TLR2 Antibody


Biological Strategies: Immunohistochemistry: TLR2 Antibody [NB100-56720] - Representative photographs of immunohistochemistry for tubular TLR2 in G) ctrl, H) ctrl + empa, I) diabetic (dm), J) dm + empagliflozin, more
Western Blot: TLR2 Antibody [NB100-56720] - Analysis using the Azide Free version of NB100-56720. Detection of TLR2 in transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: TLR2 Antibody [NB100-56720] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml
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Biological Strategies


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TLR2 Antibody Summary

This antibody was developed against a mixture of synthetic peptides containing amino acids 180-196, 353-370, and 473-489 of human TLR2 (NP_003255).
In transfected human TLR2 cell lysate, a 90 kDa band is observed. This has also been reported to work well in bronchial epithelial cells (UBH16E) and THP-1 cells.
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1-3 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30618413). Use in FLOW reported in scientific literature (PMID: 31285077). Use in immunoprecipitation reported in scientific literature (PMID:31383741).
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
TLR2 Overexpression Lysate
Control Peptide
TLR2 Peptide (NB100-56720PEP)
Reviewed Applications
Read 1 Review rated 5
NB100-56720 in the following applications:

Read Publications using
NB100-56720 in the following applications:

  • 1 publication
  • 1 publication
  • 3 publications
  • IP
    1 publication
  • WB
    9 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for TLR2 Antibody

  • CD282 antigen
  • CD282
  • TIL4CD282
  • TLR2
  • toll/interleukin 1 receptor-like 4
  • Toll/interleukin-1 receptor-like protein 4
  • toll-like receptor 2


The Toll-like receptor (TLR) family in mammals comprises a set of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain, and in the IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, IHC, IHC-P, KO
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Applications: WB, DB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
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Applications: WB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
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Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu, Mu, Ca
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-CS, Flow-IC
Species: Hu, Mu, Rt
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Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, BA, Flow-IC
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Publications for TLR2 Antibody (NB100-56720)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow, ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-56720 Applications Species
Khandelwal N, Shaikh M, Mhetre A et al. Fatty acid chain length drives lysophosphatidylserine-dependent immunological outputs Cell chemical biology Jan 21 2021 [PMID: 33571455]
Eyking, A;Ferber, F;Köhler, S;Reis, H;Cario, E; TRIM58 Restrains Intestinal Mucosal Inflammation by Negatively Regulating TLR2 in Myeloid Cells J. Immunol. Aug 5 2019 [PMID: 31383741] (IP, WB, Human) IP, WB Human
Arya RP, Arankalle VA Phenotypic analysis of monocytes and CD4+ T cells in hepatitis E patients with or without pregnancy Hum. Immunol. Jul 5 2019 [PMID: 31285077] (Flow, Human) Flow Human
Fan Y, Yang L, Wei Q et al. Toll-like receptor 10 (TLR10) exhibits suppressive effects on inflammation of prostate epithelial cells. Asian J. Androl. Jan 1 2019 [PMID: 30618413] (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human
Zou Weifeng, He Fang, Liu Sha et al. PM2.5 Induced the Expression of Fibrogenic Mediators via HMGB1-RAGE Signaling in Human Airway Epithelial Cells. Canadian RespiRatory Journal 2018 [PMID: 29670673] (WB, Human) WB Human
Sumedha S, Kotrashetti VS, Nayak RS, Nayak A et al. Immunohistochemical localization of TLR2 and CD14 in gingival tissue of healthy individuals and patients with chronic periodontitis Biotech Histochem 2017 Sep 14 [PMID: 28910171] (Human) Human
Trune DR, Kempton B, Hausman FA et al. Correlative mRNA and protein expression of middle and inner ear inflammatory cytokines during mouse acute otitis media Hear. Res. 2015 [PMID: 25922207] (IHC-P, Mouse) IHC-P Mouse
Komala Mg, Gross S, Mudaliar H et al. Inhibition of Kidney Proximal Tubular Glucose Reabsorption Does not Prevent against Diabetic nephropathy in Type 1 Diabetic enOS Knockout Mice PloS one et al. 2014 Nov 04 [PMID: 25369239] (IHC-P, Mouse)

TLR2 antibody used at 1:2000 dilution in IHC-P on formalin fixed paraffin embedded kidney tissue sections from experiments involving streptozotocin induced Type 1 Diabetic enOS Knockout Mice (Figure 3).
IHC-P Mouse
Moreth K, Frey H, Hubo M et al. Biglycan-triggered TLR-2- and TLR-4-signaling exacerbates the pathophysiology of ischemic acute kidney injury. Matrix Biol. 2014 Jan 28 [PMID: 24480070] (WB, Mouse)

Murine peritoneal macrophages, Fig 1C. Cells were IPd with biglycan, followed by WB with the TLR2 pAb. The IP/WB results showed that that TLR2 co-IPd with biglycan, indicating protein-protein interactions between biglycan and TLR2. TLR2 was detected at 90
WB Mouse
Krishnan S, Chen S, Turcatel G et al. Regulation of Toll-like receptor 2 interaction with Ecgp96 controls Escherichia coli K1 invasion of brain endothelial cells Cell Microbiol. 2013 Jan [PMID: 22963587] (WB, Human) WB Human
Show All 15 Publications.

Review for TLR2 Antibody (NB100-56720) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56720:
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Images Ratings Applications Species Date Details
reviewed by:
Verified Customer
WB Human 12/15/2014


ApplicationWestern Blot
Sample TestedSee PMID: 22963587
CommentsIndependent review from Published in PMID: 22963587.


Blocking DetailsSee PMID: 22963587


Detection NotesSee PMID: 22963587


CommentsIndependent review from antibodyresource.comPublished in PMID: 22963587.

Product General Protocols

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Control Lysate(s)

Secondary Antibodies


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Blogs on TLR2.

  Read full blog post.

Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance
By Jamshed Arslan, Pharm. D., PhD. Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Peri...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR1 belongs to the Toll-like receptor (TLR) family, and is a key player in the recognition of pathogens as well as the activation of the innate immunity system. TLRs are highly conserved proteins with a high degree of structural and functional homolo...  Read full blog post.

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Verified Customer
Application: WB
Species: Human


Gene Symbol TLR2