Mouse Erythropoietin/EPO Quantikine ELISA Kit

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Mouse Erythropoietin/EPO Quantikine ELISA Kit Summary

Background
The Quantikine Mouse Epo Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Epo in cell culture supernates, tissue homogenates, serum, and plasma. It contains antibodies raised against recombinant Epo and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Epo showed linear curves that were parallel to the standard curves ob...tained using the Quantikine kit standards. These results indicate that the Quantikine Mouse Epo kit can be used to determine relative mass values for naturally occurring mouse Epo.
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Specificity
Natural and recombinant mouse Epo. Recombinant rat Epo has 100% cross-reactivity in this kit. This kit is not validated for use with rat samples, because rat sample dilution is not linear.
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Epo

Applications/Dilutions

Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using MEP00B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Erythropoietin/EPO Quantikine ELISA Kit

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (Epo) is a 34-39 kDa secreted glycoprotein that is a member of the type I cytokine superfamily. The mouse Epo gene encodes a 192 amino acid (aa) residue precursor that contains a 26 aa signal peptide and a 166 aa mature protein containing three potential N-linked glycosylation sites (1-4). Mouse Epo lacks the O-linked glycosylation site found in human Epo. Although carbohydrate chains are not required for in vitro receptor binding, they are required for in vivo Epo bioactivity. Depending on the cell source, different Epo isoforms are produced that differ in their glycan compositions and sialic acid contents (5-8). Mature mouse and rat Epo share 94% aa sequence identity. They also share from 80%-82% aa identity with mature human, porcine, rhesus monkey and feline Epo (2, 3). Epo is primarily produced by cells in the kidney (interstitial peritubular renal fibroblasts) and liver (hepatocytes and Ito cells), where its production is up-regulated by hypoxia. Other tissues and cells, including neural tissues (astrocytes and neurons), testis (Sertoli cells), uterus, placenta, and erythroid progenitors, have also been shown to produce Epo (9-14). 
Epo is best known for its role in red blood cell formation. While Epo is not a lineage commitment factor, it inhibits apoptosis and induces burst forming unit-erythroid (BFU-E) differentiation into colony forming unit-erythroid (CFU-E), and the subsequent proliferation and maturation of CFU-E into early normoblasts (10, 15, 16). Apart from its role in erythropoiesis, Epo also acts on various non-hematopoietic cells to function as a viability and proliferation factor. Epo can stimulate myoblast proliferation while suppressing its differentiation, resulting in the expansion of the progenitor cell population (17). Epo is a tissueprotective factor that protects against ischemic and toxic injuries to neuronal, cardiovascular and renal tissues (18, 19). Epo has also been shown to promote angiogenesis in various physiologic and pathologic conditions (20, 21). 
Epo binds and signals via the high-affinity preformed homodimeric Epo receptor (Epo R) that is composed of two Epo R subunits. Each Epo R subunit is a type I transmembrane glycoprotein that belongs to the type I cytokine receptor superfamily (18, 22-24). Its extracellular domain contains the characteristic two fibronectin type III domains and a WSxWS motif near the plasma membrane (24, 25). Binding of Epo to the Epo R homodimer results in conformational change and phosphorylation and activation of the non-receptor protein kinase JAK2, which activates the downstream signaling cascade (26). An alternative Epo heteromeric receptor complex that transduces cell-protective signals and containing the beta common receptor ( beta CR) subunit in addition to the Epo R subunit has been described. beta CR also belongs to the type I cytokine receptor superfamily and is a subunit that is shared by the heteromeric IL-3, IL-5 and GM-CSF receptor complexes. Epo binds with lower affinity to the heteromeric receptor consisting of a Epo R subunit and a beta CR homodimer (18).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Erythropoietin/EPO (MEP00B)(80)

We have publications tested in 2 confirmed species: Mouse, Rat.


