TLR2 Antibody

Immunohistochemistry: TLR2 Antibody [NB100-56720] - Representative photographs of immunohistochemistry for tubular TLR2 in G) ctrl, H) ctrl + empa, I) diabetic (dm), J) dm + empagliflozin, K) dm + telmisartan groups (Magnification = original X 200) and quantification of tubular TLR2 expression by Image J (L). (Data are expressed as mean +/- SEM with *=P<0.05 vs ctrl, **=P<0.001 vs ctrl and #=P<0.05 vs dm). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0108994) licensed under a CC-BY license.

Western Blot: TLR2 Antibody [NB100-56720] - Analysis using the Azide Free version of NB100-56720. Detection of TLR2 in transfected cell lysate using this antibody.

Immunohistochemistry-Paraffin: TLR2 Antibody [NB100-56720] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TLR2 Antibody Summary

• Immunogen: This antibody was developed against a mixture of synthetic peptides containing amino acids 180-196, 353-370, and 473-489 of human TLR2 (NP_003255).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TLR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 31285077)
  • Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 30618413)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation reported in scientific literature (PMID 31383741)
  • Western Blot 1-3 ug/ml
• Application Notes: In transfected human TLR2 cell lysate, a 90 kDa band is observed. This has also been reported to work well in bronchial epithelial cells (UBH16E) and THP-1 cells.
• Theoretical MW: 89 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Agonist:
  • Pam3CSK4, TLR1 and TLR2 Ligand NBP2-25297
  • SUCNR1/GPR91 Antibody NBP2-26239
  • MALP-2, TLR6 and TLR2 ligand NBP2-26219
• Antagonist:
  • TIRAP (TLR2 and TLR4) Inhibitor Peptide Set NBP2-29331
• Control:

  TLR2 Overexpression Lysate

• Control Peptide:

  TLR2 Peptide (NB100-56720PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TLR2 Antibody

• CD282 antigen
• CD282
• TIL4CD282
• TLR2
• toll/interleukin 1 receptor-like 4
• Toll/interleukin-1 receptor-like protein 4
• toll-like receptor 2

Background

The Toll-like receptor (TLR) family in mammals comprises a set of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain, and in the IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR2 Antibody (NB100-56720)(18)

Publications using NB100-56720

• Do?an G, Karagenç N, Esmen K et al. Expression of Toll-Like Receptors in the Lung Tissue of Mouse Fetuses Generated by in vitro Embryo Culture and Embryo Transfer Cells, tissues, organs 2023-04-27 [PMID: 37105136] (WB, Mouse)
• Millar FR, Pennycuick A, Muir M Et al. Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer Cell Rep 2022-11-09 [PMID: 36351380] (IHC-P, Human, Mouse)
  
  Details:
  Citation using the Azide Free version of this antibody.
• Yoshimoto T, Kittaka M, Doan AAP et al. Osteocytes directly regulate osteolysis via MYD88 signaling in bacterial bone infection Nature communications 2022-11-04 [PMID: 36333322] (IHC-P, Mouse)
• Khandelwal N, Shaikh M, Mhetre A et al. Fatty acid chain length drives lysophosphatidylserine-dependent immunological outputs Cell chemical biology 2021-01-21 [PMID: 33571455]
• Eyking, A;Ferber, F;Köhler, S;Reis, H;Cario, E; TRIM58 Restrains Intestinal Mucosal Inflammation by Negatively Regulating TLR2 in Myeloid Cells J. Immunol. 2019-08-05 [PMID: 31383741] (WB, IP, Human)
• Arya RP, Arankalle VA Phenotypic analysis of monocytes and CD4+ T cells in hepatitis E patients with or without pregnancy Hum. Immunol. 2019-07-05 [PMID: 31285077] (FLOW, Human)
• Fan Y, Yang L, Wei Q et al. Toll-like receptor 10 (TLR10) exhibits suppressive effects on inflammation of prostate epithelial cells. Asian J. Androl. 2019-01-01 [PMID: 30618413] (ICC/IF, IHC-P, Human)
• Zou Weifeng, He Fang, Liu Sha et al. PM2.5 Induced the Expression of Fibrogenic Mediators via HMGB1-RAGE Signaling in Human Airway Epithelial Cells. Canadian RespiRatory Journal 2018-01-01 [PMID: 29670673] (WB, Human)
• Sumedha S, Kotrashetti VS, Nayak RS, Nayak A et al. Immunohistochemical localization of TLR2 and CD14 in gingival tissue of healthy individuals and patients with chronic periodontitis Biotech Histochem 2017-09-14 [PMID: 28910171] (Human)
• Trune DR, Kempton B, Hausman FA et al. Correlative mRNA and protein expression of middle and inner ear inflammatory cytokines during mouse acute otitis media Hear. Res. 2015-01-01 [PMID: 25922207] (IHC-P, Mouse)
• Komala Mg, Gross S, Mudaliar H et al. Inhibition of Kidney Proximal Tubular Glucose Reabsorption Does not Prevent against Diabetic nephropathy in Type 1 Diabetic enOS Knockout Mice PloS one et al. 2014-11-04 [PMID: 25369239] (IHC-P, Mouse)
  
