Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP]


Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539]
Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. ...read more
Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - This Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] used for detection of beta-Actin in human pancreatic cancer cell lines. Image from ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
1.0 mg/ml

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Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Summary

This Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] was developed against mouse IgG-heavy and light chain.
By immunoelectrophoresis and ELISA this Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Immunogen affinity purified


  • Western Blot 1:5000-1:50000
  • ELISA 1:10000-1:1000000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:5000
  • Immunohistochemistry 1:200 - 1:5000
  • Immunohistochemistry-Paraffin 1:50 - 1:500
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Reviewed Applications
Read 1 Review rated 5
NB7539 in the following applications:

Read Publications using NB7539.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Phosphate Buffered Saline (PBS) containing 0.2%BSA
0.05% Pro-Clean 400
1.0 mg/ml
Immunogen affinity purified


Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).


1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7539)(15)

We have publications tested in 3 applications: IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB7539 Applications Species
Han Y, Sanford L, Simpson DM et al. Remodeling of Zn(2+) homeostasis upon differentiation of mammary epithelial cells Metallomics Jan 18 2020 [PMID: 31950952] (WB) WB
Verma V, Gupta S, Kumar P et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections Front Microbiol Sep 26 2019 [PMID: 31551961] (WB) WB
Katsumi T, Guicciardi ME, Azad A et al. Activated cholangiocytes release macrophage-polarizing extracellular vesicles bearing the DAMP S100A11 Am. J. Physiol., Cell Physiol. Jul 31 2019 [PMID: 31365294]
Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep Feb 7 2019 [PMID: 30723259] (IHC-P) IHC-P
Lu J, Shen X, Sun X et al. Increased recruitment of endogenous stem cells and chondrogenic differentiation by a composite scaffold containing bone marrow homing peptide for cartilage regeneration Theranostics Nov 16 2018 [PMID: 30429885] (IHC) IHC
Wang X, Hybiske K, Stephens RS. Direct visualization of the expression and localization of chlamydial effector proteins within infected host cells Pathog Dis Feb 2 2018 [PMID: 29390129] (WB) WB
Perlenfein TJ, Murphy RM. A mechanistic model to predict effects of cathepsin B and cystatin C on beta-amyloid aggregation and degradation J Biol Chem Oct 20 2017 [PMID: 29046353] (WB) WB
Ebneter JA, Heusser SD, Schraner EM et al. Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia Nat Commun Dec 16 2016 [PMID: 27976675] (WB) WB
Moghimi E, Solomon JA, Gianforcaro A et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis PLoS One May 29 2015 [PMID: 26020962] (WB) WB
Meerson A, Traurig M, Ossowski V et al. Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-alpha. Diabetologia Jun 13 2013 [PMID: 23756832] (WB) WB
Show All 15 Publications.

Review for IgG (H+L) Antibody (NB7539) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB7539:
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NB7539
reviewed by:
Ming Lu
WB Human 08/14/2014


ApplicationWestern Blot
Sample TestedHuman Pancreatic Cancer Cell line
CommentsThis secondary antibody works very well, gives clean bands with almost no background. Very happy customer.


Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Novusbio Cat#NB7539
Secondary Concentration1:5000


Detection Notes1 min exposure gives strong band at correct MW.


CommentsThis secondary antibody works very well, gives clean bands with almost no background. Very happy customer.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7539). (Showing 1 - 1 of 1 FAQs).

  1. Does NB7539 recognize all mouse IgG subclasses?
    • Yes, this antibody will target all IgG subtypes.

Other Available Formats

Alkaline Phosphatase NB7536
Biotin NB7537
HRP NB7539

Additional IgG (H+L) Products

Array NB7539

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Recent Reviews


Ming Lu
Application: WB
Species: Human