LC3B Antibody

Western Blot: LC3B Antibody [NB600-1384] - Analysis of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using anti- [Catalog # NB600-1384].

Immunohistochemistry: LC3B Antibody [NB600-1384] - Staining of treated U373-MG (human glioblastoma) cells using anti- [Catalog # NB600-1384].

Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - Immunocytochemical/Immunofluorescent staining of treated U373-MG cells using the HRP conjugate of anti- (Catalog # NB600-1384). The nuclei were stained with DAPI.

Western Blot: LC3B Antibody [NB600-1384] - Depletion of RARalpha prevents ATRA-induced autophagic flux in SKBR3 cells. LC3B western blotting and quantification of the autophagic activity in control and RARalpha-knockdown SKBR3 cells upon treatment with 1 uM ATRA for 2 days in the presence or absence of BafA for 2 h at 200 nM. LC3B-II expression was normalized to GAPDH and to vehicle-treated control cells. Standard deviations from at least five independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2015236), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: LC3B Antibody [NB600-1384] - Analysis of U87MG glioma xenografts using anti- [Catalog # NB600-1384]. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - Confocal analysis of HeLa cells using Rabbit anti-LC3B antibody (Catalog # NB600-1384, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Electron Microscopy: LC3B Antibody [NB600-1384] - NOD1-dependent induction of autophagy contributes to IRGM and LC3 recruitment to Mtb-containing vesicles. The subcellular localization of autophagy proteins was observed in untreated (A, C) and Tri-DAP treated cells (B, D) using anti-IRGM and anti-LC3 antibodies, which were detected using a secondary antibody coupled to 5 nm gold particles (indicated with arrowheads; TEM X 62,000). Gold particles colocalizing with bacteria were manually counted in 10 macrophages of each condition (E, F) and the differences between the treatments are indicated: ***p < 0.01 and *p < 0.05 vs. Mtb, ++p < 0.01 vs. Tri-DAP, Wilcoxon Rank test. Box plots indicate median and quartiles. Image collected and cropped by CiteAb from the following publication (//bmcpulmmed.biomedcentral.com/articles/10.1186/1471-2466-14-152), licensed under a CC-BY licence.

Immunohistochemistry: LC3B Antibody [NB600-1384] - LC3B staining in gliobastoma multiform tissue.

Knockdown Validated: LC3B Antibody [NB600-1384] - LC3A and LC3B siRNAs specifically block the expression of the LC3A and LC3B proteins, respectively, in A549 cell line (E1,2,3). In E4 the reactivity of and of the LC3B (5F10 antibody) is shown, following silencing of the LC3A or of the LC3B genes, in the A549 cell line. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0137675), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - Analysis of LC3B in HeLa cells using anti-LC3B antibody (red) [Catalog # NB600-1384]. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: LC3B Antibody [NB600-1384] - LC3B staining in treated U87-MG cultured & subcutaneous tumors.

Simple Western: LC3B Antibody [NB600-1384] - Lane view shows a specific band for LC3B in 0.5 mg/ml of Neuro2A lysate at a molecular weight of approximately 15 kDa. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Po, Ba, Bv, Ca, Pm, Ze
• Applications: WB, Simple Western, ELISA, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

LC3B Antibody Summary

• Immunogen: Polyclonal LC3B Antibody was made to a synthetic peptide made to the N-terminal region of the human LC3B protein. [Uniprot: Q9GZQ8]
• Localization: LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
• Marker: Autophagosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP1LC3B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Simple Western 1:100
  • Electron Microscopy
  • ELISA
  • Flow Cytometry 1:200
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 0.1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:400
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Immunoprecipitation
  • Immunohistochemistry Free-Floating
  • Knockdown Validated
  • Knockout Validated
• Application Notes: In Western Blot, bands are seen at ~17 and 19 kDa corresponding to LC3-II and LC3-I. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Electron Microscopy was reported in scientific literature (PMID: 21885071). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 24928515). Use in Immunohistochemistry on both paraffin-embedded and frozen sections reported in scientific literature (PMID: 18259115). Use in immunoprecipitation reported in scientific literature (PMID: 26098573). Use in immunoblotting reported in scientific literature (PMID: 25383539). Use in flow reported in scientific literature (PMID: 27622036). Use in ELISA reported in scientific literature (PMID: 21885071). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 14.688 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Brain Hippocampus Membrane Tissue Lysate (Adult Membrane Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  Neuro2a Whole Cell Lysate
  Neuro2a Chloroquine Treated / Untreated Cell Lysate
  HeLa Chloroquine Treated / Untreated Cell Lysate

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 23724125). Canine and primate reactivity reported in scientific literature (PMID: 24027311) Porcine reactivity reported in scientific literature (PMID: 25378587). Rat reactivity reported in scientific literature (30067379). Bacteria reactivity reported in scientific literature (31110360). Bovine reactivity reported in scientific literature (21868124). Other species have not been tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LC3B Antibody

• Apg8b
• ATG8F
• Autophagy-related protein LC3 B
• Autophagy-related ubiquitin-like modifier LC3 B
• lc3b autophagy marker
• LC3B
• lc3-i, ii
• LC3II
• MAP1 light chain 3-like protein 2
• MAP1A/1BLC3
• MAP1A/MAP1B LC3 B
• MAP1LC3B
• MAP1LC3B-a
• microtubule associated protein 1 light chain 3 beta
• microtubule associated protein 3 b
• microtubule associated protein 3 beta
• microtubule-associated proteins 1A/1B light chain 3
• microtubule-associated proteins 1A/1B light chain 3B

Background

Autophagy (macroautophagy) is a catabolic process which targets intracellular components such as proteins and organelles for degradation. Originally described as a bulk degradation process, current research supports its selective nature (1). Selective autophagy targets specific cellular components for degradation including the endoplasmic reticulum (2) (ER-phagy), mitochondria (3) (mitophagy), peroxisomes (3) (pexophagy), ribosomes (4) (ribophagy) and bacteria (5) (xenophagy). Autophagy relies on a newly formed phagophore, a membrane structure which elongates, sequesters cellular content, and fuses to form a double membrane vesicle known as the autophagosome. Fusion of autophagosomes with lysosomes gives rise to the autophagolysosome, where cellular components are degraded by lysosome hydrolases (1).

Autophagic flux is supported by autophagy-related proteins (Atgs) initially identified in yeast (6,7). The core autophagy machinery is comprised of 17 Atg proteins that play specific roles in autophagosome formation. Among these Atg proteins, Atg8 is not only involved in autophagosome formation but also functions in cargo selection. In mammals, several Atg8 homologues have been identified including microtubule-associated protein 1 light chain 3 alpha, beta and gamma - LC3A, LC3B, and LC3C (8) respectively, as well as GABA type A receptor-associated protein (GABARAP), GABARAP-Like1, and GABARAP-Like2 (9). LC3 (predicted molecular weight 14kD) is ubiquitously expressed and undergoes posttranslational processing after synthesis. First, the cysteine protease Atg4 cleaves a carboxy terminal sequence to generate the cytosolic form LC3-I. Next, E1-like (Atg7) and E2-like (Atg3) enzymes conjugate phosphatidylethanolamine to the newly exposed carboxyterminal glycine, generating LC3-II. Finally, the Atg12-Atg5-Atg16L1 complex participates in LC3 lipidation and autophagosome formation (10). LC3B-I to LC3B-II conversion correlates with autophagosome number and is considered the best marker to monitor autophagy.

