MyD88 Inhibitor Peptide Set

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Product Details

Summary
Reactivity Hu, Mu, Rt, Xp, Ye, ZeSpecies Glossary
Applications ELISA, Flow, Func-Inh, IHC, In vitro, In vivo, B/N
Concentration
LYOPH

Order Details

MyD88 Inhibitor Peptide Set Summary

Immunogen
Functions as a decoy by binding to the MyD88 TIR domain.
Preparation
Method
Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.
MyD88 Homodimerization Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRDVLPGT
Add 64.4 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.

Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing
*Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
Content
2 x 1.0mg: MyD88 Homodimerization Inhibitor peptide (lyophilized) DRQIKIWFQNRRMKWKKRDVLPGT (MyD88 homodimerization sequence: RDVLPGT). Molecular weight: 3100
2 x 1.0mg: Antennapedia Control peptide (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
5mg size will contain 5mg of each peptide (MyD88 Homodimerization Inhibitor peptide and Antennapedia Control peptide).
Gene
MYD88

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
Application Notes
Inhibition of MyD88 dependent TLR/IL-1R signaling activitiy by interefering with MyD88 homodimer formation.

Inhibitory peptide at 100 uM concentration may be a starting point. However, useful concentration of peptide may vary depending on experimental condition and cell type. Incubate cells for 24 hr with peptides before stimulating with ligands. Use in Functional inhibition reported in multiple pieces of scientific literature Use in In vivo assay reported in scientific literature (PMID 24755282) Use in blocking / neutralizing reported in scientific literature (PMID 24886588) Use in In vitro assay reported in scientific literature (PMID 25810567) Use in ELISA reported in scientific literature (PMID 23469306). Use in FLOW cytometry reported in scientific literature (PMID 27870876). Use in Binding inhibition reported in scientific literature (PMID 28302163). Use in Immunohistochemistry reported in scientific literature (PMID 28282921).
Reviewed Applications
Read 3 Reviews rated 5
using
NBP2-29328 in the following application:

Publications
Read Publications using
NBP2-29328 in the following applications:

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 27870876).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).
Concentration
LYOPH
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.

Notes

The MyD88 Homodimerization inhibitory peptide contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, The third helix of the antennapedia homeodomain translocates through biological membranes. J Biol Chem. 269:10444-10450 (1994)].The control peptide consists of only the PTD sequence.

Alternate Names for MyD88 Inhibitor Peptide Set

  • MyD88
  • MYD88D
  • myeloid differentiation primary response gene (88)
  • myeloid differentiation primary response protein MyD88

Background

MyD88 is an adapter protein that links Toll-like receptors (TLRs) and Interleukin-1 receptors (IL-1rs) with downstream signaling molecules. Homodimerization of MyD88 is a critical step in the signaling process, and allows the recruitment and activation of the kinase IRAK. MyD88 exists as a homodimer when recruited to activated TLR/IL-1Rs. The inhibitor peptide contains a sequence from the MyD88 TIR homodimerization domain (Loiarro, et al.). 1 MyD88 monomer binds to this inhibitor peptide, there by blocking MyD88 homodimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

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Publications for MyD88 Inhibitor (NBP2-29328)(75)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Yeast.

We have publications tested in 13 applications: B/N, BindInhib, ELISA, FLOW, Func, Func-INH, Func-Inh, Human, IHC, In vitro, In vivo, InhibAct, WB.


