The lysate was created in HEK293T cells, using plasmid ID RC208604 and based on accession number NM_001430.
C-terminal Myc/DDK tag
Homo sapiens endothelial PAS domain protein 1 (EPAS1), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug). Has been reported in the literature to react with Mouse (PMID 22308314)
96.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBL1-10286 in the following applications:
Reactivity to mouse reported in publication (PMID: 22308314).
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Array
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for HIF-2 alpha/EPAS1 Overexpression Lysate
Basic-helix-loop-helix-PAS protein MOP2
Class E basic helix-loop-helix protein 73
endothelial PAS domain protein 1
endothelial PAS domain-containing protein 1
hypoxia-inducible factor 2 alpha
Hypoxia-inducible factor 2-alpha
Member of PAS protein 2
PAS domain-containing protein 2
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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