ATG5 Antibody - BSA Free

Images

 
Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for ...read more
CQ increased CDDP-induced cell death in drug-resistant EOC through autophagy inhibition and induction of p21WAF1/CIP1.a A2780-CP20 cells stably expressing control or ATG5 shRNA were treated with CDDP for 48 h, and the ...read more
FIGURE 2: Blockage of autophagy leads to increased plp mRNA and protein levels. (B) Western blot analysis with antibodies against Atg5, P62, PLP and MBP proteins in optic nerve lysates of 6mo control and cKO mice. ...read more
LHPP suppresses aerobic glycolysis.A Relative mRNA expression levels of glycolytic genes and glutamine transporters in LHPP-overexpressing or LHPP-knockdown MGC-803 cells and control cells by qPCR. B Protein expression ...read more
WNT11 is induced by hypoxia or hypoxic mimetics in different cell types.(A) Increased Wnt11 mRNA in EMSC adipocytes (Day 12) after hypoxia-mimetic treatments. EMSC adipocytes were treated with CoCl2 (0.1 mM), DFO ...read more
Representative microscopy images of staining for hypoxia markers in prostate tissues (MO, 400×). A) HIF-1 alpha - notice the granular cytoplasmic immunoreactivity of the malignant epithelial cells. In this case, more ...read more
Interaction of Nesprin-2-SMC and Nesprin-2 with SMC2 and SMC4. (a) Precipitation of SMC2 and SMC4 with GST-Nesprin-2-SMC from HaCaT cell lysates. Precipitates were resolved on SDS-polyacrylamide gels (10% acrylamide) ...read more
Representative human colorectal cancer specimen. Immunohistochemistry of CA9 and GGT1 in mLNs. In these cases, both CA9 and GGT1 were expressed, suggesting that GGT1 expression is related to hypoxia in mLNs. Scale bar, ...read more
Modulation of A152T-tau clearance and pathology by upregulation of autophagy. (A and B) Treatment of A152T-tau fish with the autophagy inducers clonidine (A), rapamycin or rilmenidine (B), accelerated clearance kinetics ...read more
SAN1 functions independently of the FA pathway and does not affect FA pathway activation. a, b CSAs of HeLa WT and SAN1−/− cells treated with scrambled ctrl siRNA or FANCD2 siRNA, in response to Cisplatin and MMC ...read more
Activation of MAPK7 in chondrocytes is essential for hypoxic adaptation and enhancement of HIF1 alpha signaling under hypoxia. a, b Immunofluorescence staining of (a) MAPK7 (b) P-MAPK7 on representative sections of ...read more
Caspase-11 is expressed in Casp1Null bone marrow-derived macrophages.Immunoblot analysis of caspase-1, caspase-11 and GAPDH (loading control) in unprimed WT or mutant BMDMs at various times (above lane) after ...read more
ZNF217 and ALKBH5 regulate NANOG and KLF4 expression via modulation of m6A levelsA. MCF-7 subclones expressing a non-targeting control shRNA (NTC) or shRNAs targeting HIF-1 alpha and HIF-2 alpha (double knockdown ...read more
PML-RARa is a HIF-alpha transcriptional co-activator.A HIF-alpha transactivation assays (A–D) with HRE-luciferase construct. Results are presented as Luciferase/Renilla ratio (mean ± s.e.m. of experiments ...read more
Treatment of slice cultures from human renal tumors. Tissue slice cultures from human renal tumors were treated for 48 h with a panel of drugs (10 µM each) and cell viability assayed as in Figure 3A. (A) Intra-tumor ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Al, Bv, Dr, Fi, Gp, Pm, Xp, ZeSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IP, MS, WB, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

ATG5 Antibody - BSA Free Summary

Immunogen
This ATG5 Antibody was made to a synthetic peptide of an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0].
Localization
Cytoplasm. Co-localizes with non-muscle actin.
Specificity
This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing amino acids 1-79. The calpain cleaved form of ATG5 is missing amino acids 195-275.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy
  • ELISA
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Knockout Validated
  • Proximity Ligation Assay
  • Radioimmunoassay
  • Simple Western 1:50
  • Western Blot 1:500
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ATG5 Overexpression Lysate
Control Peptide
ATG5 Protein (NB110-53818PEP)
Reviewed Applications
Read 8 Reviews rated 4.3
using
NB110-53818 in the following applications:

Publications
Read Publications using
NB110-53818 in the following applications:

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID:33221768) Fish reactivity reported in scientific literature (PMID: 26183773). Guinea Pig reactivity reported in scientific literature (PMID: 30766882). Use in Alligator reported in scientific literature (PMID:32061056).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATG5 Antibody - BSA Free

  • APG5 (Autophagy 5, S. Cerevisiae)-Like
  • APG5 autophagy 5-like (S. cerevisiae)
  • APG5
  • APG5L
  • APG5-LIKE
  • ASP
  • ASPAPG5-LIKE
  • ATG5 autophagy related 5 homolog (S. cerevisiae)
  • ATG5 Autophagy Related 5 Homolog
  • ATG5
  • Autophagy protein 5
  • Autophagy Related 5
  • HAPG5
  • SCAR25

Background

Atg5, Autophagy related 5 or autophagy protein 5 (theoretical molecular weight 32 kDa), belongs to a group of core autophagy-related proteins first identified in yeast and later in eukaryotic cells. Atg proteins play essential roles in the process of macroautophagy. Atg5 is considered a core autophagy protein, for its role in the formation of the autophagosome, a double membrane vesicle which engulfs proteins and organelles for delivery to the lysosome and subsequent degradation (1). Atg5 participates in the process of phagophore elongation by interacting with the ubiquitin-like protein Atg12. Formation of the Atg12-Atg5 conjugate is dependent on the activities of Atg7 (E1 ubiquitin-activating like enzyme) and Atg10 (E2 ubiquitin-activating like enzyme). Non-covalent interaction between the Atg12-Atg5 conjugate and Atg16L1, allows for the formation of a large complex which associates with the nascent phagophore. The Atg16L1 complex dissociates from the autophagosome once it is fully formed (1,2).

In the context of its role in autophagy, Atg5 plays diverse physiologically relevant roles. For example, Atg5 together with Atg7 are required for adipogenesis (3). Recently, Atg5 has been implicated in the process of B-cell receptor polarization and antigen presentation (4). In addition to its role in autophagy, Atg5 is implicated in apoptotic cell death. Interaction of Atg5 with FADD (Fas-associated protein with death domain) is involved in cell death induced by IFN-gamma. A truncated form of Atg5, a 24kDa fragment, leads to cell death by interacting with Bcl-xl and inhibiting its anti-apoptotic activity (5). Other Atg5 interacting partners include interleukin-beta (IL-beta) converting enzyme and nucleotide binding oligomerization domain protein 1, which suggest that Atg5 may play other biologically relevant roles (3).

References

1. Yang, Z., & Klionsky, D. J. (2010). Mammalian autophagy: Core molecular machinery and signaling regulation. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2009.11.014

2. Rubinsztein, D. C., Shpilka, T., & Elazar, Z. (2012). Mechanisms of autophagosome biogenesis. Current Biology. https://doi.org/10.1016/j.cub.2011.11.034

3. Subramani, S., & Malhotra, V. (2013). Non-autophagic roles of autophagy-related proteins. EMBO Reports. https://doi.org/10.1038/embor.2012.220

4. Arbogast, F., Arnold, J., Hammann, P., Kuhn, L., Chicher, J., Murera, D., Gros, F. (2019). ATG5 is required for B cell polarization and presentation of particulate antigens. Autophagy. https://doi.org/10.1080/15548627.2018.1516327

5. Luo, S., & Rubinsztein, D. C. (2007). Atg5 and Bcl-2 provide novel insights into the interplay between apoptosis and autophagy. Cell Death and Differentiation. https://doi.org/10.1038/sj.cdd.4402149

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATG5 Antibody (NB110-53818)(265)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Alligator, Bovine, Drosophila, Drosophilia, Guinea Pig, Porcine, Primate, Zebrafish.

We have publications tested in 15 applications: B/N, ELISA, EM, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, In Vivo, KO, PLA, RIA, WB.