References

1. Yu, L., Chen, Y., & Tooze, S. A. (2018). Autophagy pathway: Cellular and molecular mechanisms. Autophagy. https://doi.org/10.1080/15548627.2017.1378838

2. Forrester, A., De Leonibus, C., Grumati, P., Fasana, E., Piemontese, M., Staiano, L.,... Settembre, C. (2019). A selective ER -phagy exerts procollagen quality control via a Calnexin- FAM 134B complex. The EMBO Journal. https://doi.org/10.15252/embj.201899847

3. He, X., Zhu, Y., Zhang, Y., Geng, Y., Gong, J., Geng, J.,... Zhong, H. (2019). RNF34 functions in immunity and selective mitophagy by targeting MAVS for autophagic degradation. The EMBO Journal. https://doi.org/10.15252/embj.2018100978

4. Mathai, B., Meijer, A., & Simonsen, A. (2017). Studying Autophagy in Zebrafish. Cells. https://doi.org/10.3390/cells6030021

5. Losier, T. T., Akuma, M., McKee-Muir, O. C., LeBlond, N. D., Suk, Y., Alsaadi, R. M.,... Russell, R. C. (2019). AMPK Promotes Xenophagy through Priming of Autophagic Kinases upon Detection of Bacterial Outer Membrane Vesicles. Cell Reports. https://doi.org/10.1016/j.celrep.2019.01.062

6. Nakatogawa, H., Suzuki, K., Kamada, Y., & Ohsumi, Y. (2009). Dynamics and diversity in autophagy mechanisms: Lessons from yeast. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm2708

7. Tsukada, M., & Ohsumi, Y. (1993). Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Letters. https://doi.org/10.1016/0014-5793(93)80398-E

8. Wild, P., McEwan, D. G., & Dikic, I. (2014). The LC3 interactome at a glance. Journal of Cell Science. https://doi.org/10.1242/jcs.140426

9. Igloi, G. L. (2001). Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABAA receptor-associated protein. Genomics. https://doi.org/10.1006/geno.2001.6555

10. Glick, D., Barth, S., & Macleod, K. F. (2010). Autophagy: Cellular and molecular mechanisms. Journal of Pathology. https://doi.org/10.1002/path.2697

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LC3B Antibody (NB600-1384)(280)

Publications using NB600-1384

• Tanaka H, Hino H, Moriya S et Al. Comparison of autophagy inducibility in various tyrosine kinase inhibitors and their enhanced cytotoxicity via inhibition of autophagy in cancer cells in combined treatment with azithromycin Biochem Biophys Rep Jul 1 2020 [PMID: 32195376] (IB, Human)
• Kim Hk, Lee Gh, Bhattarai Kr et Al. TMBIM6 (transmembrane BAX inhibitor motif containing 6) enhances autophagy through regulation of lysosomal calcium Autophagy Mar 13 2020 [PMID: 32167007] (Human)
• Wang C, Li Y, Wu X et Al. IRF3 and IRF7 contribute to diesel exhaust particles-induced pulmonary inflammation by mediating mTORC1 activation and restraining autophagy in mice Eur. J. Immunol. Mar 5 2020 [PMID: 32135578] (WB, Mouse)
• Zheng J, Akbari M, Schirmer C et Al. Hippocampal tau oligomerization early in tau pathology coincides with a transient alteration of mitochondrial homeostasis and DNA repair in a mouse model of tauopathy Acta Neuropathol Commun Mar 4 2020 [PMID: 32131898] (WB, Human)
• Vincent G, Novak EA, Siow VS et al. Nix-Mediated Mitophagy Modulates Mitochondrial Damage During Intestinal Inflammation Antioxid. Redox Signal. Mar 31 2020 [PMID: 32103677]
• Bernard M, Yang B, Migneault F et al. Autophagy drives fibroblast senescence through MTORC2 regulation Autophagy Jan 13 2020 [PMID: 31931659] (WB, Human)
• Ando K, De Decker R, Vergara C et al. Picalm reduction exacerbates tau pathology in a murine tauopathy model Acta Neuropathol. Jan 10 2020 [PMID: 31925534] (WB)
• Karuppan MKM, Ojha CR, Rodriguez M, Lapierre J Zika virus infection during pregnancy and induced brain pathology in beclin1-deficient mouse model bioRxiv Nov 15 2019 (WB, Mouse)
• Wardman JH, Henriksen EE, Marthaler AG et al. Enhancement of Autophagy and Solubilization of Ataxin-2 Alleviate Apoptosis in Spinocerebellar Ataxia Type 2 Patient Cells Cerebellum Jan 2 2020 [PMID: 31898278]
• Omary Z Understanding the role of LRBA in patients with inflammatory bowel disease Thesis
• Zhou W, Fang C, Zhang L et al. Thioredoxin domain-containing protein 9 (TXNDC9) contributes to oxaliplatin resistance through regulation of autophagy-apoptosis in colorectal adenocarcinoma Biochemical and Biophysical Research Communications Feb 1 2020 [PMID: 32029274]
• Yoo SY, Yoo JY, Kim HB, et al. Zearalenone Induces Endoplasmic Reticulum Stress and Modulates the Expression of Phase I/II Enzymes in Human Liver Cells Toxins (Basel) Dec 18 2019 [PMID: 31861425] (WB, Human)
• Simon CM, Van Alstyne M, Lotti F et al. Stasimon Contributes to the Loss of Sensory Synapses and Motor Neuron Death in a Mouse Model of Spinal Muscular Atrophy Cell Rep Dec 17 2019 [PMID: 31851921] (WB, Mouse)
• Shao Q, Yang M, Liang C, et al. C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis Autophagy Dec 26 2019 [PMID: 31847700] (ICC/IF, Mouse)
• Zheng Z, Zhou Y, Ye L et al. Histone deacetylase 6 inhibition restores autophagic flux to promote functional recovery after spinal cord injury Exp. Neurol. Nov 30 2019 [PMID: 31794745] (WB, Mouse)
• Yu Y, Xiang N, Lin M et al. miR- 26a Sensitizes Melanoma Cells To Dabrafenib Via Targeting HMGB1-Dependent Autophagy Pathways Drug Des Devel Ther Oct 29 2019 [PMID: 31754297] (WB, Human)
• Codina-MartInez H, FernAndez-GarcIa B, DIez-Planelles C et al. Autophagy is required for performance adaptive response to resistance training and exercise-induced adult neurogenesis Scand J Med Sci Sports Oct 25 2019 [PMID: 31650583]
• Wang R, Wang W, Li A et al. Lipopolysaccharide enhances DNA-induced IFN-beta expression and autophagy by upregulating cGAS expression in A549 cells Exp Ther Med 2019 Nov 01 [PMID: 31602251] (WB, Human)
• Zhang Y, Pan N, Sheng Y et al. Hypoxia enhances IL-10-producing B cell generation through upregulating high-mobility group B1 on tumor cell-released autophagosomes Immunol. Lett. Sep 27 2019 [PMID: 31568811] (Flow, Human)
• Frendo-Cumbo S, Jaldin-Fincati JR, Coyaud E et al. Deficiency of the autophagy gene ATG16L1 induces insulin resistance through KLHL9/KLHL13/CUL3-mediated IRS1 degradation J. Biol. Chem. Sep 12 2019 [PMID: 31515271]
• Hong JM, Kim JH, Kim H et al. SB365, Pulsatilla Saponin D Induces Caspase-Independent Cell Death and Augments the Anticancer Effect of Temozolomide in Glioblastoma Multiforme Cells Molecules Sep 5 2019 [PMID: 31491945] (WB, Human, Mouse)
• Dhandapani PK, Begines-Moreno IM, Brea-Calvo G et al. Hyperoxia but not AOX expression mitigates pathological cardiac remodeling in a mouse model of inflammatory cardiomyopathy Sci Rep Sep 4 2019 [PMID: 31484989] (WB, Mouse)
• Sun CM, Enkhjargal B, Reis C et al. Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats CNS Neurosci Ther Aug 22 2019 [PMID: 31436915] (WB, Rat)
• Schott MB, Weller SG, Schulze RJ et al. Lipid droplet size directs lipolysis and lipophagy catabolism in hepatocytes J. Cell Biol. Aug 7 2019 [PMID: 31391210]
• Parejo S, Tschan MP, Muraro MG et al. Assessing Autophagy During Retinoid Treatment of Breast Cancer Cells Methods Mol. Biol. Jul 31 2019 [PMID: 31359401]
• Geng J, Liu J, Yuan X et al. Andrographolide triggers autophagy-mediated inflammation inhibition and attenuates chronic unpredictable mild stress (CUMS)-induced depressive-like behavior in mice Toxicol. Appl. Pharmacol. Jul 21 2019 [PMID: 31340160] (WB, Mouse)
• Kang S, Son SM, Baik SH, et al. Autophagy-mediated secretory pathway is responsible for both normal and pathological tau in neurons J. Alzheimers Dis. 2019 Jun 24 [PMID: 31256134]
• Cousin MA, Conboy E, Wang JS et al. RINT1 Bi-allelic Variations Cause Infantile-Onset Recurrent Acute Liver Failure and Skeletal Abnormalities Am. J. Hum. Genet. Jul 3 2019 [PMID: 31204009]
• Malvin NP, Kern JT, Liu TC et al. Autophagy proteins are required for club cell structure and function in airways Am. J. Physiol. Lung Cell Mol. Physiol. May 22 2019 [PMID: 31116580] (Mouse)
• Li Z, Zhang S, Li J et al. VacA generates a protective intracellular reservoir for Helicobacter pylori that is eliminated by activation of the lysosomal calcium channel TRPML1 Nat Microbiol May 20 2019 [PMID: 31110360] (ICC/IF, WB, Bacteria)
  
