Caspase-3 Antibody - Active

Images

 
Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) ...read more
Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Caspase-3 Antibody - Active Summary

Immunogen
KLH-coupled human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # P42574
Specificity
Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 7 Reviews rated 4.9
using
AF835 in the following application:

Publications
Read Publications using
AF835 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-3 Antibody - Active

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-3 Antibody (AF835)(131)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Chicken, Feline, Fish, Fish - Cyprinus carpio (Carp), Guinea Pig, Ovine, Porcine, Primate, Zebrafish.

We have publications tested in 21 applications: Flow, Functional Assay, ICC, ICC Fluorescence, IF, IHC, IHC Frozen, IHC - Frozen, IHC - Not specified, IHC - PFA fixed, IHC - Paraffin embedded, IHC - paraffin, IHC Fresh, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, IHC-F, IHC-OCT embedded, IHC-P, WB.


Filter By Application
Flow
(3)
Functional Assay
(1)
ICC
(21)
ICC Fluorescence
(3)
IF
(1)
IHC
(43)
IHC Frozen
(1)
IHC - Frozen
(1)
IHC - Not specified
(2)
IHC - PFA fixed
(1)
IHC - Paraffin embedded
(5)
IHC - paraffin
(2)
IHC Fresh
(2)
IHC Frozen
(6)
IHC Not Specified
(6)
IHC OCT-embedded
(2)
IHC Paraffin-embedded
(19)
IHC-F
(1)
IHC-OCT embedded
(1)
IHC-P
(3)
WB
(6)
All Applications
Filter By Species
Human
(26)
Mouse
(80)
Rat
(5)
Chicken
(6)
Feline
(1)
Fish
(1)
Fish - Cyprinus carpio (Carp)
(1)
Guinea Pig
(1)
Ovine
(3)
Porcine
(3)
Primate
(1)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 131. Show All 131 Publications.
Publications using AF835 Applications Species
I Cohen, D Zhao, C Bar, VJ Valdes, KL Dauber-Dec, MB Nguyen, M Nakayama, M Rendl, WA Bickmore, H Koseki, D Zheng, E Ezhkova PRC1 Fine-tunes Gene Repression and Activation to Safeguard Skin Development and Stem Cell Specification Cell Stem Cell, 2018;22(5):726-739.e7. 2018 [PMID: 29727681] (IHC,, Mouse) IHC Mouse
R Barbieri, A Contestabi, MG Ciardo, N Forte, A Marte, P Baldelli, F Benfenati, F Onofri Synapsin I and Synapsin II regulate neurogenesis in the dentate gyrus of adult mice Oncotarget, 2018;9(27):18760-18774. 2018 [PMID: 29721159] (IHC-F,, Mouse) IHC-F Mouse
E Indersie, KB Hooks, C Capdeviell, M Fabre, N Dugot-Sena, A Desplat, S Lepreux, A Merched, CF Grosset, M Hagedorn Tracking cellular and molecular changes in a species-specific manner during experimental tumor progression in vivo Oncotarget, 2018;9(22):16149-16162. 2018 [PMID: 29662633] (IHC,, Human) IHC Human
PLN da Costa, D Wynne, T Fifis, L Nguyen, M Perini, C Christophi The kallikrein-Kinin system modulates the progression of colorectal liver metastases in a mouse model BMC Cancer, 2018;18(1):382. 2018 [PMID: 29618333] (IHC,, Mouse) IHC Mouse
N Antoniou, D Vlachakis, A Memou, E Leandrou, PE Valkimadi, K Melachroin, DB Re, S Przedborsk, WT Dauer, L Stefanis, HJ Rideout A motif within the armadillo repeat of Parkinson's-linked LRRK2 interacts with FADD to hijack the extrinsic death pathway Sci Rep, 2018;8(1):3455. 2018 [PMID: 29472595] (ICC,, Mouse) ICC Mouse
JM Povedano, P Martinez, R Serrano, Á Tejera, G Gómez-Lópe, M Bobadilla, JM Flores, F Bosch, MA Blasco Therapeutic effects of telomerase in mice with pulmonary fibrosis induced by damage to the lungs and short telomeres Elife, 2018;7(0):. 2018 [PMID: 29378675] (IHC-P,, Mouse) IHC-P Mouse
D Skuk, JP Tremblay The Process of Engraftment of Myogenic Cells in Skeletal Muscles of Primates: Understanding Clinical Observations and Setting Directions in Cell Transplantation Research Cell Transplant, 2017;26(11):1763-1779. 2017 [PMID: 29338383] (IF,, Primate) IF Primate
N Dreyer-And, AS Almeida, P Jensen, M Kamand, J Okarmus, T Rosenberg, SD Friis, A Martínez S, M Blaabjerg, BW Kristensen, T Skrydstrup, JB Gramsberge, HLA Vieira, M Meyer Intermittent, low dose carbon monoxide exposure enhances survival and dopaminergic differentiation of human neural stem cells PLoS ONE, 2018;13(1):e0191207. 2018 [PMID: 29338033] (ICC,, Human) ICC Human
CA Lyon, H Williams, R Bianco, SJ Simmonds, BA Brown, KS Wadey, FCT Smith, JL Johnson, SJ George Aneurysm Severity is Increased by Combined Mmp-7 Deletion and N-cadherin Mimetic (EC4-Fc) Over-Expression Sci Rep, 2017;7(1):17342. 2017 [PMID: 29229950] (IHC,, Human) IHC Human
MK Shim, HY Yoon, S Lee, MK Jo, J Park, JH Kim, SY Jeong, IC Kwon, K Kim Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis Sci Rep, 2017;7(1):16635. 2017 [PMID: 29192289] (WB,, Human) WB Human
Show All 131 Publications.

Reviews for Caspase-3 Antibody (AF835) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Mouse, Other.

Reviews using AF835:
Filter by Applications
WB
(1)
IHC-P
(4)
IF
(2)
All Applications
Filter by Species
Mouse
(6)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23374352
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23374352

Details

Detection NotesSee PMID 23374352

Comments

CommentsPublished: See PMID 23374352Independent review from antibodyresource.com
 
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20522641
SpeciesMouse
 
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22897852
SpeciesMouse
 
reviewed by:
Anonymous
IHC-P Other 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22438018
SpeciesOther
 
reviewed by:
Anonymous
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23532769
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23532769

Details

Detection NotesSee PMID 23532769

Comments

CommentsPublished: See PMID 23532769Independent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19226508
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19226508

Details

Detection NotesSee PMID 19226508

Comments

CommentsPublisheD: See PMID 19226508Independent review from antibodyresource.com
 
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23742999
SpeciesMouse

FAQs for Caspase-3 Antibody (AF835) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

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Blogs on Caspase-3. Showing 1-10 of 343 blog posts - Show all blog posts.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Showing 1-10 of 343 blog posts - Show all blog posts.

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Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

 
Anonymous
01/09/2015
Application: WB
Species: Mouse

 
Anonymous
01/09/2015
Application: IHC-P
Species: Mouse

 
Anonymous
01/09/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol CASP3
Entrez
Uniprot