Beclin 1 Antibody


Western Blot: Beclin 1/ATG6 Antibody [NB500-249] - WB analysis of Belclin1. Lane 1 human brain and Lane 2 mouse brain.
Immunocytochemistry/ Immunofluorescence: Beclin 1/ATG6 Antibody [NB500-249] - Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and more
Immunohistochemistry: Beclin 1/ATG6 Antibody [NB500-249] - ATG6 on pheochromocytes of the Adrenal Medulla 40x.
Western Blot: Beclin 1/ATG6 Antibody [NB500-249] - Detection of Beclin 1 in mouse liver tissue lysate (50 ug) using NB500-249. ECL detection at 1 minute.
Immunohistochemistry-Paraffin: Beclin 1/ATG6 Antibody [NB500-249] - Analysis of Beclin1 in mouse kidney. Image courtsey of product review submitted by Kelly Hudkins.
Simple Western: Beclin 1/ATG6 Antibody [NB500-249] - Simple Western lane view shows a specific band for Beclin1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

Beclin 1 Antibody Summary

Internal synthetic peptide to human Beclin 1, within residues 1-100 [UniProt# Q14457].
Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS pH 7.4
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
This Beclin 1 Antibody is useful for Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry paraffin-embedded sections. In Western Blot, a band is seen at ~52 kDa representing Beclin 1.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control Peptide
Beclin 1 Peptide (NB500-249PEP)
Reviewed Applications
Read 4 Reviews rated 4
NB500-249 in the following application:

Read Publications using
NB500-249 in the following applications:

  • 5 publications
  • IHC
    3 publications
  • 5 publications
  • IP
    3 publications
  • WB
    41 publications

Alternate Names for Beclin 1 Antibody

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • BECN1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30


Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Beclin 1 Antibody (NB500-249)(60)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using NB500-249 Applications Species
Kivinen N, Felszeghy S, Kinnunen AI et al. Absence of collagen XVIII in mice causes age-related insufficiency in retinal pigment epithelium proteostasis. Biogerontology. Apr 28 2016 12:00AM [PMID:27125427] (IHC-P, Mouse) IHC-P Mouse
Choudhury KR, Bucha S, Baksi S et al. Chaperone-like protein HYPK and its interacting partners augment autophagy. Eur. J. Cell Biol. 2016 Apr 01 [PMID:27067261] (WB, Mouse) WB Mouse
Ueno T, Saji S, Sugimoto M et al. Clinical significance of the expression of autophagy-associated marker, beclin 1, in breast cancer patients who received neoadjuvant endocrine therapy. BMC Cancer. 2016 Mar 17 [PMID:26984766] (IHC-P, Human) IHC-P Human
Masuda GO, Yashiro M, Kitayama K et al. Clinicopathological Correlations of Autophagy-related Proteins LC3, Beclin 1 and p62 in Gastric Cancer. Anticancer Res. 2016 Jan 01 [PMID:26722036] (IHC-P, Human) IHC-P Human
Park YS, Park JH, Ko J et al. mTOR inhibition by rapamycin protects against deltamethrin-induced apoptosis in PC12 Cells. Environ. Toxicol. 2015 Nov 21 [PMID:26588882] (WB, Rat) WB Rat
Majumder P, Chakrabarti O. Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101. Cell Death Dis. 2015 Nov 06 [PMID:26539917] (WB, Human) WB Human
Liu L, Li CJ, Lu Y et al. Baclofen mediates neuroprotection on hippocampal CA1 pyramidal cells through the regulation of autophagy under chronic cerebral hypoperfusion. Sci Rep 2015 [PMID:26412641] (WB) WB
Tanji K, Miki Y, Maruyama A et al. Trehalose intake induces chaperone molecules along with autophagy in a mouse model of Lewy body disease. Biochem. Biophys. Res. Commun. 2015 Aug 20 [PMID:26299928] (WB, Mouse) WB Mouse
Siddiqui MA, Mukherjee S, Manivannan P, Malathi K. RNase L Cleavage Products Promote Switch from Autophagy to Apoptosis by Caspase-Mediated Cleavage of Beclin-1. Int J Mol Sci. 2015 Aug 12 [PMID:26263979] (WB, Human) WB Human
Dever SM, Rodriguez M, Lapierre J et al. Differing roles of autophagy in HIV-associated neurocognitive impairment and encephalitis with implications for morphine co-exposure. Front Microbiol 2015 [PMID:26217309] (WB) WB
Show All 60 Publications.

