p62/SQSTM1 Antibody (2C11)

Images

 
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).
Genetic Strategies: Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of p62 in p62 WT and KO mouse hepatocytes and HepG2 cell lysate using anti-p62/SQSTM1 antibody clone 2C11. Sample lane1: ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - ICC-IF analysis of SQSTM1 protein in HeLa cell using SQSTM1/p62 antibody (clone 2C11) at 10 ug/ml concentration.
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62 in SH-SY5Y cells using anti-p62/SQSTM1 antibody. Red - p62 puncta; Blue - nuclear DAPI; Green - Cytoskeleton. Image ...read more
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Disruption of the VANGL2-p62/SQSTM1 interaction in breast cancer cells. Soft agar colony formation of T47D cells overexpressing GFP, GFP-VANGL2 and ...read more
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Confirmation of loss of hepatic ATG7 protein and a phenotype of hepatic autophagy inactivation. Fourth ug of liver protein were loaded on 4-20% gradient ...read more
ELISA: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Detection limit for recombinant GST tagged SQSTM1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ha, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, S-ELISA
Clone
2C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

p62/SQSTM1 Antibody (2C11) Summary

Immunogen
SQSTM1 (AAH03139.1, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Localization
Cytoplasmic
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SQSTM1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA
  • Immunocytochemistry/Immunofluorescence 10ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:1000
  • Sandwich ELISA
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 23569437).
Theoretical MW
47.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
p62/SQSTM1 Knockout HeLa Cell Lysate
p62/SQSTM1 Knockout 293T Cell Lysate
Reviewed Applications
Read 8 Reviews rated 4.5
using
H00008878-M01 in the following applications:

Publications
Read Publications using
H00008878-M01 in the following applications:

  • IB
    1 publication
  • 11 publications
  • IHC
    4 publications
  • 2 publications
  • 2 publications
  • WB
    62 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24352657). Human reactivity reported in scientific literature (PMID: 26571504). Rabbit reactivity reported in scientific literature (PMID: 23569437). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p62/SQSTM1 Antibody (2C11)

  • A170
  • Autophagy Receptor P62
  • DMRV
  • EBI3-associated protein of 60 kDa
  • EBI3-associated protein p60
  • EBIAP
  • FTDALS3
  • NADGP
  • ORCA
  • OSIL
  • oxidative stress induced like
  • p60PDB3
  • p62
  • p62B
  • Paget disease of bone 3
  • PDB3
  • phosphotyrosine independent ligand for the Lck SH2 domain p62
  • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
  • Sequestosome 1
  • sequestosome-1
  • SQSTM1
  • Ubiquitin-binding protein p62
  • ZIP3

Background

p62/SQSTM1 (ubiquitin-binding protein p62/Sequestrosome-1) is an intracellular protein (theoretical molecular weight 47.7 kDa) which localizes to the cytoplasm, nucleus, autophagosomes and lysosomes in all tissues (1). Structurally, p62/SQSTM1 consists of multiple domains (e.g., Phox1 and Bem1p-PB1, zinc finger-ZZ, TRAF6 binding domain-TB, Keap1-interacting region-KIR, LC3 interacting region-LIR and ubiquitin-associated domain-UBA) for interaction with various protein targets. Additional domains include nuclear export (NES) and nuclear localization signals (NLS1 and NLS2). p62/SQSTM1 is a multifunctional scaffold protein which forms oligomers with itself or with other proteins through interactions with PB1 domains.

Abnormal function of p62/SQSTM1 is associated with a range of disease states such as neurodegeneration, cancer, and metabolic disorders (2). Mutations in the p62/SQSTM1 sequence have been linked to Paget's disease of the bone, amyotrophic lateral sclerosis, and frontotemporal lobar degeneration. In Parkinson's disease, p62/SQSTM1 has been linked to microglia activation and subsequent neuroinflammation (3). Functionally, p62/SQSTM1 is involved in a broad range of cellular processes such as amino acid sensing by interaction with mTORC1, oxidative stress response through interaction with Keap1, and targeting cargo for autophagy by interacting with ubiquitin labeled proteins (1).