  Details:
  H. pylori
• Ota K, Okuma T, Lorenzo A, Yokota A et al. Fingolimod sensitizes EGFR wild-type non small cell lung cancer cells to lapatinib or sorafenib and induces cell cycle arrest Oncol. Rep. May 2 2019 [PMID: 31059070] (WB)
• Zheng J, Croteau DL, Bohr VA, Akbari M Diminished OPA1 expression and impaired mitochondrial morphology and homeostasis in Aprataxin-deficient cells Nucleic Acids Res. 2019 May 06 [PMID: 30986824] (WB, Human)
• Lee, DH;Ahn, J;Jang, YJ;Ha, TY;Jung, CH; Oleic acid-induced defective autolysosome shows impaired lipid degradation Biochem. Biophys. Res. Commun. Apr 10 2019 [PMID: 30981498] (WB, Human)
• Audesse, AJ;Dhakal, S;Hassell, LA;Gardell, Z;Nemtsova, Y;Webb, AE; FOXO3 directly regulates an autophagy network to functionally regulate proteostasis in adult neural stem cells PLoS Genet. Apr 1 2019 [PMID: 30973875] (WB, Mouse)
• Im EJ, Lee CH, Moon PG et al. Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Nat Commun Mar 27 2019 [PMID: 30918259] (WB, Human)
• van der Kant R, Langness VF, Herrera CM et al. Cholesterol Metabolism Is a Druggable Axis that Independently Regulates Tau and Amyloid-b in iPSC-Derived Alzheimer's Disease Neurons Cell Stem Cell Jan 16 2019 [PMID: 30686764] (WB, Mouse)
• Dubois-Deruy E, Gelinas R, Beauloye C, Esfahani H Beta-3 Adrenoreceptors protect from hypertrophic remodelling through AMP-Activated Protein Kinase and Autophagy. bioRxiv Jan 1 2020 (ICC/IF, Mouse)
• Bhatt V, Khayati K, Hu ZS et al. Autophagy modulates lipid metabolism to maintain metabolic flexibility for Lkb1-deficient Kras-driven lung tumorigenesis Genes Dev. Feb 1 2019 [PMID: 30692209] (WB, Mouse, Human)
• Das LM, Binko AM, Traylor ZP et al. Vitamin D improves sunburns by increasing autophagy in M2 macrophages Autophagy. 2019 May 15 [PMID: 30661440] (Flow, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 form of this antibody.
• Twomey JD, Zhang B. Circulating Tumor Cells Develop Resistance to TRAIL-Induced Apoptosis Through Autophagic Removal of Death Receptor 5: Evidence from an In Vitro Model Cancers (Basel) Jan 15 2019 [PMID: 30650534] (Flow, Human)
• Wang D, Xu Q, Yuan Q et al. Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab. Oncogene Jan 15 2019 [PMID: 30647455] (WB, Human)
• Abdullah M, Greenfield LK, Bronte-Tinkew D et al. VacA promotes CagA accumulation in gastric epithelial cells during Helicobacter pylori infection. Sci Rep Jan 10 2019 [PMID: 30631092] (ICC/IF, WB, Human)
• Ji E, Kim C, Kang H et al. RNA binding protein HuR promotes autophagosome formation by regulating expressions of autophagy-related protein 5, 12, and 16 in human hepatocellular carcinoma cells. Mol. Cell. Biol. Jan 2 2019 [PMID: 30602494] (WB, Human)
• Yu Y, Xie Y, Cao L et al. The Ferroptosis Inducer Erastin Enhances Sensitivity of Acute Myeloid Leukemia Cells to Chemotherapeutic Agents Molecular & Cellular Oncology. 2015 May 26 (WB, Human)
• Chua JPC. Characterizing molecular modifiers of pathogenesis in spinobulbar muscular atrophy. Thesis. 2015 (WB, Rat)
• Saladini S, Aventaggiato M, Barreca F et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells Jan 14 2019 [PMID: 30646605] (WB, Human)
• Chou Li-Fang, Cheng Ya-Lien, Hsieh Chun-Yih et al. Effect of Trehalose Supplementation on Autophagy and Cystogenesis in a Mouse Model of Polycystic Kidney Disease. Nutrients 2018 Nov 6 [PMID: 30585217] (WB, Mouse)
• Spinello I, Saulle E, Quaranta MT et al. The small-molecule compound AC-73 targeting CD147 inhibits leukemic cell proliferation, induces autophagy and increases the chemotherapeutic sensitivity of acute myeloid leukemia cells. Haematologica. Nov 22 2018 [PMID: 30467201] (WB, Human)
• Latre de Late P, Haidar M, Ansari H et al. Theileria highjacks JNK2 into a complex with the macroschizont GPI-anchored surface protein p104 bioRxiv Aug 2 2018 [PMID: 30412643]
• Giuliani A, Cirilli I, Prattichizzo F et al. The mitomiR/Bcl-2 axis affects mitochondrial function and autophagic vacuole formation in senescent endothelial cells. Aging (Albany NY). Oct 21 2018 [PMID: 30348904] (ICC/IF, WB, Human)
• Wang C, Bauckman kA, Ross ASB et al. A non-canonical autophagy-dependent role of the ATG16L1T300A variant in urothelial vesicular trafficking and uropathogenic Escherichia coli persistence. Autophagy. Oct 18 2018 [PMID: 30335568] (WB, Mouse)
• Nawas AF, Mistry R, Narayanan S et al. IL-1 induces p62/SQSTM1 and autophagy in ERa+ /PR+ BCa cell lines concomitant with ERa and PR repression, conferring an ERa- /PR- BCa-like phenotype. J. Cell. Biochem. Oct 15 2018 [PMID: 30324661] (WB, ICC/IF, Human)
• Zhu Y, Fang J, Wang H et al. Baicalin suppresses proliferation, migration, and invasion in human glioblastoma cells via Ca2+-dependent pathway Drug Des Devel Ther Oct 2 2018 [PMID: 30323558] (ICC/IF, WB, Human)
• Janssen AFJ, katrukha EA, van Straaten W et al. Probing aggrephagy using chemically-induced protein aggregates. Nat Commun. Oct 12 2018 [PMID: 30315152] (WB, Human)
• Janser FA, Adams O, Butler V et al. Her2-Targeted Therapy Induces Autophagy in Esophageal Adenocarcinoma Cells. Int J Mol Sci. Oct 8 2018 [PMID: 30297650] (WB, Human)
• Araya J, Tsubouchi k, Sato N et al. PRkN-regulated mitophagy and cellular senescence during COPD pathogenesis. Autophagy. Oct 5 2018 [PMID: 30290714] (Human)
• Li H, Ham A, Ma TC et al. Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations Autophagy Jan 1 2019 [PMID: 30160596] (WB, ICC/IF, Human)
• Dias BRS, de Souza CS, Almeida NJ et al. Autophagic Induction Greatly Enhances Leishmania major Intracellular Survival Compared to Leishmania amazonensis in CBA/j-Infected Macrophages Front Microbiol Aug 15 2018 [PMID: 30158914] (WB, Mouse)
• Tsai YG, Wen YS, Wang JY et al. Complement regulatory protein CD46 induces autophagy against oxidative stress-mediated apoptosis in normal and asthmatic airway epithelium Sci Rep Aug 28 2018 [PMID: 30154478] (WB, Human)
• Langer R, Neppl C, Keller MD et al. Expression Analysis of Autophagy Related Markers LC3B, p62 and HMGB1 Indicate an Autophagy-Independent Negative Prognostic Impact of High p62 Expression in Pulmonary Squamous Cell Carcinomas Cancers (Basel) Aug 21 2018 [PMID: 30134604] (IHC, Human)
• Aubert P, Oleynikova E, Rizvi H et al. Maternal protein restriction induces gastrointestinal dysfunction and enteric nervous system remodeling in rat offspring FASEB J. Aug 1 2018 [PMID: 30067379] (WB, Rat)
• Hutt DM, Mishra Sk, Roth DM et al. Silencing of the Hsp70-specific nucleotide-exchange factor BAG3 corrects the F508del-CFTR variant by restoring autophagy. J. Biol. Chem. Jul 9 2018 [PMID: 29986884] (WB, Human)
• Mauthe M, Orhon I, Rocchi C et al. Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion. Autophagy. Jul 20 2018 [PMID: 29940786] (Human)
• Corona Velazquez A, Corona AK, Klein KA, Jackson WT Poliovirus induces autophagic signaling independent of the ULK1 complex Autophagy. 2018 Sep 19 [PMID: 29929428] (WB, Human)
• Li F, Li Y, Liang H et al. HECTD3 mediates TRAF3 polyubiquitination and type I interferon induction during bacterial infection J. Clin. Invest. Jun 19 2018 [PMID: 29920190] (WB, Mouse)
• Adams O, Janser FA, Dislich B et al. A specific expression profile of LC3B and p62 is associated with nonresponse to neoadjuvant chemotherapy in esophageal adenocarcinomas PLoS ONE Jun 13 2018 [PMID: 29897944] (WB, IHC-P, Human)
• Gao R, Ma J, Wen Z et al. Tumor cell-released autophagosomes (TRAP) enhance apoptosis and immunosuppressive functions of neutrophils Oncoimmunology. 2018 Mar 6 [PMID: 29872581] (WB, Human, Mouse)
  
