SAM68 Antibody

Images

 
Western Blot: SAM68 Antibody [NBP1-19151] - Whole cell lysate from HeLa cells. SAM68 was also immunoprecipitated by rabbit anti-SAM68 antibodies NBP1-19152 and NBP1-19153.
Immunohistochemistry: SAM68 Antibody [NBP1-19151] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAM68 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MaSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

SAM68 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Src-associated in mitosis 68 kDa protein (KH domain containing, RNA binding, signal transduction associated 1) using the numbering given in entry NP_006550.1 (Gen
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KHDRBS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-19151 in the following applications:

Publications
Read Publications using
NBP1-19151 in the following applications:

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 27180817).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAM68 Antibody

  • FLJ34027
  • GAP-associated tyrosine phosphoprotein p62
  • KH domain containing, RNA binding, signal transduction associated 1
  • KH domain-containing, RNA-binding, signal transduction-associated protein 1
  • KHDRBS1
  • p21 Ras GTPase-activating protein-associated p62
  • p62
  • p68
  • SAM68
  • Sam68GAP-associated tyrosine phosphoprotein p62 (Sam68)
  • Src-associated in mitosis 68 kDa protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAM68 Antibody (NBP1-19151)(2)

We have publications tested in 3 confirmed species: Human, Bovine, Mammal.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(2)
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Human
(1)
Bovine
(1)
Mammal
(1)
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Review for SAM68 Antibody (NBP1-19151) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-19151:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SAM68 NBP1-19151
Enlarge
reviewed by:
Anonymous
WB Human 10/16/2015
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking DetailsBlocker=Skimmed milk, Buffer= 1XTris Buffer Saline+0.1 % Tween 20, 2hrs at RT

Primary Anitbody

Dilution Ratio1:500 over night at 4 degree Celsius

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#7074P2
Secondary Concentration1:20,000

Details

Detection Notes30 seconds exposure

Comments

CommentsIt gives two bands for SAM68, so be careful to choose one.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SAM68 Antibody (NBP1-19151) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SAM68 Antibody (NBP1-19151)

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Recent Reviews

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5
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4
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3
1
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0
1
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Anonymous
10/16/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol KHDRBS1
Entrez
Uniprot