Mouse IgG1 Isotype Control (MG1)

Images

 
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF, PAGE
Clone
MG1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Mouse IgG1 Isotype Control (MG1) Summary

Specificity
Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100 - 1:2000
Application Notes
This product has been tested by ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-97005 in the following applications:

Publications
Read Publications using
NBP1-97005 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Notes

This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Mouse IgG1 Isotype Control (MG1)

  • IgG1
  • Igh-4
  • RGD1359539
  • VH7183

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG1 Isotype Control (NBP1-97005)(4)

Review for IgG1 Isotype Control (NBP1-97005) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-97005:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry IgG1 NBP1-97005
Enlarge
5
reviewed by:
Jessica Ochoa
Flow Human 04/21/2015
View

Summary

ApplicationFlow Cytometry
Sample Testedhuman fetal derived neural stem cells
SpeciesHuman
Lot28929

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG1 Isotype Control (NBP1-97005). (Showing 1 - 2 of 2 FAQs).

  1. I would like to know the host strain of this product?
    • This isotype control was purified from ascites and consists of antibody from Balb/c with possible trace amounts from Swiss webster.
  2. Could you please let me know the supply form (Liquid or Lyohpilized) of this antibody? 
    • Yes, it is liquid. Supplied in 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: none. Preservative: 0.01% Sodium Azide. 

Additional IgG1 Products

Research Areas for IgG1 Isotype Control (NBP1-97005)

Find related products by research area.

Blogs on IgG1.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Jessica Ochoa
04/21/2015
Application: Flow
Species: Human