LC3B Antibody

Images

 
Biological Strategies: Western Blot: LC3B Antibody [NB600-1384] - Analysis of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using anti- [Catalog # NB600-1384].
Biological Strategies: Immunohistochemistry: LC3B Antibody [NB600-1384] - Staining of treated U373-MG (human glioblastoma) cells using anti- [Catalog # NB600-1384].
Biological Strategies: Immunocytochemistry/ Immunofluorescence: LC3B Antibody [NB600-1384] - Immunocytochemical/Immunofluorescent staining of treated U373-MG cells using the HRP conjugate of anti- (Catalog # ...read more
Biological Strategies: Western Blot: LC3B Antibody [NB600-1384] - Depletion of RARalpha prevents ATRA-induced autophagic flux in SKBR3 cells. LC3B western blotting and quantification of the autophagic activity in ...read more
Immunohistochemistry-Paraffin: LC3B Antibody [NB600-1384] - Analysis of U87MG glioma xenografts using anti- [Catalog # NB600-1384]. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: LC3B Antibody [NB600-1384] - Confocal analysis of HeLa cells using Rabbit anti-LC3B antibody (Catalog # NB600-1384, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was ...read more
Electron Microscopy: LC3B Antibody [NB600-1384] - NOD1-dependent induction of autophagy contributes to IRGM and LC3 recruitment to Mtb-containing vesicles. The subcellular localization of autophagy proteins was observed ...read more
Immunohistochemistry: LC3B Antibody [NB600-1384] - LC3B staining in gliobastoma multiform tissue.
Knockdown Validated: LC3B Antibody [NB600-1384] - LC3A and LC3B siRNAs specifically block the expression of the LC3A and LC3B proteins, respectively, in A549 cell line (E1,2,3). In E4 the reactivity of and of the LC3B ...read more
Western Blot: LC3B Antibody [NB600-1384] - Human cervical carcinoma (HeLa) and Mouse Neuroblast cells (Neuro2a) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared ...read more
Immunocytochemistry/ Immunofluorescence: LC3B Antibody [NB600-1384] - Analysis of LC3B in HeLa cells using anti-LC3B antibody (red) [Catalog # NB600-1384]. Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: LC3B Antibody [NB600-1384] - LC3B staining in treated U87-MG cultured & subcutaneous tumors.
Simple Western: LC3B Antibody [NB600-1384] - Lane view shows a specific band for LC3B in 0.5 mg/ml of Neuro2A lysate at a molecular weight of approximately 15 kDa. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ba, Bv, Ca, Pm, ZeSpecies Glossary
Applications WB, Simple Western, ELISA, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

LC3B Antibody Summary

Immunogen
Polyclonal LC3B Antibody was made to a synthetic peptide made to the N-terminal region of the human LC3B protein. [Uniprot: Q9GZQ8]
Localization
LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
Marker
Autophagosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP1LC3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Electron Microscopy
  • ELISA
  • Flow Cytometry 1:200
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 0.1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:400
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Immunoprecipitation
  • Immunohistochemistry Free-Floating
  • Knockdown Validated
  • Knockout Validated
Application Notes
In Western Blot, bands are seen at ~17 and 19 kDa corresponding to LC3-II and LC3-I. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Electron Microscopy was reported in scientific literature (PMID: 21885071). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 24928515). Use in Immunohistochemistry on both paraffin-embedded and frozen sections reported in scientific literature (PMID: 18259115). Use in immunoprecipitation reported in scientific literature (PMID: 26098573). Use in immunoblotting reported in scientific literature (PMID: 25383539). Use in flow reported in scientific literature (PMID: 27622036). Use in ELISA reported in scientific literature (PMID: 21885071). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
14.688 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Brain Hippocampus Membrane Tissue Lysate (Adult Membrane Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Neuro2a Whole Cell Lysate
Neuro2a Chloroquine Treated / Untreated Cell Lysate
HeLa Chloroquine Treated / Untreated Cell Lysate
Reviewed Applications
Read 18 Reviews rated 4.2
using
NB600-1384 in the following applications:

Publications
Read Publications using
NB600-1384 in the following applications:

  • EM
    2 publications
  • 4 publications
  • 4 publications
  • IB
    1 publication
  • 48 publications
  • IHC
    12 publications
  • 2 publications
  • 1 publication
  • 7 publications
  • IP
    1 publication
  • KD
    1 publication
  • WB
    183 publications

