Beclin 1 Antibody - BSA Free

Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249] - Detection of Beclin 1 (red) in Pheochromocytes of the Adrenal Medulla 40x.

Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on U-87MG cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Representative Western blot images and quantitative analyses of Beclin 1 from the left hemisphere of rat brains at different time points after SAH. Sample size is 36, n = 6 per group. Data were presented as mean +/- SD. F = 12.37 for Beclin 1. *P < .05, ** P < .01, ***P < .001 vs Sham group. SAH, subarachnoid hemorrhage. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31436915/) licensed under a CC-BY licence.

Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Analysis of Belclin1. Lane 1: human brain. Lane 2: mouse brain.

Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Analysis of Beclin1 in mouse kidney. Image courtsey of product review submitted by Kelly Hudkins.

Immunohistochemistry-Frozen: Beclin 1 Antibody - BSA Free [NB500-249] - Merged immunostaining of frozen section of Rat brain tissue. Image from verified customer review.

Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on HeLa cells with NB500-249AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on HepG2 cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on THP-1 cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on Neuro2a cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Simple Western: Beclin 1 Antibody - BSA Free [NB500-249] - Image shows a specific band for Beclin1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Knockdown Validated: Beclin 1 Antibody - BSA Free [NB500-249] - siRNA knockdown of becn1 (Beclin 1). Lysates from OKP7 cells treated with non-targeting siRNA (non-target) or an siRNA pool directed against Beclin 1 (becn1) were analyzed by Western blot for Beclin 1 production. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1003394) licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Beclin 1 Antibody - BSA Free Summary

• Immunogen: Internal synthetic peptide to human Beclin 1, within residues 1-100 [UniProt# Q14457].
• Localization: Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BECN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 1:80
  • Knockdown Validated
  • Simple Western 1:50
  • Western Blot 1:500-1:2000
• Control:

  Beclin 1 Overexpression Lysate

• Control Peptide:

  Beclin 1 Protein (NB500-249PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Beclin 1 Antibody - BSA Free

• ATG6 autophagy related 6 homolog
• ATG6
• beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
• beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
• Beclin 1
• beclin 1, autophagy related
• beclin1
• beclin-1
• BECN1
• Coiled-coil myosin-like BCL2-interacting protein
• GT197
• Protein GT197
• VPS30

Background

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Beclin 1 Antibody (NB500-249)(139)

Publications using NB500-249

• Qu W, Jeong A, Zhong R et al. Deletion of Small GTPase H-Ras Rescues Memory Deficits and Reduces Amyloid Plaque-Associated Dendritic Spine Loss in Transgenic Alzheimer's Mice Molecular neurobiology 2022-10-26 [PMID: 36287323]
• Karanasios E, Ktistakis NT. Studying Autophagy: List of Useful Antibodies Produced via a Community Effort. Autophagy at the Cell, Tissue and Organismal Level. 2016-04-24
• Lee SH, Lee JS, Park JH et al. Glycolytic Metabolic Remodeling by the Truncate of Glioma-Associated Oncogene Homolog 1 in Triple-Negative Breast Cancer Cells Journal of Cancer 2022-08-08 [PMID: 36046646] (WB, Human)
• BITGEN N, BARAN M, ONDER G et al. Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy Cukurova Medical Journal 2022-06-30 (ICC/IF)
• Karuppan MKM, Ojha CR, Rodriguez M, Lapierre J Zika virus infection during pregnancy and induced brain pathology in beclin1-deficient mouse model bioRxiv 2019-11-15 (WB, Mouse)
• GOKTEPE O, ONDER G, BOLAT D et al. Ganoderma lucidum inhibits proliferation and promotes autophagy in breast cancer cell lines with varying hormonal sensitivity by regulating Beclin-1, LC3, and p62 Cukurova Medical Journal 2022-12-28 (ICC/IF, Mouse, Human)
• Peischard S, Moller M, Disse P Et al. Virus-induced inhibition of cardiac pacemaker channel HCN4 triggers bradycardia in human-induced stem cell system Cell Mol Life Sci 2022-07-21 [PMID: 35864219] (ICC, Human)
  
