VEGFR1/Flt-1 Antibody [Unconjugated]

VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VEGFR1/Flt-1 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
  Ser27-His687
  Accession # NP_001153392
• Specificity: Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FLT1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody [Unconjugated]

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR1/Flt-1 Antibody (AF321)(43)

Publications using AF321

• X Huang, L Jia, Y Jia, X Xu, R Wang, M Wei, H Li, H Peng, Y Wei, Q He, K Wang sFlt-1-enriched exosomes induced endothelial cell dysfunction and a preeclampsia-like phenotype in mice Cytokine, 2023-04-14;166(0):156190. 2023-04-14 [PMID: 37062152] (Western Blot, Human)
• X Liu, Z Li, J Sun, Z Zhang, W Li Interaction between PD-L1 and soluble VEGFR1 in glioblastoma-educated macrophages BMC Cancer, 2023-03-20;23(1):259. 2023-03-20 [PMID: 36941554] (Functional Assay, Human)
• F Zakeri, H Latifi-Nav, ZS Soheili, M Sadeghi, SS Arab, S Samiei, ER Pirmardan, S Taghizadeh, H Ahmadieh, A Hafezi-Mog Design, construction and in vivo functional assessment of a hinge truncated sFLT01 Gene Therapy, 2022-09-16;0(0):. 2022-09-16 [PMID: 36114375] (Western Blot, Human)
• A Wrona, V Aleksandro, T Bereza, P Basta, A Gil, M Ulatowska-, M Mazur-Lask, K Pity?ski, K Gil Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes International Journal of Molecular Sciences, 2022-05-31;23(11):. 2022-05-31 [PMID: 35682833] (IHC, Human)
• DN Mitroi, M Tian, R Kawaguchi, WE Lowry, ST Carmichael Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia Journal of Cellular and Molecular Medicine, 2022-05-11;0(0):. 2022-05-11 [PMID: 35543222] (IHC, Human)
• J Chen, Y Wang, S Wang, X Zhao, L Zhao, Y Wang Salvianolic acid B and ferulic acid synergistically promote angiogenesis in HUVECs and zebrafish via regulating VEGF signaling Journal of ethnopharmacology, 2021-09-29;0(0):114667. 2021-09-29 [PMID: 34597652] (Western Blot, Human)
• V Aleksandro, A Wrona, T Bereza, K Pity?ski, K Gil Oviductal Telocytes in Patients with Uterine Myoma Biomedicines, 2021-08-20;9(8):. 2021-08-20 [PMID: 34440264] (IHC, Human)
• S Ichimura, K Ohara, M Kono, K Mizutani, Y Kitamura, I Saga, R Kanai, T Akiyama, M Toda, M Kohno, K Yoshida, H Sasaki Molecular investigation of brain tumors progressing during pregnancy or postpartum period: the association between tumor type, their receptors, and the timing of presentation Clinical neurology and neurosurgery, 2021-06-01;207(0):106720. 2021-06-01 [PMID: 34102420] (IHC, Human)
• 2020-12-17;0(0):. 2020-12-17 [PMID: 33348564] (Western Blot, Human)
• D Dong, Y Fu, F Chen, J Zhang, H Jia, J Li, H Wang, J Wen Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF?1&amp;alpha and VEGF expression Mol Med Rep, 2020-11-20;23(1):. 2020-11-20 [PMID: 33215223] (Neutralization, Human)
• ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling Cell Death Dis, 2020-05-04;11(5):307. 2020-05-04 [PMID: 32366833] (Western Blot, Human)
• C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée BMP9 signaling promotes the normalization of tumor blood vessels Oncogene, 2020-02-10;0(0):. 2020-02-10 [PMID: 32042114] (Western Blot, Human)
• ZK Zsengellér, A Lo, M Tavasoli, E Pernicone, SA Karumanchi, S Rosen Soluble fms-Like Tyrosine Kinase 1 Localization in Renal Biopsies of CKD Kidney Int Rep, 2019-08-14;4(12):1735-1741. 2019-08-14 [PMID: 31844810] (IHC-Fr, IHC-P, Human)
• J Cao, R Yang, TE Smith, S Evans, GW McCollum, SC Pomerantz, T Petley, IR Harris, JS Penn Human Umbilical Tissue-Derived Cells Secrete Soluble VEGFR1 and Inhibit Choroidal Neovascularization Mol Ther Methods Clin Dev, 2019-05-22;14(0):37-46. 2019-05-22 [PMID: 31276010] (Western Blot, Human)