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 23724125). Canine and primate reactivity reported in scientific literature (PMID: 24027311) Porcine reactivity reported in scientific literature (PMID: 25378587). Rat reactivity reported in scientific literature (30067379). Bacteria reactivity reported in scientific literature (31110360). Bovine reactivity reported in scientific literature (21868124). Other species have not been tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LC3B Antibody

  • Apg8b
  • ATG8F
  • Autophagy-related protein LC3 B
  • Autophagy-related ubiquitin-like modifier LC3 B
  • lc3b autophagy marker
  • LC3B
  • lc3-i, ii
  • LC3II
  • MAP1 light chain 3-like protein 2
  • MAP1A/1BLC3
  • MAP1A/MAP1B LC3 B
  • MAP1LC3B
  • MAP1LC3B-a
  • microtubule associated protein 1 light chain 3 beta
  • microtubule associated protein 3 b
  • microtubule associated protein 3 beta
  • microtubule-associated proteins 1A/1B light chain 3
  • microtubule-associated proteins 1A/1B light chain 3B

Background

Autophagy (macroautophagy) is a catabolic process which targets intracellular components such as proteins and organelles for degradation. Originally described as a bulk degradation process, current research supports its selective nature (1). Selective autophagy targets specific cellular components for degradation including the endoplasmic reticulum (2) (ER-phagy), mitochondria (3) (mitophagy), peroxisomes (3) (pexophagy), ribosomes (4) (ribophagy) and bacteria (5) (xenophagy). Autophagy relies on a newly formed phagophore, a membrane structure which elongates, sequesters cellular content, and fuses to form a double membrane vesicle known as the autophagosome. Fusion of autophagosomes with lysosomes gives rise to the autophagolysosome, where cellular components are degraded by lysosome hydrolases (1).

Autophagic flux is supported by autophagy-related proteins (Atgs) initially identified in yeast (6,7). The core autophagy machinery is comprised of 17 Atg proteins that play specific roles in autophagosome formation. Among these Atg proteins, Atg8 is not only involved in autophagosome formation but also functions in cargo selection. In mammals, several Atg8 homologues have been identified including microtubule-associated protein 1 light chain 3 alpha, beta and gamma - LC3A, LC3B, and LC3C (8) respectively, as well as GABA type A receptor-associated protein (GABARAP), GABARAP-Like1, and GABARAP-Like2 (9). LC3 (predicted molecular weight 14kD) is ubiquitously expressed and undergoes posttranslational processing after synthesis. First, the cysteine protease Atg4 cleaves a carboxy terminal sequence to generate the cytosolic form LC3-I. Next, E1-like (Atg7) and E2-like (Atg3) enzymes conjugate phosphatidylethanolamine to the newly exposed carboxyterminal glycine, generating LC3-II. Finally, the Atg12-Atg5-Atg16L1 complex participates in LC3 lipidation and autophagosome formation (10). LC3B-I to LC3B-II conversion correlates with autophagosome number and is considered the best marker to monitor autophagy.

References

1. Yu, L., Chen, Y., & Tooze, S. A. (2018). Autophagy pathway: Cellular and molecular mechanisms. Autophagy. https://doi.org/10.1080/15548627.2017.1378838

2. Forrester, A., De Leonibus, C., Grumati, P., Fasana, E., Piemontese, M., Staiano, L.,... Settembre, C. (2019). A selective ER -phagy exerts procollagen quality control via a Calnexin- FAM 134B complex. The EMBO Journal. https://doi.org/10.15252/embj.201899847

3. He, X., Zhu, Y., Zhang, Y., Geng, Y., Gong, J., Geng, J.,... Zhong, H. (2019). RNF34 functions in immunity and selective mitophagy by targeting MAVS for autophagic degradation. The EMBO Journal. https://doi.org/10.15252/embj.2018100978

4. Mathai, B., Meijer, A., & Simonsen, A. (2017). Studying Autophagy in Zebrafish. Cells. https://doi.org/10.3390/cells6030021

5. Losier, T. T., Akuma, M., McKee-Muir, O. C., LeBlond, N. D., Suk, Y., Alsaadi, R. M.,... Russell, R. C. (2019). AMPK Promotes Xenophagy through Priming of Autophagic Kinases upon Detection of Bacterial Outer Membrane Vesicles. Cell Reports. https://doi.org/10.1016/j.celrep.2019.01.062

6. Nakatogawa, H., Suzuki, K., Kamada, Y., & Ohsumi, Y. (2009). Dynamics and diversity in autophagy mechanisms: Lessons from yeast. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm2708

7. Tsukada, M., & Ohsumi, Y. (1993). Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Letters. https://doi.org/10.1016/0014-5793(93)80398-E