To induce selective autophagy, p62/SQSTM1 forms long oligomers or helical filaments which interact with LC3 and ubiquitin labeled proteins and lead to the initiation of the autophagosome formation (2). p62/SQSTM1 is not only a selective autophagy receptor but also an autophagy substrate, as its engulfed by the autophagosome and degraded by the autophagolysosome. Monitoring LC3 levels is the standard for assessing autophagic flux, however monitoring p62/SQSTM1 levels by Western blot in the presence and absence of autophagy inhibitors (e.g., Chloroquine) is also a common practice (4). Besides its activity as a selective autophagy receptor, p62/SQSTM1 also plays a role as an adaptor in signaling cascades leading to NFkB activation downstream of TNF-R, IL-1 beta R, TrkA and p75NTR. Briefly, for NFkB signaling downstream of the TNF-R activation, p62/SQSTM1 engages RIP1 kinase and PCK lambda/iota through the ZZ and PB1 domains, respectively (5).

References

1.Katsuragi, Y., Ichimura, Y., & Komatsu, M. (2015). P62/SQSTM1 functions as a signaling hub and an autophagy adaptor. FEBS Journal. https://doi.org/10.1111/febs.13540

2. Sanchez-Martin, P., & Komatsu, M. (2018). p62/SQSTM1 - Steering the cell through health and disease. Journal of Cell Science. https://doi.org/10.1242/jcs.222836

3. Yao, L., Zhu, Z., Wu, J., Zhang, Y., Zhang, H., Sun, X.,... Lu, G. (2019). MicroRNA-124 regulates the expression of p62/p38 and promotes autophagy in the inflammatory pathogenesis of Parkinson's disease. The FASEB Journal. https://doi.org/10.1096/fj.201900363r

4 Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A.,... Zughaier, S. M. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. https://doi.org/10.1080/15548627.2015.1100356

5. Bitto, A., Lerner, C. A., Nacarelli, T., Crowe, E., Torres, C., & Sell, C. (2014). p62/SQSTM1 at the interface of aging, autophagy, and disease. Age. https://doi.org/10.1007/s11357-014-9626-3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2331
Species: Hu, Mu, Rt, Ca, Fi, Pl, Ze
Applications: WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SB, IHC-WhMt
NBP1-32822
Species: Hu, Mu, Rt, Al, Av, Bv, Ce, Pl
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
NB100-56618
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-CS
NB100-56583
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
NB500-249
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
H00002965-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, RNAi, S-ELISA
NBP1-82454
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-607
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P
NB200-106
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56508
Species: Hu, Mu, Rt, Av
Applications: WB, Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
NB100-82112
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-15939
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF269
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
NBP1-81404
Species: Hu
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP3-04662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IP

Publications for p62/SQSTM1 Antibody (H00008878-M01)(245)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IB
(1)
ICC/IF
(11)
IHC
(4)
IHC-Fr
(2)
IHC-P
(2)
WB
(62)
All Applications
Filter By Species
Human
(37)
Mouse
(35)
Rat
(5)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 245. Show All 245 Publications.
Publications using H00008878-M01 Applications Species
Abokyi S, Shan S, To C et al. Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells Oxidative Medicine and Cellular Longevity Jul 22 2020 [PMID: 32774677] (WB, Human) WB Human
An H, Ordureau A, Korner M et al. Systematic quantitative analysis of ribosome inventory during nutrient stress Nature Jul 1 2020 [PMID: 32612236]
Dulac M, Leduc-Gaudet JP, Reynaud O et al. Drp1 knockdown induces severe muscle atrophy and remodelling, mitochondrial dysfunction, autophagy impairment and denervation J. Physiol. (Lond.) Jun 15 2020 [PMID: 32539155]
Hossain MI, Marcus JM, Lee JH et al. Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective Autophagy May 25 2020 [PMID: 32450052] (ICC/IF, Mouse) ICC/IF Mouse
Van Dyck L, Vanhorebeek I, Wilmer A et al. Towards a fasting-mimicking diet for critically ill patients: the pilot randomized crossover ICU-FM-1 study Crit Care May 24 2020 [PMID: 32448392] (WB, Human) WB Human
Liao K, Niu F, Hu G et al. HIV Tat-mediated induction of autophagy regulates the disruption of ZO-1 in brain endothelial cells Tissue Barriers Apr 16 2020 [PMID: 32299282]
Towers Cg, Wodetzki D, Thorburn A Autophagy-dependent cancer cells circumvent loss of the upstream regulator RB1CC1/FIP200 and loss of LC3 conjugation by similar mechanisms Autophagy Mar 18 2020 [PMID: 32160093] (WB, Human) WB Human
Benito-Cuesta, I., Ordonez-Gutierrez, L., Wandosell, F. AMPK activation does not enhance autophagy in neurons in contrast to MTORC1 inhibition: different impact on beta-amyloid clearance Autophagy Feb 20 2020 [PMID: 32075509] (WB, Human, Mouse) WB Human, Mouse
Pires Da Silva J, Monceaux K, Guilbert A et al. SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy Cells Feb 12 2020 [PMID: 32059483] (WB, Mouse) WB Mouse
Li P, Shi M, Maique J et al. Beclin 1/Bcl2 complex-dependent autophagy activity modulates renal susceptibility to ischemia-reperfusion injury and mediates renoprotection by Klotho Am. J. Physiol. Renal Physiol. Jan 27 2020 [PMID: 31984794]
Show All 245 Publications.

