Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer ...read more
FAQs for Brain Lysate (NB820-59177). (Showing 1 - 5 of 5 FAQs).
We are seeking a normal brain tissue lysate for control of Parkinson’s disease. In your website, there is Human Brain Parietal Lobe Whole Tissue Lysate (Adult Parkinson's) 1.0mg (NB820-59414). If you have any normal brain tissue lysate, please let me know.
Please see this link to all of our normal human brain tissue lysates.
I have a question regarding your product no. NB820-59177. I would like to use this lysate in an ELISPOT assay and I am not sure if this is possible. My particular question is if this product is sterile, i.e. can it be used for cell culture?
Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.
May we ask how much we shall load by using NB820-59177 as positive control in WB?
We recommend loading 10-20ug of lysate per well for Western blot.
Is there a recommended expiration date for the Human Brain Whole Tissue Lysate, cat:NB820-59177, Lot: B703069?
You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.
May we ask if the Human Brain Whole Tissue Lysate (cat. no. NB820-59177) is suitable for RNA extraction?
Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.