p62/SQSTM1 Antibody (2C11)

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of p62 in p62 WT and KO mouse hepatocytes and HepG2 cell lysate using anti-p62/SQSTM1 antibody clone 2C11. Sample lane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells. This image is from a verified customer product review.

Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - ICC-IF analysis of SQSTM1 protein in HeLa cell using SQSTM1/p62 antibody (clone 2C11) at 10 ug/ml concentration.

Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62 in SH-SY5Y cells using anti-p62/SQSTM1 antibody. Red - p62 puncta; Blue - nuclear DAPI; Green - Cytoskeleton. Image from verified customer review.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Disruption of the VANGL2-p62/SQSTM1 interaction in breast cancer cells. Soft agar colony formation of T47D cells overexpressing GFP, GFP-VANGL2 and GFP-p62/SQSTM1 (right). Protein expression was revealed with anti-p62/SQSTM1, anti-VANGL2 and anti-GFP antibodies by western blot analysis (right). In anti-GFP blot, the arrowhead indicates position of co-migrating GFP-VANGL2 and GFP-p62/SQSTM1 and the asterisk pinpoints GFP alone. Error bars represent mean+/-s.d. (n=3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms10318), licensed under a CC-BY licence.

ELISA: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Detection limit for recombinant GST tagged SQSTM1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: 2C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

p62/SQSTM1 Antibody (2C11) Summary

• Immunogen: SQSTM1 (AAH03139.1, 1 a.a. - 440 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
• Localization: Cytoplasmic
• Specificity: SQSTM1 - sequestosome 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SQSTM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA
  • Immunocytochemistry/Immunofluorescence 10ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:1000
• Application Notes: Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 23569437).
• Control:

  p62/SQSTM1 Knockout HeLa Cell Lysate
  p62/SQSTM1 Knockout 293T Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24352657). Human reactivity reported in scientific literature (PMID: 26571504). Rabbit reactivity reported in scientific literature (PMID: 23569437). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p62/SQSTM1 Antibody (2C11)

• A170
• EBI3-associated protein of 60 kDa
• EBI3-associated protein p60
• EBIAP
• ORCA
• OSIL
• oxidative stress induced like
• p60PDB3
• p62
• p62B
• Paget disease of bone 3
• PDB3
• phosphotyrosine independent ligand for the Lck SH2 domain p62
• Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
• Sequestosome 1
• sequestosome-1
• SQSTM1
• Ubiquitin-binding protein p62
• ZIP3

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in this gene result in sporadic and familial Paget disease of bone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p62/SQSTM1 Antibody (H00008878-M01)(232)