  Details:
  Citation used the Alexa Fluor 488 format of this antibody.
• Lapierre J, Rodriguez M, Ojha CR, El-Hage N. Critical Role of Beclin1 in HIV Tat and Morphine-Induced Inflammation and Calcium Release in Glial Cells from Autophagy Deficient Mouse J Neuroimmune Pharmacol May 11 2018 [PMID: 29752681] (Mouse)
• Jin J, Britschgi A, SchlAfli AM et al. Low Autophagy (ATG) Gene Expression Is Associated with an Immature AML Blast Cell Phenotype and Can Be Restored during AML Differentiation Therapy Oxid Med Cell Longev. 2018 Mar 17 [PMID: 29743969] (WB, Human)
• Lundquist MR, Goncalves MD, Loughran RM et al. Phosphatidylinositol-5-Phosphate 4-Kinases Regulate Cellular Lipid Metabolism By Facilitating Autophagy Mol. Cell May 3 2018 [PMID: 29727621] (Mouse)
• Shukla Sa, Bachireddy P, Schilling B et al. Cancer-Germline Antigen Expression Discriminates Clinical Outcome to CTLA-4 Blockade. Cell Apr 19 2018 [PMID: 29656892] (Human)
• Saito Y, Moriya S, Kazama H, Hirasawa K. Amino acid starvation culture condition sensitizes EGFR-expressing cancer cell lines to gefitinib-mediated cytotoxicity by inducing atypical necroptosis. Int. J. Oncol. 2018 Feb 23 [PMID: 29484439] (WB, Human)
• Yang JS, Wang CM, Su CH, Ho HC. Eudesmin attenuatesHelicobacter pylori-induced epithelial autophagy and apoptosis and leads to eradication ofH. pyloriinfection. Exp Ther Med. 2018 Mar 01 [PMID: 29456644] (ICC/IF, Human)
• Santiago-O'Farrill JM, Kleinerman ES, Hollomon MG, Livingston A. Phosphorylated heat shock protein 27 as a potential biomarker to predict the role of chemotherapy-induced autophagy in osteosarcoma response to therapy. Oncotarget. 2018 Jan 05 [PMID: 29416717] (WB)
• Bertolo A, Pavlicek D, Gemperli A et al. Increased motility of mesenchymal stem cells is correlated with inhibition of stimulated peripheral blood mononuclear cells in vitro. J Stem Cells Regen Med 2018 Dec 18 [PMID: 29391752]
• Chao TL, Wang TY, Lee CH, Yiin SJ. Anti-Cancerous Effect of Inonotus taiwanensis Polysaccharide Extract on Human Acute Monocytic Leukemia Cells through ROS-Independent Intrinsic Mitochondrial Pathway. Int J Mol Sci. 2018 Jan 29 [PMID: 29382173] (Human)
• Li Y, Guo X, Hu C et al. Type I IFN operates pyroptosis and necroptosis during multidrug-resistant A. baumannii infection Cell Death Differ. 2018 Jul 15 [PMID: 29352265] (WB, Mouse)
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• Sanborn RE, Ross HJ, Aung S et al. A pilot study of an autologous tumor-derived autophagosome vaccine with docetaxel in patients with stage IV non-small cell lung cancer. J Immunother Cancer 2017 Dec 19 [PMID: 29258618] (Flow, Human)
• Magnaghi P, Salom B, Cozzi L et al. Afatinib is a new therapeutic approach in chordoma with a unique ability to target EGFR and Brachyury. Mol. Cancer Ther. 2017 Dec 13 [PMID: 29237806] (Human)
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• Knock E, Matsuzaki S, Takamura H et al. SUMO1 impact on Alzheimer disease pathology in an amyloid-depositing mouse model. Neurobiol. Dis. 2018 Feb 01 [PMID: 29217476] (Mouse)
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• Avinash P, Yann C, Jacques P, Daniela R. Dynamin inhibitors block mTORC1 activation by amino acids independently of dynamin. J Cell Sci. [PMID: 29150487] (WB, Human)
• Zhou H, Luo W, Zeng C et al. PP2A mediates apoptosis or autophagic cell death in multiple myeloma cell lines Oncotarget. 2017 Oct 05 [PMID: 29113343] (WB, Human)
• Liu YH, Wang L, Xu T et al. Whole-genome sequencing of African dogs provides insights into adaptations against tropical parasites. Mol. Biol. Evol. 2017 Oct 10 [PMID: 29040727] (WB, Canine)
• Humbert M, Federzoni EA, Tschan MP. Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL. J. Leukoc. Biol. 2017 Oct 04 [PMID: 28978663] (Human)
• Machiela E, Jeloka R, Caron NS et al. The interaction of aging and oxidative stress contributes to pathogenesis in mouse and human Huntington disease neurons bioRxiv 2019 Oct 10 (ICC/IF, Mouse)
• Parcon PA, Balasubramaniam M, Ayyadevara S et al. Apolipoprotein E4 inhibits autophagy gene products through direct, specific binding to CLEAR motifs Alzheimers Dement 2017 Sep 22 [PMID: 28945989] (Human)
• Kemp V, Dautzenberg IJC, Limpens RW et al. Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery Viruses 2017 Sep 21 [PMID: 28934149] (WB, Human)
• Muniz-Feliciano L, Doggett TA, Zhou Z, Ferguson TA. RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye Autophagy 2017 Sep 21 [PMID: 28933590] (WB)
• Niklaus M, Adams O, Berezowska S et al. Expression analysis of LC3B and p62 indicates intact activated autophagy is associated with an unfavorable prognosis in colon cancer Oncotarget 2017 Aug 15 [PMID: 28903368] (IHC-P, Human)
• Jiang P, Gan M, Yen SH et al. Impaired endo-lysosomal membrane integrity accelerates the seeding progression of a-synuclein aggregates Sci Rep 2017 Aug 09 [PMID: 28794446] (ICC/IF, Human)
• Jia J, Werkmeister E, Gonzalez-Hilarion S et al. Premature termination codon readthrough in human cells occurs in novel cytoplasmic foci and requires UPF proteins J Cell Sci. 2017 Sep 14 [PMID: 28743738] (ICC/IF, Human)
• Wiedmer T, Blank A, Pantasis S et al. Autophagy inhibition improves sunitinib efficacy in pancreatic neuroendocrine tumors via a lysosome-dependent mechanism Mol. Cancer Ther. 2017 Jul 20 [PMID: 28729403] (Human)
• Germic N, Stojkov D, Oberson K, et al. Neither eosinophils nor neutrophils require ATG5-dependent autophagy for extracellular DNA trap formation Immunology 2017 Jul 13 [PMID: 28703297] (Mouse)
• Cao B, Parnell LA, Diamond MS, Mysorekar IU. Inhibition of autophagy limits vertical transmission of Zika virus in pregnant mice J. Exp. Med. 2017 Jul 10 [PMID: 28694387] (WB, Mouse)
• Sun QZ, Lin GF, Li LL et al. Discovery of Potent and Selective Inhibitors of Cdc2-Like Kinase 1 (CLK1) as a New Class of Autophagy Inducers J. Med. Chem. 2017 Jul 27 [PMID: 28692292] (WB)
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• Tuohetahuntila M, Molenaar MR, Spee B et al. Lysosome-mediated degradation of a distinct pool of lipid droplets during hepatic stellate cell activation journal of biological chemistry 2017 Jun 14 [PMID: 28615446] (WB, Rat)
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• Saha SK, Yin Y, Kim K et al. Valproic Acid Induces Endocytosis-Mediated Doxorubicin Internalization and Shows Synergistic Cytotoxic Effects in Hepatocellular Carcinoma Cells. Int J Mol Sci May 12 2017 [PMID: 28498322] (WB, Human)
• Pagliassotti MJ, Estrada AL, Hudson WM et al. Trehalose supplementation reduces hepatic endoplasmic reticulum stress and inflammatory signaling in old mice. J. Nutr. Biochem. Apr 6 2017 [PMID: 28431320] (WB, Mouse)
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• Hung HH, Kao LS, Liu PS et al. Dopamine elevates intracellular zinc concentration in cultured rat embryonic cortical neurons through the cAMP-nitric oxide signaling cascade. Mol. Cell. Neurosci. Apr 17 2017 [PMID: 28427888] (WB, Rat)
• Zhu Y, Shui M, Liu X et al. Increased autophagic degradation contributes to the neuroprotection of hydrogen sulfide against cerebral ischemia/reperfusion injury. Metab Brain Dis. Apr 18 2017 [PMID: 28421304] (WB, Mouse)
• Sioutas A, Vainikka LK, Kentson M et al. Oxidant-induced autophagy and ferritin degradation contribute to epithelial-mesenchymal transition through lysosomal iron. J Inflamm Res. Apr 13 2017 [PMID: 28405169] (WB, Human)
• Tang L, Chai W, Ye F et al. HMGB1 promotes differentiation syndrome by inducing hyperinflammation via MEK/ERK signaling in acute promyelocytic leukemia cells. Oncotarget Apr 18 2017 [PMID: 28404891] (WB, Human)
• Gao Y, Zhuang Z, Gao S et al. Tetrahydrocurcumin reduces oxidative stress-induced apoptosis via the mitochondrial apoptotic pathway by modulating autophagy in rats after traumatic brain injury. Am J Transl Res 2017 Mar 15 [PMID: 28386319] (WB, Rat)
• Liu X, Cao H, Li J et al. Autophagy induced by DAMPs facilitates the inflammation response in lungs undergoing ischemia-reperfusion injury through promoting TRAF6 ubiquitination. Cell Death Differ. Apr 1 2017 [PMID: 28157209] (WB, ICC/IF, Porcine)
• Tomoda T, Sumitomo A, Hirota-Tsuyada Y et al. BDNF controls neuropsychiatric manifestation via autophagic regulation of p62 and GABAA receptor trafficking bioRxiv May 30 2018 (WB, Mouse)
• Lu Y, Bradley JS, McCoski SR et al. Reduced skeletal muscle fiber size following caloric restriction is associated with calpain-mediated proteolysis and attenuation of IGF-1 signaling. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2017 Feb 22 [PMID: 28228415]
• Wang J, Cao B, Han D et al. Long Non-coding RNA H19 Induces Cerebral Ischemia Reperfusion Injury via Activation of Autophagy. Aging Dis 2017 Feb [PMID: 28203482]
• Liu YH, Weng YP, Lin HY et al. Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells. Sci Rep. 2017 Jan 30 [PMID: 28134285] (WB, Human)
  
