Beclin 1 Antibody

Images

 
Western Blot: Beclin 1/ATG6 Antibody [NB500-249] - WB analysis of Belclin1. Lane 1 human brain and Lane 2 mouse brain.
Immunocytochemistry/ Immunofluorescence: Beclin 1/ATG6 Antibody [NB500-249] - Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: Beclin 1/ATG6 Antibody [NB500-249] - ATG6 on pheochromocytes of the Adrenal Medulla 40x.
Western Blot: Beclin 1/ATG6 Antibody [NB500-249] - Detection of Beclin 1 in mouse liver tissue lysate (50 ug) using NB500-249. ECL detection at 1 minute.
Immunohistochemistry-Paraffin: Beclin 1/ATG6 Antibody [NB500-249] - Analysis of Beclin1 in mouse kidney. Image courtsey of product review submitted by Kelly Hudkins.
Simple Western: Beclin 1/ATG6 Antibody [NB500-249] - Simple Western lane view shows a specific band for Beclin1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Beclin 1 Antibody Summary

Immunogen
Internal synthetic peptide to human Beclin 1, within residues 1-100 [UniProt# Q14457].
Localization
Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BECN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 1:80
Application Notes
This Beclin 1 Antibody is useful for Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry paraffin-embedded sections. In Western Blot, a band is seen at ~52 kDa representing Beclin 1.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Beclin 1 Lysate (NBL1-07966)
Control Peptide
Beclin 1 Peptide (NB500-249PEP)
Reviewed Applications
Read 5 Reviews rated 4.2
using
NB500-249 in the following applications:

Publications
Read Publications using
NB500-249 in the following applications:

  • 7 publications
  • IHC
    5 publications
  • 5 publications
  • IP
    4 publications
  • WB
    50 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Beclin 1 Antibody

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • Beclin 1
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • BECN1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30

Background

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-15071
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NB500-249
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for Beclin 1 Antibody (NB500-249)(74)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(7)
IHC
(5)
IHC-P
(5)
IP
(4)
WB
(50)
All Applications
Filter By Species
Human
(33)
Mouse
(21)
Rat
(7)
All Species
Showing Publications 1 - 10 of 74. Show All 74 Publications.
Publications using NB500-249 Applications Species
Ouyang YB, Ning S, Adler JR et al. Alteration of Interneuron Immunoreactivity and Autophagic Activity in Rat Hippocampus after Single High-Dose Whole-Brain Irradiation. Cureus 2017 Jun 30 [PMID: 28861331] (ICC/IF, Rat) ICC/IF Rat
Wang CY, Hong YH, Syu JS et al. LRWD1 Regulates Microtubule Nucleation and Proper Cell Cycle Progression in the Human Testicular Embryonic Carcinoma Cells. J. Cell. Biochem. 2017 Jun 01 [PMID: 28569402]
Gao Y, Zhuang Z, Gao S et al. Tetrahydrocurcumin reduces oxidative stress-induced apoptosis via the mitochondrial apoptotic pathway by modulating autophagy in rats after traumatic brain injury. Am J Transl Res 2017 Mar 15 [PMID: 28386319] (WB, Rat) WB Rat
Gelmetti V, DeRosa P, Torosantucci L et al. PINK1 and BECN1 relocalize at mitochondria-associated membranes during mitophagy and promote ER-mitochondria tethering and autophagosome formation. Autophagy. Dec 23 2016 [PMID: 28368777] (ICC/IF, WB, IP, Human) ICC/IF, WB, IP Human
Huang W, Ao P, Li J et al. Autophagy Protects Advanced Glycation End Product-Induced Apoptosis and Expression of MMP-3 and MMP-13 in Rat Chondrocytes. Biomed Res Int. [PMID: 28265573] (WB, Rat) WB Rat
Eritja N, Chen BJ, Rodriguez-Barrueco R et al. Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer Autophagy Jan 5 2017 [PMID: 28055301] (WB, Human) WB Human
Ugun-Klusek A, Tatham MH, Elkharaz J et al. Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway Cell Death Dis Jan 5 2017 [PMID: 28055010] (IHC, Mouse) IHC Mouse
Yang Y, Guo C, Liao B et al. BAMBI inhibits inflammation through the activation of autophagy in experimental spinal cord injury Int. J. Mol. Med Feb 1 2017 [PMID: 28035406] (Rat) Rat
Jang JE, Eom JI, Jeung HK et al. AMPK-ULK1-mediated autophagy confers resistance to BET inhibitor JQ1 in acute myeloid leukemia stem cells. Clin. Cancer Res. Nov 18 2016 [PMID: 27864418] (WB, Human) WB Human
Hu YB, Zou Y, Huang Y et al. ROCK1 Is Associated with Alzheimer's Disease-Specific Plaques, as well as Enhances Autophagosome Formation But not Autophagic Abeta Clearance. Front Cell Neurosci. Nov 17 2016 [PMID: 27853422] (IHC, Human) IHC Human
Show All 74 Publications.

