This product has been tested by ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Read 1 Review rated 5 using NBP1-97005 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Protein A purified
This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Alternate Names for Mouse IgG1 Isotype Control (MG1)
IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
Publications for IgG1 Isotype Control (NBP1-97005)(4)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 3 applications: ICC/IF, IHC-FrFl, WB.
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