VEGFR1/Flt-1 Antibody [Unconjugated]

Images

 
VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 ...read more
VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3 µg/mL overnight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

VEGFR1/Flt-1 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
Ser27-His687
Accession # NP_001153392
Specificity
Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FLT1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF321 in the following applications:

Publications
Read Publications using
AF321 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR1/Flt-1 Antibody (AF321)(38)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 10 applications: Blocking, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
Blocking
(1)
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(5)
IHC
(4)
IHC-Fr
(2)
IHC-P
(2)
Neutralization
(9)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(12)
All Applications
Filter By Species
Human
(36)
Bovine
(2)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using AF321 Applications Species
J Chen, Y Wang, S Wang, X Zhao, L Zhao, Y Wang Salvianolic acid B and ferulic acid synergistically promote angiogenesis in HUVECs and zebrafish via regulating VEGF signaling Journal of ethnopharmacology, 2021;0(0):114667. 2021 [PMID: 34597652] (Western Blot, Human) Western Blot Human
V Aleksandro, A Wrona, T Bereza, K Pity?ski, K Gil Oviductal Telocytes in Patients with Uterine Myoma Biomedicines, 2021;9(8):. 2021 [PMID: 34440264] (IHC, Human) IHC Human
S Ichimura, K Ohara, M Kono, K Mizutani, Y Kitamura, I Saga, R Kanai, T Akiyama, M Toda, M Kohno, K Yoshida, H Sasaki Molecular investigation of brain tumors progressing during pregnancy or postpartum period: the association between tumor type, their receptors, and the timing of presentation Clinical neurology and neurosurgery, 2021;207(0):106720. 2021 [PMID: 34102420] (IHC, Human) IHC Human
, 0;0(0):. 0 [PMID: 33348564] (Western Blot, Human) Western Blot Human
D Dong, Y Fu, F Chen, J Zhang, H Jia, J Li, H Wang, J Wen Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF?1&amp;alpha and VEGF expression Mol Med Rep, 2021;23(1):. 2021 [PMID: 33215223] (Neutralization, Human) Neutralization Human
ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling Cell Death Dis, 2020;11(5):307. 2020 [PMID: 32366833] (Western Blot, Human) Western Blot Human
C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée BMP9 signaling promotes the normalization of tumor blood vessels Oncogene, 2020;0(0):. 2020 [PMID: 32042114] (Western Blot, Human) Western Blot Human
ZK Zsengellér, A Lo, M Tavasoli, E Pernicone, SA Karumanchi, S Rosen Soluble fms-Like Tyrosine Kinase 1 Localization in Renal Biopsies of CKD Kidney Int Rep, 2019;4(12):1735-1741. 2019 [PMID: 31844810] (IHC-Fr, Human) IHC-Fr Human
J Cao, R Yang, TE Smith, S Evans, GW McCollum, SC Pomerantz, T Petley, IR Harris, JS Penn Human Umbilical Tissue-Derived Cells Secrete Soluble VEGFR1 and Inhibit Choroidal Neovascularization Mol Ther Methods Clin Dev, 2019;14(0):37-46. 2019 [PMID: 31276010] (Western Blot, Human) Western Blot Human
KY Han, JH Chang, DT Azar MMP14-Containing Exosomes Cleave VEGFR1 and Promote VEGFA-Induced Migration and Proliferation of Vascular Endothelial Cells Invest. Ophthalmol. Vis. Sci., 2019;60(6):2321-2329. 2019 [PMID: 31117124] (ICC, Human) ICC Human
Show All 38 Publications.

Reviews for VEGFR1/Flt-1 Antibody (AF321) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF321:
Filter by Applications
Flow Cytometry
(1)
Western Blot
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
Flow Cytometry Human 01/07/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 19757485
SpeciesHuman
  4
reviewed by:
Anonymous -
Western Blot Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21699503
SpeciesHuman

Product General Protocols

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FAQs for VEGFR1/Flt-1 Antibody (AF321) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for VEGFR1/Flt-1 Antibody (AF321)

Discover related pathways, diseases and genes to VEGFR1/Flt-1 Antibody (AF321). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for VEGFR1/Flt-1 Antibody (AF321)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous -
01/07/2015
Application: Flow Cytometry
Species: Human

 
Anonymous -
01/07/2015
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol FLT1
Uniprot