NUP62 Antibody

Western Blot: NUP62 Antibody [NBP1-31381] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Nucleoporin p62 Antibody [NBP1-31381] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody diluted at 1:750. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Nucleoporin p62 Antibody [NBP1-31381] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

Western Blot: Nucleoporin p62 Antibody [NBP1-31381] - PC-12 whole cell lysate (30 ug) stained wtih Nucleoporin p62 Antibody diluted at 1:5000.

Western Blot: Nucleoporin p62 Antibody [NBP1-31381] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 7. 5% SDS PAGE; antibody diluted at 1:5000.

Immunoprecipitation: Nucleoporin p62 Antibody [NBP1-31381] - A. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of NUP62 protein by 4 ug Nucleoporin p62 antibody 7.5 % SDS-PAGE The immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody diluted at 1:1000.[EasyBlot anti-rabbit IgG) was used as a secondary reagent]

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NUP62 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Nucleoporin p62. The exact sequence is proprietary.
• Localization: Nucleus, nuclear pore complex
• Marker: Nuclear Pore Marker
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUP62
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for NUP62 Antibody

• DKFZp547L134,62 kDa nucleoporin
• FLJ20822
• FLJ43869
• IBSNp62
• MGC841
• nuclear pore glycoprotein p62
• nucleoporin 62kD
• nucleoporin 62kDa
• SNDINucleoporin Nup62

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NUP62 Antibody (NBP1-31381)(4)

Publications using NBP1-31381

• Peng K, Liu R, Jia C et al. Regulation of O-Linked N-Acetyl Glucosamine Transferase (OGT) through E6 Stimulation of the Ubiquitin Ligase Activity of E6AP International Journal of Molecular Sciences 2021-09-24 [PMID: 34638625]
• Lee S, Moon H, Lee Y et al. Potentiation of TRAIL induced cell death by nonsteroidal anti inflammatory drug in human hepatocellular carcinoma cells through the ER stress dependent autophagy pathway Oncol Rep 2020-06-25 [PMID: 32705218] (WB, Human)
• Sun H, Huang M, Yao N, Hu J et al. The cycloartane triterpenoid ADCX impairs autophagic degradation through Akt overactivation and promotes apoptotic cell death in multidrug-resistant HepG2/ADM cells Biochem. Pharmacol. 2017-10-23 [PMID: 29074104] (WB, Human)
• Rossi A, Coum A, Madelenat M, et al. Neural stem cells alter nucleocytoplasmic partitioning and accumulate nuclear polyadenylated transcripts during quiescence bioRxiv 2021-01-07 (WB)

Bioinformatics

• Gene Symbol: NUP62
• Entrez:
  • 23636(Human)
• Uniprot:
  • P37198(Human)