8. Wild, P., McEwan, D. G., & Dikic, I. (2014). The LC3 interactome at a glance. Journal of Cell Science. https://doi.org/10.1242/jcs.140426

9. Igloi, G. L. (2001). Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABAA receptor-associated protein. Genomics. https://doi.org/10.1006/geno.2001.6555

10. Glick, D., Barth, S., & Macleod, K. F. (2010). Autophagy: Cellular and molecular mechanisms. Journal of Pathology. https://doi.org/10.1002/path.2697

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-1384
Species: Hu, Mu, Rt, Po, Ba, Bv, Ca, Pm, Ze
Applications: WB, Simple Western, ELISA, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, KD, KO

Publications for LC3B Antibody (NB600-1384)(292)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Bacteria, Bovine, Canine, Porcine, Primate, Zebrafish.

We have publications tested in 12 applications: EM, FLOW, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, IP, KD, WB.


Filter By Application
EM
(2)
FLOW
(4)
Flow
(4)
IB
(1)
ICC/IF
(48)
IHC
(12)
IHC-Fr
(2)
IHC-FrFl
(1)
IHC-P
(7)
IP
(1)
KD
(1)
WB
(183)
All Applications
Filter By Species
Human
(130)
Mouse
(73)
Rat
(13)
Bacteria
(1)
Bovine
(1)
Canine
(3)
Porcine
(2)
Primate
(1)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 292. Show All 292 Publications.
Publications using NB600-1384 Applications Species
Omary Z Understanding the role of LRBA in patients with inflammatory bowel disease Thesis
Chen L, Xia YF, Shen SF et al. Syntaxin 17 inhibits ischemic neuronal injury by resuming autophagy flux and ameliorating endoplasmic reticulum stress Free Radic. Biol. Med. Aug 20 2020 [PMID: 32828953] (WB, KD, Mouse) WB, KD Mouse
Bernard M, Cardin GB, Cahuzac M et al. Dual Inhibition of Autophagy and PI3K/AKT/MTOR Pathway as a Therapeutic Strategy in Head and Neck Squamous Cell Carcinoma Cancers (Basel) Aug 21 2020 [PMID: 32825725] (WB, Human) WB Human
Zhou K, Zheng Z, Li Y et al. TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress Theranostics Jul 23 2020 [PMID: 32802192] (Mouse) Mouse
Zhou X, Brooks M, Jiang P et al. Loss of Tmem106b exacerbates FTLD pathologies and causes motor deficits in progranulin-deficient mice EMBO Rep. Aug 5 2020 [PMID: 32761777]
Li Z, Zhu YT, Xiang M et al. Enhanced lysosomal function is critical for paclitaxel resistance in cancer cells: reversed by artesunate Acta Pharmacol. Sin. Jul 23 2020 [PMID: 32704040] (WB, Human) WB Human
Zhang Z, Quan Q, Mark Li et al. The unfolded protein response regulates hepatic autophagy by sXBP1-mediated activation of TFEB Autophagy 2020 July 15 [PMID: 32597296] (WB, Mouse) WB Mouse
Zhang Z, Quan Q, Mark Li et al. The unfolded protein response regulates hepatic autophagy by sXBP1-mediated activation of TFEB 2020 July 15 [PMID: 32597296] (WB, Mouse) WB Mouse
Cheung CHY, Hsu CL, Lin TY et al. ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer J. Biomed. Sci. Jun 23 2020 [PMID: 32576196] (WB, Human) WB Human
Deynoux M, Sunter N, Ducrocq E et al. A comparative study of the capacity of mesenchymal stromal cell lines to form spheroids PLoS ONE Jun 2 2020 [PMID: 32484831] (IHC, Mouse) IHC Mouse
Show All 292 Publications.

Reviews for LC3B Antibody (NB600-1384) (18) 4.218

Average Rating: 4.2
(Based on 18 reviews)
We have 18 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB600-1384:
Filter by Applications
WB
(12)
IF
(3)
ICC
(1)
IHC
(1)
All Applications
Filter by Species
Human
(7)
Mouse
(8)
Rat
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot LC3B NB600-1384
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reviewed by:
Anonymous
WB Mouse 07/17/2019
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Summary

ApplicationWestern Blot
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
LotCF-1

Comments

CommentsSecondary antibody used: Alexa Fluor 488
Western Blot LC3B NB600-1384
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reviewed by:
Anonymous
WB Human 06/04/2018
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Summary