  Details:
  Citation using the Alexa Fluor 350 version of this antibody.
• Gergin OO, Pehlivan SS, Ulger M et al. Efficacy of stem cell-based therapies for colistin-induced nephrotoxicity Environmental toxicology and pharmacology 2022-07-19 [PMID: 35863655]
• Zhang K, Hu Z, Ding Q et al. Long-Term Copper Exposure Induced Excessive Autophagy of the Porcine Spleen Biological trace element research 2022-07-06 [PMID: 35794302]
• Shi L, Xiao C, Zhang Y et al. Vitamin D/vitamin D receptor/Atg16L1 axis maintains podocyte autophagy and survival in diabetic kidney disease Renal failure 2022-12-01 [PMID: 35469547] (IHC, WB, Rat)
• Mandic M, Misirkic Marjanovic M, Vucicevic L et al. MAP kinase-dependent autophagy controls phorbol myristate acetate-induced macrophage differentiation of HL-60 leukemia cells Life sciences 2022-05-15 [PMID: 35304128] (IP, Human)
• Nuth M, Benakanakere MR, Ricciardi RP Discovery of a potent cytotoxic agent that promotes G2/M phase cell cycle arrest and apoptosis in a malignant human pharyngeal squamous carcinoma cell line International journal of oncology 2022-04-01 [PMID: 35211767] (WB, Human)
• Ramasubramanian B, Griffith C, Hanson M et al. Protective Effects of Chaya against Mitochondrial and Synaptic Toxicities in the Type 2 Diabetes Mouse Model TallyHO Cells 2022-02-21 [PMID: 35203393] (WB, Mouse)
• Majumder S, Pushpakumar S, Juin SK et al. Toll-like receptor 4 mutation protects the kidney from Ang-II-induced hypertensive injury Pharmacological research 2021-12-09 [PMID: 34896544] (WB, Mouse)
• Cheng Y, Kim WK, Wellman LL Et al. Short-Term Sleep Fragmentation Dysregulates Autophagy in a Brain Region-Specific Manner Life (Basel, Switzerland) 2021-10-16 [PMID: 34685469] (Mouse)
• Krunic M, Ristic B, Bosnjak M Et al. Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death Free radical biology & medicine 2021-10-20 [PMID: 34678419] (WB, Human)
• Kosic M, Paunovic V, Ristic B et al. 3-Methyladenine prevents energy stress-induced necrotic death of melanoma cells through autophagy-independent mechanisms Journal of Pharmacological Sciences 2021-09-01 [PMID: 34294367] (WB, Mouse)
• Wen X, Zhang J, Yang W et al. CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis Experimental and Therapeutic Medicine 2021-02-11 [PMID: 33732328] (WB, Mouse)
• Obert D, Wolpert A, Grimm N, Korff S ER stress preconditioning ameliorates liver damage after hemorrhagic shock and reperfusion Exp Ther Med 2021-02-19 [PMID: 33603856]
• Leist SR, Dinnon KH, SchAfer A et al. A Mouse-Adapted SARS-CoV-2 Induces Acute Lung Injury and Mortality in Standard Laboratory Mice Cell 2020-09-23 [PMID: 33031744] (WB, SARS-CoV)
• Li X, Hu Z, Shi H et al. Inhibition of VEGFA Increases the Sensitivity of Ovarian Cancer Cells to Chemotherapy by Suppressing VEGFA-Mediated Autophagy Onco Targets Ther 2020-09-05 [PMID: 32884298]
• Yefimova, M G, LefEvre, C Et al. Granulosa cells provide elimination of apoptotic oocytes through unconventional autophagy-assisted phagocytosis. Hum Reprod 2020-06-01 [PMID: 32531067] (ICC/IF, Human)
• Sacitharan P, Bou-Gharios G, Edwards J SIRT1 directly activates autophagy in human chondrocytes Cell Death Discov. 2020-12-01 [PMID: 32528730] (WB, Human)
• Chen C, Liu L, Shu YQ et al. Blockade of HCN2 Channels Provides Neuroprotection Against Ischemic Injury via Accelerating Autophagic Degradation in Hippocampal Neurons Neurosci Bull 2020-06-10 [PMID: 32519067]
• Lee JH, Parveen A, Do MH et al. Molecular mechanisms of methylglyoxal-induced aortic endothelial dysfunction in human vascular endothelial cells Cell Death Dis 2020-05-28 [PMID: 32467587]
• Dutta K, Thammisetty SS, Boutej H et al. Mitigation of ALS pathology by neuron-specific inhibition of nuclear factor kappa B signaling J. Neurosci. 2020-05-26 [PMID: 32457070] (WB, Mouse)
• Zhang R, Li SW, Liu L et al. TRIM11 facilitates chemoresistance in nasopharyngeal carcinoma by activating the beta-catenin/ABCC9 axis via p62-selective autophagic degradation of Daple Oncogenesis 2020-05-07 [PMID: 32382014] (Human)
• Cui, D, Drake, J C Et al. A novel voluntary weightlifting model in mice promotes muscle adaptation and insulin sensitivity with simultaneous enhancement of autophagy and mTOR pathway. FASEB J 2020-06-01 [PMID: 32304342] (WB, Human)
• Fedele A, Proud C Chloroquine and bafilomycin A mimic lysosomal storage disorders and impair mTORC1 signalling. Biosci Rep. 2020-04-30 [PMID: 32285908] (WB, Human)
• Zhao J, Li G, Zhao X et al. Down-regulation of AMPK signaling pathway rescues hearing loss in TFB1 transgenic mice and delays age-related hearing loss Aging (Albany NY) 2020-04-02 [PMID: 32240104] (WB, Mouse)
• Cursano S, Battaglia CR, Urrutia-Ruiz C et al. A CRHR1 antagonist prevents synaptic loss and memory deficits in a trauma-induced delirium-like syndrome Mol. Psychiatry 2020-02-12 [PMID: 32051550] (WB, Mouse)
• Urasaki, Y, Beaumont, C Et al. Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways. Nutrients 2020-01-30 [PMID: 32019055] (WB, Human)
• Gao J, Long L, Xu F et al. Icariside II, a phosphodiesterase 5 inhibitor, attenuates cerebral ischemia/reperfusion injury by inhibiting GSK-3 beta-mediated activation of autophagy Br J Pharmacol. 2019-10-28 [PMID: 31658364] (KD, WB, Rat)
  