  Details:
  TLR2 antibody used at 1:2000 dilution in IHC-P on formalin fixed paraffin embedded kidney tissue sections from experiments involving streptozotocin induced Type 1 Diabetic enOS Knockout Mice (Figure 3).
• Moreth K, Frey H, Hubo M et al. Biglycan-triggered TLR-2- and TLR-4-signaling exacerbates the pathophysiology of ischemic acute kidney injury. Matrix Biol. 2014-01-28 [PMID: 24480070] (WB, Mouse)
  
  Details:
  Murine peritoneal macrophages, Fig 1C. Cells were IPd with biglycan, followed by WB with the TLR2 pAb. The IP/WB results showed that that TLR2 co-IPd with biglycan, indicating protein-protein interactions between biglycan and TLR2. TLR2 was detected at 90
• Krishnan S, Chen S, Turcatel G et al. Regulation of Toll-like receptor 2 interaction with Ecgp96 controls Escherichia coli K1 invasion of brain endothelial cells Cell Microbiol. 2013-01-01 [PMID: 22963587] (WB, Human)
• Su X, Ao L, Shi Y et al. Oxidized low density lipoprotein induces bone morphogenetic protein-2 in coronary artery endothelial cells via Toll-like receptors 2 and 4. J Biol Chem. 2011-04-08 [PMID: 21325271] (WB, Human)
  
  Details:
  TLR2/CD282 (IMG-410A). WB: TLR2 siRNA transfected human coronary artery endothelial (CAECs) cell line, Fig 3A. Note: The TLR2 (IMG-410A) antibody was siRNA validated by WB in CAEC cell line, Fig 3A.
• Ghanim H, Sia CL, Upadhyay M et al. Orange juice neutralizes the proinflammatory effect of a high-fat, high-carbohydrate meal and prevents endotoxin increase and Toll-like receptor expression. Am J Clin Nutr. 2010-04-01 [PMID: 20200256] (WB, Human)
• Wu H, Wang H, Xiong W et al. Expression patterns and functions of toll-like receptors in mouse sertoli cells. Endocrinology. 2008-09-01 [PMID: 18499758] (WB, Mouse)
  
  Details:
  Antibodies cited: 1. TLR2 (IMG-410A): WB (sertoli cell lysates), Fig. 1D. 2. TLR5 (IMG-444A): WB (sertoli cell lysates), Fig. 1D. 3. TLR5 (IMG-664C): Flow (cell surface), mouse sertoli cells and macrophages, Figs. 1B,C,E. 4. TLR6 (IMG-527): WB (sertoli ce
• Chun J, Prince A. Activation of Ca2+-dependent signaling by TLR2. J Immunol. 2006-07-15 [PMID: 16818794] (WB, Human)
  
  Details:
  1. TLR2 (IMG-410) [WB (human airway epithelial cell lines)].
• LeBouder E, Rey-Nores JE, Rushmere NK et al. Soluble forms of Toll-like receptor (TLR)2 capable of modulating TLR2 signaling are present in human plasma and breast milk. J Immunol. 2003-12-15 [PMID: 14662871] (WB, Human)
  
  Details:
  1. TLR2 (IMG-319 and IMG-410); For IMG-319: WB, Fig.1D and Fig.6C (Mono Mac-6 monocytes); For IMG-410: Fig.6A-B, (human breast milk).

Review for TLR2 Antibody (NB100-56720) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: cynthia morimoto WB Human 12/15/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID: 22963587
• Species: Human

Bioinformatics

• Gene Symbol: TLR2
• Entrez:
  • 7097(Human)
  • 24088(Mouse)
• Uniprot:
  • O60603()