TLR6 Antibody (86B1153.2) (NB100-56536)

Images

 
Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) [NB100-56536] - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced ...read more
Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the PE conjugate of NB100-56536. Staining of TLR6 in Ramos cells using this antibody. 5 ug/10^6 cells. Shaded histogram represents Ramos ...read more
Flow Cytometry: TLR6 Antibody (86B1153.2) [NB100-56536] - Cell surface analysis of TLR6 antibody in 10^6 human monocytes using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green ...read more
Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the FITC conjugate of NB100-56536. Staining of TLR6 in human lymphocytes using this antibody; red represents anti-TLR6 antibody.
Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the FITC conjugate of NB100-56536. Staining of TLR6 in Ramos cells using this antibody. Green represents isotype control (mouse IgG1-FITC); ...read more
Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the PE conjugate of NB100-56536. Staining of TLR6 in human PBMC cells using this antibody at 0.5 ug/10^6 cells. Shaded histogram represents ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, Flow-CS, Flow-IC, IHC, IHC-P
Clone
86B1153.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

TLR6 Antibody (86B1153.2) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 408-424 of human TRL6.
Specificity
TLR6 (86B1153.2)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TLR6
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 3 ug/10-6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Flow (Cell Surface)
  • Flow (Intracellular)
Application Notes
Immunohistochemistry-Paraffin and Flow cytometry/(Intracellular). Use in Flow cell surface reported in scientific literature (PMID 24986635)
Agonist
Positive Control
TLR6 Lysate (NBL1-16955)
Publications
Read Publications using
NB100-56536 in the following applications:

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for TLR6 Antibody (86B1153.2)

  • CD286 antigen
  • CD286
  • TLR6
  • toll-like receptor 6

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa. Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6. TLR6 is predominantly expressed in spleen, thymus, ovary, and lung.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-56536
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Supplier Logo

Publications for TLR6 Antibody (NB100-56536)(18)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: FLOW, Flow, Flow-CS, Flow-IC, IHC-P, WB.


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FLOW
(2)
Flow
(1)
Flow-CS
(4)
Flow-IC
(3)
IHC-P
(1)
WB
(1)
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Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-56536 Applications Species
Schoniger S, Grafe H, Schoon H. Expression of Toll-like receptors 2, 4 and 6 in different cell populations of the equine endometrium. Veterinary Immunology and Immunopathology. Jan 22 2017 [PMID: 28242004]
Renkonen J, Toppila-Salmi S, Joenvaara S et al. Expression of Toll-like receptors in nasal epithelium in allergic rhinitis APMIS. 2015 Jun 08 [PMID: 26061394] (IHC-P, Human)

Details:
TLR6 antibody (Imgenex IMG-304A) was used for IHC-P on nasal biopsies from healthy non-smoking volunteers both allergic to birch pollen and non-allergic controls. Nasal biopsies were collected before and after off-seasonal intranasal birch pollen or diluent challenge and the immunostaining assay implicated - 4um paraffin sections, antigen retrieval withTris-HCl pH 8.5, processing on Autostainer, use of primary at 0.6 ug/mL concentration, Rabbit/Mouse REAL EnVision Detection system and Peroxidase/DAB+ detection (Fig 1, Fig 3).
IHC-P Human
Lopez MC, Palmer BE, Lawrence DA. Naive T cells, unconventional NK and NKT cells, and highly responsive monocyte-derived macrophages characterize human cord blood Immunobiology 2014 Jun 11 [PMID: 24986635] (Flow-CS, Human)

Details:
This citation used the FITC version of this antibody.
Flow-CS Human
Regan T, Nally K, Carmody R et al. Identification of TLR10 as a Key Mediator of the Inflammatory Response to Listeria monocytogenes in Intestinal Epithelial Cells and Macrophages. J Immunol 2013 Dec 15 [PMID: 24198280] (FLOW) FLOW
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013 Jan 1 [PMID: 23183897]

Details:
Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
Oberg HH, Ly TT, Ussat S et al. Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands. J Immunol. 2010 May 1 [PMID: 20363971] (Flow-IC)

Details:
Flow(intracellular) cytometry: TLR2/CD282 PE (IMG-416D) and TLR6/CD286 (IMG-304A). T cells and Tregs were separated from freshly isolated human PBMCs, Fig S3.
Flow-IC
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009 Sep [PMID: 20196781] (Flow-CS, Human)

Details:
The following antibodies were used in flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.
Flow-CS Human
Pietschmann K, Beetz S, Welte S et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009 Sep [PMID: 19703014] (Flow-IC, Human)

Details:
Flow (cell surface), Human T cell subsets (V sigma 1 and V sigma 2) isolated from PBMCs, Fig 3. 2. TLR6/CD286 (IMG-304A) & TLR8/CD288 PE (IMG-321D): Flow (intracellular), Human T cell subsets (V sigma 1 and V sigma 2) isolated from PBMCs, Fig 3.
Flow-IC Human
Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008 Mar 1 [PMID: 18292566]

Details:
Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
Peiser M, Koeck J, Kirschning CJ et al. Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity. J Leukoc Biol. 2008 May [PMID: 18252867] (Flow-CS)

Details:
IMG-304A Flow (Cell Surface), Fig. 1C [primary human Langerhans cells (LC), LC cells generated from monocytes, and dentric cells generated from monocytes].
Flow-CS
Show All 18 Publications.

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Product General Protocols

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FAQs for TLR6 Antibody (NB100-56536) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56536AF405
Alexa Fluor 488 Labeled NB100-56536AF488
Alexa Fluor 647 Labeled NB100-56536AF647
Alexa Fluor 700 Labeled NB100-56536AF700
Allophycocyanin Labeled NB100-56536APC
Biotin Labeled NBP2-24791
DyLight 350 Labeled NB100-56536UV
DyLight 405 Labeled NB100-56536V
DyLight 488 Labeled NB100-56536G
DyLight 550 Labeled NB100-56536R
DyLight 650 Labeled NB100-56536C
DyLight 680 Labeled NB100-56536FR
DyLight 755 Labeled NB100-56536IR
HRP Labeled NB100-56536H
PE Labeled NBP2-24969
PerCP Labeled NB100-56536PCP

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Blogs on TLR6.

Exploring Various Studies on TLR6 Expression
The protein TLR6 is one member of the large Toll-like receptor (TLR) family, which governs the activation of the innate immunity system and pathogen recognition in cells. The TLR family is highly conserved from Drosophila to humans, and all the family...  Read full blog post.

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Bioinformatics

Gene Symbol TLR6
Entrez
Uniprot