  Details:
  Citation used the Biotin format of this antibody.
• Ognibene M, Podesta M, Garaventa A, Pezzolo A Role of GOLPH3 and TPX2 in Neuroblastoma DNA Damage Response and Cell Resistance to Chemotherapy Int J Mol Sci 2019-09-25 [PMID: 31557970] (ICC/IF, WB, Human)
• Odeh M, Tamir-Livne Y, Haas T, Bengal E P38 alpha MAPK coordinates the activities of several metabolic pathways that together induce atrophy of denervated muscles FEBS J. 2019-09-23 [PMID: 31545558]
• Sun C, Enkhjargal B, Reis C et al. Osteopontin-Enhanced Autophagy Attenuates Early Brain Injury via FAK-ERK Pathway and Improves Long-Term Outcome after Subarachnoid Hemorrhage in Rats Cells 2019-08-27 [PMID: 31461955] (ICC/IF, IHC-Fr, WB, Rat)
• Sun CM, Enkhjargal B, Reis C et al. Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats CNS Neurosci Ther 2019-08-22 [PMID: 31436915] (ICC/IF, WB, Rat)
• Tedeschi V, Petrozziello T, Sisalli MJ et al. The activation of Mucolipin TRP channel 1 (TRPML1) protects motor neurons from L-BMAA neurotoxicity by promoting autophagic clearance Sci Rep 2019-07-24 [PMID: 31341250] (WB, Mouse)
• Chai W, Ye F et al. HMGB1-mediated autophagy regulates sodium/iodide symporter protein degradation in thyroid cancer cells. J Exp Clin Cancer Res 2019-07-22 [PMID: 31331356] (WB, Human)
• Roy, BC;Ahmed, I;Ramalingam, S;Jala, V;Haribabu, B;Ramamoorthy, P;Ashcraft, J;Valentino, J;Anant, S;Sampath, V;Umar, S; Co-localization of autophagy-related protein p62 with cancer stem cell marker dclk1 may hamper dclk1's elimination during colon cancer development and progression Oncotarget 2019-03-22 [PMID: 31040926] (ICC/IF, Mouse)
• Tamrakar S, Yashiro M, Kawashima T et al. Clinicopathological Significance of Autophagy-related Proteins and its Association With Genetic Alterations in Gliomas Anticancer Res. 2019-03-01 [PMID: 30842153] (IHC, Human)
• Ueno T, Masuda N, Kamigaki S et al. Differential Involvement of Autophagy and Apoptosis in Response to Chemoendocrine and Endocrine Therapy in Breast Cancer: JBCRG-07TR Int J Mol Sci 2019-02-24 [PMID: 30813476] (IHC, Human)
• Ye B, Wang Q, et al. Restoring autophagic flux attenuates cochlear spiral ganglion neuron degeneration by promoting TFEB nuclear translocation via inhibiting MTOR. Autophagy 2019-06-01 [PMID: 30706760] (WB, Mouse)
• Houtman J, Freitag K, Gimber N et al. Beclin1-driven autophagy modulates the inflammatory response of microglia via NLRP3. EMBO J. 2019-01-07 [PMID: 30617086] (WB, Mouse)
• Ugun-klusek A, Theodosi TS, Fitzgerald JC et al. Monoamine oxidase-A promotes protective autophagy in human SH-SY5Y neuroblastoma cells through Bcl-2 phosphorylation. Redox Biol. 2018-10-09 [PMID: 30336354] (WB, Human)
• Zhu Y, Fang J, Wang H et al. Baicalin suppresses proliferation, migration, and invasion in human glioblastoma cells via Ca2+-dependent pathway Drug Des Devel Ther 2018-10-02 [PMID: 30323558] (WB, Human)
• Sacitharan PK, Lwin S, Gharios GB, Edwards JR. Spermidine restores dysregulated autophagy and polyamine synthesis in aged and osteoarthritic chondrocytes via EP300. Exp. Mol. Med. 2018-09-19 [PMID: 30232322] (WB, Human, Mouse)
• Dutta K, Patel P, Julien JP. Protective effects of Withania somnifera extract in SOD1G93A mouse model of amyotrophic lateral sclerosis Exp. Neurol. 2018-08-19 [PMID: 30134145] (WB, Mouse)
• Li , Wang C, Li Z et al. Nano-sized Al2O3 particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts Cell Death Dis 2018-08-12 [PMID: 30082761] (IHC, WB, Human)
• Ognibene M, Pagnan G, Marimpietri D et al. CHL1 gene acts as a tumor suppressor in human neuroblastoma Oncotarget. [PMID: 29899830] (WB, Human)
• Lapierre J, Rodriguez M, Ojha CR, El-Hage N Critical Role of Beclin1 in HIV Tat and Morphine-Induced Inflammation and Calcium Release in Glial Cells from Autophagy Deficient Mouse. J Neuroimmune Pharmacol. 2018-05-11 [PMID: 29752681] (Mouse)
  
  Details:
  Mice were infected with S. pneumoniae.
• Kivinen N. The role of autophagy in age-related macular degeneration. Acta Ophthalmol 2018-04-01 [PMID: 29633521] (IHC-P, Mouse)
• Li YL, Cai WF, Wang L et al. Identification of the Functional Autophagy-Regulatory Domain in HCLS1-Associated Protein X-1 That Resists Against Oxidative Stress. DNA Cell Biol 2018-05-01 [PMID: 29461873] (Human)
• Lim Ji Hee, Kim Hyung Wook, Kim Min Young et al. Cinacalcet-mediated activation of the CaMKK beta-LKB1-AMPK pathway attenuates diabetic nephropathy in db/db mice by modulation of apoptosis and autophagy. Bmc Cancer 2018-02-15 [PMID: 29449563] (WB, Mouse)
• Kim YH, Kwak MS, Shin JM et al. Inflachromene inhibits autophagy through modulation of Beclin 1 activity. J Cell Sci 2018-02-20 [PMID: 29361549]
• Guo XL, Hu F, Wang H et al. Inhibition of autophagy in hepatocarcinoma cells promotes chemotherapeutic agent-induced apoptosis during nutrient deprivation Oncol Rep. 2018-02-01 [PMID: 29207161] (WB)
  
