GADD153/CHOP Antibody (9C8)

Images

 
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method ...read more
Flow Cytometry: GADD153/CHOP Antibody (9C8) [NB600-1335] - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody (9C8) NB600-1335 (blue) and a matched isotype control MAB004 (orange). Cells ...read more
Biological Strategies: Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
Biological Strategies: Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated ...read more
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method ...read more
Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Image shows a specific band for CHOP/GADD153 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, GS, ICC/IF, IHC, IHC-P, IP, ChIP, KD
Clone
9C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

GADD153/CHOP Antibody (9C8) Summary

Immunogen
Full length mouse CHOP/GADD153 [Swiss-Prot# P35639]
Epitope
The N-terminal region of CHOP/GADD153.
Localization
Nuclear
Marker
ER Stress Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DDIT3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Flow Cytometry
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Simple Western 1:250
  • Western Blot
Application Notes
Use in Knockdown Validated reported in scientific literature (PMID:32828953)In Western blot a band can be seen at approx. 29 Knockdown Validateda. Gel Super Shift Assays was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30962207). Use in ELISA reported in scientific literature (PMID: 29915575). Use in FLOW reported in scientific literature (PMID: 8650547).
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB600-1335 in the following applications:

Publications
Read Publications using
NB600-1335 in the following applications:

  • 1 publication
  • 1 publication
  • GS
    2 publications
  • 4 publications
  • 4 publications
  • IP
    4 publications
  • KD
    1 publication
  • WB
    23 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32828953). Human, mouse, rat and primate.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GADD153/CHOP Antibody (9C8)

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-06274
Species: Ce, Ch, Dr, Hu, Mu, Rt, Sh, Ze
Applications: Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, Simple Western, WB
NBP1-77681
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
AF1543
Species: Hu
Applications: IHC, WB
NBP1-40256
Species: Hu, Mu, Pl, Po, Rb, Rt
Applications: ChIP, CyTOF-ready, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, WB
NB100-852
Species: Hu, Mu
Applications: PEP-ELISA, WB
NB100-56708
Species: Ch, ChHa, Hu, Ma, Mu, Po, Rt
Applications: CyTOF-ready, EM, Flow, HEStain, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, WB
NBP1-80930
Species: Ch, Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
MAB17761
Species: Hu, Mu, Rt
Applications: ICC, WB
NBP2-45731
Species: Ca, Hu, Pm
Applications: IHC, IHC-P, WB
NBP2-67471
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-85699
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00152503-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB100-565
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KO, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB100-56618
Species: Hu, Mu
Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-P, WB
NBP3-05061
Species: Hu, Mu
Applications: ICC/IF, WB
NB600-1335
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, GS, ICC/IF, IHC, IHC-P, IP, ChIP, KD

Publications for GADD153/CHOP Antibody (NB600-1335)(37)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 8 applications: ChIP, ELISA, GS, ICC/IF, IHC-P, IP, KD, WB.


Filter By Application
ChIP
(1)
ELISA
(1)
GS
(2)
ICC/IF
(4)
IHC-P
(4)
IP
(4)
KD
(1)
WB
(23)
All Applications
Filter By Species
Human
(13)
Mouse
(18)
Rat
(2)
Primate
(1)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NB600-1335 Applications Species
Wu L, He S, Ye W Et al. Surf4 facilitates reprogramming by activating the cellular response to endoplasmic reticulum stress Cell proliferation Nov 1 2021 [PMID: 34585448] (WB, Mouse) WB Mouse
Zhao S, Chen W, Li W et al. LncRNA TUG1 attenuates ischaemia-reperfusion-induced apoptosis of renal tubular epithelial cells by sponging miR-144-3p via targeting Nrf2 Journal of cellular and molecular medicine Sep 21 2021 [PMID: 34547172] (WB, Mouse) WB Mouse
Fresia D, Cannizzaro E, Borgo A, Roduit R GSH-Independent Induction of ER Stress during Hypoglycaemia in the Retinal Cells of Mice Journal of Clinical Medicine Jun 7 2021 [PMID: 34200353] (WB, Mouse) WB Mouse
Zhang XT, Hu XB, Wang HL et al. Activation of unfolded protein response overcomes Ibrutinib resistance in diffuse large B-cell lymphoma Acta Pharmacol. Sin. Aug 27 2020 [PMID: 32855532]
Chen L, Xia YF, Shen SF et al. Syntaxin 17 inhibits ischemic neuronal injury by resuming autophagy flux and ameliorating endoplasmic reticulum stress Free Radic. Biol. Med. Aug 20 2020 [PMID: 32828953] (WB, KD, Mouse) WB, KD Mouse
Kim JL, Kim BR, Kim DY et al. Cannabidiol Enhances the Therapeutic Effects of TRAIL by Upregulating DR5 in Colorectal Cancer Cancers (Basel) May 9 2019 [PMID: 31075907] (IHC-P, Mouse) IHC-P Mouse
Anand S, Chatterjee N, Espinosa-Diez C A miR-494 dependent feedback loop regulates ER stress BioRxiv Jan 1 2020 (ICC/IF, Human) ICC/IF Human
Herranen A, IkAheimo K, Lankinen T et al. Deficiency of the ER-stress-regulator MANF triggers progressive outer hair cell death and hearing loss Cell Death Dis Feb 6 2020 [PMID: 32029702] (WB, Mouse) WB Mouse
Timberlake Ii M, Roy B, Dwivedi Y A Novel Animal Model for Studying Depression Featuring the Induction of the Unfolded Protein Response in Hippocampus Mol. Neurobiol. Jul 2 2019 [PMID: 31267370]
Nakada EM, Bhakta NR, Korwin-Mihavics BR et al. Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresposiveness by inhibiting UPR transducers JCI Insight May 2 2019 [PMID: 31045581] (WB, Mouse) WB Mouse
Show All 37 Publications.

