HIF-1 alpha Antibody Pack

Images

 
Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.
Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy ...read more
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody Pack [NB100-905] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.
Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of HIF1-alpha in CoCl2 Treated vs. Untreated HeLa Cells. Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. ...read more
Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha in Cobalt Chloride treated/untreated Cos-7 nuclear extracts using NB100-654.
Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of mouse HIF1-alpha on hypoxia treated MEFs using NB 100-449.
Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic).
Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, ...read more
Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. ...read more
Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. ...read more
Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. Photos courtesy of Susan Alexander ...read more
Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining of mouse brain, neurons using NB100-449.
Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining of placenta, villi using NB100-479.
Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Immunohistochemical staining of human glioblastoma multiforme utilizing NB100-105.
Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 Alpha staining in hypoxia-induced human placenta using NB 100-131.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Order Details

HIF-1 alpha Antibody Pack Summary

Immunogen
NB 100-654 = Human HIF-1 alpha amino acids 432-528. NB 100-105 = Human HIF-1 alpha amino acids 432-528. NB 100-131 = Human HIF-1 alpha amino acids 329-530. NB 100-449 = Human HIF-1 alpha amino acids 775-826. NB 100-479 = Mouse HIF-1 alpha amino acids
Specificity
These antibodies are specific for HIF-1alpha.
Gene
HIF1A

Applications/Dilutions

Application Notes
Western Blot: All antibodies included in this pack will work for Western analysis, except for NB100-131, which has mixed results for Western analysis. A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background. Immunohistochemistry: Both positive and negative feedback have been provided on all antibodies included in this pack. A starting dilution of 1:250 is recommended with titration up or down depending upon the signal and background.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-905 in the following application:

Publications
Read Publications using
NB100-905 in the following applications:

  • IHC
    1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

NB 100-654 recognizes human, mouse and rat. NB 100-105 recognizes human, sheep, mouse, rat, porcine, rabbit, bovine and ferret HIF-1 alpha. NB 100-131 recognizes human, mouse and rat HIF-1 alpha. NB 100-449 recognizes human and mouse HIF-1 alpha. Prelimary results with mouse tissue have been positive for IHC using mouse eye sections at 1:100 with high pH retrieval. NB 100-479 recognizes human and mouse HIF-1 alpha.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Notes

1 vial each of: NB100-654SS (0.025ml), NB100-479SS (0.025ml), NB100-449SS (0.025ml), NB100-105 (0.05mL) and NB100-131 (0.05mL).

Alternate Names for HIF-1 alpha Antibody Pack

  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • BHLHE78
  • Class E basic helix-loop-helix protein 78
  • HIF 1A
  • HIF1 alpha
  • HIF-1 alpha
  • HIF1A
  • HIF-1a
  • HIF-1-alpha
  • HIF1-alpha
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor)
  • hypoxia-inducible factor 1-alpha
  • Member of PAS protein 1
  • member of PAS superfamily 1
  • MOP1HIF1-ALPHA
  • PAS domain-containing protein 8
  • PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)

Background

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein that activates gene transcription in response to reduced cellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, heme oxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is a heterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced by hypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulate hypoxia-inducible factors may be related to tumor-related angiogenesis in certain cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
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NB300-605
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NB100-137
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Publications for HIF-1 alpha Antibody Pack (NB100-905)(4)

Reviews for HIF-1 alpha Antibody Pack (NB100-905) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.

Reviews using NB100-905:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot HIF-1 alpha NB100-905
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reviewed by:
Ming Lu
WB Human 08/08/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell lines
SpeciesHuman
LotG
CommentsAlthough it is a monoclonal antibody, it has a little dirty background. But it shows strong band at correct MW.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Cell Signaling, #7076
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives strong band at correct MW.

Comments

CommentsAlthough it is a monoclonal antibody, it has a little dirty background. But it shows strong band at correct MW.
Western Blot HIF-1 alpha NB100-905
Enlarge
reviewed by:
Ming Lu
WB Human 08/05/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman Pancreatic Cancer Cell line
SpeciesHuman
LotC-2
CommentsThis antibody is very easy to work with. Under hypoxia condition, the HIF1alpha signal is very strong.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Cell Signaling, #7074
Secondary Concentration1:5000

Details

Detection Notescells were treated under normoxia and hypoxia condition for 24h

Comments

CommentsThis antibody is very easy to work with. Under hypoxia condition, the HIF1alpha signal is very strong.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for HIF-1 alpha Antibody Pack (NB100-905). (Showing 1 - 4 of 4 FAQs).

  1. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
    • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
  2. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
    • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, as desired by you, I am suggesting you four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products has been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449 ) (cited in at least 31 peer reviewed publications).
  3. I would like to know, does it exist path for detection HIF 1 in the venous blood before and after revascularization of the leg?
    • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
  4. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
    • The theoretical molecular weight of HIF 1-alpha is ~93kDa, however, you will likely see a band between 100-120kDa due to phosphorylation.

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Blogs on HIF-1 alpha. Showing 1-10 of 27 blog posts - Show all blog posts.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Controls for HIF-1 Alpha's WB, ICC-IF, IHC, IP & FLOW Analysis
Tips on positive and negative controls for HIF-1 alpha antibodies is one of the most Frequently Asked Questions on Hypoxia and HIFs. Here are top 5 suggestions from Novus Biologicals: The degradation of HIF1 alpha is the most common issue whic...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

Comprehensive Autophagy Research Tools - New Catalog Available Now!
Autophagy, a protein degradation process through autophagosome-lysosomal pathway, is important for cellular homeostasis and plays a role in many diseases. To help researchers learn more about this process and the products available for its study, N...  Read full blog post.

HIF-1 beta: A dimerization partner of HIF-1 alpha required for an adaptive response to hypoxia
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia-indu...  Read full blog post.

Showing 1-10 of 27 blog posts - Show all blog posts.

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Recent Reviews

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Ming Lu
08/08/2014
Application: WB
Species: Human

Ming Lu
08/05/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIF1A
Entrez