HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP]

Images

 
Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP] [NB100-132H] - Analysis of HIF-2 alpha stabilization over time in HOS cells following exposure to hypoxia. [PMID: 23785417]
Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP] [NB100-132H] - Analysis of HIF-2 alpha stabilization over time in 791T cells following exposure to hypoxia. [PMID: 23785417]

Product Details

Summary
Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
ep190b
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Order Details

HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP] Summary

Immunogen
Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]
Specificity
This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPAS1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This HIF-2 alpha (ep190b) antibody is useful for ELISA, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, it recognizes a band at ~118 kDa representing HIF-2 alpha. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
Positive Control
HIF-2 alpha/EPAS1 Lysate (NBL1-10286)
Publications
Read Publications using
NB100-132H in the following applications:

  • IHC
    1 publication

Reactivity Notes

Ability to use in mouse is mixed with some positive and some negative results.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP]

  • Basic-helix-loop-helix-PAS protein MOP2
  • BHLHE73
  • bHLHe73ECYT4
  • Class E basic helix-loop-helix protein 73
  • endothelial PAS domain protein 1
  • endothelial PAS domain-containing protein 1
  • EPAS1
  • EPAS-1
  • HIF 2A
  • HIF-1alpha-like factor
  • HIF2 alpha
  • HIF-2 alpha
  • hif2a angiogenesis
  • HIF2A
  • HIF2AHIF-2-alpha
  • HLF
  • HLFHIF-1-alpha-like factor
  • hypoxia-inducible factor 2 alpha
  • Hypoxia-inducible factor 2-alpha
  • Member of PAS protein 2
  • MOP2
  • MOP2HIF2-alpha
  • PASD2PAS domain-containing protein 2

Background

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1 alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIF-2 alpha/EPAS1 Antibody (NB100-132H)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HIF-2 alpha/EPAS1 Antibody (NB100-132H) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NB100-132UV
DyLight 405 Labeled NB100-132V
DyLight 550 Labeled NB100-132R
DyLight 755 Labeled NB100-132IR
PE Labeled NB100-132PE
PerCP Labeled NB100-132PCP

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Bioinformatics Tool for HIF-2 alpha/EPAS1 Antibody (NB100-132H)

Discover related pathways, diseases and genes to HIF-2 alpha/EPAS1 Antibody (NB100-132H). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on HIF-2 alpha/EPAS1.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-2 alpha, Tumor Suppression and Cell Survival
HIF-2 alpha is one subunit within the HIF-2 nuclear protein that regulates cellular responses to hypoxia (low oxygen tension conditions). Hydroxylation post-translational modifications on particular HIF residues target them for degradation. Luo, et al...  Read full blog post.

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Bioinformatics

Gene Symbol EPAS1
Entrez