Reviews for p62/SQSTM1 Antibody (H00008878-M01) (8) 4.58

Average Rating: 4.5
(Based on 8 reviews)
We have 8 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using H00008878-M01:
Filter by Applications
WB
(6)
IF
(1)
IP
(1)
All Applications
Filter by Species
Human
(3)
Mouse
(4)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot p62/SQSTM1 H00008878-M01
Enlarge
4
reviewed by:
Anonymous
WB Human 08/17/2019
View

Summary

ApplicationWestern Blot
Sample TestedHEK293
SpeciesHuman
LotH5191-2C11

Comments

Comments60ug of total cell lysate was loaded for the KO screening. There were several non-specific bands below 50Kda region.
Western Blot p62/SQSTM1 H00008878-M01
Enlarge
4
reviewed by:
Anonymous
WB Mouse 12/18/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse embryonic fibroblasts
SpeciesMouse
LotH5191-2C11

Comments

Commentsp62 knockout mouse embryonic fibroblasts
Name: Anti-p62 antibody (H00008878-M01)
Catalog #: Anti-p62 antibody (H00008878-M01)
Lot Number: Anti-p62 antibody (H00008878-M01, Lot # H5191-2C11)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): lane 1: WT mouse embryonic fibroblasts; lane 2: p62 knockout mouse embryonic fibroblasts
Sample Information:
     Cell Line or Tissue: mouse embryonic fibroblasts;
     Species: mouse
     Treatment: No treatment
Lysate Preparation:
     Date of lysate preparation: December 15, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: Wild type
     Negative Control: Knockout
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 8%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/1000
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times

Secondary Antibody
Manufacturer and Catalog #: Promega, W402B, Lot # 0000191071
Secondary description: goat anti-mouse secondary antibody
     Dilution: 1/2000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot # 9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 20 seconds
Molecular weight of band(s): ~ 65 kDa
Experimental Concerns and Observations: A specific band around 65 kDa was observed
  5
reviewed by:
Woojong Lee
WB Mouse 05/04/2017
View

Summary

ApplicationWestern Blot
Sample TestedDEndritic cell lysate
SpeciesMouse
Immunofluorescence p62/SQSTM1 H00008878-M01
Enlarge
5
reviewed by:
Anonymous
IF Human 04/05/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedDifferentiated cell line
SpeciesHuman

Blocking

Blocking DetailsBlocking with 2%FBS for 30min

Primary Anitbody

Dilution Ratio1:200 in PBS 1x

Secondary Antibody

Secondary DescriptionAlexa Fluor 546 donkey-anti-mouse
Secondary Concentration1:1000

Details

Detection NotesFluorescence Microscope
Fixation DetailsFixation in 4% PFA for 20min
Western Blot p62/SQSTM1 H00008878-M01
Enlarge
5
reviewed by:
Fabrice GANKAM
WB Rat 11/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
CommentsExcellent antibody. detect 2 bands though at the right MW. too bad I cut me membrane :-)

Blocking

Blocking DetailsMilk 6% in PBST0.02% for 1hr RT

Primary Anitbody

Dilution Ratio1/5000 for 8 hrs at 4degrees

Secondary Antibody

Secondary Descriptionanti rabbit HRP
Secondary Manufacturer Cat#Pierce thermoscientific
Secondary Concentration1/10000

