HIF-2 alpha/EPAS1 Antibody (ep190b) [PerCP]

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Product Details

Summary
Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
ep190b
Clonality
Monoclonal
Host
Mouse
Conjugate
PerCP

Order Details

HIF-2 alpha/EPAS1 Antibody (ep190b) [PerCP] Summary

Immunogen
Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]
Specificity
This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPAS1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
This HIF-2 alpha (ep190b) antibody is useful for ELISA, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, it recognizes a band at ~118 kDa representing HIF-2 alpha. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Ability to use in mouse is mixed with some positive and some negative results.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HIF-2 alpha/EPAS1 Antibody (ep190b) [PerCP]

  • Basic-helix-loop-helix-PAS protein MOP2
  • BHLHE73
  • bHLHe73ECYT4
  • Class E basic helix-loop-helix protein 73
  • endothelial PAS domain protein 1
  • endothelial PAS domain-containing protein 1
  • EPAS1
  • EPAS-1
  • HIF 2A
  • HIF-1alpha-like factor
  • HIF2 alpha
  • HIF-2 alpha
  • hif2a angiogenesis
  • HIF2A
  • HIF2AHIF-2-alpha
  • HLF
  • HLFHIF-1-alpha-like factor
  • hypoxia-inducible factor 2 alpha
  • Hypoxia-inducible factor 2-alpha
  • Member of PAS protein 2
  • MOP2
  • MOP2HIF2-alpha
  • PASD2PAS domain-containing protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for HIF-2 alpha/EPAS1 Antibody (NB100-132PCP) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NB100-132UV
DyLight 405 Labeled NB100-132V
DyLight 550 Labeled NB100-132R
DyLight 755 Labeled NB100-132IR
HRP Labeled NB100-132H
PE Labeled NB100-132PE

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Bioinformatics Tool for HIF-2 alpha/EPAS1 Antibody (NB100-132PCP)

Discover related pathways, diseases and genes to HIF-2 alpha/EPAS1 Antibody (NB100-132PCP). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for HIF-2 alpha/EPAS1 Antibody (NB100-132PCP)

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Blogs on HIF-2 alpha/EPAS1.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-2 alpha, Tumor Suppression and Cell Survival
HIF-2 alpha is one subunit within the HIF-2 nuclear protein that regulates cellular responses to hypoxia (low oxygen tension conditions). Hydroxylation post-translational modifications on particular HIF residues target them for degradation. Luo, et al...  Read full blog post.

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Bioinformatics

Gene Symbol EPAS1
Entrez