Goat anti-Mouse IgG (H L) Secondary Antibody [HRP]

Images

 
Western Blot: Goat anti-Mouse, Human IgG (H+L) Secondary Antibody [HRP] [NB7539] - Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539]
Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human ...read more
Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - Goat anti-Mouse IgG Antibody [HRP] [NB7539] - This secondary antibody used for detection of beta-Actin in human pancreatic cancer cell lines. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

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Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG-heavy and light chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:50000
  • ELISA 1:10000-1:1000000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:40 - 1:400
  • Immunohistochemistry-Paraffin 1:40 - 1:400
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB7539 in the following applications:

Publications
Read Publications using
NB7539 in the following applications:

  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.1% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7539)(9)

We have publications tested in 2 confirmed species: Mouse, Parasite.

We have publications tested in 1 application: WB.


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WB
(4)
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Mouse
(3)
Parasite
(1)
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Showing Publications 1 - 9 of 9.
Publications using NB7539 Applications Species
Perlenfein TJ, Murphy RM. A mechanistic model to predict effects of cathepsin B and cystatin C on b-amyloid aggregation and degradation. J. Biol. Chem. 2017 Oct 18 [PMID: 29046353] (WB, Mouse) WB Mouse
Ebneter JA, Heusser SD, Schraner EM et al. Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia. Nat Commun. Dec 15 2016 [PMID: 27976675] (WB, Parasite) WB Parasite
Moghimi E, Solomon JA, Gianforcaro A, Hamadeh MJ. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One 2015 [PMID: 26020962] (WB, Mouse) WB Mouse
Meerson A, Traurig M, Ossowski V et al. Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-alpha. Diabetologia 2013 Jun 12 [PMID: 23756832] (WB, Mouse) WB Mouse
Giessrigl B, Krieger S, Rosner M et al. Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells. Hum Mol Genet 2012 Nov [PMID: 22843495]
Lin YT, Ding JY, Li MY et al. YAP regulates neuronal differentiation through Sonic hedgehog signaling pathway. Exp Cell Res 2012 Sep [PMID: 22659622]
Kim HJ, Lim SJ, Kwag HL et al. The choice of resin-bound ligand affects the structure and immunogenicity of column-purified human papillomavirus type 16 virus-like particles. PLoS One 2012 [PMID: 22563414]
Soenawan E, Srivastava I, Gupta S et al. Maintenance of long-term immunological memory by low avidity IgM-secreting cells in bone marrow after mucosal immunizations with cholera toxin adjuvant. Vaccine 2004 Mar [PMID: 15063581]
Helske S, Vuorela P, Carpen O et al. Expression of vascular endothelial growth factor receptors 1, 2 and 3 in placentas from normal and complicated pregnancies. Mol Hum Reprod 2001 Feb [PMID: 11160848]

Review for IgG (H+L) Antibody (NB7539) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB7539:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NB7539
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reviewed by:
Ming Lu
WB Human 08/14/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman Pancreatic Cancer Cell line
SpeciesHuman
LotA33
CommentsThis secondary antibody works very well, gives clean bands with almost no background. Very happy customer.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Novusbio Cat#NB7539
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives strong band at correct MW.

Comments

CommentsThis secondary antibody works very well, gives clean bands with almost no background. Very happy customer.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7539) (0)

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Recent Reviews

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Ming Lu
08/14/2014
Application: WB
Species: Human