p62/SQSTM1 Antibody (2C11)

Images

 
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).
Genetic Strategies: Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of p62 in p62 WT and KO mouse hepatocytes and HepG2 cell lysate using anti-p62/SQSTM1 antibody clone 2C11. Sample lane1: ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - ICC-IF analysis of SQSTM1 protein in HeLa cell using SQSTM1/p62 antibody (clone 2C11) at 10 ug/ml concentration.
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62 in SH-SY5Y cells using anti-p62/SQSTM1 antibody. Red - p62 puncta; Blue - nuclear DAPI; Green - Cytoskeleton. Image ...read more
ELISA: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Detection limit for recombinant GST tagged SQSTM1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
Clone
2C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

p62/SQSTM1 Antibody (2C11) Summary

Immunogen
SQSTM1 (AAH03139.1, 1 a.a. - 440 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Localization
Cytoplasmic
Specificity
SQSTM1 - sequestosome 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SQSTM1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Control
p62/SQSTM1 Lysate (NBP2-64781)
p62/SQSTM1 Lysate (NBP2-65906)
Reviewed Applications
Read 7 Reviews rated 4.6
using
H00008878-M01 in the following applications:

Publications
Read Publications using
H00008878-M01 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24352657). Human reactivity reported in scientific literature (PMID: 26571504). Rabbit reactivity reported in scientific literature (PMID: 23569437). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p62/SQSTM1 Antibody (2C11)

  • A170
  • EBI3-associated protein of 60 kDa
  • EBI3-associated protein p60
  • EBIAP
  • ORCA
  • OSIL
  • oxidative stress induced like
  • p60PDB3
  • p62
  • p62B
  • Paget disease of bone 3
  • PDB3
  • phosphotyrosine independent ligand for the Lck SH2 domain p62
  • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
  • Sequestosome 1
  • sequestosome-1
  • SQSTM1
  • Ubiquitin-binding protein p62
  • ZIP3

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in this gene result in sporadic and familial Paget disease of bone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IP

Publications for p62/SQSTM1 Antibody (H00008878-M01)(174)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(7)
IHC
(4)
IHC-Fr
(2)
IHC-P
(1)
IP
(1)
WB
(51)
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Filter By Species
Human
(27)
Mouse
(28)
Rat
(5)
Rabbit
(1)
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Showing Publications 1 - 10 of 174. Show All 174 Publications.
Publications using H00008878-M01 Applications Species
Zhang Y, Mun SR, Linares JF et al. ZZ-dependent regulation of p62/SQSTM1 in autophagy. Nat Commun. Oct 22 2018 [PMID: 30349045] (WB, Human) WB Human
Ashraf N, Duarte-Silva S, Shaw E et al. Citalopram Reduces Aggregation of ATXN3 in a YAC Transgenic Mouse Model of Machado-Joseph Disease. Mol. Neurobiol. Sep 4 2018 [PMID: 30187384] (WB, Mouse) WB Mouse
Palomo GM, Granatiero V, Kawamata H et al. Parkin is a disease modifier in the mutant SOD1 mouse model of ALS EMBO Mol Med Aug 20 2018 [PMID: 30126943] (WB, IHC, Mouse) WB, IHC Mouse
Schweitzer GG, Collier SL, Chen Z et al. Loss of lipin 1-mediated phosphatidic acid phosphohydrolase activity in muscle leads to skeletal myopathy in mice. FASEB J. Jul 20 2018 [PMID: 30028636] (IHC-Fr, Mouse) IHC-Fr Mouse
Henderson MX, Peng C, Trojanowski JQ, Lee VMY. LRRK2 activity does not dramatically alter alpha-synuclein pathology in primary neurons. Acta Neuropathol Commun 2018 May 31 [PMID: 29855356] (Human) Human
Khan W, Layden BT, Chakrabarti P. Inhibition of mTOR complexes protects cancer cells from glutamine starvation induced cell death by restoring Akt stability. Biochim. Biophys. Acta. Mar 16 2018 [PMID: 29555211] (WB) WB
Bresciani A, Spiezia MC, Boggio R et al. Quantifying autophagy using novel LC3B and p62 TR-FRET assays. PLoS One 2018 Mar 19 [PMID: 29554128]
Thomas-Jardin SE, Kanchwala MS, Jacob J et al. Identification of an IL-1-induced gene expression pattern in AR(+) PCa cells that mimics the molecular phenotype of AR(-) PCa cells. Prostate 2018 Jun [PMID: 29527701]
Ordonez-Gutierrez L, Benito-Cuesta I, Abad JL, Casas J. Dihydroceramide Desaturase 1 Inhibitors Reduce Amyloid-b Levels in Primary Neurons from an Alzheimer's Disease Transgenic Model. Pharm. Res. 2018 Feb 06 [PMID: 29411122] (Mouse) Mouse
Koukourakis MI, Giatromanolaki A, Fylaktakidou K, Sivridis E. SMER28 is a mTOR-independent small molecule enhancer of autophagy that protects mouse bone morrow and liver against radiotherapy. Invest New Drugs. 2018 Jan 31 [PMID: 29387992] (WB, Mouse) WB Mouse
Show All 174 Publications.

