TLR2 Antibodies: Novus Biologicals

NB100-56722

TLR2 Antibody (TL2.1) - BSA Free

(64 Publications)

0.1 mg

NB100-56722

Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Total protein from human THP-1 and U937 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TLR2 (NB100-56722) in 5% BSA-TBST and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).

<b>Biological Strategies Validation. </b>TLR2-Antibody-TL2-1-Flow-Cytometry-NB100-56722-img0013.jpg

Flow (Cell Surface): TLR2 Antibody (TL2.1) [NB100-56722] - Flow (Cell Surface): - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on stable transfected cell line using this antibody at 1 ug/10^6 cells. Green: isotype control. Red: anti-TLR2 antibody. Image using the Azide Free form of this antibody.

TLR2-Antibody-TL2-1-Block-Neutralize-NB100-56722-img0014.jpg

Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722] - Intracellular flow analysis of TLR2 in 1x10^6 PBMCs using 2 ug of NB100-56722. Shaded histogram represents cells without antibody; green represents isotype control; purple represents anti-TLR2 antibody.

Reactivity:

Hu, Mu, Rt, Ca, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Rb

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-416A

Alt Catalog# IMG-416A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56722AF350
Alexa Fluor 405	NB100-56722AF405
Alexa Fluor 488	NB100-56722AF488
Alexa Fluor 532	NB100-56722AF532
Alexa Fluor 594	NB100-56722AF594
Alexa Fluor 647	NB100-56722AF647
Alexa Fluor 700	NB100-56722AF700
Alexa Fluor 750	NB100-56722AF750
Alexa Fluor Plus 405	NB100-56722AFP405
Alexa Fluor Plus 488	NB100-56722AFP488
Alexa Fluor Plus 555	NB100-56722AFP555
Alexa Fluor Plus 594	NB100-56722AFP594
Alexa Fluor Plus 647	NB100-56722AFP647
Alexa Fluor Plus 680	NB100-56722AFP680
Allophycocyanin	NB100-56722APC
Biotin	NB100-56722B
CoraFluor 1	NB100-56722CL1
DyLight 594	NB100-56722DL594
FITC	NB100-56722F
Janelia Fluor 525	NB100-56722JF525
Janelia Fluor 549	NB100-56722JF549
Janelia Fluor 646	NB100-56722JF646
Janelia Fluor 669	NB100-56722JF669
PE	NBP2-24909
PE	NB100-56722PE
mFluor Violet 450 SE	NB100-56722MFV450
mFluor Violet 500 SE	NB100-56722MFV500
mFluor Violet 610 SE	NB100-56722MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB100-56726
BSA Free	NB100-56722SS
Azide and BSA Free	NB100-56726SS

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NB100-56720

TLR2 Antibody - BSA Free

(1 Review)

(20 Publications)

0.1 mg

NB100-56720

Immunohistochemistry-Paraffin: TLR2 Antibody [NB100-56720] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Analysis of a FFPE tissue section of human spleen using 1:200 dilution of TLR2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

TLR2 was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-TLR2 Protein G purified Polyclonal Antibody (Catalog # NB100-56720) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 40X objective.

TLR2 was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-TLR2 Protein G purified Polyclonal Antibody (Catalog # NB100-56720) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 40X objective.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-410A

Alt Catalog# IMG-410A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-24861
BSA Free	NB100-56720SS

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MAB2616

D8000C). IL-8 secretion elicited by Pam₃CSK₄(0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND₅₀ is typically 0.03-0.15 µg/mL." class="big_lightbox">

The synthetic tripalmitoylated lipopeptide Pam<SUB>3</SUB>CSK<SUB>4</SUB>stimulates IL-8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # <a class=

D8000C). IL-8 secretion elicited by Pam₃CSK₄(0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND₅₀ is typically 0.03-0.15 µg/mL." alt="The synthetic tripalmitoylated lipopeptide Pam₃CSK₄stimulates IL-8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). IL-8 secretion elicited by Pam₃CSK₄(0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND₅₀ is typically 0.03-0.15 µg/mL." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

Human whole blood monocytes were stained with Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616, filled histogram) or isotype control antibody (Catalog # <a class=

