Reactivity | Hu, MuSpecies Glossary |
Applications | IHC, ICC/IF |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | KLH-coupled human Caspase-3 synthetic peptide CRGTELDCGIETD Accession # P42574 |
Specificity | Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | CASP3 |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Images | Ratings | Applications | Species | Date | Details | ||||||||||||
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reviewed by:
Anonymous |
WB | Mouse | 01/09/2015 |
Summary
Blocking
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reviewed by:
Anonymous |
IHC-P | Mouse | 01/09/2015 |
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reviewed by:
Anonymous |
IHC-P | Mouse | 01/09/2015 |
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reviewed by:
Anonymous |
IHC-P | Other | 01/09/2015 |
Summary
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reviewed by:
Anonymous |
IF | Mouse | 01/09/2015 |
Summary
Blocking
Details
Comments
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reviewed by:
Anonymous |
IF | Mouse | 01/09/2015 |
Summary
Blocking
Details
Comments
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reviewed by:
Anonymous |
IHC-P | Mouse | 01/09/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for Caspase-3 Antibody (AF835)Discover more about diseases related to Caspase-3 Antibody (AF835).
| Pathways for Caspase-3 Antibody (AF835)View related products by pathway.
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PTMs for Caspase-3 Antibody (AF835)Learn more about PTMs related to Caspase-3 Antibody (AF835).
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The Ins and Outs of Survivin By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of... Read full blog post. |
Caspase-3- A marker of programmed cell death Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul... Read full blog post. |
Caspase 3, the executioner of apoptosis Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru... Read full blog post. |
Caspase 7 - A key effector of the apoptotic pathway Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a... Read full blog post. |
Caspase 11 - A proinflammatory caspase that induces the innate immune response While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in... Read full blog post. |
LC3/LC3B - measuring autophagosome formation and autophagic flux Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr... Read full blog post. |
D4-GDI (GDP dissociation inhibitor, RhoGD12) The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M... Read full blog post. |
LC3B - a novel marker for autophagosome Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a... Read full blog post. |
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Anonymous 01/09/2015 |
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Application: | WB | |
Species: | Mouse |
Anonymous 01/09/2015 |
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Application: | IHC-P | |
Species: | Mouse |
Anonymous 01/09/2015 |
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Application: | IHC-P | |
Species: | Mouse |