Pam3CSK4, TLR1 and TLR2 Ligand

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Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were ...read more
Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Evaluation of the ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line. IML-102 cell line is a stably co-transfected cell line that expresses full-length human ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Func, In vitro, In vivo, LA
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

Pam3CSK4, TLR1 and TLR2 Ligand Summary

Immunogen
Pam3CSK4, TLR1 and TLR2 Ligand is a synthetic tripalmitoylated lipohexapeptide analog of the immunologically active N-terminal portion of bacterial lipoprotein. It activates monocytes and macrophages and is also a potent activator of proinflammatory transcription factor NF-kB. Pam3CSK4, TLR1 and TLR2 Ligand is recognized by a heterodimer formed between TLR1 and TLR2.Pam3CSK4, TLR1 and TLR2 Ligand stimulation: If your cell line does not naturally express TLR1+ TLR2, co-transfect with plasmids pCMV/TLR1and pCMV/TLR2. Forty-eight hrs after transfection, stimulate cells with 10 to 100 ng/ml of Pam3CSK4 for 6-24 hrs. Determine Pam3CSK4 stimulation using appropriate detection assays.
Specificity
Pam3CSK4, TLR1 and TLR2 Ligand

Applications/Dilutions

Application Notes
Activation of TLR1 and TLR2 heterodimer Stimulation of TLR1 and TLR2 has been reported with 10-100 ng/ml. Use in vitro assay reported in scientific literature (PMID: 24105263) Use in functional reported in scientific literature (PMID 25511699) Use in In-vivo reported in scientific literature (PMID 25466255). Use in Ligand activation reported in multiple pieces scientific literature.
Publications
Read Publications using
NBP2-25297 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24105263). Mouse reactivity reported in scientific literature (PMID: 25511699)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Sterile Water
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for Pam3CSK4, TLR1 and TLR2 Ligand

  • Pam3CSK4 TLR1 and TLR2 Ligand
  • Pam3CSK4
  • TLR1 and TLR2 Ligand

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Pam3CSK4, TLR1 and TLR2 Ligand (NBP2-25297)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Func, In vitro, In vivo, In-vitro, LA.


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Func
(8)
In vitro
(6)
In vivo
(1)
In-vitro
(2)
LA
(3)
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Human
(7)
Mouse
(8)
Rat
(1)
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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NBP2-25297 Applications Species
Nitta T, Kanoh H, Inamori KI et al. Globo-series glycosphingolipids enhance Toll-like receptor 4-mediated inflammation and play a pathophysiological role in diabetic nephropathy. Glycobiology. Nov 22 2018 [PMID: 30476082] (Mouse) Mouse
Nitta T, Kanoh H, Inamori KI et al. Globo-series glycosphingolipids enhance Toll-like receptor 4-mediated inflammation and play a pathophysiological role in diabetic nephropathy. Glycobiology. Nov 22 2018 [PMID: 30476082] (Mouse) Mouse
Kikuchi J, Kuroda Y, Koyama D et al. Myeloma Cells are Activated in Bone Marrow Microenvironment by the CD180/MD-1 Complex which Senses Lipopolysaccharide Cancer Res. 2018 Jan 23 [PMID: 29363546] (Mouse) Mouse
Parthasarathy G, Philipp MT. Receptor tyrosine kinases play a significant role in human oligodendrocyte inflammation and cell death associated with the Lyme disease bacterium Borrelia burgdorferi. J Neuroinflammation May 30 2017 [PMID: 28558791] (Human) Human
Wang P, Zeng F, He L et al. Alteration of the immune status of umbilical cord mesenchymal stem cells stimulated by TLR1/2 agonist, Pam3Csk Mol Med Rep 2016 Jul 13 [PMID: 27431529]
Yanai S, Tokuhara D, Tachibana D et al. Diabetic pregnancy activates the innate immune response through TLR5 or TLR1/2 on neonatal monocyte J. Reprod. Immunol. 2016 Jun 22 [PMID: 27351455] (In vitro, Human) In vitro Human
Croasdell A, Sime PJ, Phipps RP. Resolvin D2 decreases TLR4 expression to mediate resolution in human monocytes. FASEB J. Jun 2 2016 [PMID: 27256622]
Sigola LB, Fuentes AL, Millis LM et al. Effects of toll-Like receptor ligands on RAW 264.7 macrophage morphology and zymosan phagocytosis. Tissue and Cell. 2016 Apr 22 [PMID: 27157550] (Func, Mouse) Func Mouse
Mastorci K, Muraro E, Pasini E et al. Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma. PLoS ONE. Apr 29 2016 [PMID: 27123851] (Func, Human) Func Human
Phongsisay V, Iizasa E, Hara H, Yoshida H. Evidence for TLR4 and FcRgamma-CARD9 activation by cholera toxin B subunit and its direct bindings to TREM2 and LMIR5 receptors. Mol. Immunol. 2015 May 25 [PMID: 26021803] (LA, Func, In vitro, Human, Mouse)

Details:
Pam3CSK4, TLR1 and TLR2 Ligand was used at 50-100 ng/ml concentration for the stimulation/ligand activation application in experiments involving TLR4 reporter cell, W / MyD88?/? mouse bone marrow-derived macrophages /BMDMs and NF-?B reporter cells /T?BRed cells (reporter cells express a fluorescent marker DsRed under the control of NF-?B in human monocytes THP1). See full text for detailed protocols and results.
LA, Func, In vitro Human, Mouse
Show All 23 Publications.

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