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2616U-100UG
Alexa Fluor 405	FAB2616V-100UG
Alexa Fluor 488	FAB2616G-100UG
Alexa Fluor 532	FAB2616X
Alexa Fluor 594	FAB2616T-100UG
Alexa Fluor 647	FAB2616R-100UG
Alexa Fluor 700	FAB2616N
Alexa Fluor 750	FAB2616S-100UG
Alexa Fluor Plus 405	FAB2616AFP405-100UG
Alexa Fluor Plus 488	FAB2616AFP488-100UG
Alexa Fluor Plus 555	FAB2616AFP555-100UG
Alexa Fluor Plus 594	FAB2616AFP594-100UG
Alexa Fluor Plus 647	FAB2616AFP647-100UG
Alexa Fluor Plus 680	FAB2616AFP680-100UG
Allophycocyanin	FAB2616A
Allophycocyanin/Cy7	FAB2616APCCY7
Biotin	FAB2616B
CoraFluor 1	FAB2616CL1
DyLight 350	FAB2616D
DyLight 405	FAB2616E
DyLight 488	FAB2616K
DyLight 550	FAB2616L
DyLight 594	FAB2616M
DyLight 650	FAB2616W
DyLight 680	FAB2616Y
DyLight 755	FAB2616Z
HRP	FAB2616H
Janelia Fluor 525	FAB2616JF525
Janelia Fluor 549	FAB2616I
Janelia Fluor 646	FAB2616J
Janelia Fluor 669	FAB2616JF669
PE/Atto594	FAB2616PEATT594
PE/Cy5.5	FAB2616PECY55
PE/Cy7	FAB2616PECY7
PerCP (Peridinin-chlorophyll Protein Complex)	FAB2616C
Phycoerythrin	FAB2616P
mFluor Violet 450 SE	FAB2616MFV450
mFluor Violet 500 SE	FAB2616MFV500
mFluor Violet 610 SE	FAB2616MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2616C
Carrier Free	FAB2616P
Carrier Free	FAB2616A
Carrier Free	FAB2616N
Carrier Free	MAB2616-SP
Carrier Free	FAB2616R-100UG
Carrier Free	FAB2616G-100UG
Carrier Free	FAB2616V-100UG
Carrier Free	FAB2616U-100UG
Carrier Free	FAB2616T-100UG
Carrier Free	FAB2616S-100UG

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AF2616

TLR2 Antibody [Unconjugated]

(18 Publications)

100 μg

AF2616

HAF017). A specific band was detected for TLR2 at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysate of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for TLR2 at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TLR2 at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

TLR2 expression in airway epithelial cells at baseline and after transfection with S1 or S2. BEAS-2B or A549 cells were cultured in a 24-well plate at 1 × 105 cells per well in a BEGM or F-12K medium (FBS, 10%) for 24 h. TLR2 mRNA and protein levels were assessed using RT-qPCR and immunoblotting, respectively. (a) TLR2 mRNA basal expression in BEAS-2B and A549 cells assessed, n = 3. Data are presented relative to control BEAS-2B cells ( delta delta Ct) as mean ± SEM. (b) Representative immunoblot and (c) densitometry analysis of basal TLR2 protein expression (92 kDa) in BEAS-2B and A549 cells, n = 3. Values were normalised to those of GAPDH (37 kDa) as a loading control. (d) TLR2 mRNA expression in BEAS-2B cells at baseline and after transfection with S1 or S2 subunit for 24 h, n = 3. The Ct value of TLR2 mRNA was normalised to that of 18S rRNA ( delta Ct). Data are presented relative to the control at baseline ( delta delta Ct). (e) Representative immunoblot of TLR2 protein and (f) densitometry analysis in BEAS-2B cells at baseline and after transfection with S1 or S2 subunit for 24 h, n = 4. Values were normalised to those of GAPDH as a loading control. Data are presented as mean ± SEM. * p ≤ 0.05, compared with the control group, using unpaired t-test or one-way analysis of variance with Bonferroni post-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38891051), licensed under a CC-BY license. Not internally tested by R&D Systems.

