EGFR Antibody [Unconjugated]

Images

 
EGFR was detected in immersion fixed frozen sections of human skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 1 hour at room temperature ...read more
EGFR was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 3 hours at room ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen ...read more
A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231, filled histogram) or isotype control antibody (Catalog # AB-108-C, ...read more
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 4.2 mg/mL. A specific band was detected for EGFR at approximately 229 kDa (as indicated) using 12.5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

EGFR Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human EGFR
Leu25-Ser645
Accession # CAA25240
Specificity
Detects human EGFR in ELISAs and Western blots. In sandwich ELISAs, approximately 3% cross-reactivity with recombinant mouse EGFR is observed and less than 0.1% cross-reactivity with recombinant human (rh) ErbB2 and rhErbB3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EGFR
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunoprecipitation 1 ug/mL
  • Simple Western 12.5 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human EGF R/ErbB1 Antibody (Catalog # AF231)
ELISA Detection:Human EGF R/ErbB1 Biotinylated Antibody (Catalog # BAF231)
Standard: Recombinant Human EGF R/ErbB1 (Catalog # 1095-ER)
Publications
Read Publications using
AF231 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGFR Antibody [Unconjugated]

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC 2.7.10.1
  • EGF R
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1

Background

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha , betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha  in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis (1 - 3).

  1. Daly, R.J. (1999) Growth Factors, 16:255.
  2. Schlessinger, J. (2000) Cell. 103:211.
  3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGFR Antibody (AF231)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: Co-Immunoprecipitation, ELISA Detection, ELISA Development, ELISA Microarray Development, IF, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Co-Immunoprecipitation
(1)
ELISA Detection
(1)
ELISA Development
(1)
ELISA Microarray Development
(1)
IF
(1)
IHC
(2)
IHC-P
(2)
Immunoprecipitation
(2)
Western Blot
(4)
All Applications
Filter By Species
Human
(14)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF231 Applications Species
DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression The Journal of Biological Chemistry, 2022;0(0):102029. 2022 [PMID: 35569509] (Immunoprecipitation, Human) Immunoprecipitation Human
C Hu, CA Leche, A Kiyatkin, Z Yu, SE Stayrook, KM Ferguson, MA Lemmon Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias Nature, 2022;602(7897):518-522. 2022 [PMID: 35140400] (Western Blot, Human) Western Blot Human
T Egnuni, N Ingram, I Mirza, PL Coletta, JR McLaughlan Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window Pharmaceutics, 2021;13(10):. 2021 [PMID: 34683944] (IF, Human) IF Human
R Chakrabort, K Vickery, C Darido, S Ranganatha, H Hu Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences, 2021;22(16):. 2021 [PMID: 34445392] (Western Blot, Human) Western Blot Human
X Liu, V Adorno-Cru, YF Chang, Y Jia, M Kawaguchi, NK Dashzeveg, R Taftaf, EK Ramos, EJ Schuster, L El-Shennaw, D Patel, Y Zhang, M Cristofani, H Liu EGFR inhibition blocks cancer stem cell clustering and lung metastasis of triple negative breast cancer Theranostics, 2021;11(13):6632-6643. 2021 [PMID: 33995681] (Co-Immunoprecipitation, Human) Co-Immunoprecipitation Human
S Sugita, M Yamato, T Hatabu, Y Kataoka Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment Scientific Reports, 2021;11(1):9668. 2021 [PMID: 33994540] (IHC, Mouse) IHC Mouse
N Yogo, T Hase, T Kasama, K Nishiyama, N Ozawa, T Hatta, H Shibata, M Sato, K Komeda, N Kawabe, K Matsuoka, TF Chen-Yoshi, N Kaji, M Tokeshi, Y Baba, Y Hasegawa Development of an immuno-wall device for the rapid and sensitive detection of EGFR mutations in tumor tissues resected from lung cancer patients PLoS ONE, 2020;15(11):e0241422. 2020 [PMID: 33196648] (ELISA Detection, Human) ELISA Detection Human
F Haun, S Neumann, L Peintner, K Wieland, J Habicht, C Schwan, K Østevold, MM Koczorowsk, M Biniossek, M Kist, H Busch, M Boerries, RJ Davis, U Maurer, O Schilling, K Aktories, C Borner Identification of a novel anoikis signalling pathway using the fungal virulence factor gliotoxin Nat Commun, 2018;9(1):3524. 2018 [PMID: 30166526] (Western Blot, Human) Western Blot Human
A Klaesson, K Grannas, T Ebai, J Heldin, B Koos, M Leino, D Raykova, J Oelrich, L Arngården, O Söderberg, U Landegren Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes Sci Rep, 2018;8(1):5400. 2018 [PMID: 29599435] (IHC-P, Human) IHC-P Human
Suzanne D Burke Soluble fms-like tyrosine kinase 1 promotes angiotensin II sensitivity in preeclampsia J Clin Invest, 2016;0(0):. 2016 [PMID: 27270170] (IHC-P, Human) IHC-P Human
Show All 15 Publications.

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The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

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Bioinformatics

Gene Symbol EGFR
Uniprot