Publications using H00008878-M01

• Shojaei S, Koleini N, Samiei E et al. Simvastatin Increases Temozolomide-induced Cell Death by Targeting the Fusion of Autophagosomes and Lysosomes FEBS J. [PMID: 31545550]
• Wirth M, Zhang W, Razi M et al. Molecular determinants regulating selective binding of autophagy adapters and receptors to ATG8 proteins. Nat Commun. 2019 May 03 [PMID: 31053714]
• Roy, BC;Ahmed, I;Ramalingam, S;Jala, V;Haribabu, B;Ramamoorthy, P;Ashcraft, J;Valentino, J;Anant, S;Sampath, V;Umar, S; Co-localization of autophagy-related protein p62 with cancer stem cell marker dclk1 may hamper dclk1's elimination during colon cancer development and progression Oncotarget Mar 22 2019 [PMID: 31040926] (ICC/IF, Mouse)
• Park JS, Lee YS, Lee DH, Bae SH et al. Repositioning of niclosamide ethanolamine (NEN), an anthelmintic drug, for the treatment of lipotoxicity. Free Radic Biol Med. 2019 Apr 26 [PMID: 31035006]
• An, H;Ordureau, A;Paulo, JA;Shoemaker, CJ;Denic, V;Harper, JW; TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress Mol. Cell Apr 11 2019 [PMID: 31006537] (WB, Human)
• Sica V, Bravo-San Pedro JM, Izzo V et al. Lethal Poisoning of Cancer Cells by Respiratory Chain Inhibition plus Dimethyl alpha -Ketoglutarate. Cell Rep. 2019 Apr 16 [PMID: 30995479]
• Indrieri A, Carrella S, Romano A et al. miR-181a/b downregulation exerts a protective action on mitochondrial disease models. EMBO Mol Med. 2019 [PMID: 30979712]
• Wang B, Maxwell BA, Joo JH et al. ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Mol Cell. 2019 Apr 09 [PMID: 30979586]
• Zhao L, Pascual F, Bacudio L et al. Defective fatty acid oxidation in mice with muscle-specific acyl-CoA synthetase 1-deficiency increases amino acid use and impairs muscle function. J Biol Chem. 2019 Apr 11 [PMID: 30975900]
• Eid N, Ito Y, Horibe A et al. Ethanol-Induced Mitochondrial Damage in Sertoli Cells is Associated with Parkin Overexpression and Activation of Mitophagy Cells Mar 25 2019 [PMID: 30934625] (ICC/IF, Rat)
• Wang, X;Wu, WKK;Gao, J;Li, Z;Dong, B;Lin, X;Li, Y;Li, Y;Gong, J;Qi, C;Peng, Z;Yu, J;Shen, L; Autophagy inhibition enhances PD-L1 expression in gastric cancer J. Exp. Clin. Cancer Res. Mar 29 2019 [PMID: 30925913] (IHC-P, Human)
• Taisne C, Lussignol M, Hernandez E et al. Human cytomegalovirus hijacks the autophagic machinery and LC3 homologs in order to optimize cytoplasmic envelopment of mature infectious particles. Sci Rep. 2019 Mar 14 [PMID: 30872707]
• Vargas JNS, Wang C, Bunker E et al. Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy. Mol Cell. 2019 Mar 07 [PMID: 30853401]
• Carmona-Gutierrez D, Zimmermann A, Kainz K et al. The flavonoid 4,4'-dimethoxychalcone promotes autophagy-dependent longevity across species. Nat Commun. 2019 Feb 19 [PMID: 30783116]
• Horos R, Buscher M, Kleinendorst R et al. The Small Non-coding Vault RNA1-1 Acts as a Riboregulator of Autophagy Cell Feb 21 2019 [PMID: 30773316] (WB, Human)
• Li YP, Sin TK, Zhu JZ, Zhang G. p300 mediates muscle wasting in Lewis lung carcinoma Cancer Res. Jan 31 2019 [PMID: 30705122] (WB, Mouse)
• Feng X, Jia Y, Zhang Y et al. Ubiquitination of UVRAG by SMURF1 promotes autophagosome maturation and inhibits hepatocellular carcinoma growth. Autophagy Jan 27 2019 [PMID: 30686098] (WB)
• Koike K, Beatman EL, Schweitzer K et al. Subcutaneous administration of neutralizing antibodies to endothelial monocyte-activating protein II attenuates cigarette smoke-induced lung injury in mice. Am J Physiol Lung Cell Mol Phy 2019 Mar 05 [PMID: 30628489]
• Tooze SA Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-Throughput Screening. Methods Mol Biol. 2019 Feb 04 [PMID: 30610711]
• Stathakos P, Jimenez-Moreno N, Crompton L et al. Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research. Methods Mol Biol. 2019 Feb 04 [PMID: 30610703]
• Forrester A, De Leonibus C, Grumati P et al. A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex. EMBO J. 2019 Jan 03 [PMID: 30559329]
• Cai J, Pires KM, Ferhat M et al. Autophagy Ablation in Adipocytes Induces Insulin Resistance and Reveals Roles for Lipid Peroxide and Nrf2 Signaling in Adipose-Liver Crosstalk. Cell Rep Nov 13 2018 [PMID: 30428342]