2616-TR) was serially diluted and captured by Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human TLR2 (Catalog # <a class=

2616-TR) was serially diluted and captured by Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2616-TR) was serially diluted and captured by Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

2616-TR) was serially diluted and captured by Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2616U-100UG
Alexa Fluor 405	AF2616V-100UG
Alexa Fluor 488	AF2616G-100UG
Alexa Fluor 532	AF2616X-100UG
Alexa Fluor 594	AF2616T-100UG
Alexa Fluor 647	AF2616R-100UG
Alexa Fluor 700	AF2616N-100UG
Alexa Fluor 750	AF2616S-100UG
Alexa Fluor Plus 405	AF2616AFP405-100UG
Alexa Fluor Plus 488	AF2616AFP488-100UG
Alexa Fluor Plus 555	AF2616AFP555-100UG
Alexa Fluor Plus 594	AF2616AFP594-100UG
Alexa Fluor Plus 647	AF2616AFP647-100UG
Alexa Fluor Plus 680	AF2616AFP680-100UG
Allophycocyanin	AF2616A
Biotin	BAF2616
CoraFluor 1	AF2616CL1
DyLight 350	AF2616D
DyLight 405	AF2616E
DyLight 488	AF2616K
DyLight 550	AF2616L
DyLight 594	AF2616M
DyLight 650	AF2616W
DyLight 680	AF2616Y
DyLight 755	AF2616Z
FITC	AF2616F
HRP	AF2616H
Janelia Fluor 525	AF2616JF525
Janelia Fluor 549	AF2616I
Janelia Fluor 646	AF2616J
Janelia Fluor 669	AF2616JF669
PE	AF2616P
PerCP	AF2616C
mFluor Violet 450 SE	AF2616MFV450
mFluor Violet 500 SE	AF2616MFV500
mFluor Violet 610 SE	AF2616MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2616-SP

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AF1530

TLR2 Antibody

(2 Reviews)

(9 Publications)

100 μg

AF1530

HAF019). A specific band was detected for TLR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for TLR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TLR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Impact of peroxisomal defect on the expression of phagocytosis-related genes in WT and mutant (Abcd1−/−Abcd2−/− and Acox1−/−) BV-2 cells. (A) Schematic representation of the main actors of phagocytosis. (B) Venn diagrams illustrating the up- and downregulated phagocytosis-related DEGs in Abcd1−/−Abcd2−/− and Acox1−/− non-stimulated BV-2 cells (DataSet GSE200022). Genes whose expression level was measured at the protein level are boxed. (C) Pie chart displaying the number of up- or downregulated phagocytosis-related DEGs after a 24-h LPS treatment (Log2FC > 1, AdjP Value < 0.05) in WT, Abcd1−/−Abcd2−/−, and Acox1−/− BV-2 cells (DataSet GSE237635). (D) Venn diagrams representing the LPS response of these DEGs (three independent batches of BV-2 cells for each genotype). (E) Heat map representing RNA-Seq gene expression of these DEGs at 24 h after LPS stimulation in WT, Abcd1−/−Abcd2−/−, and Acox1−/− BV-2 cells. (F) Representative image of Western blotting analysis (three independent experiments) of the expression levels of CD36, FCGR2B, MRC1, TLR2, and TLR4 in WT, Abcd1−/−Abcd2−/−, and Acox1−/− BV-2 cells untreated (–) or treated with LPS for 24 h (+). Expected molecular weights are indicated. Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fnmol.2023.1299314/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1530U-100UG
Alexa Fluor 405	AF1530V-100UG
Alexa Fluor 488	AF1530G-100UG
Alexa Fluor 532	AF1530X-100UG
Alexa Fluor 594	AF1530T-100UG
Alexa Fluor 647	AF1530R-100UG
Alexa Fluor 700	AF1530N-100UG
Alexa Fluor 750	AF1530S-100UG
Alexa Fluor Plus 405	AF1530AFP405-100UG
Alexa Fluor Plus 488	AF1530AFP488-100UG
Alexa Fluor Plus 555	AF1530AFP555-100UG
Alexa Fluor Plus 594	AF1530AFP594-100UG
Alexa Fluor Plus 647	AF1530AFP647-100UG
Alexa Fluor Plus 680	AF1530AFP680-100UG
Allophycocyanin	AF1530A
Biotin	BAF1530
CoraFluor 1	AF1530CL1
DyLight 350	AF1530D
DyLight 405	AF1530E
DyLight 488	AF1530K
DyLight 550	AF1530L
DyLight 594	AF1530M
DyLight 650	AF1530W
DyLight 680	AF1530Y
DyLight 755	AF1530Z
FITC	AF1530F
HRP	AF1530H
Janelia Fluor 525	AF1530JF525
Janelia Fluor 549	AF1530I
Janelia Fluor 646	AF1530J
Janelia Fluor 669	AF1530JF669
PE	AF1530P
PerCP	AF1530C
mFluor Violet 450 SE	AF1530MFV450
mFluor Violet 500 SE	AF1530MFV500
mFluor Violet 610 SE	AF1530MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1530-SP

