The Quantikine Mouse Epo Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Epo in cell culture supernates, tissue homogenates, serum, and plasma. It contains antibodies raised against recombinant Epo and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Epo showed linear curves that were parallel to the standard curves ob...tained using the Quantikine kit standards. These results indicate that the Quantikine Mouse Epo kit can be used to determine relative mass values for naturally occurring mouse Epo.
Natural and recombinant mouse Epo. Recombinant rat Epo has 100% cross-reactivity in this kit. This kit is not validated for use with rat samples, because rat sample dilution is not linear.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Mouse Erythropoietin/EPO Quantikine ELISA Kit
Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.
The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
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