ATG4B Antibody

Images

 
Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.
Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-24709] - Analysis of human heart using NBP2-24709 at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, Pm, Ma-Op, Pm-OrSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

ATG4B Antibody Summary

Immunogen
A portion of amino acid 350-400 of human APG4B was used as the immunogen.
Predicted Species
Monkey (100%), Canine (100%), Opossum (94%), Chimpanzee (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG4B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-3 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 88% in Zebrafish and Porcine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for ATG4B Antibody

  • APG4 autophagy 4 homolog B (S. cerevisiae)
  • APG4 autophagy 4 homolog B
  • Apg4B
  • ATG4 autophagy related 4 homolog B (S. cerevisiae)
  • ATG4B
  • AUTL1
  • AUTL1MGC1353
  • AUT-like 1 cysteine endopeptidase
  • autophagin-1
  • Autophagy-related cysteine endopeptidase 1
  • Autophagy-related protein 4 homolog B
  • cysteine protease ATG4B
  • DKFZp586D1822
  • EC 3.4.22
  • EC 3.4.22.-
  • hAPG4B
  • KIAA0943

Background

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Supplier Logo

Publications for ATG4B Antibody (NBP2-24709) (0)

There are no publications for ATG4B Antibody (NBP2-24709).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ATG4B Antibody (NBP2-24709) (0)

There are no reviews for ATG4B Antibody (NBP2-24709). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATG4B Antibody (NBP2-24709) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ATG4B Products

Research Areas for ATG4B Antibody (NBP2-24709)

Find related products by research area.

Blogs on ATG4B.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.


  Read full blog post.

ATG4B - a cysteine protease involved in autophagosome elongation
Autophagy can be broken down into 4 main stages: phagophore nucleation, autophagosome elongation, autophagosome docking and fusion with a lysosome, and vesicle breakdown and degradation. ATG4B is one of four ATG4 homologs (ATG4A, ATG4B, ATG4C, and ...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ATG4B Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ATG4B