HIF-2 alpha/EPAS1 Antibody (ep190b)

Images

 
Biological Strategies: Simple Western: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under ...read more
Biological Strategies: Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - HRP conjugated HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 ...read more
Immunofluorescence: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Analysis of HIF-2 alpha in human endometrium using anti-HIF-2 alpha antibody. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image ...read more
Flow Cytometry: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - HIF-2 alpha antibody was tested at 1:400 in HepG2 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - [HRP] [NB100-132H] - Analysis of HIF-2 alpha stabilization over time in HOS cells following exposure to hypoxia. [PMID: 23785417] Image using the HRP form ...read more
Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Analysis of HIF-2 in human cardiac myocytes.
Biological Strategies: Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Analysis of HepG2 without Cobalt (II) Chloride (1), HepG2 with Cobalt (II) Chloride (2), HepG2 normoxic (3), HepG2 hypoxic ...read more
Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - C57B6 5 week old Mouse Kidney Tissue 60ug. Image from verified customer review.
Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Analysis of HIF-2 alpha stabilization over time in 791T cells following exposure to hypoxia. [PMID: 23785417] Image using the HRP form of this antibody.
Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Analysis of HIF-2 alpha in paraffin-embedded mouse kidney tissue using anti-HIF-2 alpha antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, GS, ICC/IF
Clone
ep190b
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

HIF-2 alpha/EPAS1 Antibody (ep190b) Summary

Immunogen
Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]
Specificity
This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPAS1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Simple Western 1:100
  • Chromatin Immunoprecipitation
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:400
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:150-1:300
  • Immunohistochemistry-Paraffin 1:150-1:300
  • Immunoprecipitation
  • Gel Supershift Assay
  • Immunofluorescence
Application Notes
This HIF-2 alpha (ep190b) antibody is useful for ELISA, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, it recognizes a band at approx. 118 kDa representing HIF-2 alpha. Use in Gel supper shift assay reported in scientific literature (PMID: 17404621)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Kidney Lysate (NB820-59231)
HIF-2 alpha/EPAS1 Lysate (NBL1-10286)
HIF-2 alpha/EPAS1 Lysate (NBP2-64985)
Reviewed Applications
Read 7 Reviews rated 4.3
using
NB100-132 in the following applications:

Publications
Read Publications using
NB100-132 in the following applications:

Reactivity Notes

Ability to use in mouse is mixed with some positive and some negative results.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS with 1% BSA
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HIF-2 alpha/EPAS1 Antibody (ep190b)

  • Basic-helix-loop-helix-PAS protein MOP2
  • BHLHE73
  • bHLHe73ECYT4
  • Class E basic helix-loop-helix protein 73
  • endothelial PAS domain protein 1
  • endothelial PAS domain-containing protein 1
  • ep190b
  • EPAS1
  • EPAS-1
  • HIF 2A
  • HIF-1alpha-like factor
  • HIF2 alpha
  • HIF-2 alpha
  • hif2a angiogenesis
  • HIF2A
  • HIF2AHIF-2-alpha
  • HLF
  • HLFHIF-1-alpha-like factor
  • hypoxia-inducible factor 2 alpha
  • Hypoxia-inducible factor 2-alpha
  • Member of PAS protein 2
  • MOP2
  • MOP2HIF2-alpha
  • PASD2PAS domain-containing protein 2

Background

Hypoxia-inducible factors (HIFs) generates heterodimers composed of alpha-subunit (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunit (Arnt1, Arnt2 and Arnt3). Of these isoforms, HIF-1 alpha and HIF-2 alpha play a critical role in the cellular response to hypoxic conditions. After heterodimerization with beta subunits and binding to DNA sequence 5'-[AG]CGTG-3' within HRE (hypoxia response element) of target gene promoters, HIF-2 alpha regulates the gene expression in response to oxygen homeostasis alterations. HIF-2 alpha regulates VEGF expression for involving in the development of new blood vessels as well as the tubular system of lung. HIF-2 alpha play a role in the formation of the endothelium that gives rise to blood brain barrier. HIF-2 alpha is a strong activator of expression of Tie-2 tyrosine kinase which is a major player in angiogenesis. HIF-2alpha activation require recruitment of transcriptional coactivators such as CREBPB, EP300 etc. and its interaction with redox regulatory protein APEX mediates activation of CTAD. HIF-2 alpha/HIF-1alpha expression increases in certain malignancies, wherein, HIF-1 alpha plays both pro- as well as anti-proliferative activities, whereas, HIF-2 alpha is suggested to exert anti-proliferative activity only.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-132
Species: Hu, Mu, Rt, Ha
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, GS, ICC/IF

