Human Brain Whole Tissue Lysate (Adult Whole Normal)

Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA
• Concentration: 5.0 mg/ml

Human Brain Whole Tissue Lysate (Adult Whole Normal) Summary

• Specificity: Total Protein - Human Adult Normal Tissue: Brain

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 10 ug - 20 ug
• Application Notes: Use in ELISA reported in scientific literature (PMID 27120609).

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Brain
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

This lysate was produced from total protein - Human Adult Normal Tissue: Brain. Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Brain Lysate (NB820-59177)(19)

Publications using NB820-59177

• Capano L, Sato C, Ficulle E et al. Recapitulation of endogenous 4R tau expression and formation of insoluble tau in directly reprogrammed human neurons Cell Stem Cell 2022-06-01 [PMID: 35659876] (NAE, Human)
• Tapanes SA, Arizanovska D, Diaz MM et al. Inhibition of glial D-serine release rescues synaptic damage after brain injury Glia 2022-02-23 [PMID: 35195906]
• Puvvula PK, Yu Y, Sullivan KR et al. Inhibiting an RBM39/MLL1 epigenomic regulatory complex with dominant-negative peptides disrupts cancer cell transcription and proliferation Cell reports 2021-06-01 [PMID: 34077726]
• Tacke S, Braune S, Rovituso DM et al. B-Cell Activity Predicts Response to Glatiramer Acetate and Interferon in Relapsing-Remitting Multiple Sclerosis Neurology(R) neuroimmunology & neuroinflammation 2021-05-01 [PMID: 33707177] (ESPOT)
• Guihurt Santiago J, Burgos-Tirado N, Lafontaine DD, et al. Adhesion G protein-coupled receptor, ELTD1, is a potential therapeutic target for retinoblastoma migration and invasion BMC cancer 2021-01-11 [PMID: 33430814] (WB)
• Chen HY, Lin CH, Teng SC Stress-induced p53 drives BAG5 cochaperone expression to control alpha-synuclein aggregation in Parkinson's disease Aging (Albany NY) 2020-10-21 [PMID: 33085644] (IP)
• Kuerten S, Lanz TV, Lingampalli N et al. Autoantibodies against central nervous system antigens in a subset of B cell-dominant multiple sclerosis patients Proc. Natl. Acad. Sci. U.S.A. 2020-09-01 [PMID: 32817492]
• Parkhouse RME, Carpio A, Cortez MM et al. Anti-brain protein autoantibodies are detectable in extraparenchymal but not parenchymal neurocysticercosis J. Neuroimmunol. 2020-04-06 [PMID: 32305783] (MS, Human)
• Liao WC, Liao CK, Tseng TJ et al. Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability Oncogenesis 2020-02-04 [PMID: 32019907] (WB, Human)
• Hwang M, Han MH, Park HH et al. LGR5 and Downstream Intracellular Signaling Proteins Play Critical Roles in the Cell Proliferation of Neuroblastoma, Meningioma and Pituitary Adenoma Exp Neurobiol 2019-10-31 [PMID: 31698554]
• Ventero MP, Fuentes-Baile M, Quereda C et al. Radiotherapy resistance acquisition in Glioblastoma. Role of SOCS1 and SOCS3 PLoS ONE 2019-02-27 [PMID: 30811476] (WB, Human)
• Majumder P, Roy K, Singh BK et al. Cellular levels of growth factor receptor bound protein 2 (Grb2) and cytoskeleton stability are correlated in a neurodegenerative scenario. Dis Model Mech. 2017-03-30 [PMID: 28360125] (WB)
• Saito K, Ohta S, Kawakami Y et al. Functional analysis of KIF20A, a potential immunotherapeutic target for glioma. J Neurooncol 2017-03-01 [PMID: 28070829]
• Wunsch M, Hohmann C, Milles B et al. The Correlation between the Virus- and Brain Antigen-Specific B Cell Response in the Blood of Patients with Multiple Sclerosis. Viruses. 2016-04-28 [PMID: 27120609] (ELISA)
• Rasmussen RD, Gajjar MK, Jensen KE et al. Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma. Mol Oncol. 2016-01-08 [PMID: 26794465] (WB)
• Choi I, Kim B, Byun JW et al. LRRK2 G2019S mutation attenuates microglial motility by inhibiting focal adhesion kinase. Nat Commun 2015-09-14 [PMID: 26365310] (IP)
• Categorization of multiple sclerosis relapse subtypes by B cell profiling in the blood. Hohmann C, Milles B, Schinke M Acta Neuropathol Commun [PMID: 25597707] (ELISA, Human)
• Molina J, Rodriguez-Diaz R, Fachado A et al. Control of Insulin Secretion by Cholinergic Signaling in the Human Pancreatic Islet. Diabetes 2014-08-01 [PMID: 24658304] (WB, Human)
• Kuerten S, Pommerschein G, Barth SK et al. Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood. Clin. Immunol. 2014-03-05 [PMID: 24607792] (ELISPOT, Human)

Review for Brain Lysate (NB820-59177) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/03/2014

Summary

• Application: Western Blot
• Sample Tested: Human Brain Whole Tissue Lysate (Adult Normal), Novus NB820-59177
• Species: Human
• Lot: A906100

FAQs for Brain Lysate (NB820-59177). (Showing 1 - 5 of 5 FAQs).

1. We are seeking a normal brain tissue lysate for control of Parkinson’s disease. In your website, there is Human Brain Parietal Lobe Whole Tissue Lysate (Adult Parkinson's) 1.0mg (NB820-59414). If you have any normal brain tissue lysate, please let me know.
   • Please see this link to all of our normal human brain tissue lysates.
2. I have a question regarding your product no. NB820-59177. I would like to use this lysate in an ELISPOT assay and I am not sure if this is possible. My particular question is if this product is sterile, i.e. can it be used for cell culture?
   • Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.
3. May we ask how much we shall load by using NB820-59177 as positive control in WB?
   • We recommend loading 10-20ug of lysate per well for Western blot.
4. Is there a recommended expiration date for the Human Brain Whole Tissue Lysate, cat:NB820-59177, Lot: B703069?
   • You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.
5. May we ask if the Human Brain Whole Tissue Lysate (cat. no. NB820-59177) is suitable for RNA extraction?
   • Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.