VEGFR1/Flt-1 Antibody

Images

 
VEGF R1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3 µg/mL overnight ...read more
VEGF R1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

VEGFR1/Flt-1 Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1
Ser27-His687
Accession # NP_001153392
Specificity
Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant mouse VEGF R1 and less than 10% cross-reactivity with recombinant human (rh) VEGF R2 and rhVEGF R3.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FLT1
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
Positive Control
VEGFR1/Flt-1 Lysate (NBP2-66318)
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF321 in the following applications:

Publications
Read Publications using
AF321 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR1/Flt-1 Antibody (AF321)(24)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 11 applications: Blocking, Flow, Functional Assay, ICC, IHC, IHC Frozen, IHC Paraffin-embedded, IHC-P, Neut, Proximity Ligation Assay, WB.


Filter By Application
Blocking
(1)
Flow
(1)
Functional Assay
(1)
ICC
(4)
IHC
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(1)
IHC-P
(1)
Neut
(8)
Proximity Ligation Assay
(1)
WB
(4)
All Applications
Filter By Species
Human
(23)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF321 Applications Species
M Spiel, S Salahuddin, E Pernicone, Z Zsengeller, A Wang, AM Modest, SA Karumanchi, JL Hecht Placental soluble fms-like tyrosine kinase expression in small for gestational age infants and risk for adverse outcomes Placenta, 2017;52(0):10-16. 2017 [PMID: 28454690] (IHC-P,, Human) IHC-P Human
HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson Ferrochelatase is a therapeutic target for ocular neovascularization EMBO Mol Med, 2017;0(0):. 2017 [PMID: 28377496] (WB,, Human) WB Human
Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration. PLoS ONE, 2015;10(9):e0138587. 2015 [PMID: 26381529] (Neut,, Human) Neut Human
Kim H, Kim K, Kim B, Cho M, Lee S The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis. PLoS ONE, 2015;10(4):e0124909. 2015 [PMID: 25894998] (Neut,, Human) Neut Human
Szalai G, Romero R, Chaiworapongsa T, Xu Y, Wang B, Ahn H, Xu Z, Chiang P, Sundell B, Wang R, Jiang Y, Plazyo O, Olive M, Tarca A, Dong Z, Qureshi F, Papp Z, Hassan S, Hernandez-Andrade E, Than N Full-length human placental sFlt-1-e15a isoform induces distinct maternal phenotypes of preeclampsia in mice. PLoS ONE, 2015;10(4):e0119547. 2015 [PMID: 25860260] (WB,, Human) WB Human
Lezcano C, Kleffel S, Lee N, Larson A, Zhan Q, DoRosario A, Wang L, Schatton T, Murphy G Merkel cell carcinoma expresses vasculogenic mimicry: demonstration in patients and experimental manipulation in xenografts. Lab Invest, 2014;94(10):1092-102. 2014 [PMID: 25111691] (IHC Frozen,, Human) IHC Frozen Human
Hou H, Cheng S, Chung K, Kuo M, Yeh C, Chang B, Lu H, Wang H, Yu C Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis. Autophagy, 2014;10(9):1509-21. 2014 [PMID: 24988221] (Neut,, Human) Neut Human
Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling. Mol Ther, 2014;22(7):1243-53. 2014 [PMID: 24769910] (Functional Assay,, Human) Functional Assay Human
Vizio, Barbara, Biasi, Fiorella, Scirelli, Tiziana, Novarino, Anna, Prati, Adriana, Ciuffreda, Libero, Montrucchio, Giuseppe, Poli, Giuseppe, Bellone, Graziell Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors. J Transl Med, 2013;11(0):314. 2013 [PMID: 24341512] (Blocking,, Human) Blocking Human
Larson A, Lee C, Lezcano C, Zhan Q, Huang J, Fischer A, Murphy G Melanoma spheroid formation involves laminin-associated vasculogenic mimicry. Am J Pathol, 2014;184(1):71-8. 2014 [PMID: 24332013] (IHC,, Human) IHC Human
Show All 24 Publications.

Reviews for VEGFR1/Flt-1 Antibody (AF321) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF321:
Filter by Applications
Flow
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 01/07/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 19757485
SpeciesHuman

Blocking

Blocking DetailsSee PMID 19757485

Details

Detection NotesSee PMID 19757485

Comments

CommentsPublished: See PMID 19757485Independent review from antibodyresource.comFITC-conjugated anti-Flt-1 antibody was produced be reacting the affinity purified goat anti-hVEGF R1 (Flt-1) immmunoglobulin G antibody (R&D Systems AF321) with a four-fold molar excess of 6[fluorescein-5-(-and-6)-carboxamido]-hexanoic acid succinimidyl ester (Molecular Probes) for 1 hour at 37 oC, followed by purification by size-exclusion chromatography on a Nick-25 mini-column.
 
reviewed by:
Anonymous
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21699503
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21699503

Details

Detection NotesSee PMID 21699503

Comments

CommentsPublished: See PMID 21699503Independent review from antibodyresource.com

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FAQs for VEGFR1/Flt-1 Antibody (AF321) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Diseases for VEGFR1/Flt-1 Antibody (AF321)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
01/07/2015
Application: Flow
Species: Human

 
Anonymous
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol FLT1
Entrez
Uniprot