-01;0(0):102222. 2022-07-01 [PMID: 35787374] (Mouse) Mouse
N Sissala, E Myllymäki, F Mohr, R Halmetoja, P Kuvaja, EY Dimova, P Koivunen Hypoxia ameliorates maternal diet-induced insulin resistance during pregnancy while having a detrimental effect on the placenta Physiological Reports, 2022-05-01;10(9):e15302. 2022-05-01 [PMID: 35535947] (Mouse) Mouse
MR Hanudel, B Czaya, S Wong, G Jung, K Chua, B Qiao, V Gabayan, T Ganz Renoprotective effects of ferric citrate in a mouse model of chronic kidney disease Scientific Reports, 2022-04-23;12(1):6695. 2022-04-23 [PMID: 35461329] (Mouse) Mouse
D Zhang, X Gao, H Li, DK Borger, Q Wei, E Yang, C Xu, S Pinho, PS Frenette The microbiota regulates hematopoietic stem cell fate decisions by controlling iron availability in bone marrow Cell Stem Cell, 2022-01-21;0(0):. 2022-01-21 [PMID: 35065706] (Mouse) Mouse
Show All 102 Publications.

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Gene Symbol Epo