GADD153/CHOP Antibody (9C8)


Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - WB analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Western blot analysis of GADD153/CHOP expression in HeLa cells treated with 2.5ug/ml tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Figure 1 shows a Western blot of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with more
Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Simple Western lane view shows a specific band for CHOP/GADD153 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the more

Product Details

Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
1 mg/ml

GADD153/CHOP Antibody (9C8) Summary

Full length mouse CHOP/GADD153 [Swiss-Prot# P35639]
The N-terminal region of CHOP/GADD153.
ER Stress Marker
IgG2b Kappa
Protein A purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1 mg/ml
Protein A purified


Application Notes
This CHOP/GADD153 Antibody (9C8) is useful for Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band can be seen at ~29 kDa. Use in Rat reported in customer review. Gel Super Shift Assays was reported in scientific literature.In Simple Western only 10-15 uL of the recommended dilution is used per data point.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa
Reviewed Applications
Read 1 Review rated 5
NB600-1335 in the following application:

Read Publications using
NB600-1335 in the following applications:

  • GS
    2 publications
  • 2 publications
  • 2 publications
  • IP
    4 publications
  • WB
    9 publications

Reactivity Notes

Human, mouse, rat and primate.

Alternate Names for GADD153/CHOP Antibody (9C8)

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153


GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP

Publications for GADD153/CHOP Antibody (NB600-1335)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Primate.

We have publications tested in 5 applications: GS, ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB600-1335 Applications Species
Kilaparty SP, Agarwal R, Singh P et al. Endoplasmic reticulum stress-induced apoptosis accompanies enhanced expression of multiple inositol polyphosphate phosphatase 1 (Minpp1): a possible role for Minpp1 in cellular stress response. Cell Stress Chaperones. 2016 Apr 02 [PMID:27038811] (WB, Mouse) WB Mouse
Ying R, Wang XQ, Yang Y et al. Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway. Life Sci. 2016 Jan 01 [PMID:26656263] (WB, Human) WB Human
Djelti F, Braudeau J, Hudry E et al. CYP46A1 inhibition, brain cholesterol accumulation and neurodegeneration pave the way for Alzheimer's disease Brain 2015 Aug 01 [PMID:26141492] (IHC-P, Mouse) IHC-P Mouse
Lee YJ, Ha YJ, Na Kang Y et al. The Autophagy-Related Marker LC3 Can Predict Prognosis in Human Hepatocellular Carcinoma. PLoS One. 2013 Nov 25 [PMID:24282606] (IHC-P, Human) IHC-P Human
Lin CJ, Lee CC, Shih YL et al. Inhibition of mitochondria- and endoplasmic reticulum stress-mediated autophagy augments temozolomide-induced apoptosis in glioma cells. PLoS One 2012 [PMID:22745676] (WB, Human) WB Human
Hull RL, Zraika S, Udayasankar J, Aston-Mourney K, Subramanian SL, Kahn SE. Amyloid formation in human IAPP transgenic mouse islets and pancreas, and human pancreas, is not associated with endoplasmic reticulum stress. Diabetologia. 2009 Jun. [PMID:19352619]
Kuroda M, Wang X, Sok J et al. Induction of a secreted protein by the myxoid liposarcoma oncogene. Proc Natl Acad Sci U S A. 1999 Apr 27. [PMID:10220412]
Sok J, Wang XZ, Batchvarova N, Kuroda M, Harding H, Ron D. CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI. Mol Cell Biol;19(1):495-504. 1999 Jan. [PMID:9858573] (WB) WB
Wang XZ, Harding HP, Zhang Y, Jolicoeur EM, Kuroda M, Ron D. Cloning of mammalian Ire1 reveals diversity in the ER stress responses. EMBO J;17(19):5708-17. 1998 Oct 1. [PMID:9755171] (ICC/IF, Human, Primate) ICC/IF Human, Primate
Wang XZ, Kuroda M, Sok J, Batchvarova N, Kimmel R, Chung P, Zinszner H, Ron D. Identification of novel stress-induced genes downstream of chop. EMBO J;17(13):3619-30. 1998 Jul 1. [PMID:9649432] (WB, IP, Mouse) WB, IP Mouse
Show All 15 Publications.

Reviews for GADD153/CHOP Antibody (NB600-1335) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: WB.

Reviews using NB600-1335:
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Images Ratings Applications Species Date Details
Western Blot GADD153/CHOP NB600-1335
reviewed by:
WB Rat 11/02/2014


ApplicationWestern Blot
Sample TestedRat brain
CommentsSuprisingly detect 2 bands in rat brain at 16 and around 25 Kda, same pattern seen with antibodies from santacruz and sigma


Blocking Detailsmilk 6% in PBST0.02% 2 hrs at RT

Primary Anitbody

Dilution Ratio1/2000 in PBST with milk 3% at 4 degres for 8 hrs

Secondary Antibody

Secondary Descriptiongoat anti mouse HRP rat cross adsorbed
Secondary Manufacturer Cat#Pierce
Secondary Concentration1/20000


Detection NotesECL exposure time of 10 sec using Chemidoc XRS and biorad clarity


CommentsSuprisingly detect 2 bands in rat brain at 16 and around 25 Kda, same pattern seen with antibodies from santacruz and sigma

Product General Protocols

View specific protocols for GADD153/CHOP Antibody (NB600-1335): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GADD153/CHOP Antibody (NB600-1335) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-1335AF405
Alexa Fluor (R) 488 Labeled NB600-1335AF488
Alexa Fluor (R) 647 Labeled NB600-1335AF647
Alexa Fluor (R) 700 Labeled NB600-1335AF700
Allophycocyanin Labeled NB600-1335APC
Biotin Labeled NB600-1335B
DyLight 350 Labeled NB600-1335UV
DyLight 405 Labeled NB600-1335V
DyLight 405LS Labeled NB600-1335V3
DyLight 488 Labeled NB600-1335G
DyLight 550 Labeled NB600-1335R
DyLight 650 Labeled NB600-1335C
DyLight 680 Labeled NB600-1335FR
DyLight 755 Labeled NB600-1335IR
FITC Labeled NB600-1335F
HRP Labeled NB600-1335H
PE Labeled NB600-1335PE
PerCP Labeled NB600-1335PCP

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Contact Information

Product PDFs


Gene Symbol DDIT3

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