Immunocytochemistry/ Immunofluorescence: LC3B Antibody [NB600-1384] - Confocal analysis of HeLa cells using LC3B antibody (NB600-1384, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunocytochemistry/ Immunofluorescence: LC3B Antibody [NB600-1384] - LC3B/MAP1 [NB600-1384] - LC3 antibody was tested in 50uM Chloroquine treated HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: LC3B Antibody [NB600-1384] - LC3B/MAP1 [NB600-1384] - Analysis using the HRP conjugate of NB600-1384. Staining of treated U373-MG cells using ...read more
Western Blot: LC3B Antibody [NB600-1384] - Analysis of LC3B in IB3-1 whole cell lysate using anti-LC3B antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: LC3B Antibody [NB600-1384] - Analysis of LC3B in HeLa cells using anti-LC3B antibody (red). Nuclei were counterstained with DAPI (blue).
Biological Strategies: Western Blot: LC3B Antibody [NB600-1384] - LC3B/MAP1 [NB600-1384] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates.
Western Blot: LC3B Antibody [NB600-1384] - Analysis in HEK 293T cell lysate. Verified customer review.
Immunocytochemistry/ Immunofluorescence: LC3B Antibody [NB600-1384] - Untreated HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Biological Strategies: Western Blot: LC3B Antibody [NB600-1384] - Aldosterone (ALD) treated SV-K1 cell. Western blot shows lysates of SV-K1 cell line untreated (-) or treated with aldosterone. This image was ...read more
Immunocytochemistry/ Immunofluorescence: LC3B Antibody [NB600-1384] - HeLa cells were treated with 50uM CQ overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% ...read more
Immunohistochemistry-Paraffin: LC3B Antibody [NB600-1384] - Analysis of U87MG glioma xenografts using anti-LC3B antibdoy. Image from verified customer review.
Simple Western: LC3B Antibody [NB600-1384] - Lane view shows a specific band for LC3B in 0.5 mg/ml of Neuro2A lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
In Western Blot, bands are seen at ~17 and 19 kDa corresponding to LC3-II and LC3-I. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Electron Microscopy was reported in scientific literature (PMID: 21885071).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Zebrafish reactivity reported in scientific literature (PMID: 23724125). Canine and primate reactivity reported in scientific literature (PMID: 24027311) Porcine reactivity reported in scientific literature (PMID: 25378587) Other species have not been tested.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for LC3B Antibody
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
lc3b autophagy marker
MAP1 light chain 3-like protein 2
MAP1A/MAP1B LC3 B
microtubule associated protein 3 b
microtubule associated protein 3 beta
microtubule-associated protein 1 light chain 3 betaMAP1A/MAP1B light chain 3 B
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Yu Y, Xie Y, Cao L et al. The Ferroptosis Inducer Erastin Enhances Sensitivity of Acute Myeloid Leukemia Cells to Chemotherapeutic Agents Molecular & Cellular Oncology. 2015 May 26 (WB, Human)
Schlafli AM, Berezowska S, Adams O, Langer R. Reliable LC3 and p62 autophagy marker detection in formalin fixed paraffin embedded human tissue by immunohistochemistry. Euro Jrnl of Histochemistry. 2015 (IHC-P, Human)
IF Confocal analysis of HeLa cells using LC3B antibody (NB600-1384, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FAQs for LC3B Antibody (NB600-1384). (Showing 1 - 4 of 4 FAQs).
What is the immunogen sequence of this antibody?
The exact immunogen sequence is proprietary, however we can tell you that the immunogen sequence can be found between amino acids 1-50 of human LC3B.
Does this antibody also recognize mouse LC3A?
This antibody does not cross-react with LC3A. If you are interested in detecting mouse LC3A and LC3B, you may want to consider purchasing our LC3/LC3B antibody pack, NB910-40752.
Is it possible to provide me the suggested dilution of Electron Microscopy and IHC of NB600-1384?
Electron microscopy was performed by a customer, so unfortunately, we do not have their dilution information. For IHC, we recommend a dilution of 1:400.
What % gel used for WB to obtain the separation of the LC3BI and II bands?
We prefer to use a 4-20% gradient gel to get ample separation between the LC3BI and II bands. If you are using a single percentage gel, a 12% or higher gel should separate the lower weights enough for proper detection.
The LC3 A, B, C’s and 1, 2, 3’s By Christina Towers, PhD Autophagy is a catabolic process used to breakdown and recycle damaged proteins and organelles. It is a multistep process that, in its simplest form, consists of 4 steps: initiation, phagophor... Read full blog post.
Animal Models to Study Autophagy By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. In the beginning... the process of macroautophagy was originally characterized in... Read full blog post.
Epigenetic Control of Autophagy By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studied.... Read full blog post.
LC3B: From Autophagy to Cancer LC3B is subunit component of the LC3 autophagy biomarker associated with microtubule-associated proteins MAP1A and MAP1B and one of the best characterized markers to date. In resting state it is cytosolic, but upon activation, is lapidated and becomes... Read full blog post.
Analyzing LC3 in Western blot Microtubule-associated protein light chain 3 (LC3) is considered one of the definitive markers of autophagy, and its use is widespread in labs throughout the world. Despite its popularity, there are several considerations when employing LC3 antibodies... Read full blog post.
Marking the Autophagosome: the LC3 Antibody MAP1LC3 (shortened to LC3 in our antibody catalog) is one of four mammalian homologues of autophagy-related protein 8 (Atg8). It has been identified as a light chain subunit of the microtubule-associated proteins MAP1A/MAP1B. A modified form of LC3, L... Read full blog post.
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