HIF-2 alpha/EPAS1 Antibody (ep190b) [DyLight 650]

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Product Details

Summary
Product Discontinued
View other related HIF-2 alpha/EPAS1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-132C
    • Availability
      Product Discontinued

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HIF-2 alpha/EPAS1 Antibody (ep190b) [DyLight 650] Summary

Immunogen
Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]
Specificity
This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPAS1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This HIF-2 alpha (ep190b) antibody is useful for ELISA, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, it recognizes a band at ~118 kDa representing HIF-2 alpha. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Ability to use in mouse is mixed with some positive and some negative results.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HIF-2 alpha/EPAS1 Antibody (ep190b) [DyLight 650]

  • Basic-helix-loop-helix-PAS protein MOP2
  • BHLHE73
  • bHLHe73ECYT4
  • Class E basic helix-loop-helix protein 73
  • endothelial PAS domain protein 1
  • endothelial PAS domain-containing protein 1
  • EPAS1
  • EPAS-1
  • HIF 2A
  • HIF-1alpha-like factor
  • HIF2 alpha
  • HIF-2 alpha
  • hif2a angiogenesis
  • HIF2A
  • HIF2AHIF-2-alpha
  • HLF
  • HLFHIF-1-alpha-like factor
  • hypoxia-inducible factor 2 alpha
  • Hypoxia-inducible factor 2-alpha
  • Member of PAS protein 2
  • MOP2
  • MOP2HIF2-alpha
  • PASD2PAS domain-containing protein 2

Background

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1 alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HIF-2 alpha/EPAS1 Antibody (NB100-132C). (Showing 1 - 3 of 3 FAQ).

  1. Do you sell the proper isotype control for this antibody? If not, do you know where I can find this? Or, can I use a APC conjugated mouse IgG1 for isotype control?
    • Any APC-conjugated IgG1 isotype control is appropriate. NBP1-43003 is the proper isotype control for this antibody that we carry.
  2. Regarding NB100-132C, the datasheet says "Ability to use in mouse is mixed with some positive and some negative results." Is this due to mouse anti-mouse effect? Or, because this antibody doesn't cross react well with mouse antigen?
    • The mixed results on staining mouse cells comes from customer feedback. This is simply because of the general issues in mouse-on-mouse staining.
  3. Is Dylight 650 similar to APC?
    • Yes, DyLight 650 is an equivalent of APC.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NB100-132UV
DyLight 405 Labeled NB100-132V
DyLight 550 Labeled NB100-132R
DyLight 755 Labeled NB100-132IR
HRP Labeled NB100-132H
PE Labeled NB100-132PE
PerCP Labeled NB100-132PCP

Additional HIF-2 alpha/EPAS1 Products

Bioinformatics Tool for HIF-2 alpha/EPAS1 Antibody (NB100-132C)

Discover related pathways, diseases and genes to HIF-2 alpha/EPAS1 Antibody (NB100-132C). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for HIF-2 alpha/EPAS1 Antibody (NB100-132C)

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Pathways for HIF-2 alpha/EPAS1 Antibody (NB100-132C)

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PTMs for HIF-2 alpha/EPAS1 Antibody (NB100-132C)

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Research Areas for HIF-2 alpha/EPAS1 Antibody (NB100-132C)

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Blogs on HIF-2 alpha/EPAS1.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-2 alpha, Tumor Suppression and Cell Survival
HIF-2 alpha is one subunit within the HIF-2 nuclear protein that regulates cellular responses to hypoxia (low oxygen tension conditions). Hydroxylation post-translational modifications on particular HIF residues target them for degradation. Luo, et al...  Read full blog post.

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Bioinformatics

Gene Symbol EPAS1
Entrez