p62/SQSTM1 Antibody (2C11)

Images

 
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of p62 in p62 WT and KO mouse hepatocytes and HepG2 cell lysate using anti-p62/SQSTM1 antibody clone 2C11. Sample lane1: p62 +/- mouse hepatocytes, ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - ICC-IF analysis of SQSTM1 protein in HeLa cell using SQSTM1/p62 antibody (clone 2C11) at 10 ug/ml concentration.
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62 in SH-SY5Y cells using anti-p62/SQSTM1 antibody. Red - p62 puncta; Blue - nuclear DAPI; Green - Cytoskeleton. Image ...read more
ELISA: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Detection limit for recombinant GST tagged SQSTM1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
Clone
2C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
     

Genetic Strategies

   

Order Details

p62/SQSTM1 Antibody (2C11) Summary

Immunogen
SQSTM1 (AAH03139.1, 1 a.a. - 440 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Localization
Cytoplasmic
Specificity
SQSTM1 - sequestosome 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SQSTM1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Reviewed Applications
Read 6 Reviews rated 4.7
using
H00008878-M01 in the following applications:

Publications
Read Publications using
H00008878-M01 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24352657). Human reactivity reported in scientific literature (PMID: 26571504). Rabbit reactivity reported in scientific literature (PMID: 23569437). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p62/SQSTM1 Antibody (2C11)

  • A170
  • EBI3-associated protein of 60 kDa
  • EBI3-associated protein p60
  • EBIAP
  • ORCA
  • OSIL
  • oxidative stress induced like
  • p60PDB3
  • p62
  • p62B
  • Paget disease of bone 3
  • PDB3
  • phosphotyrosine independent ligand for the Lck SH2 domain p62
  • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
  • Sequestosome 1
  • sequestosome-1
  • SQSTM1
  • Ubiquitin-binding protein p62
  • ZIP3

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in this gene result in sporadic and familial Paget disease of bone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p62/SQSTM1 Antibody (H00008878-M01)(146)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(7)
IHC
(3)
IHC-Fr
(1)
IHC-P
(1)
IP
(1)
WB
(42)
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Filter By Species
Human
(22)
Mouse
(22)
Rat
(5)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 146. Show All 146 Publications.
Publications using H00008878-M01 Applications Species
Thiessen SE, Derese I, Derde S et al. The Role of Autophagy in Critical Illness-induced Liver Damage. Sci Rep. 2017 Oct 26 [PMID: 29074879] (WB, Mouse) WB Mouse
Zhang X, Wu WK, Xu W et al. CXC Motif Chemokine 10 Impairs Autophagy and Autolysosome Formation in Non-alcoholic Steatohepatitis THERANOSTICS 2017 Aug 21 [PMID: 28824718] (WB, Human) WB Human
Zhang X, Wu WK, Xu W et al. C-X-C Motif Chemokine 10 Impairs Autophagy and Autolysosome Formation in Non-alcoholic Steatohepatitis Theranostics 2017 Aug 21 [PMID: 28824718] (WB, Human) WB Human
Liang F, Li T, Azuelos I et al. Ventilator-induced diaphragmatic dysfunction in mdx mice Muscle Nerve 2017 Aug 08 [PMID: 28786111] (Mouse) Mouse
Willy JA, Young SK, Mosley AL et al. Function of Inhibitor of Brutons Tyrosine Kinase Isoform alpha (IBTKalpha) in Nonalcoholic Steatohepatitis links Autophagy and the Unfolded Protein Response J. Biol. Chem. 2017 Jul 14 [PMID: 28710282] (WB, Human) WB Human
Akio Horibe, Nabil Eid, Yuko Ito et al. Upregulated Autophagy in Sertoli Cells of Ethanol-Treated Rats Is Associated with Induction of Inducible Nitric Oxide Synthase (iNOS), Androgen Receptor Suppression and Germ Cell Apoptosis. International Journal of Molecular Sciences 2017 May 15 [PMID: 28505146] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Akio Horibe, Nabil Eid, Yuko Ito et al. Upregulated Autophagy in Sertoli Cells of Ethanol-Treated Rats Is Associated with Induction of Inducible Nitric Oxide Synthase (iNOS), Androgen Receptor Suppression and Germ Cell Apoptosis. Int J Mol Sci 2017 May 15 [PMID: 28505146] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Panayotopoulou EG, Muller AK, Borries M et al. Targeting of apoptotic pathways by SMAC or BH3 mimetics distinctly sensitizes paclitaxel-resistant triple negative breast cancer cells Oncotarget 2017 Jul 11 [PMID: 28187446] (Human) Human
Lee EB, Porta S, Michael Baer G et al. Expansion of the classification of FTLD-TDP: distinct pathology associated with rapidly progressive frontotemporal degeneration. Acta Neuropathol 2017 Jan 27 [PMID: 28130640]
Redmann M, Wani WY, Volpicelli-Daley L et al. Trehalose does not improve neuronal survival on exposure to alpha-synuclein pre-formed fibrils. Redox Biol 2017 Jan 3 [PMID: 28068606]
Show All 146 Publications.

