This LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Flow Cytometry and Western Blot where bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. Electron Microscopy was reported in scientific literature.
Read Publication using NB600-1384F in the following applications:
Zebrafish reactivity reported in scientific literature (PMID: 23724125). Canine and primate reactivity reported in scientific literature (PMID: 24027311) Porcine reactivity reported in scientific literature (PMID: 25378587) Other species have not been tested.
Packaging, Storage & Formulations
Store at 4C in the dark.
0.05% Sodium Azide
Immunogen affinity purified
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for LC3B Antibody [FITC]
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
lc3b autophagy marker
MAP1 light chain 3-like protein 2
MAP1A/MAP1B LC3 B
microtubule associated protein 3 b
microtubule associated protein 3 beta
microtubule-associated protein 1 light chain 3 betaMAP1A/MAP1B light chain 3 B
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably
associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
LC3B: From Autophagy to Cancer LC3B is subunit component of the LC3 autophagy biomarker associated with microtubule-associated proteins MAP1A and MAP1B and one of the best characterized markers to date. In resting state it is cytosolic, but upon activation, is lapidated and becomes... Read full blog post.
Analyzing LC3 in Western blot Microtubule-associated protein light chain 3 (LC3) is considered one of the definitive markers of autophagy, and its use is widespread in labs throughout the world. Despite its popularity, there are several considerations when employing LC3 antibodies... Read full blog post.
Marking the Autophagosome: the LC3 Antibody MAP1LC3 (shortened to LC3 in our antibody catalog) is one of four mammalian homologues of autophagy-related protein 8 (Atg8). It has been identified as a light chain subunit of the microtubule-associated proteins MAP1A/MAP1B. A modified form of LC3, L... Read full blog post.
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