Reviews for GADD153/CHOP Antibody (NB600-1335) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rat.

Reviews using NB600-1335:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot GADD153/CHOP NB600-1335
Enlarge
4
reviewed by:
Verified Customer
WB Human 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman liver and primary hepatocytes
SpeciesHuman
LotA-5

Comments

CommentsName: Anti-CHOP antibody (NB600-1335)
Catalog #: Anti-CHOP antibody (NB600-1335)
Lot Number: Anti-CHOP antibody (NB600-1335, Lot # A-5)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): lane 1: human liver; lane 2: human primary hepatocytes
Sample Information:
     Cell Line or Tissue: human liver and primary hepatocytes
     Species: human
     Treatment: No treatment
Lysate Preparation:
     Date of lysate preparation: December 9, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: No
     Negative Control: No
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 10%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/1000
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times
Secondary Antibody
Manufacturer and Catalog #: Promega, W402B, Lot # 0000191071
Secondary description: goat anti-mouse secondary antibody
     Dilution: 1/2000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot # 9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 40 seconds
Molecular weight of band(s): ~ 48 kDa
Experimental Concerns and Observations: A specific band around 48 kDa was observed
Western Blot GADD153/CHOP NB600-1335
Enlarge
4
reviewed by:
Wenliang Zhang
WB Rat 09/20/2017
View

Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesRat
LotA-2
Western Blot GADD153/CHOP NB600-1335
Enlarge
5
reviewed by:
Fabrice GANKAM
WB Rat 11/02/2014
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
CommentsSuprisingly detect 2 bands in rat brain at 16 and around 25 Kda, same pattern seen with antibodies from santacruz and sigma

Blocking

Blocking Detailsmilk 6% in PBST0.02% 2 hrs at RT

Primary Anitbody

Dilution Ratio1/2000 in PBST with milk 3% at 4 degres for 8 hrs

Secondary Antibody

Secondary Descriptiongoat anti mouse HRP rat cross adsorbed
Secondary Manufacturer Cat#Pierce
Secondary Concentration1/20000

Details

Detection NotesECL exposure time of 10 sec using Chemidoc XRS and biorad clarity

Comments

CommentsSuprisingly detect 2 bands in rat brain at 16 and around 25 Kda, same pattern seen with antibodies from santacruz and sigma

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GADD153/CHOP Antibody (NB600-1335) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GADD153/CHOP Products

Bioinformatics Tool for GADD153/CHOP Antibody (NB600-1335)

Discover related pathways, diseases and genes to GADD153/CHOP Antibody (NB600-1335). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GADD153/CHOP Antibody (NB600-1335)

Discover more about diseases related to GADD153/CHOP Antibody (NB600-1335).
 

Pathways for GADD153/CHOP Antibody (NB600-1335)

View related products by pathway.

PTMs for GADD153/CHOP Antibody (NB600-1335)

Learn more about PTMs related to GADD153/CHOP Antibody (NB600-1335).
 

Research Areas for GADD153/CHOP Antibody (NB600-1335)

Find related products by research area.

Blogs on GADD153/CHOP

There are no specific blogs for GADD153/CHOP, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Verified Customer
12/15/2017
Application: WB
Species: Human

Wenliang Zhang
09/20/2017
Application: WB
Species: Rat

Fabrice GANKAM
11/02/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol DDIT3
Entrez
Uniprot