MALP-2, TLR6 and TLR2 ligand

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MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/ml of MALP2 as added. Cells ...read more
MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Evaluation of the MALP2 ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line.Cell line is a stably co-transfected cell line that expresses full-length human Toll-like ...read more

Product Details

Summary
Reactivity Hu, Mu, AvSpecies Glossary
Applications Func, In vitro
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

MALP-2, TLR6 and TLR2 ligand Summary

Immunogen
MALP-2, TLR6 and TLR2 ligand
Specificity
MALP-2 was originally isolated from Mycoplasma fermentans. This MALP-2 corresponds to the originally isolated isomer, which expresses potent endotoxin-like activity and approaches in certain experimental systems the toxicity of LPS. For description of the stereochemistry of MALP-2 please refer to M. Morr, et al. Eur. J. Immunol. 32, 3337 (2002). The importance of the stereochemistry of the central carbon atom of the diacylglycerol group has been described in the K.M. Omueti, et al. paper (2005), see below. Formula: C99H167N19O30S
Details of Functionality
Specific Activity is approx 2x10^8units/mg. One unit is defined as the dilution giving half maximal release of nitric oxide from C3H/HeJ mouse peritoneal exudate cells in the standard assay.

Applications/Dilutions

Application Notes
Source: Synthetic
Formula: C99H167N19O30S
MW: 2135.2 Use in In- vitro assay and functional reported in scientific literature (PMID 25466255)
Publications
Read Publications using
NBP2-26219 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24771854).Mouse reactivity reported in scientific literature (PMID: 25466255). Avian reactivity reported in scientific literature (PMID: 25967535)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
2 ug in 20 ul of sterile PBS solution containing 2.5% (v/v) 2-propanol, 25 mM n-octyl-b-D-glucopyranoside and 1% (wt/v) human serum albumin.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for MALP-2, TLR6 and TLR2 ligand

  • Macrophage-activating lipopeptide-2
  • MALP 2
  • MALP-2, TLR6 & TLR2 ligand
  • S-[2,3-bis(Palmityloxy)-(2R)-propyl-cysteinyl-GNNDESNISFKEK]
  • TLR activator MALP2

Background

MALP-2, TLR6 and TLR2 ligand sticks avidly to glass or plastic. Since it is active at very low concentrations, i.e. at high dilutions, these low amounts of material tend to become adsorbed to pipette tips and plastic or glass containers. To avoid this, prepare several dilution steps of the MALP-2, TLR6 and TLR2 ligand stock solution with medium containing 5% autologous serum or buffers with 2% HSA. The presence of 50mM octyl glucoside in the first dilution step is often beneficial and gives rise to higher activity. Avoid small volumes in large vessels. Do not filter.Because of the ester-bound fatty acids, MALP-2, TLR6 and TLR2 ligand is sensitive to alkali or acid. The medium should be preconditioned in the CO2 incubator in order to ensure that the pH does not become alkaline. Add cells in preconditioned medium as soon as possible and incubate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for MALP-2, TLR6 and TLR2 ligand (NBP2-26219)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Avian.

We have publications tested in 5 applications: Flow, Func, In vitro, In-vitro, LA.


Filter By Application
Flow
(1)
Func
(6)
In vitro
(5)
In-vitro
(1)
LA
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(3)
Avian
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP2-26219 Applications Species
Bi J, Koivisto L, Pang A et al. Suppression of alphavB6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells Sci Rep 2017 Jun 30 [PMID: 28667248] (In vitro, Human) In vitro Human
Battin C, Hennig A, Mayrhofer P et al. A human monocytic NF-kB fluorescent reporter cell line for detection of microbial contaminants in biological samples. PLoS ONE May 25 2017 [PMID: 28542462] (Flow, Human) Flow Human
Yanai S, Tokuhara D, Tachibana D et al. Diabetic pregnancy activates the innate immune response through TLR5 or TLR1/2 on neonatal monocyte J. Reprod. Immunol. 2016 Jun 22 [PMID: 27351455] (In vitro, Human) In vitro Human
Sigola LB, Fuentes AL, Millis LM et al. Effects of toll-Like receptor ligands on RAW 264.7 macrophage morphology and zymosan phagocytosis. Tissue and Cell. 2016 Apr 22 [PMID: 27157550] (Func, Mouse) Func Mouse
Heidegger S, Jarosch A, Schmickl M et al. Mycoplasma hyorhinis-Contaminated Cell Lines Activate Primary Innate Immune Cells via a Protease-Sensitive Factor. PLoS ONE. 2015 Nov 14 [PMID: 26565413] (In vitro, Func, Mouse)

Details:
MALP-2 (TLR6 and TLR2 ligand) was used at 500 ng/ml concentration for functional assays involving murine B16-F10 (B16) melanoma cell line.
In vitro, Func Mouse
Chen J, Ng MM, Chu JJ. Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection. PLoS Pathog. 2015 Jul 01 [PMID: 26226614] (Func, Human)

Details:
MALP-2, TLR6 and TLR2 ligand was used at 50 ng/ml concentration (alongwith mock or Dengue Virus DV2-infection and LPS ) for treatments of human PBMCs, HEK293 and wild-type or TLR6-/- murine peritoneal macrophages under various experimental conditions.
Func Human
Ghonime MG, Mitra S, Eldomany RA et al. Inflammasome priming is similar for francisella species that differentially induce inflammasome activation. PLoS ONE. 2015 May 21 [PMID: 25993107] (Func, Human) Func Human
Yong Y, Liu S, Hua G et al. Identification and functional characterization of Toll-like receptor 2-1 in geese. BMC Vet. Res. 2015 May 30 [PMID: 25967535] (Func, In vitro, Avian)

Details:
MALP-2, TLR6 and TLR2 ligand (a synthetic dipalmitoylated lipopeptide) was used at 10 ng/ml concentration in RPMI 1640 medium for 30 minutes stimulation of cultured peripheral blood mononuclear cells/PBMCs which were harvested from Magang geese.
Func, In vitro Avian
Godefroy E, Gallois A, Idoyaga J et al. Activation of Toll-like Receptor-2 by Endogenous Matrix Metalloproteinase-2 Modulates Dendritic-Cell-Mediated Inflammatory Responses. Cell Rep. 2014 Dec 11 [PMID: 25466255] (In-vitro, Func, Mouse) In-vitro, Func Mouse
Koziel J, Bryzek D, Sroka A et al. Citrullination Alters Immunomodulatory Function of LL-37 Essential for Prevention of Endotoxin-Induced Sepsis. J. Immunol. 2014 Jun 01 [PMID: 24771854] (LA, Human)

Details:
Human monocyte-derived macrophages stimulated with 10 ng/ml of MALP-2, Fig 7a.
LA Human
Show All 13 Publications.

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