Mouse IL-6 Quantikine ELISA Kit, 2 Plate

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Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse IL-6 Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-6. Results obtained using natural mouse IL-6 sho...wed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6.
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Specificity
Natural and recombinant mouse IL-6
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il6

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
M6000B in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-6 Quantikine ELISA Kit, 2 Plate

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor. 

IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6 (M6000B)(388)

We have publications tested in 3 confirmed species: Human, Mouse, Mouseq.

We have publications tested in 1 application: ELISA Capture.


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ELISA Capture
(5)
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Human
(3)
Mouse
(378)
Mouseq
(1)
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Showing Publications 1 - 10 of 388. Show All 388 Publications.
Publications using M6000B Applications Species
H Sun, Y Jiang, Y Song, X Zhang, J Wang, J Zhang, J Kang The MUC5B Mucin Is Involved in Paraquat-Induced Lung Inflammation Oxid Med Cell Longev, 2020;2020(0):7028947. 2020 [PMID: 32724493] (Mouse) Mouse
X Wu, S Pan, W Luo, Z Shen, X Meng, M Xiao, B Tan, K Nie, T Tong, X Wang Roseburia�intestinalis?derived flagellin ameliorates colitis by targeting miR?223?3p?mediated activation of NLRP3 inflammasome and pyroptosis Mol Med Rep, 2020;0(0):. 2020 [PMID: 32700754] (Mouse) Mouse
K Maruyama, H Kidoya, N Takemura, E Sugisawa, O Takeuchi, T Kondo, MMA Eid, H Tanaka, MM Martino, N Takakura, Y Takayama, S Akira, A Vandenbon, Y Kumagai Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages Cell Rep, 2020;32(2):107906. 2020 [PMID: 32668247] (Mouse) Mouse
J Habault, C Fraser, E Pasquereau, M Born-Bony, A Marie-Card, JL Poyet Efficient Therapeutic Delivery by a Novel Cell-Penetrating Peptide Derived from Acinus Cancers (Basel), 2020;12(7):. 2020 [PMID: 32664285] (Mouse) Mouse
P Clua, M Tomokiyo, F Raya Tonet, MA Islam, V García Cas, G Marcial, S Salva, S Alvarez, H Takahashi, S Kurata, H Kitazawa, J Villena The Role of Alveolar Macrophages in the Improved Protection against Respiratory Syncytial Virus and Pneumococcal Superinfection Induced by the Peptidoglycan of Lactobacillus rhamnosus CRL1505 Cells, 2020;9(7):. 2020 [PMID: 32660087] (Mouse) Mouse
JY Kim, SJ Lee, YI Maeng, J Leem, KK Park Protective Effects of Bee Venom against Endotoxemia-Related Acute Kidney Injury in Mice Biology (Basel), 2020;9(7):. 2020 [PMID: 32640615] (Mouse) Mouse
WC Chen, NJ Chen, HP Chen, WK Yu, VY Su, H Chen, HH Wu, KY Yang Nintedanib Reduces Neutrophil Chemotaxis via Activating GRK2 in Bleomycin-Induced Pulmonary Fibrosis Int J Mol Sci, 2020;21(13):. 2020 [PMID: 32630825] (Mouse) Mouse
G de la Rosa, AI Gómez, MC Baños, P Pelegrín Signaling Through Purinergic Receptor P2Y2 Enhances Macrophage IL-1&beta Production Int J Mol Sci, 2020;21(13):. 2020 [PMID: 32630144] (Mouse) Mouse
D Zhao, L Cai, X Lu, X Liang, J Li, P Chen, M Ittmann, X Shang, S Jiang, H Li, C Meng, I Flores, JH Song, JW Horner, Z Lan, CJ Wu, J Li, Q Chang, KC Chen, G Wang, P Deng, DJ Spring, YA Wang, RA DePinho Chromatin Regulator, CHD1, Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer Cancer Discov, 2020;0(0):. 2020 [PMID: 32385075] (Mouse) Mouse
F Sivandzade, F Alqahtani, A Sifat, L Cucullo The cerebrovascular and neurological impact of chronic smoking on post-traumatic brain injury outcome and recovery: an in vivo study J Neuroinflammation, 2020;17(1):133. 2020 [PMID: 32340626] (Mouse) Mouse
Show All 388 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 (M6000B). (Showing 1 - 2 of 2 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
  2. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP M6000B
HRP M6000B
HRP M6000B

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Blogs on IL-6. Showing 1-10 of 12 blog posts - Show all blog posts.


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Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender?
By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm...  Read full blog post.


  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol Il6