Publications for HIF-2 alpha/EPAS1 Antibody (NB100-132)(156)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 9 applications: ChIP, ELISA, EMSA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ChIP
(9)
ELISA
(1)
EMSA
(1)
ICC/IF
(7)
IHC
(13)
IHC-Fr
(1)
IHC-P
(17)
IP
(4)
WB
(76)
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Filter By Species
Human
(76)
Mouse
(20)
Rat
(5)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 156. Show All 156 Publications.
Publications using NB100-132 Applications Species
Moreno Roig E, Yaromina A, Houben R et al. Prognostic Role of Hypoxia-Inducible Factor-2alpha Tumor Cell Expression in Cancer Patients: A Meta-Analysis Front Oncol Jun 11 2018 [PMID: 29942795] (IHC-P, Human) IHC-P Human
Lin Z, Liu F, Shi P et al. Fatty acid oxidation promotes reprogramming by enhancing oxidative phosphorylation and inhibiting protein kinase C. Stem Cell Res Ther. Feb 26 2018 [PMID: 29482657] (WB, Mouse) WB Mouse
Liu Y, Tan X, Liu W, Chen X. Follistatin-like protein 1 plays a tumor suppressor role in clear-cell renal cell carcinoma. Chin J Cancer. 2018 Jan 22 [PMID: 29357946] (IHC-P, Human) IHC-P Human
Chen R, Xu M, Nagati J, Garcia JA. Coordinate regulation of stress signaling and epigenetic events by Acss2 and HIF-2 in cancer cells. PLoS ONE 2017 Dec 27 [PMID: 29281714] (WB, IP, ChIP, Human) WB, IP, ChIP Human
Sadiku P, Willson JA, Dickinson RS et al. Prolyl hydroxylase 2 inactivation enhances glycogen storage and promotes excessive neutrophilic responses J. Clin. Invest. 2017 Aug 14 [PMID: 28805660] (IHC-P, Mouse) IHC-P Mouse
JA Walker. EXPRESSION OF HUMAN PAPILLOMAVIRUS TYPE 16 E7 IS SUFFICIENT TO SIGNIFICANTLY INCREASE EXPRESSSION OF ANGIOGENIC FACTORS BUT IS NOT SUFFICIENT TO INDUCE ENDOTHELIAL CELL MIGRATION. Indiana University, in partial fulfillment of the requirements for the degree of Doctor of Philosophy. Aug-10.
Carew JS, Espitia CM, Zhao W et al. Oncolytic reovirus inhibits angiogenesis through induction of CXCL10/IP-10 and abrogation of HIF activity in soft tissue sarcomas Oncotarget. 2017 Oct 17 [PMID: 29156834] (WB) WB
Padro M, Louie RJ, Lananna BV et al. Genome-independent hypoxic repression of estrogen receptor alpha in breast cancer cells. BMC Cancer. Mar 20 2017 [PMID: 28320353] (WB, ICC/IF, Human) WB, ICC/IF Human
Liu W, Chen H, Wong N et al. Pseudohypoxia induced by miR-126 deactivation promotes migration and therapeutic resistance in renal cell carcinoma. Cancer Lett. May 28 2017 [PMID: 28257806] (WB, Human) WB Human
Shiraishi A, Tachi K, Essid N et al. Hypoxia promotes the phenotypic change of aldehyde dehydrogenase activity of breast cancer stem cells. Cancer Sci. Mar 1 2017 [PMID: 28012234] (WB, Human) WB Human
Show All 156 Publications.

Reviews for HIF-2 alpha/EPAS1 Antibody (NB100-132) (7) 4.37

Average Rating: 4.3
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-132:
Filter by Applications
IF
(1)
WB
(5)
All Applications
Filter by Species
Human
(4)
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence HIF-2 alpha/EPAS1 NB100-132
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reviewed by:
Anonymous
IF Human 02/05/2016
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Summary

ApplicationImmunofluorescence
Sample TestedHuman endometrium
SpeciesHuman
Lotlot J-3

Blocking

Blocking Details10% normal serum

Primary Anitbody

Dilution Ratio1:250 in PBS, overnight incubation at 4 c

Secondary Antibody

Secondary Descriptionalexa fluor 488 donkey anti mouse
Secondary Manufacturer Cat#Jackson Immunolab
Secondary Concentration1:500

