Caspase-3 Antibody [Unconjugated] - Active

Images

 
Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody ...read more
Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) ...read more
Caspase‑3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
Immunohistochemical staining for caspase 3 on surgically transected tumours. A) Epo-treated tumour 24 hours after surgery. B) Placebo-treated tumour 24 hours after surgery. C) Placebo-treated tumour 48 hours after ...read more
LPS challenge induces cell shedding from small intestinal villi. C57 BL/6 mice were administered either (a) PBS (control) or (b) LPS by IP injection and proximal small intestines removed after 1.5 h. Processed tissue ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
The cytoprotective effect of B. breve is MyD88 dependent. (a,b) IEC MyD88+/+ mice and (c,d) IEC MyD88−/− mice were gavaged with PBS (control) or B. breve UCC2003 and challenged with LPS. Paraffin-embedded intestinal ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
Bifidobacterium breve UCC2003 protects against LPS-induced cell shedding. C57 BL/6 mice received three daily oral gavage doses of (a) PBS or (b) approximately 1 × 109B. breve UCC2003 followed by IP challenge with LPS ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
Genetic deletion of Bax prevents mutant LRRK2 induced neuronal death. Primary embryonic cortical neurons are derived from WT mice or mice lacking the pro-apoptotic protein Bax, and transiently transfected with ...read more
Hepatocyte ARNT deletion alters fibrotic and apoptotic gene expression in the TAA model of fibrosis.Results for FC are shown in black columns and LARNT in white columns. Results are expressed relative to FC level. (A) ...read more
p140Cap expression is causative in limiting tumour growth in NeuT mice.(a) Expression cassette used for the generation of MMTV-p140Cap transgenic mice. The Myc epitope is inserted at the carboxyterminal region of the ...read more
Microglia-derived TNF-alpha alleviates aberrant neurogenesis.(a) qRT-PCR analyses of Tnf-alpha levels in the DG of WT and TLR9 KO mice at the indicated time points after seizure. Experimental controls were ...read more
Double immunofluorescent staining of FSGS kidneys to Ki-67, alpha -tubulin and DAPI or double immunofluorescent staining to caspase-3 and DAPI. A. FSGS kidneys: cysts with simple squamous epithelium (CS) filled with ...read more
Epidermis-specific deletion of Rac1 increases UV-light-induced keratinocyte apoptosis in vivo. (a) H/E staining of UV-irradiated skin of Rac1 fl/fl and Rac1-EKO mice at 12 h after UV-irradiation. Black arrows indicate ...read more
Hepatocyte ARNT deletion alters fibrotic and apoptotic gene expression in the TAA model of fibrosis.Results for FC are shown in black columns and LARNT in white columns. Results are expressed relative to FC level. (A) ...read more
Immunohistochemical staining for caspase 3 on surgically transected tumours. A) Epo-treated tumour 24 hours after surgery. B) Placebo-treated tumour 24 hours after surgery. C) Placebo-treated tumour 48 hours after ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
DNA damage has an important role in increased UV-light-induced keratinocyte apoptosis in Rac1-EKO epidermis in vivo. (a and c) H/E stainings and immunostainings against cleaved caspase-3 (green) of UV-irradiated skin of ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
DNA damage has an important role in increased UV-light-induced keratinocyte apoptosis in Rac1-EKO epidermis in vivo. (a and c) H/E stainings and immunostainings against cleaved caspase-3 (green) of UV-irradiated skin of ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
Immunohistochemical staining for caspase 3 on surgically transected tumours. A) Epo-treated tumour 24 hours after surgery. B) Placebo-treated tumour 24 hours after surgery. C) Placebo-treated tumour 48 hours after ...read more
Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a ...read more
Smad1 deletion reduces oxidative stress and apoptosis following stroke.(A) Fluorescent images and quantification of in vivo HEt assay reflecting ROS levels on the ipsilateral hemisphere at 7 days post-stroke. The extent ...read more
Effects of IGF-1 or/and PDGF-bb on IL-1 beta -induced NF-kappa B-dependent pro-inflammatory, pro-apoptotic and matrix degrading gene products in chondrocytes.To determine whether IGF-1 or/and PDGF-bbexert effects on ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Caspase-3 Antibody [Unconjugated] - Active Summary

Immunogen
KLH-coupled human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # P42574
Specificity
Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
Reviewed Applications
Read 7 Reviews rated 4.9
using
AF835 in the following applications:

Publications
Read Publications using
AF835 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-3 Antibody [Unconjugated] - Active

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed, and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NB100-56118
Species: Ca, Ma, Hu, Mu, Rt
Applications: Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NBP2-27335
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
NB100-56116
Species: Ma, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
NB100-56104
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
NBP2-43728
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB120-13550
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAB1230
Species: Hu, Mu, Rt
Applications: ICC, IHC, KO, Simple Western, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB17761
Species: Hu, Mu, Rt
Applications: ICC, WB
AF8221
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF835
Species: Hu, Mu
Applications: IHC, ICC/IF

Publications for Caspase-3 Antibody (AF835)(210)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Canine, Chicken, Feline, Fish, Fish - Cyprinus carpio (Carp), Fish - Oncorhynchus mykiss (Rainbow Trout), Guinea Pig, Lamb, Ovine, Porcine, Primate, Transgenic Mouse, Zebrafish.