• Rashid T, Nemazanyy I, Paolini C et al. Lipin1 deficiency causes sarcoplasmic reticulum stress and chaperone-responsive myopathy. EMBO J. 2018 Nov 12 [PMID: 30420558]
• Sun F, Jiang X, Wang X et al. Vincristine Ablation of Sirt2 Induces Cell Apoptosis and Mitophagy via Hsp70 Acetylation in MDA-MB-231 Cells. Biochem Pharmacol. 2018 Oct 21 [PMID: 30352233]
• Janssen AFJ, Katrukha EA, van Straaten W et al. Probing aggrephagy using chemically-induced protein aggregates. Nat Commun Oct 12 2018 [PMID: 30315152]
• Winer H, Fraiberg M, Abada A et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Nat Commun Sep 14 2018 [PMID: 30218067]
• Siddique T, Chen W, Deng H et al. Molecular Targets for ALS and Related Disorders. United States Patent Application. 2016
• Zhang Y, Mun SR, Linares JF et al. ZZ-dependent regulation of p62/SQSTM1 in autophagy. Nat Commun. Oct 22 2018 [PMID: 30349045] (WB, Human)
• Wei YM, Han B., et al. Beclin1 decreases the RIPA-insoluble fraction of amyotrophic lateral sclerosis-linked SOD1 mutant via autophagy. Neurosci Lett Oct 12 2018 [PMID: 30321575]
• Civiletto G, Dogan SA, Cerutti R et al. Rapamycin rescues mitochondrial myopathy via coordinated activation of autophagy and lysosomal biogenesis. EMBO Mol Med Nov 15 2018 [PMID: 30309855]
• Fahmy AM, Khabir M, Blanchet M et al. LC3B is not recruited along with the autophagy elongation complex (ATG5-12/16L1) at HCV replication site and is dispensable for viral replication. PLoS One Oct 4 2018 [PMID: 30286180]
• Kim J, Jeon T, Baek K et al. Effects of 10-Week Treadmill Exercise on the Improvement of Obesity-Induced Autophagy Dysregulation in Arterial Endothelial Cells of Mice Exerc Sci Aug 31 2019 (IB, Mouse)
• Ashraf N, Duarte-Silva S, Shaw E et al. Citalopram Reduces Aggregation of ATXN3 in a YAC Transgenic Mouse Model of Machado-Joseph Disease. Mol. Neurobiol. Sep 4 2018 [PMID: 30187384] (WB, Mouse)
• Brochard L Fatty acid-induced stress signalling in adipocytes Thesis Jul 11 1905 (WB, Human)
• Mattar P, Bravo-Sagua R, Tobar N et al. Autophagy mediates calcium-sensing receptor-induced TNFa production in human preadipocytes Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Nov 1 2018 [PMID: 30251678] (WB, Human)
• Lin CW, Chen YS, Lin CC et al. Significant predictors of overall survival in patients with hepatocellular carcinoma after surgical resection. PLoS One Sep 4 2018 [PMID: 30180193]
• Palomo GM, Granatiero V, Kawamata H et al. Parkin is a disease modifier in the mutant SOD1 mouse model of ALS EMBO Mol Med Aug 20 2018 [PMID: 30126943] (WB, IHC, Mouse)
• Dogan SA, Cerutti R, Beninca C et al. Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy. Cell Metab Aug 9 2018 [PMID: 30122554]
• Sonigo C, Beau I, Grynberg M et al. AMH prevents primordial ovarian follicle loss and fertility alteration in cyclophosphamide-treated mice. FASEB J Aug 16 2018 [PMID: 30113879]
• Ma S, Chen X, Tan Q et al. An Engineered DC-Targeting Lentivector Induces Robust T Cell Responses and Inhibits HBV Replication in HBV Transgenic Mice via Upregulating T Cell Autophagy. Cell Physiol Biochem Jul 24 2018 [PMID: 30041239]
• Schweitzer GG, Collier SL, Chen Z et al. Loss of lipin 1-mediated phosphatidic acid phosphohydrolase activity in muscle leads to skeletal myopathy in mice. FASEB J. Jul 20 2018 [PMID: 30028636] (IHC-Fr, Mouse)
• Manzella N, Santin Y, Maggiorani D et al. Monoamine oxidase-A is a novel driver of stress-induced premature senescence through inhibition of parkin-mediated mitophagy. Aging Cell Jul 12 2018 [PMID: 30003648]
• Henderson MX, Peng C, Trojanowski JQ, Lee VMY. LRRK2 activity does not dramatically alter alpha-synuclein pathology in primary neurons. Acta Neuropathol Commun 2018 May 31 [PMID: 29855356] (Human)
• Li Y, Chao X, Yang L et al. Impaired Fasting-Induced Adaptive Lipid Droplet Biogenesis in Liver-Specific Atg5-Deficient Mouse Liver Is Mediated by Persistent Nuclear Factor-Like 2 Activation. Am J Pathol May 25 2018 [PMID: 29803835]
• Ohri SS, Bankston AN, Mullins SA et al. Blocking Autophagy in Oligodendrocytes Limits Functional Recovery after Spinal Cord Injury. J Neurosci May 23 2018 [PMID: 29793971]
