Mouse IgG1 Isotype Control (MG1)

SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.

• Reactivity: Mu
• Applications: WB, ELISA, FLISA, Flow, ICC/IF
• Clone: MG1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Mouse IgG1 Isotype Control (MG1) Summary

• Description: This Mouse IgG1 immunoglobulin is useful as an isotype control for Mouse IgG1 antibody.
• Specificity: In an Ouchterlony double diffusion assay, the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: This isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

This product has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum.

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Mouse IgG1 Isotype Control (MG1)

• IgG1
• Igh-4
• RGD1359539
• VH7183

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG1 Isotype Control (NBP1-97005)(4)

Publications using NBP1-97005

• Morales I, Puertas-Avendano R, Sanchez A Et al. Astrocytes and retrograde degeneration of nigrostriatal dopaminergic neurons in Parkinson's disease: removing axonal debris Translational neurodegeneration Nov 2 2021 [PMID: 34727977] (IHC-FrFl)
• Lim RR, Hainsworth DP, Mohan RR, Chaurasia SS Characterization of a functionally active primary microglial cell culture from the pig retina Exp Eye Res 2019 May 20 [PMID: 31103710] (ICC/IF)
• Siegel D, Dehn DD, Bokatzian SS et al. Redox modulation of NQO1 PLoS ONE 2018 Jan 03 [PMID: 29298345] (Mouse)
• Kusne Y, Carrera-Silva EA, Perry AS et al. Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFalpha in glioblastoma. Sci Signal 2014 Aug 12 [PMID: 25118327] (WB)

Review for IgG1 Isotype Control (NBP1-97005) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 04/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: human fetal derived neural stem cells
• Species: Human
• Lot: 28929

Blocking

• Blocking Details: N/A

Primary Anitbody

• Dilution Ratio: mixed in a 1:50 dilution of 1XPBS for 30 minutes at room temperature

Secondary Antibody

• Secondary Description: goat anti-mouse APC/Cy7
• Secondary Manufacturer Cat#: Abcam, ab130785
• Secondary Concentration: 1:50

Details

• Detection Notes: detected on flow cytometry, RL2 (750nm)
• Fixation Details: sample fixed in PFA for 15 minutes at room temperature
• Permeabilization Details: N/A
• Wash Description: samples were washed with 1XPBS at room temperatue, 3mL per wash

FAQs for IgG1 Isotype Control (NBP1-97005). (Showing 1 - 2 of 2 FAQs).

1. I would like to know the host strain of this product?
   • This isotype control was purified from ascites and consists of antibody from Balb/c with possible trace amounts from Swiss webster.
2. Could you please let me know the supply form (Liquid or Lyohpilized) of this antibody? 
   • Yes, it is liquid. Supplied in 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: none. Preservative: 0.01% Sodium Azide.