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• Kristensen LV, Oppermann FS, Rauen MJ et al. Polyglutamine expansion of ataxin-3 alters its degree of ubiquitination and phosphorylation at specific sites Neurochem. Int Jan 6 2017 [PMID: 28065793]
• Li Z, Schulze RJ, Weller SG et al. A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets Sci Adv. 2016 Dec 15 [PMID: 28028537] (WB, Human)
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• Qi X, Man SM, Malireddi RK et al. Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection. J. Exp. Med. Aug 22 2016 [PMID: 27551156] (WB, ICC/IF, Mouse)
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• Cemma M, Grinstein S, Brumell JH. Autophagy Proteins are not Universally Required for Phagosome Maturation. Autophagy Jun 16 2016 [PMID: 27310610] (WB)
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• Lee SH, Du J, Stitham J et al. Inducing mitophagy in diabetic platelets protects against severe oxidative stress. EMBO Mol Med. 2016 May 23 [PMID: 27221050] (FLOW, Human)
  
  Details:
  This publication used the AF488 conjugated form of this antibody (NB600-1384AF488)
• Alan L, Spacek T, Pajuelo Reguera D et al. Mitochondrial nucleoid clusters protect newly synthesized mtDNA during Doxorubicin- and Ethidium Bromide-induced mitochondrial stress. Toxicol. Appl. Pharmacol. 2016 Apr 19 [PMID: 27102948] (WB, Human)
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  Details:
  LC3B/MAP1LC3B antibody was used for WB and ICC-IF assays on samples from HeLa cells which were stably knocked down for LAPTM4b or control and were starved - treated with 50?uM chloroquine. ICC-IF assay employed 4% paraformaldehyde fixation, permeablization for 15?minutes with cold 95% methanol, 1 hour blocking with 1% BSA, use of primary at 1:200 dilution with 4C ON incubation.
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  Details:
  LC3B antibody used for WB at dilution 1:2500 on liver lysates of naive, Nlrp3-/-, Casp1-/-, Ifnar1-/- and WT mice (Figure 10A).
• Lee Jm, Wagner M, Xiao R et al. Nutrient-sensing nuclear receptors coordinate autophagy. Nature. 2014 Nov 12 [PMID: 25383539]
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  Details:
  LC3B/MAP1LC3B antibody used for WB on porcine primary retinal pigment epithelium/RPE cells.
• Wang J, Gu S, Huang J et al. Inhibition of autophagy potentiates the efficacy of Gli inhibitor GAnT-61 in MYCn-amplified neuroblastoma cells. BMC Cancer. 2014 Oct 23 [PMID: 25323222] (WB, Human)
• Mathew TS, Ferris RK, Downs RM et al. Caffeine promotes autophagy in skeletal muscle cells by increasing the calcium-dependent activation of AMP-activated protein kinase. Biochem. Biophys. Res. Commun. 2014 Sep 28 [PMID: 25268764] (WB, ICC/IF, Mouse)
  
  Details:
  LC3B antibody used for WB and ICC-IF in experiments involving C2C12 cells (mouse myoblast cell line).
• Juarez E, Carranza C, Hernandez-Sanchez F et al. Nucleotide-oligomerizing domain-1 (NOD1) receptor activation induces pro-inflammatory responses and autophagy in human alveolar macrophages. BMC Pulm Med. 2014 Sep 25 [PMID: 25253572] (WB, EM, Human)
• Pacheco FJ, Almaguel FG, Evans W et al. Docosahexanoic acid antagonizes TNF-alpha-induced necroptosis by attenuating oxidative stress, ceramide production, lysosomal dysfunction, and autophagic features. Inflamm Res 2014 Oct [PMID: 25095742] (WB)
• Vodicka P, Lim J, Williams DT et al. Assessment of Chloroquine Treatment for Modulating Autophagy Flux in Brain of WT and HD Mice. J Huntingtons Dis 2014 Jul 02 [PMID: 25062859] (WB, Mouse)
  
  Details:
  LC3B antibody used for WB in lysates of brain and muscle tissues of WT and HD (Q175/Q175) mice that received or not the intraperitoneal injections of chloroquine.
• Haimovici Aladin, Brigger Daniel, Torbett Bruce E et al. Induction of the autophagy-associated gene MAP1S via PU.1 supports APL differentiation. Leuk Res. 2014 Jul 01 [PMID: 25043887] (Human)
• Li Y, Chen C, Yao F et al. AMPK inhibits cardiac hypertrophy by promoting autophagy via mTORC1. Arch. Biochem. Biophys. 2014 Jul 04 [PMID: 25009141] (WB, Rat, Mouse)
  
  Details:
  LC3B antibody used for WB on hearts of male C57BL/6J mice subjected or not (sham) to cardiac hypertrophy induced by pressure overload performed by descending aortic banding/AB (Fig. 1A). Antibody also used for WB on sham or AB groups treated or not with AMPK activator AICAR or metformin. LC3B WB also performed on cultured Rat's cardiomyocytes transfected with Ad-CA-AMPK or Ad-GFP - treated or not with phenylephrine/PE and/or Rapamycin (Fig. 4A, 4C; Fig. 6C).
• Brigger D, Proikas-Cezanne T, Tschan MP. WIPI-dependent autophagy during neutrophil differentiation of NB4 acute promyelocytic leukemia cells. Cell Death Dis 2014 Jul 04 [PMID: 24991767] (WB, ICC/IF, Human)
  
  Details:
  LC3B antibody used for WB for measuring autophagic flux in SHC002 and shWIPI-1 or shWIPI-2 expressing NB4 cells (human acute promyelocytic leukemic/APL cell lines NB4) upon 1 uM ATRA treatment at day 4 in the presence or absence of bafilomycin A1 (Figure 4a). Antibody also used for ICC-IF application.
• Zhou Y,Sun K,Ma Y et al. Autophagy inhibits chemotherapy-induced apoptosis through downregulating Bad and Bim in hepatocellular carcinoma cells Sci Rep. 2014 Jun 20 [PMID: 24947039] (WB, Human)
  
  Details:
  LC3B antibody used for WB in human hepatocellular carcinoma cells/HCC cell line SMMC-7721 and HepG2 (transiently transfected with GFP-LC3 plasmids) cultured under low glucose and hypoxia (LH) conditions in the presence or absence of CQ or 3-MA for 8 h (Figure 1C).
• Sheng R, Zhang TT, Felice VD et al. Preconditioning stimuli induce autophagy via sphingosine kinase 2 in mouse cortical neurons. J. Biol. Chem. 2014 Jun 13 [PMID: 24928515] (ICC/IF, IHC-FrFl, Mouse)
  