  Details:
  Citation using the HRP form of this antibody.
• Liu GS, Zhu H, Cai WF et al. Regulation of BECN1-Mediated Autophagy by HSPB6: Insights from A Human HSPB6S10F Mutant Autophagy. 2017-11-20 [PMID: 29157081] (Human)
• Chang RK, Li X, Mu N et al. MicroRNA expression profiles in non-epithelial ovarian tumors. Int. J. Oncol. 2017-11-10 [PMID: 29138809] (WB, Human)
• Ouyang YB, Ning S, Adler JR et al. Alteration of Interneuron Immunoreactivity and Autophagic Activity in Rat Hippocampus after Single High-Dose Whole-Brain Irradiation. Cureus 2017-06-30 [PMID: 28861331] (ICC/IF, Rat)
• Li Q, Han Y, Du J et al. Alterations of apoptosis and autophagy in developing brain of rats with epilepsy: Changes in LC3, P62, Beclin-1 and Bcl-2 levels Neurosci. Res. 2017-08-12 [PMID: 28807642] (WB, Rat)
• Wang CY, Hong YH, Syu JS et al. LRWD1 Regulates Microtubule Nucleation and Proper Cell Cycle Progression in the Human Testicular Embryonic Carcinoma Cells. J. Cell. Biochem. 2017-06-01 [PMID: 28569402]
• Gao Y, Zhuang Z, Gao S et al. Tetrahydrocurcumin reduces oxidative stress-induced apoptosis via the mitochondrial apoptotic pathway by modulating autophagy in rats after traumatic brain injury. Am J Transl Res 2017-03-15 [PMID: 28386319] (WB, Rat)
• Gelmetti V, DeRosa P, Torosantucci L et al. PINK1 and BECN1 relocalize at mitochondria-associated membranes during mitophagy and promote ER-mitochondria tethering and autophagosome formation. Autophagy. 2016-12-23 [PMID: 28368777] (ICC/IF, IP, WB, Human)
• Huang W, Ao P, Li J et al. Autophagy protects advanced glycation end product-induced apoptosis and. BioMed Research International. 2017-01-16 [PMID: 28265573] (WB, Rat)
• Eritja N, Chen BJ, Rodriguez-Barrueco R et al. Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer Autophagy 2017-01-05 [PMID: 28055301] (WB, Human)
• Ugun-Klusek A, Tatham MH, Elkharaz J et al. Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway Cell Death Dis 2017-01-05 [PMID: 28055010] (IHC, Mouse)
• Yang Y, Guo C, Liao B et al. BAMBI inhibits inflammation through the activation of autophagy in experimental spinal cord injury Int. J. Mol. Med 2017-02-01 [PMID: 28035406] (Rat)
• Bortnik S, Choutka C, Horlings HM et al. Identification of breast cancer cell subtypes sensitive to ATG4B inhibition. Oncotarget. 2016-10-11 [PMID: 27556700] (WB, Human)
• Personal communications with Dr Beth Levine, Columbia University.
• Rodriguez Myosotys, Lapierre Jessica, Ojha Chet Raj et al. Intranasal drug delivery of small interfering RNA targeting Beclin1 encapsulated with polyethylenimine (PEI) in mouse brain to achieve HIV attenuation. Sci Rep 2017-01-01 [PMID: 28500326] (WB, Mouse)
• Zhang L, Wang H, Fan Y et al. Fucoxanthin provides neuroprotection in models of traumatic brain injury via the Nrf2-ARE and Nrf2-autophagy pathways Sci Rep. 2017-04-20 [PMID: 28429775] (WB, Mouse)
• Syu J. S, Baba T, et al. Lysosomal activity maintains glycolysis and cyclin E1 expression by mediating Ad4BP/SF-1 stability for proper steroidogenic cell growth. Sci Rep 2017-03-21 [PMID: 28325912] (WB, Mouse)
• Jang JE, Eom JI, Jeung HK et al. AMPK-ULK1-mediated autophagy confers resistance to BET inhibitor JQ1 in acute myeloid leukemia stem cells. Clin. Cancer Res. 2016-11-18 [PMID: 27864418] (WB, Human)
• Hu YB, Zou Y, Huang Y et al. ROCK1 Is Associated with Alzheimer's Disease-Specific Plaques, as well as Enhances Autophagosome Formation But not Autophagic Abeta Clearance. Front Cell Neurosci. 2016-11-17 [PMID: 27853422] (IHC, Human)
• Weh KM, Howell AB, Kresty LA et al. Expression, Modulation, and Clinical Correlates of the Autophagy Protein Beclin-1 in Esophageal Adenocarcinoma Mol Carcinog. 2015-11-18 [PMID: 27696537] (IHC-P, Human)
• Hong JM, Kim SJ, Lee SM. Role of necroptosis in autophagy signaling during hepatic ischemia and reperfusion. Toxicol. Appl. Pharmacol. 2016-08-10 [PMID: 27521978] (WB)
• Shen P, Chen M, He M et al. Inhibition of ERa/ERK/P62 cascades induces autophagic switch in the estrogen receptor-positive breast cancer cells exposed to gemcitabine Oncotarget 2016-07-26 [PMID: 27384485] (WB, Human)
• Kivinen N, Felszeghy S, Kinnunen AI et al. Absence of collagen XVIII in mice causes age-related insufficiency in retinal pigment epithelium proteostasis. Biogerontology. 2016-04-28 [PMID: 27125427] (IHC-P, Mouse)
• Choudhury KR, Bucha S, Baksi S et al. Chaperone-like protein HYPK and its interacting partners augment autophagy. Eur. J. Cell Biol. 2016-04-01 [PMID: 27067261] (WB, Mouse)
• Ueno T, Saji S, Sugimoto M et al. Clinical significance of the expression of autophagy-associated marker, beclin 1, in breast cancer patients who received neoadjuvant endocrine therapy. BMC Cancer. 2016-03-17 [PMID: 26984766] (IHC-P, Human)
• Cheong JW, Kim Y, Eom JI et al. Enhanced autophagy in cytarabine arabinoside-resistant U937 leukemia cells and its potential as a target for overcoming resistance. Mol Med Rep 2016-04-01 [PMID: 26935591] (WB)
• Wang HS, Pei F, Chen Z, Zhang L Increased Apoptosis of Inflamed Odontoblasts Is Associated with CD47 Loss. J Dent Res 2016-06-01 [PMID: 26912219]
• Tsao Yu-Tzu, Huang Yu-Fen, Kuo Chun-Yu et al. Hinokitiol Inhibits Melanogenesis via AKT/mTOR Signaling in B16F10 Mouse Melanoma Cells. Int J Mol Sci 2016-01-01 [PMID: 26901194] (WB, Mouse)
• Masuda GO, Yashiro M, Kitayama K et al. Clinicopathological Correlations of Autophagy-related Proteins LC3, Beclin 1 and p62 in Gastric Cancer. Anticancer Res. 2016-01-01 [PMID: 26722036] (IHC-P, Human)
• Park YS, Park JH, Ko J et al. mTOR inhibition by rapamycin protects against deltamethrin-induced apoptosis in PC12 Cells. Environ. Toxicol. 2015-11-21 [PMID: 26588882] (WB, Rat)
• Majumder P, Chakrabarti O. Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101. Cell Death Dis. 2015-11-06 [PMID: 26539917] (WB, Human)
• Liu L, Li CJ, Lu Y et al. Baclofen mediates neuroprotection on hippocampal CA1 pyramidal cells through the regulation of autophagy under chronic cerebral hypoperfusion. Sci Rep 2015-01-01 [PMID: 26412641] (WB)
• Tanji K, Miki Y, Maruyama A et al. Trehalose intake induces chaperone molecules along with autophagy in a mouse model of Lewy body disease. Biochem. Biophys. Res. Commun. 2015-08-20 [PMID: 26299928] (WB, Mouse)
• Siddiqui MA, Mukherjee S, Manivannan P, Malathi K. RNase L Cleavage Products Promote Switch from Autophagy to Apoptosis by Caspase-Mediated Cleavage of Beclin-1. Int J Mol Sci. 2015-08-12 [PMID: 26263979] (WB, Human)
  
