ATG7 Antibody (683906) [Unconjugated]


Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody more
ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained more
ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the more
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # MAB002, open more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 72 kDa (as indicated) using 20 µg/mL of Mouse more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF

Order Details

ATG7 Antibody (683906) [Unconjugated] Summary

E. coli-derived recombinant human ATG7
Accession # O95352
Detects human ATG7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 10, 12, or 16L1 is observed.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
MAB6608 in the following applications:

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MAB6608 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ATG7 Antibody (683906) [Unconjugated]

  • APG7 autophagy 7-like (S. cerevisiae)
  • APG7
  • APG7L
  • ATG12-activating enzyme E1 ATG7
  • ATG7 autophagy related 7 homolog (S. cerevisiae)
  • ATG7
  • Autophagy-related protein 7
  • DKFZp434N0735
  • GSA7
  • hAGP7
  • ubiquitin activating enzyme E1-like protein
  • Ubiquitin-activating enzyme E1-like protein
  • ubiquitin-like modifier-activating enzyme ATG7


ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin-E1-like enzyme that plays a key role in the autophagic pathway of intracellular bulk degradation. It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8, and subsequent autophagosome formation. ATG7 is required for mitochondrial removal during erythropoiesis and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: IHC, IHC-P, IP, WB
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Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, S-ELISA, WB
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Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
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Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Bv, Hu, Ma
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Pm, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF

Publications for ATG7 Antibody (MAB6608)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Flow Cytometry, IHC, Western Blot.

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Flow Cytometry
Western Blot
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Showing Publications 1 - 8 of 8.
Publications using MAB6608 Applications Species
D Beillevair, F Migneault, J Turgeon, D Gingras, AK Rimbaud, G Marcoux, C Spino, N Thibodeau, E Bonneil, P Thibault, É Boilard, M Dieudé, MJ Hébert Autolysosomes and caspase-3 control the biogenesis and release of immunogenic apoptotic exosomes Cell Death & Disease, 2022-02-11;13(2):145. 2022-02-11 [PMID: 35149669] (Western Blot, Human) Western Blot Human
K Pandey, XW Yu, A Steinmetz, CM Alberini Autophagy coupled to translation is required for long-term memory Autophagy, 2020-06-17;0(0):. 2020-06-17 [PMID: 32501746] (Western Blot, Rat) Western Blot Rat
YH Sheng, R Giri, J Davies, V Schreiber, S Alabbas, R Movva, Y He, A Wu, J Hooper, B McWhinney, I Oancea, G Kijanka, S Hasnain, AJ Lucke, DP Fairlie, MA McGuckin, TH Florin, J Begun A Nucleotide Analog Prevents Colitis-Associated Cancer via Beta-Catenin Independently of Inflammation and Autophagy Cell Mol Gastroenterol Hepatol, 2020-06-01;0(0):. 2020-06-01 [PMID: 32497793] (Western Blot, Human) Western Blot Human
M Bernard, B Yang, F Migneault, J Turgeon, M Dieudé, MA Olivier, GB Cardin, M El-Diwany, K Underwood, F Rodier, MJ Hébert Autophagy drives fibroblast senescence through MTORC2 regulation Autophagy, 2020-01-13;0(0):1-13. 2020-01-13 [PMID: 31931659] (Western Blot, Human) Western Blot Human
S Jung, S Choe, H Woo, H Jeong, HK An, H Moon, HY Ryu, BK Yeo, YW Lee, H Choi, JY Mun, W Sun, HK Choe, EK Kim, SW Yu Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits Autophagy, 2019-06-24;0(0):1-19. 2019-06-24 [PMID: 31234698] (IHC, Mouse) IHC Mouse
P Thapa, S Romero Aro, JY Chung, DB Sant'Angel, VS Shapiro Histone deacetylase 3 is required for iNKT cell development Sci Rep, 2017-07-19;7(1):5784. 2017-07-19 [PMID: 28724935] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Maurizio Bossola Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia Exp. Gerontol., 2016-11-12;0(0):. 2016-11-12 [PMID: 27847330] (Western Blot, Human) Western Blot Human
Bernard M, Dieude M, Yang B, Hamelin K, Underwood K, Hebert M Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF. Autophagy, 2014-01-01;10(12):2193-207. 2014-01-01 [PMID: 25495560] (Western Blot, Human) Western Blot Human

Review for ATG7 Antibody (MAB6608) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB6608:
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Images Ratings Applications Species Date Details
Western Blot ATG7 MAB6608
reviewed by:
Julian Nuechel
WB Human 07/12/2017


ApplicationWestern Blot
Sample TestedHuman fibroblast cell line


Commentsfor WB: blocking 1 h 5% skimmed milk in TBST; Ab o/n @ 4 °C 1:1000 in TBST. Beclin1-specific siRNA-transfected cells do not show a signal.

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Blogs on ATG7. Showing 1-10 of 13 blog posts - Show all blog posts.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Autophagic flux: Is p62 a good indicator?
By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to aut...  Read full blog post.

Best genes to knockout to control autophagic flux
By Christina Towers, PhD Autophagy flux: Basic principlesAutophagic flux is defined as the amount of cellular material degraded and recycled through the process of autophagy, whereby cells break down and disca...  Read full blog post.

The LC3 A, B, C’s and 1, 2, 3’s
By Christina Towers, PhD Autophagy is a catabolic process used to breakdown and recycle damaged proteins and organelles. It is a multistep process that, in its simplest form, consists of 4 steps: initiation, phago...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

Monitoring Autophagy in Neurons
By Christina Towers, PhD. Autophagy is a critical cellular process used by most cells in the body to recycle nutrients and prevent harmful buildup of damaged proteins. It is particularly important in the brain, where ...  Read full blog post.

Autophagy independent roles of the core ATG proteins
By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation.  It is critical for removal of dam...  Read full blog post.

Nuclear LC3: Why is it there and what is it doing?
By Christina Towers, PhD. Cells use the complex process of autophagy to degrade and recycle cytoplasmic material.  There are over 20 proteins that have been implicated in this process and appropriately named core ...  Read full blog post.

From Then ‘till Now: The History of Autophagy and Cancer Research
By Christina Towers, PhD. The fundamental process that cells use to degrade damaged cytoplasmic material and recycle nutrients is called autophagy.  This term was first coined by the Belgium biochemist Christian de...  Read full blog post.

ATG4B - a cysteine protease involved in autophagosome elongation
Autophagy can be broken down into 4 main stages: phagophore nucleation, autophagosome elongation, autophagosome docking and fusion with a lysosome, and vesicle breakdown and degradation. ATG4B is one of four ATG4 homologs (ATG4A, ATG4B, ATG4C, and ...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.
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Recent Reviews


Julian Nuechel
Application: WB
Species: Human


Gene Symbol ATG7