• KY Han, JH Chang, DT Azar MMP14-Containing Exosomes Cleave VEGFR1 and Promote VEGFA-Induced Migration and Proliferation of Vascular Endothelial Cells Invest. Ophthalmol. Vis. Sci., 2019-05-01;60(6):2321-2329. 2019-05-01 [PMID: 31117124] (ICC, Human)
• SHS Lee, HJ Kim, OK Shin, JS Choi, J Kim, YH Cho, J Ha, TK Park, JY Lee, K Park, H Lee Intravitreal Injection of AAV Expressing Soluble VEGF Receptor-1 Variant Induces Anti-VEGF Activity and Suppresses Choroidal Neovascularization Invest. Ophthalmol. Vis. Sci., 2018-11-01;59(13):5398-5407. 2018-11-01 [PMID: 30452593] (Western Blot, Human)
• S Capossela, A Bertolo, K Gunasekera, T Pötzel, M Baur, JV Stoyanov VEGF vascularization pathway in human intervertebral disc does not change during the disc degeneration process BMC Res Notes, 2018-05-22;11(1):333. 2018-05-22 [PMID: 29784013] (Western Blot, Bovine, Human)
• SK Gonzales, M Badell, H Cottrell, B Rimawi, V Deepak, N Sidell, A Rajakumar Villous explants from preeclamptic placentas induce sFlt1 in PBMCs: An ex vivo co-culture study Pregnancy Hypertens, 2018-02-25;12(0):40-46. 2018-02-25 [PMID: 29674197] (Western Blot, Human)
• M Spiel, S Salahuddin, E Pernicone, Z Zsengeller, A Wang, AM Modest, SA Karumanchi, JL Hecht Placental soluble fms-like tyrosine kinase expression in small for gestational age infants and risk for adverse outcomes Placenta, 2017-02-10;52(0):10-16. 2017-02-10 [PMID: 28454690] (IHC-P, Human)
• HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson Ferrochelatase is a therapeutic target for ocular neovascularization EMBO Mol Med, 2017-06-01;0(0):. 2017-06-01 [PMID: 28377496] (Western Blot, Human)
• Alessio D'alessio, Gabriella Proietti, Gina Lama, Filippo Biamonte, Libero Lauriola, Umberto Moscato, Angelo Vescovi, Annunziato Mangiola, Cristiana Angelucci, Gigliola Sica Analysis of angiogenesis related factors in glioblastoma, peritumoral tissue and their derived cancer stem cells. Oncotarget, 2016-11-29;0(0):1949-2553. 2016-11-29 [PMID: 27705944] (IHC, Human)
• Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration. PLoS ONE, 2015-09-18;10(9):e0138587. 2015-09-18 [PMID: 26381529] (Neutralization, Human)
• Kim H, Kim K, Kim B, Cho M, Lee S The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis. PLoS ONE, 2015-04-20;10(4):e0124909. 2015-04-20 [PMID: 25894998] (Neutralization, Human)
• Szalai G, Romero R, Chaiworapongsa T, Xu Y, Wang B, Ahn H, Xu Z, Chiang P, Sundell B, Wang R, Jiang Y, Plazyo O, Olive M, Tarca A, Dong Z, Qureshi F, Papp Z, Hassan S, Hernandez-Andrade E, Than N Full-length human placental sFlt-1-e15a isoform induces distinct maternal phenotypes of preeclampsia in mice. PLoS ONE, 2015-04-10;10(4):e0119547. 2015-04-10 [PMID: 25860260] (Western Blot, Human)
• Lezcano C, Kleffel S, Lee N, Larson A, Zhan Q, DoRosario A, Wang L, Schatton T, Murphy G Merkel cell carcinoma expresses vasculogenic mimicry: demonstration in patients and experimental manipulation in xenografts. Lab Invest, 2014-08-11;94(10):1092-102. 2014-08-11 [PMID: 25111691] (IHC-Fr, Human)
• Hou H, Cheng S, Chung K, Kuo M, Yeh C, Chang B, Lu H, Wang H, Yu C Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis. Autophagy, 2014-06-25;10(9):1509-21. 2014-06-25 [PMID: 24988221] (Neutralization, Human)
• Shvartsman , Dmitry, Storrie-White , Hannah, Lee , Kangwon, Kearney , Cathal, Brudno , Yevgeny, Ho , Nhi, Cezar , Christin, McCann , Corey, Anderson , Erin, Koullias , John, Tapia , Juan Car, Vandenburgh , Herman, Lichtman , Jeff W, Mooney , David J Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling. Mol Ther, 2014-04-28;22(7):1243-53. 2014-04-28 [PMID: 24769910] (Functional Assay, Human)