  Details:
  Beclin 1/ATG6 antibody was used for WB assays in experiments involving human fibrosarcoma cell line HT1080. WB was also performed on cells that were transfected with 20nM of Beclin 1 siRNAs or control siRNAs.
• Dever SM, Rodriguez M, Lapierre J et al. Differing roles of autophagy in HIV-associated neurocognitive impairment and encephalitis with implications for morphine co-exposure. Front Microbiol 2015-01-01 [PMID: 26217309] (WB)
• Zhang L, Ding K, Wang H et al. Traumatic Brain Injury-Induced Neuronal Apoptosis is Reduced Through Modulation of PI3K and Autophagy Pathways in Mouse by FTY720 Cell. Mol. Neurobiol. 2015-06-23 [PMID: 26099903] (WB, Mouse)
• Zhang R, Li Y, Wang Z et al. Interference with HMGB1 increases the sensitivity to chemotherapy drugs by inhibiting HMGB1-mediated cell autophagy and inducing cell apoptosis. Tumour Biol 2015-06-04 [PMID: 26040768]
• Kaushik S, Cuervo AM Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis Nat Cell Biol. 2015-05-10 [PMID: 25961502] (ICC/IF, WB, Mouse, Rat)
• Zhang L, Wang H, Ding K, Xu J. FTY720 induces autophagy-related apoptosis and necroptosis in human glioblastoma cells. Toxicol. Lett. 2015-07-02 [PMID: 25939952] (WB, Human)
• Ariadna Laguna, Nicoletta Schintu, Andre Nobre et al. Dopaminergic control of autophagic-lysosomal function implicates Lmx1b in Parkinsons disease. Nature Neuroscience 2015-04-27 [PMID: 25915474] (WB, Mouse)
• Casalino-Matsuda SM, Nair A, Beitel GJ et al. Hypercapnia Inhibits Autophagy and Bacterial Killing in Human Macrophages by Increasing Expression of Bcl-2 and Bcl-xL J. Immunol 2015-04-20 [PMID: 25895534] (WB, Human)
  