Reviews for p62/SQSTM1 Antibody (H00008878-M01) (7) 4.67

Average Rating: 4.6
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using H00008878-M01:
Filter by Applications
WB
(5)
IF
(1)
IP
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(4)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot p62/SQSTM1 H00008878-M01
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reviewed by:
Anonymous
WB Mouse 12/18/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse embryonic fibroblasts
SpeciesMouse
LotH5191-2C11

Comments

Commentsp62 knockout mouse embryonic fibroblasts
Name: Anti-p62 antibody (H00008878-M01)
Catalog #: Anti-p62 antibody (H00008878-M01)
Lot Number: Anti-p62 antibody (H00008878-M01, Lot # H5191-2C11)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): lane 1: WT mouse embryonic fibroblasts; lane 2: p62 knockout mouse embryonic fibroblasts
Sample Information:
     Cell Line or Tissue: mouse embryonic fibroblasts;
     Species: mouse
     Treatment: No treatment
Lysate Preparation:
     Date of lysate preparation: December 15, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: Wild type
     Negative Control: Knockout
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 8%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/1000
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times

Secondary Antibody
Manufacturer and Catalog #: Promega, W402B, Lot # 0000191071
Secondary description: goat anti-mouse secondary antibody
     Dilution: 1/2000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot # 9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 20 seconds
Molecular weight of band(s): ~ 65 kDa
Experimental Concerns and Observations: A specific band around 65 kDa was observed
 
reviewed by:
Woojong Lee
WB Mouse 05/04/2017
View

Summary

ApplicationWestern Blot
Sample TestedDEndritic cell lysate
SpeciesMouse
Immunofluorescence p62/SQSTM1 H00008878-M01
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reviewed by:
Anonymous
IF Human 04/05/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedDifferentiated cell line
SpeciesHuman

Blocking

Blocking DetailsBlocking with 2%FBS for 30min

Primary Anitbody

Dilution Ratio1:200 in PBS 1x

Secondary Antibody

Secondary DescriptionAlexa Fluor 546 donkey-anti-mouse
Secondary Concentration1:1000

Details

Detection NotesFluorescence Microscope
Fixation DetailsFixation in 4% PFA for 20min
Western Blot p62/SQSTM1 H00008878-M01
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reviewed by:
Fabrice GANKAM
WB Rat 11/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
CommentsExcellent antibody. detect 2 bands though at the right MW. too bad I cut me membrane :-)

Blocking

Blocking DetailsMilk 6% in PBST0.02% for 1hr RT

Primary Anitbody

Dilution Ratio1/5000 for 8 hrs at 4degrees

Secondary Antibody

Secondary Descriptionanti rabbit HRP
Secondary Manufacturer Cat#Pierce thermoscientific
Secondary Concentration1/10000

Details

Detection NotesECL (biorad clarity) with biorad chemidoc exposure time 10 sec

Comments

CommentsExcellent antibody. detect 2 bands though at the right MW. too bad I cut me membrane :-)
Western Blot p62/SQSTM1 H00008878-M01
Enlarge
reviewed by:
Anonymous
WB Mouse 10/24/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesMouse

Blocking

Blocking Details6% Non-fat milk in TBS at 4C for overnight

Primary Anitbody

Dilution Ratio1/1000 dilution in 3% non-fat milk in TBST (0.1% tween) at room temp for 1hr

Secondary Antibody

Secondary DescriptionRabbit Anti-Mouse HRP (IgG H&L)
Secondary Manufacturer Cat#biorad #172-1011
Secondary Concentration1:10000 dilution

Details

Detection Noteslane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells
Immunoprecipitation p62/SQSTM1 H00008878-M01
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reviewed by:
Nadine Bakkar
IP Human 08/07/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedHuman spinal cord cytoplasmic homogenates
SpeciesHuman
CommentsInteraction between KEAP and p62/sqstm is previously reported.

