HIF-1 alpha Antibody


Western Blot: HIF-1 alpha Antibody [NBP1-02160] - Detection of HIF-1 alpha using NBP1-02160. Lane 1: COS7 CoCl treated cells Lane 2: COS7 untreated cells.
Simple Western: HIF-1 alpha Antibody [NBP1-02160] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Reactivity Hu, Mu, Po, Bovine, Rabbit, MonkeySpecies Glossary
Applications WB, Simple Western
0.2 mg/ml

HIF-1 alpha Antibody Summary

Genomic sequence made to an internal portion of human HIF-1 alpha (within amino acids 400 - 550) [Swiss-Prot# Q16665].
Predicted Species
Monkey (100%), Bovine (96%), Rabbit (94%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, and 50% Glycerol
0.05% Sodium Azide
0.2 mg/ml
Immunogen affinity purified


  • Western Blot 1:3500
  • Simple Western 1:250
Application Notes
In WB, the bands are seen ~97 kDa and 120 kDa. In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 3.5
NBP1-02160 in the following applications:

Read Publications using
NBP1-02160 in the following applications:

  • WB
    3 publications

Reactivity Notes

Immunogen sequence has 89% homology to Chicken. Mouse and Porcine reactivities were reported by a customer review.

Alternate Names for HIF-1 alpha Antibody

  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • BHLHE78
  • Class E basic helix-loop-helix protein 78
  • HIF1 alpha
  • HIF-1 alpha
  • HIF1A
  • HIF-1-alpha
  • HIF1-alpha
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor)
  • hypoxia-inducible factor 1-alpha
  • Member of PAS protein 1
  • member of PAS superfamily 1
  • PAS domain-containing protein 8
  • PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)


HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VHL (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIF-1 alpha Antibody (NBP1-02160)(4)

Reviews for HIF-1 alpha Antibody (NBP1-02160) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 review tested in 1 species: Mouse.
We have 2 review tested in 1 application: WB.

Reviews using NBP1-02160:
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Images Ratings Applications Species Date Details
Western Blot HIF-1 alpha NBP1-02160
reviewed by:
WB 01/31/2013


ApplicationWestern Blot
Special ApplicationsTested in mouse intestine, Porcine (brain, liver and intestine). Human cell lines as CacO-2, IMR32 and HUVEC
Pub Med ID23203207
reviewed by:
WB Mouse 03/20/2009


ApplicationWestern Blot
Sample TestedMouse Heart Tissue, Sample Amount: 100 ug


Blocking DetailsBlocking Buffer: 5% milk, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/3000, Primary Ab Incubation Dilution Buffer: 5% BSA, Incubation Time: 16 hr, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey mAb, Secondary Ab Dilution Ratio: 1/3000


Detection NotesExposure Time: 1 hour, Non-Specific Bands: 64-98 kDa

Product General Protocols

View specific protocols for HIF-1 alpha Antibody (NBP1-02160): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HIF-1 alpha Antibody (NBP1-02160). (Showing 1 - 5 of 5 FAQs).

  1. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
    • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
  2. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
    • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, as desired by you, I am suggesting you four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products has been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449 ) (cited in at least 31 peer reviewed publications).
  3. I would like to know, does it exist path for detection HIF 1 in the venous blood before and after revascularization of the leg?
    • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
  4. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
    • The theoretical molecular weight of HIF 1-alpha is ~93kDa, however, you will likely see a band between 100-120kDa due to phosphorylation.
  5. What do you mean with HIF-1 alpha unprocessed?
    • The unprocessed refers to the unregulated undegraded full length protein that is detected with treatment, but not post translationally modified and phosphorylated. The PTM protein is expressed at the higher molecular weight.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-02160AF405
Alexa Fluor (R) 488 Labeled NBP1-02160AF488
Alexa Fluor (R) 647 Labeled NBP1-02160AF647
Alexa Fluor (R) 700 Labeled NBP1-02160AF700
Biotin Labeled NBP1-02160B
DyLight 488 Labeled NBP1-02160G
DyLight 550 Labeled NBP1-02160R
DyLight 650 Labeled NBP1-02160C
HRP Labeled NBP1-02160H

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Blogs on HIF-1 alpha. Showing 1-10 of 25 blog posts - Show all blog posts.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Controls for HIF-1 Alpha's WB, ICC-IF, IHC, IP & FLOW Analysis
Tips on positive and negative controls for HIF-1 alpha antibodies is one of the most Frequently Asked Questions on Hypoxia and HIFs. Here are top 5 suggestions from Novus Biologicals: The degradation of HIF1 alpha is the most common issue whic...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

Comprehensive Autophagy Research Tools - New Catalog Available Now!
Autophagy, a protein degradation process through autophagosome-lysosomal pathway, is important for cellular homeostasis and plays a role in many diseases. To help researchers learn more about this process and the products available for its study, N...  Read full blog post.

HIF-1 beta: A dimerization partner of HIF-1 alpha required for an adaptive response to hypoxia
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia-indu...  Read full blog post.

HIF-1 Alpha: Infographic
Encoded by the HIF1A gene, HIF-1 alpha has a critical role in cellular response to hypoxia. In hypoxic conditions, HIF-1 alpha activates the transcription of several genes to facilitate metabolic reaction for lack of oxygen. In normoxic conditions, HI...  Read full blog post.

Controlling the HIF-1 Switch
Hypoxia-inducible factor-1 is a major transcription factor composed of two subunits: HIF-1alpha and HIF-1 beta. Under normoxic conditions, HIF-1 alpha is targeted to proteosomal degradation via ubiquitination. On the other hand during hypoxic conditio...  Read full blog post.

Showing 1-10 of 25 blog posts - Show all blog posts.

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Gene Symbol HIF1A

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