Details

Detection Notesnegative control without primary antibody
Fixation Detailsantigen retrieval
Wash Descriptionwash 3X PBS
Western Blot HIF-2 alpha/EPAS1 NB100-132
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reviewed by:
Darren Richard
WB Mouse 07/24/2015
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Summary

ApplicationWestern Blot
Sample TestedEndothelial
SpeciesMouse

Blocking

Blocking DetailsTBS-T, Milk

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Promega
Secondary Concentration1:2000

Details

Detection NotesHomemade ECL

Comments

CommentsA good choice for Western blots.
 HIF-2 alpha/EPAS1 NB100-132
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reviewed by:
Anonymous
Mouse 02/04/2015
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Summary

SpeciesMouse
FileView PDF
 
reviewed by:
Anonymous
WB Human 12/12/2014
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23549086
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23549086

Details

Detection NotesSee PMID 23549086

Comments

CommentsPublished in PMID:23549086.
Western Blot HIF-2 alpha/EPAS1 NB100-132
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reviewed by:
Salvador Macip
WB Human 08/11/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
Western Blot HIF-2 alpha/EPAS1 NB100-132
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reviewed by:
Anonymous
WB Mouse 07/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedMouse Kidney Tissue C57B5
SpeciesMouse
CommentsI used frozen tissue

Blocking

Blocking Details1 hr blocked in 10% bovine serum albumin in phosphate buffered saline (PBS) containing 0.1% (v/v) Tween-20 (PBS-T)

Primary Anitbody

Dilution Ratio1mg/ml 4C over night

Secondary Antibody

Secondary Descriptionanti rabbit
Secondary Concentration1:15,000

Details

Detection Noteswestern blots were developed using supersignal west femto maximum sensitivity

Comments

CommentsI used frozen tissue
 
reviewed by:
Anonymous
WB Human 12/14/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 1% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:2000

Details

Detection NotesDetection Method: ECL

Product General Protocols

View specific protocols for HIF-2 alpha/EPAS1 Antibody (NB100-132): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for HIF-2 alpha/EPAS1 Antibody (NB100-132). (Showing 1 - 2 of 2 FAQs).

  1. Do you have a specific protocol for using NB100-132 for western blot and a recipe for preparing cell lysates to test this antibody?
    • Please find the specific protocol for NB100-132 here. This HIF antibody will require the standard hypoxic cell treatment.
  2. I tried the antibody. By western blot on mouse T cells, I obtained a major non-specific band at 70KDa and a very weak band at 100KDa. Do you know what the 70KDa protein could be? However, I did this experiment on total cell lysates and I would like to redo this on nuclear extracts.
    • We have also encountered a band at 70kDa in our testing on whole cell lysates. In our experience, this band usually does not respond to hypoxic induction, is only present in whole cell and cytoplasmic fractions, and is seen in almost all HIF1 alpha products that we have tested. We currently do not know the identity of this band, but we think it could be a HIF1 alpha degradation product.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-132AF405
Alexa Fluor 488 Labeled NB100-132AF488
Alexa Fluor 647 Labeled NB100-132AF647
Alexa Fluor 700 Labeled NB100-132AF700
Allophycocyanin Labeled NB100-132APC
Biotin Labeled NB100-132B
DyLight 350 Labeled NB100-132UV
DyLight 405 Labeled NB100-132V
DyLight 488 Labeled NB100-132G
DyLight 550 Labeled NB100-132R
DyLight 650 Labeled NB100-132C
DyLight 680 Labeled NB100-132FR
DyLight 755 Labeled NB100-132IR
FITC Labeled NB100-132F
HRP Labeled NB100-132H
PE Labeled NB100-132PE
PerCP Labeled NB100-132PCP

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Blogs on HIF-2 alpha/EPAS1.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-2 alpha, Tumor Suppression and Cell Survival
HIF-2 alpha is one subunit within the HIF-2 nuclear protein that regulates cellular responses to hypoxia (low oxygen tension conditions). Hydroxylation post-translational modifications on particular HIF residues target them for degradation. Luo, et al...  Read full blog post.

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Recent Reviews

4.3
5
3
4
3
3
1
2
0
1
0

Anonymous
02/05/2016
Application: IF
Species: Human

Darren Richard
07/24/2015
Application: WB
Species: Mouse

Anonymous
02/04/2015
Application:
Species: Mouse

Bioinformatics

Gene Symbol EPAS1
Entrez
Uniprot