We have publications tested in 10 applications: Flow Cytometry, Functional Assay, ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(5)
Functional Assay
(1)
ICC
(44)
ICC/IF
(1)
IF
(2)
IHC
(107)
IHC-Fr
(11)
IHC-P
(36)
Immunoprecipitation
(1)
Western Blot
(11)
All Applications
Filter By Species
Human
(43)
Mouse
(134)
Rat
(10)
Canine
(1)
Chicken
(7)
Feline
(1)
Fish
(1)
Fish - Cyprinus carpio (Carp)
(1)
Fish - Oncorhynchus mykiss (Rainbow Trout)
(1)
Guinea Pig
(1)
Lamb
(1)
Ovine
(4)
Porcine
(4)
Primate
(1)
Transgenic Mouse
(2)
Zebrafish
(4)
All Species
Showing Publications 1 - 10 of 210. Show All 210 Publications.
Publications using AF835 Applications Species
Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse) IHC Mouse
Le, CT;Vick, LV;Collins, C;Dunai, C;Sheng, MK;Khuat, LT;Barao, I;Judge, SJ;Aguilar, EG;Curti, B;Dave, M;Longo, DL;Blazar, BR;Canter, RJ;Monjazeb, AM;Murphy, WJ; Regulation of human and mouse bystander T cell activation responses by PD-1 JCI insight 2023-09-22 [PMID: 37737264] (IHC, Mouse) IHC Mouse
Brockmueller, A;Girisa, S;Motallebi, M;Kunnumakkara, AB;Shakibaei, M; Calebin A targets the HIF-1?/NF-?B pathway to suppress colorectal cancer cell migration Frontiers in pharmacology 2023-07-31 [PMID: 37583906] (Western Blot, Human) Western Blot Human
Sinclair-Wilson, A;Lawrence, A;Ferezou, I;Cartonnet, H;Mailhes, C;Garel, S;Lokmane, L; Plasticity of thalamocortical axons is regulated by serotonin levels modulated by preterm birth Proceedings of the National Academy of Sciences of the United States of America 2023-08-15 [PMID: 37549297] (IHC, Mouse) IHC Mouse
Wang, D;Li, H;Chandel, NS;Dou, Y;Yi, R; MOF-mediated histone H4 Lysine 16 acetylation governs mitochondrial and ciliary functions by controlling gene promoters Nature communications 2023-07-21 [PMID: 37479688] (IHC, Mouse) IHC Mouse
Wei, H;Du, S;Parksong, J;Pasolli, HA;Matte-Martone, C;Regot, S;Gonzalez, LE;Xin, T;Greco, V; Organ function is preserved despite reorganization of niche architecture in the hair follicle Cell stem cell 2023-07-06 [PMID: 37419106] (IHC, Mouse) IHC Mouse
Rivera Alvarez, J;Asselin, L;Tilly, P;Benoit, R;Batisse, C;Richert, L;Batisse, J;Morlet, B;Levet, F;Schwaller, N;Mély, Y;Ruff, M;Reymann, AC;Godin, JD; The kinesin Kif21b regulates radial migration of cortical projection neurons through a non-canonical function on actin cytoskeleton Cell reports 2023-07-06 [PMID: 37418324] (IHC, Mouse) IHC Mouse
Gallini, S;Annusver, K;Rahman, NT;Gonzalez, DG;Yun, S;Matte-Martone, C;Xin, T;Lathrop, E;Suozzi, KC;Kasper, M;Greco, V; Injury prevents Ras mutant cell expansion in mosaic skin Nature 2023-06-21 [PMID: 37344586] (IHC, Mouse) IHC Mouse
Congiu, L;Granato, V;Jakovcevski, I;Kleene, R;Fernandes, L;Freitag, S;Kneussel, M;Schachner, M;Loers, G; Mice Mutated in the Third Fibronectin Domain of L1 Show Enhanced Hippocampal Neuronal Cell Death, Astrogliosis and Alterations in Behavior Biomolecules 2023-04-29 [PMID: 37238646] (IHC, Mouse) IHC Mouse
Kelly, SB;Dean, JM;Zahra, VA;Dudink, I;Thiel, A;Polglase, GR;Miller, SL;Hooper, SB;Bennet, L;Gunn, AJ;Galinsky, R; Progressive inflammation reduces high-frequency EEG activity and cortical dendritic arborisation in late gestation fetal sheep Journal of neuroinflammation 2023-05-24 [PMID: 37226206] (IHC, Ovine) IHC Ovine
Show All 210 Publications.

Reviews for Caspase-3 Antibody (AF835) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Mouse, Other.

Reviews using AF835:
Filter by Applications
WB
(1)
IHC-P
(4)
IF
(2)
All Applications
Filter by Species
Mouse
(6)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Mouse 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23374352
SpeciesMouse
  5
reviewed by:
Verified Customer
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20522641
SpeciesMouse
  5
reviewed by:
Verified Customer
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22897852
SpeciesMouse
  5
reviewed by:
Verified Customer
IHC-P Other 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22438018
SpeciesOther
  5
reviewed by:
Verified Customer
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23532769
SpeciesMouse
  5
reviewed by:
Verified Customer
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19226508
SpeciesMouse
  4
reviewed by:
Verified Customer
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23742999
SpeciesMouse

FAQs for Caspase-3 Antibody (AF835) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Caspase-3 Products

Diseases for Caspase-3 Antibody (AF835)

Discover more about diseases related to Caspase-3 Antibody (AF835).
 

Pathways for Caspase-3 Antibody (AF835)

View related products by pathway.

PTMs for Caspase-3 Antibody (AF835)

Learn more about PTMs related to Caspase-3 Antibody (AF835).

Blogs on Caspase-3. Showing 1-10 of 11 blog posts - Show all blog posts.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.


  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Cleaved Caspase-3: A Marker of Programmed Cell Death
What are Caspases?Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environme...  Read full blog post.

Caspase-3, The Executioner of Apoptosis
The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

 
Verified Customer
01/09/2015
Application: WB
Species: Mouse

 
Verified Customer
01/09/2015
Application: IHC-P
Species: Mouse

 
Verified Customer
01/09/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol CASP3
Uniprot