Filter By Application
B/N
(1)
ELISA
(1)
EM
(1)
FLOW
(1)
ICC/IF
(13)
IF/IHC
(12)
IHC
(1)
IHC-Fr
(1)
IHC-P
(9)
IP
(2)
In Vivo
(2)
KO
(1)
PLA
(1)
RIA
(1)
WB
(169)
All Applications
Filter By Species
Human
(71)
Mouse
(86)
Rat
(12)
Alligator
(1)
Bovine
(6)
Drosophila
(2)
Drosophilia
(1)
Guinea Pig
(1)
Porcine
(2)
Primate
(2)
Zebrafish
(10)
All Species
Showing Publications 1 - 10 of 265. Show All 265 Publications.
Publications using NB110-53818 Applications Species
Bai L, Zhang R, Zheng H et al. Seneca Valley Virus Degrades STING via PERK and ATF6-Mediated Reticulophagy Viruses 2023-11-03 [PMID: 38005886]
Mart�nez-Gonz�lez J, Fern�ndez-Carbonell �, Cant� A et al. Sequences of Alterations in Inflammation and Autophagy Processes in Rd1 Mice Biomolecules 2023-08-22 [PMID: 37759678] (WB, IHC) WB, IHC
Keary KM, Gu QH, Chen J, Li Z Dendritic distribution of autophagosomes underlies pathway-selective induction of LTD Cell reports 2023-07-28 [PMID: 37516958] (IHC-Fr, Mouse)

Details:
Dilutions: 1:500
IHC-Fr Mouse
Sakurai HT, Iwashita H, Arakawa S et al. Development of small fluorescent probes for the analysis of autophagy kinetics iScience 2023-07-21 [PMID: 37456828] (In Vivo) In Vivo
Pan Y, Yang Y, Fan M et al. Progranulin regulation of autophagy contributes to its chondroprotective effect in osteoarthritis Genes & Diseases 2023-07-01 [PMID: 37397563]
Amanda M, Artem P, Taylor D et al. Targeting the ATF6-mediated ER stress response and autophagy blocks integrin-driven prostate cancer progression Mol Cancer Res. 2023-06-14 [PMID: 37314749] (WB, Human) WB Human
Mitsui, K;Hishiyama, S;Jain, A;Kotoda, Y;Abe, M;Matsukawa, T;Kotoda, M; Role of macrophage autophagy in postoperative pain and inflammation in mice Journal of neuroinflammation 2023-05-02 [PMID: 37131209] (WB, Mouse) WB Mouse
Wan W, Qian C, Wang Q et al. STING directly recruits WIPI2 for autophagosome formation during STING-induced autophagy The EMBO journal 2023-03-06 [PMID: 36872914]
Liao YH, Wu JT, Hsieh IC et al. ARMS-NF-?B signaling regulates intracellular ROS to induce autophagy-associated cell death upon oxidative stress iScience 2023-02-17 [PMID: 36798436]
Lee HJ, Kim SH, Kim YH et al. Nalfurafine Hydrochloride, a ?-Opioid Receptor Agonist, Induces Melanophagy via PKA Inhibition in B16F1 Cells Cells 2022-12-29 [PMID: 36611940] (WB) WB
Show All 265 Publications.

Reviews for ATG5 Antibody (NB110-53818) (8) 4.38

Average Rating: 4.3
(Based on 8 reviews)
We have 8 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB110-53818:
Filter by Applications
WB
(7)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(4)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ATG5 NB110-53818
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5
reviewed by:
Armen Petrosyan
WB Human 06/27/2023
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Summary

ApplicationWestern Blot
Sample TestedDU145 human prostate carcinoma cell line
SpeciesHuman
LotP
Western Blot ATG5 NB110-53818
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4
reviewed by:
Verified Customer
WB Mouse 09/15/2020
View

Summary

ApplicationWestern Blot
Sample TestedLiver tissue
SpeciesMouse
LotI-1
  4
reviewed by:
Verified Customer
WB Mouse 03/31/2018
View

Summary

ApplicationWestern Blot
Sample TestedMouse Colon
SpeciesMouse
LotM-1
Western Blot ATG5 NB110-53818
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4
reviewed by:
Verified Customer
WB Human 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman parimary hepatocytes,Human primary hepatocytes
SpeciesHuman
LotK-3