Reviews for Beclin 1 Antibody (NB500-249) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Rat.
We have 5 reviews tested in 3 application: WB, IHC-P, IHC.

Reviews using NB500-249:
Filter by Applications
WB
(2)
IHC-P
(2)
IHC
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Beclin 1 NB500-249
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reviewed by:
Anonymous
WB Rat 01/18/2017
View

Summary

ApplicationWestern Blot
Sample TestedChondrocyte cell lysate
SpeciesRat
Immunohistochemistry-Paraffin Beclin 1 NB500-249
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reviewed by:
Ashwini Katre
IHC-P Human 03/05/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman ovarian carcinoma -patient derived xenograft
SpeciesHuman
LotK-2

Blocking

Blocking Details10% goat serum room temp 1 hour

Primary Anitbody

Dilution Ratio1:400 in 10% goat serum overnight at 4degrees

Secondary Antibody

Secondary Descriptiongoat anti rabbit HRP polymer from vector labs
Secondary Manufacturer Cat#MP-7401

Details

Detection NotesDAB for 3 min
Fixation DetailsAntigen retrieval in pH6 citrate buffer pressure cook for 4 min high pressure
Wash Description1XPBS 3minX3
Immunohistochemistry-Paraffin Beclin 1 NB500-249
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 10/08/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse kidney
SpeciesMouse
LotJ
CommentsThis antibody gave robust staining with low background.

Blocking

Blocking Details2.5% Normal horse serum, 30 minutes, room temperature

Primary Anitbody

Dilution Ratio1:500 in PBS + 1% BSA, overnight at 4C

Secondary Antibody

Secondary DescriptionImmPress Rabbit HRP
Secondary Manufacturer Cat#Vector Labs # MP 7401
Secondary ConcentrationRTU

Details

Detection NotesQuanto DAB (Fisher), 1 minute; N Rb IgG negative control
Fixation DetailsFFPE, heat mediated antigen retrieval in citrate buffer pH 6
Wash DescriptionTBS/Tween, 2 x 10 minutes

Comments

CommentsThis antibody gave robust staining with low background.
 
reviewed by:
Nellie Reuland
WB Mouse 10/06/2012
View

Summary

ApplicationWestern Blot
Sample TestedMouse whole cell lysate
SpeciesMouse
CommentsIn general, the antibody works very well at low concentrations. However, there are two bands near where beclin1 is predicted to fall given its molecular weight. Although I have SOME confidence choosing the right one, if I were to repeat this experiment, I'd want to do so with a positive control to verify my band. Additionally, I may repeat with a higher primary antibody concentration.

Blocking

Blocking Details2 hours at room temperature with SuperBlock (Pierce propietary solution)

Primary Anitbody

Dilution Ratio1:1000 in superblock. overnight at 4C

Secondary Antibody

Secondary DescriptionGoat antirabbit IgG-HRP
Secondary Manufacturer Cat#sc2004
Secondary Concentration1:5000 for 1 hour at RT

Details

Detection NotesHRP with chemiluminescent detection, using UVP software. 8 minute exposure

Comments

CommentsIn general, the antibody works very well at low concentrations. However, there are two bands near where beclin1 is predicted to fall given its molecular weight. Although I have SOME confidence choosing the right one, if I were to repeat this experiment, I'd want to do so with a positive control to verify my band. Additionally, I may repeat with a higher primary antibody concentration.
 
reviewed by:
Anonymous
IHC Human 09/28/2010
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman Esophagus tissue
SpeciesHuman

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1500

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:1500

Details

Detection NotesDetection Visualization Method: DAB
Fixation DetailsSample Preparation: FFPE

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Beclin 1 Antibody (NB500-249). (Showing 1 - 9 of 9 FAQs).