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MAB1530

TLR2 Antibody (203325) [Unconjugated]

(2 Reviews)

(7 Publications)

500 μg

MAB1530

MAB0061, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

MAB0061, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B). View our protocol for Staining Membrane-associated Proteins. " alt="Raw264.7 cells stimulated with 100 ng/mL LPS overnight were stained with Rat Anti-Mouse TLR2 Monoclonal Antibody (Catalog # MAB1530, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

<b>Biological Strategies Validation. </b>TLR2Ab suppressed the expression of endogenous TLR2 agonists and interaction of TLR2 with HMGB1.(A) Expression of HMGB1 and Hsp70 were reduced in TLR2Ab-treated mice, but not in TLR4Ab group. (B) TLR2Ab and TLR4Ab suppressed its downstream signaling pathway. Data are shown as mean ± SEM (n = 5/group). (C) TLR2Ab inhibited the interaction of TLR2 with HMGB1 as indicated by co-immunoprecipitation and western blot. Data are representatives of two experiments with identical results. (D) TLR2Ab decreased the co-localization of TLR2 and HMGB1 as shown by confocal microscopy. Data are representatives of two experiments with identical results. #p<0.05, ##p<0.01, ###p<0.001 versus saline-treated group; *p<0.05, **p<0.01, ***p<0.001 versus Dox+IgG-treated group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22808256), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1530U-100UG
Alexa Fluor 405	FAB1530V-100UG
Alexa Fluor 488	FAB1530G-100UG
Alexa Fluor 532	FAB1530X
Alexa Fluor 594	FAB1530T-100UG
Alexa Fluor 647	FAB1530R-100UG
Alexa Fluor 700	FAB1530N-100UG
Alexa Fluor 750	FAB1530S-100UG
Alexa Fluor Plus 405	FAB1530AFP405-100UG
Alexa Fluor Plus 488	FAB1530AFP488-100UG
Alexa Fluor Plus 555	FAB1530AFP555-100UG
Alexa Fluor Plus 594	FAB1530AFP594-100UG
Alexa Fluor Plus 647	FAB1530AFP647-100UG
Alexa Fluor Plus 680	FAB1530AFP680-100UG
Allophycocyanin/Cy7	FAB1530APCCY7
Biotin	FAB1530B
CoraFluor 1	FAB1530CL1
DyLight 350	FAB1530D
DyLight 405	FAB1530E
DyLight 488	FAB1530K
DyLight 550	FAB1530L
DyLight 594	FAB1530M
DyLight 650	FAB1530W
DyLight 680	FAB1530Y
DyLight 755	FAB1530Z
Fluorescein	FAB1530F
HRP	FAB1530H
Janelia Fluor 525	FAB1530JF525
Janelia Fluor 549	FAB1530I
Janelia Fluor 646	FAB1530J
Janelia Fluor 669	FAB1530JF669
PE	FAB1530P
PE/Atto594	FAB1530PEATT594
PE/Cy5.5	FAB1530PECY55
PE/Cy7	FAB1530PECY7
PerCP	FAB1530C
mFluor Violet 450 SE	FAB1530MFV450
mFluor Violet 500 SE	FAB1530MFV500
mFluor Violet 610 SE	FAB1530MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1530F
Carrier Free	MAB1530-SP
Carrier Free	FAB1530V-100UG
Carrier Free	FAB1530U-100UG
Carrier Free	FAB1530T-100UG
Carrier Free	FAB1530S-100UG
Carrier Free	FAB1530R-100UG
Carrier Free	FAB1530N-100UG
Carrier Free	FAB1530G-100UG

