Caspase-3 Antibody [Unconjugated] - Active

Images

 
Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody ...read more
Caspase‑3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) ...read more
Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Caspase-3 Antibody [Unconjugated] - Active Summary

Immunogen
KLH-coupled human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # P42574
Specificity
Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 7 Reviews rated 4.9
using
AF835 in the following applications:

Publications
Read Publications using
AF835 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-3 Antibody [Unconjugated] - Active

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed, and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56098
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP
NB100-56118
Species: Hu, Mu, Rt, Ca, Ma
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-28566
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Func, IHC, IHC-Fr, IHC-P
NB100-56599
Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, Flow-IC
NB100-56116
Species: Hu, Mu, Rt, Ma
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56503
Species: Hu, Mu, Rt, Dr, Rb
Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, Flow-CS, Flow-IC
NB100-56104
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NBP2-43728
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
NB100-56183
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF835
Species: Hu, Mu
Applications: IHC, ICC/IF

Publications for Caspase-3 Antibody (AF835)(152)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Chicken, Feline, Fish, Fish - Cyprinus carpio (Carp), Guinea Pig, Lamb, Ovine, Porcine, Primate, Zebrafish.

We have publications tested in 8 applications: Flow Cytometry, Functional Assay, ICC, IF, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(4)
Functional Assay
(1)
ICC
(28)
IF
(1)
IHC
(62)
IHC-Fr
(10)
IHC-P
(36)
Western Blot
(7)
All Applications
Filter By Species
Human
(29)
Mouse
(92)
Rat
(7)
Chicken
(7)
Feline
(1)
Fish
(1)
Fish - Cyprinus carpio (Carp)
(1)
Guinea Pig
(1)
Lamb
(1)
Ovine
(3)
Porcine
(3)
Primate
(1)
Zebrafish
(3)
All Species
Showing Publications 1 - 10 of 152. Show All 152 Publications.
Publications using AF835 Applications Species
F Chang, SJ Kong, L Wang, BK Choi, H Lee, C Kim, JM Kim, K Park Targeting Actomyosin Contractility Suppresses Malignant Phenotypes of Acute Myeloid Leukemia Cells Int J Mol Sci, 2020;21(10):. 2020 [PMID: 32422910] (IHC, Mouse) IHC Mouse
J Aldaregia, P Errarte, A Olazagoiti, M Gimeno, JJ Uriz, TR Gershon, I Garcia, A Matheu Erbb4 Is Required for Cerebellar Developmentand Malignant Phenotype of Medulloblastoma Cancers (Basel), 2020;12(4):. 2020 [PMID: 32316671] (ICC, Mouse) ICC Mouse
NJ Yates, KW Feindel, A Mehnert, R Beare, S Quick, D Blache, JJ Pillow, RW Hunt Ex Vivo MRI Analytical Methods and Brain Pathology in Preterm Lambs Treated with Postnatal Dexamethasone � Brain Sci, 2020;10(4):. 2020 [PMID: 32260193] (IHC, Lamb) IHC Lamb
MA Khan, T Shamma, S Kazmi, A Altuhami, HA Ahmed, AM Assiri, DC Broering Hypoxia-induced complement dysregulation is associated with microvascular impairments in mouse tracheal transplants J Transl Med, 2020;18(1):147. 2020 [PMID: 32234039] (IHC, Mouse) IHC Mouse
P Aldaz, M Otaegi-Uga, A Saenz-Anto, M Garcia-Pug, M Moreno-Val, JM Flores, D Gerovska, MJ Arauzo-Bra, N Samprón, A Matheu, E Carrasco-G SOX9 promotes tumor progression through the axis BMI1-p21CIP Sci Rep, 2020;10(1):357. 2020 [PMID: 31941916] (IF, Human) IF Human
A Malhotra, M Castillo-M, BJ Allison, AE Sutherland, I Nitsos, Y Pham, CA McDonald, MC Fahey, GR Polglase, G Jenkin, SL Miller Neurovascular effects of umbilical cord blood-derived stem cells in growth-restricted newborn lambs : UCBCs for perinatal brain injury Stem Cell Res Ther, 2020;11(1):17. 2020 [PMID: 31915068]
K Boric, S Mardesic, D Martinovic, M Radic, I Tadin Hadj, K Vukojevic, I Kosovic, I Solic, S Zekic Toma, M Saraga-Bab Expression of apoptotic and proliferation factors in gastric mucosa of patients with systemic sclerosis correlates with form of the disease Sci Rep, 2019;9(1):18461. 2019 [PMID: 31804582] (IHC-P, Human) IHC-P Human
LG Jones, A Vaida, LM Thompson, FI Ikuomola, JH Caamaño, MD Burkitt, F Miyajima, JM Williams, BJ Campbell, DM Pritchard, CA Duckworth NF-kappaB2 signalling in enteroids modulates enterocyte responses to secreted factors from bone marrow-derived dendritic cells Cell Death Dis, 2019;10(12):896. 2019 [PMID: 31772152] (IHC, Mouse) IHC Mouse
E Grall, V Gourain, A Naïr, E Martin, MC Birling, JN Freund, I Duluc Severe head dysgenesis resulting from imbalance between anterior and posterior ontogenetic programs Cell Death Dis, 2019;10(11):812. 2019 [PMID: 31649239] (IHC-P, Mouse) IHC-P Mouse
D Aprile, F Fruscione, S Baldassari, M Fadda, D Ferrante, A Falace, E Buhler, J Sartorelli, A Represa, P Baldelli, F Benfenati, F Zara, A Fassio TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons Cell Death Differ., 2019;0(0):. 2019 [PMID: 30858606] (ICC, Rat) ICC Rat
Show All 152 Publications.