• Chao X, Wang S, Zhao K et al. Impaired TFEB-mediated Lysosome Biogenesis and Autophagy Promote Chronic Ethanol-induced Liver Injury and Steatosis in Mice. Gastroenterology May 18 2018 [PMID: 29782848]
• Liu ZQ, Lee JN, Son M et al. Ciliogenesis is reciprocally regulated by PPARA and NR1H4/FXR through controlling autophagy in vitro and in vivo. Autophagy May 17 2018 [PMID: 29771182]
• Lin KC, Lin MW, Hsu MN et al. Graphene oxide sensitizes cancer cells to chemotherapeutics by inducing early autophagy events, promoting nuclear trafficking and necrosis. Theranostics Mar 28 2018 [PMID: 29721093]
• Pascall JC, Webb LMC, Eskelinen EL et al. GIMAP6 is required for T cell maintenance and efficient autophagy in mice. PLoS One May 2 2018 [PMID: 29718959]
• Aveic S, Pantile M, Polo P et al. Autophagy inhibition improves the cytotoxic effects of receptor tyrosine kinase inhibitors. Cancer Cell Int Apr 24 2018 [PMID: 29713246]
• Gouspillou G, Godin R, Piquereau J et al. Protective role of Parkin in skeletal muscle contractile and mitochondrial function. J Physiol Apr 22 2018 [PMID: 29682760]
• Maharjan Y, Lee JN, Kwak S et al. Autophagy alteration prevents primary cilium disassembly in RPE1 cells. Biochem Biophys Res Commun Apr 13 2018 [PMID: 29649478]
• Duarte-Silva S, Neves-Carvalho A, Soares-Cunha C et al. Neuroprotective effects of creatine in the CMVMJD135 mouse model of spinocerebellar ataxia type 3. Mov Disord Mar 23 2018 [PMID: 29570846]
• Khan W, Layden BT, Chakrabarti P. Inhibition of mTOR complexes protects cancer cells from glutamine starvation induced cell death by restoring Akt stability. Biochim. Biophys. Acta. Mar 16 2018 [PMID: 29555211] (WB)
• Bresciani A, Spiezia MC, Boggio R et al. Quantifying autophagy using novel LC3B and p62 TR-FRET assays. PLoS One 2018 Mar 19 [PMID: 29554128]
• Thomas-Jardin SE, Kanchwala MS, Jacob J et al. Identification of an IL-1-induced gene expression pattern in AR(+) PCa cells that mimics the molecular phenotype of AR(-) PCa cells. Prostate 2018 Jun [PMID: 29527701]
• Dodson M, Liu P, Jiang T et al. Increased O-GlcNAcylation of SNAP29 drives arsenic-induced autophagic dysfunction. Mol Cell Biol Mar 5 2018 [PMID: 29507186]
• Bermejo-Millo JC, Guimaraes MRM, de Luxan-Delgado B et al. High-Fructose Consumption Impairs the Redox System and Protein Quality Control in the Brain of Syrian Hamsters: Therapeutic Effects of Melatonin. Mol Neurobiol Feb 28 2018 [PMID: 29492847]
• Ordonez-Gutierrez L, Benito-Cuesta I, Abad JL, Casas J. Dihydroceramide Desaturase 1 Inhibitors Reduce Amyloid-b Levels in Primary Neurons from an Alzheimer's Disease Transgenic Model. Pharm. Res. 2018 Feb 06 [PMID: 29411122] (Mouse)
• Brewer RA, Collins HE, Berry RD et al. Temporal partitioning of adaptive responses of the murine heart to fasting. Life Sci Jan 30 2018 [PMID: 29410090]
• Dodson M, de la Vega MR, Harder B et al. Low-level arsenic causes proteotoxic stress and not oxidative stress. Toxicol Appl Pharmacol Feb 3 2018 [PMID: 29408041]
• Koukourakis MI, Giatromanolaki A, Fylaktakidou K, Sivridis E. SMER28 is a mTOR-independent small molecule enhancer of autophagy that protects mouse bone morrow and liver against radiotherapy. Invest New Drugs. 2018 Jan 31 [PMID: 29387992] (WB, Mouse)
• Gstrein T, Edwards A, PristoupilovA A et al. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nat. Neurosci. Feb 1 2018 [PMID: 29311744] (WB, Human)
• Koukourakis MI, Giatromanolaki A, Fylaktakidou K et al. Amifostine Protects Mouse Liver Against Radiation-induced Autophagy Blockage. Anticancer Res. 2018 Jan 01 [PMID: 29277777] (WB, Mouse)
• An H, Harper JW. Systematic analysis of ribophagy in human cells reveals bystander flux during selective autophagy. Nat. Cell Biol. 2017 Dec 11 [PMID: 29230017] (Human)
• Balbi T, Cortese K, Ciacci C et al. Autophagic processes in Mytilus galloprovincialis hemocytes: Effects of Vibrio tapetis. Fish Shellfish Immunol Dec 5 2017 [PMID: 29208501]
