Caspase-3 Antibody [Unconjugated] - Active

Images

 
Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody ...read more
Caspase‑3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) ...read more
Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Caspase-3 Antibody [Unconjugated] - Active Summary

Immunogen
KLH-coupled human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # P42574
Specificity
Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
Reviewed Applications
Read 7 Reviews rated 4.9
using
AF835 in the following applications:

Publications
Read Publications using
AF835 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-3 Antibody [Unconjugated] - Active

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed, and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF835
Species: Hu, Mu
Applications: IHC, ICC/IF

Publications for Caspase-3 Antibody (AF835)(195)

We have publications tested in 15 confirmed species: Human, Mouse, Rat, Canine, Chicken, Feline, Fish, Fish - Cyprinus carpio (Carp), Guinea Pig, Lamb, Ovine, Porcine, Primate, Transgenic Mouse, Zebrafish.

We have publications tested in 11 applications: Flow Cytometry, Functional Assay, ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot, assay - see notes.


Filter By Application
Flow Cytometry
(4)
Functional Assay
(1)
ICC
(39)
ICC/IF
(1)
IF
(2)
IHC
(88)
IHC-Fr
(10)
IHC-P
(36)
Immunoprecipitation
(1)
Western Blot
(9)
assay - see notes
(1)
All Applications
Filter By Species
Human
(43)
Mouse
(114)
Rat
(10)
Canine
(1)
Chicken
(7)
Feline
(1)
Fish
(1)
Fish - Cyprinus carpio (Carp)
(1)
Guinea Pig
(1)
Lamb
(1)
Ovine
(3)
Porcine
(4)
Primate
(1)
Transgenic Mouse
(1)
Zebrafish
(4)
All Species
Showing Publications 1 - 10 of 195. Show All 195 Publications.
Publications using AF835 Applications Species
R Belgacemi, S Danopoulos, G Deutsch, I Glass, V Dormoy, S Bellusci, D Al Alam Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis International Journal of Molecular Sciences, 2022;23(9):. 2022 [PMID: 35563656] (IHC, Human) IHC Human
P Yamkate, JA Lidbury, JM Steiner, JS Suchodolsk, PR Giaretta Immunohistochemical Expression of Oxidative Stress and Apoptosis Markers in Archived Liver Specimens from Dogs with Chronic Hepatitis Journal of comparative pathology, 2022;193(0):25-36. 2022 [PMID: 35487620] (IHC, Canine) IHC Canine
H Wen, X Zhang, Q Li, J Huang, G Liu, J Zhao, Y Liu, L Shen, Y Li, K Yang, L Zuo, J Li, J Nian, P Xiang, H Zhao, L Yu, M Liu, Z Geng, X Song Ruscogenins Improve CD-Like Enteritis by Inhibiting Apoptosis of Intestinal Epithelial Cells and Activating Nrf2/NQO1 Pathway Oxidative Medicine and Cellular Longevity, 2022;2022(0):4877275. 2022 [PMID: 35308175] (ICC/IF, Mouse) ICC/IF Mouse
C Lee, J Kim, J Han, D Oh, M Kim, H Jeong, TJ Kim, SW Kim, JN Kim, YS Seo, A Suzuki, JH Kim, Y Jung Formyl peptide receptor 2 determines sex-specific differences in the progression of nonalcoholic fatty liver disease and steatohepatitis Nature Communications, 2022;13(1):578. 2022 [PMID: 35102146] (IHC, Mouse) IHC Mouse
S André, M Picard, R Cezar, F Roux-Dalva, A Alleaume-B, C Soundaramo, AS Cruz, A Mendes-Fri, C Gotti, M Leclercq, A Nicolas, A Tauzin, A Carvalho, C Capela, J Pedrosa, AG Castro, L Kundura, P Loubet, A Sotto, L Muller, JY Lefrant, C Roger, PG Claret, S Duvnjak, TA Tran, G Racine, O Zghidi-Abo, P Nioche, R Silvestre, A Droit, F Mammano, P Corbeau, J Estaquier T cell apoptosis characterizes severe Covid-19 disease Cell Death and Differentiation, 2022;0(0):. 2022 [PMID: 35066575] (ICC, Human) ICC Human
D Chen, ED Hughes, TL Saunders, J Wu, MN Hernández, T Makinen, PD King Angiogenesis depends upon EPHB4-mediated export of collagen IV from vascular endothelial cells JCI Insight, 2022;0(0):. 2022 [PMID: 35015735] (IHC, Mouse) IHC Mouse
J Schreiber, N Liaukouska, L Fuhrmann, AT Hauser, M Jung, TB Huber, N Wanner BET Proteins Regulate Expression of Osr1 in Early Kidney Development Biomedicines, 2021;9(12):. 2021 [PMID: 34944697] (ICC, Mouse) ICC Mouse
P Flora, MY Li, PM Galbo, M Astorkia, D Zheng, E Ezhkova Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration PloS Genetics, 2021;17(12):e1009948. 2021 [PMID: 34905545] (IHC, Transgenic Mouse) IHC Transgenic Mouse
B Schwarze, A Korn, C Höfling, U Zeitschel, M Krueger, S Ro beta ner, D Huster Peptide backbone modifications of amyloid beta (1-40) impact fibrillation behavior and neuronal toxicity Scientific Reports, 2021;11(1):23767. 2021 [PMID: 34887476] (ICC, Mouse) ICC Mouse
W Nakajima, S Nakanishi, R Hosoya, T Uemoto, S Ohgo, N Wada Regenerative Polarity of the Fin Ray in Zebrafish Caudal Fin and Related Tissue Formation on the Cut Surface Journal of developmental biology, 2021;9(4):. 2021 [PMID: 34842743] (IHC, Zebrafish) IHC Zebrafish
Show All 195 Publications.

Reviews for Caspase-3 Antibody (AF835) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Mouse, Other.

Reviews using AF835:
Filter by Applications
Western Blot
(1)
Immunohistochemistry-Paraffin
(4)
Immunofluorescence
(2)
All Applications
Filter by Species
Mouse
(6)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
Western Blot Mouse 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23374352
SpeciesMouse
  5
reviewed by:
Anonymous -
Immunohistochemistry-Paraffin Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20522641
SpeciesMouse
  5
reviewed by:
Anonymous -
Immunohistochemistry-Paraffin Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22897852
SpeciesMouse
  5
reviewed by:
Anonymous -
Immunohistochemistry-Paraffin Other 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22438018
SpeciesOther
  5
reviewed by:
Anonymous -
Immunofluorescence Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23532769
SpeciesMouse
  5
reviewed by:
Anonymous -
Immunofluorescence Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19226508
SpeciesMouse
  4
reviewed by:
Anonymous -
Immunohistochemistry-Paraffin Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23742999
SpeciesMouse

FAQs for Caspase-3 Antibody (AF835) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

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Blogs on Caspase-3. Showing 1-10 of 11 blog posts - Show all blog posts.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.


  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
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Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

 
Anonymous -
01/09/2015
Application: Western Blot
Species: Mouse

 
Anonymous -
01/09/2015
Application: Immunohistochemistry-Paraffin
Species: Mouse

 
Anonymous -
01/09/2015
Application: Immunohistochemistry-Paraffin
Species: Mouse

Bioinformatics

Gene Symbol CASP3
Uniprot