LC3A Products

Antibodies
LC3A Antibody
LC3A Antibody
NBP1-19167
Species: Hu, Mu, Rt, Bv, Ze
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Host: Rabbit Polyclonal
Conjugate Catalog # Availability Size Price
ELISA Kits
Human LC3A DuoSet ELISA, 5 Pl ...
Human LC3A DuoSet ELISA, 5 Plate
DY8558-05
Species: Hu
Applications: ELISA
Lysates
LC3A Overexpression Lysate
LC3A Overexpression Lysate
NBP2-04906
Species: Hu
Applications: WB
Proteins
Recombinant Human LC3A GST (N ...
Recombinant Human LC3A GST (N-Term...
H00084557-P01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, PA, PAGE, AP
Recombinant Human LC3A Agaros ...
Recombinant Human LC3A Agarose Pro...
UL-435
Species: Hu
Applications: Enzyme Activity
Conjugate Catalog # Availability Size Price
Formulation Catalog # Availability Price  
Recombinant Human HA-LC3A Vin ...
Recombinant Human HA-LC3A Vinyl Su...
UL-451
Species: Hu
Applications: Enzyme Activity
Conjugate Catalog # Availability Size Price
Formulation Catalog # Availability Price  

Description

Human Microtubule-associated Protein 1A/1B Light Chain 3A (MAP1LC3A), also called LC3A for short, is a 121 amino acid (aa) protein with a theoretic molecular weight of ~14 kDa. LC3A belongs to the LC3 subfamily of Autophagy-related 8 (Atg8) proteins, which also includes LC3B and LC3C (1). The process of autophagy is the bulk degradation of proteins and organelles. Whether these three proteins have distinct roles in autophagy remains unclear, but they do have unique subcellular expression (2). Specifically, LC3A shows perinuclear and nuclear localization (2). The Atg8 family members share a similar structure of two amino-terminal alpha-helices and a ubiquitin-like core but are unique in aa sequence (1). LC3 utilizes a ubiquitin-like conjugation system to covalently bind to phosphatidylethanolamine (PE), also called lipidation, which is mediated by a series of steps (1, 3). Briefly, unprocessed, cytosolic LC3 (pro-LC3) is cleaved by the cysteine protease Atg4 to expose a c-terminal glycine (Gly) residue (LC3-I); the Gly is activated by E1-like enzyme Atg7, transferred to E2-like enzyme Atg3, and an E3-like complex facilitates the conjugation of LC3 with PE (LC3-II), incorporating it into the phagophore membrane during autophagosome formation (1, 3). The recruitment of LC3 to the phagophore is thought to mediate membrane elongation and closure (1, 3). Additionally, LC3 plays a role in recruiting cargo (protein aggregates, pathogens, and organelles) to autophagosomes and delivery for lysosomal degradation (1).

The process of autophagy is associated with a variety of diseases including neurodegenerative diseases, neuromuscular, tumorigenesis, and viral and bacterial infections (4). LC3 is a useful marker of autophagy in both healthy and diseased cells (4). Interestingly, LC3A has two variants (v1 and v2) which differ in N-terminal sequence due to the varying transcriptional start sites (5). One particular study found that LC3Av1, but not v2 or LC3B, was silenced in various cancer cell lines due to aberrant DNA methylation and re-expression of LC3Av1 in LC3Av1-silenced cells inhibited tumor growth, where overall findings suggest a possible tumor-suppressive role (5).

Alternative names for LC3A include Apg8, APG8a, ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1A/1B light chain 3 A, microtubule-associated proteins 1A/1B light chain 3, and MLP3A.

References

1. Shpilka, T., Weidberg, H., Pietrokovski, S., & Elazar, Z. (2011). Atg8: an autophagy-related ubiquitin-like protein family. Genome biology. https://doi.org/10.1186/gb-2011-12-7-226

2. Koukourakis, M. I., Kalamida, D., Giatromanolaki, A., Zois, C. E., Sivridis, E., Pouliliou, S., Mitrakas, A., Gatter, K. C., & Harris, A. L. (2015). Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines. PloS one. https://doi.org/10.1371/journal.pone.0137675

3. Weidberg, H., Shvets, E., & Elazar, Z. (2011). Biogenesis and cargo selectivity of autophagosomes. Annual review of biochemistry. https://doi.org/10.1146/annurev-biochem-052709-094552

4. Tanida, I., Ueno, T., & Kominami, E. (2004). LC3 conjugation system in mammalian autophagy. The international journal of biochemistry & cell biology. https://doi.org/10.1016/j.biocel.2004.05.009

5. Schaaf, M. B., Keulers, T. G., Vooijs, M. A., & Rouschop, K. M. (2016). LC3/GABARAP family proteins: autophagy-(un)related functions. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. https://doi.org/10.1096/fj.201600698R

Bioinformatics

Entrez Human
Human
Mouse
Mouse
Rat
Rat
Uniprot Human
Human
Human
Human
Human
Human
Human
Human
Human
Product By Gene ID 84557
Alternate Names
  • MAP1A/MAP1B LC3 A
  • MAP1A/MAP1B light chain 3 A
  • MAP1BLC3
  • Microtubule-associated protein 1 light chain 3 alpha
  • MAP1 light chain 3-like protein 1
  • MAP1ALC3
  • MAP1LC3A
  • Autophagy-related ubiquitin-like modifier LC3 A
  • microtubule-associated proteins 1A/1B light chain 3A
  • Autophagy-related protein LC3 A
  • microtubule-associated proteins 1A/1B light chain 3
  • LC3A
  • MAP1A/1B light chain 3 A
  • LC3
  • ATG8E

Research Areas for LC3A

Find related products by research area and learn more about each of the different research areas below.

Autophagy
Cancer
Cellular Markers
Cytoskeleton Markers
Hypoxia
Neuroscience
Phospho-Specific
Signal Transduction

PTMs for LC3A

Learn more about PTMs related to LC3A.

Cleavage
Phosphorylation
Methylation
Ubiquitination

Bioinformatics Tool for LC3A

Discover related pathways, diseases and genes to LC3A. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
 
Vizit™, under license from BioVista Inc.

Related LC3A Blog Posts

Check out the latest blog posts on LC3A.
The LC3 A, B, C’s and 1, 2, 3’s
By Christina Towers, PhD Autophagy is a catabolic process used to breakdown and recycle damaged proteins and organelles. It is a multistep process that, in its simplest form, consists of 4 steps: initiation, phago...    Read more.
Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...    Read more.
Nuclear LC3: Why is it there and what is it doing?
By Christina Towers, PhD. Cells use the complex process of autophagy to degrade and recycle cytoplasmic material.  There are over 20 proteins that have been implicated in this process and appropriately named core...    Read more.
Why LC3B Antibodies Make Ideal Autophagosomes Membrane Markers
The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants; LC3A, LC3B and LC3C (He et al., 2003). LC3B is a subunit of the MAP1A and MAP1B microtubule-binding pr...    Read more.
Read more LC3A related blogs.