• Cevatemre B, Erk?sa M, Aztopal N et al. A promising natural product, pristimerin, results in cytotoxicity against breast cancer stem cells in vitro and xenografts in vivo through apoptosis and an incomplete autopaghy in breast cancer. Pharmacol Res Dec 1 2017 [PMID: 29197639]
• Baron O, Boudi A, Dias C et al. Stall in Canonical Autophagy-Lysosome Pathways Prompts Nucleophagy-Based Nuclear Breakdown in Neurodegeneration. Curr Biol Nov 17 2017 [PMID: 29174892]
• Rubio-Gonzalez A, Bermejo-Millo JC, de Luxan-Delgado B et al. Melatonin Prevents the Harmful Effects of Obesity on the Brain, Including at the Behavioral Level. Mol Neurobiol Oct 30 2017 [PMID: 29086246]
• Thiessen SE, Derese I, Derde S et al. The Role of Autophagy in Critical Illness-induced Liver Damage. Sci Rep. 2017 Oct 26 [PMID: 29074879] (WB, Mouse)
• Bartolomeo R, Cinque L, De Leonibus C et al. mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest Sep 5 2017 [PMID: 28872463]
• Tuncer S, Banerjee S. Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells. Methods Mol Biol 2017 Aug 27 [PMID: 28842896]
• Zhang X, Wu WK, Xu W et al. CXC Motif Chemokine 10 Impairs Autophagy and Autolysosome Formation in Non-alcoholic Steatohepatitis Theranostics. [PMID: 28824718] (WB, Human)
• Rocchi A, Yamamoto S, Ting T et al. A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease. PLoS Genet 2017 Aug 14 [PMID: 28806762]
• Liang F, Li T, Azuelos I et al. Ventilator-induced diaphragmatic dysfunction in mdx mice Muscle Nerve 2017 Aug 08 [PMID: 28786111] (Mouse)
• Garcia-Sanz P, Orgaz L, Bueno-Gil G et al. N370S-GBA1 mutation causes lysosomal cholesterol accumulation in Parkinson's disease. Mov Disord 2017 Aug 5 [PMID: 28779532]
• Benitez BA, Sands MS. Primary fibroblasts from CSPa mutation carriers recapitulate hallmarks of the adult onset neuronal ceroid lipofuscinosis. Sci Rep 2017 Jul 24 [PMID: 28740222]
• McGinnis GR, Tang Y, Brewer RA et al. Genetic disruption of the cardiomyocyte circadian clock differentially influences insulin-mediated processes in the heart. J Mol Cell Cardiol 2017 Jul 19 [PMID: 28736261]
• Santarino IB, Viegas MS, Domingues NS et al. Involvement of the p62/NRF2 signal transduction pathway on erythrophagocytosis Sci Rep 2017 Jul 19 [PMID: 28724916]
• Joachim J, Razi M, Judith D et al. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Curr Biol 2017 Jul 14 [PMID: 28712572]
• Willy JA, Young SK, Mosley AL et al. Function of Inhibitor of Brutons Tyrosine Kinase Isoform alpha (IBTKalpha) in Nonalcoholic Steatohepatitis links Autophagy and the Unfolded Protein Response J. Biol. Chem. 2017 Jul 14 [PMID: 28710282] (WB, Human)
• Ko SH, Jeon JI, Myung HS et al. Bacteroides fragilis Enterotoxin Induces Formation of Autophagosomes in Endothelial Cells but Interferes with Fusion with Lysosomes for Complete Autophagic Flux through a Mitogen-Activated Protein Kinase-, AP-1-, and C/EBP Homologous Protein-Dependent Pat Infect Immun 2017 Jul 10 [PMID: 28694294]
• Henderson MX, Chung CH, Riddle DM et al. Unbiased proteomics of early Lewy body formation model implicates active microtubule affinity-regulating kinases (MARKs) in synucleinopathies. J Neurosci 2017 May 18 [PMID: 28522732]
• Akio Horibe, Nabil Eid, Yuko Ito et al. Upregulated Autophagy in Sertoli Cells of Ethanol-Treated Rats Is Associated with Induction of Inducible Nitric Oxide Synthase (iNOS), Androgen Receptor Suppression and Germ Cell Apoptosis. Int J Mol Sci. [PMID: 28505146] (WB, ICC/IF, Rat)
• Schnettger L, Rodgers A, Repnik U et al. A Rab20-Dependent Membrane Trafficking Pathway Controls M. tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity. Cell Host Microbe 2017 May 10 [PMID: 28494243]
• Orcholski ME, khurshudYan A, Shamskhou EA et al. Reduced carboxylesterase 1 is associated with endothelial injury in methamphetamine-induced pulmonary arterial hypertension. Am. J. Physiol. Lung Cell Mol. Physiol. Aug 1 2017 [PMID: 28473326] (WB, Human)
• Lee EB, Porta S, Michael Baer G et al. Expansion of the classification of FTLD-TDP: distinct pathology associated with rapidly progressive frontotemporal degeneration. Acta Neuropathol 2017 Jan 27 [PMID: 28130640]
• Redmann M, Wani WY, Volpicelli-Daley L et al. Trehalose does not improve neuronal survival on exposure to alpha-synuclein pre-formed fibrils. Redox Biol 2017 Jan 3 [PMID: 28068606]