• Vizio , Barbara, Biasi , Fiorella, Scirelli , Tiziana, Novarino , Anna, Prati , Adriana, Ciuffreda , Libero, Montrucchio , Giuseppe, Poli , Giuseppe, Bellone , Graziell Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors. J Transl Med, 2013-12-17;11(0):314. 2013-12-17 [PMID: 24341512] (Blocking, Human)
• Larson A, Lee C, Lezcano C, Zhan Q, Huang J, Fischer A, Murphy G Melanoma spheroid formation involves laminin-associated vasculogenic mimicry. Am J Pathol, 2014-01-01;184(1):71-8. 2014-01-01 [PMID: 24332013] (IHC, Human)
• Nevo , Ori, Lee , Dennis K, Caniggia , Isabella Attenuation of VEGFR-2 expression by sFlt-1 and low oxygen in human placenta. PLoS ONE, 2013-11-19;8(11):e81176. 2013-11-19 [PMID: 24260556] (Neutralization, Western Blot, Human)
• Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noel A, Goffin F, Tsatsaris V, Subtil D, Foidart JM Differential expression of Vegfr-2 and its soluble form in preeclampsia. PLoS ONE, 2012-03-12;7(3):e33475. 2012-03-12 [PMID: 22428059] (IHC-P, Western Blot, Human)
• Cai J, Chen Z, Ruan Q, Han S, Liu L, Qi X, Boye SL, Hauswirth WW, Grant MB, Boulton ME gamma-Secretase and presenilin mediate cleavage and phosphorylation of vascular endothelial growth factor receptor-1. J. Biol. Chem., 2011-10-20;286(49):42514-23. 2011-10-20 [PMID: 22016384] (Proximity Ligation Assay (PLA), Bovine)
• Barsoum IB, Renaud SJ, Graham CH Glyceryl trinitrate inhibits hypoxia-induced release of soluble fms-like tyrosine kinase-1 and endoglin from placental tissues. Am. J. Pathol., 2011-06-01;178(6):2888-96. 2011-06-01 [PMID: 21641407] (IHC-P, Human)
• Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt. Invest. Ophthalmol. Vis. Sci., 2009-10-15;51(2):1190-7. 2009-10-15 [PMID: 19834034] (ICC, Neutralization, Human)
• Lee HS, Myers A, Kim J Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis. Am. J. Respir. Crit. Care Med., 2009-09-17;180(11):1056-67. 2009-09-17 [PMID: 19762561] (IHC, Neutralization, Human)
• Ball SG, Shuttleworth CA, Kielty CM Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J. Cell Biol., 2007-04-30;177(3):489-500. 2007-04-30 [PMID: 17470632] (Neutralization, Human)
• Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Am. J. Pathol., 2006-10-01;169(4):1167-82. 2006-10-01 [PMID: 17003476] (Neutralization, Human)
• Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro. Chem. Biol., 2006-05-01;13(5):549-56. 2006-05-01 [PMID: 16720276] (Flow Cytometry, Human)
• Akintoye SO, Lam T, Shi S, Brahim J, Collins MT, Robey PG Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals. Bone, 2006-01-03;38(6):758-68. 2006-01-03 [PMID: 16403496] (ICC, Human)
• Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung. Proc. Natl. Acad. Sci. U.S.A., 2005-07-11;102(29):10212-7. 2005-07-11 [PMID: 16009933] (ICC, Neutralization, Human)
• Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis. Exp. Cell Res., 2004-09-10;299(1):68-78. 2004-09-10 [PMID: 15302574] (Neutralization, Human)
• Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells. Leuk. Res., 2004-03-01;28(3):243-8. 2004-03-01 [PMID: 14687619] (ICC, Human)
• Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis. Blood, 2003-03-20;102(2):638-45. 2003-03-20 [PMID: 12649156] (Neutralization, Human)

Reviews for VEGFR1/Flt-1 Antibody (AF321) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous - Flow Human 01/07/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 19757485
• Species: Human

reviewed by: Anonymous - WB Human 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21699503
• Species: Human

Bioinformatics

• Gene Symbol: FLT1
• Uniprot:
  • NP_001153392()