Mouse IgG1 Isotype Control (MG1)

SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.

• Reactivity: Mu
• Applications: WB, ELISA, FLISA, Flow, ICC/IF
• Clone: MG1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Mouse IgG1 Isotype Control (MG1) Summary

• Description: This Mouse IgG1 immunoglobulin is useful as an isotype control for Mouse IgG1 antibody.
• Specificity: In an Ouchterlony double diffusion assay, the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Mouse IgG1 Isotype Control (MG1)

• IgG1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG1 Isotype Control (NBP1-97005)(3)

Publications using NBP1-97005

• Herreno AM, Ramirez AC, Chaparro VP et al. Characterization of a functionally active primary microglial cell culture from the pig retina Exp. Eye Res. May 16 2019 [PMID: 31103710] (ICC/IF, Porcine)
• Siegel D, Dehn DD, Bokatzian SS et al. Redox modulation of NQO1 PLoS ONE 2018 Jan 03 [PMID: 29298345] (Mouse)
• Kusne Y, Carrera-Silva EA, Perry AS et al. Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFalpha in glioblastoma. Sci Signal 2014 Aug 12 [PMID: 25118327] (WB)

Review for IgG1 Isotype Control (NBP1-97005) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous Flow Human 04/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: human fetal derived neural stem cells
• Species: Human
• Lot: 28929

Blocking

• Blocking Details: N/A

Primary Anitbody

• Dilution Ratio: mixed in a 1:50 dilution of 1XPBS for 30 minutes at room temperature

Secondary Antibody

• Secondary Description: goat anti-mouse APC/Cy7
• Secondary Manufacturer Cat#: Abcam, ab130785
• Secondary Concentration: 1:50

Details

• Detection Notes: detected on flow cytometry, RL2 (750nm)
• Fixation Details: sample fixed in PFA for 15 minutes at room temperature
• Permeabilization Details: N/A
• Wash Description: samples were washed with 1XPBS at room temperatue, 3mL per wash

FAQs for IgG1 Isotype Control (NBP1-97005). (Showing 1 - 2 of 2 FAQs).

1. I would like to know the host strain of this product?
   • This isotype control was purified from ascites and consists of antibody from Balb/c with possible trace amounts from Swiss webster.
2. Could you please let me know the supply form (Liquid or Lyohpilized) of this antibody? 
   • Yes, it is liquid. Supplied in 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: none. Preservative: 0.01% Sodium Azide.