  1. I am interested in Novus' Beclin 1 peptide NB500-249PEP. Would you please send me the sequence information, and any data you may have for this peptide?
    • In regards to your inquiry, this Beclin 1 peptide is useful as a blocking peptide to our primary antibody NB500-249. This peptide contains amino acids that fall within the range of 1-100. Unfortunately the exact sequence is deemed proprietary by the lab.
  2. I'm looking for a Beclin 1 antibody, which do you recommend for use in western blot?
    • Our best selling beclin 1 antibody NB500-249, has been cited in 41 publications where it was used in western blot. Additionally this beclin 1 antibody is typically available for immediate shipment.
  3. I've had good results with your beclin antibody in IHC but now I need a beclin one antibody to use in ELISA, will this beclin antibody also work as a capture antibody in ELISA?
    • This particular beclin 1 antibody has not yet been validated for use in ELISA, we do however have 2 beclin 1 antibodies that are validated in ELISA, NBP2-22176 and NBP1-76648. Alternatively you could take advantage of our innovator's reward program which would allow you to test this beclin 1 antibody in ELISA with out financial risk. Just purchase the beclin antibody and test in ELISA, share your results (negative or positive) and we will issue a voucher for the purchase price of the antibody.
  4. Is your belcin 1 antibody NB500-249 available as an atto 488 direct conjugate?
    • Unfortunately we do not offer Atto conjugates but we do offer this beclin 1 antibody as an AlexaFlour 488 direct conjugate and as a DyLight 488 conjugated antibody.
  5. Can a Lightning-Link HRP labeling kit be used to label the beclin 1 antibody NB500-249, I've used it to label mouse isotypes but wasn't sure if it will work on a rabbit IgG antibody like this beclin antibody?
    • I can't find any specific mention of this beclin 1 antibody being labeled with a lightning link kit; however lightning link kits are extreamly versatile in terms of labeling ability and should be able to label this beclin 1 antibody without any trouble.
  6. I'm using the ATDC5 murine carcinoma cell line, has beclin 1 antibody been documented as working in this cell line?
    • From a cursory look through our publication list I don't see that this beclin 1 antibody has been published as being used in that cell line. We show this beclin 1 antibody was published 19 times as being used in mouse, if I can get your contact info I will take a more in depth look to see if the beclin 1 antibody has been used in that cell line.
  7. I'm doning an autophagy study using CHO-K1 cells, do you know if this beclin 1 antibody will detect beclin in that cell line?
    • Based on tissue expression data from protein atlas beclin 1 is not highly expressed in ovarian tissue; however in ovarian cancer tissues beclin 1 expression is much greater. We don't have any beclin 1 antibodies that have been validated for use in hamster so you may want to take advantage of our innovator's reward program.
  8. The beclin 1 antibody NB500-249 has good data but I need a beclin atibody that will work with bovine tissue but I don't see bovine listed as reactive with the beclin 1 antibody. Is bovine not listed because you know this beclin one antibody will not recognize?
    • Although reactivity with bovine has not been experimentally validated, a comparison of the human and bovine sequences within the immunogen range indicates that cross reactivity is likely. An alignment of the human beclin 1 sequence and bovine beclin 1 sequence shows there is 95% match in the region that was used as the immunogen.
  9. Will this beclin 1 antibody detect beclin that is cleaved at the last 50 aa of the c-terminal?
    • Because the immunogen used to make this beclin 1 antibody falls within the first 100 amino acids of the N-terminal it should detect beclin 1 that is cleaved at the c-terminal.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-249AF405
Alexa Fluor 488 Labeled NB500-249AF488
Alexa Fluor 647 Labeled NB500-249AF647
Alexa Fluor 700 Labeled NB500-249AF700
Biotin Labeled NB500-249B
DyLight 405 Labeled NB500-249V
DyLight 488 Labeled NB500-249G
DyLight 550 Labeled NB500-249R
DyLight 650 Labeled NB500-249C
FITC Labeled NB500-249F
HRP Labeled NB500-249H

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Blogs on Beclin 1.

Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily...  Read full blog post.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

CHOP/GADD153 - A regulator and marker for ER-stress induced apoptosis
C/EBP homologous protein (CHOP) is a transcription factor that regulates apoptosis in response to cellular stress. CHOP also known as growth arrest and DNA damage 153 (GADD153) was first cloned because of its induction in response to genotoxic str...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

Wide Ranging Uses for the Autophagy Marker - Beclin-1 Antibody
Beclin 1 is the first mammalian gene identified as a mediator of autophagy, and plays important roles in development, tumorigenesis, and neurodegeneration. Research with the beclin-1 antibody has revealed that, Beclin 1 is found in complex with the th...  Read full blog post.

Beclin 1: Regulator of Autophagy and Apoptosis
Beclin 1 is the mammalian orthologue of the yeast Apg6/Vps30 gene. Beclin 1 can complement the defect in autophagy present in apg6 yeast strains and stimulate autophagy when overexpressed in mammalian cells (1) and can bind to Bcl2, an important regul...  Read full blog post.

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Recent Reviews

4.2
5
2
4
2
3
1
2
0
1
0

Anonymous
01/18/2017
Application: WB
Species: Rat

Ashwini Katre
03/05/2014
Application: IHC-P
Species: Human

Anonymous
10/08/2012
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol BECN1
Entrez