  Details:
  Used for ICC-IF on murine cortical neurons and IHC-FrFl on mouse brain free floating sections. ICC-IF - Neurons grown on coverglasses fixed for 15 min with 4% PFA and permeablized with PBS/0.1% Triton X-100 for 30 min, blocked with 2% BSA for 1 h at RT, incubated with LC3 (1:800; Novus; NB600-1384) or SPK2 (1:500) at 4°C for 24 h followed by Cy3-conjugated anti-rabbit IgG for 2 h (FIGURE 2A). IHC-FrFl - 24h past isoflurane, C57 mice perfused with PBS/4% PFA-PBS, brains postfixed in 4% PFA/ON, 40 micron-thick coronal sections cut and blocked in 5% normal goat serum, 1% BSA, 0.2% Triton X-100, 0.03% H2O2 in PBS/1 h RT, incubated with 1:800 dilution of LC3-antibody for 48 h at 4C followed by biotinylated anti-rabbit secondary antibody/2 h RT and ABC -DAB detection (FIGURE 6C).
• Yang Annan, Rajeshkumar N V, Wang Xiaoxu et al. Autophagy is critical for pancreatic tumor growth and progression in tumors with p53 alterations. Cancer Discov. 2014 May 29 [PMID: 24875860]
• Karsli-Uzunbas G, Guo JY, Price SM et al. Autophagy is Required for Glucose Homeostasis and Lung Tumor Maintenance. Cancer Discov. 2014 May 29 [PMID: 24875857] (WB, Mouse)
• Stein LR, Imai SI. Specific ablation of Nampt in adult neural stem cells recapitulates their functional defects during aging. EMBO J. 2014 May 08 [PMID: 24811750] (WB, Mouse)
• Stein LR, Wozniak DF, Dearborn JT et al. Expression of nampt in hippocampal and cortical excitatory neurons is critical for cognitive function. J. Neurosci. 2014 Apr 18 [PMID: 24760840] (WB, Mouse)
• Pang Y, Si M, Sun B et al. DHA2, a synthesized derivative of bisbibenzyl, exerts antitumor activity against ovarian cancer through inhibition of XIAP and Akt/mTOR pathway. Food Chem. Toxicol. 2014 Apr 23 [PMID: 24751974] (WB, ICC/IF, Human)
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• Akbari Mansour, Keijzers Guido, Maynard Scott et al. Overexpression of DNA ligase III in mitochondria protects cells against oxidative stress and improves mitochondrial DNA base excision repair. DNA Repair (Amst). 2014 Feb 27 [PMID: 24674627]
• Stellrecht CM, Vangapandu HV, Le XF et al. ATP directed agent, 8-chloro-adenosine, induces AMP activated protein kinase activity, leading to autophagic cell death in breast cancer cells. J Hematol Oncol 3/21/2014 [PMID: 24628795] (WB, Human)
• Cebotaru V, Cebotaru L, Kim H et al. Polycystin-1 negatively regulates polycystin-2 expression via the aggresome/autophagosome pathway. J. Biol. Chem. 2014 Jan 24 [PMID: 24459142] (WB, Canine)
• Takasaka N, Araya J, Hara H et al. Autophagy Induction by SIRT6 through Attenuation of Insulin-like Growth Factor Signaling Is Involved in the Regulation of Human Bronchial Epithelial Cell Senescence. J Immunol 2013 Dec 23 [PMID: 24367027] (WB, Human)
• Lai AY, Lan CP, Hasan S et al. scyllo-Inositol promotes robust mutant Huntingtin protein degradation. J Biol Chem 2013 Dec 18 [PMID: 24352657] (WB, Rat)
• Huang YL, Chang CL, Tang CH et al. Extrinsic sphingosine 1-phosphate activates S1P5 and induces autophagy through generating endoplasmic reticulum stress in human prostate cancer PC-3 cells. Cell Signal 2013 Dec 11 [PMID: 24333325] (WB, Human)
• Han J, Jung S, Jang J et al. OCIAD2 activates gamma-secretase to enhance amyloid beta production by interacting with nicastrin. Cell Mol Life Sci. 2013 Nov 24 [PMID: 24270855] (WB, Human)
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• Chua JP, Reddy SL, Merry DE et al. Transcriptional activation of TFEB/ZKSCAN3 target genes underlies enhanced autophagy in spinobulbar muscular atrophy. Hum Mol Genet. 2013 Oct 31 [PMID: 24150846] (WB, Mouse)
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• Ott CJ, Kopp N, Bird L et al. BET bromodomain inhibition targets both c-Myc and IL7R in high-risk acute lymphoblastic leukemia Blood 2012 Oct 4 [PMID: 22904298]
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• Scheibye-Knudsen M, Ramamoorthy M, Sykora P, Maynard S, Lin PC, Minor RK, Wilson Iii DM, Cooper M, Spencer R, de Cabo R, Croteau DL, Bohr VA. Cockayne syndrome group B protein prevents the accumulation of damaged mitochondria by promoting mitochondrial autophagy. J Exp Med;209(4):855-69. 2012 Apr 9. [PMID: 22473955] (WB, Human)
• Delk NA, Farach-Carson MC. Interleukin-6: a bone marrow stromal cell paracrine signal that induces neuroendocrine differentiation and modulates autophagy in bone metastatic PCa cells Autophagy 2012 Apr [PMID: 22441019]
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• Kawano A, Tsukimoto M, Mori D et al. Regulation of P2X7-dependent inflammatory functions by P2X4 receptor in mouse macrophages Biochem Biophys Res Commun. 2012 Mar 30 [PMID: 22405772] (WB, Mouse)
