Von Hippel Lindau Antibody

Images

 
Western Blot: Von Hippel Lindau Antibody [NB100-41384] - (0.01ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Von Hippel Lindau Antibody [NB100-41384] - (5ug/ml) staining of Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA: Von Hippel Lindau Antibody [NB100-41384] - (1.5ug/ml) as the reporter with VHL antibody as the capture rabbit antibody (2.5ug/ml).

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, PEP-ELISA, S-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Von Hippel Lindau Antibody Summary

Immunogen
Peptide with sequence C-RSLVKPENYRRLD corresponding to internal region according to NP_937799.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VHL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • ELISA 1.5-3 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Sandwich ELISA 1.5 - 3 ug/ml
Application Notes
EIA: Sandwich ELISA with increasing amount of recombinant VHL captured by a rabbit antibody.
WB: Approx. 17 kDa band observed in human ovary testis lysates, mouse/rat testis and brain lysates, and all human/mouse cell lines (calculated MW of 19.7 kDa band according to NP_937799.1).
IHC: Paraffin embedded Human Kidney.
Control
Von Hippel Lindau Overexpression Lysate
Publications
Read Publication using NB100-41384.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Von Hippel Lindau Antibody

  • elongin binding protein
  • HRCA1
  • Protein G7
  • pVHL
  • RCA1
  • VHL
  • VHL1
  • von Hippel-Lindau disease tumor suppressor
  • von Hippel-Lindau syndrome
  • von Hippel-Lindau tumor suppressor

Background

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Von Hippel Lindau Antibody (NB100-41384)(1)

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol VHL
Entrez
Uniprot