  Details:
  Using the HRP conjugated version of NB500-249, catalog number NB500-249H.
• Wakabayashi K, Mori F, Kakita A et al. Analysis of microRNA from archived formalin-fixed paraffin-embedded specimens of amyotrophic lateral sclerosis. Acta Neuropathol Commun. 2014-12-14 [PMID: 25497327] (IHC-P, Human)
• Chen M, He M et al. The cytoprotective role of gemcitabine-induced autophagy associated with apoptosis inhibition in triple-negative MDA-MB-231 breast cancer cells. Int J Mol Med 2014-01-07 [PMID: 24804820] (WB, Human)
• Lim SW, Doh KC, Jin L et al. Ginseng Treatment Attenuates Autophagic Cell Death in Chronic Cyclosporine Nephropathy. Nephrology (Carlton). 2014-05-05 [PMID: 24796922] (WB, Mouse)
• Stellrecht CM, Vangapandu HV, Le XF et al. ATP directed agent, 8-chloro-adenosine, induces AMP activated protein kinase activity, leading to autophagic cell death in breast cancer cells. J Hematol Oncol 2014-03-21 [PMID: 24628795] (WB, Human)
• Dai DF, Karunadharma PP, Chiao YA et al. Altered proteome turnover and remodeling by short-term caloric restriction or rapamycin rejuvenate the aging heart. Aging Cell 2014-02-25 [PMID: 24612461] (WB, Mouse)
• Murphy Karen E, Gysbers Amanda M, Abbott Sarah K et al. Reduced glucocerebrosidase is associated with increased alpha-synuclein in sporadic Parkinson's disease. Brain. 2014-01-28 [PMID: 24477431] (WB, Human)
• Lee CH, Lin ST, Liu JJ et al. Salmonella induce autophagy in melanoma by the downregulation of AKT/mTOR pathway. Gene Ther. 2014-03-06 [PMID: 24451116] (WB, Mouse)
• Choi Soyoung, Shin Hyejin, Song Haengseok, Lim Hyunjung Jade. Suppression of autophagic activation in the mouse uterus by estrogen and progesterone. J Endocrinol. 2014-03-07 [PMID: 24443716] (Mouse)
• Romao S, Gasser N, Becker AC et al. Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing. J Cell Biol 2013-12-09 [PMID: 24322427] (ICC/IF, Human)
• Joshi-Barr S, Bett C, Chiang WC et al. De novo prion aggregates trigger autophagy in skeletal muscle. J Virol 2013-12-04 [PMID: 24307586] (WB, Mouse)
• Shin JN, Abdel Fattah E, Bhattacharya A et al. Inflammasome activation by altered proteostasis. J Biol Chem. 2013-10-31 [PMID: 24178293] (WB, Mouse)
• Roy K, Raychaudhuri M, Chakrabarti O, Mukhopadhyay D. Growth Factor Receptor-Bound Protein 2 Promotes Autophagic Removal of Amyloid-beta Protein Precursor Intracellular Domain Overload in Neuronal Cells. J Alzheimers Dis. 2013-10-07 [PMID: 24100123]
• Pampliega O, Orhon I, Patel B et al. Functional interaction between autophagy and ciliogenesis. Nature. 2013-10-10 [PMID: 24089209] (ICC/IF, Mouse)
• Casadei N, Pohler AM, Tomas-Zapico C et al. Overexpression of synphilin-1 promotes clearance of soluble and misfolded alpha-synuclein without restoring the motor phenotype in aged A30P transgenic mice. Hum. Mol. Genet. 2014-02-01 [PMID: 24064336] (WB, Mouse)
• Song YJ, Zhang SS, Guo XL et al. Autophagy contributes to the survival of CD133+ liver cancer stem cells in the hypoxic and nutrient-deprived tumor microenvironment. Cancer Lett 2013-07-20 [PMID: 23879969] (WB, Human)
  
