Caspase-3 Antibody [Unconjugated] - Active

Images

 
Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody ...read more
Caspase‑3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) ...read more
Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Caspase-3 Antibody [Unconjugated] - Active Summary

Immunogen
KLH-coupled human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # P42574
Specificity
Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 7 Reviews rated 4.9
using
AF835 in the following applications:

Publications
Read Publications using
AF835 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-3 Antibody [Unconjugated] - Active

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed, and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-3 Antibody (AF835)(136)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Chicken, Feline, Fish, Fish - Cyprinus carpio (Carp), Guinea Pig, Ovine, Porcine, Primate, Zebrafish.

We have publications tested in 21 applications: Flow, Func, ICC, ICC Fluorescence, IF, IHC, IHC Frozen, IHC - Frozen, IHC - Not specified, IHC - PFA fixed, IHC - Paraffin embedded, IHC - paraffin, IHC Fresh, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, IHC-F, IHC-OCT embedded, IHC-P, WB.


Filter By Application
Flow
(4)
Func
(1)
ICC
(21)
ICC Fluorescence
(3)
IF
(1)
IHC
(42)
IHC Frozen
(1)
IHC - Frozen
(1)
IHC - Not specified
(2)
IHC - PFA fixed
(1)
IHC - Paraffin embedded
(6)
IHC - paraffin
(2)
IHC Fresh
(2)
IHC Frozen
(6)
IHC Not Specified
(6)
IHC OCT-embedded
(2)
IHC Paraffin-embedded
(19)
IHC-F
(1)
IHC-OCT embedded
(1)
IHC-P
(5)
WB
(7)
All Applications
Filter By Species
Human
(27)
Mouse
(81)
Rat
(6)
Chicken
(7)
Feline
(1)
Fish
(1)
Fish - Cyprinus carpio (Carp)
(1)
Guinea Pig
(1)
Ovine
(3)
Porcine
(3)
Primate
(1)
Zebrafish
(3)
All Species
Showing Publications 1 - 10 of 136. Show All 136 Publications.
Publications using AF835 Applications Species
O Bover, T Justo, PNG Pereira, J Facucho-Ol, JM Inácio, JS Ramalho, IJ Domian, JA Belo Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells PLoS ONE, 2018;13(10):e0205108. 2018 [PMID: 30281646] (Flow,, Mouse) Flow Mouse
M Ouarné, C Bouvard, G Boneva, C Mallet, J Ribeiro, A Desroches-, E Soleilhac, E Tillet, O Peyruchaud, S Bailly BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer J. Exp. Clin. Cancer Res., 2018;37(1):209. 2018 [PMID: 30165893] (IHC-P,, Mouse) IHC-P Mouse
A López-Muño, FE Nicolás, D García-Mor, AB Pérez-Oliv, MI Navarro-Me, MA Hernández-, A Herrera-Es, S Torres-Mar, RM Ruiz-Vázqu, V Garre, V Mulero An Adult Zebrafish Model Reveals that Mucormycosis Induces Apoptosis of Infected Macrophages Sci Rep, 2018;8(1):12802. 2018 [PMID: 30143654] (IHC - Paraffin embedded,, Zebrafish) IHC - Paraffin embedded Zebrafish
P Shen, J Chen, M Pan The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway Mol Med Rep, 2018;18(3):3332-3340. 2018 [PMID: 30066927] (WB,, Rat) WB Rat
M Baek, M Kim, JS Lim, LD Morales, J Hernandez, S Mummidi, S Williams-B, IS Jang, AT Tsin, DJ Kim Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNK signaling Cell Death Dis, 2018;9(7):730. 2018 [PMID: 29955047] (Human) Human
ML Piacentino, ME Bronner Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction PLoS Biol., 2018;16(6):e2004425. 2018 [PMID: 29949573] (IHC-P,, Chicken) IHC-P Chicken
I Cohen, D Zhao, C Bar, VJ Valdes, KL Dauber-Dec, MB Nguyen, M Nakayama, M Rendl, WA Bickmore, H Koseki, D Zheng, E Ezhkova PRC1 Fine-tunes Gene Repression and Activation to Safeguard Skin Development and Stem Cell Specification Cell Stem Cell, 2018;22(5):726-739.e7. 2018 [PMID: 29727681] (IHC,, Mouse) IHC Mouse
R Barbieri, A Contestabi, MG Ciardo, N Forte, A Marte, P Baldelli, F Benfenati, F Onofri Synapsin I and Synapsin II regulate neurogenesis in the dentate gyrus of adult mice Oncotarget, 2018;9(27):18760-18774. 2018 [PMID: 29721159] (IHC-F,, Mouse) IHC-F Mouse
E Indersie, KB Hooks, C Capdeviell, M Fabre, N Dugot-Sena, A Desplat, S Lepreux, A Merched, CF Grosset, M Hagedorn Tracking cellular and molecular changes in a species-specific manner during experimental tumor progression in vivo Oncotarget, 2018;9(22):16149-16162. 2018 [PMID: 29662633] (IHC,, Human) IHC Human
PLN da Costa, D Wynne, T Fifis, L Nguyen, M Perini, C Christophi The kallikrein-Kinin system modulates the progression of colorectal liver metastases in a mouse model BMC Cancer, 2018;18(1):382. 2018 [PMID: 29618333] (IHC,, Mouse) IHC Mouse
Show All 136 Publications.

Reviews for Caspase-3 Antibody (AF835) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Mouse, Other.

Reviews using AF835:
Filter by Applications
WB
(1)
IHC-P
(4)
IF
(2)
All Applications
Filter by Species
Mouse
(6)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23374352
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23374352

Details

Detection NotesSee PMID 23374352

Comments

CommentsPublished: See PMID 23374352Independent review from antibodyresource.com
 
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20522641
SpeciesMouse
 
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22897852
SpeciesMouse
 
reviewed by:
Anonymous
IHC-P Other 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22438018
SpeciesOther
 
reviewed by:
Anonymous
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23532769
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23532769

Details

Detection NotesSee PMID 23532769

Comments

CommentsPublished: See PMID 23532769Independent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19226508
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19226508

Details

Detection NotesSee PMID 19226508

Comments

CommentsPublisheD: See PMID 19226508Independent review from antibodyresource.com
 
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23742999
SpeciesMouse

FAQs for Caspase-3 Antibody (AF835) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

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Blogs on Caspase-3.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

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Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

 
Anonymous
01/09/2015
Application: WB
Species: Mouse

 
Anonymous
01/09/2015
Application: IHC-P
Species: Mouse

 
Anonymous
01/09/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol CASP3
Uniprot