Reviews for p62/SQSTM1 Antibody (H00008878-M01) (6) 4.76

Average Rating: 4.7
(Based on 6 reviews)
We have 6 reviews tested in 3 species: Human, Mouse, Rat.
We have 6 reviews tested in 3 application: WB, IF, IP.

Reviews using H00008878-M01:
Filter by Applications
WB
(4)
IF
(1)
IP
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(3)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Woojong Lee
WB Mouse 05/04/2017
View

Summary

ApplicationWestern Blot
Sample TestedDEndritic cell lysate
SpeciesMouse
Immunofluorescence p62/SQSTM1 H00008878-M01
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reviewed by:
Anonymous
IF Human 04/05/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedDifferentiated cell line
SpeciesHuman

Blocking

Blocking DetailsBlocking with 2%FBS for 30min

Primary Anitbody

Dilution Ratio1:200 in PBS 1x

Secondary Antibody

Secondary DescriptionAlexa Fluor 546 donkey-anti-mouse
Secondary Concentration1:1000

Details

Detection NotesFluorescence Microscope
Fixation DetailsFixation in 4% PFA for 20min
Western Blot p62/SQSTM1 H00008878-M01
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reviewed by:
Fabrice GANKAM
WB Rat 11/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
CommentsExcellent antibody. detect 2 bands though at the right MW. too bad I cut me membrane :-)

Blocking

Blocking DetailsMilk 6% in PBST0.02% for 1hr RT

Primary Anitbody

Dilution Ratio1/5000 for 8 hrs at 4degrees

Secondary Antibody

Secondary Descriptionanti rabbit HRP
Secondary Manufacturer Cat#Pierce thermoscientific
Secondary Concentration1/10000

Details

Detection NotesECL (biorad clarity) with biorad chemidoc exposure time 10 sec

Comments

CommentsExcellent antibody. detect 2 bands though at the right MW. too bad I cut me membrane :-)
Western Blot p62/SQSTM1 H00008878-M01
Enlarge
reviewed by:
Anonymous
WB Mouse 10/24/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesMouse

Blocking

Blocking Details6% Non-fat milk in TBS at 4C for overnight

Primary Anitbody

Dilution Ratio1/1000 dilution in 3% non-fat milk in TBST (0.1% tween) at room temp for 1hr

Secondary Antibody

Secondary DescriptionRabbit Anti-Mouse HRP (IgG H&L)
Secondary Manufacturer Cat#biorad #172-1011
Secondary Concentration1:10000 dilution

Details

Detection Noteslane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells
Immunoprecipitation p62/SQSTM1 H00008878-M01
Enlarge
reviewed by:
Nadine Bakkar
IP Human 08/07/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedHuman spinal cord cytoplasmic homogenates
SpeciesHuman
CommentsInteraction between KEAP and p62/sqstm is previously reported.

Blocking

Blocking DetailsLIcoR blocking buffer

Primary Anitbody

Dilution RatioRb KEAP from PRoteintech

Secondary Antibody

Secondary Descriptionanti-rabbit secondary antibody from LicoR, IRdye 800
Secondary Manufacturer Cat#LicoR
Secondary Concentration1:20000

Details

Detection NotesLicoR Odyssey
Immunoprecipitation DetailsProtein A/G agarose, Overnight incubation in 1%NP-40, Washes in RIPA
Elution Description4xSDS dye, 5min boiling.

Comments

CommentsInteraction between KEAP and p62/sqstm is previously reported.
 
reviewed by:
Lin Zhang
WB Mouse 09/28/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p62/SQSTM1 Antibody (H00008878-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for p62/SQSTM1 Antibody (H00008878-M01)

Discover related pathways, diseases and genes to p62/SQSTM1 Antibody (H00008878-M01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on p62/SQSTM1.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

Required proteins for p62/SQSTM1 regulation and a role for p62/SQSTM1 in neuronal autophagy
Autophagy is a crucial cellular process that clears the cell of protein aggregates, toxins, and damaged cell products. Accumulation of toxins, damaged cell products and unwanted proteins has been proven to play a role in aging and many forms of dis...  Read full blog post.

ATG4C - A regulator of the early steps of autophagosome assembly
Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...  Read full blog post.

p62/SQSTM1 - targeting ubiquitinated proteins for autophagic degradation
During autophagy ubiquitinated cargo or substrates are engulfed in a double-membrane autophagosome and transported to the lysosome for degradation. This process is important for maintaining cellular homeostasis and for degrading damaged organelles...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

p62/SQSTM1 (sequestosome 1)
p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adaptor protein. SQSTM1 functions as a signaling hub for various signal transduction pathways involved in apoptosis, cell differentiation, apoptosis, immune response, and K+ channel reg...  Read full blog post.

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Recent Reviews

4.7
5
5
4
0
3
1
2
0
1
0

 
Woojong Lee
05/04/2017
Application: WB
Species: Mouse

Anonymous
04/05/2016
Application: IF
Species: Human

Fabrice GANKAM
11/01/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol SQSTM1
Entrez
Uniprot