  Details:
  Using the HRP conjugated version of NB500-249, catalog number NB500-249H.
• O'Seaghdha M, Wessels MR. Streptolysin O and its Co-Toxin NAD-glycohydrolase Protect Group A Streptococcus from Xenophagic Killing. PLoS Pathog 2013-06-01 [PMID: 23762025] (WB, Human)
• Yang M, Cao L, Xie M et al. Chloroquine Inhibits HMGB1 Inflammatory Signaling and Protects Mice from Lethal Sepsis. Biochem Pharmacol 2013-05-22 [PMID: 23707973] (WB, Mouse)
• Decressac M, Mattsson B, Weikop P et al. TFEB-mediated autophagy rescues midbrain dopamine neurons from alpha-synuclein toxicity. Proc Natl Acad Sci U S A 2013-04-22 [PMID: 23610405] (ICC/IF, IHC, WB, Rat)
• Ding Z, Liu S, Wang X et al. Oxidant stress in mitochondrial DNA damage, autophagy and inflammation in atherosclerosis. Sci Rep 2013-01-01 [PMID: 23326634] (WB, Human)
• Ding Z, Wang X, Schnackenberg L et al. Regulation of autophagy and apoptosis in response to ox-LDL in vascular smooth muscle cells, and the modulatory effects of the microRNA hsa-let-7g Int J Cardiol 2013-01-07 [PMID: 23305858] (ICC/IF, IHC, Mouse)
• Zhu S, Cao L, Yu Y et al. Inhibiting autophagy potentiates the anticancer activity of IFN1@/ IFNalpha in chronic myeloid leukemia cells. Autophagy 2012-12-14 [PMID: 23242206] (WB, Human)
• Wong E, Bejarano E, Rakshit M et al. Molecular determinants of selective clearance of protein inclusions by autophagy. Nat Commun. 2012-12-04 [PMID: 23212369] (ICC/IF, Human)
• Siddiqui MA, Malathi K. RNase L induces autophagy via c-Jun N-terminal Kinase and Double-Stranded RNA-Dependent Protein Kinase Signaling Pathways J Biol Chem 2012-10-29 [PMID: 23109342] (IP, Human)
• Geng QR, Xu DZ, He LJ et al. Beclin-1 Expression Is a Significant Predictor of Survival in Patients with Lymph Node-Positive Gastric Cancer PLoS One 2012-01-01 [PMID: 23029344] (IHC, WB, Human)
• Fougeray S, Mami I, Bertho G, Beaune P et al. Tryptophan Depletion and the Kinase GCN2 Mediate IFN--Induced Autophagy J Immunol 2012-09-15 [PMID: 22896630] (WB, Human)
• Lim SW, Hyoung BJ, Piao SG et al. Chronic cyclosporine nephropathy is characterized by excessive autophagosome formation and decreased autophagic clearance Transplantation 2012-08-15 [PMID: 22790389] (WB, Mouse)
• Dehay B, Ramirez A, Martinez-Vicente M, Perier C, Canron MH, Doudnikoff E, Vital A, Vila M, Klein C, Bezard E. Loss of P-type ATPase ATP13A2/PARK9 function induces general lysosomal deficiency and leads to Parkinson disease neurodegeneration. Proc Natl Acad Sci U S A. 2012-05-30 [PMID: 22647602] (WB, Human)
• Oliver L, Hue E, Priault M, Vallette FM. Basal Autophagy Decreased During the Differentiation of Human Adult Mesenchymal Stem Cells Stem Cells Dev 2012-06-13 [PMID: 22519885]
• Yu Y, Yang L, Zhao M et al. Targeting microRNA-30a-mediated autophagy enhances imatinib activity against human chronic myeloid leukemia cells Leukemia 2012-03-07 [PMID: 22395361] (WB, Human)
• Ceteci F, Xu J, Ceteci S et al. Conditional expression of oncogenic C-RAF in mouse pulmonary epithelial cells reveals differential tumorigenesis and induction of autophagy leading to tumor regression. Neoplasia 2011-11-01 [PMID: 22131876] (WB)
• Dash R, Azab B, Quinn B et al. Apogossypol derivative BI-97C1 (Sabutoclax) targeting Mcl-1 sensitizes prostate cancer cells to mda-7/IL-24-mediated toxicity Proc Natl Acad Sci U S A 2011-05-24 [PMID: 21555592] (IP, WB, Human)
• Elgendy M, Sheridan C, Brumatti G et al. Oncogenic Ras-induced expression of Noxa and Beclin-1 promotes autophagic cell death and limits clonogenic survival. Mol Cell. 2011-04-01 [PMID: 21353614] (WB, Human)
• Cheng HC, Kim SR, Oo TF et al. Akt Suppresses Retrograde Degeneration of Dopaminergic Axons by Inhibition of Macroautophagy. J Neurosci 2011-02-09 [PMID: 21307249]
• Belibi F, Zafar I, Ravichandran K et al. Hypoxia-inducible factor 1{alpha} (HIF-1 {alpha}) and autophagy in polycystic kidney disease (PKD). Am J Physiol Renal Physiol. 2011-01-01 [PMID: 21270095] (WB, Mouse, Rat)
• Monaco-Shawver L, Schwartz L, Tuluc F et al. Substance P inhibits natural killer cell cytotoxicity through the neurokinin-1 receptor. J Leukoc Biol;89(1):113-25. 2011-01-01 [PMID: 20940324]
• Zhang, N, Qi, Y Et al. FTY720 induces necrotic cell death and autophagy in ovarian cancer cells: a protective role of autophagy. Autophagy 2010-11-01 [PMID: 20935520] (IHC, Bovine)
• Song J, Qu Z, Guo X et al. Hypoxia-induced autophagy contributes to the chemoresistance of hepatocellular carcinoma cells. Autophagy 2009-11-01 [PMID: 19786832] (WB, Human)
  
  Details:
  Using the HRP conjugated version of NB500-249, catalog number NB500-249H.
• Pattingre S, Bauvy C, Carpentier S et al. Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy. J Biol Chem;284(5):2719-28. 2009-01-30 [PMID: 19029119] (WB, Human)
• Gulati P, Gaspers LD, Dann SG et al. Amino acids activate mTOR complex 1 via Ca2+/CaM signaling to hVps34. Cell Metab;7(5):456-65. 2008-05-01 [PMID: 18460336] (WB, Human)
• Zhang H, Bosch-Marce M, Shimoda LA et al. Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia. J Biol Chem;283(16):10892-903. 2008-04-18 [PMID: 18281291] (WB, Mouse)
• Massey, A C, Follenzi, A, Kiffin, R et al. Early cellular changes after blockage of chaperone-mediated autophagy. Autophagy; 4:4, 442-456. 2008-05-16 [PMID: 18253088] (WB, Mouse)
• Wang, Y, R Singh et al. Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus. J. Biol. Chem. 283: 4766-4777. 2008-01-01 [PMID: 18073215] (WB, Mouse)
• Park,K et al. A Human scFv Antibody against TRAIL Receptor 2 Induces Autophagic Cell Death in Both TRAIL-Sensitive TRAIL-Resistant Cancer Cells. Cancer Res; 67: 7327 - 7334. [PMID: 17671202] (IP, WB, Human)

