Human CCL2/MCP-1 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Human CCL2/MCP-1 Quantikine ELISA Kit Summary

Background
The Quantikine Human MCP-1 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MCP-1 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human MCP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MCP-1. Results obtained using natural human ...MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-1.
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Specificity
Natural and recombinant human MCP-1
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
CCL2

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DCP00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL2/MCP-1 Quantikine ELISA Kit

  • C-C motif chemokine ligand 2
  • CCL2
  • GDCF-2
  • HC11
  • HSMCR30
  • MCAF
  • Mcp1
  • MCP-1
  • SCYA2
  • SMC-CF

Background

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL2/JE/MCP-1 (DCP00)(266)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Fish, Porcine, Primate - Chlorocebus aethiops (African Green Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).


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Human
(246)
Mouse
(2)
Rat
(4)
Fish
(1)
Porcine
(3)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(7)
All Species
Showing Publications 1 - 10 of 266. Show All 266 Publications.
Publications using DCP00 Applications Species
Karagyaur, M;Primak, A;Basalova, N;Monakova, A;Tolstoluzhinskaya, A;Kulebyakina, M;Chechekhina, E;Skryabina, M;Grigorieva, O;Chechekhin, V;Yakovleva, T;Turilova, V;Shagimardanova, E;Gazizova, G;Vigovskiy, M;Kulebyakin, K;Sysoeva, V;Dyachkova, U;Dzhauari, S;Bozov, K;Popov, V;Akopyan, Z;Efimenko, A;Kalinina, N;Tkachuk, V; Safety and Regenerative Properties of Immortalized Human Mesenchymal Stromal Cell Secretome International journal of molecular sciences 2025-09-24 [PMID: 41096593] (Human) Human
Vettoretti, S;Caldiroli, L;Molinari, P;Villa, A;Corsi Romanelli, MM;Vianello, E;Dozio, E;Genovesi, S; Exploring the Interplay Between Glycated Albumin, AGEs, and Inflammation in Old Patients with CKD Metabolites 2025-08-01 [PMID: 40863134] (Human) Human
Park, S;Jeong, I;Kim, OK; Ginsenoside Rh2 Mitigates Endoplasmic Reticulum Stress-Induced Apoptosis and Inflammation and Through Inhibition of Hepatocyte-Macrophage Inflammatory Crosstalk Nutrients 2025-05-15 [PMID: 40431422] (Human) Human
Ztriva, E;Moschonas, IC;Tselepis, A;Lambrou, D;Ntaios, G;Savopoulos, C;Kaiafa, G; Monocyte Chemoattractant Protein-1 as a Biomarker in Acute Ischemic Stroke: A Prospective Pilot Study Journal of clinical medicine 2025-05-09 [PMID: 40429291] (Human) Human
Chao, HW;Chao, WW;Chao, HM; Catalpol Protects Against Retinal Ischemia Through Antioxidation, Anti-Ischemia, Downregulation of ?-Catenin, VEGF, and Angiopoietin-2: In Vitro and In Vivo Studies International journal of molecular sciences 2025-04-24 [PMID: 40362263] (Rat) Rat
Parween, F;Singh, SP;Kathuria, N;Zhang, HH;Ashida, S;Otaizo-Carrasquero, FA;Shamsaddini, A;Gardina, PJ;Ganesan, S;Kabat, J;Lorenzi, HA;Riley, DJ;Myers, TG;Pittaluga, S;Bielekova, B;Farber, JM; Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines Nature communications 2025-02-26 [PMID: 40000641] (Human) Human
Lumpuy-Castillo, J;Rupérez, FJ;Porto, BLS;Cristóbal, C;Tarín, N;Huelmos, AI;Alonso, J;Egido, J;Mahíllo-Fernández, I;López-Bescós, L;Tuñón, J;Lorenzo, Ó; Plasma Levels of Propionylcarnitine Improved Prediction of Heart Failure and All-Cause Mortality in Patients with Stable Coronary Artery Disease Biomolecules 2024-12-29 [PMID: 39858422] (Human) Human
Nagel, S;Meyer, C;Pommerenke, C; IRX-related homeobox gene MKX is a novel oncogene in acute myeloid leukemia PloS one 2024-12-17 [PMID: 39689089] (Human) Human
Panda, K;Santiago, MJ;Rahman, MS;Ghorai, S;Black, SM;Rahman, I;Unwalla, HJ;Chinnapaiyan, S; HIV-1 Tat Protein and Cigarette Smoke Mediated ADAM17 Upregulation Can Lead to Impaired Mucociliary Clearance Cells 2024-12-05 [PMID: 39682757] (Human) Human
Wong, PY;Chan, CYK;Xue, HDG;Goh, CC;Cheu, JWS;Tse, APW;Zhang, MS;Zhang, Y;Wong, CCL; Cell cycle inhibitors activate hypoxia-induced DDX41-STING pathway to mediate anti-tumor immune response in liver cancer JCI insight 2024-10-10 [PMID: 39388278] (Human) Human
Show All 266 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CCL2/JE/MCP-1 (DCP00). (Showing 1 - 1 of 1 FAQs).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol CCL2