Reactivity | Hu, Mu, Rt, Po, Al, Bv, Dr, Fi, Gp, Pm, Xp, ZeSpecies Glossary |
Applications | WB, Simple Western, ELISA, EM, Flow, IB, ICC/IF, IHC, IHC-P, IP, PLA, RIA, KD, KO |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Format | BSA Free |
Concentration | 1.0 mg/ml |
Immunogen | This ATG5 Antibody was made to a synthetic peptide of an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0]. |
Localization | Cytoplasm. Co-localizes with non-muscle actin. |
Specificity | This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing amino acids 1-79. The calpain cleaved form of ATG5 is missing amino acids 195-275. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | ATG5 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Theoretical MW | 32 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Control Peptide |
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Reviewed Applications |
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Publications |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Zeyuan Zhang |
WB | Mouse | 09/15/2020 |
Summary
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reviewed by:
Kyohei Nishino |
WB | Mouse | 03/31/2018 |
Summary
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reviewed by:
Liyong Zhang |
WB | Human | 12/15/2017 |
Summary
Comments
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reviewed by:
Verified Customer |
IHC-P | 10/04/2014 |
Summary
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reviewed by:
Verified Customer |
WB | Mouse | 09/21/2012 |
Summary
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reviewed by:
Lee Hsiao-Wei |
WB | Rat | 12/20/2011 |
Summary
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reviewed by:
Yu-Ting Yen |
WB | Mouse | 11/16/2011 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for ATG5 Antibody (NB110-53818)Discover more about diseases related to ATG5 Antibody (NB110-53818).
| Pathways for ATG5 Antibody (NB110-53818)View related products by pathway.
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PTMs for ATG5 Antibody (NB110-53818)Learn more about PTMs related to ATG5 Antibody (NB110-53818).
| Research Areas for ATG5 Antibody (NB110-53818)Find related products by research area.
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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit... Read full blog post. |
Autophagic flux: Is p62 a good indicator? By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to aut... Read full blog post. |
Best genes to knockout to control autophagic flux By Christina Towers, PhD Autophagy flux: Basic principlesAutophagic flux is defined as the amount of cellular material degraded and recycled through the process of autophagy, whereby cells break down and disca... Read full blog post. |
The LC3 A, B, C’s and 1, 2, 3’s By Christina Towers, PhD Autophagy is a catabolic process used to breakdown and recycle damaged proteins and organelles. It is a multistep process that, in its simplest form, consists of 4 steps: initiation, phago... Read full blog post. |
Animal Models to Study Autophagy By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the... Read full blog post. |
Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease By Jamshed Arslan Pharm.D. Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy... Read full blog post. |
Read full blog post. |
Autophagy independent roles of the core ATG proteins By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation. It is critical for removal of dam... Read full blog post. |
Key Targets in Apoptosis, Necroptosis, and Autophagy Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and ... Read full blog post. |
The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function. The two key players of this pathway are PINK1, ... Read full blog post. |
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Zeyuan Zhang 09/15/2020 |
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Application: | WB | |
Species: | Mouse |
Kyohei Nishino 03/31/2018 |
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Application: | WB | |
Species: | Mouse |
Liyong Zhang 12/15/2017 |
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Application: | WB | |
Species: | Human |
Gene Symbol | ATG5 |