• Dehvari N, Mahmud T, Persson J et al. Amyloid precursor protein accumulates in aggresomes in response to proteasome inhibitor. Neurochem Int 2012 Apr [PMID: 22366649]
  
  Details:
  Using the Biotin conjugated version of NB600-1384, catalog number NB600-1384B.
• Congdon EE, Wu JW, Myeku N et al. Methylthioninium chloride (methylene blue) induces autophagy and attenuates tauopathy in vitro and in vivo Autophagy 2012 Apr [PMID: 22361619]
• Ma X, Jin M, Cai Y et al. Mitochondrial electron transport chain complex III is required for antimycin A to inhibit autophagy. Chem Biol 2011 Nov 23 [PMID: 22118681]
• Zheng L, Terman A, Hallbeck M et al. Macroautophagy-generated increase of lysosomal amyloid b-protein mediates oxidant-induced apoptosis of cultured neuroblastoma cells. Autophagy 2011 [PMID: 22108004]
  
  Details:
  Using the Biotin conjugated version of NB600-1384, catalog number NB600-1384B.
• AnPK, Tait SW, Lamkanfi M et al. TLR2 and RIP2 pathways mediate autophagy of Listeria monocytogenes via ERK activation. J Biol Chem. 2011 Oct 27. [PMID: 22033934]
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• Sobolewska A, Motyl T, Gajewska M. Role and regulation of autophagy in the development of acinar structures formed by bovine BME-UV1 mammary epithelial cells. Eur J Cell Biol. 2011 Aug 23. [PMID: 21868124] (WB, Bovine)
• Castino R, Fiorentino I, Cagnin M et al. Chelation of lysosomal iron protects dopaminergic SH-SY5Y neuroblastoma cells from hydrogen peroxide toxicity by precluding autophagy and Akt dephosphorylation. Toxicol Sci. 2011 Jul 8. [PMID: 21742779]
• Desmolaize B, Rose S, Wilhelm C et al. Combinations of macrolide resistance determinants in field isolates of Mannheimia haemolytica and Pasteurella multocida. Antimicrob Agents Chemother 2011 Sep [PMID: 21709086]
• Ling Y-H, Aracil M, Zou Y et al. PM02734 Induces Caspase-Independent Cell Death Associated with Features of Autophagy, Inhibition of the Akt/mTOR Signaling Pathway, and Activation of DAP Kinase. Clin Cancer Res. 2011 Jun 20. [PMID: 21690574] (WB, IHC-P, Human)
• Crisan TO, Plantinga TS, van de Veerdonk FL et al. Inflammasome-Independent Modulation of Cytokine Response by Autophagy in Human Cells. PLoS ONE. 2011 Apr 7. [PMID: 21490934]
• Haspel J, Shaik RS, Ifedigbo E et al. Characterization of macroautophagic flux in vivo using a leupeptin-based assay. Autophagy 2011 Jun [PMID: 21460622] (WB, Mouse)
• Yang S, Wang X, Contino G et al. Pancreatic cancers require autophagy for tumor growth. Genes Dev;25(7):717-29. 2011 Apr 1. [PMID: 21406549]
• Xu P, Das M, Reilly J, Davis RJ. JNK regulates FoxO-dependent autophagy in neurons. Genes Dev;25(4):310-322. 2011 Feb 15. [PMID: 21325132] (WB, IHC-P, ICC/IF, Mouse)
• Kudo LC, Parfenova L, Ren G et al. Puromycin-Sensitive Aminopeptidase (PSA/NPEPPS) Impedes Development of Neuropathology in hPSA/TAUP301L Double Transgenic Mice. Hum Mol Genet. 2011 Feb 14. [PMID: 21320871]
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Reviews for LC3B Antibody (NB600-1384) (18)

Average Rating: 4.2

(Based on 18 reviews)

We have 18 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Anonymous WB Mouse 07/17/2019

Summary

• Application: Western Blot
• Sample Tested: Bone Marrrow Derived Macrophages
• Species: Mouse
• Lot: CF-1

Comments

• Comments: Secondary antibody used: Alexa Fluor 488

reviewed by: Anonymous WB Human 06/04/2018

Summary

• Application: Western Blot
• Sample Tested: Hek 293T cell lysate
• Species: Human
• Lot: CC-1

reviewed by: Bo Ding WB Mouse 02/20/2018

Summary

• Application: Western Blot
• Sample Tested: 20 ug whole cell lysate
• Species: Mouse
• Lot: CJ-1

reviewed by: Anonymous WB Mouse 07/22/2016

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Mouse
• Lot: BQ

Blocking

• Blocking Details: 5% skim milk in TBS

Primary Anitbody

• Dilution Ratio: 1:1,000

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG, HRP
• Secondary Concentration: 0.4 ug/ml

Details

• Detection Notes: ECL, exposure 30 seconds

reviewed by: Anonymous WB Mouse 06/28/2016

Summary

• Application: Western Blot
• Sample Tested: mice primary macrophages
• Species: Mouse
• Lot: BO

Blocking

• Blocking Details: 5% milk for 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: 4 degree overnight, 1:1,000 dilution

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG, HRP
• Secondary Concentration: 0.4 ug/ml

Details

• Detection Notes: with ECL solution, 25 secs of exposure time.

reviewed by: Anonymous Human 02/04/2015

Summary

• Species: Human
• File: View PDF

reviewed by: Anonymous IF Mouse 12/23/2014

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23525089
• Species: Mouse

Blocking

• Blocking Details: See PMID 23525089

Details

• Detection Notes: See PMID 23525089
• Fixation Details: Methanol fixation required.

