HIF-2 alpha/EPAS1 Antibody (ep190b) Summary
Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]
This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1-2 ug/ml
- Simple Western 1:100
- Chromatin Immunoprecipitation
- ELISA 1:100-1:2000
- Flow Cytometry 1:400
- Immunohistochemistry 1:150-1:300
- Immunohistochemistry-Paraffin 1:150-1:300
- Gel Supershift Assay
This HIF-2 alpha (ep190b) antibody is useful for ELISA, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, it recognizes a band at approx. 118 kDa representing HIF-2 alpha. Use in Gel supper shift assay reported in scientific literature (PMID: 17404621)
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|Read Publications using
NB100-132 in the following applications:
Ability to use in mouse is mixed with some positive and some negative results.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS with 1% BSA
0.05% Sodium Azide
Protein G purified
Alternate Names for HIF-2 alpha/EPAS1 Antibody (ep190b)
- Basic-helix-loop-helix-PAS protein MOP2
- Class E basic helix-loop-helix protein 73
- endothelial PAS domain protein 1
- endothelial PAS domain-containing protein 1
- HIF 2A
- HIF-1alpha-like factor
- HIF2 alpha
- HIF-2 alpha
- hif2a angiogenesis
- HLFHIF-1-alpha-like factor
- hypoxia-inducible factor 2 alpha
- Hypoxia-inducible factor 2-alpha
- Member of PAS protein 2
- PASD2PAS domain-containing protein 2
Hypoxia-inducible factors (HIFs) generates heterodimers composed of alpha-subunit (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunit (Arnt1, Arnt2 and Arnt3). Of these isoforms, HIF-1 alpha and HIF-2 alpha play a critical role in the cellular response to hypoxic conditions. After heterodimerization with beta subunits and binding to DNA sequence 5'-[AG]CGTG-3' within HRE (hypoxia response element) of target gene promoters, HIF-2 alpha regulates the gene expression in response to oxygen homeostasis alterations. HIF-2 alpha regulates VEGF expression for involving in the development of new blood vessels as well as the tubular system of lung. HIF-2 alpha play a role in the formation of the endothelium that gives rise to blood brain barrier. HIF-2 alpha is a strong activator of expression of Tie-2 tyrosine kinase which is a major player in angiogenesis. HIF-2alpha activation require recruitment of transcriptional coactivators such as CREBPB, EP300 etc. and its interaction with redox regulatory protein APEX mediates activation of CTAD. HIF-2 alpha/HIF-1alpha expression increases in certain malignancies, wherein, HIF-1 alpha plays both pro- as well as anti-proliferative activities, whereas, HIF-2 alpha is suggested to exert anti-proliferative activity only.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
View specific protocols for HIF-2 alpha/EPAS1 Antibody (NB100-132):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for HIF-2 alpha/EPAS1 Antibody (NB100-132). (Showing 1 - 2 of 2 FAQs).
Do you have a specific protocol for using NB100-132 for western blot and a recipe for preparing cell lysates to test this antibody?
- Please find the specific protocol for NB100-132 here. This HIF antibody will require the standard hypoxic cell treatment.
I tried the antibody. By western blot on mouse T cells, I obtained a major non-specific band at 70KDa and a very weak band at 100KDa. Do you know what the 70KDa protein could be? However, I did this experiment on total cell lysates and I would like to redo this on nuclear extracts.
- We have also encountered a band at 70kDa in our testing on whole cell lysates. In our experience, this band usually does not respond to hypoxic induction, is only present in whole cell and cytoplasmic fractions, and is seen in almost all HIF1 alpha products that we have tested. We currently do not know the identity of this band, but we think it could be a HIF1 alpha degradation product.
Other Available Formats
Additional Array Products
Bioinformatics Tool for HIF-2 alpha/EPAS1 Antibody (NB100-132)
Discover related pathways, diseases and genes to HIF-2 alpha/EPAS1 Antibody (NB100-132). Need help?
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Blogs on HIF-2 alpha/EPAS1.