HIF-2 alpha/EPAS1 Products

Antibodies
HIF-2 alpha/EPAS1 Antibody [U ...
HIF-2 alpha/EPAS1 Antibody [Unconj...
AF2997
Species: Hu, Mu, Rt
Applications: WB, IHC
Host: Goat Polyclonal
Formulation Catalog # Availability Price  
Antibody Packs
HIF-2 alpha/EPAS1 Antibody Pa ...
HIF-2 alpha/EPAS1 Antibody Pack
NB100-902HIF2
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO
Host: Mouse
ELISA Kits
Human/Rat Total HIF-2 alpha/E ...
Human/Rat Total HIF-2 alpha/EPAS1 ...
DYC2997-2
Species: Hu, Rt
Applications: ELISA
Conjugate Catalog # Availability Size Price
Formulation Catalog # Availability Price  
Lysates
HIF-2 alpha/EPAS1 Overexpress ...
HIF-2 alpha/EPAS1 Overexpression L...
NBL1-10286
Species: Hu, Mu
Applications: WB
Proteins
Recombinant Human HIF-2 alpha ...
Recombinant Human HIF-2 alpha/EPAS...
H00002034-P03
Species: Hu
Applications: WB, ELISA, PA, AP
Recombinant Human HIF-2 alpha ...
Recombinant Human HIF-2 alpha/EPAS...
H00002034-Q01
Species: Hu
Applications: WB, ELISA, PA, PAGE, AP
HIF-2 alpha/EPAS1 Recombinant ...
HIF-2 alpha/EPAS1 Recombinant Prot...
NBP2-58653PEP
Species: Hu
Applications: AC

Description

Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease (1). In cancer, and particularly solid tumors, hypoxia plays a critical role in the regulation of genes involved in stem cell renewal, epithelial to mesenchymal transition (EMT), metastasis and angiogenesis. In the tumor microenvironment (TME), hypoxia influences the properties and function of stromal cells (e.g., fibroblasts, endothelial and immune cells) and is a strong determinant of tumor progression (2,3).

HIF-1 or hypoxia inducible factor 1, is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. Similarly, HIF-2 alpha plays a role in cellular responses to hypoxia, but whereas HIF-1 alpha is ubiquitously expressed, HIF-2 alpha is predominantly expressed in the vascular endothelium at embryonic stages and after birth in select cells and tissue types (e.g., fibroblasts, hepatocytes and myocytes at 96kDa) (4). Following a similar mechanism to HIF-1 alpha, HIF-2 alpha is stabilized under hypoxic conditions by the formation of a heterodimer with an ARNT/HIF-1 beta subunit. Stable HIF-2 alpha-ARNT/HIF-1 beta heterodimers engage p300/CBP in the nucleus for binding to hypoxic response elements (HREs), inducing transcription, and thus regulation of genes (e.g., EPO, VEGFA). HIF-1 predominantly transactivates genes involved in glycolytic control and pro-apoptotic genes (e.g., LDHA and BNIP3), and HIF-2 regulates the expression of genes involved in invasion and stemness (e.g., MMP2, and OCT4). Common gene targets for HIF-1 and HIF-2 include VEGFA and GLUT1 (5).

The HIF-2 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system under normoxic conditions. This process is mediated by oxygen-sensing enzymes, prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-405 and Pro-531) within the oxygen-dependent degradation domain of HIF-2 alpha (5). Once hydroxylated, HIF-2 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (5,6).

References

1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.

2.Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413

3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y

4. Hu, C.-J., Wang, L.-Y., Chodosh, L. A., Keith, B., & Simon, M. C. (2003). Differential Roles of Hypoxia-Inducible Factor 1 (HIF-1) and HIF-2 in Hypoxic Gene Regulation. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.23.24.9361-9374.2003

5. Koh, M. Y., & Powis, G. (2012). Passing the baton: The HIF switch. Trends in Biochemical Sciences. https://doi.org/10.1016/j.tibs.2012.06.004

6. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483

Bioinformatics

Entrez Human
Mouse
Rat
Uniprot Human
Human
Mouse
Rat
Product By Gene ID 2034
Alternate Names
  • ECYT4
  • hif2a angiogenesis
  • HIF2A
  • HIF-2-alpha
  • HLF
  • HIF-1-alpha-like factor
  • MOP2
  • HIF2-alpha
  • PASD2
  • PAS domain-containing protein 2
  • EPAS1
  • HIF-2 alpha
  • HIF2A
  • BHLHE73
  • Member of PAS protein 2
  • hypoxia-inducible factor 2 alpha
  • Hypoxia-inducible factor 2-alpha
  • HIF-1alpha-like factor
  • Class E basic helix-loop-helix protein 73
  • endothelial PAS domain-containing protein 1
  • Basic-helix-loop-helix-PAS protein MOP2
  • EPAS-1
  • endothelial PAS domain protein 1

Research Areas for HIF-2 alpha/EPAS1

Find related products by research area and learn more about each of the different research areas below.

Angiogenesis
Cancer
Chromatin Research
HIF Target Genes
Hypoxia
Transcription Factors and Regulators

PTMs for HIF-2 alpha/EPAS1

Learn more about PTMs related to HIF-2 alpha/EPAS1.

Phosphorylation
Hydroxylation
Oxidation
Methylation

Bioinformatics Tool for HIF-2 alpha/EPAS1

Discover related pathways, diseases and genes to HIF-2 alpha/EPAS1. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
 
Vizit™, under license from BioVista Inc.

Related HIF-2 alpha/EPAS1 Blog Posts

Check out the latest blog posts on HIF-2 alpha/EPAS1.
HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...    Read more.
HIF-2 alpha, Tumor Suppression and Cell Survival
HIF-2 alpha is one subunit within the HIF-2 nuclear protein that regulates cellular responses to hypoxia (low oxygen tension conditions). Hydroxylation post-translational modifications on particular HIF residues target them for degradation. Luo, et al...    Read more.
Read more HIF-2 alpha/EPAS1 related blogs.