TLR2 Antibody

Images

 
Western Blot: TLR2 Antibody [NB100-56720] - Analysis using the Azide Free version of NB100-56720. Detection of TLR2 in transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: TLR2 Antibody [NB100-56720] - analysis of TLR2 in paraffin-embedded formalin-fixed mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Formulation Size Price

TLR2 Antibody Summary

Immunogen
This antibody was developed against a mixture of synthetic peptides containing amino acids 180-196, 353-370, and 473-489 of human TLR2 (NP_003255).
Specificity
In transfected human TLR2 cell lysate, a 90 kDa band is observed. This has also been reported to work well in bronchial epithelial cells (UBH16E) and THP-1 cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Agonist
Antagonist
Positive Control
TLR2 Lysate (NBL1-16952)
Control Peptide
TLR2 Peptide (NB100-56720PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56720 in the following applications:

Publications
Read Publications using
NB100-56720 in the following applications:

  • 2 publications
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR2 Antibody

  • CD282 antigen
  • CD282
  • TIL4CD282
  • TLR2
  • toll/interleukin 1 receptor-like 4
  • Toll/interleukin-1 receptor-like protein 4
  • toll-like receptor 2

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-56720
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Supplier Logo

Publications for TLR2 Antibody (NB100-56720)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(2)
WB
(7)
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Human
(6)
Mouse
(4)
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Showing Publications 1 - 10 of 10.
Publications using NB100-56720 Applications Species
Sumedha S, Kotrashetti VS, Nayak RS, Nayak A et al. Immunohistochemical localization of TLR2 and CD14 in gingival tissue of healthy individuals and patients with chronic periodontitis Biotech Histochem 2017 Sep 14 [PMID: 28910171] (Human) Human
Trune DR, Kempton B, Hausman FA et al. Correlative mRNA and protein expression of middle and inner ear inflammatory cytokines during mouse acute otitis media Hear. Res. 2015 [PMID: 25922207] (IHC-P, Mouse) IHC-P Mouse
Komala Mg, Gross S, Mudaliar H et al. Inhibition of Kidney Proximal Tubular Glucose Reabsorption Does not Prevent against Diabetic nephropathy in Type 1 Diabetic enOS Knockout Mice PloS one et al. 2014 Nov 04 [PMID: 25369239] (IHC-P, Mouse)

Details:
TLR2 antibody used at 1:2000 dilution in IHC-P on formalin fixed paraffin embedded kidney tissue sections from experiments involving streptozotocin induced Type 1 Diabetic enOS Knockout Mice (Figure 3).
IHC-P Mouse
Moreth K, Frey H, Hubo M et al. Biglycan-triggered TLR-2- and TLR-4-signaling exacerbates the pathophysiology of ischemic acute kidney injury. Matrix Biol. 2014 Jan 28 [PMID: 24480070] (WB, Mouse)

Details:
Murine peritoneal macrophages, Fig 1C. Cells were IPd with biglycan, followed by WB with the TLR2 pAb. The IP/WB results showed that that TLR2 co-IPd with biglycan, indicating protein-protein interactions between biglycan and TLR2. TLR2 was detected at 90
WB Mouse
Krishnan S, Chen S, Turcatel G et al. Regulation of Toll-like receptor 2 interaction with Ecgp96 controls Escherichia coli K1 invasion of brain endothelial cells Cell Microbiol. 2013 Jan [PMID: 22963587] (WB, Human) WB Human
Su X, Ao L, Shi Y et al. Oxidized low density lipoprotein induces bone morphogenetic protein-2 in coronary artery endothelial cells via Toll-like receptors 2 and 4. J Biol Chem. 2011 Apr 8 [PMID: 21325271] (WB, Human)

Details:
TLR2/CD282 (IMG-410A). WB: TLR2 siRNA transfected human coronary artery endothelial (CAECs) cell line, Fig 3A. Note: The TLR2 (IMG-410A) antibody was siRNA validated by WB in CAEC cell line, Fig 3A.
WB Human
Ghanim H, Sia CL, Upadhyay M et al. Orange juice neutralizes the proinflammatory effect of a high-fat, high-carbohydrate meal and prevents endotoxin increase and Toll-like receptor expression. Am J Clin Nutr. 2010 Apr [PMID: 20200256] (WB, Human)

Details:
TLR2 (IMG-410A): WB (human mononuclear cells), Fig 6A.
WB Human
Wu H, Wang H, Xiong W et al. Expression patterns and functions of toll-like receptors in mouse sertoli cells. Endocrinology. 2008 Sep [PMID: 18499758] (WB, Mouse)

Details:
Antibodies cited: 1. TLR2 (IMG-410A): WB (sertoli cell lysates), Fig. 1D. 2. TLR5 (IMG-444A): WB (sertoli cell lysates), Fig. 1D. 3. TLR5 (IMG-664C): Flow (cell surface), mouse sertoli cells and macrophages, Figs. 1B,C,E. 4. TLR6 (IMG-527): WB (sertoli ce
WB Mouse
Chun J, Prince A. Activation of Ca2+-dependent signaling by TLR2. J Immunol. 2006 Jul 15 [PMID: 16818794] (WB, Human)

Details:
1. TLR2 (IMG-410) [WB (human airway epithelial cell lines)].
WB Human
LeBouder E, Rey-Nores JE, Rushmere NK et al. Soluble forms of Toll-like receptor (TLR)2 capable of modulating TLR2 signaling are present in human plasma and breast milk. J Immunol. 2003 Dec 15 [PMID: 14662871] (WB, Human)

Details:
1. TLR2 (IMG-319 and IMG-410); For IMG-319: WB, Fig.1D and Fig.6C (Mono Mac-6 monocytes); For IMG-410: Fig.6A-B, (human breast milk).
WB Human

Review for TLR2 Antibody (NB100-56720) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56720:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/15/2014
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Summary

ApplicationWestern Blot
Sample TestedSee PMID: 22963587
SpeciesHuman
CommentsIndependent review from antibodyresource.com. Published in PMID: 22963587.

Blocking

Blocking DetailsSee PMID: 22963587

Details

Detection NotesSee PMID: 22963587

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 22963587.

Product General Protocols

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FAQs for TLR2 Antibody (NB100-56720) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TLR2 Antibody (NB100-56720)

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Blogs on TLR2.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR1
TLR1 belongs to the Toll-like receptor (TLR) family, and is a key player in the recognition of pathogens as well as the activation of the innate immunity system. TLRs are highly conserved proteins with a high degree of structural and functional homolo...  Read full blog post.

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Recent Reviews

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Anonymous
12/15/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TLR2
Entrez
Uniprot