Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP]

Images

 
Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539]
Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. ...read more
Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - This Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] used for detection of beta-Actin in human pancreatic cancer cell lines. Image from ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

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Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
This Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] was developed against mouse IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:50000
  • ELISA 1:10000-1:1000000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:5000
  • Immunohistochemistry 1:200 - 1:5000
  • Immunohistochemistry-Paraffin 1:50 - 1:500
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB7539 in the following applications:

Publications
Read Publications using NB7539.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7539)(15)

We have publications tested in 3 applications: IHC, IHC-P, WB.


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IHC
(1)
IHC-P
(1)
WB
(7)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB7539 Applications Species
Han Y, Sanford L, Simpson DM et al. Remodeling of Zn(2+) homeostasis upon differentiation of mammary epithelial cells Metallomics Jan 18 2020 [PMID: 31950952] (WB) WB
Verma V, Gupta S, Kumar P et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections Front Microbiol Sep 26 2019 [PMID: 31551961] (WB) WB
Katsumi T, Guicciardi ME, Azad A et al. Activated cholangiocytes release macrophage-polarizing extracellular vesicles bearing the DAMP S100A11 Am. J. Physiol., Cell Physiol. Jul 31 2019 [PMID: 31365294]
Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep Feb 7 2019 [PMID: 30723259] (IHC-P) IHC-P
Lu J, Shen X, Sun X et al. Increased recruitment of endogenous stem cells and chondrogenic differentiation by a composite scaffold containing bone marrow homing peptide for cartilage regeneration Theranostics Nov 16 2018 [PMID: 30429885] (IHC) IHC
Wang X, Hybiske K, Stephens RS. Direct visualization of the expression and localization of chlamydial effector proteins within infected host cells Pathog Dis Feb 2 2018 [PMID: 29390129] (WB) WB
Perlenfein TJ, Murphy RM. A mechanistic model to predict effects of cathepsin B and cystatin C on beta-amyloid aggregation and degradation J Biol Chem Oct 20 2017 [PMID: 29046353] (WB) WB
Ebneter JA, Heusser SD, Schraner EM et al. Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia Nat Commun Dec 16 2016 [PMID: 27976675] (WB) WB
Moghimi E, Solomon JA, Gianforcaro A et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis PLoS One May 29 2015 [PMID: 26020962] (WB) WB
Meerson A, Traurig M, Ossowski V et al. Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-alpha. Diabetologia Jun 13 2013 [PMID: 23756832] (WB) WB
Show All 15 Publications.

Review for IgG (H+L) Antibody (NB7539) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB7539:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NB7539
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5
reviewed by:
Ming Lu
WB Human 08/14/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman Pancreatic Cancer Cell line
SpeciesHuman
LotA33
CommentsThis secondary antibody works very well, gives clean bands with almost no background. Very happy customer.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Novusbio Cat#NB7539
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives strong band at correct MW.

Comments

CommentsThis secondary antibody works very well, gives clean bands with almost no background. Very happy customer.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7539). (Showing 1 - 1 of 1 FAQs).

  1. Does NB7539 recognize all mouse IgG subclasses?
    • Yes, this antibody will target all IgG subtypes.

Other Available Formats

Alkaline Phosphatase NB7536
Biotin NB7537
FITC NB7538
HRP NB7539

Additional IgG (H+L) Products

Array NB7539

Research Areas for IgG (H+L) Antibody (NB7539)

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Recent Reviews

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Ming Lu
08/14/2014
Application: WB
Species: Human