Details

Detection NotesECL (biorad clarity) with biorad chemidoc exposure time 10 sec

Comments

CommentsExcellent antibody. detect 2 bands though at the right MW. too bad I cut me membrane :-)
Western Blot p62/SQSTM1 H00008878-M01
Enlarge
5
reviewed by:
Anonymous
WB Mouse 10/24/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesMouse

Blocking

Blocking Details6% Non-fat milk in TBS at 4C for overnight

Primary Anitbody

Dilution Ratio1/1000 dilution in 3% non-fat milk in TBST (0.1% tween) at room temp for 1hr

Secondary Antibody

Secondary DescriptionRabbit Anti-Mouse HRP (IgG H&L)
Secondary Manufacturer Cat#biorad #172-1011
Secondary Concentration1:10000 dilution

Details

Detection Noteslane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells
Immunoprecipitation p62/SQSTM1 H00008878-M01
Enlarge
5
reviewed by:
Nadine Bakkar
IP Human 08/07/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedHuman spinal cord cytoplasmic homogenates
SpeciesHuman
CommentsInteraction between KEAP and p62/sqstm is previously reported.

Blocking

Blocking DetailsLIcoR blocking buffer

Primary Anitbody

Dilution RatioRb KEAP from PRoteintech

Secondary Antibody

Secondary Descriptionanti-rabbit secondary antibody from LicoR, IRdye 800
Secondary Manufacturer Cat#LicoR
Secondary Concentration1:20000

Details

Detection NotesLicoR Odyssey
Immunoprecipitation DetailsProtein A/G agarose, Overnight incubation in 1%NP-40, Washes in RIPA
Elution Description4xSDS dye, 5min boiling.

Comments

CommentsInteraction between KEAP and p62/sqstm is previously reported.
  3
reviewed by:
Lin Zhang
WB Mouse 09/28/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p62/SQSTM1 Antibody (H00008878-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional p62/SQSTM1 Products

Bioinformatics Tool for p62/SQSTM1 Antibody (H00008878-M01)

Discover related pathways, diseases and genes to p62/SQSTM1 Antibody (H00008878-M01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for p62/SQSTM1 Antibody (H00008878-M01)

Discover more about diseases related to p62/SQSTM1 Antibody (H00008878-M01).
 

Pathways for p62/SQSTM1 Antibody (H00008878-M01)

View related products by pathway.

PTMs for p62/SQSTM1 Antibody (H00008878-M01)

Learn more about PTMs related to p62/SQSTM1 Antibody (H00008878-M01).
 

Research Areas for p62/SQSTM1 Antibody (H00008878-M01)

Find related products by research area.

Blogs on p62/SQSTM1. Showing 1-10 of 23 blog posts - Show all blog posts.

Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles
By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox...  Read full blog post.

Autophagy Research Update: What a difference a year makes!
By Christina Towers, PhD Over the last two decades the field of autophagy has exploded! Innovative techniques, comprehensive analysis and disease-relevant models have yielded basic and clinical discoveries of conseque...  Read full blog post.

Autophagy and Metastasis
  By Christina Towers, PhD The majority of cancer patients die from metastatic disease at secondary sites. The threshold to undergo metastasis is high. Only a minority of cancer cells acquire invasive phenotypes...  Read full blog post.


  Read full blog post.


  Read full blog post.


  Read full blog post.

RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism
By Jamshed Arslan Pharm.D. Spinocerebellar ataxia type 2 (SCA2) is a movement disorder characterized by neurodegeneration. The cause of this autosomal dominant disease is a mutation in the RNA processing gene Atxn2,...  Read full blog post.

How a cell "reaches" out for help
By Christina Towers, PhD. Parkinson's disease is a debilitating neurodegenerative condition defined by the accumulation of alpha-synuclein-containing (alpha-SYN) intra-cytoplasmic inclusions, called Lewy bodies. The...  Read full blog post.

Measuring Autophagic Flux with LC3 protein levels: The do's and don'ts
By Christina Towers, PhD. Autophagy is a dynamic cellular recycling process that can be influenced by many different external and internal stimuli. The most commonly used assay to measure autophagy is a western blot f...  Read full blog post.


  Read full blog post.

Showing 1-10 of 23 blog posts - Show all blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.5
5
5
4
2
3
1
2
0
1
0

Anonymous
08/17/2019
Application: WB
Species: Human

Anonymous
12/18/2017
Application: WB
Species: Mouse

 
Woojong Lee
05/04/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SQSTM1
Entrez
Uniprot