GADD153/CHOP Antibody (9C8)

Images

 
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method ...read more
Biological Strategies: Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
Biological Strategies: Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated ...read more
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method ...read more
Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Image shows a specific band for CHOP/GADD153 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, GS, ICC/IF, IHC, IHC-P, IP, ChIP
Clone
9C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

GADD153/CHOP Antibody (9C8) Summary

Immunogen
Full length mouse CHOP/GADD153 [Swiss-Prot# P35639]
Epitope
The N-terminal region of CHOP/GADD153.
Localization
Nuclear
Marker
ER Stress Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DDIT3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 1:250
  • ELISA
  • Flow Cytometry
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
In Western blot a band can be seen at approx. 29 kDa. Gel Super Shift Assays was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30962207). Use in ELISA reported in scientific literature (PMID: 29915575). Use in FLOW reported in scientific literature (PMID: 8650547).
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
GADD153/CHOP Knockout HeLa Cell Lysate
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB600-1335 in the following applications:

Publications
Read Publications using
NB600-1335 in the following applications:

  • 1 publication
  • 1 publication
  • GS
    2 publications
  • 3 publications
  • 4 publications
  • IP
    4 publications
  • WB
    18 publications

Reactivity Notes

Human, mouse, rat and primate.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GADD153/CHOP Antibody (9C8)

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-1335
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, GS, ICC/IF, IHC, IHC-P, IP, ChIP

Publications for GADD153/CHOP Antibody (NB600-1335)(30)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 7 applications: ChIP, ELISA, GS, ICC/IF, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
ELISA
(1)
GS
(2)
ICC/IF
(3)
IHC-P
(4)
IP
(4)
WB
(18)
All Applications
Filter By Species
Human
(11)
Mouse
(14)
Rat
(2)
Primate
(1)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB600-1335 Applications Species
Herranen A, IkAheimo K, Lankinen T et al. Deficiency of the ER-stress-regulator MANF triggers progressive outer hair cell death and hearing loss Cell Death Dis Feb 6 2020 [PMID: 32029702] (WB, Mouse) WB Mouse
Timberlake Ii M, Roy B, Dwivedi Y A Novel Animal Model for Studying Depression Featuring the Induction of the Unfolded Protein Response in Hippocampus Mol. Neurobiol. Jul 2 2019 [PMID: 31267370]
Kim JL, Kim BR, Kim DY et al. Cannabidiol Enhances the Therapeutic Effects of TRAIL by Upregulating DR5 in Colorectal Cancer Cancers (Basel) May 9 2019 [PMID: 31075907] (IHC-P, Mouse) IHC-P Mouse
Nakada EM, Bhakta NR, Korwin-Mihavics BR et al. Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresposiveness by inhibiting UPR transducers JCI Insight May 2 2019 [PMID: 31045581] (WB, Mouse) WB Mouse
Linden, M;Thomsen, C;Grundevik, P;Jonasson, E;Andersson, D;Runnberg, R;Dolatabadi, S;Vannas, C;Luna Santamaria, M;Fagman, H;Stahlberg, A;Aman, P; FET family fusion oncoproteins target the SWI/SNF chromatin remodeling complex EMBO Rep. Apr 8 2019 [PMID: 30962207] (ChIP, Human) ChIP Human
Nakandakari SCBR, Munoz VR, Kuga GK et al. Short-term high-fat diet modulates several inflammatory, ER stress, and apoptosis markers in the hippocampus of young mice Brain Behav. Immun. Feb 20 2019 [PMID: 30797044] (WB, Mouse) WB Mouse
Delbrel E, Soumare A, Naguez A et al. HIF-1a triggers ER stress and CHOP-mediated apoptosis in alveolar epithelial cells, a key event in pulmonary fibrosis Sci Rep Dec 18 2018 [PMID: 30560874] (WB, Human) WB Human
Siegert A, Garcia Diaz-Barriga G, Esteve-Codina A et al. A FBN1 3'UTR mutation variant is associated with endoplasmic reticulum stress in aortic aneurysm in Marfan syndrome Wound Repair Regen Oct 13 2018 [PMID: 30385411] (WB, Human) WB Human
Maciel M, HernAndez-Barrientos D, Herrera I et al. Impaired autophagic activity and ATG4B deficiency are associated with increased endoplasmic reticulum stress-induced lung injury. Aging (Albany NY). 2018 Aug 26 [PMID: 30147026] (IHC-P, Mouse) IHC-P Mouse
Cueno ME, Ochiai K Gingival Periodontal Disease (PD) Level-Butyric Acid Affects the Systemic Blood and Brain Organ: Insights Into the Systemic Inflammation of Periodontal Disease Front Immunol Jun 4 2018 [PMID: 29915575] (ELISA, Rat) Front Immunol. 2019 Apr 15 [PMID: 29915575] (ELISA, Rat)

