Reactivity | Hu, Mu, Rt, Am, Ca, Fi, Pl, Ze, BvSpecies Glossary |
Applications | WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SB, IHC-WhMt |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Format | BSA Free |
Concentration | 1.0 mg/ml |
Immunogen | This LC3A Antibody was prepared from a synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492]. |
Localization | LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes. |
Marker | Autophagosome Marker |
Specificity | This LC3A Antibody detects both LC3A and LC3B. |
Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | MAP1LC3A |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In ICC, cytoplasmic staining was observed in HeLa cells. Use in immunocytochemistry (PMID: 21545732). and Immunohistochemistry-Paraffin (PMID: 26571030). reported in scientific literature. Use in Flow cytometry reported in scientific literature ( PMID: 24419333).. Use in ELISA reported in scientific literature (PMID: 20930550).. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 31783118).. Use in Southern blot reported in scientific literature (PMID: 21262964).. Use in immunoblotting reported in scientific literature (PMID: 28253371).. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:33035707). |
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Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Lucia Lisi |
Immunocytochemistry | Human | 05/21/2021 |
Summary
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reviewed by:
Lucia Lisi |
Western Blot | Rat | 05/21/2021 |
Summary
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reviewed by:
Eric Wang |
Western Blot | Human | 06/09/2020 |
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reviewed by:
Marie Lassont |
Western Blot | Mouse | 01/29/2019 |
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reviewed by:
Liyong Zhang |
Western Blot | Human and Mouse | 11/25/2017 |
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reviewed by:
Du Jiuju |
Western Blot | Mouse | 08/07/2017 |
Summary
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reviewed by:
Verified Customer |
Western Blot | Mouse | 10/06/2015 |
Summary
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reviewed by:
Ilya Ulasov |
Western Blot | Human | 01/12/2015 |
Summary
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reviewed by:
cynthia morimoto |
Western Blot | Mouse | 12/12/2014 |
Summary
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reviewed by:
Bindi Patel |
Immunofluorescence | Mouse | 11/25/2014 |
Summary
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reviewed by:
Verified Customer |
Immunohistochemistry-Paraffin | 10/04/2014 |
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reviewed by:
Verified Customer |
Western Blot | Human | 06/30/2014 |
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reviewed by:
Fabio Di Domenico |
Western Blot | Human | 06/20/2014 |
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reviewed by:
Verified Customer |
Western Blot | Human | 04/02/2014 |
Summary
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reviewed by:
Verified Customer |
Immunohistochemistry | Mouse | 08/13/2012 |
Summary
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reviewed by:
Jay Kao |
Western Blot | Human | 12/28/2011 |
Summary
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reviewed by:
Ming-Tsang Chiao |
Western Blot | Human | 11/21/2011 |
Summary
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reviewed by:
Ming-Shiou Jan |
Western Blot | Human | 11/21/2011 |
Summary
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reviewed by:
Che-Hsu Lee |
Western Blot | Mouse | 10/24/2011 |
Summary
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reviewed by:
Verified Customer |
Western Blot | Mouse | 07/08/2010 |
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reviewed by:
System Admin |
Western Blot | Other | 11/05/2009 |
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reviewed by:
System Admin |
Western Blot | Human | 02/18/2009 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for LC3A Antibody (NB100-2331)Discover more about diseases related to LC3A Antibody (NB100-2331).
| Pathways for LC3A Antibody (NB100-2331)View related products by pathway.
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PTMs for LC3A Antibody (NB100-2331)Learn more about PTMs related to LC3A Antibody (NB100-2331).
| Research Areas for LC3A Antibody (NB100-2331)Find related products by research area.
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The LC3 A, B, C’s and 1, 2, 3’s By Christina Towers, PhD Autophagy is a catabolic process used to breakdown and recycle damaged proteins and organelles. It is a multistep process that, in its simplest form, consists of 4 steps: initiation, phago... Read full blog post. |
Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease By Jamshed Arslan Pharm.D. Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy... Read full blog post. |
Nuclear LC3: Why is it there and what is it doing? By Christina Towers, PhD. Cells use the complex process of autophagy to degrade and recycle cytoplasmic material. There are over 20 proteins that have been implicated in this process and appropriately named core ... Read full blog post. |
Why LC3B Antibodies Make Ideal Autophagosomes Membrane Markers The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants LC3A, LC3B, and LC3C.1 LC3B is a subunit of the MAP1A and MAP1B microtubule-binding proteins and plays a central role in autophagosome membrane stru... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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Lucia Lisi 05/21/2021 |
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Application: | Immunocytochemistry | |
Species: | Human |
Lucia Lisi 05/21/2021 |
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Application: | Western Blot | |
Species: | Rat |
Eric Wang 06/09/2020 |
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Application: | Western Blot | |
Species: | Human |
Gene Symbol | MAP1LC3A |