MyD88 Inhibitor Peptide Set


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Product Details

Reactivity Hu, Mu, Rt, Bv, Xp, Ye, ZeSpecies Glossary
Applications ELISA, Flow, Func-Inh, IHC, In vitro, In vivo, B/N

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MyD88 Inhibitor Peptide Set Summary

Functions as a decoy by binding to the MyD88 TIR domain.
Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.
MyD88 Homodimerization Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRDVLPGT
Add 64.4 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.

Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing
*Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
2 x 1.0mg: MyD88 Homodimerization Inhibitor peptide (lyophilized) DRQIKIWFQNRRMKWKKRDVLPGT (MyD88 homodimerization sequence: RDVLPGT). Molecular weight: 3100
2 x 1.0mg: Antennapedia Control peptide (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
5mg size will contain 5mg of each peptide (MyD88 Homodimerization Inhibitor peptide and Antennapedia Control peptide).


  • Binding Inhibition
  • Block/Neutralize
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Functional (Inhibition)
  • Immunohistochemistry
  • In vitro assay
  • In vivo assay
Application Notes
Inhibition of MyD88 dependent TLR/IL-1R signaling activitiy by interefering with MyD88 homodimer formation.

Inhibitory peptide at 100 uM concentration may be a starting point. However, useful concentration of peptide may vary depending on experimental condition and cell type. Incubate cells for 24 hr with peptides before stimulating with ligands. Use in Functional inhibition reported in multiple pieces of scientific literature Use in In vivo assay reported in scientific literature (PMID 24755282) Use in blocking / neutralizing reported in scientific literature (PMID 24886588) Use in In vitro assay reported in scientific literature (PMID 25810567) Use in ELISA reported in scientific literature (PMID 23469306). Use in FLOW cytometry reported in scientific literature (PMID 27870876) . Use in Binding inhibition reported in scientific literature (PMID 28302163). Use in Immunohistochemistry reported in scientific literature (PMID 28282921).
Reviewed Applications
Read 3 Reviews rated 5
NBP2-29328 in the following applications:

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NBP2-29328 in the following applications:

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 27870876).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.


The MyD88 Homodimerization inhibitory peptide contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, The third helix of the antennapedia homeodomain translocates through biological membranes. J Biol Chem. 269:10444-10450 (1994)] .The control peptide consists of only the PTD sequence.

Alternate Names for MyD88 Inhibitor Peptide Set

  • MyD88
  • MYD88D
  • myeloid differentiation primary response gene (88)
  • myeloid differentiation primary response protein MyD88


MyD88 is an adapter protein that links Toll-like receptors (TLRs) and Interleukin-1 receptors (IL-1rs) with downstream signaling molecules. Homodimerization of MyD88 is a critical step in the signaling process, and allows the recruitment and activation of the kinase IRAK. MyD88 exists as a homodimer when recruited to activated TLR/IL-1Rs. The inhibitor peptide contains a sequence from the MyD88 TIR homodimerization domain (Loiarro, et al.). 1 MyD88 monomer binds to this inhibitor peptide, there by blocking MyD88 homodimerization.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

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Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
Species: Hu, Mu
Applications: IHC, IHC-P, PEP-ELISA, WB
Species: Ca, Hu, Mu
Applications: BA, B/N, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: Flow, In vitro
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: B/N, In vitro
Species: Hu, Mu, Rt
Applications: BA, DB, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, KD, WB
Species: Mu
Applications: ELISA
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
Species: Hu
Applications: Bind
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
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Publications for MyD88 Inhibitor (NBP2-29328)(91)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Invertebrate, Porcine, Yeast.

We have publications tested in 13 applications: B/N, BindInhib, ELISA, FLOW, Func, Func-INH, Func-Inh, Human, IHC, In vitro, In vivo, InhibAct, WB.