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NB100-56586

TLR2 Antibody - BSA Free

(2 Publications)

0.1 mg

NB100-56586

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-662

Alt Catalog# IMG-662

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56586SS

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NBP2-25245

TLR2 Antibody (67N8F8)

(1 Publication)

0.1 mg

NBP2-25245

Flow (Cell Surface): TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis using the FITC conjugate of NBP2-25259. Surface staining of stable HEK293/hTLR2 cells (red) and vector control cells (green) using TLR2 antibody at 5 ul/10^6 cells. Image using the Azide Free form of this antibody.

Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of (A) TLR2 partial recombinant protein, (B) transfected cell lysate, and (C) control 293T lysate using TLR2 antibody at 1 ug/ml.

Flow Cytometry: TLR2 Antibody (67N8F8) [NBP2-25245] - (A) Surface staining of transfected TLR2 HEK293 cells (red) and mock transfected cells (green) using TLR2 antibody at 1 ug/10^6 cells. FITC-conjugated anti-mouse IgG secondary was used for this test. (B) Surface staining of PPI-negative PBMC (monocyte-gated) using TLR2 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells. PE-conjugated anti-mouse IgG secondary was used for this test.

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6925A

Alt Catalog# IMG-6925A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25259AF350
Alexa Fluor 405	NBP2-25259AF405
Alexa Fluor 488	NBP2-25259AF488
Alexa Fluor 532	NBP2-25259AF532
Alexa Fluor 594	NBP2-25259AF594
Alexa Fluor 647	NBP2-25259AF647
Alexa Fluor 700	NBP2-25259AF700
Alexa Fluor 750	NBP2-25259AF750
Alexa Fluor Plus 405	NBP2-25259AFP405
Alexa Fluor Plus 488	NBP2-25259AFP488
Alexa Fluor Plus 555	NBP2-25259AFP555
Alexa Fluor Plus 594	NBP2-25259AFP594
Alexa Fluor Plus 647	NBP2-25259AFP647
Alexa Fluor Plus 680	NBP2-25259AFP680
Allophycocyanin	NBP2-25259APC
Biotin	NBP2-25259B
DyLight 350	NBP2-25259UV
DyLight 405	NBP2-25259V
DyLight 488	NBP2-25259G
DyLight 550	NBP2-25259R
DyLight 594	NBP2-25245DL594
DyLight 650	NBP2-25259C
DyLight 680	NBP2-25259FR
DyLight 755	NBP2-25259IR
FITC	NBP2-25259F
HRP	NBP2-25259H
Janelia Fluor 549	NBP2-25259JF549
Janelia Fluor 646	NBP2-25259JF646
PE	NBP2-25259PE
PerCP	NBP2-25259PCP
mFluor Violet 450 SE	NBP2-25259MFV450
mFluor Violet 500 SE	NBP2-25259MFV500
mFluor Violet 610 SE	NBP2-25259MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-25259
Azide and BSA Free	NBP2-25259SS

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NBP2-27165

TLR2 Antibody (11G5) - BSA Free

(2 Publications)