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 23525089.

reviewed by: Anonymous IF Rat 12/23/2014

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23688351
• Species: Rat

Blocking

• Blocking Details: See PMID 23688351

Details

• Detection Notes: See PMID 23688351

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 23688351.

reviewed by: Anonymous WB Human 11/25/2014

Summary

• Application: Western Blot
• Sample Tested: IB3-1 whole cell lysate
• Species: Human
• Lot: trial sample

Blocking

• Blocking Details: 3% non-fat dry milk (Bio-Rad) in TBST, 30 min at room temperature

Primary Anitbody

• Dilution Ratio: 1:1000 dilution in blocking buffer, overnight at 4 degree

Secondary Antibody

• Secondary Description: anti-rabbit IgG, HRP
• Secondary Manufacturer Cat#: Cell signaling, 7074S
• Secondary Concentration: 1:5000 dilution

Details

• Detection Notes: film detection, using Clarity Western ECL (Bio-Rad) for 3 min.

reviewed by: Anonymous WB Mouse 11/23/2014

Summary

• Application: Western Blot
• Sample Tested: mouse hearts
• Species: Mouse

Blocking

• Blocking Details: BSA

Primary Anitbody

• Dilution Ratio: 1:2000

Secondary Antibody

• Secondary Description: rabbit anti-mouse IgG, HRP
• Secondary Concentration: 1:10000

Details

• Detection Notes: exposure time 1 min

reviewed by: Anonymous WB Rat 11/01/2014

Summary

• Application: Western Blot
• Sample Tested: Rat brain
• Species: Rat
• Comments: Works best with milk, detect 2 nice bands.

Blocking

• Blocking Details: Milk 10%in PBST for 2 hrs at RT

Primary Anitbody

• Dilution Ratio: 1/2000 for 6 hrs at 4 degrees

Secondary Antibody

• Secondary Description: Donkey anti Rabbit HRP
• Secondary Manufacturer Cat#: Pierce
• Secondary Concentration: 1/10000

Details

• Detection Notes: ECL with biorad chemidoc XRS

Comments

• Comments: Works best with milk, detect 2 nice bands.

reviewed by: Anonymous WB Mouse 10/03/2014

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate alveolar macrophages
• Species: Mouse

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG HRP

reviewed by: Mohammad Abedi WB Human 07/10/2014

Summary

• Application: Western Blot
• Sample Tested: Neuroblastoma whole cell lysate
• Species: Human
• Lot: 1384

Blocking

• Blocking Details: 5% milk solution overnight at 4oC

Primary Anitbody

• Dilution Ratio: 1:1000, 1 hour Incubation at RT in TBST

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG- HRP Conjugated
• Secondary Manufacturer Cat#: #7074S
• Secondary Concentration: 1:1000 in TBST for 1 hour at RT

Details

• Detection Notes: Densitometry, VersaDoc MP 5000, 300 seconds

reviewed by: Anony- mous WB Human 06/10/2014

Summary

• Application: Western Blot
• Species: Human
• Special Applications: Western blot analysis of extracts from HeLa and HT1080 cells using LC3B antibody (NB600-1384, 1:1000).
• File: View PDF

reviewed by: Anony- mous ICC Human 06/10/2014

Summary

• Application: Immunocytochemistry
• Species: Human
• Special Applications: IF Confocal analysis of HeLa cells using LC3B antibody (NB600-1384, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
• File: View PDF

reviewed by: Anonymous IHC Human 10/19/2010

Summary

• Application: Immunohistochemistry
• Sample Tested: Human Esophagus tissue
• Species: Human

Details

• Fixation Details: Sample Preparation: FFPE

reviewed by: Anonymous IF Other 08/28/2009

Summary

• Application: Immunofluorescence
• Sample Tested: Human Oral Keratinocyte
• Species: Other
• Lot: K
• Comments: It may be that the LC3 protein is difficult to detect in autophagosomes in Keratinocytes.
  
  Not yet tested in HeLa.

Blocking

• Blocking Details: Blocking Buffer: BSA, Blocking Time: 1 hour, Blocking Temp: 22 degrees Celsius

Primary Anitbody

• Dilution Ratio: Primary Ab Incubation Dilution Buffer: 2% BSA, Primary Ab Dilution Ratio: 1/2000, Incubation Time: 16hr, Incubation Temp: 4C

Secondary Antibody

• Secondary Description: Secondary Ab: Molecular Probes, Secondary Ab Dilution: 1/200
• Secondary Manufacturer Cat#: A-11034

Comments

• Comments: It may be that the LC3 protein is difficult to detect in autophagosomes in Keratinocytes.
  
  Not yet tested in HeLa.

reviewed by: Anonymous WB Mouse 05/26/2009

Summary

• Application: Western Blot
• Sample Tested: mouse liver tissue, Sample Amount: 100
• Species: Mouse
• Lot: I1

Blocking

• Blocking Details: Blocking Buffer: 5% milk/PBS, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1/2000, Incubation Dilution Buffer: milk/PBS, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

• Secondary Description: Secondary Ab: goat-anti rabbit-HRP (Pierce), Secondary Ab Dilution Ratio: 1/2000

Details

• Detection Notes: Detection Method: ECL-x-ray, Exposure Time: 1 minute, Specific Bands: 15 kDa, Non-Specific Bands: 20 kDa, 37 kDa, 82 kDa

FAQs for LC3B Antibody (NB600-1384). (Showing 1 - 5 of 5 FAQs).

1. Hello, somewhere on the LC3B antibody page I read it is recommended to use a 0.2uM membrane for western blot?
   • Yes, because LC3B-I is about 14-15 kDa and LC3B-II is even even smaller, the protein may slip through the 0.45 uM membrane and be lost during blotting. However, there has been success using 0.45 uM membranes; a 0.2 uM membrane is something to consider if no signal is detected.
2. What is the immunogen sequence of this antibody?
   • The exact immunogen sequence is proprietary, however we can tell you that the immunogen sequence can be found between amino acids 1-50 of human LC3B.
3. Does this antibody also recognize mouse LC3A?
   • This antibody does not cross-react with LC3A. If you are interested in detecting mouse LC3A and LC3B, you may want to consider purchasing our LC3/LC3B antibody pack, NB910-40752.
4. Is it possible to provide me the suggested dilution of Electron Microscopy and IHC of NB600-1384?
   • Electron microscopy was performed by a customer, so unfortunately, we do not have their dilution information. For IHC, we recommend a dilution of 1:400.
5. What % gel used for WB to obtain the separation of the LC3BI and II bands?
   • We prefer to use a 4-20% gradient gel to get ample separation between the LC3BI and II bands. If you are using a single percentage gel, a 12% or higher gel should separate the lower weights enough for proper detection.

Bioinformatics

• Gene Symbol: MAP1LC3B