ATG4B Antibody

Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.

Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-24709] - Analysis of human heart using NBP2-24709 at 10 ug/ml.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm, Ma-Op, Pm-Or
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

ATG4B Antibody Summary

• Immunogen: A portion of amino acid 350-400 of human APG4B was used as the immunogen.
• Predicted Species: Monkey (100%), Canine (100%), Opossum (94%), Chimpanzee (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG4B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-3 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 88% in Zebrafish and Porcine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for ATG4B Antibody

• APG4 autophagy 4 homolog B (S. cerevisiae)
• APG4 autophagy 4 homolog B
• Apg4B
• ATG4 autophagy related 4 homolog B (S. cerevisiae)
• ATG4B
• AUTL1
• AUTL1MGC1353
• AUT-like 1 cysteine endopeptidase
• autophagin-1
• Autophagy-related cysteine endopeptidase 1
• Autophagy-related protein 4 homolog B
• cysteine protease ATG4B
• DKFZp586D1822
• EC 3.4.22
• EC 3.4.22.-
• hAPG4B
• KIAA0943

Background

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG4B