• Zhou Y, Li Y, Ni HM et al. Nrf2 but not autophagy inhibition is associated with the survival of wild-type epidermal growth factor receptor non-small cell lung cancer cells. Toxicology and Applied Pharmacology Sep 14 2016
• Covell DJ, Robinson JL, Akhtar RS et al. Novel conformation-selective alpha-synuclein antibodies raised against different in vitro fibril forms show distinct patterns of Lewy pathology in Parkinson's disease. Neuropathol Appl Neurobiol 2017 Apr 7 [PMID: 28386933]
• Zhang Y, Nguyen DT, Olzomer EM et al. Rescue of Pink1 Deficiency by Stress-Dependent Activation of Autophagy. Cell Chem Biol 2017 Mar 30 [PMID: 28366621]
• Rodriguez-Arribas M, Yakhine-Diop SMS, Gonzalez-Polo RA et al. Turnover of Lipidated LC3 and Autophagic Cargoes in Mammalian Cells. Methods in Enzymology 2016 Jan 12 [PMID: 28253976]
• Panayotopoulou EG, Muller AK, Borries M et al. Targeting of apoptotic pathways by SMAC or BH3 mimetics distinctly sensitizes paclitaxel-resistant triple negative breast cancer cells Oncotarget 2017 Jul 11 [PMID: 28187446] (Human)
• Ma X, Zhang S, He L et al. MTORC1-mediated NRBF2 phosphorylation functions as a switch for the class III PtdIns3K and autophagy Autophagy 2017 Mar 04 [PMID: 28059666] (WB)
• Haldar AK, Piro AS, Finethy R et al. Chlamydia trachomatis Is Resistant to Inclusion Ubiquitination and Associated Host Defense in Gamma Interferon-Primed Human Epithelial Cells. MBio 2016 Dec 13 [PMID: 27965446]
• Redmann M, Benavides GA, Berryhill TF et al. Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons. Redox Biol 2016 Nov 18 [PMID: 27889640]
• Sambri I, D'Alessio R, Ezhova Y et al. Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases. EMBO Mol Med 2017 Jan [PMID: 27881461]
• Aydinlik S, Erkisa M, Cevatemre B et al. Enhanced cytotoxic activity of doxorubicin through the inhibition of autophagy in triple negative breast cancer cell line. Biochim Biophys Acta 2016 Nov 11 [PMID: 27842219]
• Zhou Y, Li Y, Ni HM et al. Nrf2 but not autophagy inhibition is associated with the survival of wild-type epidermal growth factor receptor non-small cell lung cancer cells. Toxicol Appl Pharmacol 2016 Nov 01 [PMID: 27639429] (Human)
• Conlon DM, Thomas T, Fedotova T et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J Clin Invest 2016 Sep 6 [PMID: 27599291]
• Gong J, Belinsky G, Sagheer U et al. Pigment Epithelium-derived Factor (PEDF) Blocks Wnt3a-induced Autophagy in Pancreatic Intraepithelial Neoplasms. J. Biol. Chem. Aug 24 2016 [PMID: 27557659] (IHC, Mouse)
• Wilson CH, Nikolic A, Kentish SJ et al. Sex-specific alterations in glucose homeostasis and metabolic parameters during ageing of caspase-2-deficient mice. Cell Death Discov 2016 Feb 29 [PMID: 27551503] (WB)
• Jin HO, Hong SE, Park JA et al. Inhibition of JNK-mediated autophagy enhances NSCLC cell sensitivity to mTORC1/2 inhibitors. Sci Rep 2016 Jun 30 [PMID: 27358039] (WB)
• Morgan MJ, Thorburn A. Measuring Autophagy in the Context of Cancer. Adv Exp Med Biol 2016 [PMID: 27325265]
• Li Y, McGreal S, Zhao J et al. A Cell-Based Quantitative High-Throughput Image Screening Identified Novel Autophagy Modulators. Pharmacol Res 2016 May 7 [PMID: 27168224]
• Seiferling D, Szczepanowska K, Becker C et al. Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt. EMBO Rep 2016 May 6 [PMID: 27154400]
• de Luxan-Delgado B, Potes Y, Rubio-Gonzalez A et al. Melatonin reduces endoplasmic reticulum stress and autophagy in liver of leptin-deficient mice. J Pineal Res 2016 Aug [PMID: 27090356]
• He L, Hamm JA, Reddy A et al. Biotinylation: a novel posttranslational modification linking cell autonomous circadian clocks with metabolism. Am J Physiol Heart Circ Physiol 2016 Jun 1 [PMID: 27084392]
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  Details:
  p62/SQSTM1 antibody used for WB on lysates of transversus abdominis muscle / TA muscles from mice that were fed with regular chow or fasted for 3-days (Figure 2B)
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Reviews for p62/SQSTM1 Antibody (H00008878-M01) (8)