Details:
Citation using the HRP form of this antibody.
ELISA Rat
Show All 30 Publications.

Reviews for GADD153/CHOP Antibody (NB600-1335) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rat.

Reviews using NB600-1335:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot GADD153/CHOP NB600-1335
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reviewed by:
Anonymous
WB Human 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman liver and primary hepatocytes
SpeciesHuman
LotA-5

Comments

CommentsName: Anti-CHOP antibody (NB600-1335)
Catalog #: Anti-CHOP antibody (NB600-1335)
Lot Number: Anti-CHOP antibody (NB600-1335, Lot # A-5)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): lane 1: human liver; lane 2: human primary hepatocytes
Sample Information:
     Cell Line or Tissue: human liver and primary hepatocytes
     Species: human
     Treatment: No treatment
Lysate Preparation:
     Date of lysate preparation: December 9, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: No
     Negative Control: No
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 10%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/1000
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times
Secondary Antibody
Manufacturer and Catalog #: Promega, W402B, Lot # 0000191071
Secondary description: goat anti-mouse secondary antibody
     Dilution: 1/2000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot # 9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 40 seconds
Molecular weight of band(s): ~ 48 kDa
Experimental Concerns and Observations: A specific band around 48 kDa was observed
Western Blot GADD153/CHOP NB600-1335
Enlarge
reviewed by:
Wenliang Zhang
WB Rat 09/20/2017
View

Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesRat
LotA-2
Western Blot GADD153/CHOP NB600-1335
Enlarge
reviewed by:
Fabrice GANKAM
WB Rat 11/02/2014
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
CommentsSuprisingly detect 2 bands in rat brain at 16 and around 25 Kda, same pattern seen with antibodies from santacruz and sigma

Blocking

Blocking Detailsmilk 6% in PBST0.02% 2 hrs at RT

Primary Anitbody

Dilution Ratio1/2000 in PBST with milk 3% at 4 degres for 8 hrs

Secondary Antibody

Secondary Descriptiongoat anti mouse HRP rat cross adsorbed
Secondary Manufacturer Cat#Pierce
Secondary Concentration1/20000

Details

Detection NotesECL exposure time of 10 sec using Chemidoc XRS and biorad clarity

Comments

CommentsSuprisingly detect 2 bands in rat brain at 16 and around 25 Kda, same pattern seen with antibodies from santacruz and sigma

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GADD153/CHOP Antibody (NB600-1335) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-1335AF350
Alexa Fluor 405 NB600-1335AF405
Alexa Fluor 488 NB600-1335AF488
Alexa Fluor 532 NB600-1335AF532
Alexa Fluor 594 NB600-1335AF594
Alexa Fluor 647 NB600-1335AF647
Alexa Fluor 700 NB600-1335AF700
Alexa Fluor 750 NB600-1335AF750
Biotin NB600-1335B
DyLight 350 NB600-1335UV
DyLight 405 NB600-1335V
DyLight 488 NB600-1335G
DyLight 550 NB600-1335R
DyLight 594 NB600-1335DL594
DyLight 650 NB600-1335C
DyLight 680 NB600-1335FR
DyLight 755 NB600-1335IR
FITC NB600-1335F
HRP NB600-1335H
Janelia Fluor 549 NB600-1335JF549
Janelia Fluor 646 NB600-1335JF646

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
12/15/2017
Application: WB
Species: Human

Wenliang Zhang
09/20/2017
Application: WB
Species: Rat

Fabrice GANKAM
11/02/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol DDIT3
Entrez
Uniprot
COVID-19 update