Human Brain Whole Tissue Lysate (Adult Whole Normal)

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Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Concentration
5.0 mg/ml

Order Details

Human Brain Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Brain

Applications/Dilutions

Dilutions
  • Western Blot 10 ug - 20 ug
Application Notes
Use in ELISA reported in scientific literature (PMID 27120609).
Reviewed Applications
Read 1 Review rated 5
using
NB820-59177 in the following application:

Publications
Read Publications using
NB820-59177 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Concentration
5.0 mg/ml

Lysate Details for Brain

Type
Tissue
Life Stage
Adult
Tissue
Brain
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

This lysate was produced from total protein - Human Adult Normal Tissue: Brain. Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Brain Lysate (NB820-59177)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA, ELISPOT, IP, WB.


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ELISA
(2)
ELISPOT
(1)
IP
(1)
WB
(3)
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Human
(3)
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Showing Publications 1 - 8 of 8.
Publications using NB820-59177 Applications Species
Majumder P, Roy K, Singh BK et al. Cellular levels of growth factor receptor bound protein 2 (Grb2) and cytoskeleton stability are correlated in a neurodegenerative scenario. Dis Model Mech. Mar 30 2017 [PMID: 28360125] (WB) WB
Saito K, Ohta S, Kawakami Y et al. Functional analysis of KIF20A, a potential immunotherapeutic target for glioma. J Neurooncol 2017 Mar [PMID: 28070829]
Wunsch M, Hohmann C, Milles B et al. The Correlation between the Virus- and Brain Antigen-Specific B Cell Response in the Blood of Patients with Multiple Sclerosis. Viruses. Apr 28 2016 [PMID: 27120609] (ELISA) ELISA
Rasmussen RD, Gajjar MK, Jensen KE et al. Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma. Mol Oncol. 2016 Jan 08 [PMID: 26794465] (WB) WB
Choi I, Kim B, Byun JW et al. LRRK2 G2019S mutation attenuates microglial motility by inhibiting focal adhesion kinase. Nat Commun 2015 Sep 14 [PMID: 26365310] (IP) IP
Molina J, Rodriguez-Diaz R, Fachado A et al. Control of insulin secretion by cholinergic signaling in the human pancreatic islet. Diabetes 2014 Aug [PMID: 24658304] (WB, Human) WB Human
Kuerten S, Pommerschein G, Barth SK et al. Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood. Clin. Immunol. 3/5/2014 [PMID: 24607792] (ELISPOT, Human) ELISPOT Human
Hohmann C, Milles B, Schinke M, Schroeter M. Categorization of multiple sclerosis relapse subtypes by B cell profiling in the blood. Acta Neuropathologica Communications. 2014 Sep 16 (ELISA, Human) ELISA Human

Review for Brain Lysate (NB820-59177) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB820-59177:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Brain NB820-59177
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reviewed by:
Ming Lu
WB Human 10/03/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman Brain Whole Tissue Lysate (Adult Normal), Novus NB820-59177
SpeciesHuman
LotA906100
CommentsNovus is quite generous to give 1 mg of Human Brain Whole Tissue Lysate (Adult Normal) for this item, which for sure will last a long time for western blot. The quality of the lysate is quite good.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution RatioNovus b-actin (NBP1-47423) 1:1000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Novusbio Cat#NB7539
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives strong band at correct MW.

Comments

CommentsNovus is quite generous to give 1 mg of Human Brain Whole Tissue Lysate (Adult Normal) for this item, which for sure will last a long time for western blot. The quality of the lysate is quite good.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Brain Lysate (NB820-59177). (Showing 1 - 5 of 5 FAQs).

  1. We are seeking a normal brain tissue lysate for control of Parkinson’s disease. In your website, there is Human Brain Parietal Lobe Whole Tissue Lysate (Adult Parkinson's) 1.0mg (NB820-59414). If you have any normal brain tissue lysate, please let me know.
    • Please see this link to all of our normal human brain tissue lysates.
  2. I have a question regarding your product no. NB820-59177. I would like to use this lysate in an ELISPOT assay and I am not sure if this is possible. My particular question is if this product is sterile, i.e. can it be used for cell culture?
    • Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.
  3. May we ask how much we shall load by using NB820-59177 as positive control in WB?
    • We recommend loading 10-20ug of lysate per well for Western blot.
  4. Is there a recommended expiration date for the Human Brain Whole Tissue Lysate, cat:NB820-59177, Lot: B703069?
    • You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.
  5. May we ask if the Human Brain Whole Tissue Lysate (cat. no. NB820-59177) is suitable for RNA extraction?
    • Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.

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Recent Reviews

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Ming Lu
10/03/2014
Application: WB
Species: Human