ApplicationWestern Blot
Sample TestedHek 293T cell lysate
SpeciesHuman
LotCC-1
Western Blot LC3B NB600-1384
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reviewed by:
Bo Ding
WB Mouse 02/20/2018
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Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
SpeciesMouse
LotCJ-1
Western Blot LC3B NB600-1384
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reviewed by:
Anonymous
WB Mouse 07/22/2016
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesMouse
LotBQ

Blocking

Blocking Details5% skim milk in TBS

Primary Anitbody

Dilution Ratio1:1,000

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Concentration0.4 ug/ml

Details

Detection NotesECL, exposure 30 seconds
Western Blot LC3B NB600-1384
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reviewed by:
Anonymous
WB Mouse 06/28/2016
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Summary

ApplicationWestern Blot
Sample Testedmice primary macrophages
SpeciesMouse
LotBO

Blocking

Blocking Details5% milk for 1 hour at room temperature

Primary Anitbody

Dilution Ratio4 degree overnight, 1:1,000 dilution

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP
Secondary Concentration0.4 ug/ml

Details

Detection Noteswith ECL solution, 25 secs of exposure time.
 LC3B NB600-1384
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reviewed by:
Anonymous
Human 02/04/2015
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Summary

SpeciesHuman
FileView PDF
 
reviewed by:
Anonymous
IF Mouse 12/23/2014
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23525089
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23525089

Details

Detection NotesSee PMID 23525089
Fixation DetailsMethanol fixation required.

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23525089.
 
reviewed by:
Anonymous
IF Rat 12/23/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23688351
SpeciesRat

Blocking

Blocking DetailsSee PMID 23688351

Details

Detection NotesSee PMID 23688351

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23688351.
Western Blot LC3B NB600-1384
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reviewed by:
Anonymous
WB Human 11/25/2014
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Summary

ApplicationWestern Blot
Sample TestedIB3-1 whole cell lysate
SpeciesHuman
Lottrial sample

Blocking

Blocking Details3% non-fat dry milk (Bio-Rad) in TBST, 30 min at room temperature

Primary Anitbody

Dilution Ratio1:1000 dilution in blocking buffer, overnight at 4 degree

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#Cell signaling, 7074S
Secondary Concentration1:5000 dilution

Details

Detection Notesfilm detection, using Clarity Western ECL (Bio-Rad) for 3 min.
Western Blot LC3B NB600-1384
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reviewed by:
Anonymous
WB Mouse 11/23/2014
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Summary

ApplicationWestern Blot
Sample Testedmouse hearts
SpeciesMouse

Blocking

Blocking DetailsBSA

Primary Anitbody

Dilution Ratio1:2000

Secondary Antibody

Secondary Descriptionrabbit anti-mouse IgG, HRP
Secondary Concentration1:10000

Details

Detection Notesexposure time 1 min
Western Blot LC3B NB600-1384
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reviewed by:
Anonymous
WB Rat 11/01/2014
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Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
CommentsWorks best with milk, detect 2 nice bands.

Blocking

Blocking DetailsMilk 10%in PBST for 2 hrs at RT

Primary Anitbody

Dilution Ratio1/2000 for 6 hrs at 4 degrees

Secondary Antibody

Secondary DescriptionDonkey anti Rabbit HRP
Secondary Manufacturer Cat#Pierce
Secondary Concentration1/10000

Details

Detection NotesECL with biorad chemidoc XRS

Comments

CommentsWorks best with milk, detect 2 nice bands.
 
reviewed by:
Anonymous
WB Mouse 10/03/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate alveolar macrophages
SpeciesMouse

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG HRP
Western Blot LC3B NB600-1384
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reviewed by:
Mohammad Abedi
WB Human 07/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedNeuroblastoma whole cell lysate
SpeciesHuman
Lot1384

Blocking

Blocking Details5% milk solution overnight at 4oC

Primary Anitbody

Dilution Ratio1:1000, 1 hour Incubation at RT in TBST

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG- HRP Conjugated
Secondary Manufacturer Cat##7074S
Secondary Concentration1:1000 in TBST for 1 hour at RT

Details

Detection NotesDensitometry, VersaDoc MP 5000, 300 seconds
Western Blot LC3B NB600-1384
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reviewed by:
Anony- mous
WB Human 06/10/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa and HT1080 cells using LC3B antibody (NB600-1384, 1:1000).
FileView PDF
Immunocytochemistry LC3B NB600-1384
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reviewed by:
Anony- mous
ICC Human 06/10/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of HeLa cells using LC3B antibody (NB600-1384, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
 
reviewed by:
Anonymous
IHC Human 10/19/2010
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman Esophagus tissue
SpeciesHuman

Details

Fixation DetailsSample Preparation: FFPE
 
reviewed by:
Anonymous
IF Other 08/28/2009
View

Summary

ApplicationImmunofluorescence
Sample TestedHuman Oral Keratinocyte
SpeciesOther
LotK
CommentsIt may be that the LC3 protein is difficult to detect in autophagosomes in Keratinocytes.