Reviews for Caspase-3 Antibody (AF835) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Mouse, Other.

Reviews using AF835:
Filter by Applications
WB
(1)
IHC-P
(4)
IF
(2)
All Applications
Filter by Species
Mouse
(6)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
WB Mouse 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23374352
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23374352

Details

Detection NotesSee PMID 23374352

Comments

CommentsPublished: See PMID 23374352Independent review from antibodyresource.com
  5
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20522641
SpeciesMouse
  5
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22897852
SpeciesMouse
  5
reviewed by:
Anonymous
IHC-P Other 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22438018
SpeciesOther
  5
reviewed by:
Anonymous
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23532769
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23532769

Details

Detection NotesSee PMID 23532769

Comments

CommentsPublished: See PMID 23532769Independent review from antibodyresource.com
  5
reviewed by:
Anonymous
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19226508
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19226508

Details

Detection NotesSee PMID 19226508

Comments

CommentsPublisheD: See PMID 19226508Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23742999
SpeciesMouse

FAQs for Caspase-3 Antibody (AF835) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Caspase-3 Products

Bioinformatics Tool for Caspase-3 Antibody (AF835)

Discover related pathways, diseases and genes to Caspase-3 Antibody (AF835). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Caspase-3 Antibody (AF835)

Discover more about diseases related to Caspase-3 Antibody (AF835).
 

Pathways for Caspase-3 Antibody (AF835)

View related products by pathway.

PTMs for Caspase-3 Antibody (AF835)

Learn more about PTMs related to Caspase-3 Antibody (AF835).

Blogs on Caspase-3.

Gut-brain axis: microbiota influence behavior and mental well-being
By Jennifer Sokolowski, MD, PhD.Gut-microbiome interactionsOn and in our bodies, microbes outnumber our cells by about ten-to-one. Studies have revealed that the microbiome influences neurogenesis, cognition, and...  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

 
Anonymous
01/09/2015
Application: WB
Species: Mouse

 
Anonymous
01/09/2015
Application: IHC-P
Species: Mouse

 
Anonymous
01/09/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol CASP3
Uniprot