Comments

Comments100 ng/ml LPS treated human primary hepatocytes
Immunohistochemistry-Paraffin ATG5 NB110-53818
Enlarge
4
reviewed by:
Merissa Olmer
IHC-P 10/04/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
LotE-3
Western Blot ATG5 NB110-53818
Enlarge
4
reviewed by:
Ann Marie Santiago
WB Mouse 09/21/2012
View

Summary

ApplicationWestern Blot
Sample TestedMurine hepatoma
SpeciesMouse
Lote-2
Western Blot ATG5 NB110-53818
Enlarge
4
reviewed by:
Verified Customer
WB Rat 12/20/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat Cardiomyocyte
SpeciesRat
  5
reviewed by:
Verified Customer
WB Mouse 11/16/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse

Product General Protocols

View specific protocols for ATG5 Antibody (NB110-53818): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATG5 Antibody (NB110-53818). (Showing 1 - 5 of 5 FAQs).

  1. I am interested in testing your anti ATG5 (NB110-53818SS) for Immunofluorescence in MDCK cell (canine epithelial cell line). I noticed you report reactivity of the antibody against several species but not dog. Do you know if the Ab will react against canine genome? Also for IF what fixation do you recommend?
    • The applications and species listed for any one of our antibodies are ones we have tested and validated. Canine is not listed as it has not been tested and therefore we cannot guarantee its cross reactivity. If you would be interested in testing this antibody in canine, we can recommend our Innovator's Reward. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. Learn more about our Innovators Reward Program. Also, please use this link to view our Immunofluorescence protocol which explains the fixation method that we recommend.
  2. Our customer is interested in this ATG5 antibody (cat. no. NB110-53818). Would you please confirm that this antibody would recognize ATG5 alone, as well as ATG5-ATG12 conjugate?
    • The antibody was raised against a peptide corresponding to the N-terminal of ATG5. It should recognize ATG5 alone but it appears that ATG5 is rarely found alone in the cell.
  3. The arrow in the data sheet points to the ATG5/ATG12 band of 56 kDa. There is also a band of 33 kDa which I guess is ATG5? Could you explain the meaning of detecting the ATG5/ATG12 conjugate in preference to ATG5?  Does the antibody cross-react with ATG12?
    • The Western blot image you are referring to illustrates the ATG5/ATG12 conjugate, which is formed during autophagy. During autophagy ATG5 is covalently bonds ATG12 in a ubiquitous reaction. The lysate that was used was meant to illustrate the ability to detect autophagy by Western blot via ATG5/ATG12. It is possible to detect free ATG5 in cell lysates, although it is not seen in this Western blot. The lower 2 bands do not correspond to ATG5. Our ATG5 antibody does not detect ATG12, which runs at about 23 kDa.
  4. Does this antibody detect free ATG5 monomer in HeLa or HEK 293 cells? 
    • This antibody is able to detect ATG5 (unconjugated form) at ~33 kDa and the ATG12-conjugated form at ~56 kDa. In the Western blot image we have on our website, we show the antibody's ability to detect the autophagy induced conjugate ATG5/ATG12.  A customer review image shows both the conjugated and unconjugated ATG5 in rat cardiomyocytes. Several  publications show this antibody working in Western blot with mouse and human samples. We are not sure if in any of the publications HeLa or HEK 293 cells specifically were used, but if the ATG5 protein is present, this product will detect it in Western blot.
  5. Would you please help confirm if NB110-53818 has been tested by IHC with frozen sections?
    • NB110-53818 has been tested with paraffin-embedded tissue, but we have not validated this antibody for IHC with frozen tissue sections.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for ATG5 Antibody (NB110-53818)

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Blogs on ATG5. Showing 1-10 of 32 blog posts - Show all blog posts.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.


  Read full blog post.


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  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.


  Read full blog post.

Autophagy independent roles of the core ATG proteins
By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation.  It is critical for removal of dam...  Read full blog post.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and ...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1, ...  Read full blog post.

Showing 1-10 of 32 blog posts - Show all blog posts.
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Recent Reviews

4.3
5
2
4
6
3
0
2
0
1
0

Armen Petrosyan
06/27/2023
Application: WB
Species: Human

Verified Customer
09/15/2020
Application: WB
Species: Mouse

 
Verified Customer
03/31/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ATG5