MALP-2, TLR6 and TLR2 ligand Summary
Immunogen |
MALP-2, TLR6 and TLR2 ligand |
Specificity |
MALP-2 was originally isolated from Mycoplasma fermentans. This MALP-2 corresponds to the originally isolated isomer, which expresses potent endotoxin-like activity and approaches in certain experimental systems the toxicity of LPS. For description of the stereochemistry of MALP-2 please refer to M. Morr, et al. Eur. J. Immunol. 32, 3337 (2002). The importance of the stereochemistry of the central carbon atom of the diacylglycerol group has been described in the K.M. Omueti, et al. paper (2005), see below. Formula: C99H167N19O30S |
Details of Functionality |
Specific Activity is approx 2x10^8units/mg. One unit is defined as the dilution giving half maximal release of nitric oxide from C3H/HeJ mouse peritoneal exudate cells in the standard assay. |
Applications/Dilutions
Application Notes |
Source: Synthetic Formula: C99H167N19O30S MW: 2135.2 Use in In- vitro assay and functional reported in scientific literature (PMID 25466255) |
Publications |
Read Publications using NBP2-26219 in the following applications:
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Reactivity Notes
Bacteria reactivity reported in scientific literature (PMID:32849539). Human reactivity reported in scientific literature (PMID: 24771854)..Mouse reactivity reported in scientific literature (PMID: 25466255).. Avian reactivity reported in scientific literature (PMID: 25967535).
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
2 ug in 20 ul of sterile PBS solution containing 2.5% (v/v) 2-propanol, 25 mM n-octyl-b-D-glucopyranoside and 1% (wt/v) human serum albumin. |
Concentration |
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance. |
Notes
Disclaimer Note -Disclaimer Note - Human Serum- No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits. Human Serum- No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Alternate Names for MALP-2, TLR6 and TLR2 ligand
Background
MALP-2, TLR6 and TLR2 ligand sticks avidly to glass or plastic. Since it is active at very low concentrations, i.e. at high dilutions, these low amounts of material tend to become adsorbed to pipette tips and plastic or glass containers. To avoid this, prepare several dilution steps of the MALP-2, TLR6 and TLR2 ligand stock solution with medium containing 5% autologous serum or buffers with 2% HSA. The presence of 50mM octyl glucoside in the first dilution step is often beneficial and gives rise to higher activity. Avoid small volumes in large vessels. Do not filter.Because of the ester-bound fatty acids, MALP-2, TLR6 and TLR2 ligand is sensitive to alkali or acid. The medium should be preconditioned in the CO2 incubator in order to ensure that the pH does not become alkaline. Add cells in preconditioned medium as soon as possible and incubate.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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