Pam3CSK4, TLR1 and TLR2 Ligand

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Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were ...read more
Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Evaluation of the ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line. IML-102 cell line is a stably co-transfected cell line that expresses full-length human ...read more
Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Differentiated human macrophages were treated with or without 50 ng/mL Pam3CSK4 for 2 h, and the release of IL-6 in culture supernatants of cells was measured by ELISA kit.

Product Details

Summary
Reactivity Hu, Mu, Ba, ChSpecies Glossary
Applications Func, In vitro, In vivo, LA
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

Pam3CSK4, TLR1 and TLR2 Ligand Summary

Description
Pam3CSK4, TLR1 and TLR2 Ligand is a synthetic tripalmitoylated lipohexapeptide analog of the immunologically active N-terminal portion of bacterial lipoprotein. It activates monocytes and macrophages and is also a potent activator of proinflammatory transcription factor NF-kB. Pam3CSK4, TLR1 and TLR2 Ligand is recognized by a heterodimer formed between TLR1 and TLR2.Pam3CSK4, TLR1 and TLR2 Ligand stimulation: If your cell line does not naturally express TLR1+ TLR2, co-transfect with plasmids pCMV/TLR1and pCMV/TLR2. Forty-eight hrs after transfection, stimulate cells with 10 to 100 ng/ml of Pam3CSK4 for 6-24 hrs. Determine Pam3CSK4 stimulation using appropriate detection assays.
Specificity
Pam3CSK4, TLR1 and TLR2 Ligand

Applications/Dilutions

Dilutions
  • Functional
  • In vitro assay
  • In vivo assay
  • Ligand Activation
Reviewed Applications
Read 1 Review rated 5
using
NBP2-25297 in the following applications:

Publications
Read Publications using
NBP2-25297 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24105263). Mouse reactivity reported in scientific literature (PMID: 25511699) Use in Chicken reported in scientific publication (PMID: 32733155). Use in Bacteria reported in scientific publication (PMID: 31482074).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Sterile Water
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

Solubility: Soluble in pyrogen-free deionized water, DMSO or aqueous buffers at pH <7.5. Reconstitution: Reconstitute with 2 ml pyrogen-free deionized water in a pyrogen-free tube. Reconstitution can also be performed in DMSO (1mg/ml stock solution). Homogenize by vortexing or use a homogenizer or sonicator to prepare a homogenous solution. Further dilutions can be made with cell culture medium or pyrogen-free deionized water. PBS is not recommended for reconstitution or dilution.

Alternate Names for Pam3CSK4, TLR1 and TLR2 Ligand

  • Pam3CSK4 TLR1 and TLR2 Ligand
  • Pam3CSK4
  • TLR1 and TLR2 Ligand

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Pam3CSK4, TLR1 and TLR2 Ligand (NBP2-25297)(29)

We have publications tested in 4 confirmed species: Human, Mouse, Bacteria, Chicken.

We have publications tested in 4 applications: Func, In vitro, In-vitro, LA.


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Func
(9)
In vitro
(6)
In-vitro
(1)
LA
(5)
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Human
(9)
Mouse
(8)
Bacteria
(1)
Chicken
(2)
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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NBP2-25297 Applications Species
Horii T, Orikawa Y, Ohira Y et al. Peptidoglycan-Like Components in Z-100, Extracted from Mycobacterium tuberculosis Strain Aoyama B, Increase IL-12p40 via NOD2 Journal of Immunology Research Jun 22 2022 [PMID: 35785036] (LA) LA
Kawahara T, Ito A, Kiso A, Kawamoto F Inhibitory effect of strawberry geranium (Saxifraga stolonifera) on Toll-like receptor 2-mediated inflammatory response in human skin keratinocytes Journal of Ethnopharmacology Apr 1 2021 [PMID: 33819504]
Terada T, Nii T, Isobe N, Yoshimura Y Effects of Toll-like Receptor Ligands on the Expression of Proinflammatory Cytokines and Avian beta-defensins in Cultured Chick Intestine J Poult Sci Jul 25 2020 [PMID: 32733155] (Chicken) Chicken
Kanoh H, Nitta T, Go S et al. Homeostatic and pathogenic roles of GM3 ganglioside molecular species in TLR4 signaling in obesity EMBO J. May 7 2020 [PMID: 32378734] (Mouse) Mouse
Effects of TLR Ligands on the Expression of Cytokines and Possible Role of NFkB in its Process in the Theca of Chicken Follicles Kang Y, Nii T, Isobe N J Poult Sci [PMID: 32055188] (Func, Chicken) Func Chicken
Yamaguchi M, Hirose Y, Takemura M, et al Streptococcus pneumoniae Evades Host Cell Phagocytosis and Limits Host Mortality Through Its Cell Wall Anchoring Protein PfbA. Front Cell Infect Microbiol. 2019 Aug 20 [PMID: 31482074] (Bacteria)

Details:
Mice were infected with S. pneumoniae.
Bacteria
Sun L, Hult EM, Cornell TT et al. Loss of myeloid-specific protein phosphatase 2A enhances lung injury and fibrosis and results in IL-10 dependent sensitization of epithelial cell apoptosis Am. J. Physiol. Lung Cell Mol. Physiol. Mar 6 2019 [PMID: 30838865] (Func, Mouse) Func Mouse
Nitta T, Kanoh H, Inamori KI et al. Globo-series glycosphingolipids enhance Toll-like receptor 4-mediated inflammation and play a pathophysiological role in diabetic nephropathy. Glycobiology. Nov 22 2018 [PMID: 30476082] (Mouse) Mouse
Nihashi Y, Ono T, Kagami H, Takaya T. Toll-like receptor ligand-dependent inflammatory responses in chick skeletal muscle myoblasts. J. Orthop. Res. Oct 31 2018 [PMID: 30389519]
Kikuchi J, Kuroda Y, Koyama D et al. Myeloma Cells are Activated in Bone Marrow Microenvironment by the CD180/MD-1 Complex which Senses Lipopolysaccharide Cancer Res. 2018 Jan 23 [PMID: 29363546] (Mouse) Mouse
Show All 29 Publications.

Review for Pam3CSK4, TLR1 and TLR2 Ligand (NBP2-25297) (1) 51

Average Rating: 5
(Based on 1 review)

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 Pam3CSK4, TLR1 and TLR2 Ligand NBP2-25297
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