GADD153/CHOP Antibody (9C8)

Images

 
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - WB analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of mouse brain using 1:100 dilution of Calreticulin antibody. The ...read more
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Western blot analysis of GADD153/CHOP expression in HeLa cells treated with 2.5ug/ml tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Figure 1 shows a Western blot of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with ...read more
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of mouse brain using 1:100 dilution of Calreticulin antibody. The ...read more
Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Simple Western lane view shows a specific band for CHOP/GADD153 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
Clone
9C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

GADD153/CHOP Antibody (9C8) Summary

Immunogen
Full length mouse CHOP/GADD153 [Swiss-Prot# P35639]
Epitope
The N-terminal region of CHOP/GADD153.
Localization
Nuclear
Marker
ER Stress Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DDIT3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Simple Western 1:250
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This CHOP/GADD153 Antibody (9C8) is useful for Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band can be seen at approx. 29 kDa. Use in Rat reported in customer review. Gel Super Shift Assays was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB600-1335 in the following applications:

Publications
Read Publications using
NB600-1335 in the following applications:

  • GS
    2 publications
  • 3 publications
  • 2 publications
  • IP
    4 publications
  • WB
    10 publications

Reactivity Notes

Human, mouse, rat and primate.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GADD153/CHOP Antibody (9C8)

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP

Publications for GADD153/CHOP Antibody (NB600-1335)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Primate.

We have publications tested in 5 applications: GS, ICC/IF, IHC-P, IP, WB.


Filter By Application
GS
(2)
ICC/IF
(3)
IHC-P
(2)
IP
(4)
WB
(10)
All Applications
Filter By Species
Human
(7)
Mouse
(7)
Primate
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB600-1335 Applications Species
Jain A, Zode G, Kasetti RB et al. CRISPR-Cas9-based treatment of myocilin-associated glaucoma. Proc. Natl. Acad. Sci. U.S.A. 2017 Oct 17 [PMID: 28973933] (WB, ICC/IF, Human) WB, ICC/IF Human
Kilaparty SP, Agarwal R, Singh P et al. Endoplasmic reticulum stress-induced apoptosis accompanies enhanced expression of multiple inositol polyphosphate phosphatase 1 (Minpp1): a possible role for Minpp1 in cellular stress response. Cell Stress Chaperones. 2016 Apr 02 [PMID: 27038811] (WB, Mouse)

Details:
Mouse monoclonal CHOP antibody was used for WB analysis of lysates from MC3T3-E1 cells which were incubated or not with ER stress inducers brefeldin A and thapsigargin.
WB Mouse
Ying R, Wang XQ, Yang Y et al. Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway. Life Sci. 2016 Jan 01 [PMID: 26656263] (WB, Human) WB Human
Djelti F, Braudeau J, Hudry E et al. CYP46A1 inhibition, brain cholesterol accumulation and neurodegeneration pave the way for Alzheimer's disease Brain 2015 Aug 01 [PMID: 26141492] (IHC-P, Mouse)

Details:
CHOP antibody was used for IHC-P on brain tissues from wild-type C57Bl/6 and 5-month-old APP23 transgenic mice (Thy1-hAPPswe) that were subjected to injection of AAV-scramble or AAV-shCYP46A1 vectors. The IHC-P assay implicated 4% paraformaldehyde fixation at 4C for 24 hours, 6 um paraffin sections, sodium citrate based heat induced antigen retrieval, permeablization in PBS/0.1% Triton X 100, use of primary at 1:500 dilution with ON 4C incubation, detection using fluorescence labelled secondary (Figure 3A).
IHC-P Mouse
Lee YJ, Ha YJ, Na Kang Y et al. The Autophagy-Related Marker LC3 Can Predict Prognosis in Human Hepatocellular Carcinoma. PLoS One. 2013 Nov 25 [PMID: 24282606] (IHC-P, Human) IHC-P Human
Lin CJ, Lee CC, Shih YL et al. Inhibition of mitochondria- and endoplasmic reticulum stress-mediated autophagy augments temozolomide-induced apoptosis in glioma cells. PLoS One 2012 [PMID: 22745676] (WB, Human) WB Human
Hull RL, Zraika S, Udayasankar J, Aston-Mourney K, Subramanian SL, Kahn SE. Amyloid formation in human IAPP transgenic mouse islets and pancreas, and human pancreas, is not associated with endoplasmic reticulum stress. Diabetologia. 2009 Jun. [PMID: 19352619]
Kuroda M, Wang X, Sok J et al. Induction of a secreted protein by the myxoid liposarcoma oncogene. Proc Natl Acad Sci U S A. 1999 Apr 27. [PMID: 10220412]
Sok J, Wang XZ, Batchvarova N, Kuroda M, Harding H, Ron D. CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI. Mol Cell Biol;19(1):495-504. 1999 Jan. [PMID: 9858573] (WB) WB
Wang XZ, Harding HP, Zhang Y, Jolicoeur EM, Kuroda M, Ron D. Cloning of mammalian Ire1 reveals diversity in the ER stress responses. EMBO J;17(19):5708-17. 1998 Oct 1. [PMID: 9755171] (ICC/IF, Human, Primate) ICC/IF Human, Primate
Show All 16 Publications.

Reviews for GADD153/CHOP Antibody (NB600-1335) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Rat.
We have 2 reviews tested in 1 application: WB.

Reviews using NB600-1335:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot GADD153/CHOP NB600-1335
Enlarge
reviewed by:
Wenliang Zhang
WB Rat 09/20/2017
View

Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesRat
LotA-2
Western Blot GADD153/CHOP NB600-1335
Enlarge
reviewed by:
Fabrice GANKAM
WB Rat 11/02/2014
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
CommentsSuprisingly detect 2 bands in rat brain at 16 and around 25 Kda, same pattern seen with antibodies from santacruz and sigma

Blocking

Blocking Detailsmilk 6% in PBST0.02% 2 hrs at RT

Primary Anitbody

Dilution Ratio1/2000 in PBST with milk 3% at 4 degres for 8 hrs

Secondary Antibody

Secondary Descriptiongoat anti mouse HRP rat cross adsorbed
Secondary Manufacturer Cat#Pierce
Secondary Concentration1/20000

Details

Detection NotesECL exposure time of 10 sec using Chemidoc XRS and biorad clarity

Comments

CommentsSuprisingly detect 2 bands in rat brain at 16 and around 25 Kda, same pattern seen with antibodies from santacruz and sigma

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GADD153/CHOP Antibody (NB600-1335) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1335AF405
Alexa Fluor 488 Labeled NB600-1335AF488
Alexa Fluor 647 Labeled NB600-1335AF647
Alexa Fluor 700 Labeled NB600-1335AF700
Biotin Labeled NB600-1335B
DyLight 350 Labeled NB600-1335UV
DyLight 405 Labeled NB600-1335V
DyLight 488 Labeled NB600-1335G
DyLight 550 Labeled NB600-1335R
DyLight 650 Labeled NB600-1335C
DyLight 680 Labeled NB600-1335FR
DyLight 755 Labeled NB600-1335IR
FITC Labeled NB600-1335F
HRP Labeled NB600-1335H

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Wenliang Zhang
09/20/2017
Application: WB
Species: Rat

Fabrice GANKAM
11/02/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol DDIT3
Entrez
Uniprot