0.1 mg

NBP2-27165

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Alt Catalog#

IMG-6720A

Alt Catalog# IMG-6720A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-27166AF350
Alexa Fluor 405	NBP2-27166AF405
Alexa Fluor 405	NBP2-27165AF405
Alexa Fluor 488	NBP2-27165AF488
Alexa Fluor 488	NBP2-27166AF488
Alexa Fluor 532	NBP2-27166AF532
Alexa Fluor 594	NBP2-27166AF594
Alexa Fluor 647	NBP2-27166AF647
Alexa Fluor 700	NBP2-27166AF700
Alexa Fluor 750	NBP2-27166AF750
Alexa Fluor Plus 405	NBP2-27166AFP405
Alexa Fluor Plus 488	NBP2-27166AFP488
Alexa Fluor Plus 555	NBP2-27166AFP555
Alexa Fluor Plus 594	NBP2-27166AFP594
Alexa Fluor Plus 647	NBP2-27166AFP647
Alexa Fluor Plus 680	NBP2-27166AFP680
Allophycocyanin	NBP2-27166APC
Biotin	NBP2-27166B
DyLight 350	NBP2-27166UV
DyLight 405	NBP2-27166V
DyLight 488	NBP2-27166G
DyLight 550	NBP2-27166R
DyLight 594	NBP2-27165DL594
DyLight 650	NBP2-27166C
DyLight 680	NBP2-27166FR
DyLight 755	NBP2-27166IR
FITC	NBP2-27166F
HRP	NBP2-27166H
Janelia Fluor 549	NBP2-27166JF549
Janelia Fluor 646	NBP2-27166JF646
PE	NBP2-27166PE
PerCP	NBP2-27166PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-27166
BSA Free	NBP2-27165SS

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NB100-56573

TLR2 Antibody - BSA Free

(1 Review)

(4 Publications)

0.1 mg

NB100-56573

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-526

Alt Catalog# IMG-526

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56573SS

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NBP2-30097

TLR2 Antibody (T2.5)

(4 Publications)

50 μg

NBP2-30097

Embryonic ENS culture neurons express TLR2/4/9. a Agarose gel showing specific products of real-time PCR for the assayed genes in ENS culture (PC); water was used as a no-template control (H2O) and rat colon cDNA as positive control (+C). b TLR relative expression in ENS culture in basal conditions (n = 8). c Localisation of TLR in ENS culture ganglia. B-tubulin III was used instead of HuC/D as neuronal marker for colocalisation with TLR9 due to antibody detection incompatibilities. White arrows point to neuronal somas. Scale bars: 25 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27538577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-25244

TLR2 Antibody (67N17F8)

0.1 mg

NBP2-25244

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6924A

Alt Catalog# IMG-6924A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25244AF350
Alexa Fluor 405	NBP2-25244AF405
Alexa Fluor 488	NBP2-25244AF488
Alexa Fluor 532	NBP2-25244AF532
Alexa Fluor 594	NBP2-25244AF594
Alexa Fluor 647	NBP2-25244AF647
Alexa Fluor 700	NBP2-25244AF700
Alexa Fluor 750	NBP2-25244AF750
Allophycocyanin	NBP2-25244APC
Biotin	NBP2-25244B
CoraFluor 1	NBP2-25244CL1
DyLight 350	NBP2-25244UV
DyLight 405	NBP2-25244V
DyLight 488	NBP2-25244G
DyLight 550	NBP2-25244R
DyLight 594	NBP2-25244DL594
DyLight 650	NBP2-25244C
DyLight 680	NBP2-25244FR
DyLight 755	NBP2-25244IR
FITC	NBP2-25244F
HRP	NBP2-25244H
Janelia Fluor 525	NBP2-25244JF525
Janelia Fluor 549	NBP2-25244JF549
Janelia Fluor 646	NBP2-25244JF646
Janelia Fluor 669	NBP2-25244JF669
PE	NBP2-25244PE
PerCP	NBP2-25244PCP
mFluor Violet 450 SE	NBP2-25244MFV450
mFluor Violet 500 SE	NBP2-25244MFV500
mFluor Violet 610 SE	NBP2-25244MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80980

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

TLR2 Antibodies

TLR2 Antibody (TL2.1) - BSA Free

TLR2 Antibody - BSA Free

TLR2 Antibody (383936)

TLR2 Antibody [Unconjugated]

TLR2 Antibody

TLR2 Antibody (203325) [Unconjugated]

TLR2 Antibody - BSA Free

TLR2 Antibody (67N8F8)

TLR2 Antibody (11G5) - BSA Free

TLR2 Antibody - BSA Free

TLR2 Antibody (T2.5)

TLR2 Antibody (67N17F8)