Filter By Application
B/N
(12)
BindInhib
(2)
ELISA
(2)
FLOW
(1)
Func
(2)
Func-INH
(2)
Func-Inh
(2)
Human
(1)
IHC
(2)
In vitro
(6)
In vivo
(4)
InhibAct
(1)
WB
(3)
All Applications
Filter By Species
Human
(12)
Mouse
(13)
Rat
(4)
Bovine
(1)
Porcine
(1)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 75. Show All 75 Publications.
Publications using NBP2-29328 Applications Species
Chang-Graham AL, Danhof HA, Engevik MA et al. Wnt5a is a TLR2/4-ligand that induces tolerance in human myeloid cells Commun Biol May 9 2019 [PMID: 31098409] (InhibAct, Human) InhibAct Human
Wang P, Liu BY, Wu MM et al. Moderate prenatal alcohol exposure suppresses the TLR4-mediated innate immune response in the hippocampus of young rats Neurosci. Lett. Jan 30 2019 [PMID: 30710662] (WB, Rat) WB Rat
Nguyen TNY, Padungros P, Wongsrisupphakul P et al. Cell wall mannan of Candida krusei mediates dendritic cell apoptosis and orchestrates Th17 polarization via TLR-2/MyD88-dependent pathway. Sci Rep. Nov 20 2018 [PMID: 30459422] (B/N, Mouse) B/N Mouse
Liu JT, Wu SX, Zhang H et al. Inhibition of MyD88 Signaling Skews Microglia/Macrophage Polarization and Attenuates Neuronal Apoptosis in the Hippocampus After Status Epilepticus in Mice Neurotherapeutics Aug 15 2018 [PMID: 30112701] (In vivo, Mouse) In vivo Mouse
Park SK, Jin SY, Yeon SH et al. Role of Toll-like receptor 9 signaling on activation of nasal polyp-derived fibroblasts and its association with nasal polypogenesis Int Forum Allergy Rhinol 2018 Jun 14 [PMID: 29901280]
Park SK, Jin SY, Yeon SH et al. Role of Toll-like receptor 9 signaling on activation of nasal polyp-derived fibroblasts and its association with nasal polypogenesis Int Forum Allergy Rhinol 2018 Jun 14 [PMID: 29901280]
Loetsch C, Warren J, Laskowski A et al. Cytosolic Recognition of RNA Drives the Immune Response to Heterologous Erythrocytes Cell Rep 2017 Nov 07 [PMID: 29117566] (B/N, Mouse) B/N Mouse
Dos Santos JC, Grund LZ, Seibert CS et al. Stingray venom activates IL-33 producing cardiomyocytes, but not mast cell, to promote acute neutrophil-mediated injury Sci Rep 2017 Aug 11 [PMID: 28801624] (B/N, Mouse) B/N Mouse
Ho MF, Ingle JN, Bongartz T et al. TCL1A SNPs and estrogen-mediated toll-like receptor-MYD88-dependent NF-kB activation: SNP and SERM-dependent modification of inflammation and immune response. Mol. Pharmacol. 2017 Jun 14 [PMID: 28615284]
Dreschers S, Saupp P, Hornef M et al. Reduced PICD in Monocytes Mounts Altered Neonate Immune Response to Candida albicans. PLoS ONE. Nov 21 2016 [PMID: 27870876] (FLOW, Yeast) FLOW Yeast
Show All 75 Publications.

Reviews for MyD88 Inhibitor (NBP2-29328) (3) 53

Average Rating: 5
(Based on 3 reviews)

Reviews using NBP2-29328:
Filter by Applications
ICC/IF
(3)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Parth Patel
ICC/IF 05/12/2017
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
 
reviewed by:
Kyong-su Park
ICC/IF 04/28/2017
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
 
reviewed by:
Parth Patel
ICC/IF 04/19/2017
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence

Product General Protocols

View specific protocols for MyD88 Inhibitor (NBP2-29328): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MyD88 Inhibitor (NBP2-29328). (Showing 1 - 4 of 4 FAQs).

  1. I have a question regarding the product NBP2-29328 and want to know how the inhibitory peptide passes through the cell wall?
    • The MyD88 inhibitory peptide contains a protein transduction (PTD) sequence derived from antennapedia which renders the PTD sequence renders the peptide cell permeable. The antennapedia technology was first described by Derossi et al, The third helix of the Antennapedia homeodomain translocates through biological membranes and is used in a number of our inhibitory peptides. The MyD88 inhibitory peptide also contains a sequence from the MyD88 TIR homodimerization domain (Loiarro et al, Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NF-{kappa}B). The control peptide consists of only the PTD sequence. The Derossi and Loiarro technology references are attached for additional background information. Please also follow this link to representative publications citing the MyD88 inhibitory peptide.
  2. I'm looking into purchasing NBP2-29328 for use and I am wondering if you can give me more information. Specifically, I would like to inject this drug ICM (directly into the CNS) using a rat model, and I am wondering what information you can provide about
    • Unfortunately, I do not see any in-vivo data for this product on our internal records. Most of the information that we have on our records for this product may be seen at its online datasheets : Novus Resources
  3. I was wondering whether NBP2-29328-5mg has biological activity.
    • Our MyD88 Inhibitor Peptide Set does have biological activity.
  4. Is this the same as ST2825 compound described by Loiarro et al, 2007?
    • ST2825 is the heptapeptide only, whereas the inhibitor peptide from NBP2-29328 corresponds to the association between the hexapeptide + the PTD sequence from antennapedia. In that case, this would suggest that the product used in Loiarro et al. is not the same as NBP2-29328.

Other Available Formats

NBP2-29328
NBP2-29328
NBP2-29328

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Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

MYD88 Expression and Tumorigenesis
MyD88, also called myeloid differentiation primary response gene 88, encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. The innate immune system recognizes the presence of bacterial pathogens thro...  Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

MYD88: Fanning Inflammation and Immune Responses
Myeloid differentiation primary response gene 88 (MYD88) encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. MYD88 protein functions as an essential signal transducer in the interleukin-1 and in To...  Read full blog post.

MyD88 Antibodies for IL Signaling and Immunity Research
The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

 
Parth Patel
05/12/2017
Application: ICC/IF
Species:

 
Kyong-su Park
04/28/2017
Application: ICC/IF
Species:

 
Parth Patel
04/19/2017
Application: ICC/IF
Species:

Bioinformatics

Gene Symbol MYD88