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Showing Publications 1 - 10 of 91. Show All 91 Publications.
Publications using NBP2-29328 Applications Species
Chen M, Deng H, Zhao Y et al. Toll-Like Receptor 2 Modulates Pulmonary Inflammation and TNF-alpha Release Mediated by Mycoplasma pneumoniae Frontiers in Cellular and Infection Microbiology Mar 17 2022 [PMID: 35372108] (Func-Inh) Func-Inh
Kim Y, Jo M, Schmidt J, et al. Enhanced Potency of GalNAc-conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models Molecular Therapy 2019 Jun 28 (IHC, Mouse) IHC Mouse
Jacobovitz MR, Rupp S, Voss PA Dinoflagellate symbionts escape vomocytosis by host cell immune suppression bioRxiv Dec 6 2019 (Invertebrate) Invertebrate
Munoz-Flores C, Astuya-Villalon A, Romero A, Toledo J Salmonid MyD88 is a key adapter protein that activates innate effector mechanisms through the TLR5M/TLR5S signaling pathway and protects against Piscirickettsia salmonis infection Fish & Shellfish Immunology Jan 1 2022 [PMID: 34998987]
Creismeas A, Gazaille C, Bourdon A et al. TRPC3, but not TRPC1, as a good therapeutic target for standalone or complementary treatment of DMD Journal of translational medicine Dec 20 2021 [PMID: 34930315]
Sakai H, Tanaka Y, Tanaka A, Ohdan H TLR-MyD88 signaling blockades inhibit refractory B-1b cell immune responses to transplant-related glycan antigens Am. J. Transplant. Aug 31 2020 [PMID: 32865877]
Swaim CD, Canadeo LA, Monte KJ et al. Modulation of Extracellular ISG15 Signaling by Pathogens and Viral Effector Proteins Cell Rep Jun 16 2020 [PMID: 32553163] (B/N, Human) B/N Human
Noya M Searching for pattern recognition receptor (s) involved in the response to tick-borne encephalitis virus (TBEV) and the TBEV vaccine Thesis Jan 1 2020
Onishi K, Mochizuki J, Sato A et al. Total RNA and genomic DNA of Lactobacillus gasseri OLL2809 induce interleukin-12 production in the mouse macrophage cell line J774.1 via toll-like receptors 7 and 9 BMC Microbiol. Jul 20 2020 [PMID: 32689952] (Mouse) Mouse
Show All 91 Publications.

Reviews for MyD88 Inhibitor (NBP2-29328) (3) 53

Average Rating: 5
(Based on 3 reviews)

Reviews using NBP2-29328:
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Product General Protocols

View specific protocols for MyD88 Inhibitor (NBP2-29328): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MyD88 Inhibitor (NBP2-29328). (Showing 1 - 4 of 4 FAQs).

  1. I have a question regarding the product NBP2-29328 and want to know how the inhibitory peptide passes through the cell wall?
    • The MyD88 inhibitory peptide contains a protein transduction (PTD) sequence derived from antennapedia which renders the PTD sequence renders the peptide cell permeable. The antennapedia technology was first described by Derossi et al, The third helix of the Antennapedia homeodomain translocates through biological membranes and is used in a number of our inhibitory peptides. The MyD88 inhibitory peptide also contains a sequence from the MyD88 TIR homodimerization domain (Loiarro et al, Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NF-{kappa}B). The control peptide consists of only the PTD sequence. The Derossi and Loiarro technology references are attached for additional background information. Please also follow this link to representative publications citing the MyD88 inhibitory peptide.
  2. I'm looking into purchasing NBP2-29328 for use and I am wondering if you can give me more information. Specifically, I would like to inject this drug ICM (directly into the CNS) using a rat model, and I am wondering what information you can provide about
    • Unfortunately, I do not see any in-vivo data for this product on our internal records. Most of the information that we have on our records for this product may be seen at its online datasheets : Novus Resources
  3. I was wondering whether NBP2-29328-5mg has biological activity.
    • Our MyD88 Inhibitor Peptide Set does have biological activity.
  4. Is this the same as ST2825 compound described by Loiarro et al, 2007?
    • ST2825 is the heptapeptide only, whereas the inhibitor peptide from NBP2-29328 corresponds to the association between the hexapeptide + the PTD sequence from antennapedia. In that case, this would suggest that the product used in Loiarro et al. is not the same as NBP2-29328.

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The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

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Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

MYD88 Expression and Tumorigenesis
MyD88, also called myeloid differentiation primary response gene 88, encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. The innate immune system recognizes the presence of bacterial pathogens thro...  Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

MYD88: Fanning Inflammation and Immune Responses
Myeloid differentiation primary response gene 88 (MYD88) encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. MYD88 protein functions as an essential signal transducer in the interleukin-1 and in To...  Read full blog post.

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The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b...  Read full blog post.

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Gene Symbol MYD88