HIF-2 alpha/EPAS1 Antibody (ep190b) [PE]

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Product Details

Summary
Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications Flow
Clone
ep190b
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

HIF-2 alpha/EPAS1 Antibody (ep190b) [PE] Summary

Immunogen
Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]
Specificity
This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPAS1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
This HIF-2 alpha (ep190b) antibody is useful for ELISA, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, it recognizes a band at ~118 kDa representing HIF-2 alpha. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Ability to use in mouse is mixed with some positive and some negative results.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HIF-2 alpha/EPAS1 Antibody (ep190b) [PE]

  • Basic-helix-loop-helix-PAS protein MOP2
  • BHLHE73
  • bHLHe73ECYT4
  • Class E basic helix-loop-helix protein 73
  • endothelial PAS domain protein 1
  • endothelial PAS domain-containing protein 1
  • EPAS1
  • EPAS-1
  • HIF 2A
  • HIF-1alpha-like factor
  • HIF2 alpha
  • HIF-2 alpha
  • hif2a angiogenesis
  • HIF2A
  • HIF2AHIF-2-alpha
  • HLF
  • HLFHIF-1-alpha-like factor
  • hypoxia-inducible factor 2 alpha
  • Hypoxia-inducible factor 2-alpha
  • Member of PAS protein 2
  • MOP2
  • MOP2HIF2-alpha
  • PASD2PAS domain-containing protein 2

Background

Hypoxia-inducible factors (HIFs) generates heterodimers composed of alpha-subunit (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunit (Arnt1, Arnt2 and Arnt3). Of these isoforms, HIF-1 alpha and HIF-2 alpha play a critical role in the cellular response to hypoxic conditions. After heterodimerization with beta subunits and binding to DNA sequence 5'-[AG]CGTG-3' within HRE (hypoxia response element) of target gene promoters, HIF-2 alpha regulates the gene expression in response to oxygen homeostasis alterations. HIF-2 alpha regulates VEGF expression for involving in the development of new blood vessels as well as the tubular system of lung. HIF-2 alpha play a role in the formation of the endothelium that gives rise to blood brain barrier. HIF-2 alpha is a strong activator of expression of Tie-2 tyrosine kinase which is a major player in angiogenesis. HIF-2alpha activation require recruitment of transcriptional coactivators such as CREBPB, EP300 etc. and its interaction with redox regulatory protein APEX mediates activation of CTAD. HIF-2 alpha/HIF-1alpha expression increases in certain malignancies, wherein, HIF-1 alpha plays both pro- as well as anti-proliferative activities, whereas, HIF-2 alpha is suggested to exert anti-proliferative activity only.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NB100-132UV
DyLight 405 Labeled NB100-132V
DyLight 550 Labeled NB100-132R
DyLight 755 Labeled NB100-132IR
HRP Labeled NB100-132H
PerCP Labeled NB100-132PCP

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Blogs on HIF-2 alpha/EPAS1.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-2 alpha, Tumor Suppression and Cell Survival
HIF-2 alpha is one subunit within the HIF-2 nuclear protein that regulates cellular responses to hypoxia (low oxygen tension conditions). Hydroxylation post-translational modifications on particular HIF residues target them for degradation. Luo, et al...  Read full blog post.

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Bioinformatics

Gene Symbol EPAS1
Entrez