Reviews for Beclin 1 Antibody (NB500-249) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 reviews tested in 3 application: IHC-P, WB, IHC.

Reviews using NB500-249:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Beclin 1 NB500-249
reviewed by:
IHC-P Human 03/05/2014


Sample TestedHuman ovarian carcinoma -patient derived xenograft


Blocking Details10% goat serum room temp 1 hour

Primary Anitbody

Dilution Ratio1:400 in 10% goat serum overnight at 4degrees

Secondary Antibody

Secondary Descriptiongoat anti rabbit HRP polymer from vector labs
Secondary Manufacturer Cat#MP-7401


Detection NotesDAB for 3 min
Fixation DetailsAntigen retrieval in pH6 citrate buffer pressure cook for 4 min high pressure
Wash Description1XPBS 3minX3
Immunohistochemistry-Paraffin Beclin 1 NB500-249
reviewed by:
IHC-P Mouse 10/08/2012


Sample TestedMouse kidney
CommentsThis antibody gave robust staining with low background.


Blocking Details2.5% Normal horse serum, 30 minutes, room temperature

Primary Anitbody

Dilution Ratio1:500 in PBS + 1% BSA, overnight at 4C

Secondary Antibody

Secondary DescriptionImmPress Rabbit HRP
Secondary Manufacturer Cat#Vector Labs # MP 7401
Secondary ConcentrationRTU


Detection NotesQuanto DAB (Fisher), 1 minute; N Rb IgG negative control
Fixation DetailsFFPE, heat mediated antigen retrieval in citrate buffer pH 6
Wash DescriptionTBS/Tween, 2 x 10 minutes


CommentsThis antibody gave robust staining with low background.
reviewed by:
WB Mouse 10/06/2012


ApplicationWestern Blot
Sample TestedMouse whole cell lysate
CommentsIn general, the antibody works very well at low concentrations. However, there are two bands near where beclin1 is predicted to fall given its molecular weight. Although I have SOME confidence choosing the right one, if I were to repeat this experiment, I'd want to do so with a positive control to verify my band. Additionally, I may repeat with a higher primary antibody concentration.


Blocking Details2 hours at room temperature with SuperBlock (Pierce propietary solution)

Primary Anitbody

Dilution Ratio1:1000 in superblock. overnight at 4C

Secondary Antibody

Secondary DescriptionGoat antirabbit IgG-HRP
Secondary Manufacturer Cat#sc2004
Secondary Concentration1:5000 for 1 hour at RT


Detection NotesHRP with chemiluminescent detection, using UVP software. 8 minute exposure


CommentsIn general, the antibody works very well at low concentrations. However, there are two bands near where beclin1 is predicted to fall given its molecular weight. Although I have SOME confidence choosing the right one, if I were to repeat this experiment, I'd want to do so with a positive control to verify my band. Additionally, I may repeat with a higher primary antibody concentration.
reviewed by:
IHC Human 09/28/2010


Sample TestedHuman Esophagus tissue

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1500

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:1500


Detection NotesDetection Visualization Method: DAB
Fixation DetailsSample Preparation: FFPE

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Beclin 1 Antibody (NB500-249). (Showing 1 - 9 of 9 FAQs).