Blocking

Blocking DetailsLIcoR blocking buffer

Primary Anitbody

Dilution RatioRb KEAP from PRoteintech

Secondary Antibody

Secondary Descriptionanti-rabbit secondary antibody from LicoR, IRdye 800
Secondary Manufacturer Cat#LicoR
Secondary Concentration1:20000

Details

Detection NotesLicoR Odyssey
Immunoprecipitation DetailsProtein A/G agarose, Overnight incubation in 1%NP-40, Washes in RIPA
Elution Description4xSDS dye, 5min boiling.

Comments

CommentsInteraction between KEAP and p62/sqstm is previously reported.
 
reviewed by:
Lin Zhang
WB Mouse 09/28/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Product General Protocols

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ICC/IF Video Protocol

FAQs for p62/SQSTM1 Antibody (H00008878-M01) (0)

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Blogs on p62/SQSTM1. Showing 1-10 of 15 blog posts - Show all blog posts.

How a cell "reaches" out for help
By Christina Towers, PhD. Parkinson's disease is a debilitating neurodegenerative condition defined by the accumulation of alpha-synuclein-containing (alpha-SYN) intra-cytoplasmic inclusions, called Lewy bodies. The...  Read full blog post.

Measuring Autophagic Flux with LC3 protein levels: The do's and don'ts
By Christina Towers, PhD. Autophagy is a dynamic cellular recycling process that can be influenced by many different external and internal stimuli. The most commonly used assay to measure autophagy is a western blot f...  Read full blog post.

Crosstalk Between Oxidative Stress and Autophagy
By Christina Towers, PhD. Role of Reactive Species in Cellular FunctionOxidative stress is a byproduct of an imbalance between oxidants and antioxidants present in the cell resulting in dysfunctional redox signali...  Read full blog post.

Lysosomal Dysfunction is Linked to Exosomal Secretion
By Christina Towers, PhD. Lysosomal Dysfunction and DiseaseLysosomes are highly acidic organelles that are critical for cellular function and indispensable for degradative pathways like autophagy and endocytosis....  Read full blog post.

Nuclear LC3: Why is it there and what is it doing?
By Christina Towers, PhD. Cells use the complex process of autophagy to degrade and recycle cytoplasmic material.  There are over 20 proteins that have been implicated in this process and appropriately named core...  Read full blog post.

Autophagy inhibition in pediatrics: One physician-scientist’s brave decision
By Christina Towers, PhD. The current time from when a discovery is first made on the bench to when that discovery might translate into an approved therapy in cancer patients is an astounding 10-15 years. Scientists...  Read full blog post.

Cleaner gone bad: Autophagy regulates motor neuron loss in spinal muscular atrophy
By Jamshed Arslan Pharm.D. Neuromuscular disorders affect the peripheral nervous system and muscles. Spinal muscular atrophy (SMA) is one such incurable disease in which muscles fail to receive signals from the spi...  Read full blog post.

CaMKII stimulates autophagic degradation of 'ID', a new frontier against cancer
By Yoskaly Lazo-Fernandez, PhD The field of Cancer Stem Cell (CSC) research has been gaining traction in recent years1. CSCs are a minority group of cells (usually about 1 in 10000) within solid tumors of hematolog...  Read full blog post.

Brain size matters: MTOR regulates autophagy and number of cortical interneurons
By Jamshed Arslan Pharm.D. Interneurons transmit impulses between other neurons, in part, to facilitate the birth of neurons. Cortical interneurons themselves arise from the progenitors in the ventral telencephalon,...  Read full blog post.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.

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Recent Reviews

4.6
5
5
4
1
3
1
2
0
1
0

Anonymous
12/18/2017
Application: WB
Species: Mouse

 
Woojong Lee
05/04/2017
Application: WB
Species: Mouse

Anonymous
04/05/2016
Application: IF
Species: Human

Bioinformatics

Gene Symbol SQSTM1
Entrez
Uniprot