Not yet tested in HeLa.

Blocking

Blocking DetailsBlocking Buffer: BSA, Blocking Time: 1 hour, Blocking Temp: 22 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: 2% BSA, Primary Ab Dilution Ratio: 1/2000, Incubation Time: 16hr, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Molecular Probes, Secondary Ab Dilution: 1/200
Secondary Manufacturer Cat#A-11034

Comments

CommentsIt may be that the LC3 protein is difficult to detect in autophagosomes in Keratinocytes.

Not yet tested in HeLa.
 
reviewed by:
Anonymous
WB Mouse 05/26/2009
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver tissue, Sample Amount: 100
SpeciesMouse
LotI1

Blocking

Blocking DetailsBlocking Buffer: 5% milk/PBS, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/2000, Incubation Dilution Buffer: milk/PBS, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat-anti rabbit-HRP (Pierce), Secondary Ab Dilution Ratio: 1/2000

Details

Detection NotesDetection Method: ECL-x-ray, Exposure Time: 1 minute, Specific Bands: 15 kDa, Non-Specific Bands: 20 kDa, 37 kDa, 82 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LC3B Antibody (NB600-1384). (Showing 1 - 5 of 5 FAQs).

  1. Hello, somewhere on the LC3B antibody page I read it is recommended to use a 0.2uM membrane for western blot?
    • Yes, because LC3B-I is about 14-15 kDa and LC3B-II is even even smaller, the protein may slip through the 0.45 uM membrane and be lost during blotting. However, there has been success using 0.45 uM membranes; a 0.2 uM membrane is something to consider if no signal is detected.
  2. What is the immunogen sequence of this antibody?
    • The exact immunogen sequence is proprietary, however we can tell you that the immunogen sequence can be found between amino acids 1-50 of human LC3B.
  3. Does this antibody also recognize mouse LC3A?
    • This antibody does not cross-react with LC3A. If you are interested in detecting mouse LC3A and LC3B, you may want to consider purchasing our LC3/LC3B antibody pack, NB910-40752.
  4. Is it possible to provide me the suggested dilution of Electron Microscopy and IHC of NB600-1384?
    • Electron microscopy was performed by a customer, so unfortunately, we do not have their dilution information. For IHC, we recommend a dilution of 1:400.
  5. What % gel used for WB to obtain the separation of the LC3BI and II bands?
    • We prefer to use a 4-20% gradient gel to get ample separation between the LC3BI and II bands. If you are using a single percentage gel, a 12% or higher gel should separate the lower weights enough for proper detection.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-1384AF350
Alexa Fluor 405 NB600-1384AF405
Alexa Fluor 488 NB600-1384AF488
Alexa Fluor 532 NB600-1384AF532
Alexa Fluor 594 NB600-1384AF594
Alexa Fluor 647 NB600-1384AF647
Alexa Fluor 700 NB600-1384AF700
Alexa Fluor 750 NB600-1384AF750
Biotin NB600-1384B
DyLight 350 NB600-1384UV
DyLight 405 NB600-1384V
DyLight 488 NB600-1384G
DyLight 550 NB600-1384R
DyLight 594 NB600-1384DL594
DyLight 650 NB600-1384C
DyLight 680 NB600-1384FR
DyLight 755 NB600-1384IR
FITC NB600-1384F
HRP NB600-1384H
Janelia Fluor 549 NB600-1384JF549
Janelia Fluor 646 NB600-1384JF646
PE NB600-1384PE

Additional LC3B Products

Array NB600-1384

Bioinformatics Tool for LC3B Antibody (NB600-1384)

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Blogs on LC3B. Showing 1-10 of 24 blog posts - Show all blog posts.

Autophagy and Metastasis
  By Christina Towers, PhD The majority of cancer patients die from metastatic disease at secondary sites. The threshold to undergo metastasis is high. Only a minority of cancer cells acquire invasive phenotypes...  Read full blog post.


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Recent Reviews

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Anonymous
07/17/2019
Application: WB
Species: Mouse

Anonymous
06/04/2018
Application: WB
Species: Human

Bo Ding
02/20/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MAP1LC3B