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Rat
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Showing Publications 1 - 10 of 80. Show All 80 Publications.
Publications using MEP00B Applications Species
J Varghese, JV James, R Anand, M Narayanasa, G Rebekah, B Ramakrishn, AJ Nellickal, M Jacob Development of insulin resistance preceded major changes in iron homeostasis in mice fed a high-fat diet J. Nutr. Biochem., 2020;84(0):108441. 2020 [PMID: 32629238] (Mouse) Mouse
N Chakrabort, A Gautam, GP Holmes-Ham, VP Kumar, S Biswas, R Kumar, D Hamad, G Dimitrov, AO Olabisi, R Hammamieh, SP Ghosh microRNA and Metabolite Signatures Linked to Early Consequences of Lethal Radiation Sci Rep, 2020;10(1):5424. 2020 [PMID: 32214144] (Mouse) Mouse
R Agoro, MY Park, C Le Henaff, S Jankauskas, A Gaias, G Chen, M Mohammadi, D Sitara C-FGF23 peptide alleviates hypoferremia during acute inflammation Haematologica, 2020;0(0):. 2020 [PMID: 32193252] (Mouse) Mouse
EM Buhl, S Djudjaj, BM Klinkhamme, K Ermert, VG Puelles, MT Lindenmeye, CD Cohen, C He, E Borkham-Ka, R Weiskirche, B Denecke, P Trairatphi, J Saez-Rodri, TB Huber, LE Olson, J Floege, P Boor Dysregulated mesenchymal PDGFR-&beta drives kidney fibrosis EMBO Mol Med, 2020;0(0):e11021. 2020 [PMID: 31943786] (Mouse) Mouse
Nagati J, Xu M, Garcia T, Comerford S, Hammer R, Garcia J A substitution mutation in a conserved domain of mammalian acetate-dependent acetyl CoA synthetase 2 results in destabilized protein and impaired HIF-2 signaling. PLoS ONE, 0;14(11):e0225105. 0 [PMID: 31725783] (Mouse) Mouse
C Casu, R Chessa, A Liu, R Gupta, H Drakesmith, R Fleming, YZ Ginzburg, B MacDonald, S Rivella Minihepcidins improve ineffective erythropoiesis and splenomegaly in a new mouse model of adult beta-thalassemia major Haematologica, 2019;0(0):. 2019 [PMID: 31582543] (Mouse) Mouse
S Altamura, NM Vegi, PS Hoppe, T Schroeder, M Aichler, A Walch, K Okreglicka, L Hültner, M Schneider, C Ladinig, C Kuklik-Roo, J Mysliwietz, D Janik, F Neff, B Rathkolb, M Hrabé de A, C Buske, AR da Silva, K Muedder, M Conrad, T Ganz, M Kopf, MU Muckenthal, GW Bornkamm Glutathione peroxidase 4 and vitamin E control reticulocyte maturation, stress erythropoiesis and iron homeostasis Haematologica, 2019;0(0):. 2019 [PMID: 31248967] (Mouse) Mouse
K Itoh, S Masumori, D Mukai, H Sakakibara, M Yasuda, K Shimoi Dosage time affects alkylating agents induced micronuclei in mouse peripheral blood reticulocytes through the function of erythropoietin J Toxicol Sci, 2019;44(4):273-282. 2019 [PMID: 30944280] (Mouse) Mouse
X Wang, M Zhang, SRL Flores, RR Woloshun, C Yang, L Yin, P Xiang, X Xu, MD Garrick, S Vidyasagar, D Merlin, JF Collins Oral Gavage of Ginger Nanoparticle-Derived Lipid Vectors Carrying Dmt1 siRNA Blunts Iron Loading in Murine Hereditary Hemochromatosis Mol. Ther., 2019;0(0):. 2019 [PMID: 30713087] (Mouse) Mouse
S Carvalho, M Ferrini, L Herritt, A Holian, Z Jaffar, K Roberts Multi-Walled Carbon Nanotubes Augment Allergic Airway Eosinophilic Inflammation by Promoting Cysteinyl Leukotriene Production Front Pharmacol, 2018;9(0):585. 2018 [PMID: 29922162] (Mouse) Mouse
Show All 80 Publications.

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Product General Protocols

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FAQs for Erythropoietin/EPO (MEP00B). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP MEP00B

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Bioinformatics

Gene Symbol Epo