Average Rating: 4.5

(Based on 8 reviews)

We have 8 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Anonymous WB Human 08/17/2019

Summary

• Application: Western Blot
• Sample Tested: HEK293
• Species: Human
• Lot: H5191-2C11

Comments

• Comments: 60ug of total cell lysate was loaded for the KO screening. There were several non-specific bands below 50Kda region.

reviewed by: Anonymous WB Mouse 12/18/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse embryonic fibroblasts
• Species: Mouse
• Lot: H5191-2C11

Comments

• Comments: p62 knockout mouse embryonic fibroblasts
  Name: Anti-p62 antibody (H00008878-M01)
  Catalog #: Anti-p62 antibody (H00008878-M01)
  Lot Number: Anti-p62 antibody (H00008878-M01, Lot # H5191-2C11)
  PO/Order Number: Click here to enter text..
  WB Image Description (Please provide labels for all lanes): lane 1: WT mouse embryonic fibroblasts; lane 2: p62 knockout mouse embryonic fibroblasts
  Sample Information:
       Cell Line or Tissue: mouse embryonic fibroblasts;
       Species: mouse
       Treatment: No treatment
  Lysate Preparation:
       Date of lysate preparation: December 15, 2017
       Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
  Reducing agent: beta-mercaptoethanol, DTT
  If boiled (temperature/time): Yes
  Controls:
       Positive Control: Wild type
       Negative Control: Knockout
       Loading Control (please attach additional images if applicable): No
  Protein Amount Loaded per lane: 20 ug
  Antibody Storage Conditions: -20℃
  Electrophoresis:
  Gel Percentage: 8%
  Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
       Voltage: 120V
       Time: 2 hours
  Membrane Transfer:
       Method (Submersion/Semi-dry): wet transfer
       Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
       Time: 2 hours
       Voltage: 100V
  Blocking:
       Blocking Solution: 5% milk in 1X TBST
       Time: 1 hour at room temperature
  Primary Antibody:
       Dilution: 1/1000
       Diluent Buffer: 2.5% BSA
       Incubation Time: overnight
       Incubation Temperature: 4℃
  Washing Conditions:
       Wash Solution: 1X TBST
       Time and Repetitions: 5 min each for 3 times
  
