Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - WB analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Western blot analysis of GADD153/CHOP expression in HeLa cells treated with 2.5ug/ml tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Figure 1 shows a Western blot of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with ...read more
Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Simple Western lane view shows a specific band for CHOP/GADD153 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
Protein A purified
This CHOP/GADD153 Antibody (9C8) is useful for Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band can be seen at ~29 kDa. Use in Rat reported in customer review. Gel Super Shift Assays was reported in scientific literature.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NB600-1335 in the following application:
Growth arrest and DNA damage-inducible protein GADD153
GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.