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TLR6 Antibody (86B1153.2) - BSA Free

Images

 
Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) [NB100-56536] - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced ...read more
Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the PE conjugate of NB100-56536. Staining of TLR6 in Ramos cells using this antibody. 5 ug/10^6 cells. Shaded histogram represents Ramos ...read more
Flow Cytometry: TLR6 Antibody (86B1153.2) [NB100-56536] - Cell surface analysis of TLR6 antibody in 10^6 human monocytes using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
86B1153.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

TLR6 Antibody (86B1153.2) - BSA Free Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 408-424 of human TRL6.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TLR6
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Cell Surface) reported in scientific literature (PMID 24986635)
  • Flow (Intracellular)
  • Flow Cytometry 3 ug/10-6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot reported in scientific literature (PMID 17332440)
Agonist
Control
TLR6 Overexpression Lysate
Publications
Read Publications using
NB100-56536 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR6 Antibody (86B1153.2) - BSA Free

  • CD286 antigen
  • CD286
  • TLR6
  • toll-like receptor 6

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa. Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6. TLR6 is predominantly expressed in spleen, thymus, ovary, and lung.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, IHC

Publications for TLR6 Antibody (NB100-56536)(18)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: FLOW, Flow Cytometry Control, Flow-CS, IF/IHC, IHC, IHC-P, WB.


Filter By Application
FLOW
(2)
Flow Cytometry Control
(3)
Flow-CS
(3)
IF/IHC
(1)
IHC
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-56536 Applications Species
Sato Y, Wakita A, Maeda E et al. High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma Current oncology (Toronto, Ont.) 2023-05-04 [PMID: 37232814] (IHC, Human) IHC Human
Schoniger S, Grafe H, Wipplinger M, Schoon H. Expression of Toll-like receptors 2, 4 and 6 in the equine chorioallantois. BioMed Research International. 2018-11-25 [PMID: 30502912] (IF/IHC) IF/IHC
Schoniger S, Grafe H, Schoon H. Expression of Toll-like receptors 2, 4 and 6 in different cell populations of the equine endometrium. Veterinary Immunology and Immunopathology. 2017-01-22 [PMID: 28242004]
Renkonen J, Toppila-Salmi S, Joenvaara S et al. Expression of Toll-like receptors in nasal epithelium in allergic rhinitis APMIS. 2015-06-08 [PMID: 26061394] (IHC-P, Human)

Details:
TLR7 antibody (Imgenex IMG-581A) was used for IHC-P on nasal biopsies from healthy non-smoking volunteers both allergic to birch pollen and non-allergic controls. Nasal biopsies were collected before and after off-seasonal intranasal birch pollen or diluent challenge and the immunostaining assay implicated - 4um paraffin sections, antigen retrieval withTris-HCl pH 8.5, processing on Autostainer, use of primary at 1.67 ug/mL concentration, Rabbit/Mouse REAL EnVision Detection system and Peroxidase/DAB+ detection (Fig 1, Fig 2D ).
IHC-P Human
Lopez MC, Palmer BE, Lawrence DA. Naive T cells, unconventional NK and NKT cells, and highly responsive monocyte-derived macrophages characterize human cord blood Immunobiology 2014-06-11 [PMID: 24986635] (Flow-CS, Human)

Details:
This citation used the PE version of this antibody.
Flow-CS Human
Regan T, Nally K, Carmody R et al. Identification of TLR10 as a Key Mediator of the Inflammatory Response to Listeria monocytogenes in Intestinal Epithelial Cells and Macrophages. J Immunol 2013-12-15 [PMID: 24198280] (FLOW) FLOW
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897]

Details:
Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
Oberg HH, Ly TT, Ussat S et al. Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands. J Immunol. 2010-05-01 [PMID: 20363971] (Flow Cytometry Control)

Details:
Flow(intracellular) cytometry: TLR2/CD282 PE (IMG-416D) and TLR6/CD286 (IMG-304A). T cells and Tregs were separated from freshly isolated human PBMCs, Fig S3.
Flow Cytometry Control
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009-09-01 [PMID: 20196781] (Flow-CS, Human)

Details:
flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.
Flow-CS Human
Pietschmann K, Beetz S, Welte S et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009-09-01 [PMID: 19703014] (Flow Cytometry Control, Human) Flow Cytometry Control Human
Show All 18 Publications.

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Product General Protocols

View specific protocols for TLR6 Antibody (NB100-56536): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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WB Video Protocol

FAQs for TLR6 Antibody (NB100-56536) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for TLR6 Antibody (NB100-56536)

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Blogs on TLR6.


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Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance
By Jamshed Arslan, Pharm. D., PhD. Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Peri...  Read full blog post.

Exploring Various Studies on TLR6 Expression
The protein TLR6 is one member of the large Toll-like receptor (TLR) family, which governs the activation of the innate immunity system and pathogen recognition in cells. The TLR family is highly conserved from Drosophila to humans, and all the family...  Read full blog post.

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Bioinformatics

Gene Symbol TLR6
Entrez
Uniprot