  Secondary Antibody
  Manufacturer and Catalog #: Promega, W402B, Lot # 0000191071
  Secondary description: goat anti-mouse secondary antibody
       Dilution: 1/2000
       Diluent Buffer: 3% milk
       Incubation Time: 1 hour
       Incubation Temperature: room temperature
  Detection Method:
  Detection: ECL (GE, cat # RPN2209, lot # 9838243)
  Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
  Development Time: 20 seconds
  Molecular weight of band(s): ~ 65 kDa
  Experimental Concerns and Observations: A specific band around 65 kDa was observed

reviewed by: Woojong Lee WB Mouse 05/04/2017

Summary

• Application: Western Blot
• Sample Tested: DEndritic cell lysate
• Species: Mouse

reviewed by: Anonymous IF Human 04/05/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Differentiated cell line
• Species: Human

Blocking

• Blocking Details: Blocking with 2%FBS for 30min

Primary Anitbody

• Dilution Ratio: 1:200 in PBS 1x

Secondary Antibody

• Secondary Description: Alexa Fluor 546 donkey-anti-mouse
• Secondary Concentration: 1:1000

Details

• Detection Notes: Fluorescence Microscope
• Fixation Details: Fixation in 4% PFA for 20min

reviewed by: Fabrice GANKAM WB Rat 11/01/2014

Summary

• Application: Western Blot
• Sample Tested: Rat brain
• Species: Rat
• Comments: Excellent antibody. detect 2 bands though at the right MW. too bad I cut me membrane :-)

Blocking

• Blocking Details: Milk 6% in PBST0.02% for 1hr RT

Primary Anitbody

• Dilution Ratio: 1/5000 for 8 hrs at 4degrees

Secondary Antibody

• Secondary Description: anti rabbit HRP
• Secondary Manufacturer Cat#: Pierce thermoscientific
• Secondary Concentration: 1/10000

Details

• Detection Notes: ECL (biorad clarity) with biorad chemidoc exposure time 10 sec

Comments

• Comments: Excellent antibody. detect 2 bands though at the right MW. too bad I cut me membrane :-)

reviewed by: Anonymous WB Mouse 10/24/2014

Summary

• Application: Western Blot
• Sample Tested: mouse hepatocytes and HepG2 cells
• Species: Mouse

Blocking

• Blocking Details: 6% Non-fat milk in TBS at 4C for overnight

Primary Anitbody

• Dilution Ratio: 1/1000 dilution in 3% non-fat milk in TBST (0.1% tween) at room temp for 1hr

Secondary Antibody

• Secondary Description: Rabbit Anti-Mouse HRP (IgG H&L)
• Secondary Manufacturer Cat#: biorad #172-1011
• Secondary Concentration: 1:10000 dilution

Details

• Detection Notes: lane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells

reviewed by: Nadine Bakkar IP Human 08/07/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: Human spinal cord cytoplasmic homogenates
• Species: Human
• Comments: Interaction between KEAP and p62/sqstm is previously reported.

Blocking

• Blocking Details: LIcoR blocking buffer

Primary Anitbody

• Dilution Ratio: Rb KEAP from PRoteintech

Secondary Antibody

• Secondary Description: anti-rabbit secondary antibody from LicoR, IRdye 800
• Secondary Manufacturer Cat#: LicoR
• Secondary Concentration: 1:20000

Details

• Detection Notes: LicoR Odyssey
• Immunoprecipitation Details: Protein A/G agarose, Overnight incubation in 1%NP-40, Washes in RIPA
• Elution Description: 4xSDS dye, 5min boiling.

Comments

• Comments: Interaction between KEAP and p62/sqstm is previously reported.

reviewed by: Lin Zhang WB Mouse 09/28/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000

Bioinformatics

• Gene Symbol: SQSTM1
• Entrez:
  • 8878(Human)
  • 18412(Mouse)
• Uniprot:
  • Q13501()