Reviews for Beclin 1 Antibody (NB500-249) (9)

Average Rating: 3.9

(Based on 9 reviews)

We have 9 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Lucia Lisi WB Rat 05/20/2021

Summary

• Application: Western Blot
• Sample Tested: Microglia
• Species: Rat
• Lot: v-3

reviewed by: Jee-Eun Jung IHC-Fr Rat 02/21/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain tissue
• Species: Rat
• Lot: U-2

reviewed by: Milos Mandic WB Human 12/11/2017

Summary

• Application: Western Blot
• Sample Tested: Brain tissue
• Species: Human
• Lot: U-2

reviewed by: Liyong Zhang WB Mouse 12/08/2017

Summary

• Application: Western Blot
• Sample Tested: Primary mouse hepatocytes
• Species: Mouse
• Lot: U-2

Comments

• Comments: mouse hepatocytes were treated with 100 ng/ml LPS for 4 hours

reviewed by: Wenzhou Huang WB Rat 01/18/2017

Summary

• Application: Western Blot
• Sample Tested: Chondrocyte cell lysate
• Species: Rat

reviewed by: Verified Customer IHC-P Human 03/05/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human ovarian carcinoma -patient derived xenograft
• Species: Human
• Lot: K-2

reviewed by: Kelly Hudkins IHC-P Mouse 10/08/2012

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse kidney
• Species: Mouse
• Lot: J

reviewed by: Verified Customer WB Mouse 10/06/2012

Summary

• Application: Western Blot
• Sample Tested: Mouse whole cell lysate
• Species: Mouse

reviewed by: Amy Exum IHC Human 09/28/2010

Summary

• Application: Immunohistochemistry
• Sample Tested: Human Esophagus tissue
• Species: Human

FAQs for Beclin 1 Antibody (NB500-249). (Showing 1 - 9 of 9 FAQs).

1. I am interested in Novus' Beclin 1 peptide NB500-249PEP. Would you please send me the sequence information, and any data you may have for this peptide?
   • In regards to your inquiry, this Beclin 1 peptide is useful as a blocking peptide to our primary antibody NB500-249. This peptide contains amino acids that fall within the range of 1-100. Unfortunately the exact sequence is deemed proprietary by the lab.
2. I'm looking for a Beclin 1 antibody, which do you recommend for use in western blot?
   • Our best selling beclin 1 antibody NB500-249, has been cited in 41 publications where it was used in western blot. Additionally this beclin 1 antibody is typically available for immediate shipment.
3. I've had good results with your beclin antibody in IHC but now I need a beclin one antibody to use in ELISA, will this beclin antibody also work as a capture antibody in ELISA?
   • This particular beclin 1 antibody has not yet been validated for use in ELISA, we do however have 2 beclin 1 antibodies that are validated in ELISA, NBP2-22176 and NBP1-76648. Alternatively you could take advantage of our innovator's reward program which would allow you to test this beclin 1 antibody in ELISA with out financial risk. Just purchase the beclin antibody and test in ELISA, share your results (negative or positive) and we will issue a voucher for the purchase price of the antibody.
4. Is your belcin 1 antibody NB500-249 available as an atto 488 direct conjugate?
   • Unfortunately we do not offer Atto conjugates but we do offer this beclin 1 antibody as an AlexaFlour 488 direct conjugate and as a DyLight 488 conjugated antibody.
5. Can a Lightning-Link HRP labeling kit be used to label the beclin 1 antibody NB500-249, I've used it to label mouse isotypes but wasn't sure if it will work on a rabbit IgG antibody like this beclin antibody?
   • I can't find any specific mention of this beclin 1 antibody being labeled with a lightning link kit; however lightning link kits are extreamly versatile in terms of labeling ability and should be able to label this beclin 1 antibody without any trouble.
6. I'm using the ATDC5 murine carcinoma cell line, has beclin 1 antibody been documented as working in this cell line?
   • From a cursory look through our publication list I don't see that this beclin 1 antibody has been published as being used in that cell line. We show this beclin 1 antibody was published 19 times as being used in mouse, if I can get your contact info I will take a more in depth look to see if the beclin 1 antibody has been used in that cell line.
7. I'm doning an autophagy study using CHO-K1 cells, do you know if this beclin 1 antibody will detect beclin in that cell line?
   • Based on tissue expression data from protein atlas beclin 1 is not highly expressed in ovarian tissue; however in ovarian cancer tissues beclin 1 expression is much greater. We don't have any beclin 1 antibodies that have been validated for use in hamster so you may want to take advantage of our innovator's reward program.
8. The beclin 1 antibody NB500-249 has good data but I need a beclin atibody that will work with bovine tissue but I don't see bovine listed as reactive with the beclin 1 antibody. Is bovine not listed because you know this beclin one antibody will not recognize?
   • Although reactivity with bovine has not been experimentally validated, a comparison of the human and bovine sequences within the immunogen range indicates that cross reactivity is likely. An alignment of the human beclin 1 sequence and bovine beclin 1 sequence shows there is 95% match in the region that was used as the immunogen.
9. Will this beclin 1 antibody detect beclin that is cleaved at the last 50 aa of the c-terminal?
   • Because the immunogen used to make this beclin 1 antibody falls within the first 100 amino acids of the N-terminal it should detect beclin 1 that is cleaved at the c-terminal.

Bioinformatics

• Gene Symbol: BECN1