  1. I am interested in Novus' Beclin 1 peptide NB500-249PEP. Would you please send me the sequence information, and any data you may have for this peptide?
    • In regards to your inquiry, this Beclin 1 peptide is useful as a blocking peptide to our primary antibody NB500-249. This peptide contains amino acids that fall within the range of 1-100. Unfortunately the exact sequence is deemed proprietary by the lab.
  2. I'm looking for a Beclin 1 antibody, which do you recommend for use in western blot?
    • Our best selling beclin 1 antibody NB500-249, has been cited in 41 publications where it was used in western blot. Additionally this beclin 1 antibody is typically available for immediate shipment.
  3. I've had good results with your beclin antibody in IHC but now I need a beclin one antibody to use in ELISA, will this beclin antibody also work as a capture antibody in ELISA?
    • This particular beclin 1 antibody has not yet been validated for use in ELISA, we do however have 2 beclin 1 antibodies that are validated in ELISA, NBP2-22176 and NBP1-76648. Alternatively you could take advantage of our innovator's reward program which would allow you to test this beclin 1 antibody in ELISA with out financial risk. Just purchase the beclin antibody and test in ELISA, share your results (negative or positive) and we will issue a voucher for the purchase price of the antibody.
  4. Is your belcin 1 antibody NB500-249 available as an atto 488 direct conjugate?
    • Unfortunately we do not offer Atto conjugates but we do offer this beclin 1 antibody as an AlexaFlour 488 direct conjugate and as a DyLight 488 conjugated antibody.
  5. Can a Lightning-Link HRP labeling kit be used to label the beclin 1 antibody NB500-249, I've used it to label mouse isotypes but wasn't sure if it will work on a rabbit IgG antibody like this beclin antibody?
    • I can't find any specific mention of this beclin 1 antibody being labeled with a lightning link kit; however lightning link kits are extreamly versatile in terms of labeling ability and should be able to label this beclin 1 antibody without any trouble.
  6. I'm using the ATDC5 murine carcinoma cell line, has beclin 1 antibody been documented as working in this cell line?
    • From a cursory look through our publication list I don't see that this beclin 1 antibody has been published as being used in that cell line. We show this beclin 1 antibody was published 19 times as being used in mouse, if I can get your contact info I will take a more in depth look to see if the beclin 1 antibody has been used in that cell line.
  7. I'm doning an autophagy study using CHO-K1 cells, do you know if this beclin 1 antibody will detect beclin in that cell line?
    • Based on tissue expression data from protein atlas beclin 1 is not highly expressed in ovarian tissue; however in ovarian cancer tissues beclin 1 expression is much greater. We don't have any beclin 1 antibodies that have been validated for use in hamster so you may want to take advantage of our innovator's reward program.
  8. The beclin 1 antibody NB500-249 has good data but I need a beclin atibody that will work with bovine tissue but I don't see bovine listed as reactive with the beclin 1 antibody. Is bovine not listed because you know this beclin one antibody will not recognize?
    • Although reactivity with bovine has not been experimentally validated, a comparison of the human and bovine sequences within the immunogen range indicates that cross reactivity is likely. An alignment of the human beclin 1 sequence and bovine beclin 1 sequence shows there is 95% match in the region that was used as the immunogen.
  9. Will this beclin 1 antibody detect beclin that is cleaved at the last 50 aa of the c-terminal?
    • Because the immunogen used to make this beclin 1 antibody falls within the first 100 amino acids of the N-terminal it should detect beclin 1 that is cleaved at the c-terminal.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB500-249AF405
Alexa Fluor (R) 488 Labeled NB500-249AF488
Alexa Fluor (R) 647 Labeled NB500-249AF647
Alexa Fluor (R) 700 Labeled NB500-249AF700
Biotin Labeled NB500-249B
DyLight 405 Labeled NB500-249V
DyLight 488 Labeled NB500-249G
DyLight 550 Labeled NB500-249R
DyLight 650 Labeled NB500-249C
FITC Labeled NB500-249F
HRP Labeled NB500-249H

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Blogs on Beclin 1.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

CHOP/GADD153 - A regulator and marker for ER-stress induced apoptosis
C/EBP homologous protein (CHOP) is a transcription factor that regulates apoptosis in response to cellular stress. CHOP also known as growth arrest and DNA damage 153 (GADD153) was first cloned because of its induction in response to genotoxic str...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

Wide Ranging Uses for the Autophagy Marker - Beclin-1 Antibody
Beclin 1 is the first mammalian gene identified as a mediator of autophagy, and plays important roles in development, tumorigenesis, and neurodegeneration. Research with the beclin-1 antibody has revealed that, Beclin 1 is found in complex with the th...  Read full blog post.

Beclin 1: Regulator of Autophagy and Apoptosis
Beclin 1 is the mammalian orthologue of the yeast Apg6/Vps30 gene. Beclin 1 can complement the defect in autophagy present in apg6 yeast strains and stimulate autophagy when overexpressed in mammalian cells (1) and can bind to Bcl2, an important regul...  Read full blog post.

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Product PDFs


Gene Symbol BECN1

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